AlfalfaGEDB | MS.gene000446

M. sativa cultivar XinJiangDaYe / MS.gene000446

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005575 GO:0005634 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0097159 GO:0110165 GO:1901363
PFAM: PF03110.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000446.t1	XP_013447951.1	95.9	368	12	3	1	366	1	367	4.60E-201	710.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000446.t1	Q49I57	43.2	227	99	6	75	279	105	323	6.2e-37	156.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000446.t1	A0A072TYQ4	95.9	368	12	3	1	366	1	367	3.3e-201	710.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene000446.t1	TF	SBP

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000446.t1	MTR_7g028740	95.924	368	12	3	1	366	1	367	0.0	725
MS.gene000446.t1	MTR_3g099080	34.925	398	205	12	1	366	1	376	2.54e-58	194
MS.gene000446.t1	MTR_8g096780	48.259	201	96	6	17	211	36	234	6.56e-50	172
MS.gene000446.t1	MTR_4g109770	39.713	209	106	6	25	223	75	273	1.31e-36	138
MS.gene000446.t1	MTR_2g461920	51.538	130	61	1	70	199	166	293	3.03e-34	132
MS.gene000446.t1	MTR_3g085180	58.242	91	35	1	67	154	154	244	9.74e-33	127
MS.gene000446.t1	MTR_7g092930	63.636	88	32	0	70	157	63	150	1.55e-32	124
MS.gene000446.t1	MTR_5g046670	56.000	100	44	0	74	173	243	342	5.01e-32	126
MS.gene000446.t1	MTR_8g463140	64.557	79	28	0	75	153	65	143	1.48e-31	117
MS.gene000446.t1	MTR_4g088555	58.427	89	35	1	62	148	48	136	3.88e-31	115

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000446.t1	AT5G50670	45.833	192	88	5	24	200	41	231	3.09e-43	154
MS.gene000446.t1	AT5G50670	45.833	192	88	5	24	200	41	231	3.09e-43	154
MS.gene000446.t1	AT5G50570	45.833	192	88	5	24	200	41	231	3.09e-43	154
MS.gene000446.t1	AT5G50570	45.833	192	88	5	24	200	41	231	3.09e-43	154
MS.gene000446.t1	AT1G69170	66.292	89	29	1	62	149	110	198	2.71e-33	128
MS.gene000446.t1	AT1G76580	43.506	154	86	1	2	154	40	193	7.64e-32	127
MS.gene000446.t1	AT1G76580	43.506	154	86	1	2	154	40	193	7.81e-32	127
MS.gene000446.t1	AT2G42200	63.218	87	32	0	75	161	74	160	9.01e-32	123
MS.gene000446.t1	AT1G76580	43.506	154	86	1	2	154	40	193	9.72e-32	127
MS.gene000446.t1	AT5G43270	46.903	113	60	0	75	187	169	281	2.11e-31	123

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Find 86 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACAAATACTGGACCATT+CGG	0.495656	7.1:+68936188	None:intergenic
AAAACTCCTGTTGTGTTGGT+TGG	0.434108	7.1:-68938361	MS.gene000446:CDS
AAAAGAGCGCGTTTGCAAAA+TGG	0.355601	7.1:-68938481	MS.gene000446:CDS
AACCAACACAACAGGAGTTT+TGG	0.287936	7.1:+68938363	None:intergenic
AAGGAGATTCAATGGATTTG+AGG	0.446707	7.1:-68938603	MS.gene000446:CDS
AAGGCGAAGGATAAGCCGAA+TGG	0.688289	7.1:-68936203	MS.gene000446:CDS
AAGTTCATGTACAATTACAA+AGG	0.619439	7.1:-68937783	MS.gene000446:intron
AATATGATTTGCTCGGTTGA+TGG	0.434096	7.1:-68938451	MS.gene000446:CDS
ACAACCCGAGTCAAAAGATC+CGG	0.476548	7.1:+68936364	None:intergenic
ACCAGATTCAGCTTCAGTTA+TGG	0.414958	7.1:+68936586	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACAAATACTGGACCATT+CGG	+	chr7.1:68938561-68938580	None:intergenic	30.0%
AATGAGCTGAAAACAACTTA+CGG	-	chr7.1:68936681-68936700	MS.gene000446:intron	30.0%
AATTTGATTAGAGAGAGGAT+TGG	+	chr7.1:68937612-68937631	None:intergenic	30.0%
AGATGATTCTACTATTGAAG+AGG	-	chr7.1:68937351-68937370	MS.gene000446:intron	30.0%
AGTAACTGTTGGATTTAGAT+CGG	-	chr7.1:68937767-68937786	MS.gene000446:intron	30.0%
ATTAGTGCTTTAGTAACTGT+TGG	-	chr7.1:68937756-68937775	MS.gene000446:intron	30.0%
GAAAAATGAAGGAGATTCAA+TGG	-	chr7.1:68936135-68936154	MS.gene000446:CDS	30.0%
GAATTGTAGTTGTTTGTTTC+GGG	-	chr7.1:68936450-68936469	MS.gene000446:CDS	30.0%
GACAAACATAAACAGGATAA+GGG	-	chr7.1:68938213-68938232	MS.gene000446:intron	30.0%
GATGATTCTACTATTGAAGA+GGG	-	chr7.1:68937352-68937371	MS.gene000446:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	68936082	68938686	68936082	ID=MS.gene000446
chr7.1	mRNA	68936082	68938686	68936082	ID=MS.gene000446.t1;Parent=MS.gene000446
chr7.1	exon	68938325	68938686	68938325	ID=MS.gene000446.t1.exon1;Parent=MS.gene000446.t1
chr7.1	CDS	68938325	68938686	68938325	ID=cds.MS.gene000446.t1;Parent=MS.gene000446.t1
chr7.1	exon	68937784	68937920	68937784	ID=MS.gene000446.t1.exon2;Parent=MS.gene000446.t1
chr7.1	CDS	68937784	68937920	68937784	ID=cds.MS.gene000446.t1;Parent=MS.gene000446.t1
chr7.1	exon	68936082	68936683	68936082	ID=MS.gene000446.t1.exon3;Parent=MS.gene000446.t1
chr7.1	CDS	68936082	68936683	68936082	ID=cds.MS.gene000446.t1;Parent=MS.gene000446.t1

Gene Sequence

>MS.gene000446

ATGGATTGGGATTGGAAAGAGTTTACTTGGGATCCATCTGGATTGGAGGAGTTGAAAAATGAAGGAGATTCAATGGATTTGAGGCTTGGTGATCAGGCTTCTGATTCATTGGAGAAATCAGTTCATGATTCAGGAAAGGAAGATTCAAAAGCTGTGTCGTCGTCGTCACCTACTAGATCGTTGAAAAGAGCGCGTTTGCAAAATGGATCGCAGAATATGATTTGCTCGGTTGATGGATGTAACTCTGATCTTCGTGATTGTAGAGAGTATCATAAGCGTCATAGGGTGTGCGAAAAGCATTCCAAAACTCCTGTTGTGTTGGTTGGAGGTAAGCAGCAGAGGTTCTGCCAACAATGCAGCAGGTATATGAATTGTAGTTGTTTGTTTCGGGTTAATAACTTGTTGAATTTTGTTGTTTTAGTGTCATGTTTGAATAAACAGCTTAATTAAACGCTTATAGCATTTTTGTTTATCATTACAAGTGTTTATAATTTATGTATTTATGTGTACGTTATTTCTATAACAGTATAAAATAAAGTCAAACTGTTTTCATATAAGCTAGAAGTTGATTTCATAAGCTATTCTGCAAAGAATATGAAAATGAGCTGAAAACAACTTACGGACATGTCATAAGCTGTTTCCATATAGAAGCTATCTGAATATTCTAAAAATGGCTTATTTGAATAGATAAGCTTAAAAATAACAATCCAAACACATCATGTTTTGAGGTTGTTTTGTAACATGGGGTGATGTCAATTTGATGTAGGTTTCATTCACTTGCGGAGTTTGATGAGGTTAAGAGGAGTTGCAGGAAACGTCTTGATGGACATAATAAGCGCAGAAGGAAACCTCAACCACAGTCTCTTTTCATGGCTGCTGAGAAGTTCATGTACAATTACAAAGGTTTGTGTCTTTTGCATCTCATGATTCTAAGATAGATGGCTTATTTCTGTCGATGTTTAACAAGATCTCAGTTTTATGATTGTCAAACCTGAGATGAAAGAGGGAATTATGACGAAGATATAACGTTTTTTATGGAAGAAGATACGCATGATGAATAAGTAAGAAATCACATATACATAACAATCCATAAGGTTTTGACAATAGAATTTAGAGTATCTCTCAAAATTTGTTTCAAAATTTTATTTACCATGAACTGAATTCTGAGCTAAGCTTAATAATTAGCTATAGTTACTTAGCAACCTTTGGATATACAACTTAATTAAAAACTTACTTTAGAAGTGCTTATTGTGAAAGAGTTTATGTTTAAGATGATTCTACTATTGAAGAGGGAATGCATAAAACTCTGCGTAAAGCTAAAATTAGCTTGTGCAATAAGTTTCTAGGAGATGCTTATGCAAATATGTTAAAAGCAACTTAACAAACATGTCGTAATCTATTTTCATTTGCTTTTCCAAATAATCTATGTCATAAGATAAGTTTAAATAAGCGCAAGTGTTTATGTCATAAGATAAGTTTAAATAAGCGCAAGTGTTTATGTCATAAGATAAGTTTAAATAAGCTCTTCCAATCCTCTCTCTAATCAAATTTAATGACAAAGAAAGTGACCTTCCGTCTTTATTTTCACAAGAAAGATGGTGGAGAAATATGAAACTTTGCTAATATTTCCCCATGGATTTCAATTGCAAAAAATGGATGCCCTGCATTTACACGATTAGTGCTTTAGTAACTGTTGGATTTAGATCGGACATTTCACAAAATTTGTTGATTTAATCTTTTCGAAATTCAAAATAGTGAGCTGGAAATTCACACTGTCCAATCTTGATCCAACAGTCACTGATGTGTTGACTGCGTGAATTCATAGTATTCCAACTGCAGGGAATCTTGATCCTTGATTTTCGGCATGCTCAAACTGAACTAATATGATTACTGCTGGCATCTGTTTTTTTTTTTTTTAGTCATGATTTATTACATTATTTTTGTCTTCCACAAGCACAAGTAATATGGAGTTTTAATGCTGTTTTATCTTTGTACAATTTTAGGACCTAGAATCCTGCACTTTGGTAGCCCTCAGACATATGTGAATCCTATTATGAGAAACATTTGGCCTGCAACTTCCATAACTGAAGCTGAATCTGGTTATGATCATCAAAGACTTTTGTATCGAATCGACAAACATAAACAGGATAAGGGACATCCTCATTGGCAAGAAATTCTCCCTAAGTCCGGCGATGTTAACAAAGCAGCACCTGGAACTCCTATCAGCCATCCTATCCGCGGTGCCGTTGGTTCATCCGCAGGTGAAAAAGGTGGTAGGAAGCTCTCTTCTGATGGCAAGCCCGGATCTTTTGACTCGGGTTGTGCTCTCTATCTTCTGTCAACACTTCAAACACAAAGCTCAGAGTTGAGTTTGATGCAGTCAAGCATCAACAACAGTCCAACACAATCTTCGTCTGGAACGTTGCATTTTGATGCTACGAAGGAGTGCTCGTACTCCGGAAAGGCGAAGGATAAGCCGAATGGTCCAGTATTTGTTTTAGATGGAAACACGACAAACCTTCATTGCAATGGGATGCTGCAAATGGGTCCTAATGGATTGATCGAAAATGATAACTCAGTGACTCTTCCATTCTTCTGGGAGTAG

Protein sequence

>MS.gene000446.t1

MDWDWKEFTWDPSGLEELKNEGDSMDLRLGDQASDSLEKSVHDSGKEDSKAVSSSSPTRSLKRARLQNGSQNMICSVDGCNSDLRDCREYHKRHRVCEKHSKTPVVLVGGKQQRFCQQCSRFHSLAEFDEVKRSCRKRLDGHNKRRRKPQPQSLFMAAEKFMYNYKGPRILHFGSPQTYVNPIMRNIWPATSITEAESGYDHQRLLYRIDKHKQDKGHPHWQEILPKSGDVNKAAPGTPISHPIRGAVGSSAGEKGGRKLSSDGKPGSFDSGCALYLLSTLQTQSSELSLMQSSINNSPTQSSSGTLHFDATKECSYSGKAKDKPNGPVFVLDGNTTNLHCNGMLQMGPNGLIENDNSVTLPFFWE