Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000268.t1 | TKY49529.1 | 39 | 77 | 47 | 0 | 32 | 108 | 129 | 205 | 7.00E-07 | 63.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene000268.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATCAAATTGAAATTAT+GGG | + | 43380:5337-5356 | MS.gene000268:intron | 10.0% |
| !! | AAAATCAAATTGAAATTATG+GGG | + | 43380:5338-5357 | MS.gene000268:intron | 15.0% |
| !! | CAAAAATCAAATTGAAATTA+TGG | + | 43380:5336-5355 | MS.gene000268:intron | 15.0% |
| !! | AAATCAAATTGAAATTATGG+GGG | + | 43380:5339-5358 | MS.gene000268:intron | 20.0% |
| !! | AAATTTAATATACATACCAG+AGG | - | 43380:5658-5677 | None:intergenic | 20.0% |
| !! | TCTGGTATGTATATTAAATT+TGG | + | 43380:5657-5676 | MS.gene000268:intron | 20.0% |
| !!! | ATTTAAAGTAGACAATTATC+AGG | - | 43380:5268-5287 | None:intergenic | 20.0% |
| !!! | GTAAATAAATCCATTCATTT+TGG | - | 43380:5395-5414 | None:intergenic | 20.0% |
| ! | AATCACATGAAAAAAAGTTG+TGG | + | 43380:5593-5612 | MS.gene000268:intron | 25.0% |
| ! | CATAAGAGTTGCAATTAAAA+GGG | + | 43380:5969-5988 | MS.gene000268:CDS | 25.0% |
| ! | GGATTTATTTACCTAAACAA+AGG | + | 43380:5403-5422 | MS.gene000268:intron | 25.0% |
| ! | GTATGTATATTAAATTTGGC+AGG | + | 43380:5661-5680 | MS.gene000268:intron | 25.0% |
| ! | TATTTACCTAAACAAAGGAT+GGG | + | 43380:5408-5427 | MS.gene000268:intron | 25.0% |
| ! | TTATTTACCTAAACAAAGGA+TGG | + | 43380:5407-5426 | MS.gene000268:intron | 25.0% |
| !! | AATAATTTTCGCAATGATAC+TGG | + | 43380:5556-5575 | MS.gene000268:intron | 25.0% |
| !! | ATAATTTTCGCAATGATACT+GGG | + | 43380:5557-5576 | MS.gene000268:intron | 25.0% |
| !!! | CAACTTTTTTTCATGTGATT+CGG | - | 43380:5593-5612 | None:intergenic | 25.0% |
| ATTGAAATTATGGGGGAAAA+TGG | + | 43380:5346-5365 | MS.gene000268:intron | 30.0% | |
| CATATATCATTTAGATGAGC+TGG | - | 43380:5304-5323 | None:intergenic | 30.0% | |
| CCATAAGAGTTGCAATTAAA+AGG | + | 43380:5968-5987 | MS.gene000268:CDS | 30.0% | |
| CCATTTCTTTCAGATTGTTT+CGG | - | 43380:5912-5931 | None:intergenic | 30.0% | |
| GATGCAGAAGTAATTATATC+AGG | + | 43380:5529-5548 | MS.gene000268:intron | 30.0% | |
| ! | AACTGGAGTTTTACAAAACA+GGG | + | 43380:5935-5954 | MS.gene000268:CDS | 30.0% |
| ! | AGCATTATTCTGTCTGAAAT+TGG | - | 43380:5145-5164 | None:intergenic | 30.0% |
| ! | TTTTCAGAATATTCCAGTTC+AGG | + | 43380:5744-5763 | MS.gene000268:intron | 30.0% |
| !! | CCTTTTAATTGCAACTCTTA+TGG | - | 43380:5971-5990 | None:intergenic | 30.0% |
| !!! | TTTTTTTCATGTGATTCGGT+TGG | - | 43380:5589-5608 | None:intergenic | 30.0% |
| ATTATGATCATGCTGCCAAA+TGG | + | 43380:5802-5821 | MS.gene000268:CDS | 35.0% | |
| CCGAAACAATCTGAAAGAAA+TGG | + | 43380:5909-5928 | MS.gene000268:CDS | 35.0% | |
| GACAATAATAAGTTGTGCTG+CGG | - | 43380:5185-5204 | None:intergenic | 35.0% | |
| GGAAAAGGATGGTATAAGTT+TGG | + | 43380:5883-5902 | MS.gene000268:CDS | 35.0% | |
| TCTGAAAGAAATGGATGAAC+TGG | + | 43380:5918-5937 | MS.gene000268:CDS | 35.0% | |
| TGAATATCCAGCAAGTTATC+TGG | + | 43380:5096-5115 | MS.gene000268:CDS | 35.0% | |
| TTATGATCATGCTGCCAAAT+GGG | + | 43380:5803-5822 | MS.gene000268:CDS | 35.0% | |
| ! | ACTTATACCATCCTTTTCCT+AGG | - | 43380:5882-5901 | None:intergenic | 35.0% |
| ! | ACTTGATGTTGTGAGTTTTG+TGG | - | 43380:5620-5639 | None:intergenic | 35.0% |
| ! | AGTTATCTGGAATGTCCAAA+AGG | + | 43380:5109-5128 | MS.gene000268:CDS | 35.0% |
| ! | ATTGGAGCAATAAACCCTTT+TGG | - | 43380:5127-5146 | None:intergenic | 35.0% |
| ! | GAACTGGAGTTTTACAAAAC+AGG | + | 43380:5934-5953 | MS.gene000268:CDS | 35.0% |
| ! | GGAAAATGGATTTATCTGGA+TGG | + | 43380:5360-5379 | MS.gene000268:intron | 35.0% |
| ! | GTTATCTGGAATGTCCAAAA+GGG | + | 43380:5110-5129 | MS.gene000268:CDS | 35.0% |
| ! | TGGCAGCATGATCATAATTT+TGG | - | 43380:5800-5819 | None:intergenic | 35.0% |
| ! | TTTCATCCCATCCTTTGTTT+AGG | - | 43380:5417-5436 | None:intergenic | 35.0% |
| !! | AGCATGATCATAATTTTGGC+TGG | - | 43380:5796-5815 | None:intergenic | 35.0% |
| !!! | CATAATTTTGGCTGGTTTGT+CGG | - | 43380:5788-5807 | None:intergenic | 35.0% |
| CAGAATAATGCTACACACAC+TGG | + | 43380:5153-5172 | MS.gene000268:CDS | 40.0% | |
| GATTTATCTGGATGGTTGAG+TGG | + | 43380:5368-5387 | MS.gene000268:intron | 40.0% | |
| GTTGAGTGGACCAAAATGAA+TGG | + | 43380:5382-5401 | MS.gene000268:intron | 40.0% | |
| TATGATCATGCTGCCAAATG+GGG | + | 43380:5804-5823 | MS.gene000268:CDS | 40.0% | |
| TCAGAATATTCCAGTTCAGG+TGG | + | 43380:5747-5766 | MS.gene000268:intron | 40.0% | |
| ! | TGGGGGAAAATGGATTTATC+TGG | + | 43380:5356-5375 | MS.gene000268:intron | 40.0% |
| AAACGTTACGAATGTCGCCT+AGG | + | 43380:5862-5881 | MS.gene000268:CDS | 45.0% | |
| AATGTCGCCTAGGAAAAGGA+TGG | + | 43380:5872-5891 | MS.gene000268:CDS | 45.0% | |
| ACATACCAGAGGTTGTCTTC+CGG | - | 43380:5647-5666 | None:intergenic | 45.0% | |
| CAAATGGGGATGTGGAAGAA+TGG | + | 43380:5818-5837 | MS.gene000268:CDS | 45.0% | |
| CATTCTTCCACATCCCCATT+TGG | - | 43380:5820-5839 | None:intergenic | 45.0% | |
| CTCACAACATCAAGTTCCAC+CGG | + | 43380:5625-5644 | MS.gene000268:intron | 45.0% | |
| TACGAATGTCGCCTAGGAAA+AGG | + | 43380:5868-5887 | MS.gene000268:CDS | 45.0% | |
| ! | AAACGTTTTGCCACCTGAAC+TGG | - | 43380:5760-5779 | None:intergenic | 45.0% |
| ! | GGACATTCCAGATAACTTGC+TGG | - | 43380:5106-5125 | None:intergenic | 45.0% |
| CATGCTGCCAAATGGGGATG+TGG | + | 43380:5810-5829 | MS.gene000268:CDS | 55.0% | |
| TACCAGAGGTTGTCTTCCGG+TGG | - | 43380:5644-5663 | None:intergenic | 55.0% | |
| TTCCACCGGAAGACAACCTC+TGG | + | 43380:5639-5658 | MS.gene000268:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43380 | gene | 5085 | 6002 | 5085 | ID=MS.gene000268 |
| 43380 | mRNA | 5085 | 6002 | 5085 | ID=MS.gene000268.t1;Parent=MS.gene000268 |
| 43380 | exon | 5085 | 5174 | 5085 | ID=MS.gene000268.t1.exon1;Parent=MS.gene000268.t1 |
| 43380 | CDS | 5085 | 5174 | 5085 | ID=cds.MS.gene000268.t1;Parent=MS.gene000268.t1 |
| 43380 | exon | 5751 | 6002 | 5751 | ID=MS.gene000268.t1.exon2;Parent=MS.gene000268.t1 |
| 43380 | CDS | 5751 | 6002 | 5751 | ID=cds.MS.gene000268.t1;Parent=MS.gene000268.t1 |
| Gene Sequence |
| Protein sequence |
