AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000446

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000446.t1 MTR_7g028740 95.924 368 12 3 1 366 1 367 0.0 725
MS.gene000446.t1 MTR_3g099080 34.925 398 205 12 1 366 1 376 2.54e-58 194
MS.gene000446.t1 MTR_8g096780 48.259 201 96 6 17 211 36 234 6.56e-50 172
MS.gene000446.t1 MTR_4g109770 39.713 209 106 6 25 223 75 273 1.31e-36 138
MS.gene000446.t1 MTR_2g461920 51.538 130 61 1 70 199 166 293 3.03e-34 132
MS.gene000446.t1 MTR_3g085180 58.242 91 35 1 67 154 154 244 9.74e-33 127
MS.gene000446.t1 MTR_7g092930 63.636 88 32 0 70 157 63 150 1.55e-32 124
MS.gene000446.t1 MTR_5g046670 56.000 100 44 0 74 173 243 342 5.01e-32 126
MS.gene000446.t1 MTR_8g463140 64.557 79 28 0 75 153 65 143 1.48e-31 117
MS.gene000446.t1 MTR_4g088555 58.427 89 35 1 62 148 48 136 3.88e-31 115
MS.gene000446.t1 MTR_1g053715 61.446 83 32 0 75 157 74 156 2.41e-30 119
MS.gene000446.t1 MTR_2g014200 60.714 84 33 0 69 152 58 141 3.46e-30 113
MS.gene000446.t1 MTR_7g444860 63.750 80 29 0 75 154 149 228 3.74e-30 117
MS.gene000446.t1 MTR_1g035010 41.558 154 74 4 2 154 45 183 4.90e-30 122
MS.gene000446.t1 MTR_2g046550 43.571 140 64 2 17 156 96 220 1.08e-29 121
MS.gene000446.t1 MTR_2g078770 66.667 75 25 0 74 148 57 131 4.05e-29 111
MS.gene000446.t1 MTR_7g110320 59.551 89 34 1 62 148 130 218 3.89e-28 116
MS.gene000446.t1 MTR_1g086250 55.670 97 42 1 59 154 130 226 3.90e-28 116
MS.gene000446.t1 MTR_7g110320 59.551 89 34 1 62 148 130 218 4.83e-28 116
MS.gene000446.t1 MTR_8g005960 56.122 98 38 2 58 150 165 262 7.76e-28 111
MS.gene000446.t1 MTR_8g080690 59.494 79 32 0 75 153 151 229 9.70e-28 112
MS.gene000446.t1 MTR_8g080680 52.500 80 38 0 75 154 132 211 1.50e-23 100
MS.gene000446.t1 MTR_8g080670 55.405 74 33 0 74 147 161 234 3.64e-23 100
MS.gene000446.t1 MTR_2g020620 52.542 59 28 0 90 148 105 163 7.12e-16 79.7
MS.gene000446.t1 MTR_8g066240 43.158 95 50 2 25 115 8 102 4.88e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000446.t1 AT5G50670 45.833 192 88 5 24 200 41 231 3.09e-43 154
MS.gene000446.t1 AT5G50670 45.833 192 88 5 24 200 41 231 3.09e-43 154
MS.gene000446.t1 AT5G50570 45.833 192 88 5 24 200 41 231 3.09e-43 154
MS.gene000446.t1 AT5G50570 45.833 192 88 5 24 200 41 231 3.09e-43 154
MS.gene000446.t1 AT1G69170 66.292 89 29 1 62 149 110 198 2.71e-33 128
MS.gene000446.t1 AT1G76580 43.506 154 86 1 2 154 40 193 7.64e-32 127
MS.gene000446.t1 AT1G76580 43.506 154 86 1 2 154 40 193 7.81e-32 127
MS.gene000446.t1 AT2G42200 63.218 87 32 0 75 161 74 160 9.01e-32 123
MS.gene000446.t1 AT1G76580 43.506 154 86 1 2 154 40 193 9.72e-32 127
MS.gene000446.t1 AT5G43270 46.903 113 60 0 75 187 169 281 2.11e-31 123
MS.gene000446.t1 AT5G43270 46.903 113 60 0 75 187 169 281 2.11e-31 123
MS.gene000446.t1 AT5G43270 46.903 113 60 0 75 187 169 281 2.11e-31 123
MS.gene000446.t1 AT1G20980 43.558 163 71 4 2 154 49 200 8.25e-31 124
MS.gene000446.t1 AT3G60030 58.333 96 38 1 61 154 111 206 1.10e-30 124
MS.gene000446.t1 AT1G27370 55.140 107 44 2 48 150 145 251 2.29e-30 119
MS.gene000446.t1 AT1G27370 55.140 107 44 2 48 150 145 251 3.69e-30 119
MS.gene000446.t1 AT1G27370 55.140 107 44 2 48 150 145 251 3.69e-30 119
MS.gene000446.t1 AT1G27370 55.140 107 44 2 48 150 145 251 3.69e-30 119
MS.gene000446.t1 AT1G27370 55.140 107 44 2 48 150 145 251 3.69e-30 119
MS.gene000446.t1 AT1G27370 55.140 107 44 2 48 150 145 251 3.69e-30 119
MS.gene000446.t1 AT1G27370 55.140 107 44 2 48 150 145 251 3.69e-30 119
MS.gene000446.t1 AT2G33810 61.333 75 29 0 74 148 53 127 7.62e-30 112
MS.gene000446.t1 AT1G53160 52.688 93 44 0 63 155 42 134 1.31e-29 112
MS.gene000446.t1 AT1G53160 52.688 93 44 0 63 155 42 134 2.00e-29 112
MS.gene000446.t1 AT1G53160 52.688 93 44 0 63 155 42 134 2.00e-29 112
MS.gene000446.t1 AT1G27360 65.789 76 26 0 75 150 175 250 3.78e-29 116
MS.gene000446.t1 AT1G27360 65.789 76 26 0 75 150 175 250 3.78e-29 116
MS.gene000446.t1 AT1G27360 65.789 76 26 0 75 150 175 250 3.78e-29 116
MS.gene000446.t1 AT1G27360 65.789 76 26 0 75 150 175 250 3.78e-29 116
MS.gene000446.t1 AT2G47070 63.291 79 29 0 74 152 105 183 2.14e-28 117
MS.gene000446.t1 AT3G57920 50.980 102 45 2 75 176 59 155 9.77e-28 112
MS.gene000446.t1 AT3G15270 61.333 75 29 0 74 148 62 136 1.17e-27 107
MS.gene000446.t1 AT1G02065 63.514 74 27 0 75 148 188 261 4.39e-27 110
MS.gene000446.t1 AT5G18830 42.424 99 57 0 62 160 125 223 2.68e-20 93.2
MS.gene000446.t1 AT5G18830 42.424 99 57 0 62 160 125 223 2.86e-20 92.8
MS.gene000446.t1 AT5G18830 42.424 99 57 0 62 160 125 223 3.02e-20 92.8
MS.gene000446.t1 AT2G47070 71.698 53 15 0 100 152 2 54 4.30e-17 83.2
MS.gene000446.t1 AT1G02065 44.304 79 37 2 58 129 166 244 1.57e-14 73.2

Find 86 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCTTATCCTTCGCCTTTC+CGG 0.134360 7.1:+68936208 None:intergenic
CAGTGACTCTTCCATTCTTC+TGG 0.209211 7.1:-68936088 MS.gene000446:CDS
GAGAAATCAGTTCATGATTC+AGG 0.275617 7.1:-68938553 MS.gene000446:CDS
AACCAACACAACAGGAGTTT+TGG 0.287936 7.1:+68938363 None:intergenic
CTACCAAAGTGCAGGATTCT+AGG 0.315434 7.1:+68936659 None:intergenic
GGCCAAATGTTTCTCATAAT+AGG 0.321028 7.1:+68936617 None:intergenic
CTCTCTTCTGATGGCAAGCC+CGG 0.326414 7.1:-68936383 MS.gene000446:CDS
GGATTTGAGGCTTGGTGATC+AGG 0.330157 7.1:-68938590 MS.gene000446:CDS
ATGAGGTTAAGAGGAGTTGC+AGG 0.333125 7.1:-68937875 MS.gene000446:CDS
GGTTCATCCGCAGGTGAAAA+AGG 0.348606 7.1:-68936416 MS.gene000446:CDS
AAAAGAGCGCGTTTGCAAAA+TGG 0.355601 7.1:-68938481 MS.gene000446:CDS
TTTCCATCTAAAACAAATAC+TGG 0.355846 7.1:+68936179 None:intergenic
TCATATACCTGCTGCATTGT+TGG 0.364223 7.1:+68938317 None:intergenic
GAGAGTATCATAAGCGTCAT+AGG 0.369267 7.1:-68938402 MS.gene000446:CDS
GCTTCAGTTATGGAAGTTGC+AGG 0.374630 7.1:+68936596 None:intergenic
ACGAAGGAGTGCTCGTACTC+CGG 0.377570 7.1:-68936227 MS.gene000446:CDS
CGACAAACATAAACAGGATA+AGG 0.380487 7.1:-68936534 MS.gene000446:CDS
GGGATTGGAAAGAGTTTACT+TGG 0.383641 7.1:-68938657 MS.gene000446:CDS
TGATCAGGCTTCTGATTCAT+TGG 0.384148 7.1:-68938575 MS.gene000446:CDS
TCCATAACTGAAGCTGAATC+TGG 0.398324 7.1:-68936587 MS.gene000446:CDS
GCAGCATCCCATTGCAATGA+AGG 0.401478 7.1:+68936147 None:intergenic
TTGTTAACATCGCCGGACTT+AGG 0.408591 7.1:+68936485 None:intergenic
ACCAGATTCAGCTTCAGTTA+TGG 0.414958 7.1:+68936586 None:intergenic
TCGAATCGACAAACATAAAC+AGG 0.415548 7.1:-68936540 MS.gene000446:CDS
TGTTAACATCGCCGGACTTA+GGG 0.417871 7.1:+68936486 None:intergenic
AGTGACTCTTCCATTCTTCT+GGG 0.418501 7.1:-68936087 MS.gene000446:CDS
CGGAGTTTGATGAGGTTAAG+AGG 0.427340 7.1:-68937884 MS.gene000446:CDS
GAGTTTACTTGGGATCCATC+TGG 0.428398 7.1:-68938646 MS.gene000446:CDS
AATATGATTTGCTCGGTTGA+TGG 0.434096 7.1:-68938451 MS.gene000446:CDS
AAAACTCCTGTTGTGTTGGT+TGG 0.434108 7.1:-68938361 MS.gene000446:CDS
GGATGGCTGATAGGAGTTCC+AGG 0.442493 7.1:+68936455 None:intergenic
GATTCAATGGATTTGAGGCT+TGG 0.443840 7.1:-68938598 MS.gene000446:CDS
AAGGAGATTCAATGGATTTG+AGG 0.446707 7.1:-68938603 MS.gene000446:CDS
ATGCTGCAAATGGGTCCTAA+TGG 0.447508 7.1:-68936131 MS.gene000446:CDS
CGACAAACCTTCATTGCAAT+GGG 0.449586 7.1:-68936154 MS.gene000446:CDS
ATCCTATTATGAGAAACATT+TGG 0.453663 7.1:-68936619 MS.gene000446:CDS
TTGCAATGGGATGCTGCAAA+TGG 0.467365 7.1:-68936141 MS.gene000446:CDS
ATGAAAAGAGACTGTGGTTG+AGG 0.473034 7.1:+68937816 None:intergenic
ACAACCCGAGTCAAAAGATC+CGG 0.476548 7.1:+68936364 None:intergenic
TACTTGGGATCCATCTGGAT+TGG 0.477477 7.1:-68938641 MS.gene000446:CDS
GACAAACATAAACAGGATAA+GGG 0.477832 7.1:-68936533 MS.gene000446:CDS
TGCTGCTTTGTTAACATCGC+CGG 0.478377 7.1:+68936478 None:intergenic
TTGGGATCCATCTGGATTGG+AGG 0.483016 7.1:-68938638 MS.gene000446:CDS
CATCCTATCCGCGGTGCCGT+TGG 0.484865 7.1:-68936437 MS.gene000446:CDS
AGTTGCAGGAAACGTCTTGA+TGG 0.488371 7.1:-68937861 MS.gene000446:CDS
AAAACAAATACTGGACCATT+CGG 0.495656 7.1:+68936188 None:intergenic
AGGATAAGGGACATCCTCAT+TGG 0.499189 7.1:-68936520 MS.gene000446:CDS
CCGCGGATAGGATGGCTGAT+AGG 0.503089 7.1:+68936446 None:intergenic
AGACGCTACTCCCAGAAGAA+TGG 0.507419 7.1:+68936077 None:intergenic
CTCGTACTCCGGAAAGGCGA+AGG 0.509356 7.1:-68936216 MS.gene000446:CDS
TTACCTCCAACCAACACAAC+AGG 0.509843 7.1:+68938355 None:intergenic
CAACGGCACCGCGGATAGGA+TGG 0.519767 7.1:+68936438 None:intergenic
ACGACAAACCTTCATTGCAA+TGG 0.528803 7.1:-68936155 MS.gene000446:CDS
AGTCCAACACAATCTTCGTC+TGG 0.529463 7.1:-68936269 MS.gene000446:CDS
ACTCCTGTTGTGTTGGTTGG+AGG 0.530507 7.1:-68938358 MS.gene000446:CDS
CAAGAAATTCTCCCTAAGTC+CGG 0.531212 7.1:-68936497 MS.gene000446:CDS
TTCACCTGCGGATGAACCAA+CGG 0.533214 7.1:+68936421 None:intergenic
TTCAACTCCTCCAATCCAGA+TGG 0.535266 7.1:+68938631 None:intergenic
GGGAGAATTTCTTGCCAATG+AGG 0.536926 7.1:+68936506 None:intergenic
GAACCAACGGCACCGCGGAT+AGG 0.537355 7.1:+68936434 None:intergenic
GGTGCCGTTGGTTCATCCGC+AGG 0.539865 7.1:-68936425 MS.gene000446:CDS
GGACCTAGAATCCTGCACTT+TGG 0.540161 7.1:-68936662 MS.gene000446:CDS
TTCCAAAACTCCTGTTGTGT+TGG 0.540226 7.1:-68938365 MS.gene000446:CDS
TGCAATGGGATGCTGCAAAT+GGG 0.554526 7.1:-68936140 MS.gene000446:CDS
GGTTCTGCCAACAATGCAGC+AGG 0.557731 7.1:-68938324 MS.gene000446:intron
TCTGAGGGCTACCAAAGTGC+AGG 0.563556 7.1:+68936651 None:intergenic
ATGTAGGTTTCATTCACTTG+CGG 0.564106 7.1:-68937904 MS.gene000446:intron
GATGTTAACAAAGCAGCACC+TGG 0.566098 7.1:-68936473 MS.gene000446:CDS
ATCGCAGAATATGATTTGCT+CGG 0.580677 7.1:-68938458 MS.gene000446:CDS
GGTAGGAAGCTCTCTTCTGA+TGG 0.581427 7.1:-68936392 MS.gene000446:CDS
ATCAGTTCATGATTCAGGAA+AGG 0.585059 7.1:-68938548 MS.gene000446:CDS
ATGGACATAATAAGCGCAGA+AGG 0.585971 7.1:-68937842 MS.gene000446:CDS
AGAGTATCATAAGCGTCATA+GGG 0.590533 7.1:-68938401 MS.gene000446:CDS
TTCACTTGCGGAGTTTGATG+AGG 0.591231 7.1:-68937892 MS.gene000446:CDS
TGGTTGGAGGTAAGCAGCAG+AGG 0.595809 7.1:-68938345 MS.gene000446:CDS
ATAGGATTCACATATGTCTG+AGG 0.596972 7.1:+68936635 None:intergenic
TAGGATTCACATATGTCTGA+GGG 0.610035 7.1:+68936636 None:intergenic
CAACCCGAGTCAAAAGATCC+GGG 0.610877 7.1:+68936365 None:intergenic
GGATTGGAAAGAGTTTACTT+GGG 0.616910 7.1:-68938656 MS.gene000446:CDS
GTTCCAGACGAAGATTGTGT+TGG 0.619281 7.1:+68936266 None:intergenic
AAGTTCATGTACAATTACAA+AGG 0.619439 7.1:-68937783 MS.gene000446:intron
GGAGTGCTCGTACTCCGGAA+AGG 0.631193 7.1:-68936222 MS.gene000446:CDS
CCTATCAGCCATCCTATCCG+CGG 0.675251 7.1:-68936446 MS.gene000446:CDS
AAGGCGAAGGATAAGCCGAA+TGG 0.688289 7.1:-68936203 MS.gene000446:CDS
GCAGCCATGAAAAGAGACTG+TGG 0.701662 7.1:+68937810 None:intergenic
CGGATGAACCAACGGCACCG+CGG 0.749670 7.1:+68936429 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATCTTTGTACAATTTT+AGG - chr7.1:68938063-68938082 MS.gene000446:intron 15.0%
!! ATCTTATGACATAGATTATT+TGG + chr7.1:68937499-68937518 None:intergenic 20.0%
!!! CTATCTGAATATTCTAAAAA+TGG - chr7.1:68936733-68936752 MS.gene000446:intron 20.0%
!!! TAATTAAGTTGTATATCCAA+AGG + chr7.1:68937287-68937306 None:intergenic 20.0%
! AAGTTCATGTACAATTACAA+AGG - chr7.1:68936963-68936982 MS.gene000446:intron 25.0%
! ATATTCAGATAGCTTCTATA+TGG + chr7.1:68936725-68936744 None:intergenic 25.0%
! ATCCTATTATGAGAAACATT+TGG - chr7.1:68938127-68938146 MS.gene000446:intron 25.0%
! ATTCTATTGTCAAAACCTTA+TGG + chr7.1:68937172-68937191 None:intergenic 25.0%
! CATATACATAACAATCCATA+AGG - chr7.1:68937154-68937173 MS.gene000446:intron 25.0%
! CATTAAATTTGATTAGAGAG+AGG + chr7.1:68937617-68937636 None:intergenic 25.0%
! CTTTCTTGTGAAAATAAAGA+CGG + chr7.1:68937657-68937676 None:intergenic 25.0%
! TGAATTGTAGTTGTTTGTTT+CGG - chr7.1:68936449-68936468 MS.gene000446:CDS 25.0%
! TGGATTTCAATTGCAAAAAA+TGG - chr7.1:68937715-68937734 MS.gene000446:intron 25.0%
!! TTTCCATCTAAAACAAATAC+TGG + chr7.1:68938570-68938589 None:intergenic 25.0%
!! TTTTTGCAATTGAAATCCAT+GGG + chr7.1:68937714-68937733 None:intergenic 25.0%
!!! CGAAGATATAACGTTTTTTA+TGG - chr7.1:68937097-68937116 MS.gene000446:intron 25.0%
!!! CTTTATTTTCACAAGAAAGA+TGG - chr7.1:68937658-68937677 MS.gene000446:intron 25.0%
!!! TTTTTTGCAATTGAAATCCA+TGG + chr7.1:68937715-68937734 None:intergenic 25.0%
AAAACAAATACTGGACCATT+CGG + chr7.1:68938561-68938580 None:intergenic 30.0%
AATGAGCTGAAAACAACTTA+CGG - chr7.1:68936681-68936700 MS.gene000446:intron 30.0%
AATTTGATTAGAGAGAGGAT+TGG + chr7.1:68937612-68937631 None:intergenic 30.0%
AGATGATTCTACTATTGAAG+AGG - chr7.1:68937351-68937370 MS.gene000446:intron 30.0%
AGTAACTGTTGGATTTAGAT+CGG - chr7.1:68937767-68937786 MS.gene000446:intron 30.0%
ATTAGTGCTTTAGTAACTGT+TGG - chr7.1:68937756-68937775 MS.gene000446:intron 30.0%
GAAAAATGAAGGAGATTCAA+TGG - chr7.1:68936135-68936154 MS.gene000446:CDS 30.0%
GAATTGTAGTTGTTTGTTTC+GGG - chr7.1:68936450-68936469 MS.gene000446:CDS 30.0%
GACAAACATAAACAGGATAA+GGG - chr7.1:68938213-68938232 MS.gene000446:intron 30.0%
GATGATTCTACTATTGAAGA+GGG - chr7.1:68937352-68937371 MS.gene000446:intron 30.0%
TATAGTTACTTAGCAACCTT+TGG - chr7.1:68937268-68937287 MS.gene000446:intron 30.0%
TGAACTAATATGATTACTGC+TGG - chr7.1:68937956-68937975 MS.gene000446:intron 30.0%
! GAATCTTGATCCTTGATTTT+CGG - chr7.1:68937922-68937941 MS.gene000446:intron 30.0%
! TATTTTCACAAGAAAGATGG+TGG - chr7.1:68937661-68937680 MS.gene000446:intron 30.0%
! TCATTTTCGATCAATCCATT+AGG + chr7.1:68938633-68938652 None:intergenic 30.0%
! TTTTGCAATTGAAATCCATG+GGG + chr7.1:68937713-68937732 None:intergenic 30.0%
!! TCTCATGATTCTAAGATAGA+TGG - chr7.1:68937001-68937020 MS.gene000446:intron 30.0%
!! TTGAGGTTGTTTTGTAACAT+GGG - chr7.1:68936805-68936824 MS.gene000446:intron 30.0%
!! TTTGAGGTTGTTTTGTAACA+TGG - chr7.1:68936804-68936823 MS.gene000446:intron 30.0%
ACCTCAAAACATGATGTGTT+TGG + chr7.1:68936792-68936811 None:intergenic 35.0%
ACTTTGCTAATATTTCCCCA+TGG - chr7.1:68937695-68937714 MS.gene000446:intron 35.0%
AGCTTGTGCAATAAGTTTCT+AGG - chr7.1:68937406-68937425 MS.gene000446:intron 35.0%
ATAATTCCCTCTTTCATCTC+AGG + chr7.1:68937075-68937094 None:intergenic 35.0%
ATAGGATTCACATATGTCTG+AGG + chr7.1:68938114-68938133 None:intergenic 35.0%
ATCAGTTCATGATTCAGGAA+AGG - chr7.1:68936198-68936217 MS.gene000446:CDS 35.0%
ATGTAGGTTTCATTCACTTG+CGG - chr7.1:68936842-68936861 MS.gene000446:intron 35.0%
ATTCATAGTATTCCAACTGC+AGG - chr7.1:68937899-68937918 MS.gene000446:CDS 35.0%
CGAAATTCAAAATAGTGAGC+TGG - chr7.1:68937822-68937841 MS.gene000446:CDS 35.0%
CGACAAACATAAACAGGATA+AGG - chr7.1:68938212-68938231 MS.gene000446:intron 35.0%
CTTGTGAAAATAAAGACGGA+AGG + chr7.1:68937653-68937672 None:intergenic 35.0%
GAGAAATCAGTTCATGATTC+AGG - chr7.1:68936193-68936212 MS.gene000446:CDS 35.0%
GGATTGGAAAGAGTTTACTT+GGG - chr7.1:68936090-68936109 MS.gene000446:CDS 35.0%
GGCCAAATGTTTCTCATAAT+AGG + chr7.1:68938132-68938151 None:intergenic 35.0%
TAGGATTCACATATGTCTGA+GGG + chr7.1:68938113-68938132 None:intergenic 35.0%
TCGAATCGACAAACATAAAC+AGG - chr7.1:68938206-68938225 MS.gene000446:intron 35.0%
TTAGCTCAGAATTCAGTTCA+TGG + chr7.1:68937234-68937253 None:intergenic 35.0%
TTCATAGTATTCCAACTGCA+GGG - chr7.1:68937900-68937919 MS.gene000446:CDS 35.0%
TTCCACAAGCACAAGTAATA+TGG - chr7.1:68938027-68938046 MS.gene000446:intron 35.0%
TTGGAGGAGTTGAAAAATGA+AGG - chr7.1:68936124-68936143 MS.gene000446:CDS 35.0%
! AAGGAGATTCAATGGATTTG+AGG - chr7.1:68936143-68936162 MS.gene000446:CDS 35.0%
! AGAGTATCATAAGCGTCATA+GGG - chr7.1:68936345-68936364 MS.gene000446:CDS 35.0%
! TCCAAACACATCATGTTTTG+AGG - chr7.1:68936788-68936807 MS.gene000446:intron 35.0%
!! AATATGATTTGCTCGGTTGA+TGG - chr7.1:68936295-68936314 MS.gene000446:CDS 35.0%
!! ATCGCAGAATATGATTTGCT+CGG - chr7.1:68936288-68936307 MS.gene000446:CDS 35.0%
!! TGAGGTTGTTTTGTAACATG+GGG - chr7.1:68936806-68936825 MS.gene000446:intron 35.0%
!!! GGTCCAGTATTTGTTTTAGA+TGG - chr7.1:68938564-68938583 MS.gene000446:CDS 35.0%
ACCAGATTCAGCTTCAGTTA+TGG + chr7.1:68938163-68938182 None:intergenic 40.0%
ACGACAAACCTTCATTGCAA+TGG - chr7.1:68938591-68938610 MS.gene000446:CDS 40.0%
AGCACTAATCGTGTAAATGC+AGG + chr7.1:68937745-68937764 None:intergenic 40.0%
AGTGACTCTTCCATTCTTCT+GGG - chr7.1:68938659-68938678 MS.gene000446:CDS 40.0%
AGTGACTGTTGGATCAAGAT+TGG + chr7.1:68937861-68937880 None:intergenic 40.0%
ATGAAAAGAGACTGTGGTTG+AGG + chr7.1:68936933-68936952 None:intergenic 40.0%
ATGGACATAATAAGCGCAGA+AGG - chr7.1:68936904-68936923 MS.gene000446:intron 40.0%
CAAGAAATTCTCCCTAAGTC+CGG - chr7.1:68938249-68938268 MS.gene000446:intron 40.0%
CGACAAACCTTCATTGCAAT+GGG - chr7.1:68938592-68938611 MS.gene000446:CDS 40.0%
CTCCATATTACTTGTGCTTG+TGG + chr7.1:68938032-68938051 None:intergenic 40.0%
GCACTAATCGTGTAAATGCA+GGG + chr7.1:68937744-68937763 None:intergenic 40.0%
GGGATTGGAAAGAGTTTACT+TGG - chr7.1:68936089-68936108 MS.gene000446:CDS 40.0%
GGGTGATGTCAATTTGATGT+AGG - chr7.1:68936826-68936845 MS.gene000446:intron 40.0%
GTCAAACCTGAGATGAAAGA+GGG - chr7.1:68937066-68937085 MS.gene000446:intron 40.0%
TCATATACCTGCTGCATTGT+TGG + chr7.1:68936432-68936451 None:intergenic 40.0%
TCCATAACTGAAGCTGAATC+TGG - chr7.1:68938159-68938178 MS.gene000446:intron 40.0%
TGATCAGGCTTCTGATTCAT+TGG - chr7.1:68936171-68936190 MS.gene000446:CDS 40.0%
TGTCAAACCTGAGATGAAAG+AGG - chr7.1:68937065-68937084 MS.gene000446:intron 40.0%
TTCCAAAACTCCTGTTGTGT+TGG - chr7.1:68936381-68936400 MS.gene000446:CDS 40.0%
TTTGAGCATGCCGAAAATCA+AGG + chr7.1:68937935-68937954 None:intergenic 40.0%
! AAAACTCCTGTTGTGTTGGT+TGG - chr7.1:68936385-68936404 MS.gene000446:CDS 40.0%
! AAAAGAGCGCGTTTGCAAAA+TGG - chr7.1:68936265-68936284 MS.gene000446:CDS 40.0%
! AACCAACACAACAGGAGTTT+TGG + chr7.1:68936386-68936405 None:intergenic 40.0%
! GATTCAATGGATTTGAGGCT+TGG - chr7.1:68936148-68936167 MS.gene000446:CDS 40.0%
! GCTCTTTTCAACGATCTAGT+AGG + chr7.1:68936253-68936272 None:intergenic 40.0%
! TCAACCACAGTCTCTTTTCA+TGG - chr7.1:68936932-68936951 MS.gene000446:intron 40.0%
!! GAGAGTATCATAAGCGTCAT+AGG - chr7.1:68936344-68936363 MS.gene000446:CDS 40.0%
!! GTTGCATTTTGATGCTACGA+AGG - chr7.1:68938503-68938522 MS.gene000446:CDS 40.0%
ACAACCCGAGTCAAAAGATC+CGG + chr7.1:68938385-68938404 None:intergenic 45.0%
AGGATAAGGGACATCCTCAT+TGG - chr7.1:68938226-68938245 MS.gene000446:intron 45.0%
AGTCCAACACAATCTTCGTC+TGG - chr7.1:68938477-68938496 MS.gene000446:CDS 45.0%
AGTTGCAGGAAACGTCTTGA+TGG - chr7.1:68936885-68936904 MS.gene000446:intron 45.0%
ATGAGGTTAAGAGGAGTTGC+AGG - chr7.1:68936871-68936890 MS.gene000446:intron 45.0%
ATGCTGCAAATGGGTCCTAA+TGG - chr7.1:68938615-68938634 MS.gene000446:CDS 45.0%
CAGTGACTCTTCCATTCTTC+TGG - chr7.1:68938658-68938677 MS.gene000446:CDS 45.0%
CGGAGTTTGATGAGGTTAAG+AGG - chr7.1:68936862-68936881 MS.gene000446:intron 45.0%
CTACCAAAGTGCAGGATTCT+AGG + chr7.1:68938090-68938109 None:intergenic 45.0%
GAGTTTACTTGGGATCCATC+TGG - chr7.1:68936100-68936119 MS.gene000446:CDS 45.0%
GATGTTAACAAAGCAGCACC+TGG - chr7.1:68938273-68938292 MS.gene000446:intron 45.0%
GCTTCAGTTATGGAAGTTGC+AGG + chr7.1:68938153-68938172 None:intergenic 45.0%
GGGAGAATTTCTTGCCAATG+AGG + chr7.1:68938243-68938262 None:intergenic 45.0%
GTCAACACATCAGTGACTGT+TGG + chr7.1:68937872-68937891 None:intergenic 45.0%
GTTCCAGACGAAGATTGTGT+TGG + chr7.1:68938483-68938502 None:intergenic 45.0%
TACTTGGGATCCATCTGGAT+TGG - chr7.1:68936105-68936124 MS.gene000446:CDS 45.0%
TGCAATGGGATGCTGCAAAT+GGG - chr7.1:68938606-68938625 MS.gene000446:CDS 45.0%
TGTTAACATCGCCGGACTTA+GGG + chr7.1:68938263-68938282 None:intergenic 45.0%
TTACCTCCAACCAACACAAC+AGG + chr7.1:68936394-68936413 None:intergenic 45.0%
TTCAACTCCTCCAATCCAGA+TGG + chr7.1:68936118-68936137 None:intergenic 45.0%
TTCACTTGCGGAGTTTGATG+AGG - chr7.1:68936854-68936873 MS.gene000446:intron 45.0%
TTGCAATGGGATGCTGCAAA+TGG - chr7.1:68938605-68938624 MS.gene000446:CDS 45.0%
TTGTTAACATCGCCGGACTT+AGG + chr7.1:68938264-68938283 None:intergenic 45.0%
!! TGCTGCTTTGTTAACATCGC+CGG + chr7.1:68938271-68938290 None:intergenic 45.0%
AAGGCGAAGGATAAGCCGAA+TGG - chr7.1:68938543-68938562 MS.gene000446:CDS 50.0%
CAACCCGAGTCAAAAGATCC+GGG + chr7.1:68938384-68938403 None:intergenic 50.0%
GCAGCATCCCATTGCAATGA+AGG + chr7.1:68938602-68938621 None:intergenic 50.0%
GCAGCCATGAAAAGAGACTG+TGG + chr7.1:68936939-68936958 None:intergenic 50.0%
GGACCTAGAATCCTGCACTT+TGG - chr7.1:68938084-68938103 MS.gene000446:intron 50.0%
GGATCAAGATTCCCTGCAGT+TGG + chr7.1:68937914-68937933 None:intergenic 50.0%
GGTAGGAAGCTCTCTTCTGA+TGG - chr7.1:68938354-68938373 MS.gene000446:CDS 50.0%
GGTTCATCCGCAGGTGAAAA+AGG - chr7.1:68938330-68938349 MS.gene000446:CDS 50.0%
TCATCCGCAGGTGAAAAAGG+TGG - chr7.1:68938333-68938352 MS.gene000446:CDS 50.0%
TTCACCTGCGGATGAACCAA+CGG + chr7.1:68938328-68938347 None:intergenic 50.0%
! TTGGGATCCATCTGGATTGG+AGG - chr7.1:68936108-68936127 MS.gene000446:CDS 50.0%
!! AAGCCCGGATCTTTTGACTC+GGG - chr7.1:68938378-68938397 MS.gene000446:CDS 50.0%
!! ACTCCTGTTGTGTTGGTTGG+AGG - chr7.1:68936388-68936407 MS.gene000446:CDS 50.0%
!! CAAGCCCGGATCTTTTGACT+CGG - chr7.1:68938377-68938396 MS.gene000446:CDS 50.0%
!! CCTACCACCTTTTTCACCTG+CGG + chr7.1:68938340-68938359 None:intergenic 50.0%
!! GGATTTGAGGCTTGGTGATC+AGG - chr7.1:68936156-68936175 MS.gene000446:CDS 50.0%
CCGCAGGTGAAAAAGGTGGT+AGG - chr7.1:68938337-68938356 MS.gene000446:CDS 55.0%
CCTATCAGCCATCCTATCCG+CGG - chr7.1:68938300-68938319 MS.gene000446:intron 55.0%
CGGCTTATCCTTCGCCTTTC+CGG + chr7.1:68938541-68938560 None:intergenic 55.0%
CTCTCTTCTGATGGCAAGCC+CGG - chr7.1:68938363-68938382 MS.gene000446:CDS 55.0%
GGATGGCTGATAGGAGTTCC+AGG + chr7.1:68938294-68938313 None:intergenic 55.0%
GGTTCTGCCAACAATGCAGC+AGG - chr7.1:68936422-68936441 MS.gene000446:CDS 55.0%
TCTGAGGGCTACCAAAGTGC+AGG + chr7.1:68938098-68938117 None:intergenic 55.0%
TGGTTGGAGGTAAGCAGCAG+AGG - chr7.1:68936401-68936420 MS.gene000446:CDS 55.0%
!! ACGAAGGAGTGCTCGTACTC+CGG - chr7.1:68938519-68938538 MS.gene000446:CDS 55.0%
CCGCGGATAGGATGGCTGAT+AGG + chr7.1:68938303-68938322 None:intergenic 60.0%
CTCGTACTCCGGAAAGGCGA+AGG - chr7.1:68938530-68938549 MS.gene000446:CDS 60.0%
! GGAGTGCTCGTACTCCGGAA+AGG - chr7.1:68938524-68938543 MS.gene000446:CDS 60.0%
CAACGGCACCGCGGATAGGA+TGG + chr7.1:68938311-68938330 None:intergenic 65.0%
CGGATGAACCAACGGCACCG+CGG + chr7.1:68938320-68938339 None:intergenic 65.0%
GAACCAACGGCACCGCGGAT+AGG + chr7.1:68938315-68938334 None:intergenic 65.0%
!! CATCCTATCCGCGGTGCCGT+TGG - chr7.1:68938309-68938328 MS.gene000446:intron 65.0%
!! GGTGCCGTTGGTTCATCCGC+AGG - chr7.1:68938321-68938340 MS.gene000446:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 68936082 68938686 68936082 ID=MS.gene000446
chr7.1 mRNA 68936082 68938686 68936082 ID=MS.gene000446.t1;Parent=MS.gene000446
chr7.1 exon 68938325 68938686 68938325 ID=MS.gene000446.t1.exon1;Parent=MS.gene000446.t1
chr7.1 CDS 68938325 68938686 68938325 ID=cds.MS.gene000446.t1;Parent=MS.gene000446.t1
chr7.1 exon 68937784 68937920 68937784 ID=MS.gene000446.t1.exon2;Parent=MS.gene000446.t1
chr7.1 CDS 68937784 68937920 68937784 ID=cds.MS.gene000446.t1;Parent=MS.gene000446.t1
chr7.1 exon 68936082 68936683 68936082 ID=MS.gene000446.t1.exon3;Parent=MS.gene000446.t1
chr7.1 CDS 68936082 68936683 68936082 ID=cds.MS.gene000446.t1;Parent=MS.gene000446.t1
Gene Sequence

>MS.gene000446

ATGGATTGGGATTGGAAAGAGTTTACTTGGGATCCATCTGGATTGGAGGAGTTGAAAAATGAAGGAGATTCAATGGATTTGAGGCTTGGTGATCAGGCTTCTGATTCATTGGAGAAATCAGTTCATGATTCAGGAAAGGAAGATTCAAAAGCTGTGTCGTCGTCGTCACCTACTAGATCGTTGAAAAGAGCGCGTTTGCAAAATGGATCGCAGAATATGATTTGCTCGGTTGATGGATGTAACTCTGATCTTCGTGATTGTAGAGAGTATCATAAGCGTCATAGGGTGTGCGAAAAGCATTCCAAAACTCCTGTTGTGTTGGTTGGAGGTAAGCAGCAGAGGTTCTGCCAACAATGCAGCAGGTATATGAATTGTAGTTGTTTGTTTCGGGTTAATAACTTGTTGAATTTTGTTGTTTTAGTGTCATGTTTGAATAAACAGCTTAATTAAACGCTTATAGCATTTTTGTTTATCATTACAAGTGTTTATAATTTATGTATTTATGTGTACGTTATTTCTATAACAGTATAAAATAAAGTCAAACTGTTTTCATATAAGCTAGAAGTTGATTTCATAAGCTATTCTGCAAAGAATATGAAAATGAGCTGAAAACAACTTACGGACATGTCATAAGCTGTTTCCATATAGAAGCTATCTGAATATTCTAAAAATGGCTTATTTGAATAGATAAGCTTAAAAATAACAATCCAAACACATCATGTTTTGAGGTTGTTTTGTAACATGGGGTGATGTCAATTTGATGTAGGTTTCATTCACTTGCGGAGTTTGATGAGGTTAAGAGGAGTTGCAGGAAACGTCTTGATGGACATAATAAGCGCAGAAGGAAACCTCAACCACAGTCTCTTTTCATGGCTGCTGAGAAGTTCATGTACAATTACAAAGGTTTGTGTCTTTTGCATCTCATGATTCTAAGATAGATGGCTTATTTCTGTCGATGTTTAACAAGATCTCAGTTTTATGATTGTCAAACCTGAGATGAAAGAGGGAATTATGACGAAGATATAACGTTTTTTATGGAAGAAGATACGCATGATGAATAAGTAAGAAATCACATATACATAACAATCCATAAGGTTTTGACAATAGAATTTAGAGTATCTCTCAAAATTTGTTTCAAAATTTTATTTACCATGAACTGAATTCTGAGCTAAGCTTAATAATTAGCTATAGTTACTTAGCAACCTTTGGATATACAACTTAATTAAAAACTTACTTTAGAAGTGCTTATTGTGAAAGAGTTTATGTTTAAGATGATTCTACTATTGAAGAGGGAATGCATAAAACTCTGCGTAAAGCTAAAATTAGCTTGTGCAATAAGTTTCTAGGAGATGCTTATGCAAATATGTTAAAAGCAACTTAACAAACATGTCGTAATCTATTTTCATTTGCTTTTCCAAATAATCTATGTCATAAGATAAGTTTAAATAAGCGCAAGTGTTTATGTCATAAGATAAGTTTAAATAAGCGCAAGTGTTTATGTCATAAGATAAGTTTAAATAAGCTCTTCCAATCCTCTCTCTAATCAAATTTAATGACAAAGAAAGTGACCTTCCGTCTTTATTTTCACAAGAAAGATGGTGGAGAAATATGAAACTTTGCTAATATTTCCCCATGGATTTCAATTGCAAAAAATGGATGCCCTGCATTTACACGATTAGTGCTTTAGTAACTGTTGGATTTAGATCGGACATTTCACAAAATTTGTTGATTTAATCTTTTCGAAATTCAAAATAGTGAGCTGGAAATTCACACTGTCCAATCTTGATCCAACAGTCACTGATGTGTTGACTGCGTGAATTCATAGTATTCCAACTGCAGGGAATCTTGATCCTTGATTTTCGGCATGCTCAAACTGAACTAATATGATTACTGCTGGCATCTGTTTTTTTTTTTTTTAGTCATGATTTATTACATTATTTTTGTCTTCCACAAGCACAAGTAATATGGAGTTTTAATGCTGTTTTATCTTTGTACAATTTTAGGACCTAGAATCCTGCACTTTGGTAGCCCTCAGACATATGTGAATCCTATTATGAGAAACATTTGGCCTGCAACTTCCATAACTGAAGCTGAATCTGGTTATGATCATCAAAGACTTTTGTATCGAATCGACAAACATAAACAGGATAAGGGACATCCTCATTGGCAAGAAATTCTCCCTAAGTCCGGCGATGTTAACAAAGCAGCACCTGGAACTCCTATCAGCCATCCTATCCGCGGTGCCGTTGGTTCATCCGCAGGTGAAAAAGGTGGTAGGAAGCTCTCTTCTGATGGCAAGCCCGGATCTTTTGACTCGGGTTGTGCTCTCTATCTTCTGTCAACACTTCAAACACAAAGCTCAGAGTTGAGTTTGATGCAGTCAAGCATCAACAACAGTCCAACACAATCTTCGTCTGGAACGTTGCATTTTGATGCTACGAAGGAGTGCTCGTACTCCGGAAAGGCGAAGGATAAGCCGAATGGTCCAGTATTTGTTTTAGATGGAAACACGACAAACCTTCATTGCAATGGGATGCTGCAAATGGGTCCTAATGGATTGATCGAAAATGATAACTCAGTGACTCTTCCATTCTTCTGGGAGTAG
Protein sequence

>MS.gene000446.t1

MDWDWKEFTWDPSGLEELKNEGDSMDLRLGDQASDSLEKSVHDSGKEDSKAVSSSSPTRSLKRARLQNGSQNMICSVDGCNSDLRDCREYHKRHRVCEKHSKTPVVLVGGKQQRFCQQCSRFHSLAEFDEVKRSCRKRLDGHNKRRRKPQPQSLFMAAEKFMYNYKGPRILHFGSPQTYVNPIMRNIWPATSITEAESGYDHQRLLYRIDKHKQDKGHPHWQEILPKSGDVNKAAPGTPISHPIRGAVGSSAGEKGGRKLSSDGKPGSFDSGCALYLLSTLQTQSSELSLMQSSINNSPTQSSSGTLHFDATKECSYSGKAKDKPNGPVFVLDGNTTNLHCNGMLQMGPNGLIENDNSVTLPFFWE