Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000009.t1 | XP_003605525.1 | 68.5 | 232 | 72 | 1 | 1 | 232 | 1 | 231 | 1.70E-79 | 305.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000009.t1 | Q9FJK3 | 53.8 | 160 | 73 | 1 | 1 | 160 | 1 | 159 | 6.9e-42 | 172.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000009.t1 | G7JN85 | 68.5 | 232 | 72 | 1 | 1 | 232 | 1 | 231 | 1.2e-79 | 305.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene000009.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000009.t1 | MTR_4g032620 | 75.431 | 232 | 56 | 1 | 1 | 232 | 1 | 231 | 7.63e-128 | 360 |
| MS.gene000009.t1 | MTR_8g036130 | 58.065 | 248 | 87 | 4 | 1 | 232 | 1 | 247 | 4.31e-87 | 258 |
| MS.gene000009.t1 | MTR_4g031910 | 55.172 | 232 | 101 | 2 | 1 | 232 | 1 | 229 | 2.74e-77 | 233 |
| MS.gene000009.t1 | MTR_4g032290 | 52.245 | 245 | 90 | 4 | 1 | 232 | 1 | 231 | 5.43e-70 | 214 |
| MS.gene000009.t1 | MTR_4g032260 | 64.744 | 156 | 55 | 0 | 1 | 156 | 1 | 156 | 9.76e-60 | 186 |
| MS.gene000009.t1 | MTR_3g065100 | 48.707 | 232 | 110 | 4 | 1 | 224 | 1 | 231 | 1.42e-57 | 183 |
| MS.gene000009.t1 | MTR_3g466980 | 41.905 | 210 | 110 | 2 | 1 | 199 | 1 | 209 | 2.96e-52 | 169 |
| MS.gene000009.t1 | MTR_2g035610 | 66.116 | 121 | 41 | 0 | 1 | 121 | 1 | 121 | 5.52e-46 | 149 |
| MS.gene000009.t1 | MTR_3g466830 | 45.399 | 163 | 84 | 2 | 3 | 163 | 2 | 161 | 4.51e-45 | 148 |
| MS.gene000009.t1 | MTR_5g047580 | 42.308 | 156 | 88 | 2 | 1 | 156 | 1 | 154 | 3.16e-41 | 138 |
| MS.gene000009.t1 | MTR_1g090697 | 41.139 | 158 | 91 | 2 | 1 | 158 | 1 | 156 | 1.25e-38 | 131 |
| MS.gene000009.t1 | MTR_3g466890 | 42.949 | 156 | 88 | 1 | 3 | 158 | 2 | 156 | 7.33e-38 | 130 |
| MS.gene000009.t1 | MTR_4g063790 | 41.935 | 155 | 90 | 0 | 1 | 155 | 1 | 155 | 1.41e-37 | 129 |
| MS.gene000009.t1 | MTR_1g077390 | 39.744 | 156 | 92 | 2 | 1 | 156 | 1 | 154 | 9.70e-37 | 127 |
| MS.gene000009.t1 | MTR_1g090710 | 39.241 | 158 | 95 | 1 | 1 | 158 | 1 | 157 | 9.81e-37 | 127 |
| MS.gene000009.t1 | MTR_3g031100 | 38.788 | 165 | 101 | 0 | 1 | 165 | 1 | 165 | 3.35e-35 | 125 |
| MS.gene000009.t1 | MTR_1g084950 | 42.949 | 156 | 87 | 2 | 1 | 156 | 1 | 154 | 3.50e-35 | 122 |
| MS.gene000009.t1 | MTR_2g016210 | 37.805 | 164 | 102 | 0 | 1 | 164 | 1 | 164 | 6.14e-35 | 125 |
| MS.gene000009.t1 | MTR_7g011950 | 41.026 | 156 | 90 | 2 | 1 | 156 | 1 | 154 | 3.95e-34 | 120 |
| MS.gene000009.t1 | MTR_4g028800 | 79.167 | 72 | 13 | 1 | 1 | 72 | 1 | 70 | 4.80e-34 | 117 |
| MS.gene000009.t1 | MTR_1g090783 | 39.610 | 154 | 91 | 2 | 3 | 156 | 2 | 153 | 5.11e-34 | 120 |
| MS.gene000009.t1 | MTR_3g467080 | 37.179 | 156 | 98 | 0 | 1 | 156 | 1 | 156 | 7.23e-34 | 119 |
| MS.gene000009.t1 | MTR_4g028720 | 41.667 | 156 | 89 | 2 | 1 | 156 | 1 | 154 | 7.45e-33 | 117 |
| MS.gene000009.t1 | MTR_5g075380 | 38.961 | 154 | 93 | 1 | 1 | 154 | 1 | 153 | 2.00e-32 | 115 |
| MS.gene000009.t1 | MTR_3g031240 | 32.099 | 162 | 110 | 0 | 1 | 162 | 1 | 162 | 9.43e-30 | 109 |
| MS.gene000009.t1 | MTR_1g077320 | 36.538 | 156 | 97 | 2 | 1 | 156 | 1 | 154 | 3.54e-28 | 105 |
| MS.gene000009.t1 | MTR_7g055800 | 47.059 | 119 | 35 | 2 | 1 | 119 | 1 | 91 | 2.56e-26 | 98.2 |
| MS.gene000009.t1 | MTR_3g465410 | 39.683 | 126 | 76 | 0 | 29 | 154 | 1 | 126 | 8.10e-26 | 98.6 |
| MS.gene000009.t1 | MTR_1g077300 | 38.462 | 156 | 92 | 2 | 1 | 156 | 1 | 152 | 1.23e-25 | 98.2 |
| MS.gene000009.t1 | MTR_3g466900 | 37.795 | 127 | 78 | 1 | 32 | 158 | 2 | 127 | 1.77e-25 | 97.1 |
| MS.gene000009.t1 | MTR_3g466930 | 39.844 | 128 | 74 | 3 | 32 | 158 | 2 | 127 | 1.86e-25 | 97.1 |
| MS.gene000009.t1 | MTR_5g047560 | 35.256 | 156 | 83 | 3 | 1 | 156 | 1 | 138 | 3.36e-25 | 96.7 |
| MS.gene000009.t1 | MTR_2g035580 | 35.256 | 156 | 100 | 1 | 1 | 156 | 1 | 155 | 1.09e-23 | 93.2 |
| MS.gene000009.t1 | MTR_4g032270 | 49.000 | 100 | 45 | 4 | 135 | 232 | 36 | 131 | 3.71e-21 | 85.9 |
| MS.gene000009.t1 | MTR_7g106510 | 31.875 | 160 | 105 | 2 | 1 | 157 | 4 | 162 | 1.30e-20 | 90.1 |
| MS.gene000009.t1 | MTR_4g019670 | 35.897 | 117 | 75 | 0 | 3 | 119 | 6 | 122 | 1.40e-20 | 90.1 |
| MS.gene000009.t1 | MTR_7g055940 | 45.714 | 105 | 29 | 2 | 1 | 105 | 1 | 77 | 2.67e-20 | 82.0 |
| MS.gene000009.t1 | MTR_7g055790 | 45.455 | 110 | 42 | 1 | 1 | 110 | 59 | 150 | 3.15e-19 | 81.3 |
| MS.gene000009.t1 | MTR_3g093900 | 34.127 | 126 | 81 | 2 | 1 | 125 | 4 | 128 | 1.36e-16 | 78.6 |
| MS.gene000009.t1 | MTR_6g018920 | 30.000 | 160 | 97 | 6 | 1 | 154 | 1 | 151 | 5.52e-14 | 70.5 |
| MS.gene000009.t1 | MTR_1g114730 | 33.333 | 117 | 63 | 4 | 1 | 103 | 1 | 116 | 1.98e-12 | 65.9 |
| MS.gene000009.t1 | MTR_6g005450 | 35.593 | 118 | 61 | 5 | 1 | 104 | 1 | 117 | 9.00e-12 | 63.9 |
| MS.gene000009.t1 | MTR_2g035590 | 45.977 | 87 | 47 | 0 | 74 | 160 | 1 | 87 | 1.03e-11 | 60.1 |
| MS.gene000009.t1 | MTR_6g005440 | 36.441 | 118 | 60 | 5 | 1 | 104 | 1 | 117 | 1.52e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000009.t1 | AT5G48670 | 53.750 | 160 | 73 | 1 | 1 | 160 | 1 | 159 | 1.94e-49 | 164 |
| MS.gene000009.t1 | AT5G26630 | 39.267 | 191 | 114 | 2 | 1 | 190 | 1 | 190 | 4.39e-36 | 127 |
| MS.gene000009.t1 | AT1G65300 | 42.484 | 153 | 87 | 1 | 3 | 155 | 2 | 153 | 1.69e-35 | 127 |
| MS.gene000009.t1 | AT5G26650 | 40.523 | 153 | 90 | 1 | 4 | 156 | 2 | 153 | 1.39e-33 | 124 |
| MS.gene000009.t1 | AT5G27810 | 47.788 | 113 | 59 | 0 | 29 | 141 | 1 | 113 | 2.35e-33 | 117 |
| MS.gene000009.t1 | AT5G27960 | 46.610 | 118 | 63 | 0 | 4 | 121 | 2 | 119 | 4.41e-33 | 122 |
| MS.gene000009.t1 | AT3G05860 | 40.000 | 150 | 89 | 1 | 1 | 150 | 1 | 149 | 1.31e-32 | 117 |
| MS.gene000009.t1 | AT1G65330 | 39.205 | 176 | 94 | 3 | 3 | 173 | 2 | 169 | 1.98e-32 | 119 |
| MS.gene000009.t1 | AT3G05860 | 40.000 | 150 | 89 | 1 | 1 | 150 | 1 | 149 | 4.78e-32 | 117 |
| MS.gene000009.t1 | AT3G05860 | 40.000 | 150 | 89 | 1 | 1 | 150 | 1 | 149 | 5.54e-32 | 117 |
| MS.gene000009.t1 | AT1G31630 | 32.759 | 174 | 113 | 3 | 3 | 176 | 2 | 171 | 1.16e-28 | 110 |
| MS.gene000009.t1 | AT2G28700 | 36.184 | 152 | 92 | 2 | 1 | 150 | 1 | 149 | 1.36e-27 | 107 |
| MS.gene000009.t1 | AT1G31640 | 34.437 | 151 | 97 | 2 | 3 | 153 | 2 | 150 | 5.44e-27 | 108 |
| MS.gene000009.t1 | AT1G22590 | 30.769 | 156 | 104 | 1 | 1 | 156 | 1 | 152 | 2.15e-24 | 95.5 |
| MS.gene000009.t1 | AT5G26580 | 34.839 | 155 | 86 | 2 | 1 | 155 | 1 | 140 | 2.86e-23 | 96.3 |
| MS.gene000009.t1 | AT5G06500 | 36.364 | 154 | 96 | 1 | 1 | 154 | 1 | 152 | 1.78e-22 | 92.4 |
| MS.gene000009.t1 | AT2G40210 | 41.176 | 119 | 70 | 0 | 1 | 119 | 1 | 119 | 9.00e-20 | 87.0 |
| MS.gene000009.t1 | AT5G58890 | 38.384 | 99 | 53 | 3 | 1 | 92 | 1 | 98 | 4.07e-13 | 67.8 |
| MS.gene000009.t1 | AT5G55690 | 26.702 | 191 | 105 | 5 | 1 | 184 | 1 | 163 | 7.21e-13 | 66.6 |
| MS.gene000009.t1 | AT5G55690 | 26.702 | 191 | 105 | 5 | 1 | 184 | 1 | 163 | 7.21e-13 | 66.6 |
Find 0 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGAAAGTCAATCTTTTAT+GGG | + | 33294:47941-47960 | MS.gene000009:CDS | 20.0% |
| ! | AAAATTCAGAACAATGAATG+TGG | + | 33294:47553-47572 | MS.gene000009:CDS | 25.0% |
| ! | AAATTCAGAACAATGAATGT+GGG | + | 33294:47554-47573 | MS.gene000009:CDS | 25.0% |
| ! | AAGAGAAACTAAAGATACAA+AGG | + | 33294:47639-47658 | MS.gene000009:CDS | 25.0% |
| ! | TACATTCAGTAATGAAGAAA+TGG | + | 33294:47865-47884 | MS.gene000009:CDS | 25.0% |
| ! | TCTTCATTACTGAATGTAAA+TGG | - | 33294:47863-47882 | MS.gene000009:intergenic | 25.0% |
| ! | TTGATTCAAAACATCTTTGT+TGG | + | 33294:47747-47766 | MS.gene000009:CDS | 25.0% |
| !! | CATTGAAAGTCAATCTTTTA+TGG | + | 33294:47940-47959 | MS.gene000009:CDS | 25.0% |
| !! | TATGCTGAATAATTTGTCAT+TGG | + | 33294:47724-47743 | MS.gene000009:CDS | 25.0% |
| AATGAAGAAATGGCAATGAT+GGG | + | 33294:47875-47894 | MS.gene000009:CDS | 30.0% | |
| AATTCAGAACAATGAATGTG+GGG | + | 33294:47555-47574 | MS.gene000009:CDS | 30.0% | |
| TAATGAAGAAATGGCAATGA+TGG | + | 33294:47874-47893 | MS.gene000009:CDS | 30.0% | |
| TCATTACTGAATGTAAATGG+TGG | - | 33294:47860-47879 | MS.gene000009:intergenic | 30.0% | |
| AAGAGACTTACATGAACGAA+AGG | + | 33294:47603-47622 | MS.gene000009:CDS | 35.0% | |
| AAGCAACATACAATAGAAGG+CGG | + | 33294:47399-47418 | MS.gene000009:CDS | 35.0% | |
| AGAATCATCATCGACAATGA+AGG | - | 33294:47375-47394 | MS.gene000009:intergenic | 35.0% | |
| AGAGACTTACATGAACGAAA+GGG | + | 33294:47604-47623 | MS.gene000009:CDS | 35.0% | |
| AGTAGATTTGGCAAAACTCT+AGG | - | 33294:48019-48038 | MS.gene000009:intergenic | 35.0% | |
| ATGTTGTCGCCATTATTCAT+AGG | - | 33294:47923-47942 | MS.gene000009:intergenic | 35.0% | |
| ATTCAGAACAATGAATGTGG+GGG | + | 33294:47556-47575 | MS.gene000009:CDS | 35.0% | |
| CATGTAAGTCTCTTGATCAA+CGG | - | 33294:47597-47616 | MS.gene000009:intergenic | 35.0% | |
| GGAAAGCAACATACAATAGA+AGG | + | 33294:47396-47415 | MS.gene000009:CDS | 35.0% | |
| GTTGATTGACCACAAATTGA+AGG | + | 33294:47769-47788 | MS.gene000009:CDS | 35.0% | |
| TCATTGTCGATGATGATTCT+AGG | + | 33294:47375-47394 | MS.gene000009:CDS | 35.0% | |
| TTACTGAATGTAAATGGTGG+TGG | - | 33294:47857-47876 | MS.gene000009:intergenic | 35.0% | |
| ! | ATAGCTTGTGCCATAGTTTA+TGG | + | 33294:47470-47489 | MS.gene000009:CDS | 35.0% |
| ! | GAATAGTAATGGAGATGAGT+CGG | + | 33294:47973-47992 | MS.gene000009:CDS | 35.0% |
| ! | GATACAAAGGCATGATAACA+AGG | + | 33294:47652-47671 | MS.gene000009:CDS | 35.0% |
| !! | ATGTAAATGGTGGTGGTATT+TGG | - | 33294:47850-47869 | MS.gene000009:intergenic | 35.0% |
| !! | GGCTTGATGATGAATAGTAA+TGG | + | 33294:47962-47981 | MS.gene000009:CDS | 35.0% |
| !!! | TGAATTTTGAGAGCACTGTT+TGG | - | 33294:47541-47560 | MS.gene000009:intergenic | 35.0% |
| AACATACAATAGAAGGCGGA+AGG | + | 33294:47403-47422 | MS.gene000009:CDS | 40.0% | |
| AAGGCATGATAACAAGGAGA+TGG | + | 33294:47658-47677 | MS.gene000009:CDS | 40.0% | |
| ACATACAATAGAAGGCGGAA+GGG | + | 33294:47404-47423 | MS.gene000009:CDS | 40.0% | |
| GATTGACCACAAATTGAAGG+AGG | + | 33294:47772-47791 | MS.gene000009:CDS | 40.0% | |
| TACCAACCTCCTTCAATTTG+TGG | - | 33294:47781-47800 | MS.gene000009:intergenic | 40.0% | |
| TATGAACTAAGCACTCTGTG+TGG | + | 33294:47443-47462 | MS.gene000009:CDS | 40.0% | |
| TTGTCGCCATTATTCATAGG+CGG | - | 33294:47920-47939 | MS.gene000009:intergenic | 40.0% | |
| ! | CATGAACGAAAGGGTTCTAA+AGG | + | 33294:47613-47632 | MS.gene000009:CDS | 40.0% |
| !! | AATTGAAGGAGGTTGGTAGA+AGG | + | 33294:47783-47802 | MS.gene000009:CDS | 40.0% |
| CTCGTAGGGTCCATAAACTA+TGG | - | 33294:47483-47502 | MS.gene000009:intergenic | 45.0% | |
| GACCACAAATTGAAGGAGGT+TGG | + | 33294:47776-47795 | MS.gene000009:CDS | 45.0% | |
| GGAATGCCGCCTATGAATAA+TGG | + | 33294:47911-47930 | MS.gene000009:CDS | 45.0% | |
| ATCTCTGGTTGAGGCTCGTA+GGG | - | 33294:47497-47516 | MS.gene000009:intergenic | 50.0% | |
| TGGTAGAAGGTTGGAAGCAG+AGG | + | 33294:47796-47815 | MS.gene000009:CDS | 50.0% | |
| ! | CTAGGATCGACTTCGCCAAA+TGG | - | 33294:48001-48020 | MS.gene000009:intergenic | 50.0% |
| !! | GAAGGAGGTTGGTAGAAGGT+TGG | + | 33294:47787-47806 | MS.gene000009:CDS | 50.0% |
| ACGAGCCTCAACCAGAGATC+TGG | + | 33294:47498-47517 | MS.gene000009:CDS | 55.0% | |
| AGCACTGTTTGGACTCCCTG+TGG | - | 33294:47530-47549 | MS.gene000009:intergenic | 55.0% | |
| GATCTCTGGTTGAGGCTCGT+AGG | - | 33294:47498-47517 | MS.gene000009:intergenic | 55.0% | |
| GCGGAAGGGTCTACTGAAGA+AGG | + | 33294:47418-47437 | MS.gene000009:CDS | 55.0% | |
| GTTTGGACTCCCTGTGGTGA+CGG | - | 33294:47524-47543 | MS.gene000009:intergenic | 55.0% | |
| TCATAGGCGGCATTCCAGCA+TGG | - | 33294:47907-47926 | MS.gene000009:intergenic | 55.0% | |
| !! | ATGATGGGTCCTAGCCATGC+TGG | + | 33294:47890-47909 | MS.gene000009:CDS | 55.0% |
| !! | GGAGATGAGTCGGTGCCATT+TGG | + | 33294:47983-48002 | MS.gene000009:CDS | 55.0% |
| AGAGATCTGGCCGTCACCAC+AGG | + | 33294:47511-47530 | MS.gene000009:CDS | 60.0% | |
| GACGGCCAGATCTCTGGTTG+AGG | - | 33294:47506-47525 | MS.gene000009:intergenic | 60.0% | |
| GAGATCTGGCCGTCACCACA+GGG | + | 33294:47512-47531 | MS.gene000009:CDS | 60.0% | |
| !! | TGTGGTGACGGCCAGATCTC+TGG | - | 33294:47512-47531 | MS.gene000009:intergenic | 60.0% |
| GGCGGCATTCCAGCATGGCT+AGG | - | 33294:47902-47921 | MS.gene000009:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33294 | gene | 47347 | 48045 | 47347 | ID=MS.gene000009 |
| 33294 | mRNA | 47347 | 48045 | 47347 | ID=MS.gene000009.t1;Parent=MS.gene000009 |
| 33294 | exon | 47347 | 48045 | 47347 | ID=MS.gene000009.t1.exon1;Parent=MS.gene000009.t1 |
| 33294 | CDS | 47347 | 48045 | 47347 | ID=cds.MS.gene000009.t1;Parent=MS.gene000009.t1 |
| Gene Sequence |
| Protein sequence |
