Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000011.t1 | XP_003605474.1 | 68.4 | 234 | 57 | 2 | 1 | 222 | 1 | 229 | 1.10E-77 | 299.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000011.t1 | Q9FJK3 | 52.6 | 154 | 72 | 1 | 1 | 154 | 1 | 153 | 2.2e-37 | 157.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000011.t1 | G7JM29 | 68.4 | 234 | 57 | 2 | 1 | 222 | 1 | 229 | 8.3e-78 | 299.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene000011.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000011.t1 | MTR_4g031910 | 71.368 | 234 | 50 | 2 | 1 | 222 | 1 | 229 | 3.19e-111 | 318 |
| MS.gene000011.t1 | MTR_4g032290 | 69.262 | 244 | 40 | 2 | 1 | 222 | 1 | 231 | 4.46e-108 | 310 |
| MS.gene000011.t1 | MTR_4g032620 | 56.277 | 231 | 92 | 1 | 1 | 222 | 1 | 231 | 3.28e-92 | 270 |
| MS.gene000011.t1 | MTR_8g036130 | 56.275 | 247 | 83 | 4 | 1 | 222 | 1 | 247 | 1.68e-86 | 256 |
| MS.gene000011.t1 | MTR_4g032260 | 80.255 | 157 | 31 | 0 | 1 | 157 | 1 | 157 | 3.05e-81 | 239 |
| MS.gene000011.t1 | MTR_3g065100 | 46.058 | 241 | 105 | 4 | 1 | 219 | 1 | 238 | 2.10e-59 | 187 |
| MS.gene000011.t1 | MTR_3g466980 | 54.545 | 154 | 69 | 1 | 1 | 154 | 1 | 153 | 1.61e-57 | 181 |
| MS.gene000011.t1 | MTR_2g035610 | 70.492 | 122 | 36 | 0 | 1 | 122 | 1 | 122 | 1.43e-49 | 158 |
| MS.gene000011.t1 | MTR_5g075380 | 45.806 | 155 | 83 | 1 | 1 | 155 | 1 | 154 | 4.20e-43 | 143 |
| MS.gene000011.t1 | MTR_3g466830 | 46.358 | 151 | 80 | 1 | 3 | 153 | 2 | 151 | 1.11e-42 | 142 |
| MS.gene000011.t1 | MTR_4g063790 | 38.889 | 180 | 96 | 1 | 1 | 180 | 1 | 166 | 3.59e-42 | 140 |
| MS.gene000011.t1 | MTR_4g032270 | 53.285 | 137 | 49 | 2 | 94 | 222 | 2 | 131 | 1.44e-41 | 138 |
| MS.gene000011.t1 | MTR_3g467080 | 40.385 | 156 | 93 | 0 | 1 | 156 | 1 | 156 | 8.85e-40 | 134 |
| MS.gene000011.t1 | MTR_5g047580 | 44.231 | 156 | 85 | 2 | 1 | 156 | 1 | 154 | 4.09e-39 | 132 |
| MS.gene000011.t1 | MTR_3g466890 | 47.619 | 126 | 66 | 0 | 3 | 128 | 2 | 127 | 1.56e-37 | 129 |
| MS.gene000011.t1 | MTR_1g077390 | 41.026 | 156 | 90 | 2 | 1 | 156 | 1 | 154 | 1.30e-36 | 126 |
| MS.gene000011.t1 | MTR_3g031100 | 36.571 | 175 | 110 | 1 | 1 | 175 | 1 | 174 | 3.28e-36 | 127 |
| MS.gene000011.t1 | MTR_2g016210 | 37.209 | 172 | 107 | 1 | 1 | 171 | 1 | 172 | 5.58e-36 | 127 |
| MS.gene000011.t1 | MTR_1g090710 | 38.961 | 154 | 93 | 1 | 1 | 154 | 1 | 153 | 8.71e-34 | 119 |
| MS.gene000011.t1 | MTR_1g077320 | 43.590 | 156 | 86 | 2 | 1 | 156 | 1 | 154 | 3.43e-33 | 117 |
| MS.gene000011.t1 | MTR_7g011950 | 42.949 | 156 | 87 | 2 | 1 | 156 | 1 | 154 | 9.34e-33 | 116 |
| MS.gene000011.t1 | MTR_3g465410 | 38.158 | 152 | 80 | 1 | 29 | 180 | 1 | 138 | 1.74e-32 | 115 |
| MS.gene000011.t1 | MTR_1g090783 | 38.312 | 154 | 93 | 2 | 3 | 156 | 2 | 153 | 3.26e-32 | 115 |
| MS.gene000011.t1 | MTR_4g028720 | 42.308 | 156 | 88 | 2 | 1 | 156 | 1 | 154 | 9.55e-32 | 114 |
| MS.gene000011.t1 | MTR_1g090697 | 40.000 | 150 | 88 | 2 | 1 | 150 | 1 | 148 | 1.05e-31 | 113 |
| MS.gene000011.t1 | MTR_1g077300 | 42.949 | 156 | 85 | 2 | 1 | 156 | 1 | 152 | 1.34e-31 | 113 |
| MS.gene000011.t1 | MTR_1g084950 | 42.949 | 156 | 87 | 2 | 1 | 156 | 1 | 154 | 2.66e-31 | 112 |
| MS.gene000011.t1 | MTR_4g028800 | 74.286 | 70 | 16 | 1 | 1 | 70 | 1 | 68 | 2.37e-30 | 107 |
| MS.gene000011.t1 | MTR_7g055800 | 49.573 | 117 | 31 | 2 | 1 | 117 | 1 | 89 | 1.13e-28 | 103 |
| MS.gene000011.t1 | MTR_3g031240 | 38.938 | 113 | 69 | 0 | 1 | 113 | 1 | 113 | 2.77e-27 | 103 |
| MS.gene000011.t1 | MTR_7g055790 | 52.252 | 111 | 35 | 1 | 1 | 111 | 59 | 151 | 5.33e-26 | 99.0 |
| MS.gene000011.t1 | MTR_3g466900 | 39.024 | 123 | 74 | 1 | 32 | 154 | 2 | 123 | 6.11e-26 | 98.2 |
| MS.gene000011.t1 | MTR_3g466930 | 43.333 | 90 | 51 | 0 | 32 | 121 | 2 | 91 | 4.55e-24 | 93.2 |
| MS.gene000011.t1 | MTR_7g106510 | 39.370 | 127 | 77 | 0 | 3 | 129 | 6 | 132 | 4.60e-24 | 99.8 |
| MS.gene000011.t1 | MTR_4g019670 | 40.157 | 127 | 76 | 0 | 3 | 129 | 6 | 132 | 5.65e-24 | 99.4 |
| MS.gene000011.t1 | MTR_2g035580 | 35.032 | 157 | 101 | 1 | 1 | 157 | 1 | 156 | 2.88e-23 | 92.0 |
| MS.gene000011.t1 | MTR_7g055940 | 48.113 | 106 | 27 | 2 | 1 | 106 | 1 | 78 | 4.00e-23 | 89.0 |
| MS.gene000011.t1 | MTR_5g047560 | 41.026 | 117 | 52 | 2 | 1 | 117 | 1 | 100 | 7.48e-22 | 87.8 |
| MS.gene000011.t1 | MTR_3g093900 | 34.646 | 127 | 83 | 0 | 3 | 129 | 6 | 132 | 6.47e-21 | 90.9 |
| MS.gene000011.t1 | MTR_2g035590 | 60.494 | 81 | 32 | 0 | 74 | 154 | 1 | 81 | 2.94e-19 | 79.7 |
| MS.gene000011.t1 | MTR_1g114730 | 30.088 | 113 | 72 | 3 | 1 | 107 | 1 | 112 | 1.55e-12 | 65.9 |
| MS.gene000011.t1 | MTR_6g018920 | 31.858 | 113 | 70 | 4 | 1 | 107 | 1 | 112 | 1.57e-12 | 65.9 |
| MS.gene000011.t1 | MTR_6g005450 | 33.333 | 114 | 67 | 4 | 1 | 107 | 1 | 112 | 2.48e-12 | 65.5 |
| MS.gene000011.t1 | MTR_4g084780 | 36.937 | 111 | 54 | 2 | 74 | 168 | 1 | 111 | 4.71e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000011.t1 | AT5G48670 | 52.597 | 154 | 72 | 1 | 1 | 154 | 1 | 153 | 1.82e-43 | 149 |
| MS.gene000011.t1 | AT1G65330 | 43.452 | 168 | 85 | 2 | 3 | 161 | 2 | 168 | 1.10e-39 | 137 |
| MS.gene000011.t1 | AT1G65300 | 44.079 | 152 | 84 | 1 | 3 | 154 | 2 | 152 | 4.94e-39 | 136 |
| MS.gene000011.t1 | AT5G26630 | 42.365 | 203 | 106 | 2 | 1 | 193 | 1 | 202 | 4.49e-38 | 132 |
| MS.gene000011.t1 | AT5G27960 | 41.667 | 168 | 87 | 3 | 5 | 162 | 3 | 169 | 6.61e-37 | 131 |
| MS.gene000011.t1 | AT5G26650 | 37.981 | 208 | 110 | 5 | 5 | 202 | 3 | 201 | 6.83e-37 | 132 |
| MS.gene000011.t1 | AT1G31630 | 35.714 | 168 | 97 | 3 | 3 | 161 | 2 | 167 | 1.12e-32 | 121 |
| MS.gene000011.t1 | AT1G31640 | 38.411 | 151 | 91 | 2 | 3 | 153 | 2 | 150 | 6.75e-31 | 118 |
| MS.gene000011.t1 | AT2G28700 | 36.364 | 176 | 107 | 2 | 1 | 174 | 1 | 173 | 1.76e-30 | 115 |
| MS.gene000011.t1 | AT5G27810 | 53.763 | 93 | 43 | 0 | 29 | 121 | 1 | 93 | 2.05e-30 | 109 |
| MS.gene000011.t1 | AT3G05860 | 39.669 | 121 | 73 | 0 | 1 | 121 | 1 | 121 | 2.14e-28 | 106 |
| MS.gene000011.t1 | AT3G05860 | 39.669 | 121 | 73 | 0 | 1 | 121 | 1 | 121 | 6.05e-28 | 106 |
| MS.gene000011.t1 | AT3G05860 | 39.669 | 121 | 73 | 0 | 1 | 121 | 1 | 121 | 7.73e-28 | 106 |
| MS.gene000011.t1 | AT5G26580 | 34.884 | 172 | 85 | 5 | 1 | 161 | 1 | 156 | 6.24e-25 | 100 |
| MS.gene000011.t1 | AT1G22590 | 30.864 | 162 | 108 | 1 | 1 | 162 | 1 | 158 | 3.09e-24 | 94.7 |
| MS.gene000011.t1 | AT5G06500 | 33.758 | 157 | 102 | 2 | 1 | 157 | 1 | 155 | 4.11e-20 | 85.9 |
| MS.gene000011.t1 | AT2G40210 | 40.310 | 129 | 76 | 1 | 1 | 129 | 1 | 128 | 9.63e-20 | 86.7 |
| MS.gene000011.t1 | AT5G58890 | 37.795 | 127 | 68 | 5 | 6 | 123 | 6 | 130 | 2.98e-15 | 73.6 |
| MS.gene000011.t1 | AT5G55690 | 41.026 | 78 | 39 | 2 | 1 | 71 | 1 | 78 | 1.35e-12 | 65.9 |
| MS.gene000011.t1 | AT5G55690 | 41.026 | 78 | 39 | 2 | 1 | 71 | 1 | 78 | 1.35e-12 | 65.9 |
Find 0 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAACAAAGAAAAGAAAAT+AGG | - | 33308:17913-17932 | MS.gene000011:CDS | 20.0% |
| !! | GAAACAAAGAAAAGAAAATA+GGG | - | 33308:17914-17933 | MS.gene000011:CDS | 20.0% |
| ! | ATCATACAAGAAAAGAAGAA+AGG | - | 33308:17665-17684 | MS.gene000011:CDS | 25.0% |
| ! | TCAATTATTGATAGACTCAA+TGG | - | 33308:18181-18200 | MS.gene000011:CDS | 25.0% |
| ! | TCATACAAGAAAAGAAGAAA+GGG | - | 33308:17666-17685 | MS.gene000011:CDS | 25.0% |
| !! | TAGATTTGAGTTATCTTACT+TGG | - | 33308:18009-18028 | MS.gene000011:CDS | 25.0% |
| AAGAAAGGGTCTATTGAAAA+AGG | - | 33308:17680-17699 | MS.gene000011:CDS | 30.0% | |
| ATTATTGATAGACTCAATGG+TGG | - | 33308:18184-18203 | MS.gene000011:CDS | 30.0% | |
| CATTAATAGATATGCCAACA+TGG | + | 33308:18142-18161 | MS.gene000011:intergenic | 30.0% | |
| GTTGAATGACCATAATTTGA+AGG | - | 33308:18031-18050 | MS.gene000011:CDS | 30.0% | |
| TACCAATGTCCTTCAAATTA+TGG | + | 33308:18043-18062 | MS.gene000011:intergenic | 30.0% | |
| AAGAGACTTTCTTGACACAA+AGG | - | 33308:17865-17884 | MS.gene000011:CDS | 35.0% | |
| AGAGACTTTCTTGACACAAA+GGG | - | 33308:17866-17885 | MS.gene000011:CDS | 35.0% | |
| ATGTCTCATTGTCTCAATGA+AGG | - | 33308:17957-17976 | MS.gene000011:CDS | 35.0% | |
| ATTATGGATCATAGCCATGT+TGG | - | 33308:18125-18144 | MS.gene000011:CDS | 35.0% | |
| CTTGTATGATTCTTTCCTTG+CGG | + | 33308:17655-17674 | MS.gene000011:intergenic | 35.0% | |
| GACCATAATTTGAAGGACAT+TGG | - | 33308:18038-18057 | MS.gene000011:CDS | 35.0% | |
| GAGTGAAGAAATGGCAATTA+TGG | - | 33308:18109-18128 | MS.gene000011:CDS | 35.0% | |
| TTTGTGTCAAGAAAGTCTCT+TGG | + | 33308:17866-17885 | MS.gene000011:intergenic | 35.0% | |
| AGCATCTAGGAGTGAAGAAA+TGG | - | 33308:18100-18119 | MS.gene000011:CDS | 40.0% | |
| CCAGAATTGATCTCACCAAA+GGG | + | 33308:18233-18252 | MS.gene000011:intergenic | 40.0% | |
| TCCAGAATTGATCTCACCAA+AGG | + | 33308:18234-18253 | MS.gene000011:intergenic | 40.0% | |
| TGTGCACGACAATATGTCAA+CGG | - | 33308:17986-18005 | MS.gene000011:CDS | 40.0% | |
| !! | CGTCATGAATTTTGAGAGCA+CGG | + | 33308:17808-17827 | MS.gene000011:intergenic | 40.0% |
| !! | TGAATTTTGAGAGCACGGAT+TGG | + | 33308:17803-17822 | MS.gene000011:intergenic | 40.0% |
| CCCTTTGGTGAGATCAATTC+TGG | - | 33308:18230-18249 | MS.gene000011:CDS | 45.0% | |
| CTCATAAGGGCCATACACTA+TGG | + | 33308:17745-17764 | MS.gene000011:intergenic | 45.0% | |
| GAAGGACATTGGTAGACGTT+TGG | - | 33308:18049-18068 | MS.gene000011:CDS | 45.0% | |
| TCATTGTCAATGATGCCGCA+AGG | - | 33308:17637-17656 | MS.gene000011:CDS | 45.0% | |
| TGCGGCATCATTGACAATGA+AGG | + | 33308:17637-17656 | MS.gene000011:intergenic | 45.0% | |
| TTGATAGACTCAATGGTGGC+TGG | - | 33308:18188-18207 | MS.gene000011:CDS | 45.0% | |
| ! | CTTGACACAAAGGGTTCTGA+AGG | - | 33308:17875-17894 | MS.gene000011:CDS | 45.0% |
| ACAGGCTTCAATGCCACAGA+GGG | + | 33308:17721-17740 | MS.gene000011:intergenic | 50.0% | |
| GAAGCCTGTGCCATAGTGTA+TGG | - | 33308:17732-17751 | MS.gene000011:CDS | 50.0% | |
| GCTGAACTTAACACCCTCTG+TGG | - | 33308:17705-17724 | MS.gene000011:CDS | 50.0% | |
| GGACATTGGTAGACGTTTGG+CGG | - | 33308:18052-18071 | MS.gene000011:CDS | 50.0% | |
| TCTGGTGAAGGCCAAATCTC+TGG | + | 33308:17774-17793 | MS.gene000011:intergenic | 50.0% | |
| TCTTCACTCCTAGATGCTGC+TGG | + | 33308:18098-18117 | MS.gene000011:intergenic | 50.0% | |
| ! | AGAGATTTGGCCTTCACCAG+AGG | - | 33308:17773-17792 | MS.gene000011:CDS | 50.0% |
| ! | AGATTTGGCCTTCACCAGAG+GGG | - | 33308:17775-17794 | MS.gene000011:CDS | 50.0% |
| ! | GAGATTTGGCCTTCACCAGA+GGG | - | 33308:17774-17793 | MS.gene000011:CDS | 50.0% |
| AGGGCCATACACTATGGCAC+AGG | + | 33308:17739-17758 | MS.gene000011:intergenic | 55.0% | |
| ATCTCTGGCGGAGGCTCATA+AGG | + | 33308:17759-17778 | MS.gene000011:intergenic | 55.0% | |
| ATGAGCCTCCGCCAGAGATT+TGG | - | 33308:17760-17779 | MS.gene000011:CDS | 55.0% | |
| CACAGGCTTCAATGCCACAG+AGG | + | 33308:17722-17741 | MS.gene000011:intergenic | 55.0% | |
| GGTGAAGGCCAAATCTCTGG+CGG | + | 33308:17771-17790 | MS.gene000011:intergenic | 55.0% | |
| TCACTCCTAGATGCTGCTGG+AGG | + | 33308:18095-18114 | MS.gene000011:intergenic | 55.0% | |
| TCTCTGGCGGAGGCTCATAA+GGG | + | 33308:17758-17777 | MS.gene000011:intergenic | 55.0% | |
| ! | GATTTGGCCTTCACCAGAGG+GGG | - | 33308:17776-17795 | MS.gene000011:CDS | 55.0% |
| CACCACCTCCAGCAGCATCT+AGG | - | 33308:18087-18106 | MS.gene000011:CDS | 60.0% | |
| CTAGATGCTGCTGGAGGTGG+TGG | + | 33308:18089-18108 | MS.gene000011:intergenic | 60.0% | |
| CTCCTAGATGCTGCTGGAGG+TGG | + | 33308:18092-18111 | MS.gene000011:intergenic | 60.0% | |
| GAAGGCCAAATCTCTGGCGG+AGG | + | 33308:17768-17787 | MS.gene000011:intergenic | 60.0% | |
| GATTGGACCCCCTCTGGTGA+AGG | + | 33308:17786-17805 | MS.gene000011:intergenic | 60.0% | |
| GCGCAAGAGACAGTGCCCTT+TGG | - | 33308:18215-18234 | MS.gene000011:CDS | 60.0% | |
| ! | GATGCTGCTGGAGGTGGTGG+TGG | + | 33308:18086-18105 | MS.gene000011:intergenic | 65.0% |
| !! | AGCACGGATTGGACCCCCTC+TGG | + | 33308:17792-17811 | MS.gene000011:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33308 | gene | 17609 | 18277 | 17609 | ID=MS.gene000011 |
| 33308 | mRNA | 17609 | 18277 | 17609 | ID=MS.gene000011.t1;Parent=MS.gene000011 |
| 33308 | exon | 17609 | 18277 | 17609 | ID=MS.gene000011.t1.exon1;Parent=MS.gene000011.t1 |
| 33308 | CDS | 17609 | 18277 | 17609 | ID=cds.MS.gene000011.t1;Parent=MS.gene000011.t1 |
| Gene Sequence |
| Protein sequence |
