AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000298

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000298.t1 MTR_8g017210 65.385 156 53 1 29 184 62 216 2.85e-74 243
MS.gene000298.t1 MTR_1g016010 61.988 171 58 3 21 185 28 197 4.74e-73 238
MS.gene000298.t1 MTR_2g099990 63.462 156 56 1 29 184 46 200 2.14e-72 236
MS.gene000298.t1 MTR_1g094115 62.179 156 58 1 29 184 47 201 1.94e-71 236
MS.gene000298.t1 MTR_2g093960 61.538 156 58 2 29 183 32 186 6.40e-70 228
MS.gene000298.t1 MTR_1g112270 61.438 153 58 1 32 184 47 198 1.10e-69 232
MS.gene000298.t1 MTR_4g059870 61.783 157 58 2 29 184 38 193 2.07e-69 229
MS.gene000298.t1 MTR_1g112270 62.069 145 54 1 40 184 66 209 1.58e-66 224
MS.gene000298.t1 MTR_2g090745 63.514 148 53 1 37 184 15 161 1.24e-65 218
MS.gene000298.t1 MTR_2g099990 63.636 132 47 1 53 184 1 131 4.72e-60 202
MS.gene000298.t1 MTR_1g094115 54.487 156 53 2 29 184 47 184 7.18e-57 197
MS.gene000298.t1 MTR_2g017720 60.000 115 40 1 39 153 323 431 3.30e-40 150
MS.gene000298.t1 MTR_3g102000 47.015 134 60 4 25 151 2 131 8.93e-28 110
MS.gene000298.t1 MTR_5g030560 64.444 45 16 0 39 83 16 60 2.87e-16 73.9
MS.gene000298.t1 MTR_4g103250 39.241 79 48 0 360 438 62 140 4.83e-16 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000298.t1 AT1G68130 48.458 454 139 10 22 457 34 410 5.32e-128 378
MS.gene000298.t1 AT2G01940 82.126 207 28 4 21 218 32 238 6.24e-123 366
MS.gene000298.t1 AT2G01940 80.383 209 29 6 21 218 32 239 1.48e-117 352
MS.gene000298.t1 AT1G25250 81.026 195 32 2 22 211 26 220 1.24e-113 340
MS.gene000298.t1 AT1G25250 43.704 135 69 2 329 457 249 382 1.08e-22 100
MS.gene000298.t1 AT1G25250 81.461 178 28 2 39 211 20 197 9.34e-103 311
MS.gene000298.t1 AT1G25250 43.704 135 69 2 329 457 226 359 7.75e-23 100
MS.gene000298.t1 AT1G68130 42.893 401 134 10 75 457 1 324 1.97e-88 273
MS.gene000298.t1 AT2G01940 80.667 150 24 3 75 219 1 150 1.11e-83 262
MS.gene000298.t1 AT1G25250 77.465 142 27 2 75 211 1 142 8.48e-74 235
MS.gene000298.t1 AT1G25250 43.704 135 69 2 329 457 171 304 3.14e-23 100
MS.gene000298.t1 AT5G66730 64.151 159 56 1 26 184 29 186 1.95e-72 237
MS.gene000298.t1 AT3G50700 59.195 174 65 2 26 194 31 203 3.51e-72 235
MS.gene000298.t1 AT1G55110 62.500 160 58 2 26 184 60 218 1.02e-70 231
MS.gene000298.t1 AT1G55110 62.500 160 58 2 26 184 60 218 1.02e-70 231
MS.gene000298.t1 AT1G55110 62.500 160 58 2 26 184 60 218 1.02e-70 231
MS.gene000298.t1 AT2G02080 60.256 156 61 1 29 184 54 208 1.86e-70 233
MS.gene000298.t1 AT2G02080 60.256 156 61 1 29 184 54 208 1.86e-70 233
MS.gene000298.t1 AT2G02080 60.256 156 61 1 29 184 54 208 1.86e-70 233
MS.gene000298.t1 AT2G02080 60.256 156 61 1 29 184 54 208 1.86e-70 233
MS.gene000298.t1 AT1G03840 63.462 156 56 1 29 184 41 195 4.84e-70 231
MS.gene000298.t1 AT5G03150 61.491 161 60 2 25 184 49 208 5.01e-70 231
MS.gene000298.t1 AT4G02670 58.757 177 66 3 29 199 55 230 2.34e-69 226
MS.gene000298.t1 AT4G02670 58.757 177 66 3 29 199 53 228 2.39e-69 226
MS.gene000298.t1 AT3G13810 61.875 160 59 2 25 183 47 205 1.08e-68 228
MS.gene000298.t1 AT1G14580 58.333 156 64 1 29 184 53 207 1.09e-68 227
MS.gene000298.t1 AT1G14580 58.333 156 64 1 29 184 53 207 1.09e-68 227
MS.gene000298.t1 AT3G13810 61.875 160 59 2 25 183 66 224 1.24e-68 228
MS.gene000298.t1 AT3G13810 61.875 160 59 2 25 183 66 224 1.24e-68 228
MS.gene000298.t1 AT3G13810 61.875 160 59 2 25 183 61 219 1.39e-68 228
MS.gene000298.t1 AT1G14580 58.333 156 64 1 29 184 67 221 1.61e-68 226
MS.gene000298.t1 AT5G44160 62.179 156 58 1 29 184 37 191 4.89e-68 225
MS.gene000298.t1 AT5G60470 50.000 204 95 3 6 208 26 223 1.25e-67 224
MS.gene000298.t1 AT5G60470 50.000 204 95 3 6 208 22 219 1.32e-67 223
MS.gene000298.t1 AT2G02070 59.615 156 62 1 29 184 52 206 3.63e-67 226
MS.gene000298.t1 AT2G02070 59.615 156 62 1 29 184 52 206 3.63e-67 226
MS.gene000298.t1 AT3G45260 59.873 157 61 2 29 184 39 194 4.13e-67 222
MS.gene000298.t1 AT3G45260 59.873 157 61 2 29 184 39 194 4.13e-67 222
MS.gene000298.t1 AT1G03840 62.179 156 56 2 29 184 41 193 2.30e-66 221
MS.gene000298.t1 AT5G44160 63.014 146 53 1 39 184 8 152 1.58e-64 214
MS.gene000298.t1 AT3G13810 63.448 145 51 2 40 183 68 211 1.53e-63 214
MS.gene000298.t1 AT3G13810 63.448 145 51 2 40 183 82 225 2.32e-63 214
MS.gene000298.t1 AT5G60470 53.529 170 77 2 40 208 46 214 2.16e-61 207
MS.gene000298.t1 AT2G02080 59.091 132 53 1 53 184 1 131 6.49e-58 197
MS.gene000298.t1 AT2G02080 59.091 132 53 1 53 184 1 131 6.49e-58 197
MS.gene000298.t1 AT5G22890 31.214 173 99 6 24 179 182 351 8.31e-18 85.5
MS.gene000298.t1 AT1G34370 31.210 157 91 5 38 179 14 168 1.11e-16 80.9
MS.gene000298.t1 AT1G34370 31.210 157 91 5 38 179 224 378 1.72e-16 82.4
MS.gene000298.t1 AT1G34370 31.210 157 91 5 38 179 224 378 1.72e-16 82.4
MS.gene000298.t1 AT3G48550 41.772 79 46 0 360 438 79 157 8.51e-16 75.1
MS.gene000298.t1 AT3G20880 29.252 147 94 4 50 188 248 392 9.37e-12 67.0

Find 81 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTATGCATCTGTAAATTT+TGG 0.191998 6.4:-49918073 None:intergenic
ATAGTAGTAATAGGAGTAAT+AGG 0.205036 6.4:-49919893 None:intergenic
TTGAAATTTGTTTCACTTGA+TGG 0.265033 6.4:-49919800 None:intergenic
GCACTTCTTCCACTAATAAA+AGG 0.270599 6.4:+49917589 MS.gene000298:CDS
AGTGAACTTGATAAAGCTTA+TGG 0.272526 6.4:+49920379 MS.gene000298:CDS
CATGATCCTTGTCATGCTTT+AGG 0.279693 6.4:+49918188 MS.gene000298:CDS
GAAGGTGTAATAGTAGTAAT+AGG 0.296600 6.4:-49919902 None:intergenic
ACAAGCAACGGAACAATTAA+AGG 0.306652 6.4:+49920243 MS.gene000298:CDS
GAGTAGAAGTTTATTGTTCT+TGG 0.315722 6.4:-49919939 None:intergenic
TCAACATGAAGATAACTCTT+TGG 0.326169 6.4:+49920477 MS.gene000298:CDS
ACTCAGTTGCATCTCTCAAT+AGG 0.329653 6.4:+49920082 MS.gene000298:CDS
GAGAGTTGAAGTTCCAAGTT+TGG 0.344241 6.4:-49919965 None:intergenic
TTGCTATTGCTACTCTCCTT+TGG 0.344778 6.4:-49920166 None:intergenic
TTTGGTCCCTTTGGAATCCT+TGG 0.356941 6.4:-49918055 None:intergenic
TTGGAGAAAGTTTGAGAAGA+AGG 0.371907 6.4:-49919920 None:intergenic
TTCACCACCTTCAGTAGTTA+TGG 0.411636 6.4:-49920521 None:intergenic
GTGGTTGAGATTCTGAATTA+TGG 0.414564 6.4:-49919730 None:intergenic
AGAGACACCACTTCTGCATC+TGG 0.419684 6.4:-49917981 None:intergenic
TCTCTCTTCAATAGCTTCCA+TGG 0.428102 6.4:-49918110 None:intergenic
AGATTGCACTGCATAACCTT+TGG 0.429152 6.4:-49918285 None:intergenic
AGCAAGGAAACAAATTGAAA+TGG 0.432424 6.4:+49920309 MS.gene000298:CDS
TGGTGCAAACAAGTTGGTTC+AGG 0.440705 6.4:-49918167 None:intergenic
AGAAGACAAACCTGGTGTTC+CGG 0.442410 6.4:-49917625 None:intergenic
GTAGAAAATGTTGATGGAAA+AGG 0.444254 6.4:+49920544 MS.gene000298:CDS
CTCAGTTGCATCTCTCAATA+GGG 0.455582 6.4:+49920083 MS.gene000298:CDS
CGTGTTGGTGTTGTTTGTGG+TGG 0.457194 6.4:-49919987 None:intergenic
CATGCTTTAGGTGATCTTGT+TGG 0.466169 6.4:+49918200 MS.gene000298:CDS
TTTGTTTCACTTGATGGACT+TGG 0.468237 6.4:-49919794 None:intergenic
ACTTCTACTCCATCCAAACT+TGG 0.469640 6.4:+49919952 MS.gene000298:CDS
GTACCAACACTTCTGATTGA+AGG 0.475892 6.4:-49920028 None:intergenic
TCAGAAGATGGAAGTGAAGA+TGG 0.478640 6.4:-49917543 None:intergenic
AAACAACATGGCTTTGTTGA+GGG 0.481856 6.4:+49920213 MS.gene000298:CDS
AGTCGTGTTGGTGTTGTTTG+TGG 0.482657 6.4:-49919990 None:intergenic
TAGTAGTAATAGGAGTAATA+GGG 0.486179 6.4:-49919892 None:intergenic
AGGCTATTCAGAAGGTTAAC+CGG 0.501979 6.4:+49920056 MS.gene000298:CDS
ACATAGTAATCATAAACAAT+GGG 0.505280 6.4:+49918247 MS.gene000298:CDS
AGAAGACCGGCCGGAACACC+AGG 0.510779 6.4:+49917615 MS.gene000298:CDS
CATCAAGATGCTTGCAACAT+GGG 0.511025 6.4:+49919701 MS.gene000298:CDS
GTTGAAGTTCCAAGTTTGGA+TGG 0.511669 6.4:-49919961 None:intergenic
GGTGAAGTAGAAAATGTTGA+TGG 0.512722 6.4:+49920538 MS.gene000298:CDS
GTATGTGAGATCTGCAACCA+AGG 0.515942 6.4:+49918038 MS.gene000298:CDS
CAAACAACATGGCTTTGTTG+AGG 0.518950 6.4:+49920212 MS.gene000298:CDS
GGAACAATTAAAGGTAGCTA+TGG 0.527664 6.4:+49920252 MS.gene000298:CDS
AACATAGTAATCATAAACAA+TGG 0.531879 6.4:+49918246 MS.gene000298:CDS
CTAAAGCATGACAAGGATCA+TGG 0.532248 6.4:-49918187 None:intergenic
ATTTACAGATGCATAGAAGA+AGG 0.537823 6.4:+49918078 MS.gene000298:CDS
GTCTGTTGTCAGAGTCGTGT+TGG 0.543599 6.4:-49920002 None:intergenic
GATGCATAGAAGAAGGCATA+AGG 0.546337 6.4:+49918085 MS.gene000298:CDS
TCTTCTGCCATAACTACTGA+AGG 0.549896 6.4:+49920514 MS.gene000298:CDS
GCTTGAAGCCTTGTTTGCCA+TGG 0.551999 6.4:-49919830 None:intergenic
GCATCAAGATGCTTGCAACA+TGG 0.560086 6.4:+49919700 MS.gene000298:CDS
GACAAACCTGGTGTTCCGGC+CGG 0.564975 6.4:-49917621 None:intergenic
AGAGATGCAACTGAGTTGAC+CGG 0.567581 6.4:-49920075 None:intergenic
AAGATGCTTGCAACATGGGA+AGG 0.571020 6.4:+49919705 MS.gene000298:CDS
CTCAAAACTTGTGGTACTAG+AGG 0.574559 6.4:+49918323 MS.gene000298:CDS
ATACATGTCCATGGCAAACA+AGG 0.574678 6.4:+49919822 MS.gene000298:CDS
AGATAAGCTATTAGCTAACA+TGG 0.580180 6.4:-49917520 None:intergenic
TACTGTGGAGGCTATTCAGA+AGG 0.581212 6.4:+49920048 MS.gene000298:CDS
CATGCAGTTGGTGTTTGAAG+TGG 0.583027 6.4:-49919749 None:intergenic
CAAATTTCAATACATGTCCA+TGG 0.586561 6.4:+49919813 MS.gene000298:CDS
AAAGCTCATCTCAAAACTTG+TGG 0.590538 6.4:+49918314 MS.gene000298:CDS
AGAGGTCATTCATGTGATTG+TGG 0.593681 6.4:+49918341 MS.gene000298:CDS
AAGCTTATGCTGAAGAAGCA+AGG 0.595645 6.4:+49920281 MS.gene000298:CDS
AATCAGAAGTGTTGGTACTG+TGG 0.596959 6.4:+49920033 MS.gene000298:CDS
TAATAAAAGGAAAAGAAGAC+CGG 0.599740 6.4:+49917602 MS.gene000298:CDS
GTTTGTGAAAGATGTTCCAA+AGG 0.605307 6.4:+49918269 MS.gene000298:CDS
GTTCTTGACAAGCATGCAGT+TGG 0.605757 6.4:-49919761 None:intergenic
CTGCAACCAAGGATTCCAAA+GGG 0.607708 6.4:+49918049 MS.gene000298:CDS
GGATCATGGTGCAAACAAGT+TGG 0.608116 6.4:-49918173 None:intergenic
AAAAGGAAAAGAAGACCGGC+CGG 0.608705 6.4:+49917606 MS.gene000298:CDS
ACGCCTTCAATCAGAAGTGT+TGG 0.611782 6.4:+49920025 MS.gene000298:CDS
GAAGAAGGCATAAGGTACCA+TGG 0.614434 6.4:+49918093 MS.gene000298:CDS
AAGAAGCAAGGAAACAAGCA+AGG 0.615543 6.4:+49920293 MS.gene000298:CDS
AATAGATCCAGATGCAGAAG+TGG 0.615906 6.4:+49917974 MS.gene000298:intron
CAGAAGTGTTGGTACTGTGG+AGG 0.634164 6.4:+49920036 MS.gene000298:CDS
AAACTCAAACTCATCACCAA+AGG 0.637316 6.4:+49920150 MS.gene000298:CDS
TCTGCAACCAAGGATTCCAA+AGG 0.641443 6.4:+49918048 MS.gene000298:CDS
GAGGGTTCAAGAACAAGCAA+CGG 0.651961 6.4:+49920231 MS.gene000298:CDS
TCTGCCATAACTACTGAAGG+TGG 0.661155 6.4:+49920517 MS.gene000298:CDS
ATCAACAACAACAAACAACA+TGG 0.678900 6.4:+49920201 MS.gene000298:CDS
AGATCACCTAAAGCATGACA+AGG 0.685132 6.4:-49918194 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAAAATAATATTAA+TGG - chr6.4:49919044-49919063 None:intergenic 0.0%
!! CTACAAAAAATATATATAAA+TGG - chr6.4:49919662-49919681 None:intergenic 10.0%
!! TATAAAAAAATACTTGAAAA+AGG - chr6.4:49918453-49918472 None:intergenic 10.0%
!!! CATTTATATATATTTTTTGT+AGG + chr6.4:49919660-49919679 MS.gene000298:intron 10.0%
!!! TGTTATATTTTTATTTTGTT+TGG + chr6.4:49917946-49917965 MS.gene000298:intron 10.0%
!!! TTTGAAACAAAAATATAAAA+AGG - chr6.4:49919326-49919345 None:intergenic 10.0%
!! AAAACATAAAGAAATGTTTA+AGG - chr6.4:49918995-49919014 None:intergenic 15.0%
!! AATATAAAAGTAAGGAAAAT+GGG + chr6.4:49918734-49918753 MS.gene000298:intron 15.0%
!! AATCTTTATATATACTTCAT+GGG + chr6.4:49918570-49918589 MS.gene000298:intron 15.0%
!! GTCAATTATTATTGTTAATT+AGG + chr6.4:49919469-49919488 MS.gene000298:intron 15.0%
!! TATTACTATTTAAAGAATTG+AGG - chr6.4:49919562-49919581 None:intergenic 15.0%
!!! TATTTTTATTTTGTTTGGTT+TGG + chr6.4:49917951-49917970 MS.gene000298:intron 15.0%
!! AAAAAACATGATTCAAGAAT+TGG - chr6.4:49917738-49917757 None:intergenic 20.0%
!! AACATAGTAATCATAAACAA+TGG + chr6.4:49918246-49918265 MS.gene000298:CDS 20.0%
!! ACATAGTAATCATAAACAAT+GGG + chr6.4:49918247-49918266 MS.gene000298:CDS 20.0%
!! ATGATGATTATAATGAATTG+AGG + chr6.4:49918648-49918667 MS.gene000298:intron 20.0%
!! GAATATAAAAGTAAGGAAAA+TGG + chr6.4:49918733-49918752 MS.gene000298:intron 20.0%
!! GAATCTTTATATATACTTCA+TGG + chr6.4:49918569-49918588 MS.gene000298:intron 20.0%
!! GTAAATTTACATATACTAGT+AGG + chr6.4:49919609-49919628 MS.gene000298:intron 20.0%
!! TAATAATAAAATCACACACA+TGG - chr6.4:49919417-49919436 None:intergenic 20.0%
!! TCAAAAATATATTTGACTGA+TGG + chr6.4:49918798-49918817 MS.gene000298:intron 20.0%
!! TTCATTAGATATAAAGAGTT+AGG - chr6.4:49919301-49919320 None:intergenic 20.0%
!!! AAAAAGGAAGATATGTTTTT+TGG + chr6.4:49918706-49918725 MS.gene000298:intron 20.0%
!!! AAAACTCTTTTTCTAACAAT+TGG - chr6.4:49918140-49918159 None:intergenic 20.0%
!!! TCATGTTTTTTTTGTTGTAT+TGG + chr6.4:49917747-49917766 MS.gene000298:intron 20.0%
!!! TTTTGTCCTTTTTATTTTAG+TGG + chr6.4:49919144-49919163 MS.gene000298:intron 20.0%
!!! TTTTTGAAGAAAACACAAAA+CGG - chr6.4:49918933-49918952 None:intergenic 20.0%
! AAAAATGTACAAAGGAAGAA+AGG - chr6.4:49919277-49919296 None:intergenic 25.0%
! AATGAAAAAGTGGATGTAAA+AGG - chr6.4:49919236-49919255 None:intergenic 25.0%
! AGTTAGGAAAAAATGTACAA+AGG - chr6.4:49919285-49919304 None:intergenic 25.0%
! ATAGTAGTAATAGGAGTAAT+AGG - chr6.4:49919896-49919915 None:intergenic 25.0%
! ATGAATCAAGAATCAATGAT+AGG - chr6.4:49918498-49918517 None:intergenic 25.0%
! CAGTTTCCACTAAAATAAAA+AGG - chr6.4:49919153-49919172 None:intergenic 25.0%
! TAATAAAAGGAAAAGAAGAC+CGG + chr6.4:49917602-49917621 MS.gene000298:CDS 25.0%
! TAGTAGTAATAGGAGTAATA+GGG - chr6.4:49919895-49919914 None:intergenic 25.0%
! TTATATATACTTCATGGGAT+GGG + chr6.4:49918575-49918594 MS.gene000298:intron 25.0%
! TTTATATATACTTCATGGGA+TGG + chr6.4:49918574-49918593 MS.gene000298:intron 25.0%
! TTTGTGTAGAAAGAATGAAA+TGG - chr6.4:49918964-49918983 None:intergenic 25.0%
!! TCTTCTTTTCCTTTTATTAG+TGG - chr6.4:49917601-49917620 None:intergenic 25.0%
!! TTCTATGCATCTGTAAATTT+TGG - chr6.4:49918076-49918095 None:intergenic 25.0%
!! TTGAAATTTGTTTCACTTGA+TGG - chr6.4:49919803-49919822 None:intergenic 25.0%
!! TTGGGATTTGACTTTAAAAA+AGG + chr6.4:49918690-49918709 MS.gene000298:intron 25.0%
!!! AAAACTTGAGTTGTTTTACT+TGG + chr6.4:49918671-49918690 MS.gene000298:intron 25.0%
!!! AAACTTGAGTTGTTTTACTT+GGG + chr6.4:49918672-49918691 MS.gene000298:intron 25.0%
!!! CTTTTTATTTTAGTGGAAAC+TGG + chr6.4:49919151-49919170 MS.gene000298:intron 25.0%
!!! GTTGATTCTTTTGTTGTTTT+TGG - chr6.4:49919860-49919879 None:intergenic 25.0%
AAAAAGAAAGAGGATTTAGG+AGG - chr6.4:49917654-49917673 None:intergenic 30.0%
AAAAGTAAGGAAAATGGGAA+TGG + chr6.4:49918739-49918758 MS.gene000298:intron 30.0%
AAGAGAGACAAATGAAAAAG+TGG - chr6.4:49919246-49919265 None:intergenic 30.0%
AATGGGAATGGAAAAACAAA+AGG + chr6.4:49918751-49918770 MS.gene000298:intron 30.0%
AGCAAGGAAACAAATTGAAA+TGG + chr6.4:49920309-49920328 MS.gene000298:CDS 30.0%
AGTGAACTTGATAAAGCTTA+TGG + chr6.4:49920379-49920398 MS.gene000298:CDS 30.0%
ATCAACAACAACAAACAACA+TGG + chr6.4:49920201-49920220 MS.gene000298:CDS 30.0%
ATTTACAGATGCATAGAAGA+AGG + chr6.4:49918078-49918097 MS.gene000298:CDS 30.0%
CAAATTTCAATACATGTCCA+TGG + chr6.4:49919813-49919832 MS.gene000298:CDS 30.0%
GAGTAGAAGTTTATTGTTCT+TGG - chr6.4:49919942-49919961 None:intergenic 30.0%
TCAACATGAAGATAACTCTT+TGG + chr6.4:49920477-49920496 MS.gene000298:CDS 30.0%
TGGAAGGGAATATAAAAGTA+AGG + chr6.4:49918726-49918745 MS.gene000298:intron 30.0%
! CAATTCAGCTTTATGTTTCT+GGG + chr6.4:49919533-49919552 MS.gene000298:intron 30.0%
! CCATTTTCATTGCAAGAAAA+AGG - chr6.4:49917570-49917589 None:intergenic 30.0%
! CCTTTTTCTTGCAATGAAAA+TGG + chr6.4:49917567-49917586 MS.gene000298:CDS 30.0%
! GAAGGTGTAATAGTAGTAAT+AGG - chr6.4:49919905-49919924 None:intergenic 30.0%
! GTAGAAAATGTTGATGGAAA+AGG + chr6.4:49920544-49920563 MS.gene000298:CDS 30.0%
! TCTGATTCCAACAATGTTTT+TGG - chr6.4:49918011-49918030 None:intergenic 30.0%
!!! AGGAAGATATGTTTTTTGGA+AGG + chr6.4:49918710-49918729 MS.gene000298:intron 30.0%
!!! GGAAGATATGTTTTTTGGAA+GGG + chr6.4:49918711-49918730 MS.gene000298:intron 30.0%
AAACTCAAACTCATCACCAA+AGG + chr6.4:49920150-49920169 MS.gene000298:CDS 35.0%
AAAGCTCATCTCAAAACTTG+TGG + chr6.4:49918314-49918333 MS.gene000298:CDS 35.0%
ACAAGCAACGGAACAATTAA+AGG + chr6.4:49920243-49920262 MS.gene000298:CDS 35.0%
CAAAACGGTTATAAGAGCAA+CGG - chr6.4:49918918-49918937 None:intergenic 35.0%
GCACTTCTTCCACTAATAAA+AGG + chr6.4:49917589-49917608 MS.gene000298:CDS 35.0%
GCGAAAAAGAAAGAGGATTT+AGG - chr6.4:49917657-49917676 None:intergenic 35.0%
GGAACAATTAAAGGTAGCTA+TGG + chr6.4:49920252-49920271 MS.gene000298:CDS 35.0%
GGTGAAGTAGAAAATGTTGA+TGG + chr6.4:49920538-49920557 MS.gene000298:CDS 35.0%
GTGGTTGAGATTCTGAATTA+TGG - chr6.4:49919733-49919752 None:intergenic 35.0%
GTTATAAGAGCAACGGAAAA+CGG - chr6.4:49918911-49918930 None:intergenic 35.0%
GTTTGTGAAAGATGTTCCAA+AGG + chr6.4:49918269-49918288 MS.gene000298:CDS 35.0%
TCTCTCACCAAAAACATTGT+TGG + chr6.4:49918001-49918020 MS.gene000298:CDS 35.0%
TTGGAGAAAGTTTGAGAAGA+AGG - chr6.4:49919923-49919942 None:intergenic 35.0%
! AAAGTGGATGTAAAAGGTGT+TGG - chr6.4:49919230-49919249 None:intergenic 35.0%
! GCAATTCAGCTTTATGTTTC+TGG + chr6.4:49919532-49919551 MS.gene000298:intron 35.0%
! GTTAGAGTTTAGAAGTACGA+AGG - chr6.4:49917694-49917713 None:intergenic 35.0%
! GTTGAGATTTTCTCTAACAC+TGG - chr6.4:49917716-49917735 None:intergenic 35.0%
! TATCCACTGGATTTTCCAAA+TGG - chr6.4:49919197-49919216 None:intergenic 35.0%
!! AAACAACATGGCTTTGTTGA+GGG + chr6.4:49920213-49920232 MS.gene000298:CDS 35.0%
!! GATGTAAAAGGTGTTGGAAA+AGG - chr6.4:49919224-49919243 None:intergenic 35.0%
!! TCTGTAAATTTTGGTCCCTT+TGG - chr6.4:49918067-49918086 None:intergenic 35.0%
!! TTTGTTTCACTTGATGGACT+TGG - chr6.4:49919797-49919816 None:intergenic 35.0%
AAGAAGCAAGGAAACAAGCA+AGG + chr6.4:49920293-49920312 MS.gene000298:CDS 40.0%
AAGCTTATGCTGAAGAAGCA+AGG + chr6.4:49920281-49920300 MS.gene000298:CDS 40.0%
AATAGATCCAGATGCAGAAG+TGG + chr6.4:49917974-49917993 MS.gene000298:intron 40.0%
AATCACACACATGGATGAAG+AGG - chr6.4:49919408-49919427 None:intergenic 40.0%
ACTCAGTTGCATCTCTCAAT+AGG + chr6.4:49920082-49920101 MS.gene000298:CDS 40.0%
ACTTCTACTCCATCCAAACT+TGG + chr6.4:49919952-49919971 MS.gene000298:CDS 40.0%
AGAGGTCATTCATGTGATTG+TGG + chr6.4:49918341-49918360 MS.gene000298:CDS 40.0%
AGATCACCTAAAGCATGACA+AGG - chr6.4:49918197-49918216 None:intergenic 40.0%
AGATTGCACTGCATAACCTT+TGG - chr6.4:49918288-49918307 None:intergenic 40.0%
AGGCTATTCAGAAGGTTAAC+CGG + chr6.4:49920056-49920075 MS.gene000298:CDS 40.0%
ATACATGTCCATGGCAAACA+AGG + chr6.4:49919822-49919841 MS.gene000298:CDS 40.0%
CAAGAAAAAGGCTCAGAAGA+TGG - chr6.4:49917558-49917577 None:intergenic 40.0%
CATCAAGATGCTTGCAACAT+GGG + chr6.4:49919701-49919720 MS.gene000298:CDS 40.0%
CATGATCCTTGTCATGCTTT+AGG + chr6.4:49918188-49918207 MS.gene000298:CDS 40.0%
CTAAAGCATGACAAGGATCA+TGG - chr6.4:49918190-49918209 None:intergenic 40.0%
CTCAAAACTTGTGGTACTAG+AGG + chr6.4:49918323-49918342 MS.gene000298:CDS 40.0%
CTCAGTTGCATCTCTCAATA+GGG + chr6.4:49920083-49920102 MS.gene000298:CDS 40.0%
GAGAGTTGAAGTTCCAAGTT+TGG - chr6.4:49919968-49919987 None:intergenic 40.0%
GTACCAACACTTCTGATTGA+AGG - chr6.4:49920031-49920050 None:intergenic 40.0%
GTTGAAGTTCCAAGTTTGGA+TGG - chr6.4:49919964-49919983 None:intergenic 40.0%
TCACACTGTTCATTGACAGT+TGG + chr6.4:49918865-49918884 MS.gene000298:intron 40.0%
TCAGAAGATGGAAGTGAAGA+TGG - chr6.4:49917546-49917565 None:intergenic 40.0%
TCTCTCTTCAATAGCTTCCA+TGG - chr6.4:49918113-49918132 None:intergenic 40.0%
TCTTCTGCCATAACTACTGA+AGG + chr6.4:49920514-49920533 MS.gene000298:CDS 40.0%
TTCACCACCTTCAGTAGTTA+TGG - chr6.4:49920524-49920543 None:intergenic 40.0%
! ACTGGATTTTCCAAATGGTG+GGG - chr6.4:49919192-49919211 None:intergenic 40.0%
! ATTGTGGTCGTGTTTTCTCA+AGG + chr6.4:49918357-49918376 MS.gene000298:CDS 40.0%
! CACTGGATTTTCCAAATGGT+GGG - chr6.4:49919193-49919212 None:intergenic 40.0%
! CATGCTTTAGGTGATCTTGT+TGG + chr6.4:49918200-49918219 MS.gene000298:CDS 40.0%
! GATGCATAGAAGAAGGCATA+AGG + chr6.4:49918085-49918104 MS.gene000298:CDS 40.0%
! TTGCTATTGCTACTCTCCTT+TGG - chr6.4:49920169-49920188 None:intergenic 40.0%
!! AATCAGAAGTGTTGGTACTG+TGG + chr6.4:49920033-49920052 MS.gene000298:CDS 40.0%
!! ATCCTCTTTCTTTTTCGCAC+TGG + chr6.4:49917659-49917678 MS.gene000298:intron 40.0%
!! CAAACAACATGGCTTTGTTG+AGG + chr6.4:49920212-49920231 MS.gene000298:CDS 40.0%
!! TCCTCTTTCTTTTTCGCACT+GGG + chr6.4:49917660-49917679 MS.gene000298:intron 40.0%
!! TGGAAAAGGTATCTATCCAC+TGG - chr6.4:49919210-49919229 None:intergenic 40.0%
AAAAGGAAAAGAAGACCGGC+CGG + chr6.4:49917606-49917625 MS.gene000298:CDS 45.0%
AAGATGCTTGCAACATGGGA+AGG + chr6.4:49919705-49919724 MS.gene000298:CDS 45.0%
ACCCAGTGCGAAAAAGAAAG+AGG - chr6.4:49917664-49917683 None:intergenic 45.0%
AGAAGACAAACCTGGTGTTC+CGG - chr6.4:49917628-49917647 None:intergenic 45.0%
CCACCATTTGGAAAATCCAG+TGG + chr6.4:49919191-49919210 MS.gene000298:intron 45.0%
CTGCAACCAAGGATTCCAAA+GGG + chr6.4:49918049-49918068 MS.gene000298:CDS 45.0%
GAGGGTTCAAGAACAAGCAA+CGG + chr6.4:49920231-49920250 MS.gene000298:CDS 45.0%
GCATCAAGATGCTTGCAACA+TGG + chr6.4:49919700-49919719 MS.gene000298:CDS 45.0%
GGATCATGGTGCAAACAAGT+TGG - chr6.4:49918176-49918195 None:intergenic 45.0%
GTATGTGAGATCTGCAACCA+AGG + chr6.4:49918038-49918057 MS.gene000298:CDS 45.0%
GTTCTTGACAAGCATGCAGT+TGG - chr6.4:49919764-49919783 None:intergenic 45.0%
TACTGTGGAGGCTATTCAGA+AGG + chr6.4:49920048-49920067 MS.gene000298:CDS 45.0%
TCTGCAACCAAGGATTCCAA+AGG + chr6.4:49918048-49918067 MS.gene000298:CDS 45.0%
TCTGCCATAACTACTGAAGG+TGG + chr6.4:49920517-49920536 MS.gene000298:CDS 45.0%
! ACGCCTTCAATCAGAAGTGT+TGG + chr6.4:49920025-49920044 MS.gene000298:CDS 45.0%
! AGAGATGCAACTGAGTTGAC+CGG - chr6.4:49920078-49920097 None:intergenic 45.0%
! CATGCAGTTGGTGTTTGAAG+TGG - chr6.4:49919752-49919771 None:intergenic 45.0%
! CCACTGGATTTTCCAAATGG+TGG - chr6.4:49919194-49919213 None:intergenic 45.0%
! GAAGAAGGCATAAGGTACCA+TGG + chr6.4:49918093-49918112 MS.gene000298:CDS 45.0%
!! AGTCGTGTTGGTGTTGTTTG+TGG - chr6.4:49919993-49920012 None:intergenic 45.0%
!! CTTTCTTTTTCGCACTGGGT+CGG + chr6.4:49917664-49917683 MS.gene000298:intron 45.0%
!! GATTTTCCAAATGGTGGGGT+GGG - chr6.4:49919188-49919207 None:intergenic 45.0%
!! TGGTGCAAACAAGTTGGTTC+AGG - chr6.4:49918170-49918189 None:intergenic 45.0%
!! TTTCTTTTTCGCACTGGGTC+GGG + chr6.4:49917665-49917684 MS.gene000298:intron 45.0%
!! TTTGGTCCCTTTGGAATCCT+TGG - chr6.4:49918058-49918077 None:intergenic 45.0%
!!! ATACATTTTTTTTTATATTA+AGG - chr6.4:49918826-49918845 None:intergenic 5.0%
!!! ATATTTTTTAAAAAATGATA+TGG + chr6.4:49917843-49917862 MS.gene000298:intron 5.0%
!!! ATTTATATATATTTTTTGTA+GGG + chr6.4:49919661-49919680 MS.gene000298:intron 5.0%
AGAGACACCACTTCTGCATC+TGG - chr6.4:49917984-49918003 None:intergenic 50.0%
AGCAACGGAAAACGGAGCAT+TGG - chr6.4:49918903-49918922 None:intergenic 50.0%
GGAGGAGAAGAAGACAAACC+TGG - chr6.4:49917636-49917655 None:intergenic 50.0%
GTCTGTTGTCAGAGTCGTGT+TGG - chr6.4:49920005-49920024 None:intergenic 50.0%
!! CAGAAGTGTTGGTACTGTGG+AGG + chr6.4:49920036-49920055 MS.gene000298:CDS 50.0%
!! CGTGTTGGTGTTGTTTGTGG+TGG - chr6.4:49919990-49920009 None:intergenic 50.0%
!! GCTTGAAGCCTTGTTTGCCA+TGG - chr6.4:49919833-49919852 None:intergenic 50.0%
!! GGATTTTCCAAATGGTGGGG+TGG - chr6.4:49919189-49919208 None:intergenic 50.0%
CTTCTTCCCACCCCACCATT+TGG + chr6.4:49919179-49919198 MS.gene000298:intron 55.0%
! AATGGTGGGGTGGGAAGAAG+AGG - chr6.4:49919179-49919198 None:intergenic 55.0%
GACAAACCTGGTGTTCCGGC+CGG - chr6.4:49917624-49917643 None:intergenic 60.0%
AGAAGACCGGCCGGAACACC+AGG + chr6.4:49917615-49917634 MS.gene000298:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 49917522 49920582 49917522 ID=MS.gene000298
chr6.4 mRNA 49917522 49920582 49917522 ID=MS.gene000298.t1;Parent=MS.gene000298
chr6.4 exon 49917522 49917636 49917522 ID=MS.gene000298.t1.exon1;Parent=MS.gene000298.t1
chr6.4 CDS 49917522 49917636 49917522 ID=cds.MS.gene000298.t1;Parent=MS.gene000298.t1
chr6.4 exon 49917979 49918378 49917979 ID=MS.gene000298.t1.exon2;Parent=MS.gene000298.t1
chr6.4 CDS 49917979 49918378 49917979 ID=cds.MS.gene000298.t1;Parent=MS.gene000298.t1
chr6.4 exon 49919682 49920582 49919682 ID=MS.gene000298.t1.exon3;Parent=MS.gene000298.t1
chr6.4 CDS 49919682 49920582 49919682 ID=cds.MS.gene000298.t1;Parent=MS.gene000298.t1
Gene Sequence

>MS.gene000298

ATGTTAGCTAATAGCTTATCTCCATCTTCACTTCCATCTTCTGAGCCTTTTTCTTGCAATGAAAATGGCACTTCTTCCACTAATAAAAGGAAAAGAAGACCGGCCGGAACACCAGGTTTGTCTTCTTCTCCTCCTAAATCCTCTTTCTTTTTCGCACTGGGTCGGGTATCCTTCGTACTTCTAAACTCTAACCAGTGTTAGAGAAAATCTCAACCAATTCTTGAATCATGTTTTTTTTGTTGTATTGGATTCGACTATCGTTCTCAAGTCTATAAAAAAAACATTTTTTTTGACATGTTTTTTTCATTCTTTTGATTTATTATATTTTTTAAAAAATGATATGGTTGTTACTTATATGTTTATTTAATTCAATATGTATAGTTTTTTATAATTGTTTGATTTGATGCTAATTCTATAACTAAATTGTTATATTTTTATTTTGTTTGGTTTGGAATAGATCCAGATGCAGAAGTGGTGTCTCTCTCACCAAAAACATTGTTGGAATCAGATCGTTATGTATGTGAGATCTGCAACCAAGGATTCCAAAGGGACCAAAATTTACAGATGCATAGAAGAAGGCATAAGGTACCATGGAAGCTATTGAAGAGAGAAACACCAATTGTTAGAAAAAGAGTTTTTGTTTGTCCTGAACCAACTTGTTTGCACCATGATCCTTGTCATGCTTTAGGTGATCTTGTTGGTATCAAAAAACATTTTAGAAGAAAACATAGTAATCATAAACAATGGGTTTGTGAAAGATGTTCCAAAGGTTATGCAGTGCAATCTGATTATAAAGCTCATCTCAAAACTTGTGGTACTAGAGGTCATTCATGTGATTGTGGTCGTGTTTTCTCAAGGTAATTAATTAATTAAATTATTTATTTTTCTTTCATTCATAATTTCATATATCTAAATATATATAATTATACCTTTTTCAAGTATTTTTTTATAAATATATATTATCAACATTTATCCTATCATTGATTCTTGATTCATAAAATTGTTTTGAACAAATAGATCTAGCTATATATAGAATTTTTATTTTGAGAATCTTTATATATACTTCATGGGATGGGATATTGTAGTTTTTTTTCTTTTTAAATGAAACATTTTTAAACATTGTCTTATGATGATTATAATGAATTGAGGAAAACTTGAGTTGTTTTACTTGGGATTTGACTTTAAAAAAGGAAGATATGTTTTTTGGAAGGGAATATAAAAGTAAGGAAAATGGGAATGGAAAAACAAAAGGAGAGATAATTAAAATAAAAGTTTTTCAAAAATATATTTGACTGATGGAACCTTAATATAAAAAAAAATGTATTTTATTTTTCTTAACAAGATCACACTGTTCATTGACAGTTGGAGAACACAGTTTCCAATGCTCCGTTTTCCGTTGCTCTTATAACCGTTTTGTGTTTTCTTCAAAAATCATTCTTCCATTTCATTCTTTCTACACAAACAACAATACCTTAAACATTTCTTTATGTTTTTTTCATGTATATGTTTGTGTGAGAAACCATTAATATTATTTTTTTTTTTTTTTATAACATTGCAATTTAATTTCTTCTTAACAGTGAATCACATGAAAATGAGATTACTCATGAGAGTTCTTGACTATGTTTTGTCCTTTTTATTTTAGTGGAAACTGGAACCTCTTCTTCCCACCCCACCATTTGGAAAATCCAGTGGATAGATACCTTTTCCAACACCTTTTACATCCACTTTTTCATTTGTCTCTCTTTCTTTCTACCTTTCTTCCTTTGTACATTTTTTCCTAACTCTTTATATCTAATGAATTCCTTTTTATATTTTTGTTTCAAATCTCAATACTAGTAGCTTGTACTAAATTACTAATACTAACAAATGTTTCTTTTTCTCATCCTCTTCATCCATGTGTGTGATTTTATTATTATATATATAAAACACTATTATTAAATATTTATAGTCAATTATTATTGTTAATTAGGATTATGATGATGATGATGATAATAACACACATAGTATTTTGCAATTCAGCTTTATGTTTCTGGGTTTCCTCAATTCTTTAAATAGTAATATTTTATTGTTCAAAGATATTTGTATGAGTAAATTTACATATACTAGTAGGTTTTTTTTTTTCTCATCTTGTTTTCTTCCATTTATATATATTTTTTGTAGGGTTGAGAGCTTTATTGAGCATCAAGATGCTTGCAACATGGGAAGGATCCATAATTCAGAATCTCAACCACTTCAAACACCAACTGCATGCTTGTCAAGAACAGCTTCAAGTCCAAGTCCATCAAGTGAAACAAATTTCAATACATGTCCATGGCAAACAAGGCTTCAAGCAATGCCAAAAACAACAAAAGAATCAACAATATTCATGAACCCTATTACTCCTATTACTACTATTACACCTTCTTCTCAAACTTTCTCCAAGAACAATAAACTTCTACTCCATCCAAACTTGGAACTTCAACTCTCCACCACAAACAACACCAACACGACTCTGACAACAGACACGCCTTCAATCAGAAGTGTTGGTACTGTGGAGGCTATTCAGAAGGTTAACCGGTCAACTCAGTTGCATCTCTCAATAGGGTCTTCAGAAATGAGTCATGAGAAAAATGAATCAAACAATATTAGAAACTCAAACTCATCACCAAAGGAGAGTAGCAATAGCAATGAGAAAGTTCAATCAACAACAACAAACAACATGGCTTTGTTGAGGGTTCAAGAACAAGCAACGGAACAATTAAAGGTAGCTATGGCTGAAAAAGCTTATGCTGAAGAAGCAAGGAAACAAGCAAGGAAACAAATTGAAATGGCTGAACAAGAGTTCAACAATGCAAAGAGAATTAGACAACAAGCACAAAGTGAACTTGATAAAGCTTATGGATTGAAACAACATGCAATAAAGCAAATCAATTCAACTATGCTTCAAATCACTTGTCAAGCTTGCAAACAACAATTTCAACATGAAGATAACTCTTTGGTGTTGAGCTATGTTTCTTCTGCCATAACTACTGAAGGTGGTGAAGTAGAAAATGTTGATGGAAAAGGCAAAACAACTAATTAG
Protein sequence

>MS.gene000298.t1

MLANSLSPSSLPSSEPFSCNENGTSSTNKRKRRPAGTPDPDAEVVSLSPKTLLESDRYVCEICNQGFQRDQNLQMHRRRHKVPWKLLKRETPIVRKRVFVCPEPTCLHHDPCHALGDLVGIKKHFRRKHSNHKQWVCERCSKGYAVQSDYKAHLKTCGTRGHSCDCGRVFSRVESFIEHQDACNMGRIHNSESQPLQTPTACLSRTASSPSPSSETNFNTCPWQTRLQAMPKTTKESTIFMNPITPITTITPSSQTFSKNNKLLLHPNLELQLSTTNNTNTTLTTDTPSIRSVGTVEAIQKVNRSTQLHLSIGSSEMSHEKNESNNIRNSNSSPKESSNSNEKVQSTTTNNMALLRVQEQATEQLKVAMAEKAYAEEARKQARKQIEMAEQEFNNAKRIRQQAQSELDKAYGLKQHAIKQINSTMLQITCQACKQQFQHEDNSLVLSYVSSAITTEGGEVENVDGKGKTTN