Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000010.t1 | XP_003605494.2 | 81 | 242 | 33 | 1 | 4 | 245 | 3 | 231 | 5.60E-102 | 380.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000010.t1 | Q9FJK3 | 47.7 | 193 | 99 | 2 | 4 | 196 | 3 | 193 | 1.3e-38 | 161.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000010.t1 | G7JN54 | 81.0 | 242 | 33 | 1 | 4 | 245 | 3 | 231 | 4.1e-102 | 380.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene000010.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000010.t1 | MTR_4g031910 | 83.471 | 242 | 25 | 1 | 4 | 245 | 3 | 229 | 1.31e-138 | 388 |
| MS.gene000010.t1 | MTR_4g032290 | 80.992 | 242 | 33 | 1 | 4 | 245 | 3 | 231 | 6.16e-133 | 374 |
| MS.gene000010.t1 | MTR_8g036130 | 61.753 | 251 | 81 | 5 | 4 | 245 | 3 | 247 | 1.02e-98 | 288 |
| MS.gene000010.t1 | MTR_4g032620 | 58.367 | 245 | 88 | 4 | 1 | 245 | 1 | 231 | 1.40e-94 | 277 |
| MS.gene000010.t1 | MTR_4g032260 | 81.818 | 154 | 28 | 0 | 4 | 157 | 3 | 156 | 4.74e-82 | 243 |
| MS.gene000010.t1 | MTR_3g065100 | 48.548 | 241 | 117 | 4 | 4 | 242 | 3 | 238 | 1.86e-64 | 201 |
| MS.gene000010.t1 | MTR_3g466980 | 54.902 | 153 | 68 | 1 | 4 | 156 | 3 | 154 | 8.85e-59 | 186 |
| MS.gene000010.t1 | MTR_4g032270 | 57.616 | 151 | 43 | 2 | 95 | 245 | 2 | 131 | 3.42e-53 | 168 |
| MS.gene000010.t1 | MTR_2g035610 | 73.333 | 120 | 32 | 0 | 4 | 123 | 3 | 122 | 1.34e-50 | 161 |
| MS.gene000010.t1 | MTR_3g466830 | 44.720 | 161 | 88 | 1 | 4 | 164 | 2 | 161 | 2.04e-44 | 147 |
| MS.gene000010.t1 | MTR_5g075380 | 45.333 | 150 | 81 | 1 | 6 | 155 | 5 | 153 | 1.25e-42 | 142 |
| MS.gene000010.t1 | MTR_4g063790 | 41.104 | 163 | 96 | 0 | 4 | 166 | 3 | 165 | 3.86e-42 | 141 |
| MS.gene000010.t1 | MTR_3g466890 | 42.138 | 159 | 91 | 1 | 4 | 162 | 2 | 159 | 6.02e-40 | 135 |
| MS.gene000010.t1 | MTR_3g031100 | 42.038 | 157 | 90 | 1 | 1 | 157 | 1 | 156 | 4.54e-39 | 136 |
| MS.gene000010.t1 | MTR_5g047580 | 44.805 | 154 | 83 | 2 | 4 | 157 | 3 | 154 | 4.80e-39 | 133 |
| MS.gene000010.t1 | MTR_2g016210 | 41.447 | 152 | 89 | 0 | 6 | 157 | 5 | 156 | 1.18e-38 | 135 |
| MS.gene000010.t1 | MTR_3g467080 | 40.260 | 154 | 92 | 0 | 4 | 157 | 3 | 156 | 3.03e-38 | 131 |
| MS.gene000010.t1 | MTR_1g077390 | 40.909 | 154 | 89 | 2 | 4 | 157 | 3 | 154 | 9.07e-35 | 122 |
| MS.gene000010.t1 | MTR_1g090783 | 39.610 | 154 | 91 | 2 | 4 | 157 | 2 | 153 | 1.54e-34 | 121 |
| MS.gene000010.t1 | MTR_1g090710 | 39.623 | 159 | 94 | 2 | 1 | 159 | 1 | 157 | 3.16e-34 | 120 |
| MS.gene000010.t1 | MTR_1g090697 | 39.744 | 156 | 92 | 2 | 4 | 159 | 3 | 156 | 8.25e-34 | 119 |
| MS.gene000010.t1 | MTR_3g465410 | 40.146 | 137 | 82 | 0 | 30 | 166 | 1 | 137 | 5.09e-33 | 117 |
| MS.gene000010.t1 | MTR_1g077320 | 42.857 | 154 | 86 | 2 | 4 | 157 | 3 | 154 | 3.11e-32 | 115 |
| MS.gene000010.t1 | MTR_7g011950 | 42.857 | 154 | 86 | 2 | 4 | 157 | 3 | 154 | 3.17e-32 | 115 |
| MS.gene000010.t1 | MTR_1g084950 | 43.506 | 154 | 85 | 2 | 4 | 157 | 3 | 154 | 4.11e-32 | 115 |
| MS.gene000010.t1 | MTR_4g028720 | 41.558 | 154 | 88 | 2 | 4 | 157 | 3 | 154 | 1.25e-30 | 111 |
| MS.gene000010.t1 | MTR_4g028800 | 78.788 | 66 | 12 | 1 | 6 | 71 | 5 | 68 | 1.29e-30 | 108 |
| MS.gene000010.t1 | MTR_1g077300 | 42.857 | 154 | 84 | 2 | 4 | 157 | 3 | 152 | 2.94e-29 | 108 |
| MS.gene000010.t1 | MTR_3g466900 | 38.462 | 130 | 79 | 1 | 33 | 162 | 2 | 130 | 4.39e-29 | 107 |
| MS.gene000010.t1 | MTR_3g031240 | 33.333 | 159 | 105 | 1 | 1 | 159 | 1 | 158 | 6.15e-29 | 108 |
| MS.gene000010.t1 | MTR_7g055800 | 50.427 | 117 | 30 | 2 | 4 | 120 | 3 | 91 | 1.90e-28 | 103 |
| MS.gene000010.t1 | MTR_3g466930 | 36.154 | 130 | 82 | 1 | 33 | 162 | 2 | 130 | 6.84e-27 | 101 |
| MS.gene000010.t1 | MTR_4g019670 | 39.103 | 156 | 79 | 2 | 3 | 158 | 5 | 144 | 1.05e-26 | 107 |
| MS.gene000010.t1 | MTR_7g106510 | 38.462 | 156 | 80 | 2 | 3 | 158 | 5 | 144 | 1.12e-26 | 107 |
| MS.gene000010.t1 | MTR_3g093900 | 29.016 | 193 | 126 | 2 | 3 | 195 | 5 | 186 | 4.44e-25 | 103 |
| MS.gene000010.t1 | MTR_7g055790 | 53.211 | 109 | 33 | 1 | 4 | 112 | 61 | 151 | 8.83e-25 | 96.3 |
| MS.gene000010.t1 | MTR_2g035590 | 59.794 | 97 | 38 | 1 | 75 | 170 | 1 | 97 | 9.54e-25 | 94.7 |
| MS.gene000010.t1 | MTR_2g035580 | 35.099 | 151 | 97 | 1 | 7 | 157 | 6 | 155 | 7.38e-24 | 94.0 |
| MS.gene000010.t1 | MTR_7g055940 | 49.038 | 104 | 25 | 2 | 4 | 107 | 3 | 78 | 9.21e-23 | 89.0 |
| MS.gene000010.t1 | MTR_5g047560 | 35.065 | 154 | 82 | 3 | 4 | 157 | 3 | 138 | 8.76e-21 | 85.5 |
| MS.gene000010.t1 | MTR_6g018920 | 32.432 | 111 | 68 | 4 | 4 | 108 | 3 | 112 | 5.52e-12 | 65.1 |
| MS.gene000010.t1 | MTR_6g005450 | 35.965 | 114 | 65 | 5 | 1 | 108 | 1 | 112 | 7.15e-12 | 64.7 |
| MS.gene000010.t1 | MTR_4g084780 | 57.692 | 52 | 22 | 0 | 75 | 126 | 1 | 52 | 1.29e-11 | 61.2 |
| MS.gene000010.t1 | MTR_1g114730 | 32.432 | 111 | 68 | 3 | 4 | 108 | 3 | 112 | 1.44e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000010.t1 | AT5G48670 | 47.500 | 200 | 97 | 3 | 4 | 200 | 3 | 197 | 1.67e-46 | 157 |
| MS.gene000010.t1 | AT1G65300 | 40.761 | 184 | 95 | 3 | 4 | 187 | 2 | 171 | 1.47e-41 | 143 |
| MS.gene000010.t1 | AT1G65330 | 40.541 | 185 | 96 | 2 | 4 | 188 | 2 | 172 | 3.43e-39 | 137 |
| MS.gene000010.t1 | AT5G26630 | 41.379 | 203 | 102 | 3 | 4 | 204 | 3 | 190 | 9.64e-38 | 132 |
| MS.gene000010.t1 | AT5G26650 | 40.854 | 164 | 96 | 1 | 6 | 169 | 3 | 165 | 5.14e-37 | 134 |
| MS.gene000010.t1 | AT5G27960 | 40.244 | 164 | 97 | 1 | 6 | 169 | 3 | 165 | 2.46e-36 | 131 |
| MS.gene000010.t1 | AT1G31630 | 28.839 | 267 | 158 | 6 | 4 | 243 | 2 | 263 | 3.07e-35 | 128 |
| MS.gene000010.t1 | AT1G31640 | 39.073 | 151 | 90 | 2 | 4 | 154 | 2 | 150 | 5.34e-32 | 122 |
| MS.gene000010.t1 | AT5G27810 | 54.839 | 93 | 42 | 0 | 30 | 122 | 1 | 93 | 1.07e-30 | 110 |
| MS.gene000010.t1 | AT3G05860 | 39.189 | 148 | 89 | 1 | 4 | 151 | 3 | 149 | 4.68e-30 | 111 |
| MS.gene000010.t1 | AT3G05860 | 39.189 | 148 | 89 | 1 | 4 | 151 | 3 | 149 | 1.97e-29 | 111 |
| MS.gene000010.t1 | AT3G05860 | 39.189 | 148 | 89 | 1 | 4 | 151 | 3 | 149 | 1.97e-29 | 111 |
| MS.gene000010.t1 | AT2G28700 | 33.854 | 192 | 120 | 3 | 4 | 193 | 3 | 189 | 4.33e-28 | 109 |
| MS.gene000010.t1 | AT5G26580 | 36.538 | 156 | 83 | 3 | 1 | 156 | 1 | 140 | 4.30e-26 | 104 |
| MS.gene000010.t1 | AT1G22590 | 31.847 | 157 | 102 | 2 | 1 | 157 | 1 | 152 | 8.36e-25 | 96.7 |
| MS.gene000010.t1 | AT5G06500 | 34.868 | 152 | 97 | 2 | 4 | 155 | 3 | 152 | 1.90e-19 | 84.7 |
| MS.gene000010.t1 | AT2G40210 | 39.098 | 133 | 79 | 2 | 4 | 135 | 3 | 134 | 3.27e-17 | 80.1 |
| MS.gene000010.t1 | AT5G58890 | 38.583 | 127 | 67 | 5 | 7 | 124 | 6 | 130 | 5.20e-16 | 75.9 |
| MS.gene000010.t1 | AT5G55690 | 43.038 | 79 | 37 | 3 | 1 | 72 | 1 | 78 | 2.55e-12 | 65.5 |
| MS.gene000010.t1 | AT5G55690 | 43.038 | 79 | 37 | 3 | 1 | 72 | 1 | 78 | 2.55e-12 | 65.5 |
Find 0 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAACAAAGAAAAGAAAAT+AGG | + | 33294:50448-50467 | MS.gene000010:CDS | 20.0% |
| !! | ATAATGTCATCTTCATTAAT+AGG | - | 33294:50756-50775 | MS.gene000010:intergenic | 20.0% |
| !! | GAAACAAAGAAAAGAAAATA+GGG | + | 33294:50449-50468 | MS.gene000010:CDS | 20.0% |
| ! | CAATGATCAAAATCAAATCA+TGG | + | 33294:50620-50639 | MS.gene000010:CDS | 25.0% |
| ! | TATGCAAAGTCAATTATTGA+TGG | + | 33294:50773-50792 | MS.gene000010:CDS | 25.0% |
| AGTGAAGAAATGGCAATTAT+GGG | + | 33294:50711-50730 | MS.gene000010:CDS | 30.0% | |
| ATCATACAAGAAAAGAACGA+AGG | + | 33294:50200-50219 | MS.gene000010:CDS | 30.0% | |
| ATGAGTCAATGTCTTAATGA+AGG | + | 33294:50492-50511 | MS.gene000010:CDS | 30.0% | |
| TCATACAAGAAAAGAACGAA+GGG | + | 33294:50201-50220 | MS.gene000010:CDS | 30.0% | |
| !! | TCAATTATTGATGGACTCAA+TGG | + | 33294:50782-50801 | MS.gene000010:CDS | 30.0% |
| AACGAAGGGTCTATTGAAAA+AGG | + | 33294:50215-50234 | MS.gene000010:CDS | 35.0% | |
| AAGAGACTTTCTTGACACAA+AGG | + | 33294:50400-50419 | MS.gene000010:CDS | 35.0% | |
| AGAGACTTTCTTGACACAAA+GGG | + | 33294:50401-50420 | MS.gene000010:CDS | 35.0% | |
| CATTAATAGGTATCCCAACA+TGG | - | 33294:50743-50762 | MS.gene000010:intergenic | 35.0% | |
| GAGTGAAGAAATGGCAATTA+TGG | + | 33294:50710-50729 | MS.gene000010:CDS | 35.0% | |
| GCATGACAATATGTCAACAA+TGG | + | 33294:50524-50543 | MS.gene000010:CDS | 35.0% | |
| GTTGATTGACCACAATTTGA+AGG | + | 33294:50566-50585 | MS.gene000010:CDS | 35.0% | |
| TACTAACGTCCTTCAAATTG+TGG | - | 33294:50578-50597 | MS.gene000010:intergenic | 35.0% | |
| TCATTGTCAATGATGTTGCA+AGG | + | 33294:50172-50191 | MS.gene000010:CDS | 35.0% | |
| TGCAACATCATTGACAATGA+AGG | - | 33294:50172-50191 | MS.gene000010:intergenic | 35.0% | |
| TGGATATGAGTTATCTTGCT+TGG | + | 33294:50544-50563 | MS.gene000010:CDS | 35.0% | |
| TTTGTGTCAAGAAAGTCTCT+TGG | - | 33294:50401-50420 | MS.gene000010:intergenic | 35.0% | |
| !! | ATTATTGATGGACTCAATGG+TGG | + | 33294:50785-50804 | MS.gene000010:CDS | 35.0% |
| !! | TGAATTTTGAGAGCACAGAT+TGG | - | 33294:50338-50357 | MS.gene000010:intergenic | 35.0% |
| AAACCAAGTCCAACTCGAAA+TGG | + | 33294:50650-50669 | MS.gene000010:CDS | 40.0% | |
| AGCATCTAGGAGTGAAGAAA+TGG | + | 33294:50701-50720 | MS.gene000010:CDS | 40.0% | |
| CTCATAAGGTCCATACACTA+TGG | - | 33294:50280-50299 | MS.gene000010:intergenic | 40.0% | |
| GAAATGGCAATTATGGGTCA+CGG | + | 33294:50717-50736 | MS.gene000010:CDS | 40.0% | |
| TCAATTTCTCTTCAGCCTTC+AGG | - | 33294:50428-50447 | MS.gene000010:intergenic | 40.0% | |
| TTAGTAGACGTTTGGAAGCA+TGG | + | 33294:50592-50611 | MS.gene000010:CDS | 40.0% | |
| ! | TAGTAGACGTTTGGAAGCAT+GGG | + | 33294:50593-50612 | MS.gene000010:CDS | 40.0% |
| ATGAGCCTCAACCTGAGATT+TGG | + | 33294:50295-50314 | MS.gene000010:CDS | 45.0% | |
| CCAGAATTGACCTCACCAAA+TGG | - | 33294:50834-50853 | MS.gene000010:intergenic | 45.0% | |
| GAAGGACGTTAGTAGACGTT+TGG | + | 33294:50584-50603 | MS.gene000010:CDS | 45.0% | |
| TCTGGTAAAGGCCAAATCTC+AGG | - | 33294:50309-50328 | MS.gene000010:intergenic | 45.0% | |
| ! | AAAGGCCAAATCTCAGGTTG+AGG | - | 33294:50303-50322 | MS.gene000010:intergenic | 45.0% |
| ! | AGATTTGGCCTTTACCAGAG+GGG | + | 33294:50310-50329 | MS.gene000010:CDS | 45.0% |
| ! | CCATTTGGTGAGGTCAATTC+TGG | + | 33294:50831-50850 | MS.gene000010:CDS | 45.0% |
| ! | GAGATTTGGCCTTTACCAGA+GGG | + | 33294:50309-50328 | MS.gene000010:CDS | 45.0% |
| ! | TGAGATTTGGCCTTTACCAG+AGG | + | 33294:50308-50327 | MS.gene000010:CDS | 45.0% |
| !! | ATCTCAGGTTGAGGCTCATA+AGG | - | 33294:50294-50313 | MS.gene000010:intergenic | 45.0% |
| !! | GTGAGGTCAATTCTGGAGTT+TGG | + | 33294:50838-50857 | MS.gene000010:CDS | 45.0% |
| ACAGGCTTCAATGCCACAGA+GGG | - | 33294:50256-50275 | MS.gene000010:intergenic | 50.0% | |
| AGGTCCATACACTATGGCAC+AGG | - | 33294:50274-50293 | MS.gene000010:intergenic | 50.0% | |
| ATTATGGGTCACGGCCATGT+TGG | + | 33294:50726-50745 | MS.gene000010:CDS | 50.0% | |
| CACCACTTCTAGCAGCATCT+AGG | + | 33294:50688-50707 | MS.gene000010:CDS | 50.0% | |
| CCAAGTCCAACTCGAAATGG+CGG | + | 33294:50653-50672 | MS.gene000010:CDS | 50.0% | |
| CTCCTAGATGCTGCTAGAAG+TGG | - | 33294:50693-50712 | MS.gene000010:intergenic | 50.0% | |
| CTTGACACAAAGGGTCCTGA+AGG | + | 33294:50410-50429 | MS.gene000010:CDS | 50.0% | |
| GAAGCCTGTGCCATAGTGTA+TGG | + | 33294:50267-50286 | MS.gene000010:CDS | 50.0% | |
| GATGCTGCTAGAAGTGGTGA+TGG | - | 33294:50687-50706 | MS.gene000010:intergenic | 50.0% | |
| TTATGGGTCACGGCCATGTT+GGG | + | 33294:50727-50746 | MS.gene000010:CDS | 50.0% | |
| ! | GATTTGGCCTTTACCAGAGG+GGG | + | 33294:50311-50330 | MS.gene000010:CDS | 50.0% |
| ! | GTGCAAGAGACAGTGCCATT+TGG | + | 33294:50816-50835 | MS.gene000010:CDS | 50.0% |
| ! | TTGTTGCCGCCATTTCGAGT+TGG | - | 33294:50662-50681 | MS.gene000010:intergenic | 50.0% |
| !! | AGAGACAGTGCCATTTGGTG+AGG | + | 33294:50821-50840 | MS.gene000010:CDS | 50.0% |
| !! | TTGATGGACTCAATGGTGGC+TGG | + | 33294:50789-50808 | MS.gene000010:CDS | 50.0% |
| CACAGGCTTCAATGCCACAG+AGG | - | 33294:50257-50276 | MS.gene000010:intergenic | 55.0% | |
| GATTGGACCCCCTCTGGTAA+AGG | - | 33294:50321-50340 | MS.gene000010:intergenic | 55.0% | |
| GCTGAACTTAGCACCCTCTG+TGG | + | 33294:50240-50259 | MS.gene000010:CDS | 55.0% | |
| !! | CCGCCATTTCGAGTTGGACT+TGG | - | 33294:50656-50675 | MS.gene000010:intergenic | 55.0% |
| AGCACAGATTGGACCCCCTC+TGG | - | 33294:50327-50346 | MS.gene000010:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33294 | gene | 50141 | 50878 | 50141 | ID=MS.gene000010 |
| 33294 | mRNA | 50141 | 50878 | 50141 | ID=MS.gene000010.t1;Parent=MS.gene000010 |
| 33294 | exon | 50141 | 50878 | 50141 | ID=MS.gene000010.t1.exon1;Parent=MS.gene000010.t1 |
| 33294 | CDS | 50141 | 50878 | 50141 | ID=cds.MS.gene000010.t1;Parent=MS.gene000010.t1 |
| Gene Sequence |
| Protein sequence |
