Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000085.t1 | RHN59513.1 | 91.1 | 45 | 4 | 0 | 4 | 48 | 31 | 75 | 7.10E-12 | 79.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000085.t1 | Q9FJK3 | 53.9 | 76 | 31 | 1 | 4 | 79 | 3 | 74 | 6.1e-12 | 71.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000085.t1 | G7JN52 | 91.1 | 45 | 4 | 0 | 4 | 48 | 3 | 47 | 5.1e-12 | 79.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene000085.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000085.t1 | MTR_8g036130 | 80.000 | 45 | 9 | 0 | 4 | 48 | 3 | 47 | 1.32e-17 | 74.3 |
| MS.gene000085.t1 | MTR_1g007990 | 75.556 | 45 | 11 | 0 | 41 | 85 | 912 | 956 | 2.52e-17 | 75.1 |
| MS.gene000085.t1 | MTR_4g032620 | 75.000 | 48 | 11 | 1 | 1 | 48 | 1 | 47 | 3.74e-17 | 72.8 |
| MS.gene000085.t1 | MTR_1g090783 | 54.839 | 62 | 24 | 1 | 4 | 65 | 2 | 59 | 5.13e-16 | 68.6 |
| MS.gene000085.t1 | MTR_1g090697 | 50.704 | 71 | 31 | 1 | 4 | 74 | 3 | 69 | 1.55e-15 | 67.0 |
| MS.gene000085.t1 | MTR_5g047580 | 54.839 | 62 | 24 | 1 | 4 | 65 | 3 | 60 | 3.15e-15 | 66.2 |
| MS.gene000085.t1 | MTR_3g466830 | 68.889 | 45 | 14 | 0 | 4 | 48 | 2 | 46 | 1.45e-14 | 64.7 |
| MS.gene000085.t1 | MTR_2g016210 | 72.093 | 43 | 12 | 0 | 6 | 48 | 5 | 47 | 1.49e-14 | 66.2 |
| MS.gene000085.t1 | MTR_4g028800 | 74.419 | 43 | 9 | 1 | 6 | 48 | 5 | 45 | 1.70e-14 | 62.4 |
| MS.gene000085.t1 | MTR_1g077390 | 66.667 | 48 | 15 | 1 | 4 | 50 | 3 | 50 | 3.16e-14 | 63.9 |
| MS.gene000085.t1 | MTR_3g466890 | 68.889 | 45 | 14 | 0 | 4 | 48 | 2 | 46 | 6.97e-14 | 63.2 |
| MS.gene000085.t1 | MTR_3g466980 | 64.444 | 45 | 16 | 0 | 4 | 48 | 3 | 47 | 7.71e-14 | 63.9 |
| MS.gene000085.t1 | MTR_3g031240 | 40.506 | 79 | 42 | 2 | 1 | 79 | 1 | 74 | 1.13e-12 | 60.5 |
| MS.gene000085.t1 | MTR_4g031910 | 82.222 | 45 | 8 | 0 | 4 | 48 | 3 | 47 | 2.30e-12 | 60.1 |
| MS.gene000085.t1 | MTR_5g075380 | 62.791 | 43 | 16 | 0 | 6 | 48 | 5 | 47 | 2.37e-12 | 58.9 |
| MS.gene000085.t1 | MTR_4g063790 | 54.348 | 46 | 21 | 0 | 4 | 49 | 3 | 48 | 2.56e-12 | 59.3 |
| MS.gene000085.t1 | MTR_1g090710 | 48.438 | 64 | 28 | 2 | 1 | 64 | 1 | 59 | 4.68e-12 | 58.2 |
| MS.gene000085.t1 | MTR_2g035610 | 78.261 | 46 | 10 | 0 | 4 | 49 | 3 | 48 | 5.61e-12 | 57.4 |
| MS.gene000085.t1 | MTR_3g065100 | 62.295 | 61 | 19 | 1 | 4 | 64 | 3 | 59 | 2.63e-11 | 57.4 |
| MS.gene000085.t1 | MTR_7g011950 | 47.561 | 82 | 39 | 1 | 4 | 85 | 3 | 80 | 5.18e-11 | 55.5 |
| MS.gene000085.t1 | MTR_4g019670 | 39.024 | 82 | 46 | 1 | 3 | 84 | 5 | 82 | 5.64e-11 | 57.0 |
| MS.gene000085.t1 | MTR_4g028720 | 48.101 | 79 | 37 | 1 | 4 | 82 | 3 | 77 | 7.92e-11 | 55.1 |
| MS.gene000085.t1 | MTR_3g031100 | 43.939 | 66 | 32 | 2 | 1 | 66 | 1 | 61 | 8.45e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000085.t1 | AT3G05860 | 43.902 | 82 | 41 | 2 | 4 | 85 | 3 | 79 | 1.31e-14 | 65.9 |
| MS.gene000085.t1 | AT3G05860 | 43.902 | 82 | 41 | 2 | 4 | 85 | 3 | 79 | 1.87e-14 | 65.9 |
| MS.gene000085.t1 | AT3G05860 | 43.902 | 82 | 41 | 2 | 4 | 85 | 3 | 79 | 2.10e-14 | 65.9 |
| MS.gene000085.t1 | AT1G22590 | 42.308 | 78 | 37 | 3 | 1 | 75 | 1 | 73 | 2.78e-12 | 58.9 |
| MS.gene000085.t1 | AT1G65330 | 55.556 | 45 | 20 | 0 | 4 | 48 | 2 | 46 | 4.85e-11 | 57.0 |
| MS.gene000085.t1 | AT5G26580 | 41.096 | 73 | 41 | 2 | 1 | 72 | 1 | 72 | 5.37e-11 | 57.0 |
| MS.gene000085.t1 | AT5G26650 | 52.941 | 51 | 23 | 1 | 6 | 55 | 3 | 53 | 7.58e-11 | 56.6 |
| MS.gene000085.t1 | AT5G27960 | 52.941 | 51 | 23 | 1 | 6 | 55 | 3 | 53 | 8.28e-11 | 56.6 |
| MS.gene000085.t1 | AT5G48670 | 53.947 | 76 | 31 | 1 | 4 | 79 | 3 | 74 | 8.30e-11 | 56.6 |
Find 0 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCATATTTGTATTATGTAT+AGG | + | 33310:2930-2949 | MS.gene000085:intron | 15.0% |
| !! | TAAAAAACAGAATAGTTAGA+TGG | - | 33310:2973-2992 | MS.gene000085:intergenic | 20.0% |
| !!! | ATCTAACTATTCTGTTTTTT+AGG | + | 33310:2972-2991 | MS.gene000085:intron | 20.0% |
| !! | GTATTATGTATAGGTTTTTG+AGG | + | 33310:2939-2958 | MS.gene000085:intron | 25.0% |
| GAAGAAGAGTCTATTCAAAA+AGG | + | 33310:2815-2834 | MS.gene000085:CDS | 30.0% | |
| ! | CTTGCATATATTTTCCTTGT+GGG | + | 33310:2999-3018 | MS.gene000085:CDS | 30.0% |
| CCACAAGGAAAATATATGCA+AGG | - | 33310:3001-3020 | MS.gene000084:intergenic | 35.0% | |
| GGAAAGCAACATACAAGAAA+AGG | + | 33310:2793-2812 | MS.gene000085:CDS | 35.0% | |
| TCATTGTCAATGATGCTTCT+AGG | + | 33310:2772-2791 | MS.gene000085:CDS | 35.0% | |
| ! | CCTTGCATATATTTTCCTTG+TGG | + | 33310:2998-3017 | MS.gene000085:CDS | 35.0% |
| !! | AGAAGCATCATTGACAATGA+AGG | - | 33310:2772-2791 | MS.gene000085:intergenic | 35.0% |
| ACAAGCCTCAATACCACATA+GGG | - | 33310:2856-2875 | MS.gene000084:intergenic | 40.0% | |
| ATATCCATCTTGTTCCCACA+AGG | - | 33310:3016-3035 | MS.gene000084:intergenic | 40.0% | |
| GAAATGCAGAACTCTCGTAA+AGG | - | 33310:2893-2912 | MS.gene000084:intergenic | 40.0% | |
| TTCAACATTTCCGTAGCTTC+TGG | - | 33310:3043-3062 | MS.gene000085:intergenic | 40.0% | |
| ! | TTTTCCTTGTGGGAACAAGA+TGG | + | 33310:3009-3028 | MS.gene000085:CDS | 40.0% |
| AACATTTCCGTAGCTTCTGG+AGG | - | 33310:3040-3059 | MS.gene000084:intergenic | 45.0% | |
| CACAAGCCTCAATACCACAT+AGG | - | 33310:2857-2876 | MS.gene000084:intergenic | 45.0% | |
| CTCGTAAAGGCCATACACTA+TGG | - | 33310:2880-2899 | MS.gene000084:intergenic | 45.0% | |
| GGATATTCCTCCAGAAGCTA+CGG | + | 33310:3030-3049 | MS.gene000085:CDS | 45.0% | |
| GTTGAACTTAGCACCCTATG+TGG | + | 33310:2840-2859 | MS.gene000085:CDS | 45.0% | |
| TAGCACCCTATGTGGTATTG+AGG | + | 33310:2848-2867 | MS.gene000085:CDS | 45.0% | |
| GAGGCTTGTGCCATAGTGTA+TGG | + | 33310:2867-2886 | MS.gene000085:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 33310 | gene | 2741 | 3106 | 2741 | ID=MS.gene000085 |
| 33310 | mRNA | 2741 | 3106 | 2741 | ID=MS.gene000085.t1;Parent=MS.gene000085 |
| 33310 | exon | 2741 | 2883 | 2741 | ID=MS.gene000085.t1.exon1;Parent=MS.gene000085.t1 |
| 33310 | CDS | 2741 | 2883 | 2741 | ID=cds.MS.gene000085.t1;Parent=MS.gene000085.t1 |
| 33310 | exon | 2994 | 3106 | 2994 | ID=MS.gene000085.t1.exon2;Parent=MS.gene000085.t1 |
| 33310 | CDS | 2994 | 3106 | 2994 | ID=cds.MS.gene000085.t1;Parent=MS.gene000085.t1 |
| Gene Sequence |
| Protein sequence |
