AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00003

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00003.t1 MTR_2g014680 93.506 308 19 1 1 307 1 308 0.0 605
MS.gene00003.t1 MTR_4g088245 62.154 325 87 9 1 301 1 313 9.83e-137 390
MS.gene00003.t1 MTR_8g069160 69.507 223 53 3 1 208 1 223 4.12e-114 330
MS.gene00003.t1 MTR_4g078875 60.662 272 100 6 12 278 5 274 1.23e-110 322
MS.gene00003.t1 MTR_2g090735 58.219 292 108 6 1 289 1 281 1.29e-105 310
MS.gene00003.t1 MTR_8g102240 61.233 227 74 5 8 224 3 225 1.35e-87 270
MS.gene00003.t1 MTR_8g102240 61.233 227 74 5 8 224 3 225 1.38e-87 270
MS.gene00003.t1 MTR_5g053430 59.278 194 76 2 27 220 34 224 2.99e-74 234
MS.gene00003.t1 MTR_5g053430 59.278 194 76 2 27 220 34 224 3.93e-74 234
MS.gene00003.t1 MTR_1g093680 50.000 248 118 4 2 246 7 251 1.74e-73 234
MS.gene00003.t1 MTR_7g033320 56.757 185 73 3 31 212 35 215 1.52e-67 219
MS.gene00003.t1 MTR_1g093670 46.798 203 87 3 32 233 7 189 8.57e-50 169
MS.gene00003.t1 MTR_4g078895 60.000 65 24 1 30 94 16 78 1.24e-19 82.0
MS.gene00003.t1 MTR_2g014700 68.889 45 14 0 118 162 44 88 4.37e-16 72.8
MS.gene00003.t1 MTR_6g084430 32.317 164 92 6 53 213 5 152 2.22e-15 75.9
MS.gene00003.t1 MTR_7g011130 30.457 197 120 4 36 231 2 182 8.18e-15 74.3
MS.gene00003.t1 MTR_3g070030 30.899 178 99 6 41 210 7 168 1.31e-14 73.9
MS.gene00003.t1 MTR_3g109340 31.285 179 100 7 38 210 8 169 1.47e-13 70.9
MS.gene00003.t1 MTR_3g109340 31.461 178 100 7 38 210 8 168 2.03e-13 70.5
MS.gene00003.t1 MTR_3g435150 33.803 142 77 5 53 193 16 141 7.40e-13 68.2
MS.gene00003.t1 MTR_7g085260 31.111 180 93 9 46 212 10 171 1.19e-12 68.2
MS.gene00003.t1 MTR_6g032770 33.721 172 79 10 53 210 18 168 1.32e-12 68.2
MS.gene00003.t1 MTR_5g012080 26.244 221 135 8 47 259 1 201 2.26e-12 67.0
MS.gene00003.t1 MTR_1g069805 29.630 189 104 8 52 233 9 175 2.89e-12 66.2
MS.gene00003.t1 MTR_7g005280 32.667 150 73 8 53 196 15 142 3.33e-12 65.9
MS.gene00003.t1 MTR_3g022390 90.323 31 3 0 198 228 43 73 3.77e-12 61.2
MS.gene00003.t1 MTR_7g097090 30.061 163 95 6 52 211 9 155 2.06e-11 63.9
MS.gene00003.t1 MTR_8g023880 33.974 156 74 8 52 192 6 147 2.17e-11 63.5
MS.gene00003.t1 MTR_4g101680 27.647 170 101 6 47 210 1 154 2.83e-11 63.9
MS.gene00003.t1 MTR_2g078700 34.722 144 75 7 53 193 23 150 5.19e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00003.t1 AT4G28500 64.629 229 74 3 1 222 1 229 3.21e-107 314
MS.gene00003.t1 AT1G28470 56.537 283 91 6 1 252 1 282 1.23e-105 311
MS.gene00003.t1 AT4G29230 62.332 223 69 3 6 217 2 220 4.58e-92 280
MS.gene00003.t1 AT4G29230 62.332 223 69 3 6 217 2 220 1.62e-91 281
MS.gene00003.t1 AT4G29230 61.435 223 70 3 6 217 2 219 2.44e-90 278
MS.gene00003.t1 AT5G56620 62.944 197 59 4 32 217 25 218 2.99e-81 251
MS.gene00003.t1 AT5G56620 62.944 197 59 4 32 217 25 218 3.21e-81 252
MS.gene00003.t1 AT5G56620 60.476 210 69 4 19 217 12 218 5.32e-81 250
MS.gene00003.t1 AT1G25580 56.545 191 74 4 27 217 34 215 9.60e-69 221
MS.gene00003.t1 AT5G14490 50.244 205 97 4 25 228 29 229 3.31e-57 186
MS.gene00003.t1 AT5G14490 51.000 200 93 4 30 228 37 232 8.72e-57 187
MS.gene00003.t1 AT5G14490 51.000 200 93 4 30 228 34 229 9.07e-57 187
MS.gene00003.t1 AT5G14490 51.000 200 93 4 30 228 37 232 1.17e-56 187
MS.gene00003.t1 AT3G01600 50.000 186 89 3 30 214 34 216 2.68e-51 173
MS.gene00003.t1 AT3G04060 32.609 184 98 7 41 215 8 174 1.36e-18 85.1
MS.gene00003.t1 AT5G18270 33.514 185 94 10 41 215 10 175 7.62e-16 77.4
MS.gene00003.t1 AT5G18270 33.514 185 94 10 41 215 10 175 1.41e-15 76.6
MS.gene00003.t1 AT3G18400 32.530 166 84 7 53 210 5 150 4.88e-15 74.7
MS.gene00003.t1 AT1G33060 30.645 186 94 9 40 212 11 174 8.64e-14 72.0
MS.gene00003.t1 AT1G33060 30.645 186 94 9 40 212 11 174 8.82e-14 72.0
MS.gene00003.t1 AT4G35580 34.416 154 74 8 46 192 2 135 1.10e-12 68.6
MS.gene00003.t1 AT4G35580 34.416 154 74 8 46 192 2 135 1.12e-12 68.6
MS.gene00003.t1 AT4G35580 34.416 154 74 8 46 192 2 135 1.17e-12 68.6
MS.gene00003.t1 AT2G24430 29.586 169 97 6 52 214 15 167 1.20e-12 67.8
MS.gene00003.t1 AT2G24430 29.586 169 97 6 52 214 15 167 1.20e-12 67.8
MS.gene00003.t1 AT1G76420 29.279 222 128 11 52 265 21 221 7.29e-12 65.5
MS.gene00003.t1 AT1G01720 30.464 151 83 6 47 194 1 132 1.93e-11 63.9
MS.gene00003.t1 AT2G33480 29.412 187 98 10 41 221 7 165 2.88e-11 63.2
MS.gene00003.t1 AT3G29035 32.867 143 77 5 53 193 24 149 2.89e-11 63.5
MS.gene00003.t1 AT2G33480 29.101 189 98 10 41 221 7 167 3.83e-11 62.8
MS.gene00003.t1 AT1G12260 29.825 171 96 8 47 210 1 154 4.22e-11 63.5
MS.gene00003.t1 AT1G12260 30.769 156 86 7 47 197 1 139 5.53e-11 63.2

Find 56 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAACAAGATGGCACAAAAC+TGG 0.293534 2.4:+70168429 MS.gene00003:CDS
TCTAGTACTTCTACATCATT+TGG 0.314489 2.4:-70166830 None:intergenic
TCCACAAGAGCTACATTGTT+GGG 0.338920 2.4:-70168735 None:intergenic
CCAACGCTTCAAGGAGATAA+TGG 0.355602 2.4:+70166917 MS.gene00003:CDS
ATCACCCAGTTAGTCTTCTC+TGG 0.385881 2.4:-70168540 None:intergenic
TGTAGAAGTACTAGAGCATT+TGG 0.390413 2.4:+70166838 MS.gene00003:CDS
GAAAACCAGAGAAGACTAAC+TGG 0.406375 2.4:+70168535 MS.gene00003:CDS
AACTATTATTCGCTTATCAA+TGG 0.414728 2.4:+70168863 MS.gene00003:CDS
GGGTGGAGTTAATGAATTGC+CGG 0.417191 2.4:+70166781 MS.gene00003:intron
CATGTTGTCTAGGCTGTGTC+TGG 0.424066 2.4:-70168632 None:intergenic
TCACTTTGAACAGACATGTT+TGG 0.426034 2.4:-70168837 None:intergenic
CTAGTACTTCTACATCATTT+GGG 0.458512 2.4:-70166829 None:intergenic
GAGTTCATACCAACGCTTCA+AGG 0.459148 2.4:+70166908 MS.gene00003:CDS
CCATTATCTCCTTGAAGCGT+TGG 0.461132 2.4:-70166917 None:intergenic
GGGTCAAATTTCACTCCAGC+TGG 0.465906 2.4:-70166809 None:intergenic
GGGCTGCACTGTTATTATCA+TGG 0.468166 2.4:-70168713 None:intergenic
ATATGCATGTTGGTCATAGT+AGG 0.471621 2.4:+70168790 MS.gene00003:CDS
GCCGCATGAGGGGCATTCAA+TGG 0.476343 2.4:-70166486 None:intergenic
TACACCAACTATTGCAAAGA+AGG 0.482059 2.4:+70168513 MS.gene00003:CDS
CCTTCAAAAGCATACACAAC+AGG 0.484981 2.4:+70168363 MS.gene00003:CDS
CTTCTTCATTCGTACCAAGA+TGG 0.492058 2.4:-70168572 None:intergenic
TACTATGACCAACATGCATA+TGG 0.492828 2.4:-70168788 None:intergenic
TGCAAACAAAGTAAGAGTAC+TGG 0.494119 2.4:+70168890 MS.gene00003:CDS
GAATTGCCGGGGTTACCAGC+TGG 0.498475 2.4:+70166794 MS.gene00003:CDS
GGTGGAGTTAATGAATTGCC+GGG 0.501114 2.4:+70166782 MS.gene00003:CDS
AAAATAATCGACAAAGGAAG+TGG 0.506740 2.4:+70168690 MS.gene00003:CDS
TTCAATATTATGGCCGCATG+AGG 0.506854 2.4:-70166498 None:intergenic
AAATCCCTTCACAACACCAT+TGG 0.513981 2.4:-70168472 None:intergenic
AACATGTCTGTTCAAAGTGA+TGG 0.516868 2.4:+70168840 MS.gene00003:CDS
TTCCACAAGAGCTACATTGT+TGG 0.516933 2.4:-70168736 None:intergenic
TCAATATTATGGCCGCATGA+GGG 0.518397 2.4:-70166497 None:intergenic
GTTGCAGGAGTTAGCAAAGA+TGG 0.520564 2.4:+70168315 MS.gene00003:intron
ATGGAATTACCATGTTGTCT+AGG 0.524147 2.4:-70168642 None:intergenic
CCCCAACAATGTAGCTCTTG+TGG 0.528333 2.4:+70168734 MS.gene00003:CDS
AAGAAGGAATTGAAACAAGA+TGG 0.540463 2.4:+70168418 MS.gene00003:CDS
GTGATGCATCAATACCATCT+TGG 0.547087 2.4:+70168558 MS.gene00003:CDS
AAACAAGATGGCACAAAACT+GGG 0.551473 2.4:+70168430 MS.gene00003:CDS
TTCACTCCAGCTGGTAACCC+CGG 0.559582 2.4:-70166800 None:intergenic
AAGGTTCACACTGATGAAGA+AGG 0.564034 2.4:+70168402 MS.gene00003:CDS
CAATATTATGGCCGCATGAG+GGG 0.567135 2.4:-70166496 None:intergenic
AGGAACAAGGAAAAGAAGAA+AGG 0.568088 2.4:+70168383 MS.gene00003:CDS
ACGAATGAAGAAGAAAGAGA+TGG 0.571736 2.4:+70168582 MS.gene00003:CDS
ATGTTTGGTATAATCAGTTG+TGG 0.571913 2.4:-70168822 None:intergenic
TATGCATGTTGGTCATAGTA+GGG 0.574413 2.4:+70168791 MS.gene00003:CDS
CCACAAGAGCTACATTGTTG+GGG 0.578626 2.4:-70168734 None:intergenic
ACACACCCAGCACAACTACC+AGG 0.587266 2.4:+70166950 MS.gene00003:CDS
GGTATGAACTCATCAATAAG+TGG 0.587548 2.4:-70166896 None:intergenic
CAGACACAGCCTAGACAACA+TGG 0.593480 2.4:+70168633 MS.gene00003:CDS
AATTATGACCATATGCATGT+TGG 0.599378 2.4:+70168780 MS.gene00003:CDS
ACCATTGAATGCCCCTCATG+CGG 0.616642 2.4:+70166485 MS.gene00003:CDS
AGAAGTACTAGAGCATTTGG+AGG 0.620573 2.4:+70166841 MS.gene00003:CDS
AAAGATTAAGAACTATGGCA+TGG 0.622011 2.4:+70166387 None:intergenic
AAGCATACACAACAGGAACA+AGG 0.626885 2.4:+70168370 MS.gene00003:CDS
CACAAGAGCTACATTGTTGG+GGG 0.638019 2.4:-70168733 None:intergenic
GTGGAGTTAATGAATTGCCG+GGG 0.680068 2.4:+70166783 MS.gene00003:CDS
AAAACCAGAGAAGACTAACT+GGG 0.696934 2.4:+70168536 MS.gene00003:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTATCATAAGTTAATAAAA+AGG - chr2.4:70167676-70167695 None:intergenic 10.0%
!! TTATCATAAGTTAATAAAAA+GGG - chr2.4:70167675-70167694 None:intergenic 10.0%
!!! AAAAAAGTGATTTTTTTTTA+AGG + chr2.4:70167543-70167562 MS.gene00003:intron 10.0%
!!! AAAAAGTGATTTTTTTTTAA+GGG + chr2.4:70167544-70167563 MS.gene00003:intron 10.0%
!!! TGAAATAGATTTTAATTATA+AGG + chr2.4:70167152-70167171 MS.gene00003:intron 10.0%
!!! TTTTTAAAAATGGATTTTTA+TGG - chr2.4:70167368-70167387 None:intergenic 10.0%
!! AAAGAAAATTATTTGTCAAT+CGG + chr2.4:70167632-70167651 MS.gene00003:intron 15.0%
!! ATTAACATAATTTAGAGATT+TGG + chr2.4:70167002-70167021 MS.gene00003:intron 15.0%
!! TAATATTGAAGTAAAAAATC+AGG + chr2.4:70166511-70166530 MS.gene00003:CDS 15.0%
!! TTAAATTAAATGTAAAAACC+TGG - chr2.4:70166971-70166990 None:intergenic 15.0%
!!! AATAGATTTTAATTATAAGG+CGG + chr2.4:70167155-70167174 MS.gene00003:intron 15.0%
!!! CATTAACTTTCTTTTTATTA+TGG - chr2.4:70167722-70167741 None:intergenic 15.0%
!!! CTTTTTTTTTTTTTTGTGTT+GGG + chr2.4:70168061-70168080 MS.gene00003:intron 15.0%
!!! GAACTCATTTTTTTTAAAAA+TGG - chr2.4:70167378-70167397 None:intergenic 15.0%
!!! GATTTTTTACTTCAATATTA+TGG - chr2.4:70166511-70166530 None:intergenic 15.0%
!!! TAATTATTTTTTGTGAATTG+TGG + chr2.4:70167692-70167711 MS.gene00003:intron 15.0%
!! AAATTTATGAGATCTAAATG+TGG - chr2.4:70167212-70167231 None:intergenic 20.0%
!! AAGAAAATTATTTGTCAATC+GGG + chr2.4:70167633-70167652 MS.gene00003:intron 20.0%
!! CACACAAATTAATAAACTAA+AGG - chr2.4:70166736-70166755 None:intergenic 20.0%
!! CCAACACAAAAAAAAAAAAA+AGG - chr2.4:70168063-70168082 None:intergenic 20.0%
!! GTGATACATTATTAATCAAA+AGG - chr2.4:70168132-70168151 None:intergenic 20.0%
!! TAAACTAAAGGAGTTAATAT+TGG - chr2.4:70166724-70166743 None:intergenic 20.0%
!! TATGAAAAAATAATCGACAA+AGG + chr2.4:70168684-70168703 MS.gene00003:CDS 20.0%
!!! AAAAATTGTTCCTTAAGTTT+TGG + chr2.4:70168026-70168045 MS.gene00003:intron 20.0%
!!! ATCTATTTTTTTCAACTACA+AGG - chr2.4:70167422-70167441 None:intergenic 20.0%
!!! CCTTTTTTTTTTTTTTGTGT+TGG + chr2.4:70168060-70168079 MS.gene00003:intron 20.0%
!!! TAATTTGTTACTGTAACATT+AGG + chr2.4:70167975-70167994 MS.gene00003:intron 20.0%
!!! TCATTTTTAAGTGTTTGTTT+AGG - chr2.4:70167524-70167543 None:intergenic 20.0%
!!! TGAAAAAAATCTGAAACAAA+CGG + chr2.4:70167584-70167603 MS.gene00003:intron 20.0%
!!! TTGATGATTTTTGATACATA+GGG + chr2.4:70166761-70166780 MS.gene00003:intron 20.0%
!!! TTTGATGATTTTTGATACAT+AGG + chr2.4:70166760-70166779 MS.gene00003:intron 20.0%
! AAAAATCAGGTACTAATCAA+TGG + chr2.4:70166524-70166543 MS.gene00003:intron 25.0%
! AACTATTATTCGCTTATCAA+TGG + chr2.4:70168863-70168882 MS.gene00003:CDS 25.0%
! AGAAAATTATTTGTCAATCG+GGG + chr2.4:70167634-70167653 MS.gene00003:intron 25.0%
! ATAGAAGTAGTTCTTTACTT+AGG + chr2.4:70167232-70167251 MS.gene00003:intron 25.0%
! ATATGTCAAATTAGAGATGA+AGG + chr2.4:70168226-70168245 MS.gene00003:intron 25.0%
! CATTGCATGTGTTAATTATT+GGG + chr2.4:70167039-70167058 MS.gene00003:intron 25.0%
! GTACCAAAAGAAATAGAATT+GGG - chr2.4:70168154-70168173 None:intergenic 25.0%
! GTTACAGTAACAAATTACTT+AGG - chr2.4:70167972-70167991 None:intergenic 25.0%
! TCCTAAAATGAAAAGCTATT+AGG - chr2.4:70167461-70167480 None:intergenic 25.0%
! TGTACCAAAAGAAATAGAAT+TGG - chr2.4:70168155-70168174 None:intergenic 25.0%
!! GAAAAAAATCTGAAACAAAC+GGG + chr2.4:70167585-70167604 MS.gene00003:intron 25.0%
!! GTTAATTAATTTGATGCATG+CGG - chr2.4:70167097-70167116 None:intergenic 25.0%
!! TCCTAATAGCTTTTCATTTT+AGG + chr2.4:70167457-70167476 MS.gene00003:intron 25.0%
!!! AATGGATTTTTATGGATAGT+AGG - chr2.4:70167360-70167379 None:intergenic 25.0%
!!! GACACTTATTTTAAAAGAGA+CGG + chr2.4:70168255-70168274 MS.gene00003:intron 25.0%
!!! TCAAATTGCTATTGATGTTT+TGG - chr2.4:70167942-70167961 None:intergenic 25.0%
AAAATAATCGACAAAGGAAG+TGG + chr2.4:70168690-70168709 MS.gene00003:CDS 30.0%
AAACCAATGAATGCTTGAAT+TGG + chr2.4:70167810-70167829 MS.gene00003:intron 30.0%
AAGAAGGAATTGAAACAAGA+TGG + chr2.4:70168418-70168437 MS.gene00003:CDS 30.0%
AATTATGACCATATGCATGT+TGG + chr2.4:70168780-70168799 MS.gene00003:CDS 30.0%
ATGTTTGGTATAATCAGTTG+TGG - chr2.4:70168825-70168844 None:intergenic 30.0%
ATTGTTACCTAAAATGTTGC+AGG + chr2.4:70168300-70168319 MS.gene00003:intron 30.0%
CTAGTACTTCTACATCATTT+GGG - chr2.4:70166832-70166851 None:intergenic 30.0%
GCATTGCATGTGTTAATTAT+TGG + chr2.4:70167038-70167057 MS.gene00003:intron 30.0%
TCTAGTACTTCTACATCATT+TGG - chr2.4:70166833-70166852 None:intergenic 30.0%
TGGAGAGAATAAATGTTATG+TGG - chr2.4:70167192-70167211 None:intergenic 30.0%
TTTGATCTTAAATCCTGCTA+AGG + chr2.4:70166570-70166589 MS.gene00003:intron 30.0%
! ATGATTTTTGATACATAGGG+TGG + chr2.4:70166764-70166783 MS.gene00003:intron 30.0%
! CGAATCTTTTACGTTGTTAA+TGG - chr2.4:70168664-70168683 None:intergenic 30.0%
! TTTTCCTTCTTTGCAATAGT+TGG - chr2.4:70168520-70168539 None:intergenic 30.0%
!! ACTTGCTTTTCCAAAACTTA+AGG - chr2.4:70168039-70168058 None:intergenic 30.0%
!! GATTCGACAAAGTTTTATGT+TGG - chr2.4:70166644-70166663 None:intergenic 30.0%
!! TCACCCAATTCTATTTCTTT+TGG + chr2.4:70168148-70168167 MS.gene00003:intron 30.0%
!!! AAATTCGCCACTTTTTTCAT+AGG + chr2.4:70168340-70168359 MS.gene00003:CDS 30.0%
!!! ACTAGAGCAATTTTTTCCAA+TGG + chr2.4:70168456-70168475 MS.gene00003:CDS 30.0%
AAAACCAGAGAAGACTAACT+GGG + chr2.4:70168536-70168555 MS.gene00003:CDS 35.0%
AAACAAGATGGCACAAAACT+GGG + chr2.4:70168430-70168449 MS.gene00003:CDS 35.0%
AACACATGCAATGCATCAAA+CGG - chr2.4:70167032-70167051 None:intergenic 35.0%
AACATGTCTGTTCAAAGTGA+TGG + chr2.4:70168840-70168859 MS.gene00003:CDS 35.0%
ACGAATGAAGAAGAAAGAGA+TGG + chr2.4:70168582-70168601 MS.gene00003:CDS 35.0%
AGGAACAAGGAAAAGAAGAA+AGG + chr2.4:70168383-70168402 MS.gene00003:CDS 35.0%
ATATGCATGTTGGTCATAGT+AGG + chr2.4:70168790-70168809 MS.gene00003:CDS 35.0%
ATGGAATTACCATGTTGTCT+AGG - chr2.4:70168645-70168664 None:intergenic 35.0%
GGTATGAACTCATCAATAAG+TGG - chr2.4:70166899-70166918 None:intergenic 35.0%
GTCTGGTAATTAAAAGGACA+GGG + chr2.4:70166596-70166615 MS.gene00003:intron 35.0%
TACACCAACTATTGCAAAGA+AGG + chr2.4:70168513-70168532 MS.gene00003:CDS 35.0%
TACTATGACCAACATGCATA+TGG - chr2.4:70168791-70168810 None:intergenic 35.0%
TATGCATGTTGGTCATAGTA+GGG + chr2.4:70168791-70168810 MS.gene00003:CDS 35.0%
TCACTTTGAACAGACATGTT+TGG - chr2.4:70168840-70168859 None:intergenic 35.0%
TTGAAGGCCTATGAAAAAAG+TGG - chr2.4:70168350-70168369 None:intergenic 35.0%
TTGCCAATTCAAGCATTCAT+TGG - chr2.4:70167816-70167835 None:intergenic 35.0%
TTTCTTTGCTTGCAATTGCA+AGG - chr2.4:70167619-70167638 None:intergenic 35.0%
! TGCAAACAAAGTAAGAGTAC+TGG + chr2.4:70168890-70168909 MS.gene00003:CDS 35.0%
!! TGTAGAAGTACTAGAGCATT+TGG + chr2.4:70166838-70166857 MS.gene00003:CDS 35.0%
!! TGTCCTTTTAATTACCAGAC+CGG - chr2.4:70166596-70166615 None:intergenic 35.0%
!!! TTTTCCAATGGTGTTGTGAA+GGG + chr2.4:70168468-70168487 MS.gene00003:CDS 35.0%
!!! TTTTTCCAATGGTGTTGTGA+AGG + chr2.4:70168467-70168486 MS.gene00003:CDS 35.0%
AAAAACCTGGTAGTTGTGCT+GGG - chr2.4:70166958-70166977 None:intergenic 40.0%
AAATCCCTTCACAACACCAT+TGG - chr2.4:70168475-70168494 None:intergenic 40.0%
AAGCATACACAACAGGAACA+AGG + chr2.4:70168370-70168389 MS.gene00003:CDS 40.0%
AAGGTTCACACTGATGAAGA+AGG + chr2.4:70168402-70168421 MS.gene00003:CDS 40.0%
ATCTTAAATCCTGCTAAGGC+CGG + chr2.4:70166574-70166593 MS.gene00003:intron 40.0%
ATGACCACCAAACTCTCAAT+AGG + chr2.4:70167840-70167859 MS.gene00003:intron 40.0%
CTTCTTCATTCGTACCAAGA+TGG - chr2.4:70168575-70168594 None:intergenic 40.0%
CTTGCAATTGCAAGGACTAA+GGG - chr2.4:70167611-70167630 None:intergenic 40.0%
GAAAACCAGAGAAGACTAAC+TGG + chr2.4:70168535-70168554 MS.gene00003:CDS 40.0%
GAAAATTACACACTCTAGGC+TGG - chr2.4:70167746-70167765 None:intergenic 40.0%
GAAACAAGATGGCACAAAAC+TGG + chr2.4:70168429-70168448 MS.gene00003:CDS 40.0%
GCCTGAAAATTACACACTCT+AGG - chr2.4:70167750-70167769 None:intergenic 40.0%
GGTCTGGTAATTAAAAGGAC+AGG + chr2.4:70166595-70166614 MS.gene00003:intron 40.0%
GTGATGCATCAATACCATCT+TGG + chr2.4:70168558-70168577 MS.gene00003:CDS 40.0%
TAAAAACCTGGTAGTTGTGC+TGG - chr2.4:70166959-70166978 None:intergenic 40.0%
TCAATATTATGGCCGCATGA+GGG - chr2.4:70166500-70166519 None:intergenic 40.0%
TCCACAAGAGCTACATTGTT+GGG - chr2.4:70168738-70168757 None:intergenic 40.0%
TGACCACCAAACTCTCAATA+GGG + chr2.4:70167841-70167860 MS.gene00003:intron 40.0%
TGTAACACACTTCAACGAGT+TGG - chr2.4:70167788-70167807 None:intergenic 40.0%
TGTTGGGATGTTTACACTCT+TGG + chr2.4:70168077-70168096 MS.gene00003:intron 40.0%
TTCAATATTATGGCCGCATG+AGG - chr2.4:70166501-70166520 None:intergenic 40.0%
TTCCACAAGAGCTACATTGT+TGG - chr2.4:70168739-70168758 None:intergenic 40.0%
! CCTGTTGTGTATGCTTTTGA+AGG - chr2.4:70168366-70168385 None:intergenic 40.0%
! CCTTCAAAAGCATACACAAC+AGG + chr2.4:70168363-70168382 MS.gene00003:CDS 40.0%
! GCCTAGAGTGTGTAATTTTC+AGG + chr2.4:70167746-70167765 MS.gene00003:intron 40.0%
! GCTAACTCCTGCAACATTTT+AGG - chr2.4:70168310-70168329 None:intergenic 40.0%
!! AGAAGTACTAGAGCATTTGG+AGG + chr2.4:70166841-70166860 MS.gene00003:CDS 40.0%
!! TTTTATGTTGGCTGTTGTCG+AGG - chr2.4:70166632-70166651 None:intergenic 40.0%
!!! ATAGGGGCATCCTATTTTGA+CGG + chr2.4:70167858-70167877 MS.gene00003:intron 40.0%
AGAGACGATCTTGTTCTGCA+CGG + chr2.4:70167062-70167081 MS.gene00003:intron 45.0%
AGGCCGGTCTGGTAATTAAA+AGG + chr2.4:70166590-70166609 MS.gene00003:intron 45.0%
CAATATTATGGCCGCATGAG+GGG - chr2.4:70166499-70166518 None:intergenic 45.0%
CACAAGAGCTACATTGTTGG+GGG - chr2.4:70168736-70168755 None:intergenic 45.0%
CCAACGCTTCAAGGAGATAA+TGG + chr2.4:70166917-70166936 MS.gene00003:CDS 45.0%
CCACAAGAGCTACATTGTTG+GGG - chr2.4:70168737-70168756 None:intergenic 45.0%
CCATTATCTCCTTGAAGCGT+TGG - chr2.4:70166920-70166939 None:intergenic 45.0%
GAAAAAGCCCCCGTCAAAAT+AGG - chr2.4:70167871-70167890 None:intergenic 45.0%
GACCACCAAACTCTCAATAG+GGG + chr2.4:70167842-70167861 MS.gene00003:intron 45.0%
GAGTTCATACCAACGCTTCA+AGG + chr2.4:70166908-70166927 MS.gene00003:CDS 45.0%
GCTTGCAATTGCAAGGACTA+AGG - chr2.4:70167612-70167631 None:intergenic 45.0%
GGGCTGCACTGTTATTATCA+TGG - chr2.4:70168716-70168735 None:intergenic 45.0%
GGGTGGAGTTAATGAATTGC+CGG + chr2.4:70166781-70166800 MS.gene00003:intron 45.0%
GGTGGAGTTAATGAATTGCC+GGG + chr2.4:70166782-70166801 MS.gene00003:CDS 45.0%
GTCGAATCTCTACGACTCTA+TGG + chr2.4:70166656-70166675 MS.gene00003:intron 45.0%
GTGGAGTTAATGAATTGCCG+GGG + chr2.4:70166783-70166802 MS.gene00003:CDS 45.0%
GTTGCAGGAGTTAGCAAAGA+TGG + chr2.4:70168315-70168334 MS.gene00003:intron 45.0%
! AATTTGATGCATGCGGACTG+CGG - chr2.4:70167090-70167109 None:intergenic 45.0%
!! ATCACCCAGTTAGTCTTCTC+TGG - chr2.4:70168543-70168562 None:intergenic 45.0%
!!! TAGGGGCATCCTATTTTGAC+GGG + chr2.4:70167859-70167878 MS.gene00003:intron 45.0%
!!! AATAGATTTTTTTAATAAAA+TGG + chr2.4:70167485-70167504 MS.gene00003:intron 5.0%
ACCATTGAATGCCCCTCATG+CGG + chr2.4:70166485-70166504 MS.gene00003:CDS 50.0%
CAGACACAGCCTAGACAACA+TGG + chr2.4:70168633-70168652 MS.gene00003:CDS 50.0%
CATGTTGTCTAGGCTGTGTC+TGG - chr2.4:70168635-70168654 None:intergenic 50.0%
CCCCAACAATGTAGCTCTTG+TGG + chr2.4:70168734-70168753 MS.gene00003:CDS 50.0%
GGATGCCCCTATTGAGAGTT+TGG - chr2.4:70167850-70167869 None:intergenic 50.0%
GGGTCAAATTTCACTCCAGC+TGG - chr2.4:70166812-70166831 None:intergenic 50.0%
TGCCCCTATTGAGAGTTTGG+TGG - chr2.4:70167847-70167866 None:intergenic 50.0%
!!! AGGGGCATCCTATTTTGACG+GGG + chr2.4:70167860-70167879 MS.gene00003:intron 50.0%
AAATCCTGCTAAGGCCGGTC+TGG + chr2.4:70166579-70166598 MS.gene00003:intron 55.0%
ACACACCCAGCACAACTACC+AGG + chr2.4:70166950-70166969 MS.gene00003:CDS 55.0%
ATTACCAGACCGGCCTTAGC+AGG - chr2.4:70166586-70166605 None:intergenic 55.0%
TTCACTCCAGCTGGTAACCC+CGG - chr2.4:70166803-70166822 None:intergenic 55.0%
!!! GGGGCATCCTATTTTGACGG+GGG + chr2.4:70167861-70167880 MS.gene00003:intron 55.0%
GAATTGCCGGGGTTACCAGC+TGG + chr2.4:70166794-70166813 MS.gene00003:CDS 60.0%
!! GCCGCATGAGGGGCATTCAA+TGG - chr2.4:70166489-70166508 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 70166401 70168923 70166401 ID=MS.gene00003
chr2.4 mRNA 70166401 70168923 70166401 ID=MS.gene00003.t1;Parent=MS.gene00003
chr2.4 exon 70166401 70166532 70166401 ID=MS.gene00003.t1.exon1;Parent=MS.gene00003.t1
chr2.4 CDS 70166401 70166532 70166401 ID=cds.MS.gene00003.t1;Parent=MS.gene00003.t1
chr2.4 exon 70166782 70166971 70166782 ID=MS.gene00003.t1.exon2;Parent=MS.gene00003.t1
chr2.4 CDS 70166782 70166971 70166782 ID=cds.MS.gene00003.t1;Parent=MS.gene00003.t1
chr2.4 exon 70168322 70168923 70168322 ID=MS.gene00003.t1.exon3;Parent=MS.gene00003.t1
chr2.4 CDS 70168322 70168923 70168322 ID=cds.MS.gene00003.t1;Parent=MS.gene00003.t1
Gene Sequence

>MS.gene00003

ATGGCATGGTGCAATGATAAAGATAACAACAACTCTGATGTCACTAATAACATAAAGCTCAATGAAGAAATTGATCAAAATCAAACCATTGAATGCCCCTCATGCGGCCATAATATTGAAGTAAAAAATCAGGTACTAATCAATGGTTTTATTTGTTTTACTTATTTAGTTTGATCTTAAATCCTGCTAAGGCCGGTCTGGTAATTAAAAGGACAGGGTTGTGTTAGACCTCGACAACAGCCAACATAAAACTTTGTCGAATCTCTACGACTCTATGGTTAATTAAATTTGATGTTAGTTTTATTTTGTTTGTTTATGCACCAATATTAACTCCTTTAGTTTATTAATTTGTGTGTAAATTTGATGATTTTTGATACATAGGGTGGAGTTAATGAATTGCCGGGGTTACCAGCTGGAGTGAAATTTGACCCAAATGATGTAGAAGTACTAGAGCATTTGGAGGCAAAAGTGATGTCTGATGTTCGCAAGATTCATCCACTTATTGATGAGTTCATACCAACGCTTCAAGGAGATAATGGAATTTGTTATACACACCCAGCACAACTACCAGGTTTTTACATTTAATTTAATTTATTTTTTAATTAACATAATTTAGAGATTTGGAATACCGTTTGATGCATTGCATGTGTTAATTATTGGGAGAGACGATCTTGTTCTGCACGGTACCGCAGTCCGCATGCATCAAATTAATTAACTAATTAATGTTTTACAATTTAATAGTTTTTTTAATTGAAATAGATTTTAATTATAAGGCGGTTTGAGCATGACCACATAACATTTATTCTCTCCACATTTAGATCTCATAAATTTATAGAAGTAGTTCTTTACTTAGGATGCGTTTGTTTCAGCTTTAAAAAAAAATAGATTTTGTGTCAAGAGAATCTAGAGTTTTGTTTTTAGAGATTTTTAAAAAAATCTGAAATTATTTGTGTTTGCCTACTATCCATAAAAATCCATTTTTAAAAAAAATGAGTTCTAACATGCACATGTGTTATCTCCTTGTAGTTGAAAAAAATAGATTTTGAGAAAAGCTAATCCTAATAGCTTTTCATTTTAGGATCAAAATAGATTTTTTTAATAAAATGGTTTTTCTAAAAATCCTAAACAAACACTTAAAAATGAAAAAAGTGATTTTTTTTTAAGGGAAAAAATAGATTTTTTTTGAAAAAAATCTGAAACAAACGGGCCCTTAGTCCTTGCAATTGCAAGCAAAGAAAATTATTTGTCAATCGGGGTTGAGTTGAGTCTATCCCTTTTTATTAACTTATGATAATTATTTTTTGTGAATTGTGGAAAACCATAATAAAAAGAAAGTTAATGTCCAGCCTAGAGTGTGTAATTTTCAGGCATCTCTTTCATATAACCAACTCGTTGAAGTGTGTTACAAGAAACCAATGAATGCTTGAATTGGCAATGCAATGACCACCAAACTCTCAATAGGGGCATCCTATTTTGACGGGGGCTTTTTCAACATACTGTATGATATAACTAATAAGCAGATTTTTTTAGATTTAACTCCAAAACATCAATAGCAATTTGAAATTGAGCCTAAGTAATTTGTTACTGTAACATTAGGTTAATTGTTAATGAAGTTAAAGACAATTAAAAATTGTTCCTTAAGTTTTGGAAAAGCAAGTACCTTTTTTTTTTTTTTGTGTTGGGATGTTTACACTCTTGGTTTGACACAGATTGAGTCTAATAAAAACTCCTTTTGATTAATAATGTATCACCCAATTCTATTTCTTTTGGTACATTGCAAAGAACAAACAAATAGCAATTTATCAGTATATTGATATATAAATATATATGTCAAATTAGAGATGAAGGTAAAACGACACTTATTTTAAAAGAGACGGAATATGCAAGAAGTTGATATAAATTGTTACCTAAAATGTTGCAGGAGTTAGCAAAGATGGTCAAATTCGCCACTTTTTTCATAGGCCTTCAAAAGCATACACAACAGGAACAAGGAAAAGAAGAAAGGTTCACACTGATGAAGAAGGAATTGAAACAAGATGGCACAAAACTGGGAAAACTAGAGCAATTTTTTCCAATGGTGTTGTGAAGGGATTTAAGAAGATACTTGTACTCTACACCAACTATTGCAAAGAAGGAAAACCAGAGAAGACTAACTGGGTGATGCATCAATACCATCTTGGTACGAATGAAGAAGAAAGAGATGGAGAATTTGTTGCTTCAAAGATCTTCTACCAGACACAGCCTAGACAACATGGTAATTCCATTAACAACGTAAAAGATTCGTATGAAAAAATAATCGACAAAGGAAGTGGCCATGATAATAACAGTGCAGCCCCCAACAATGTAGCTCTTGTGGAATGCTACAATGCTTGTTATACAAATTATGACCATATGCATGTTGGTCATAGTAGGGAAAGCACTCCACAACTGATTATACCAAACATGTCTGTTCAAAGTGATGGAACTATTATTCGCTTATCAATGGATCATGCAAACAAAGTAAGAGTACTGGAGAGAAAGTAA
Protein sequence

>MS.gene00003.t1

MAWCNDKDNNNSDVTNNIKLNEEIDQNQTIECPSCGHNIEVKNQGGVNELPGLPAGVKFDPNDVEVLEHLEAKVMSDVRKIHPLIDEFIPTLQGDNGICYTHPAQLPGVSKDGQIRHFFHRPSKAYTTGTRKRRKVHTDEEGIETRWHKTGKTRAIFSNGVVKGFKKILVLYTNYCKEGKPEKTNWVMHQYHLGTNEEERDGEFVASKIFYQTQPRQHGNSINNVKDSYEKIIDKGSGHDNNSAAPNNVALVECYNACYTNYDHMHVGHSRESTPQLIIPNMSVQSDGTIIRLSMDHANKVRVLERK