AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000299

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000299.t1 MTR_1g023660 55.752 113 50 0 176 288 227 339 2.93e-45 156
MS.gene000299.t1 MTR_8g099780 32.540 252 163 4 40 288 64 311 5.94e-44 152
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000299.t1 AT2G01930 51.000 300 117 10 1 288 1 282 4.67e-94 279
MS.gene000299.t1 AT2G01930 51.000 300 117 10 1 288 1 282 4.67e-94 279
MS.gene000299.t1 AT1G14685 50.680 294 123 8 1 288 1 278 2.29e-87 262
MS.gene000299.t1 AT1G14685 50.680 294 123 8 1 288 1 278 2.29e-87 262
MS.gene000299.t1 AT1G14685 50.680 294 123 8 1 288 1 278 2.29e-87 262
MS.gene000299.t1 AT1G14685 50.680 294 123 8 1 288 1 278 2.29e-87 262
MS.gene000299.t1 AT1G68120 46.599 294 120 10 4 288 3 268 3.48e-76 233
MS.gene000299.t1 AT2G35550 54.795 146 64 2 143 288 82 225 1.10e-49 164
MS.gene000299.t1 AT2G35550 54.795 146 64 2 143 288 82 225 1.10e-49 164
MS.gene000299.t1 AT2G35550 54.795 146 64 2 143 288 82 225 1.10e-49 164
MS.gene000299.t1 AT2G35550 54.795 146 64 2 143 288 127 270 1.47e-49 165
MS.gene000299.t1 AT2G21240 32.576 264 163 5 28 288 44 295 1.24e-43 150
MS.gene000299.t1 AT2G21240 32.576 264 163 5 28 288 44 295 1.24e-43 150
MS.gene000299.t1 AT5G42520 56.604 106 45 1 184 288 176 281 1.04e-41 145
MS.gene000299.t1 AT5G42520 56.604 106 45 1 184 288 232 337 1.41e-41 146
MS.gene000299.t1 AT5G42520 56.604 106 45 1 184 288 236 341 1.84e-41 146
MS.gene000299.t1 AT4G38910 43.796 137 76 1 152 288 122 257 1.98e-41 144
MS.gene000299.t1 AT4G38910 43.165 139 78 1 150 288 145 282 2.87e-41 144

Find 97 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATATCACCATAGGGAAAT+TGG 0.193266 6.4:+49965799 MS.gene000299:CDS
CGATAGATTTGAGGACTTAT+TGG 0.234611 6.4:+49966448 MS.gene000299:CDS
TGCAATTAACGGGATTGATT+TGG 0.279573 6.4:+49966191 MS.gene000299:CDS
GATAGATTTGAGGACTTATT+GGG 0.312732 6.4:+49966449 MS.gene000299:CDS
CCAACAAGTTTGTTACTATC+AGG 0.343708 6.4:+49966484 MS.gene000299:CDS
ATTCCTGTTTGTTCTTGCAC+TGG 0.347046 6.4:+49966231 MS.gene000299:CDS
CACCTCAACAATGTTATAGA+TGG 0.355446 6.4:+49966256 MS.gene000299:CDS
ATGGACTTAACATAAGCAAT+TGG 0.360767 6.4:+49965653 MS.gene000299:CDS
CCGGAAAAGCCGCCTTTGAT+TGG 0.380482 6.4:+49965739 MS.gene000299:CDS
TTTCTAATCCGATAGATTTG+AGG 0.399273 6.4:+49966439 MS.gene000299:CDS
CTTACCCTATGGATTATATG+AGG 0.406292 6.4:+49965836 MS.gene000299:CDS
AATGATTCGACCATGTGAAT+TGG 0.408906 6.4:+49965975 MS.gene000299:CDS
CGAAATGCTGCGGTTATGTC+GGG 0.411374 6.4:+49965766 MS.gene000299:CDS
TGGACTTAACATAAGCAATT+GGG 0.420997 6.4:+49965654 MS.gene000299:CDS
CTACGGAAATTGCAATTAAC+GGG 0.422090 6.4:+49966181 MS.gene000299:CDS
TCATCATTTAAGAATCATCT+TGG 0.425330 6.4:+49965694 MS.gene000299:CDS
TTAGGAGACTTCGGCACCTT+CGG 0.426454 6.4:-49966072 None:intergenic
TCGACCATGTGAATTGGTTA+AGG 0.427145 6.4:+49965981 MS.gene000299:CDS
ACTACGGAAATTGCAATTAA+CGG 0.434305 6.4:+49966180 MS.gene000299:CDS
CATATCACCATAGGGAAATT+GGG 0.436973 6.4:+49965800 MS.gene000299:CDS
CCTGATAGTAACAAACTTGT+TGG 0.444146 6.4:-49966484 None:intergenic
CTATAACCTGGAGGGTTAGT+AGG 0.446214 6.4:-49965913 None:intergenic
TGATTCTTAAATGATGAAAC+AGG 0.446858 6.4:-49965688 None:intergenic
GCCTCAAACTACTTACCCTA+TGG 0.455211 6.4:+49965825 MS.gene000299:CDS
CTCTTCGGCTTCTTTGCCTT+AGG 0.455380 6.4:-49966090 None:intergenic
TCGAAATGCTGCGGTTATGT+CGG 0.458215 6.4:+49965765 MS.gene000299:CDS
TAATTTAGTGTTGTAATGGA+TGG 0.462352 6.4:+49965625 None:intergenic
ATAGATGGGGCTCGGGTGGA+TGG 0.464652 6.4:+49966271 MS.gene000299:CDS
TTCCTGTTTGTTCTTGCACT+GGG 0.468493 6.4:+49966232 MS.gene000299:CDS
GTTGTAATGGATGGAGATAA+TGG 0.478647 6.4:+49965634 None:intergenic
ACAATGTTATAGATGGGGCT+CGG 0.487220 6.4:+49966263 MS.gene000299:CDS
GGCACACGAGGTCCTCTCTT+CGG 0.493154 6.4:-49966105 None:intergenic
CCATTCACCTTCTCGACCAC+AGG 0.494618 6.4:-49966027 None:intergenic
TAGTATTCCCGAGACATCTT+CGG 0.495994 6.4:+49965939 MS.gene000299:CDS
ACAACTGATGCCATCGATGC+CGG 0.499881 6.4:+49965720 MS.gene000299:CDS
TTCTTTGCCTTAGGAGACTT+CGG 0.501514 6.4:-49966081 None:intergenic
GTCCCAGTGCAAGAACAAAC+AGG 0.501675 6.4:-49966234 None:intergenic
GTGCAAGAACAAACAGGAAT+CGG 0.503075 6.4:-49966228 None:intergenic
GAAGGAGAATTCTCGTCCTT+CGG 0.503811 6.4:-49966126 None:intergenic
CAATGTTATAGATGGGGCTC+GGG 0.507375 6.4:+49966264 MS.gene000299:CDS
CCTGTGGTCGAGAAGGTGAA+TGG 0.509475 6.4:+49966027 MS.gene000299:CDS
GAGGCATCCCAATTTCCCTA+TGG 0.509728 6.4:-49965807 None:intergenic
ATGATGAGCCGAAGATGTCT+CGG 0.513423 6.4:-49965947 None:intergenic
ACCTCAACAATGTTATAGAT+GGG 0.523017 6.4:+49966257 MS.gene000299:CDS
CCGCGTCGTTTAGTACTCAT+AGG 0.528654 6.4:-49966336 None:intergenic
CAATAAGTCCTCAAATCTAT+CGG 0.528692 6.4:-49966447 None:intergenic
GCATCCCTCATATAATCCAT+AGG 0.528771 6.4:-49965841 None:intergenic
GTCGAGAAGGTGAATGGAAC+TGG 0.530658 6.4:+49966033 MS.gene000299:CDS
GAAGGACGAGAATTCTCCTT+CGG 0.531353 6.4:+49966128 MS.gene000299:CDS
CTTGGTGTTCGTTGAACCGA+AGG 0.532361 6.4:-49966144 None:intergenic
TCCATAGGGTAAGTAGTTTG+AGG 0.540334 6.4:-49965826 None:intergenic
TGTTATAGATGGGGCTCGGG+TGG 0.542239 6.4:+49966267 MS.gene000299:CDS
ACCACAGGTGCTTCCTCCAT+CGG 0.542410 6.4:-49966012 None:intergenic
GCATTTCGACTTCCAATCAA+AGG 0.543040 6.4:-49965751 None:intergenic
CTAACGGTGCATATCACCAT+AGG 0.548956 6.4:+49965791 MS.gene000299:CDS
TTCTCGTCCTTCGGCACACG+AGG 0.552572 6.4:-49966117 None:intergenic
TGATGAGCCGAAGATGTCTC+GGG 0.558891 6.4:-49965946 None:intergenic
ATTAGAGAAACTTGCAGCCG+AGG 0.559521 6.4:+49966407 MS.gene000299:CDS
CATCCCTCATATAATCCATA+GGG 0.559663 6.4:-49965840 None:intergenic
ATTAGAAAAGTTATAACCCT+CGG 0.560267 6.4:-49966424 None:intergenic
AGGACTTATTGGGCAAAACA+TGG 0.561945 6.4:+49966459 MS.gene000299:CDS
ATGATACCTACTAACCCTCC+AGG 0.562585 6.4:+49965907 MS.gene000299:CDS
GCGGTTATGTCGGGAACTAA+CGG 0.570883 6.4:+49965775 MS.gene000299:CDS
GAGAGGACCTCGTGTGCCGA+AGG 0.571472 6.4:+49966110 MS.gene000299:CDS
GCCGGAAGGAAAATGAGTAT+AGG 0.573233 6.4:+49966369 MS.gene000299:CDS
TTAGAGAAACTTGCAGCCGA+GGG 0.574547 6.4:+49966408 MS.gene000299:CDS
TGGATTATATGAGGGATGCA+TGG 0.575577 6.4:+49965845 MS.gene000299:CDS
CGACGCGGTGCTAGAATAGC+CGG 0.576753 6.4:+49966351 MS.gene000299:CDS
GGAAGCACCTGTGGTCGAGA+AGG 0.588134 6.4:+49966020 MS.gene000299:CDS
GAAGGTGCCGAAGTCTCCTA+AGG 0.590939 6.4:+49966074 MS.gene000299:CDS
GCCGATGGAGGAAGCACCTG+TGG 0.594587 6.4:+49966011 MS.gene000299:CDS
TTACCCTATGGATTATATGA+GGG 0.595783 6.4:+49965837 MS.gene000299:CDS
GGACTTAACATAAGCAATTG+GGG 0.601047 6.4:+49965655 MS.gene000299:CDS
TTCGGTTCAACGAACACCAA+GGG 0.601561 6.4:+49966146 MS.gene000299:CDS
AAGGGCACAAAAGAAAACTA+CGG 0.609634 6.4:+49966164 MS.gene000299:CDS
CGTTTAGTACTCATAGGCAA+AGG 0.610952 6.4:-49966330 None:intergenic
ATGCATGGATTAGTAGTCAG+AGG 0.611474 6.4:+49965860 MS.gene000299:CDS
AAGGCAAAGAAGCCGAAGAG+AGG 0.622231 6.4:+49966093 MS.gene000299:CDS
CCCCATCTATAACATTGTTG+AGG 0.623582 6.4:-49966258 None:intergenic
TAAGAAGAGGCAAGGACCGA+AGG 0.629324 6.4:+49966056 MS.gene000299:CDS
TACTAGTACTATAACCTGGA+GGG 0.630649 6.4:-49965921 None:intergenic
GGAATACTAGTACTATAACC+TGG 0.630941 6.4:-49965925 None:intergenic
TCTTCCTTAACCAATTCACA+TGG 0.638004 6.4:-49965985 None:intergenic
TTTCGACTTCCAATCAAAGG+CGG 0.642330 6.4:-49965748 None:intergenic
TGCATGGATTAGTAGTCAGA+GGG 0.642777 6.4:+49965861 MS.gene000299:CDS
TGAATGGAACTGGTAAGAAG+AGG 0.650068 6.4:+49966043 MS.gene000299:CDS
GGAACTGGTAAGAAGAGGCA+AGG 0.651851 6.4:+49966048 MS.gene000299:CDS
TAACGGTGCATATCACCATA+GGG 0.655398 6.4:+49965792 MS.gene000299:CDS
TAAGGAAGAAATGCCGATGG+AGG 0.655765 6.4:+49965999 MS.gene000299:CDS
GATTGGAAGTCGAAATGCTG+CGG 0.656562 6.4:+49965756 MS.gene000299:CDS
CCTCAACAATGTTATAGATG+GGG 0.670226 6.4:+49966258 MS.gene000299:CDS
CTTCGGTTCAACGAACACCA+AGG 0.677515 6.4:+49966145 MS.gene000299:CDS
GGTTAAGGAAGAAATGCCGA+TGG 0.695305 6.4:+49965996 MS.gene000299:CDS
ATACTAGTACTATAACCTGG+AGG 0.700408 6.4:-49965922 None:intergenic
GCAGTCTGCATGCTGCACAA+CGG 0.723033 6.4:+49966293 MS.gene000299:CDS
GCGGTGCTAGAATAGCCGGA+AGG 0.737428 6.4:+49966355 MS.gene000299:CDS
CCTATGAGTACTAAACGACG+CGG 0.777544 6.4:+49966336 MS.gene000299:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ATTAGAAAAGTTATAACCCT+CGG - chr6.4:49966427-49966446 None:intergenic 25.0%
! TCATCATTTAAGAATCATCT+TGG + chr6.4:49965694-49965713 MS.gene000299:CDS 25.0%
! TGATTCTTAAATGATGAAAC+AGG - chr6.4:49965691-49965710 None:intergenic 25.0%
ACCTCAACAATGTTATAGAT+GGG + chr6.4:49966257-49966276 MS.gene000299:CDS 30.0%
ACTACGGAAATTGCAATTAA+CGG + chr6.4:49966180-49966199 MS.gene000299:CDS 30.0%
ATGGACTTAACATAAGCAAT+TGG + chr6.4:49965653-49965672 MS.gene000299:CDS 30.0%
CAATAAGTCCTCAAATCTAT+CGG - chr6.4:49966450-49966469 None:intergenic 30.0%
GATAGATTTGAGGACTTATT+GGG + chr6.4:49966449-49966468 MS.gene000299:CDS 30.0%
TGGACTTAACATAAGCAATT+GGG + chr6.4:49965654-49965673 MS.gene000299:CDS 30.0%
TTTCTAATCCGATAGATTTG+AGG + chr6.4:49966439-49966458 MS.gene000299:CDS 30.0%
! TTACCCTATGGATTATATGA+GGG + chr6.4:49965837-49965856 MS.gene000299:CDS 30.0%
AATGATTCGACCATGTGAAT+TGG + chr6.4:49965975-49965994 MS.gene000299:CDS 35.0%
ATACTAGTACTATAACCTGG+AGG - chr6.4:49965925-49965944 None:intergenic 35.0%
CACCTCAACAATGTTATAGA+TGG + chr6.4:49966256-49966275 MS.gene000299:CDS 35.0%
CATATCACCATAGGGAAATT+GGG + chr6.4:49965800-49965819 MS.gene000299:CDS 35.0%
CATCCCTCATATAATCCATA+GGG - chr6.4:49965843-49965862 None:intergenic 35.0%
CCTCAACAATGTTATAGATG+GGG + chr6.4:49966258-49966277 MS.gene000299:CDS 35.0%
CGATAGATTTGAGGACTTAT+TGG + chr6.4:49966448-49966467 MS.gene000299:CDS 35.0%
CTACGGAAATTGCAATTAAC+GGG + chr6.4:49966181-49966200 MS.gene000299:CDS 35.0%
GGAATACTAGTACTATAACC+TGG - chr6.4:49965928-49965947 None:intergenic 35.0%
GGACTTAACATAAGCAATTG+GGG + chr6.4:49965655-49965674 MS.gene000299:CDS 35.0%
TACTAGTACTATAACCTGGA+GGG - chr6.4:49965924-49965943 None:intergenic 35.0%
TCTTCCTTAACCAATTCACA+TGG - chr6.4:49965988-49966007 None:intergenic 35.0%
TGCAATTAACGGGATTGATT+TGG + chr6.4:49966191-49966210 MS.gene000299:CDS 35.0%
! AAGGGCACAAAAGAAAACTA+CGG + chr6.4:49966164-49966183 MS.gene000299:CDS 35.0%
! CTTACCCTATGGATTATATG+AGG + chr6.4:49965836-49965855 MS.gene000299:CDS 35.0%
! TCCTATACTCATTTTCCTTC+CGG - chr6.4:49966373-49966392 None:intergenic 35.0%
ACAATGTTATAGATGGGGCT+CGG + chr6.4:49966263-49966282 MS.gene000299:CDS 40.0%
AGGACTTATTGGGCAAAACA+TGG + chr6.4:49966459-49966478 MS.gene000299:CDS 40.0%
ATTCCTGTTTGTTCTTGCAC+TGG + chr6.4:49966231-49966250 MS.gene000299:CDS 40.0%
CCCCATCTATAACATTGTTG+AGG - chr6.4:49966261-49966280 None:intergenic 40.0%
CGTTTAGTACTCATAGGCAA+AGG - chr6.4:49966333-49966352 None:intergenic 40.0%
GCATATCACCATAGGGAAAT+TGG + chr6.4:49965799-49965818 MS.gene000299:CDS 40.0%
GCATCCCTCATATAATCCAT+AGG - chr6.4:49965844-49965863 None:intergenic 40.0%
GCATTTCGACTTCCAATCAA+AGG - chr6.4:49965754-49965773 None:intergenic 40.0%
GTGCAAGAACAAACAGGAAT+CGG - chr6.4:49966231-49966250 None:intergenic 40.0%
TAACGGTGCATATCACCATA+GGG + chr6.4:49965792-49965811 MS.gene000299:CDS 40.0%
TAGTATTCCCGAGACATCTT+CGG + chr6.4:49965939-49965958 MS.gene000299:CDS 40.0%
TCCATAGGGTAAGTAGTTTG+AGG - chr6.4:49965829-49965848 None:intergenic 40.0%
TCGACCATGTGAATTGGTTA+AGG + chr6.4:49965981-49966000 MS.gene000299:CDS 40.0%
TGAATGGAACTGGTAAGAAG+AGG + chr6.4:49966043-49966062 MS.gene000299:CDS 40.0%
TGGATTATATGAGGGATGCA+TGG + chr6.4:49965845-49965864 MS.gene000299:CDS 40.0%
TTCCTGTTTGTTCTTGCACT+GGG + chr6.4:49966232-49966251 MS.gene000299:CDS 40.0%
TTCTTTGCCTTAGGAGACTT+CGG - chr6.4:49966084-49966103 None:intergenic 40.0%
TTTCGACTTCCAATCAAAGG+CGG - chr6.4:49965751-49965770 None:intergenic 40.0%
! GTAGTTTTCTTTTGTGCCCT+TGG - chr6.4:49966165-49966184 None:intergenic 40.0%
!! ATGCATGGATTAGTAGTCAG+AGG + chr6.4:49965860-49965879 MS.gene000299:CDS 40.0%
!! TGCATGGATTAGTAGTCAGA+GGG + chr6.4:49965861-49965880 MS.gene000299:CDS 40.0%
ATGATACCTACTAACCCTCC+AGG + chr6.4:49965907-49965926 MS.gene000299:CDS 45.0%
ATGATGAGCCGAAGATGTCT+CGG - chr6.4:49965950-49965969 None:intergenic 45.0%
ATTAGAGAAACTTGCAGCCG+AGG + chr6.4:49966407-49966426 MS.gene000299:CDS 45.0%
CAATGTTATAGATGGGGCTC+GGG + chr6.4:49966264-49966283 MS.gene000299:CDS 45.0%
CCTATGAGTACTAAACGACG+CGG + chr6.4:49966336-49966355 MS.gene000299:CDS 45.0%
CTAACGGTGCATATCACCAT+AGG + chr6.4:49965791-49965810 MS.gene000299:CDS 45.0%
GAAGGACGAGAATTCTCCTT+CGG + chr6.4:49966128-49966147 MS.gene000299:CDS 45.0%
GAAGGAGAATTCTCGTCCTT+CGG - chr6.4:49966129-49966148 None:intergenic 45.0%
GATTGGAAGTCGAAATGCTG+CGG + chr6.4:49965756-49965775 MS.gene000299:CDS 45.0%
GCCGGAAGGAAAATGAGTAT+AGG + chr6.4:49966369-49966388 MS.gene000299:CDS 45.0%
GCCTCAAACTACTTACCCTA+TGG + chr6.4:49965825-49965844 MS.gene000299:CDS 45.0%
GGTTAAGGAAGAAATGCCGA+TGG + chr6.4:49965996-49966015 MS.gene000299:CDS 45.0%
TAAGGAAGAAATGCCGATGG+AGG + chr6.4:49965999-49966018 MS.gene000299:CDS 45.0%
TCGAAATGCTGCGGTTATGT+CGG + chr6.4:49965765-49965784 MS.gene000299:CDS 45.0%
TTAGAGAAACTTGCAGCCGA+GGG + chr6.4:49966408-49966427 MS.gene000299:CDS 45.0%
TTCGGTTCAACGAACACCAA+GGG + chr6.4:49966146-49966165 MS.gene000299:CDS 45.0%
! CTATAACCTGGAGGGTTAGT+AGG - chr6.4:49965916-49965935 None:intergenic 45.0%
AAGGCAAAGAAGCCGAAGAG+AGG + chr6.4:49966093-49966112 MS.gene000299:CDS 50.0%
ACAACTGATGCCATCGATGC+CGG + chr6.4:49965720-49965739 MS.gene000299:CDS 50.0%
CCGCGTCGTTTAGTACTCAT+AGG - chr6.4:49966339-49966358 None:intergenic 50.0%
CGAAATGCTGCGGTTATGTC+GGG + chr6.4:49965766-49965785 MS.gene000299:CDS 50.0%
CTCTTCGGCTTCTTTGCCTT+AGG - chr6.4:49966093-49966112 None:intergenic 50.0%
CTTCGGTTCAACGAACACCA+AGG + chr6.4:49966145-49966164 MS.gene000299:CDS 50.0%
GCGGTTATGTCGGGAACTAA+CGG + chr6.4:49965775-49965794 MS.gene000299:CDS 50.0%
GGAACTGGTAAGAAGAGGCA+AGG + chr6.4:49966048-49966067 MS.gene000299:CDS 50.0%
GTCCCAGTGCAAGAACAAAC+AGG - chr6.4:49966237-49966256 None:intergenic 50.0%
GTCGAGAAGGTGAATGGAAC+TGG + chr6.4:49966033-49966052 MS.gene000299:CDS 50.0%
TAAGAAGAGGCAAGGACCGA+AGG + chr6.4:49966056-49966075 MS.gene000299:CDS 50.0%
TGATGAGCCGAAGATGTCTC+GGG - chr6.4:49965949-49965968 None:intergenic 50.0%
TTAGGAGACTTCGGCACCTT+CGG - chr6.4:49966075-49966094 None:intergenic 50.0%
! CCAATCAAAGGCGGCTTTTC+CGG - chr6.4:49965742-49965761 None:intergenic 50.0%
! GAGGCATCCCAATTTCCCTA+TGG - chr6.4:49965810-49965829 None:intergenic 50.0%
!! CTTGGTGTTCGTTGAACCGA+AGG - chr6.4:49966147-49966166 None:intergenic 50.0%
ACCACAGGTGCTTCCTCCAT+CGG - chr6.4:49966015-49966034 None:intergenic 55.0%
CCATTCACCTTCTCGACCAC+AGG - chr6.4:49966030-49966049 None:intergenic 55.0%
CCGGAAAAGCCGCCTTTGAT+TGG + chr6.4:49965739-49965758 MS.gene000299:CDS 55.0%
CCTGTGGTCGAGAAGGTGAA+TGG + chr6.4:49966027-49966046 MS.gene000299:CDS 55.0%
GCAGTCTGCATGCTGCACAA+CGG + chr6.4:49966293-49966312 MS.gene000299:CDS 55.0%
TGTTATAGATGGGGCTCGGG+TGG + chr6.4:49966267-49966286 MS.gene000299:CDS 55.0%
!! GAAGGTGCCGAAGTCTCCTA+AGG + chr6.4:49966074-49966093 MS.gene000299:CDS 55.0%
ATAGATGGGGCTCGGGTGGA+TGG + chr6.4:49966271-49966290 MS.gene000299:CDS 60.0%
GGCACACGAGGTCCTCTCTT+CGG - chr6.4:49966108-49966127 None:intergenic 60.0%
TTCTCGTCCTTCGGCACACG+AGG - chr6.4:49966120-49966139 None:intergenic 60.0%
! CGACGCGGTGCTAGAATAGC+CGG + chr6.4:49966351-49966370 MS.gene000299:CDS 60.0%
! GCGGTGCTAGAATAGCCGGA+AGG + chr6.4:49966355-49966374 MS.gene000299:CDS 60.0%
!! GGAAGCACCTGTGGTCGAGA+AGG + chr6.4:49966020-49966039 MS.gene000299:CDS 60.0%
GAGAGGACCTCGTGTGCCGA+AGG + chr6.4:49966110-49966129 MS.gene000299:CDS 65.0%
!! GCCGATGGAGGAAGCACCTG+TGG + chr6.4:49966011-49966030 MS.gene000299:CDS 65.0%
!! GGCGGCTTTTCCGGCATCGA+TGG - chr6.4:49965733-49965752 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 49965640 49966505 49965640 ID=MS.gene000299
chr6.4 mRNA 49965640 49966505 49965640 ID=MS.gene000299.t1;Parent=MS.gene000299
chr6.4 exon 49965640 49966505 49965640 ID=MS.gene000299.t1.exon1;Parent=MS.gene000299.t1
chr6.4 CDS 49965640 49966505 49965640 ID=cds.MS.gene000299.t1;Parent=MS.gene000299.t1
Gene Sequence

>MS.gene000299

ATGGATGGAGATAATGGACTTAACATAAGCAATTGGGGATACTATGAACCTGTTTCATCATTTAAGAATCATCTTGGTTTACAACTGATGCCATCGATGCCGGAAAAGCCGCCTTTGATTGGAAGTCGAAATGCTGCGGTTATGTCGGGAACTAACGGTGCATATCACCATAGGGAAATTGGGATGCCTCAAACTACTTACCCTATGGATTATATGAGGGATGCATGGATTAGTAGTCAGAGGGATAATAAGTATATGAATATGAATATGATACCTACTAACCCTCCAGGTTATAGTACTAGTATTCCCGAGACATCTTCGGCTCATCATATTCAAATGATTCGACCATGTGAATTGGTTAAGGAAGAAATGCCGATGGAGGAAGCACCTGTGGTCGAGAAGGTGAATGGAACTGGTAAGAAGAGGCAAGGACCGAAGGTGCCGAAGTCTCCTAAGGCAAAGAAGCCGAAGAGAGGACCTCGTGTGCCGAAGGACGAGAATTCTCCTTCGGTTCAACGAACACCAAGGGCACAAAAGAAAACTACGGAAATTGCAATTAACGGGATTGATTTGGATATTTCAAGTATTCCGATTCCTGTTTGTTCTTGCACTGGGACACCTCAACAATGTTATAGATGGGGCTCGGGTGGATGGCAGTCTGCATGCTGCACAACGGCTATATCGATATATCCTTTGCCTATGAGTACTAAACGACGCGGTGCTAGAATAGCCGGAAGGAAAATGAGTATAGGAGCTTTTAAGAAAGTATTAGAGAAACTTGCAGCCGAGGGTTATAACTTTTCTAATCCGATAGATTTGAGGACTTATTGGGCAAAACATGGCACCAACAAGTTTGTTACTATCAG
Protein sequence

>MS.gene000299.t1

MDGDNGLNISNWGYYEPVSSFKNHLGLQLMPSMPEKPPLIGSRNAAVMSGTNGAYHHREIGMPQTTYPMDYMRDAWISSQRDNKYMNMNMIPTNPPGYSTSIPETSSAHHIQMIRPCELVKEEMPMEEAPVVEKVNGTGKKRQGPKVPKSPKAKKPKRGPRVPKDENSPSVQRTPRAQKKTTEIAINGIDLDISSIPIPVCSCTGTPQQCYRWGSGGWQSACCTTAISIYPLPMSTKRRGARIAGRKMSIGAFKKVLEKLAAEGYNFSNPIDLRTYWAKHGTNKFVTI