Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000056.t1 | RHN66404.1 | 86.1 | 187 | 26 | 0 | 1 | 187 | 1 | 187 | 3.60E-72 | 281.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000056.t1 | Q8GTS1 | 46.1 | 167 | 81 | 4 | 4 | 162 | 10 | 175 | 1.3e-25 | 117.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000056.t1 | A0A396IPX9 | 86.1 | 187 | 26 | 0 | 1 | 187 | 1 | 187 | 2.6e-72 | 281.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene000056.t1 | TF | bZIP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000056.t1 | MTR_4g073100 | 45.320 | 203 | 85 | 5 | 1 | 183 | 1 | 197 | 1.29e-51 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000056.t1 | AT3G51960 | 50.000 | 168 | 74 | 4 | 4 | 162 | 10 | 176 | 2.29e-48 | 157 |
| MS.gene000056.t1 | AT3G51960 | 50.299 | 167 | 74 | 4 | 4 | 162 | 10 | 175 | 3.66e-48 | 157 |
| MS.gene000056.t1 | AT3G51960 | 56.250 | 128 | 50 | 1 | 41 | 162 | 10 | 137 | 5.04e-48 | 155 |
| MS.gene000056.t1 | AT2G16770 | 51.974 | 152 | 60 | 5 | 30 | 169 | 19 | 169 | 5.01e-46 | 152 |
| MS.gene000056.t1 | AT2G16770 | 51.974 | 152 | 60 | 5 | 30 | 169 | 19 | 169 | 5.01e-46 | 152 |
| MS.gene000056.t1 | AT4G35040 | 46.703 | 182 | 75 | 6 | 1 | 165 | 1 | 177 | 3.62e-45 | 150 |
| MS.gene000056.t1 | AT4G35040 | 46.703 | 182 | 75 | 6 | 1 | 165 | 1 | 177 | 3.62e-45 | 150 |
| MS.gene000056.t1 | AT4G35040 | 46.703 | 182 | 75 | 6 | 1 | 165 | 1 | 177 | 3.62e-45 | 150 |
| MS.gene000056.t1 | AT4G35040 | 46.703 | 182 | 75 | 6 | 1 | 165 | 1 | 177 | 3.62e-45 | 150 |
Find 22 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGGCAGAAACAGCTTATT+TGG | 0.185081 | 3.1:+25004633 | MS.gene000056:CDS |
| TGAAAGTTGCTACATGATTC+TGG | 0.229394 | 3.1:-25004388 | None:intergenic |
| GGAAAAGTTGACAATGAATT+AGG | 0.247864 | 3.1:+25004772 | MS.gene000056:CDS |
| GGCAAGGTTAAGGAACATTT+TGG | 0.264303 | 3.1:+25004738 | MS.gene000056:CDS |
| AAAGTATAGGGAGAAGAAAA+AGG | 0.319200 | 3.1:+25004615 | MS.gene000056:CDS |
| GTAGTTTCCTTAAAAGTTGT+TGG | 0.338670 | 3.1:-25004687 | None:intergenic |
| GGCACTGCTTGAAGCTGAAT+TGG | 0.353982 | 3.1:+25004717 | MS.gene000056:CDS |
| AAGCTGAATTGGCAAGGTTA+AGG | 0.385542 | 3.1:+25004728 | MS.gene000056:CDS |
| ACACAAATTCAAGGCCAAAA+AGG | 0.410549 | 3.1:+25004566 | MS.gene000056:CDS |
| ACATGCAGGATCATCATCTA+AGG | 0.417339 | 3.1:+25004834 | MS.gene000056:CDS |
| AAGAAAGAAAATATGGAAGA+TGG | 0.479572 | 3.1:+25004316 | None:intergenic |
| CTGAAGATGACACAAATTCA+AGG | 0.502147 | 3.1:+25004557 | MS.gene000056:CDS |
| GTCCTTTAGTACTACTCATG+TGG | 0.520570 | 3.1:-25004880 | None:intergenic |
| CCATGTTTGCATCCACATGC+AGG | 0.525489 | 3.1:+25004820 | MS.gene000056:CDS |
| TTTAAGGAAACTACAAGGGC+AGG | 0.534031 | 3.1:+25004696 | MS.gene000056:CDS |
| CCTGCATGTGGATGCAAACA+TGG | 0.553344 | 3.1:-25004820 | None:intergenic |
| TGTGTGTGATAAGCATCATC+AGG | 0.565504 | 3.1:-25004499 | None:intergenic |
| TGCTTGAAGCTGAATTGGCA+AGG | 0.581638 | 3.1:+25004722 | MS.gene000056:CDS |
| TGATAAGCATCATCAGGACC+AGG | 0.614933 | 3.1:-25004493 | None:intergenic |
| TTAGATGATGATCCTGCATG+TGG | 0.661059 | 3.1:-25004832 | None:intergenic |
| ACTCACACTTGTAATCCACC+TGG | 0.661329 | 3.1:+25004475 | MS.gene000056:CDS |
| TAAGCATCATCAGGACCAGG+TGG | 0.696090 | 3.1:-25004490 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAGCTGTTAAAAAGTATA+GGG | + | chr3.1:25004603-25004622 | MS.gene000056:CDS | 25.0% |
| !!! | AACTTTTAAGGAAACTACAA+GGG | + | chr3.1:25004692-25004711 | MS.gene000056:CDS | 25.0% |
| AAAGTATAGGGAGAAGAAAA+AGG | + | chr3.1:25004615-25004634 | MS.gene000056:CDS | 30.0% | |
| GGAAAAGTTGACAATGAATT+AGG | + | chr3.1:25004772-25004791 | MS.gene000056:CDS | 30.0% | |
| GTAGTTTCCTTAAAAGTTGT+TGG | - | chr3.1:25004690-25004709 | None:intergenic | 30.0% | |
| ! | ATGGCAGATTTTTCTCAAAA+GGG | - | chr3.1:25004804-25004823 | None:intergenic | 30.0% |
| ! | ATGTGAACCAACAACTTTTA+AGG | + | chr3.1:25004680-25004699 | MS.gene000056:CDS | 30.0% |
| ! | GAGAAGCTGTTAAAAAGTAT+AGG | + | chr3.1:25004602-25004621 | MS.gene000056:CDS | 30.0% |
| !! | CAACTTTTAAGGAAACTACA+AGG | + | chr3.1:25004691-25004710 | MS.gene000056:CDS | 30.0% |
| AAAGGCAGAAACAGCTTATT+TGG | + | chr3.1:25004633-25004652 | MS.gene000056:CDS | 35.0% | |
| ACACAAATTCAAGGCCAAAA+AGG | + | chr3.1:25004566-25004585 | MS.gene000056:CDS | 35.0% | |
| CTGAAGATGACACAAATTCA+AGG | + | chr3.1:25004557-25004576 | MS.gene000056:CDS | 35.0% | |
| TGAAAGTTGCTACATGATTC+TGG | - | chr3.1:25004391-25004410 | None:intergenic | 35.0% | |
| ! | CATGGCAGATTTTTCTCAAA+AGG | - | chr3.1:25004805-25004824 | None:intergenic | 35.0% |
| !! | AACATTTTGGTGCAGCTTAA+GGG | + | chr3.1:25004751-25004770 | MS.gene000056:CDS | 35.0% |
| !! | AGTGTGAATGCATGTTTTTG+TGG | - | chr3.1:25004458-25004477 | None:intergenic | 35.0% |
| AAGCTGAATTGGCAAGGTTA+AGG | + | chr3.1:25004728-25004747 | MS.gene000056:CDS | 40.0% | |
| ACATGCAGGATCATCATCTA+AGG | + | chr3.1:25004834-25004853 | MS.gene000056:CDS | 40.0% | |
| TGTGTGTGATAAGCATCATC+AGG | - | chr3.1:25004502-25004521 | None:intergenic | 40.0% | |
| TTAGATGATGATCCTGCATG+TGG | - | chr3.1:25004835-25004854 | None:intergenic | 40.0% | |
| TTTAAGGAAACTACAAGGGC+AGG | + | chr3.1:25004696-25004715 | MS.gene000056:CDS | 40.0% | |
| ! | CTATTGCCAGAAGTCCTTTT+TGG | - | chr3.1:25004583-25004602 | None:intergenic | 40.0% |
| ! | GGCAAGGTTAAGGAACATTT+TGG | + | chr3.1:25004738-25004757 | MS.gene000056:CDS | 40.0% |
| !! | GAACATTTTGGTGCAGCTTA+AGG | + | chr3.1:25004750-25004769 | MS.gene000056:CDS | 40.0% |
| ACTCACACTTGTAATCCACC+TGG | + | chr3.1:25004475-25004494 | MS.gene000056:CDS | 45.0% | |
| TCAAGGCCAAAAAGGACTTC+TGG | + | chr3.1:25004574-25004593 | MS.gene000056:CDS | 45.0% | |
| TGATAAGCATCATCAGGACC+AGG | - | chr3.1:25004496-25004515 | None:intergenic | 45.0% | |
| TGCTTGAAGCTGAATTGGCA+AGG | + | chr3.1:25004722-25004741 | MS.gene000056:CDS | 45.0% | |
| CCATGTTTGCATCCACATGC+AGG | + | chr3.1:25004820-25004839 | MS.gene000056:CDS | 50.0% | |
| CCTGCATGTGGATGCAAACA+TGG | - | chr3.1:25004823-25004842 | None:intergenic | 50.0% | |
| GGCACTGCTTGAAGCTGAAT+TGG | + | chr3.1:25004717-25004736 | MS.gene000056:CDS | 50.0% | |
| TAAGCATCATCAGGACCAGG+TGG | - | chr3.1:25004493-25004512 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 25004328 | 25004891 | 25004328 | ID=MS.gene000056 |
| chr3.1 | mRNA | 25004328 | 25004891 | 25004328 | ID=MS.gene000056.t1;Parent=MS.gene000056 |
| chr3.1 | exon | 25004328 | 25004891 | 25004328 | ID=MS.gene000056.t1.exon1;Parent=MS.gene000056.t1 |
| chr3.1 | CDS | 25004328 | 25004891 | 25004328 | ID=cds.MS.gene000056.t1;Parent=MS.gene000056.t1 |
| Gene Sequence |
| Protein sequence |
