Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000439.t1 | XP_013447966.1 | 89.1 | 220 | 24 | 0 | 1 | 220 | 1 | 220 | 1.20E-98 | 369.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000439.t1 | Q39261 | 42.1 | 76 | 37 | 3 | 83 | 151 | 52 | 127 | 1.4e-07 | 58.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000439.t1 | A0A072TYR6 | 89.1 | 220 | 24 | 0 | 1 | 220 | 1 | 220 | 8.4e-99 | 369.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene000439.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000439.t1 | MTR_7g028890 | 89.091 | 220 | 24 | 0 | 1 | 220 | 1 | 220 | 1.44e-143 | 399 |
| MS.gene000439.t1 | MTR_7g022250 | 74.477 | 239 | 42 | 2 | 1 | 220 | 1 | 239 | 7.46e-126 | 355 |
| MS.gene000439.t1 | MTR_7g028915 | 71.368 | 234 | 31 | 2 | 1 | 220 | 1 | 212 | 8.21e-112 | 319 |
| MS.gene000439.t1 | MTR_7g022270 | 58.791 | 182 | 53 | 4 | 20 | 200 | 1 | 161 | 2.33e-69 | 209 |
| MS.gene000439.t1 | MTR_4g128340 | 30.714 | 140 | 78 | 4 | 83 | 220 | 47 | 169 | 1.20e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000439.t1 | AT5G57520 | 34.579 | 107 | 54 | 5 | 82 | 179 | 51 | 150 | 3.06e-11 | 59.7 |
Find 38 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTGTGGATATCAATATTA+TGG | 0.285744 | 7.1:+68678965 | MS.gene000439:CDS |
| AAGAAATGGAGAAATTTCTT+TGG | 0.293893 | 7.1:-68678759 | None:intergenic |
| CTTAAGGGTCAAATCAATAT+TGG | 0.328703 | 7.1:-68679215 | None:intergenic |
| CTCCTTCCTCTTGTTGCACA+TGG | 0.338528 | 7.1:-68678579 | None:intergenic |
| TATATATAAATCAAAGCTTA+AGG | 0.356106 | 7.1:-68679231 | None:intergenic |
| TGGTGCATTGCAACATATTC+CGG | 0.362580 | 7.1:+68679045 | MS.gene000439:CDS |
| CACCATCACTCCATTTCATA+TGG | 0.364608 | 7.1:+68678943 | MS.gene000439:CDS |
| AACAATATGATTCAACATGC+AGG | 0.449556 | 7.1:+68678992 | MS.gene000439:CDS |
| TATCCACACCCCATATGAAA+TGG | 0.452870 | 7.1:-68678953 | None:intergenic |
| AGTAGACAATTTGAAGATGA+TGG | 0.466141 | 7.1:+68678619 | MS.gene000439:CDS |
| TTCACTAGTCCACAGGCATT+AGG | 0.474635 | 7.1:+68678854 | MS.gene000439:CDS |
| TTGGATTCATTGTTGATCAC+AGG | 0.479894 | 7.1:+68678786 | MS.gene000439:CDS |
| TGAAGTTGGTAAAGTTTCAG+AGG | 0.484639 | 7.1:+68679132 | MS.gene000439:CDS |
| ACTATGTCAAAGATTGAAGT+TGG | 0.488225 | 7.1:+68679118 | MS.gene000439:CDS |
| ACCATCACTCCATTTCATAT+GGG | 0.489513 | 7.1:+68678944 | MS.gene000439:CDS |
| TGGTGTTTAGACTTATCCCT+TGG | 0.491881 | 7.1:-68678925 | None:intergenic |
| TGTTAGTGATGAAGTTGATC+AGG | 0.506770 | 7.1:+68679162 | MS.gene000439:CDS |
| CAAGCGAAAGGCAATTGTGC+TGG | 0.510108 | 7.1:+68678661 | MS.gene000439:CDS |
| GAAGTTGGTAAAGTTTCAGA+GGG | 0.513957 | 7.1:+68679133 | MS.gene000439:CDS |
| ATATATAAATCAAAGCTTAA+GGG | 0.520066 | 7.1:-68679230 | None:intergenic |
| ACTCCATTTCATATGGGGTG+TGG | 0.533278 | 7.1:+68678950 | MS.gene000439:CDS |
| TCATTCGTTTCATCTATCTC+CGG | 0.537068 | 7.1:-68679064 | None:intergenic |
| CAGAAAGTTCACTAGTCCAC+AGG | 0.575345 | 7.1:+68678847 | MS.gene000439:CDS |
| ACAATATGATTCAACATGCA+GGG | 0.581032 | 7.1:+68678993 | MS.gene000439:CDS |
| CTGATGCCATGTGCAACAAG+AGG | 0.598560 | 7.1:+68678573 | None:intergenic |
| TAGAATTCTGAGTTTCAACA+TGG | 0.602831 | 7.1:-68678691 | None:intergenic |
| TGCCATGTGCAACAAGAGGA+AGG | 0.602996 | 7.1:+68678577 | None:intergenic |
| GGAGATAGATGAAACGAATG+AGG | 0.622863 | 7.1:+68679066 | MS.gene000439:CDS |
| GTTGGTAAAGTTTCAGAGGG+AGG | 0.624522 | 7.1:+68679136 | MS.gene000439:CDS |
| CCATCACTCCATTTCATATG+GGG | 0.630153 | 7.1:+68678945 | MS.gene000439:CDS |
| GGAAGACAAAGAGACTCAAG+AGG | 0.634668 | 7.1:+68679183 | MS.gene000439:CDS |
| GTGCAACAAGAGGAAGGAGA+AGG | 0.635140 | 7.1:+68678583 | MS.gene000439:CDS |
| CCCCATATGAAATGGAGTGA+TGG | 0.640487 | 7.1:-68678945 | None:intergenic |
| GAACAATGAGAACAAGCGAA+AGG | 0.645636 | 7.1:+68678649 | MS.gene000439:CDS |
| ACTAGTCCACAGGCATTAGG+TGG | 0.661075 | 7.1:+68678857 | MS.gene000439:CDS |
| TGATGACCACCTAATGCCTG+TGG | 0.664402 | 7.1:-68678863 | None:intergenic |
| AGAATTCTGAGTTTCAACAT+GGG | 0.673130 | 7.1:-68678690 | None:intergenic |
| AGACAAAGAGACTCAAGAGG+AGG | 0.673262 | 7.1:+68679186 | MS.gene000439:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAAATGGAGAAATTTCTT+TGG | - | chr7.1:68678762-68678781 | None:intergenic | 25.0% |
| ! | ATGAATCCAAAAAGAAGAAA+TGG | - | chr7.1:68678776-68678795 | None:intergenic | 25.0% |
| !! | ATTTCTCCATTTCTTCTTTT+TGG | + | chr7.1:68678767-68678786 | MS.gene000439:CDS | 25.0% |
| !! | TTTTATGCAGGAAATTTGTA+TGG | + | chr7.1:68679025-68679044 | MS.gene000439:CDS | 25.0% |
| AACAATATGATTCAACATGC+AGG | + | chr7.1:68678992-68679011 | MS.gene000439:CDS | 30.0% | |
| ACAATATGATTCAACATGCA+GGG | + | chr7.1:68678993-68679012 | MS.gene000439:CDS | 30.0% | |
| ACTATGTCAAAGATTGAAGT+TGG | + | chr7.1:68679118-68679137 | MS.gene000439:CDS | 30.0% | |
| AGAATTCTGAGTTTCAACAT+GGG | - | chr7.1:68678693-68678712 | None:intergenic | 30.0% | |
| AGTAGACAATTTGAAGATGA+TGG | + | chr7.1:68678619-68678638 | MS.gene000439:CDS | 30.0% | |
| AGTCTCAATAAAAAGATCCA+AGG | + | chr7.1:68678908-68678927 | MS.gene000439:CDS | 30.0% | |
| CTTAAGGGTCAAATCAATAT+TGG | - | chr7.1:68679218-68679237 | None:intergenic | 30.0% | |
| GTCTCAATAAAAAGATCCAA+GGG | + | chr7.1:68678909-68678928 | MS.gene000439:CDS | 30.0% | |
| TAGAATTCTGAGTTTCAACA+TGG | - | chr7.1:68678694-68678713 | None:intergenic | 30.0% | |
| ACCATCACTCCATTTCATAT+GGG | + | chr7.1:68678944-68678963 | MS.gene000439:CDS | 35.0% | |
| GGGTGTGGATATCAATATTA+TGG | + | chr7.1:68678965-68678984 | MS.gene000439:CDS | 35.0% | |
| TCATTCGTTTCATCTATCTC+CGG | - | chr7.1:68679067-68679086 | None:intergenic | 35.0% | |
| TTGGATTCATTGTTGATCAC+AGG | + | chr7.1:68678786-68678805 | MS.gene000439:CDS | 35.0% | |
| ! | CTTCTTCAGCAACATCTTTT+TGG | - | chr7.1:68678724-68678743 | None:intergenic | 35.0% |
| ! | TGTTAGTGATGAAGTTGATC+AGG | + | chr7.1:68679162-68679181 | MS.gene000439:CDS | 35.0% |
| !! | GAAGTTGGTAAAGTTTCAGA+GGG | + | chr7.1:68679133-68679152 | MS.gene000439:CDS | 35.0% |
| !! | TGAAGTTGGTAAAGTTTCAG+AGG | + | chr7.1:68679132-68679151 | MS.gene000439:CDS | 35.0% |
| CACCATCACTCCATTTCATA+TGG | + | chr7.1:68678943-68678962 | MS.gene000439:CDS | 40.0% | |
| CCATCACTCCATTTCATATG+GGG | + | chr7.1:68678945-68678964 | MS.gene000439:CDS | 40.0% | |
| GAACAATGAGAACAAGCGAA+AGG | + | chr7.1:68678649-68678668 | MS.gene000439:CDS | 40.0% | |
| GGAGATAGATGAAACGAATG+AGG | + | chr7.1:68679066-68679085 | MS.gene000439:CDS | 40.0% | |
| TATCCACACCCCATATGAAA+TGG | - | chr7.1:68678956-68678975 | None:intergenic | 40.0% | |
| TGGTGCATTGCAACATATTC+CGG | + | chr7.1:68679045-68679064 | MS.gene000439:CDS | 40.0% | |
| TGGTGTTTAGACTTATCCCT+TGG | - | chr7.1:68678928-68678947 | None:intergenic | 40.0% | |
| ! | GGGTCATCAAACTTTTATGC+AGG | + | chr7.1:68679013-68679032 | MS.gene000439:CDS | 40.0% |
| ACTCCATTTCATATGGGGTG+TGG | + | chr7.1:68678950-68678969 | MS.gene000439:CDS | 45.0% | |
| AGACAAAGAGACTCAAGAGG+AGG | + | chr7.1:68679186-68679205 | MS.gene000439:CDS | 45.0% | |
| CAGAAAGTTCACTAGTCCAC+AGG | + | chr7.1:68678847-68678866 | MS.gene000439:CDS | 45.0% | |
| CCCCATATGAAATGGAGTGA+TGG | - | chr7.1:68678948-68678967 | None:intergenic | 45.0% | |
| GGAAGACAAAGAGACTCAAG+AGG | + | chr7.1:68679183-68679202 | MS.gene000439:CDS | 45.0% | |
| TTCACTAGTCCACAGGCATT+AGG | + | chr7.1:68678854-68678873 | MS.gene000439:CDS | 45.0% | |
| ! | GTTGGTAAAGTTTCAGAGGG+AGG | + | chr7.1:68679136-68679155 | MS.gene000439:CDS | 45.0% |
| ACTAGTCCACAGGCATTAGG+TGG | + | chr7.1:68678857-68678876 | MS.gene000439:CDS | 50.0% | |
| CAAGCGAAAGGCAATTGTGC+TGG | + | chr7.1:68678661-68678680 | MS.gene000439:CDS | 50.0% | |
| GTGCAACAAGAGGAAGGAGA+AGG | + | chr7.1:68678583-68678602 | MS.gene000439:CDS | 50.0% | |
| TGATGACCACCTAATGCCTG+TGG | - | chr7.1:68678866-68678885 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 68678581 | 68679243 | 68678581 | ID=MS.gene000439 |
| chr7.1 | mRNA | 68678581 | 68679243 | 68678581 | ID=MS.gene000439.t1;Parent=MS.gene000439 |
| chr7.1 | exon | 68678581 | 68679243 | 68678581 | ID=MS.gene000439.t1.exon1;Parent=MS.gene000439.t1 |
| chr7.1 | CDS | 68678581 | 68679243 | 68678581 | ID=cds.MS.gene000439.t1;Parent=MS.gene000439.t1 |
| Gene Sequence |
| Protein sequence |
