AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000185

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000185.t1 MTR_2g062310 93.692 428 26 1 1 428 1 427 0.0 793
MS.gene000185.t1 MTR_2g084275 45.055 91 49 1 9 99 31 120 5.99e-21 95.5
MS.gene000185.t1 MTR_4g063130 42.857 91 51 1 9 99 33 122 1.51e-20 93.6
MS.gene000185.t1 MTR_4g063130 42.857 91 51 1 9 99 33 122 2.09e-20 93.2
MS.gene000185.t1 MTR_4g057330 45.556 90 48 1 10 99 31 119 3.00e-20 93.2
MS.gene000185.t1 MTR_1g115370 42.105 95 53 2 10 103 13 106 1.48e-17 82.4
MS.gene000185.t1 MTR_8g091300 41.000 100 57 2 1 99 22 120 4.11e-17 83.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000185.t1 AT1G05577 35.897 429 178 15 5 417 7 354 2.70e-63 208
MS.gene000185.t1 AT2G28150 46.739 92 48 1 8 99 31 121 1.60e-23 103
MS.gene000185.t1 AT5G10150 39.474 114 67 2 1 114 36 147 3.10e-21 95.5
MS.gene000185.t1 AT3G46110 43.878 98 54 1 2 99 11 107 7.02e-21 93.2
MS.gene000185.t1 AT3G46110 43.878 98 54 1 2 99 11 107 7.29e-21 93.6
MS.gene000185.t1 AT5G10150 39.823 113 66 2 1 113 36 146 8.06e-21 94.4
MS.gene000185.t1 AT5G59790 41.758 91 52 1 9 99 45 134 1.17e-20 94.0
MS.gene000185.t1 AT5G59790 41.758 91 52 1 9 99 45 134 1.40e-20 93.2

Find 68 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGATCAAAATGAACATTA+TGG 0.310101 2.1:+33651328 MS.gene000185:CDS
CAAATCCACACTACCCCTTT+TGG 0.310924 2.1:+33650440 MS.gene000185:CDS
ATATGTGTGGCAGGATTTAA+TGG 0.312486 2.1:+33650364 MS.gene000185:CDS
GGAGGATCAGCTAGGTGCTT+TGG 0.328431 2.1:+33651289 MS.gene000185:CDS
TACGACCAAGCAAAGGATTC+AGG 0.352565 2.1:-33650206 None:intergenic
GAGGATCAGCTAGGTGCTTT+GGG 0.353768 2.1:+33651290 MS.gene000185:CDS
GGCACATTAATAGGCTCCTT+AGG 0.355225 2.1:-33651229 None:intergenic
CTAGGTGCTTTGGGACTACT+TGG 0.358486 2.1:+33651299 MS.gene000185:CDS
GACGTATTCATTATCAGAAA+TGG 0.358743 2.1:-33650405 None:intergenic
CAAACTTCTTATAAACCATT+TGG 0.361784 2.1:+33651793 MS.gene000185:CDS
AGAAATCAGTCAGGATTCAT+TGG 0.387998 2.1:+33650811 MS.gene000185:CDS
ACGTATTCATTATCAGAAAT+GGG 0.408507 2.1:-33650404 None:intergenic
TCAGTTACTTACGAGCAAAT+TGG 0.422889 2.1:-33651820 None:intergenic
CAAAAGGTGGAGAAGTTAGA+AGG 0.438816 2.1:+33649514 MS.gene000185:CDS
GCACATTAATAGGCTCCTTA+GGG 0.446658 2.1:-33651228 None:intergenic
GCTTCTAATCTTAGTGAATG+AGG 0.454114 2.1:-33651101 None:intergenic
TGTGGATTTGTGATCCTTTG+AGG 0.457496 2.1:-33650427 None:intergenic
ACTTCTTATAAACCATTTGG+TGG 0.460136 2.1:+33651796 MS.gene000185:CDS
AAAGATATTAAGAGATGGCT+TGG 0.473793 2.1:+33650161 MS.gene000185:intron
GATTCTTCTTCTGATAAAGA+TGG 0.475364 2.1:-33650734 None:intergenic
GTTCTTCATCTTGCTCGAAA+CGG 0.488480 2.1:+33649600 MS.gene000185:CDS
TGCTCAGAAAATTGCCCCTA+AGG 0.491836 2.1:+33651213 MS.gene000185:CDS
CATAATTTGATGAGTAAGAA+GGG 0.494107 2.1:+33650980 MS.gene000185:CDS
TGATAAAGTACATACCAAAA+GGG 0.495189 2.1:-33650454 None:intergenic
GAGCAAATTGGTCCACCAAA+TGG 0.501260 2.1:-33651808 None:intergenic
TCATAATTTGATGAGTAAGA+AGG 0.513194 2.1:+33650979 MS.gene000185:CDS
CAAGAGTGGATATGTGTGGC+AGG 0.519703 2.1:+33650355 MS.gene000185:CDS
AAATCAAGCATGGAAACAAA+AGG 0.522656 2.1:+33649498 None:intergenic
AGATGGCTTGGTGAAGTGAG+AGG 0.523481 2.1:+33650173 MS.gene000185:CDS
ACCTACCTGAATCCTTTGCT+TGG 0.534515 2.1:+33650201 MS.gene000185:CDS
AAAAGGGGTGAGATTTGTAA+AGG 0.535313 2.1:+33651256 MS.gene000185:CDS
TTTCAGCTCGGACAGGTCAT+CGG 0.536641 2.1:+33650835 MS.gene000185:CDS
GAGATGAAATAATCAAGAGC+AGG 0.544676 2.1:+33651746 MS.gene000185:CDS
AAAATGAACATTATGGAGCC+AGG 0.547597 2.1:+33651335 MS.gene000185:CDS
CTTGAAAAGATATTAAGAGA+TGG 0.548408 2.1:+33650156 MS.gene000185:intron
ATCAAATTATGATACAAAGA+AGG 0.549252 2.1:-33650968 None:intergenic
CCTTTGCTTGGTCGTACAAG+AGG 0.558378 2.1:+33650213 MS.gene000185:CDS
AAACACATGAAGTCTTGTAA+AGG 0.559207 2.1:+33652597 MS.gene000185:CDS
AAGTCTTGTAAAGGAATGAA+AGG 0.559264 2.1:+33652606 MS.gene000185:CDS
TGTAAAGGAATGAAAGGCAT+GGG 0.564737 2.1:+33652612 MS.gene000185:CDS
AGTGTTATCAGAAATCAGTC+AGG 0.567696 2.1:+33650802 MS.gene000185:CDS
GATAATGAATACGTCCTCAA+AGG 0.574396 2.1:+33650413 MS.gene000185:CDS
ACAAGCTAGGAGGATCAGCT+AGG 0.576145 2.1:+33651281 MS.gene000185:CDS
ATTTGTAAAGGAGACAAGCT+AGG 0.576650 2.1:+33651268 MS.gene000185:CDS
CGTATTCATTATCAGAAATG+GGG 0.578739 2.1:-33650403 None:intergenic
ACATACCAAAAGGGGTAGTG+TGG 0.583512 2.1:-33650445 None:intergenic
TTGTAAAGGAATGAAAGGCA+TGG 0.590138 2.1:+33652611 MS.gene000185:CDS
TGAAGCTGTACAAGTTGAAG+AGG 0.593606 2.1:+33650688 MS.gene000185:CDS
GTTGATGATGCAGGCAATGT+GGG 0.595370 2.1:+33652547 MS.gene000185:intron
AATCTACTTTCTTAGTCACA+TGG 0.595550 2.1:+33649545 MS.gene000185:CDS
ATCTACTTTCTTAGTCACAT+GGG 0.598289 2.1:+33649546 MS.gene000185:CDS
GATAAAGTACATACCAAAAG+GGG 0.611244 2.1:-33650453 None:intergenic
AAGCTGTGTTTCCTCAGCAA+TGG 0.615582 2.1:-33652649 None:intergenic
TACTTTCTTAGTCACATGGG+TGG 0.621822 2.1:+33649549 MS.gene000185:CDS
TCAAGCATGGAAACAAAAGG+TGG 0.631710 2.1:+33649501 None:intergenic
TGAAGAACACGAATGAGATG+AGG 0.633710 2.1:-33649585 None:intergenic
AACTATAGGAGGTACAAGAG+TGG 0.640091 2.1:+33650341 MS.gene000185:intron
GTATTCATTATCAGAAATGG+GGG 0.640428 2.1:-33650402 None:intergenic
CACATTAATAGGCTCCTTAG+GGG 0.642016 2.1:-33651227 None:intergenic
TGAGACGATGTTGATGACGT+TGG 0.649599 2.1:-33651070 None:intergenic
CGAAACGGTGTTTATCTCAG+AGG 0.652327 2.1:+33649615 MS.gene000185:CDS
TTCAGCTACAACCATTGCTG+AGG 0.657539 2.1:+33652638 MS.gene000185:CDS
TTGGATTGGTTGTGGAACTG+TGG 0.657832 2.1:+33651156 MS.gene000185:CDS
CCTCTTGTACGACCAAGCAA+AGG 0.668791 2.1:-33650213 None:intergenic
ACCAAGCAAAGGATTCAGGT+AGG 0.684420 2.1:-33650202 None:intergenic
GGTACAAGAGTGGATATGTG+TGG 0.687025 2.1:+33650351 MS.gene000185:CDS
TGTAAAGGAGACAAGCTAGG+AGG 0.688268 2.1:+33651271 MS.gene000185:CDS
AGTTGATGATGCAGGCAATG+TGG 0.692418 2.1:+33652546 MS.gene000185:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATGTGAATAAATAAAAAT+GGG - chr2.1:33650493-33650512 None:intergenic 10.0%
!! TTAATGTGAATAAATAAAAA+TGG - chr2.1:33650494-33650513 None:intergenic 10.0%
!! AAGAAATACAAAATATAAGT+TGG + chr2.1:33652323-33652342 MS.gene000185:intron 15.0%
!! TTAGCAAAAATTACATAATA+AGG - chr2.1:33650563-33650582 None:intergenic 15.0%
!! TTAGTTACAAAAATTGATTA+TGG - chr2.1:33650312-33650331 None:intergenic 15.0%
!! AAATATACTGAAACTACAAT+TGG + chr2.1:33652207-33652226 MS.gene000185:intron 20.0%
!! ACTAACAATTATTGAACTAT+AGG + chr2.1:33650327-33650346 MS.gene000185:intron 20.0%
!! AGTTAAAGGTTAAAAAAATG+AGG - chr2.1:33650277-33650296 None:intergenic 20.0%
!! ATCAAATTATGATACAAAGA+AGG - chr2.1:33650971-33650990 None:intergenic 20.0%
!! GAACAAATTGAATGAAAATA+TGG - chr2.1:33649658-33649677 None:intergenic 20.0%
!! GTTAAAAACCATTCTTAAAA+GGG + chr2.1:33651585-33651604 MS.gene000185:intron 20.0%
!! TGTTAAAAACCATTCTTAAA+AGG + chr2.1:33651584-33651603 MS.gene000185:intron 20.0%
!!! ATGAAAAATTTTGGTCTTAT+AGG + chr2.1:33651908-33651927 MS.gene000185:intron 20.0%
!!! CCATTATTAAGTTGAAAAAA+TGG + chr2.1:33652024-33652043 MS.gene000185:intron 20.0%
!!! CCATTTTTTCAACTTAATAA+TGG - chr2.1:33652027-33652046 None:intergenic 20.0%
!!! TGATTTAGATCATTCTTTTT+TGG - chr2.1:33650782-33650801 None:intergenic 20.0%
!!! TTTTAAGAATGGTTTTTAAC+AGG - chr2.1:33651585-33651604 None:intergenic 20.0%
! AAAAAATGAGGTTGAGAAAA+TGG - chr2.1:33650265-33650284 None:intergenic 25.0%
! AACAATTATTGAACTATAGG+AGG + chr2.1:33650330-33650349 MS.gene000185:intron 25.0%
! ACGTATTCATTATCAGAAAT+GGG - chr2.1:33650407-33650426 None:intergenic 25.0%
! ATGATAAAGTACATACCAAA+AGG - chr2.1:33650458-33650477 None:intergenic 25.0%
! ATTCAAAACCATGTCTAAAA+GGG - chr2.1:33651562-33651581 None:intergenic 25.0%
! CAAACTTCTTATAAACCATT+TGG + chr2.1:33651793-33651812 MS.gene000185:CDS 25.0%
! CATAATTTGATGAGTAAGAA+GGG + chr2.1:33650980-33650999 MS.gene000185:CDS 25.0%
! CATGATCAAAATGAACATTA+TGG + chr2.1:33651328-33651347 MS.gene000185:CDS 25.0%
! CTTGAAAAGATATTAAGAGA+TGG + chr2.1:33650156-33650175 MS.gene000185:intron 25.0%
! GGAGATACATATATAGTTAA+AGG - chr2.1:33650291-33650310 None:intergenic 25.0%
! TAATGTGTCTTTACACATAT+GGG - chr2.1:33652478-33652497 None:intergenic 25.0%
! TAGAGTAAACTTCAATTGTT+TGG + chr2.1:33651852-33651871 MS.gene000185:intron 25.0%
! TATTAATGTGCCAGAAAAAA+GGG + chr2.1:33651240-33651259 MS.gene000185:CDS 25.0%
! TCATAATTTGATGAGTAAGA+AGG + chr2.1:33650979-33650998 MS.gene000185:CDS 25.0%
! TGATAAAGTACATACCAAAA+GGG - chr2.1:33650457-33650476 None:intergenic 25.0%
! TGTAGATAGATAGAAATTAG+TGG - chr2.1:33650534-33650553 None:intergenic 25.0%
! TTAATGTGTCTTTACACATA+TGG - chr2.1:33652479-33652498 None:intergenic 25.0%
!! AAACTGTGCATGAAAAATTT+TGG + chr2.1:33651899-33651918 MS.gene000185:intron 25.0%
!!! AAAAATTTTGGTCTTATAGG+TGG + chr2.1:33651911-33651930 MS.gene000185:intron 25.0%
!!! TCACATTAATGTTTTGAGTA+TGG + chr2.1:33650505-33650524 MS.gene000185:intron 25.0%
!!! TTAAGTTGAAAAAATGGAAG+TGG + chr2.1:33652030-33652049 MS.gene000185:intron 25.0%
AAAGATATTAAGAGATGGCT+TGG + chr2.1:33650161-33650180 MS.gene000185:intron 30.0%
AATCCAATGCTACTATGATT+GGG + chr2.1:33651616-33651635 MS.gene000185:intron 30.0%
AATCTACTTTCTTAGTCACA+TGG + chr2.1:33649545-33649564 MS.gene000185:CDS 30.0%
AATTGCAGATCATAGACAAT+AGG - chr2.1:33650043-33650062 None:intergenic 30.0%
ACTTCTTATAAACCATTTGG+TGG + chr2.1:33651796-33651815 MS.gene000185:CDS 30.0%
AGTAAGAAGGGTAAAAAAGA+TGG + chr2.1:33650992-33651011 MS.gene000185:CDS 30.0%
AGTGTGTTCAAAAATTGGAT+TGG + chr2.1:33651142-33651161 MS.gene000185:CDS 30.0%
ATCTACTTTCTTAGTCACAT+GGG + chr2.1:33649546-33649565 MS.gene000185:CDS 30.0%
ATTAATGTGCCAGAAAAAAG+GGG + chr2.1:33651241-33651260 MS.gene000185:CDS 30.0%
CGTATTCATTATCAGAAATG+GGG - chr2.1:33650406-33650425 None:intergenic 30.0%
CTATTAATGTGCCAGAAAAA+AGG + chr2.1:33651239-33651258 MS.gene000185:CDS 30.0%
GACGTATTCATTATCAGAAA+TGG - chr2.1:33650408-33650427 None:intergenic 30.0%
GATAAAGTACATACCAAAAG+GGG - chr2.1:33650456-33650475 None:intergenic 30.0%
GATTCAAAACCATGTCTAAA+AGG - chr2.1:33651563-33651582 None:intergenic 30.0%
GATTCTTCTTCTGATAAAGA+TGG - chr2.1:33650737-33650756 None:intergenic 30.0%
GATTTCAACCATGAAATGTT+AGG + chr2.1:33651874-33651893 MS.gene000185:intron 30.0%
GCATCATCAACTACTATTTA+TGG - chr2.1:33652538-33652557 None:intergenic 30.0%
GTATTCATTATCAGAAATGG+GGG - chr2.1:33650405-33650424 None:intergenic 30.0%
GTGTACTAAATTGTGTATCA+CGG - chr2.1:33649977-33649996 None:intergenic 30.0%
TCACACATAGTTCAAAATTG+CGG + chr2.1:33649738-33649757 MS.gene000185:intron 30.0%
TGACTAGTGTGTTCAAAAAT+TGG + chr2.1:33651137-33651156 MS.gene000185:CDS 30.0%
TTATGATACAATTTCTCACC+TGG - chr2.1:33651356-33651375 None:intergenic 30.0%
TTATGATGTTTACCAACTCT+TGG + chr2.1:33652360-33652379 MS.gene000185:intron 30.0%
! AAACACATGAAGTCTTGTAA+AGG + chr2.1:33652597-33652616 MS.gene000185:CDS 30.0%
! AAGTCTTGTAAAGGAATGAA+AGG + chr2.1:33652606-33652625 MS.gene000185:CDS 30.0%
! TCATTCAATTTGTTCTGTAC+AGG + chr2.1:33649663-33649682 MS.gene000185:intron 30.0%
! TTGAAGAAGAGAAGCTTTTA+GGG + chr2.1:33650885-33650904 MS.gene000185:CDS 30.0%
! TTTTACAGAAAAAGCTGTGA+TGG + chr2.1:33651724-33651743 MS.gene000185:intron 30.0%
!! CCTCTAAAACCTTTATATTG+CGG - chr2.1:33649771-33649790 None:intergenic 30.0%
!! TAAATAGTAGTTGATGATGC+AGG + chr2.1:33652538-33652557 MS.gene000185:intron 30.0%
!! TTCAAAAATTGGATTGGTTG+TGG + chr2.1:33651148-33651167 MS.gene000185:CDS 30.0%
!!! CTTTTTTCTGGCACATTAAT+AGG - chr2.1:33651241-33651260 None:intergenic 30.0%
!!! TCTAAGCATGGTTTTAAGTT+GGG - chr2.1:33651523-33651542 None:intergenic 30.0%
!!! TTCTAAGCATGGTTTTAAGT+TGG - chr2.1:33651524-33651543 None:intergenic 30.0%
AAAAGGGGTGAGATTTGTAA+AGG + chr2.1:33651256-33651275 MS.gene000185:CDS 35.0%
AAAATGAACATTATGGAGCC+AGG + chr2.1:33651335-33651354 MS.gene000185:CDS 35.0%
AGAAATCAGTCAGGATTCAT+TGG + chr2.1:33650811-33650830 MS.gene000185:CDS 35.0%
AGTGTTATCAGAAATCAGTC+AGG + chr2.1:33650802-33650821 MS.gene000185:CDS 35.0%
ATATGTGTGGCAGGATTTAA+TGG + chr2.1:33650364-33650383 MS.gene000185:CDS 35.0%
ATTTGTAAAGGAGACAAGCT+AGG + chr2.1:33651268-33651287 MS.gene000185:CDS 35.0%
CAACTGCATACGCAATTTAA+AGG - chr2.1:33651396-33651415 None:intergenic 35.0%
CAGTTTGTCCTAACATTTCA+TGG - chr2.1:33651885-33651904 None:intergenic 35.0%
CATGTCTATCAATGTTTGGT+AGG - chr2.1:33651981-33652000 None:intergenic 35.0%
GAATCCAATGCTACTATGAT+TGG + chr2.1:33651615-33651634 MS.gene000185:intron 35.0%
GACTATGACTATTCTAAGCA+TGG - chr2.1:33651535-33651554 None:intergenic 35.0%
GAGATGAAATAATCAAGAGC+AGG + chr2.1:33651746-33651765 MS.gene000185:CDS 35.0%
GAGTTTCTGCATTAGTACAA+AGG - chr2.1:33650625-33650644 None:intergenic 35.0%
GATAATGAATACGTCCTCAA+AGG + chr2.1:33650413-33650432 MS.gene000185:CDS 35.0%
GCTTCTAATCTTAGTGAATG+AGG - chr2.1:33651104-33651123 None:intergenic 35.0%
GGAGTAGAAAAAGTTGAATC+AGG - chr2.1:33651052-33651071 None:intergenic 35.0%
GTAGAAAAAGTTGAATCAGG+AGG - chr2.1:33651049-33651068 None:intergenic 35.0%
GTGTCATGTCTATCAATGTT+TGG - chr2.1:33651985-33652004 None:intergenic 35.0%
GTGTTGTCAATATCAGACAT+TGG + chr2.1:33652075-33652094 MS.gene000185:intron 35.0%
GTTGAAGTTGCAATTGCATT+CGG + chr2.1:33651450-33651469 MS.gene000185:intron 35.0%
TAACCCAATCATAGTAGCAT+TGG - chr2.1:33651622-33651641 None:intergenic 35.0%
TCAGTTACTTACGAGCAAAT+TGG - chr2.1:33651823-33651842 None:intergenic 35.0%
TTAAATTGCGTATGCAGTTG+CGG + chr2.1:33651396-33651415 MS.gene000185:intron 35.0%
TTCCAACAGAAAAGCCAATA+AGG + chr2.1:33652237-33652256 MS.gene000185:intron 35.0%
TTGTAAAGGAATGAAAGGCA+TGG + chr2.1:33652611-33652630 MS.gene000185:CDS 35.0%
! ATGTGTTTGTGCATTTTCTC+AGG - chr2.1:33652585-33652604 None:intergenic 35.0%
! GTTGAAGAAGAGAAGCTTTT+AGG + chr2.1:33650884-33650903 MS.gene000185:CDS 35.0%
! TGTAAAGGAATGAAAGGCAT+GGG + chr2.1:33652612-33652631 MS.gene000185:CDS 35.0%
!! CACAAACACCCTTTTAAGAA+TGG - chr2.1:33651596-33651615 None:intergenic 35.0%
!! CCGCAATATAAAGGTTTTAG+AGG + chr2.1:33649768-33649787 MS.gene000185:intron 35.0%
!! CTTTTAGGGAATGGAAAAGA+AGG + chr2.1:33650899-33650918 MS.gene000185:CDS 35.0%
!! TGAGAAAATGGACTAAAGCA+AGG - chr2.1:33650253-33650272 None:intergenic 35.0%
ACACAGTGTTGTCAAATAGC+TGG + chr2.1:33649993-33650012 MS.gene000185:intron 40.0%
CAAAAGGTGGAGAAGTTAGA+AGG + chr2.1:33649514-33649533 MS.gene000185:CDS 40.0%
CAATAAGGTCAAGATTCTGC+TGG + chr2.1:33652252-33652271 MS.gene000185:intron 40.0%
CACATTAATAGGCTCCTTAG+GGG - chr2.1:33651230-33651249 None:intergenic 40.0%
CAGCAGAATCTTGACCTTAT+TGG - chr2.1:33652254-33652273 None:intergenic 40.0%
CCACAATACAAACCATTTCG+AGG - chr2.1:33651485-33651504 None:intergenic 40.0%
GATCAGCAGTACTAAGATCA+GGG - chr2.1:33649689-33649708 None:intergenic 40.0%
GCACATTAATAGGCTCCTTA+GGG - chr2.1:33651231-33651250 None:intergenic 40.0%
GGTACACAACCGCAATATAA+AGG + chr2.1:33649759-33649778 MS.gene000185:intron 40.0%
GTTCTTCATCTTGCTCGAAA+CGG + chr2.1:33649600-33649619 MS.gene000185:CDS 40.0%
TACTTTCTTAGTCACATGGG+TGG + chr2.1:33649549-33649568 MS.gene000185:CDS 40.0%
TATCAGAAATGGGGGTTATG+AGG - chr2.1:33650397-33650416 None:intergenic 40.0%
TGAAGAACACGAATGAGATG+AGG - chr2.1:33649588-33649607 None:intergenic 40.0%
TGATCAGCAGTACTAAGATC+AGG - chr2.1:33649690-33649709 None:intergenic 40.0%
TGTGGATTTGTGATCCTTTG+AGG - chr2.1:33650430-33650449 None:intergenic 40.0%
TTACCAACTCTTGGTTGCAT+GGG + chr2.1:33652369-33652388 MS.gene000185:intron 40.0%
TTTACCAACTCTTGGTTGCA+TGG + chr2.1:33652368-33652387 MS.gene000185:intron 40.0%
! AACTATAGGAGGTACAAGAG+TGG + chr2.1:33650341-33650360 MS.gene000185:intron 40.0%
! AGTCATGACCCTTTTAGACA+TGG + chr2.1:33651551-33651570 MS.gene000185:intron 40.0%
! CCTCGAAATGGTTTGTATTG+TGG + chr2.1:33651482-33651501 MS.gene000185:intron 40.0%
! TGAAGCTGTACAAGTTGAAG+AGG + chr2.1:33650688-33650707 MS.gene000185:CDS 40.0%
!! CTAAAGTGTCGAAGCTACTT+AGG + chr2.1:33652143-33652162 MS.gene000185:intron 40.0%
!! GAAGAGAAGCTTTTAGGGAA+TGG + chr2.1:33650890-33650909 MS.gene000185:CDS 40.0%
!!! CAAATCTCACCCCTTTTTTC+TGG - chr2.1:33651253-33651272 None:intergenic 40.0%
!!! GACCTTATTGGCTTTTCTGT+TGG - chr2.1:33652242-33652261 None:intergenic 40.0%
!!! GGATTCATTGGTTTTCAGCT+CGG + chr2.1:33650823-33650842 MS.gene000185:CDS 40.0%
AACGCTAACACACACTGACA+CGG + chr2.1:33652107-33652126 MS.gene000185:intron 45.0%
AAGCTGTGTTTCCTCAGCAA+TGG - chr2.1:33652652-33652671 None:intergenic 45.0%
ACATACCAAAAGGGGTAGTG+TGG - chr2.1:33650448-33650467 None:intergenic 45.0%
ACCAAGCAAAGGATTCAGGT+AGG - chr2.1:33650205-33650224 None:intergenic 45.0%
ACCTACCTGAATCCTTTGCT+TGG + chr2.1:33650201-33650220 MS.gene000185:CDS 45.0%
CAACTGCATCAACCACATTG+TGG - chr2.1:33651434-33651453 None:intergenic 45.0%
CGAAACGGTGTTTATCTCAG+AGG + chr2.1:33649615-33649634 MS.gene000185:CDS 45.0%
GAGCAAATTGGTCCACCAAA+TGG - chr2.1:33651811-33651830 None:intergenic 45.0%
GGCACATTAATAGGCTCCTT+AGG - chr2.1:33651232-33651251 None:intergenic 45.0%
GGTACAAGAGTGGATATGTG+TGG + chr2.1:33650351-33650370 MS.gene000185:CDS 45.0%
TACGACCAAGCAAAGGATTC+AGG - chr2.1:33650209-33650228 None:intergenic 45.0%
TGACACCGATACTTACACGT+AGG + chr2.1:33651999-33652018 MS.gene000185:intron 45.0%
TGCTCAGAAAATTGCCCCTA+AGG + chr2.1:33651213-33651232 MS.gene000185:CDS 45.0%
TGGTGCCTACGTGTAAGTAT+CGG - chr2.1:33652007-33652026 None:intergenic 45.0%
TGTAAAGGAGACAAGCTAGG+AGG + chr2.1:33651271-33651290 MS.gene000185:CDS 45.0%
TTACACTGCGAACCACAATG+TGG + chr2.1:33651419-33651438 MS.gene000185:intron 45.0%
TTCAGCTACAACCATTGCTG+AGG + chr2.1:33652638-33652657 MS.gene000185:CDS 45.0%
TTGTATTGTGGCCGCAATTG+CGG + chr2.1:33651494-33651513 MS.gene000185:intron 45.0%
! CAAATCCACACTACCCCTTT+TGG + chr2.1:33650440-33650459 MS.gene000185:CDS 45.0%
! TAGGGGCAATTTTCTGAGCA+GGG - chr2.1:33651213-33651232 None:intergenic 45.0%
! TTAGGGGCAATTTTCTGAGC+AGG - chr2.1:33651214-33651233 None:intergenic 45.0%
!! AGTTGATGATGCAGGCAATG+TGG + chr2.1:33652546-33652565 MS.gene000185:intron 45.0%
!! GTTGATGATGCAGGCAATGT+GGG + chr2.1:33652547-33652566 MS.gene000185:intron 45.0%
!! TGAGACGATGTTGATGACGT+TGG - chr2.1:33651073-33651092 None:intergenic 45.0%
!! TTGGATTGGTTGTGGAACTG+TGG + chr2.1:33651156-33651175 MS.gene000185:CDS 45.0%
AAGTTGGGCAACCGCAATTG+CGG - chr2.1:33651508-33651527 None:intergenic 50.0%
ACAAGCTAGGAGGATCAGCT+AGG + chr2.1:33651281-33651300 MS.gene000185:CDS 50.0%
CAAGAGTGGATATGTGTGGC+AGG + chr2.1:33650355-33650374 MS.gene000185:CDS 50.0%
CCTCTTGTACGACCAAGCAA+AGG - chr2.1:33650216-33650235 None:intergenic 50.0%
CCTTTGCTTGGTCGTACAAG+AGG + chr2.1:33650213-33650232 MS.gene000185:CDS 50.0%
GAGGATCAGCTAGGTGCTTT+GGG + chr2.1:33651290-33651309 MS.gene000185:CDS 50.0%
TAGCCCATGCAACCAAGAGT+TGG - chr2.1:33652375-33652394 None:intergenic 50.0%
TTTCAGCTCGGACAGGTCAT+CGG + chr2.1:33650835-33650854 MS.gene000185:CDS 50.0%
!! AGATGGCTTGGTGAAGTGAG+AGG + chr2.1:33650173-33650192 MS.gene000185:CDS 50.0%
!! CTAGGTGCTTTGGGACTACT+TGG + chr2.1:33651299-33651318 MS.gene000185:CDS 50.0%
!!! CATTGGTTTTCAGCTCGGAC+AGG + chr2.1:33650828-33650847 MS.gene000185:CDS 50.0%
CGGTTGCAGATGCCTCGAAA+TGG + chr2.1:33651470-33651489 MS.gene000185:intron 55.0%
GGAGGATCAGCTAGGTGCTT+TGG + chr2.1:33651289-33651308 MS.gene000185:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 33649507 33652716 33649507 ID=MS.gene000185
chr2.1 mRNA 33649507 33652716 33649507 ID=MS.gene000185.t1;Parent=MS.gene000185
chr2.1 exon 33649507 33649636 33649507 ID=MS.gene000185.t1.exon1;Parent=MS.gene000185.t1
chr2.1 CDS 33649507 33649636 33649507 ID=cds.MS.gene000185.t1;Parent=MS.gene000185.t1
chr2.1 exon 33650165 33650234 33650165 ID=MS.gene000185.t1.exon2;Parent=MS.gene000185.t1
chr2.1 CDS 33650165 33650234 33650165 ID=cds.MS.gene000185.t1;Parent=MS.gene000185.t1
chr2.1 exon 33650349 33650461 33650349 ID=MS.gene000185.t1.exon3;Parent=MS.gene000185.t1
chr2.1 CDS 33650349 33650461 33650349 ID=cds.MS.gene000185.t1;Parent=MS.gene000185.t1
chr2.1 exon 33650639 33651356 33650639 ID=MS.gene000185.t1.exon4;Parent=MS.gene000185.t1
chr2.1 CDS 33650639 33651356 33650639 ID=cds.MS.gene000185.t1;Parent=MS.gene000185.t1
chr2.1 exon 33651732 33651830 33651732 ID=MS.gene000185.t1.exon5;Parent=MS.gene000185.t1
chr2.1 CDS 33651732 33651830 33651732 ID=cds.MS.gene000185.t1;Parent=MS.gene000185.t1
chr2.1 exon 33652560 33652716 33652560 ID=MS.gene000185.t1.exon6;Parent=MS.gene000185.t1
chr2.1 CDS 33652560 33652716 33652560 ID=cds.MS.gene000185.t1;Parent=MS.gene000185.t1
Gene Sequence

>MS.gene000185

ATGGAAACAAAAGGTGGAGAAGTTAGAAGGCTTCACATAATCTACTTTCTTAGTCACATGGGTGGTCATGCAGAACATCCTCATCTCATTCGTGTTCTTCATCTTGCTCGAAACGGTGTTTATCTCAGAGGTAAAACTCATTTATGTTCCATATTTTCATTCAATTTGTTCTGTACAGGCCCTGATCTTAGTACTGCTGATCATAATGTTTTTTCTTAAATGTGTATTCTGTCACACATAGTTCAAAATTGCGGTACACAACCGCAATATAAAGGTTTTAGAGGTTCACAATCATAACTGTAGCTGCATTTGTCAGCATTTTAGAACTATGCTGTCACAATTGCTTGTTTTGTCTGTTCTCGCTCTGACATTAGTACATCTTGTTTTTTCTGCTGCTCATAATGTCACTCACAATGTTGTCAAATAGCAGATATAGTGTCGTAGAATTTTAACAAATTGTTATCGTTCCGTGATACACAATTTAGTACACAGTGTTGTCAAATAGCTGGCTATTAGCAGAAGTTGAACAAACTCCTATTGTCTATGATCTGCAATTAACAACATTGTTAATCATAAATGTTTCATGATTATTAATTATTAATTTGTGACAATGATTTGTGAGTCTAAAATGACACTTTCTTTTTTATTTCTTGAAAAGATATTAAGAGATGGCTTGGTGAAGTGAGAGGAAGAGACCTACCTGAATCCTTTGCTTGGTCGTACAAGAGGTTGTTGAATCATTTCCTTGCTTTAGTCCATTTTCTCAACCTCATTTTTTTAACCTTTAACTATATATGTATCTCCATAATCAATTTTTGTAACTAACAATTATTGAACTATAGGAGGTACAAGAGTGGATATGTGTGGCAGGATTTAATGGATGACGACCTCATAACCCCCATTTCTGATAATGAATACGTCCTCAAAGGATCACAAATCCACACTACCCCTTTTGGTATGTACTTTATCATAATCACTACTATCCCATTTTTATTTATTCACATTAATGTTTTGAGTATGGTCACCACTAATTTCTATCTATCTACACTATTGCCTTATTATGTAATTTTTGCTAAGAATGTTAAACAAGAATAATTAAGTTGTACACTTTTTTTCCTTTGTACTAATGCAGAAACTCATGCAATTAATTCAACAATCAATGAAAAGAAAACTGATACTGATGAAGCTGTACAAGTTGAAGAGGACAAACAAAAGCAGCAAAAGAAACCATCTTTATCAGAAGAAGAATCTGAAATTCAGAAAGAAACTGATCCAAAAAAGAATGATCTAAATCAAGTGTTATCAGAAATCAGTCAGGATTCATTGGTTTTCAGCTCGGACAGGTCATCGGTGACTGACGATGATTCTTCTTTTAAAGTTGAAGAAGAGAAGCTTTTAGGGAATGGAAAAGAAGGTTGCTTGAATGACAAGAATCATGAGAAGCTTGATAGTTTCTCATTTCCTTCTTTGTATCATAATTTGATGAGTAAGAAGGGTAAAAAAGATGGTCATAATAACAAAACTGATACTACTACTACACCTCCTGATTCAACTTTTTCTACTCCAACGTCATCAACATCGTCTCAATCTCAATCCTCATTCACTAAGATTAGAAGCAATTCTATTAGAGTGACTAGTGTGTTCAAAAATTGGATTGGTTGTGGAACTGTGGCAACAAATGATGCTGCTTTTGTCTCTATGAACCCTGCTCAGAAAATTGCCCCTAAGGAGCCTATTAATGTGCCAGAAAAAAGGGGTGAGATTTGTAAAGGAGACAAGCTAGGAGGATCAGCTAGGTGCTTTGGGACTACTTGGAATTATCATGATCAAAATGAACATTATGGAGCCAGGTGAGAAATTGTATCATAAATTAGCTTAGAATCATGCCTTTAAATTGCGTATGCAGTTGCGGTTACACTGCGAACCACAATGTGGTTGATGCAGTTGAAGTTGCAATTGCATTCGGTTGCAGATGCCTCGAAATGGTTTGTATTGTGGCCGCAATTGCGGTTGCCCAACTTAAAACCATGCTTAGAATAGTCATAGTCATGACCCTTTTAGACATGGTTTTGAATCCTGTTAAAAACCATTCTTAAAAGGGTGTTTGTGAATCCAATGCTACTATGATTGGGTTACATTTACATTAGACATACGTCACTATGATTATTTGCAAAATTTAATGTCATGACACGTGACTAATAATAATTTCATGTTTCTTTTTACAGAAAAAGCTGTGATGGAGATGAAATAATCAAGAGCAGGAAAAAGCTAAGCGAGTTAATGAGTCAAACTTCTTATAAACCATTTGGTGGACCAATTTGCTCGTAAGTAACTGAATATGTCACTAGAGTAAACTTCAATTGTTTGGATTTCAACCATGAAATGTTAGGACAAACTGTGCATGAAAAATTTTGGTCTTATAGGTGGTAGATATTTTATGTAACACACAAGTTGAGTATCAATAGTGTCGACCTACCAAACATTGATAGACATGACACCGATACTTACACGTAGGCACCATTATTAAGTTGAAAAAATGGAAGTGGTCGAATGTAAACACATGTGTCTGTGTTGTCAATATCAGACATTGGCACATATCGAACGCTAACACACACTGACACGGACACTCTTTTAATCTAAAGTGTCGAAGCTACTTAGGTGTTGACTAGTTTGTTAGTTTTGCAATAAAAAACAAAACAAAAATATACTGAAACTACAATTGGTAAAAGTTTCCAACAGAAAAGCCAATAAGGTCAAGATTCTGCTGGTGTAATTATTATATTGTCAAAAATCAAAATAATGTTTTTTTCTTGTAAAAGAAATACAAAATATAAGTTGGCATTGATTGCTAGATTATGATGTTTACCAACTCTTGGTTGCATGGGCTAGATATACTTAGTAGCTGAATTTCTTGCATCTTAATTTAATGCATCAAAGTTAGAATAGTATGAGCTACATACTTTATATGCCCATATGTGTAAAGACACATTAAATTTTGTGTATTTAATTTTGATTATGAAAGATGAGACCATAAATAGTAGTTGATGATGCAGGCAATGTGGGAAGTCATTCAAGCCTGAGAAAATGCACAAACACATGAAGTCTTGTAAAGGAATGAAAGGCATGGGAACTTCAGCTACAACCATTGCTGAGGAAACACAGCTTCATAGATCAACTTCATCATCTAAAGATTACCTTTTGCAAAATTAA
Protein sequence

>MS.gene000185.t1

METKGGEVRRLHIIYFLSHMGGHAEHPHLIRVLHLARNGVYLRDIKRWLGEVRGRDLPESFAWSYKRRYKSGYVWQDLMDDDLITPISDNEYVLKGSQIHTTPFETHAINSTINEKKTDTDEAVQVEEDKQKQQKKPSLSEEESEIQKETDPKKNDLNQVLSEISQDSLVFSSDRSSVTDDDSSFKVEEEKLLGNGKEGCLNDKNHEKLDSFSFPSLYHNLMSKKGKKDGHNNKTDTTTTPPDSTFSTPTSSTSSQSQSSFTKIRSNSIRVTSVFKNWIGCGTVATNDAAFVSMNPAQKIAPKEPINVPEKRGEICKGDKLGGSARCFGTTWNYHDQNEHYGARKSCDGDEIIKSRKKLSELMSQTSYKPFGGPICSQCGKSFKPEKMHKHMKSCKGMKGMGTSATTIAEETQLHRSTSSSKDYLLQN