AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000522

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000522.t1 MTR_8g099880 94.361 266 10 2 1 265 66 327 4.45e-165 460
MS.gene000522.t1 MTR_3g498825 60.370 270 91 7 1 265 72 330 5.22e-94 280
MS.gene000522.t1 MTR_7g053410 50.532 188 66 7 22 186 234 417 1.12e-44 157
MS.gene000522.t1 MTR_7g053410 50.532 188 66 7 22 186 234 417 1.59e-44 157
MS.gene000522.t1 MTR_5g037250 54.000 150 60 2 14 154 34 183 1.41e-43 148
MS.gene000522.t1 MTR_1g017350 50.303 165 72 3 12 170 184 344 1.62e-43 152
MS.gene000522.t1 MTR_1g017350 50.303 165 72 3 12 170 184 344 1.95e-43 152
MS.gene000522.t1 MTR_1g017350 50.303 165 72 3 12 170 184 344 1.99e-43 152
MS.gene000522.t1 MTR_1g017350 50.303 165 72 3 12 170 184 344 2.06e-43 152
MS.gene000522.t1 MTR_8g065740 62.500 128 41 3 37 163 263 384 2.21e-41 148
MS.gene000522.t1 MTR_3g116770 82.558 86 14 1 78 163 186 270 5.85e-41 145
MS.gene000522.t1 MTR_8g012290 51.366 183 65 7 11 172 198 377 5.91e-41 147
MS.gene000522.t1 MTR_8g012290 51.366 183 65 7 11 172 198 377 6.05e-41 147
MS.gene000522.t1 MTR_3g116770 82.558 86 14 1 78 163 186 270 6.09e-41 145
MS.gene000522.t1 MTR_8g062240 88.000 75 9 0 80 154 148 222 2.49e-40 140
MS.gene000522.t1 MTR_5g048860 76.289 97 19 3 80 175 362 455 5.00e-40 145
MS.gene000522.t1 MTR_4g070320 82.143 84 14 1 80 163 140 222 6.20e-40 141
MS.gene000522.t1 MTR_1g059270 66.667 102 29 3 68 169 147 243 1.10e-39 138
MS.gene000522.t1 MTR_1g059270 66.667 102 29 3 68 169 147 243 3.94e-39 139
MS.gene000522.t1 MTR_7g092510 61.667 120 37 4 80 194 170 285 1.26e-37 135
MS.gene000522.t1 MTR_6g084120 44.295 149 61 5 22 167 103 232 6.32e-26 102
MS.gene000522.t1 MTR_4g131160 57.831 83 30 2 83 161 196 277 3.20e-21 92.4
MS.gene000522.t1 MTR_4g131160 57.831 83 30 2 83 161 199 280 3.41e-21 92.0
MS.gene000522.t1 MTR_1g080890 65.152 66 22 1 83 148 193 257 2.55e-19 86.3
MS.gene000522.t1 MTR_4g033295 55.385 65 29 0 83 147 118 182 1.08e-15 73.9
MS.gene000522.t1 MTR_4g033295 53.731 67 30 1 83 148 118 184 2.10e-15 72.8
MS.gene000522.t1 MTR_4g065870 56.923 65 28 0 83 147 355 419 3.92e-15 75.1
MS.gene000522.t1 MTR_7g099540 31.034 174 108 4 35 208 212 373 1.43e-14 73.2
MS.gene000522.t1 MTR_4g065870 56.452 62 27 0 83 144 355 416 2.29e-14 72.8
MS.gene000522.t1 MTR_1g069155 36.667 120 63 4 39 158 284 390 4.80e-14 72.0
MS.gene000522.t1 MTR_2g098310 50.746 67 33 0 81 147 330 396 9.02e-14 70.9
MS.gene000522.t1 MTR_1g052470 50.725 69 34 0 83 151 298 366 9.70e-14 70.5
MS.gene000522.t1 MTR_7g092540 46.154 78 39 1 74 151 237 311 1.42e-13 69.7
MS.gene000522.t1 MTR_2g098310 51.515 66 32 0 81 146 330 395 2.74e-13 69.3
MS.gene000522.t1 MTR_1g084980 39.024 82 49 1 67 148 446 526 3.24e-13 69.7
MS.gene000522.t1 MTR_1g070870 38.028 142 72 3 36 162 62 202 5.07e-13 67.8
MS.gene000522.t1 MTR_1g070870 51.190 84 36 2 83 162 123 205 7.17e-13 67.4
MS.gene000522.t1 MTR_7g092540 45.946 74 37 1 74 147 237 307 1.10e-12 67.0
MS.gene000522.t1 MTR_7g099540 30.000 180 108 5 35 208 212 379 2.00e-12 67.0
MS.gene000522.t1 MTR_5g017040 37.719 114 62 4 53 158 68 180 2.32e-12 66.2
MS.gene000522.t1 MTR_4g088710 57.143 56 21 2 213 265 12 67 2.70e-12 61.2
MS.gene000522.t1 MTR_3g028520 49.231 65 33 0 83 147 322 386 4.31e-12 65.9
MS.gene000522.t1 MTR_3g027650 49.231 65 33 0 83 147 322 386 4.31e-12 65.9
MS.gene000522.t1 MTR_1g018090 49.231 65 32 1 83 147 274 337 1.17e-11 64.3
MS.gene000522.t1 MTR_3g027650 50.000 62 31 0 83 144 322 383 1.95e-11 63.9
MS.gene000522.t1 MTR_3g449770 33.094 139 67 5 85 211 311 435 2.98e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000522.t1 AT5G50915 66.929 127 39 1 44 170 100 223 1.65e-55 179
MS.gene000522.t1 AT5G50915 66.929 127 39 1 44 170 100 223 1.65e-55 179
MS.gene000522.t1 AT5G50915 66.929 127 39 1 44 170 100 223 3.20e-55 179
MS.gene000522.t1 AT5G50915 66.929 127 39 1 44 170 100 223 3.20e-55 179
MS.gene000522.t1 AT3G23690 44.398 241 98 9 30 259 132 347 2.70e-47 161
MS.gene000522.t1 AT1G26260 55.280 161 51 5 40 188 117 268 5.95e-47 160
MS.gene000522.t1 AT1G26260 55.280 161 51 5 40 188 146 297 1.25e-46 160
MS.gene000522.t1 AT1G26260 55.280 161 51 5 40 188 168 319 2.01e-46 159
MS.gene000522.t1 AT1G26260 55.280 161 51 5 40 188 168 319 2.01e-46 159
MS.gene000522.t1 AT1G26260 55.280 161 51 5 40 188 168 319 2.01e-46 159
MS.gene000522.t1 AT1G26260 55.280 161 51 5 40 188 168 319 2.01e-46 159
MS.gene000522.t1 AT1G68920 43.162 234 91 7 13 215 218 440 5.56e-45 158
MS.gene000522.t1 AT1G68920 43.162 234 91 7 13 215 218 440 5.56e-45 158
MS.gene000522.t1 AT1G68920 43.162 234 91 7 13 215 218 440 5.56e-45 158
MS.gene000522.t1 AT1G68920 55.152 165 60 6 61 215 279 439 6.74e-45 157
MS.gene000522.t1 AT1G10120 48.889 180 73 5 7 170 114 290 3.61e-44 153
MS.gene000522.t1 AT1G10120 48.889 180 73 5 7 170 114 290 3.61e-44 153
MS.gene000522.t1 AT3G07340 59.589 146 52 3 18 163 199 337 2.63e-43 153
MS.gene000522.t1 AT3G07340 59.589 146 52 3 18 163 199 337 3.07e-43 152
MS.gene000522.t1 AT1G59640 56.552 145 61 2 12 154 63 207 1.05e-42 146
MS.gene000522.t1 AT5G48560 80.899 89 17 0 75 163 293 381 1.11e-42 151
MS.gene000522.t1 AT5G48560 80.899 89 17 0 75 163 293 381 1.86e-42 151
MS.gene000522.t1 AT1G59640 67.826 115 36 1 41 154 93 207 3.41e-42 147
MS.gene000522.t1 AT4G34530 82.558 86 14 1 78 163 167 251 3.20e-41 144
MS.gene000522.t1 AT3G57800 71.875 96 22 3 74 169 197 287 2.03e-38 138
MS.gene000522.t1 AT5G62610 52.740 146 51 3 42 169 101 246 2.38e-38 135
MS.gene000522.t1 AT5G62610 52.740 146 51 3 42 169 101 246 2.38e-38 135
MS.gene000522.t1 AT2G42300 71.111 90 23 2 80 169 182 268 6.21e-38 134
MS.gene000522.t1 AT2G42300 71.111 90 23 2 80 169 182 268 2.77e-37 134
MS.gene000522.t1 AT4G36540 57.500 120 41 2 43 155 97 213 3.72e-37 133
MS.gene000522.t1 AT4G36540 57.500 120 41 2 43 155 97 213 4.15e-37 133
MS.gene000522.t1 AT2G18300 80.263 76 15 0 80 155 182 257 2.34e-36 132
MS.gene000522.t1 AT2G18300 80.263 76 15 0 80 155 182 257 2.60e-36 132
MS.gene000522.t1 AT2G18300 80.263 76 15 0 80 155 182 257 5.96e-36 131
MS.gene000522.t1 AT1G18400 67.816 87 27 1 81 167 143 228 5.98e-35 126
MS.gene000522.t1 AT1G18400 67.816 87 27 1 81 167 143 228 7.27e-35 126
MS.gene000522.t1 AT1G73830 67.442 86 27 1 82 167 146 230 8.60e-34 123
MS.gene000522.t1 AT1G73830 67.442 86 27 1 82 167 146 230 8.62e-34 123
MS.gene000522.t1 AT1G25330 53.448 116 37 2 56 171 89 187 1.32e-33 122
MS.gene000522.t1 AT3G57800 48.951 143 21 4 74 169 197 334 5.01e-30 117
MS.gene000522.t1 AT5G58010 50.962 104 38 3 83 182 99 193 2.82e-21 91.3
MS.gene000522.t1 AT2G24260 62.500 72 26 1 77 148 29 99 3.77e-21 89.7
MS.gene000522.t1 AT4G30980 68.182 66 20 1 83 148 130 194 1.39e-20 89.4
MS.gene000522.t1 AT4G30980 68.182 66 20 1 83 148 178 242 1.48e-20 90.1
MS.gene000522.t1 AT2G24260 59.211 76 30 1 73 148 128 202 2.48e-20 89.4
MS.gene000522.t1 AT2G42300 81.395 43 8 0 80 122 182 224 5.81e-19 83.6
MS.gene000522.t1 AT2G42300 81.395 43 8 0 80 122 182 224 5.81e-19 83.6
MS.gene000522.t1 AT2G42300 81.395 43 8 0 80 122 182 224 6.74e-19 83.6
MS.gene000522.t1 AT4G28815 30.409 171 76 6 86 256 71 198 5.59e-14 69.7
MS.gene000522.t1 AT4G28800 45.833 72 38 1 86 157 252 322 8.71e-14 71.2
MS.gene000522.t1 AT1G35460 50.769 65 32 0 83 147 181 245 1.57e-13 68.9
MS.gene000522.t1 AT4G28815 30.409 171 76 6 86 256 147 274 2.01e-13 69.3
MS.gene000522.t1 AT1G09530 40.449 89 49 2 60 148 317 401 2.10e-13 70.1
MS.gene000522.t1 AT1G09530 40.449 89 49 2 60 148 317 401 2.10e-13 70.1
MS.gene000522.t1 AT1G09530 40.449 89 49 2 60 148 317 401 2.10e-13 70.1
MS.gene000522.t1 AT1G09530 40.449 89 49 2 60 148 317 401 2.10e-13 70.1
MS.gene000522.t1 AT1G09530 40.449 89 49 2 60 148 317 401 2.10e-13 70.1
MS.gene000522.t1 AT1G09530 40.449 89 49 2 60 148 317 401 2.10e-13 70.1
MS.gene000522.t1 AT1G51140 54.688 64 29 0 83 146 304 367 3.00e-13 69.3
MS.gene000522.t1 AT2G20180 49.206 63 31 1 86 148 210 271 4.67e-13 68.9
MS.gene000522.t1 AT2G20180 49.206 63 31 1 86 148 210 271 4.67e-13 68.9
MS.gene000522.t1 AT2G20180 49.206 63 31 1 86 148 210 271 4.67e-13 68.9
MS.gene000522.t1 AT2G20180 49.206 63 31 1 86 148 281 342 4.74e-13 68.9
MS.gene000522.t1 AT2G20180 49.206 63 31 1 86 148 281 342 4.74e-13 68.9
MS.gene000522.t1 AT2G20180 49.206 63 31 1 86 148 281 342 4.74e-13 68.9
MS.gene000522.t1 AT2G20180 49.206 63 31 1 86 148 281 342 4.74e-13 68.9
MS.gene000522.t1 AT4G09180 49.254 67 34 0 81 147 182 248 4.76e-13 67.8
MS.gene000522.t1 AT2G42280 50.000 64 32 0 83 146 279 342 5.00e-13 68.6
MS.gene000522.t1 AT4G28800 42.169 83 44 2 86 165 264 345 5.34e-13 68.9
MS.gene000522.t1 AT2G42280 51.515 66 30 1 83 146 279 344 9.02e-13 67.8
MS.gene000522.t1 AT4G28790 41.892 74 42 1 85 158 273 345 1.28e-12 67.4
MS.gene000522.t1 AT4G02590 50.000 84 37 2 83 162 83 165 1.80e-12 65.9
MS.gene000522.t1 AT4G02590 50.000 84 37 2 83 162 146 228 3.10e-12 65.9
MS.gene000522.t1 AT4G02590 50.000 84 37 2 83 162 146 228 3.10e-12 65.9
MS.gene000522.t1 AT1G03040 59.091 66 26 1 83 148 139 203 1.01e-11 64.3
MS.gene000522.t1 AT1G03040 59.091 66 26 1 83 148 144 208 1.06e-11 64.3
MS.gene000522.t1 AT4G33880 45.783 83 42 3 83 164 266 346 1.33e-11 64.3
MS.gene000522.t1 AT1G03040 59.091 66 26 1 83 148 144 208 1.50e-11 63.9
MS.gene000522.t1 AT2G20180 49.153 59 29 1 86 144 281 338 1.78e-11 63.9
MS.gene000522.t1 AT2G43140 43.590 78 42 1 71 146 120 197 2.53e-11 62.0
MS.gene000522.t1 AT2G43140 43.590 78 42 1 71 146 217 294 3.10e-11 62.8
MS.gene000522.t1 AT1G27740 35.115 131 65 5 29 147 108 230 3.49e-11 62.4
MS.gene000522.t1 AT4G00050 44.595 74 40 1 74 147 96 168 4.06e-11 62.4
MS.gene000522.t1 AT4G00050 46.377 69 36 1 79 147 203 270 9.59e-11 62.0

Find 62 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGAAATTGATATTAATTTA+AGG 0.098761 4.3:+6992919 None:intergenic
AGCACACTGATGGACCATTT+TGG 0.191470 4.3:-6993003 MS.gene000522:CDS
TGTTAATAATGACTATGTTT+TGG 0.279340 4.3:-6993703 MS.gene000522:CDS
TCAGAAAGCTGTTACTTCTA+TGG 0.295160 4.3:-6995064 MS.gene000522:CDS
TTTCATATTAAATAGGTAAC+TGG 0.313052 4.3:-6994188 MS.gene000522:intron
TTTGAGTTGCCCCTTGTCTA+AGG 0.332895 4.3:-6995010 MS.gene000522:intron
CAAAAGCTTCTTAATCCATA+TGG 0.364693 4.3:-6992962 MS.gene000522:CDS
ATTGCAAAGTCAAAGAAGAT+TGG 0.375639 4.3:+6993980 None:intergenic
TATTTCCTCACCTGATGATC+TGG 0.386280 4.3:+6993932 None:intergenic
AAAACATAGTCATTATTAAC+AGG 0.386747 4.3:+6993705 None:intergenic
CTTCTTAATCCATATGGATT+CGG 0.417843 4.3:-6992956 MS.gene000522:CDS
AACTTGTGCCTGGCTGTGAT+AGG 0.422109 4.3:-6994426 MS.gene000522:CDS
TAGCCACAGCCTTGCTGAAA+GGG 0.444848 4.3:-6994735 MS.gene000522:intron
ACATGTGGTATTGGTGTTGA+TGG 0.451696 4.3:+6993789 None:intergenic
CACAATGGTACATGTGGTAT+TGG 0.457363 4.3:+6993780 None:intergenic
AGCAGTGGTGAAGGGATGAA+AGG 0.466227 4.3:-6994857 MS.gene000522:CDS
TGGTCTTCTACGTCCCAAAA+TGG 0.467780 4.3:+6992989 None:intergenic
TTGTAGGATGTCTCAAAAGA+AGG 0.474006 4.3:-6994902 MS.gene000522:intron
GTGTTGTTGCCGAATCCATA+TGG 0.475225 4.3:+6992947 None:intergenic
GGTAGTGGACAATCTTGAAA+AGG 0.476116 4.3:-6995100 MS.gene000522:CDS
AATATTGAAGAAGTTCCAAC+TGG 0.478486 4.3:-6994797 MS.gene000522:CDS
GACCCCTTCTTGCTCTGACA+TGG 0.481977 4.3:+6994768 None:intergenic
CTTGGTTAGACCAGATCATC+AGG 0.485638 4.3:-6993942 MS.gene000522:intron
AATCTTCTTTGACTTTGCAA+TGG 0.497293 4.3:-6993978 MS.gene000522:CDS
ATCAAATTTACCTTAGACAA+GGG 0.503038 4.3:+6995000 None:intergenic
CAAAAGAAGAGCAGTGGTGA+AGG 0.507125 4.3:-6994866 MS.gene000522:CDS
AGCAAAAGGTGTGGCATGGT+TGG 0.509890 4.3:+6993752 None:intergenic
TGTCAGAGCAAGAAGGGGTC+AGG 0.517190 4.3:-6994765 MS.gene000522:CDS
ATATTGCAGCAACTTGTGCC+TGG 0.524669 4.3:-6994436 MS.gene000522:CDS
TGCCGAATCATCCATGGTAG+TGG 0.524946 4.3:-6995115 None:intergenic
GTGGTAATTGTGTCAGCAAA+AGG 0.534827 4.3:+6993738 None:intergenic
ATAGCCACAGCCTTGCTGAA+AGG 0.535109 4.3:-6994736 MS.gene000522:CDS
ATACCCTTTCAGCAAGGCTG+TGG 0.536175 4.3:+6994732 None:intergenic
GAGGTCAAATACCTTCTTTG+AGG 0.536244 4.3:-6993649 MS.gene000522:intron
GATCTAAGGTACCTCAAAGA+AGG 0.549335 4.3:+6993638 None:intergenic
TGTAGGATGTCTCAAAAGAA+GGG 0.553029 4.3:-6994901 MS.gene000522:intron
TATCCATGTCAGAGCAAGAA+GGG 0.554936 4.3:-6994771 MS.gene000522:CDS
GTAGTGGACAATCTTGAAAA+GGG 0.561287 4.3:-6995099 MS.gene000522:CDS
CATAGTCATTATTAACAGGA+TGG 0.563260 4.3:+6993709 None:intergenic
TTCAAGATTGTCCACTACCA+TGG 0.563562 4.3:+6995104 None:intergenic
ACTTGTGCCTGGCTGTGATA+GGG 0.572729 4.3:-6994425 MS.gene000522:intron
AACTGGAAAGGCCCTTGTGT+TGG 0.580713 4.3:-6994171 MS.gene000522:CDS
TGGTGAAGGGATGAAAGGAG+AGG 0.583503 4.3:-6994852 MS.gene000522:CDS
CATCAAATTTACCTTAGACA+AGG 0.584025 4.3:+6994999 None:intergenic
TGATTATTTCATCCAACACA+AGG 0.586530 4.3:+6994159 None:intergenic
AATGGACCTAGACACACTCT+TGG 0.596392 4.3:-6993960 MS.gene000522:CDS
GTCCTTACAAAATCAAGTAG+AGG 0.597808 4.3:-6994126 MS.gene000522:intron
GTCTAACCAAGAGTGTGTCT+AGG 0.610495 4.3:+6993954 None:intergenic
ATATCCATGTCAGAGCAAGA+AGG 0.615381 4.3:-6994772 MS.gene000522:CDS
AAAAGAAGAGCAGTGGTGAA+GGG 0.621109 4.3:-6994865 MS.gene000522:CDS
TATTAAATAGGTAACTGGAA+AGG 0.623233 4.3:-6994183 MS.gene000522:intron
AGACTCACCCTATCACAGCC+AGG 0.626341 4.3:+6994418 None:intergenic
GATTATTTCATCCAACACAA+GGG 0.628005 4.3:+6994160 None:intergenic
TCAAATTTACCTTAGACAAG+GGG 0.638877 4.3:+6995001 None:intergenic
TATTGCATACCCTTTCAGCA+AGG 0.648025 4.3:+6994726 None:intergenic
CTGACATGGATATAACCAGT+TGG 0.650898 4.3:+6994782 None:intergenic
ATTAACAGGATGGAAGCTTG+TGG 0.660676 4.3:+6993719 None:intergenic
ATCCATGTCAGAGCAAGAAG+GGG 0.665171 4.3:-6994770 MS.gene000522:CDS
GAGCTGCACAATGGTACATG+TGG 0.691981 4.3:+6993774 None:intergenic
TGTCAGCAAAAGGTGTGGCA+TGG 0.694030 4.3:+6993748 None:intergenic
GCATGGTTGGAGCTGCACAA+TGG 0.724748 4.3:+6993765 None:intergenic
AATTGTGTCAGCAAAAGGTG+TGG 0.745401 4.3:+6993743 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAATGTTGCAAAAAAAT+TGG - chr4.3:6993534-6993553 MS.gene000522:intron 15.0%
!! ATAGAAACATATTCAAAAAA+GGG + chr4.3:6993074-6993093 None:intergenic 15.0%
!! TAATTGTTGAAGTTATTAAA+GGG + chr4.3:6994552-6994571 None:intergenic 15.0%
!! TACAATGAAAATTATTACAA+GGG + chr4.3:6993691-6993710 None:intergenic 15.0%
!! TATAGAAACATATTCAAAAA+AGG + chr4.3:6993075-6993094 None:intergenic 15.0%
!! TGATACTATATGATTATAAT+TGG - chr4.3:6994427-6994446 MS.gene000522:CDS 15.0%
!! TTAATTGTTGAAGTTATTAA+AGG + chr4.3:6994553-6994572 None:intergenic 15.0%
!! TTTACTCATTAATTATTGTT+TGG + chr4.3:6994594-6994613 None:intergenic 15.0%
!!! AAGTACAACTTTTATATTTT+TGG + chr4.3:6993941-6993960 None:intergenic 15.0%
!! AAAACATAGTCATTATTAAC+AGG + chr4.3:6994331-6994350 None:intergenic 20.0%
!! ATTATTGCAAAATGTTAATC+AGG + chr4.3:6994915-6994934 None:intergenic 20.0%
!! CATTCCTTTAATATAGATAT+CGG - chr4.3:6993639-6993658 MS.gene000522:intron 20.0%
!! CTACAATGAAAATTATTACA+AGG + chr4.3:6993692-6993711 None:intergenic 20.0%
!! TAGGTTATTACAATTTACAA+CGG - chr4.3:6993468-6993487 MS.gene000522:intron 20.0%
!! TATCAAAATCTTATGATCTA+AGG + chr4.3:6994412-6994431 None:intergenic 20.0%
!! TTCTAAATATAAGTCCAATA+CGG - chr4.3:6994521-6994540 MS.gene000522:intron 20.0%
!! TTTCATATTAAATAGGTAAC+TGG - chr4.3:6993845-6993864 MS.gene000522:intron 20.0%
!!! ATTAACATTTTGCAATTTCA+AGG + chr4.3:6994680-6994699 None:intergenic 20.0%
!!! GATTTGGTTTCATATTAAAT+AGG - chr4.3:6993838-6993857 MS.gene000522:intron 20.0%
!!! TAACATTTTGCAATTTTAGA+GGG - chr4.3:6994862-6994881 MS.gene000522:CDS 20.0%
!!! TGTTAATAATGACTATGTTT+TGG - chr4.3:6994330-6994349 MS.gene000522:intron 20.0%
!!! TTAACATTTTGCAATTTTAG+AGG - chr4.3:6994861-6994880 MS.gene000522:CDS 20.0%
!!! TTATTGATTTTGTTACTTTG+TGG - chr4.3:6993105-6993124 MS.gene000522:intron 20.0%
!!! TTGAAATATCGGTTTTAAAT+AGG - chr4.3:6993785-6993804 MS.gene000522:CDS 20.0%
!!! TTTTAAAATTACTACACTGA+TGG + chr4.3:6993392-6993411 None:intergenic 20.0%
!!! TTTTGGTAAAATGACTAAAT+TGG + chr4.3:6994953-6994972 None:intergenic 20.0%
! AAACCGATATTTCAAATTGA+AGG + chr4.3:6993780-6993799 None:intergenic 25.0%
! ATATGAAACCAAATCAAATG+TGG + chr4.3:6993833-6993852 None:intergenic 25.0%
! ATCAAATTTACCTTAGACAA+GGG + chr4.3:6993036-6993055 None:intergenic 25.0%
! ATGTCCGATATCTATATTAA+AGG + chr4.3:6993646-6993665 None:intergenic 25.0%
! ATTACAATTTACAACGGAAA+GGG - chr4.3:6993474-6993493 MS.gene000522:intron 25.0%
! GTTTATAAAGTTAAGCTTGT+TGG + chr4.3:6993984-6994003 None:intergenic 25.0%
! TATTAAATAGGTAACTGGAA+AGG - chr4.3:6993850-6993869 MS.gene000522:intron 25.0%
! TATTACAATTTACAACGGAA+AGG - chr4.3:6993473-6993492 MS.gene000522:intron 25.0%
! TGTATGTATCAATGTTGTAT+CGG - chr4.3:6993719-6993738 MS.gene000522:CDS 25.0%
! TTGCAAAAAAATTGGATTTC+AGG - chr4.3:6993542-6993561 MS.gene000522:intron 25.0%
!! CAACTTCAATTTTTCTTCAA+GGG - chr4.3:6994359-6994378 MS.gene000522:intron 25.0%
!!! GATCAAAGTACTTTATGTTT+TGG + chr4.3:6994970-6994989 None:intergenic 25.0%
AAAAAACAAAAGAAGAGCAG+TGG - chr4.3:6993161-6993180 MS.gene000522:intron 30.0%
AATATTGAAGAAGTTCCAAC+TGG - chr4.3:6993236-6993255 MS.gene000522:intron 30.0%
ACAATGAGTTATGAACTTTG+AGG + chr4.3:6993513-6993532 None:intergenic 30.0%
ATCAACAAGTCTCTACATTA+TGG - chr4.3:6994639-6994658 MS.gene000522:intron 30.0%
ATGAATCTTGATATTCAACG+AGG + chr4.3:6994716-6994735 None:intergenic 30.0%
ATTGCAAAGTCAAAGAAGAT+TGG + chr4.3:6994056-6994075 None:intergenic 30.0%
CAAAAGCTTCTTAATCCATA+TGG - chr4.3:6995071-6995090 MS.gene000522:CDS 30.0%
CATAGTCATTATTAACAGGA+TGG + chr4.3:6994327-6994346 None:intergenic 30.0%
CATCAAATTTACCTTAGACA+AGG + chr4.3:6993037-6993056 None:intergenic 30.0%
CCTTGAAGAAAAATTGAAGT+TGG + chr4.3:6994361-6994380 None:intergenic 30.0%
CTTCTTAATCCATATGGATT+CGG - chr4.3:6995077-6995096 MS.gene000522:CDS 30.0%
GATTATTTCATCCAACACAA+GGG + chr4.3:6993876-6993895 None:intergenic 30.0%
TCAAATTTACCTTAGACAAG+GGG + chr4.3:6993035-6993054 None:intergenic 30.0%
TGATTATTTCATCCAACACA+AGG + chr4.3:6993877-6993896 None:intergenic 30.0%
! AATCTTCTTTGACTTTGCAA+TGG - chr4.3:6994055-6994074 MS.gene000522:intron 30.0%
! ACGCCTTCAATTTGAAATAT+CGG - chr4.3:6993774-6993793 MS.gene000522:CDS 30.0%
! CCAACTTCAATTTTTCTTCA+AGG - chr4.3:6994358-6994377 MS.gene000522:intron 30.0%
! TACCTCTACTTGATTTTGTA+AGG + chr4.3:6993912-6993931 None:intergenic 30.0%
!! AATGTAGAGACTTGTTGATA+AGG + chr4.3:6994638-6994657 None:intergenic 30.0%
!! CGTCTGAATTTGTTATCAAA+TGG - chr4.3:6994741-6994760 MS.gene000522:CDS 30.0%
!! TGTTACTTTGTGGTATTTGT+AGG - chr4.3:6993115-6993134 MS.gene000522:intron 30.0%
!!! GCTAGCTTTTCATCATAATA+GGG + chr4.3:6994997-6995016 None:intergenic 30.0%
!!! GGTTTTAAATAGGTTTACGT+AGG - chr4.3:6993795-6993814 MS.gene000522:CDS 30.0%
!!! TGCTAGCTTTTCATCATAAT+AGG + chr4.3:6994998-6995017 None:intergenic 30.0%
!!! TGTTGAGTTGGTATTTTTAG+TGG + chr4.3:6994800-6994819 None:intergenic 30.0%
CACTAACACCTATCTAGTAT+CGG - chr4.3:6993749-6993768 MS.gene000522:CDS 35.0%
GGAGAAGAAAAAAGAAGAGA+AGG - chr4.3:6993202-6993221 MS.gene000522:intron 35.0%
GTCCTTACAAAATCAAGTAG+AGG - chr4.3:6993907-6993926 MS.gene000522:intron 35.0%
TGTAGGATGTCTCAAAAGAA+GGG - chr4.3:6993132-6993151 MS.gene000522:intron 35.0%
TTGTAGGATGTCTCAAAAGA+AGG - chr4.3:6993131-6993150 MS.gene000522:intron 35.0%
TTTCACAGCCACATTTGATT+TGG - chr4.3:6993822-6993841 MS.gene000522:intron 35.0%
! CTCTGTTACTGAATTTTCAG+AGG - chr4.3:6994203-6994222 MS.gene000522:intron 35.0%
! GATTAAGAAGCTTTTGTCGT+TGG + chr4.3:6995067-6995086 None:intergenic 35.0%
! TATCATTTTCAGCACACTGA+TGG - chr4.3:6995020-6995039 MS.gene000522:CDS 35.0%
! TCAGAAAGCTGTTACTTCTA+TGG - chr4.3:6992969-6992988 MS.gene000522:CDS 35.0%
!! GTAGTGGACAATCTTGAAAA+GGG - chr4.3:6992934-6992953 MS.gene000522:CDS 35.0%
!! TATGAACTTTGAGGCAATTG+AGG + chr4.3:6993504-6993523 None:intergenic 35.0%
AAAAGAAGAGCAGTGGTGAA+GGG - chr4.3:6993168-6993187 MS.gene000522:intron 40.0%
AATCAGATTTGTCCTTGCGT+TGG + chr4.3:6994843-6994862 None:intergenic 40.0%
AATTGTGTCAGCAAAAGGTG+TGG + chr4.3:6994293-6994312 None:intergenic 40.0%
ATATCCATGTCAGAGCAAGA+AGG - chr4.3:6993261-6993280 MS.gene000522:intron 40.0%
ATTAACAGGATGGAAGCTTG+TGG + chr4.3:6994317-6994336 None:intergenic 40.0%
CACAATGGTACATGTGGTAT+TGG + chr4.3:6994256-6994275 None:intergenic 40.0%
CTGACATGGATATAACCAGT+TGG + chr4.3:6993254-6993273 None:intergenic 40.0%
GAGGTCAAATACCTTCTTTG+AGG - chr4.3:6994384-6994403 MS.gene000522:intron 40.0%
GGACTATAACGATGTTGAGT+TGG + chr4.3:6994812-6994831 None:intergenic 40.0%
GTGGTAATTGTGTCAGCAAA+AGG + chr4.3:6994298-6994317 None:intergenic 40.0%
TATCCATGTCAGAGCAAGAA+GGG - chr4.3:6993262-6993281 MS.gene000522:intron 40.0%
TATTGCATACCCTTTCAGCA+AGG + chr4.3:6993310-6993329 None:intergenic 40.0%
TATTTCCTCACCTGATGATC+TGG + chr4.3:6994104-6994123 None:intergenic 40.0%
! AATTGGCTCATGAGATGTCA+GGG - chr4.3:6994444-6994463 MS.gene000522:CDS 40.0%
! ATCAATGTTGTATCGGTGTC+AGG - chr4.3:6993726-6993745 MS.gene000522:CDS 40.0%
! CAATTTTTCTTCAAGGGCAG+AGG - chr4.3:6994365-6994384 MS.gene000522:intron 40.0%
! GATCTAAGGTACCTCAAAGA+AGG + chr4.3:6994398-6994417 None:intergenic 40.0%
! GTCCTTGCGTTGGTATTATA+GGG + chr4.3:6994833-6994852 None:intergenic 40.0%
! GTGTTGTCAGACAAAAGTGT+CGG - chr4.3:6993438-6993457 MS.gene000522:intron 40.0%
! TAATTGGCTCATGAGATGTC+AGG - chr4.3:6994443-6994462 MS.gene000522:CDS 40.0%
! TACTACACTGATGGAAGTGT+CGG + chr4.3:6993383-6993402 None:intergenic 40.0%
! TGTCCTTGCGTTGGTATTAT+AGG + chr4.3:6994834-6994853 None:intergenic 40.0%
!! ACATGTGGTATTGGTGTTGA+TGG + chr4.3:6994247-6994266 None:intergenic 40.0%
!! ATTAAAGGGAAGCACCGTAT+TGG + chr4.3:6994538-6994557 None:intergenic 40.0%
!! GGTAGTGGACAATCTTGAAA+AGG - chr4.3:6992933-6992952 MS.gene000522:CDS 40.0%
AATGGACCTAGACACACTCT+TGG - chr4.3:6994073-6994092 MS.gene000522:intron 45.0%
ATATTGCAGCAACTTGTGCC+TGG - chr4.3:6993597-6993616 MS.gene000522:intron 45.0%
ATCCATGTCAGAGCAAGAAG+GGG - chr4.3:6993263-6993282 MS.gene000522:intron 45.0%
CAAAAGAAGAGCAGTGGTGA+AGG - chr4.3:6993167-6993186 MS.gene000522:intron 45.0%
CGGTGTTTGTGTTTGCGTTA+GGG + chr4.3:6993351-6993370 None:intergenic 45.0%
CTTGGTTAGACCAGATCATC+AGG - chr4.3:6994091-6994110 MS.gene000522:intron 45.0%
GTCCCTATAATACCAACGCA+AGG - chr4.3:6994828-6994847 MS.gene000522:CDS 45.0%
TGGTCTTCTACGTCCCAAAA+TGG + chr4.3:6995047-6995066 None:intergenic 45.0%
TGTTTGCGTTAGGGCTTCAT+AGG + chr4.3:6993342-6993361 None:intergenic 45.0%
TTAGACCAGATCATCAGGTG+AGG - chr4.3:6994096-6994115 MS.gene000522:intron 45.0%
! ATTGGCTCATGAGATGTCAG+GGG - chr4.3:6994445-6994464 MS.gene000522:CDS 45.0%
! GTCTAACCAAGAGTGTGTCT+AGG + chr4.3:6994082-6994101 None:intergenic 45.0%
! GTGTTGTTGCCGAATCCATA+TGG + chr4.3:6995089-6995108 None:intergenic 45.0%
! TCGGTGTTTGTGTTTGCGTT+AGG + chr4.3:6993352-6993371 None:intergenic 45.0%
!! AGCACACTGATGGACCATTT+TGG - chr4.3:6995030-6995049 MS.gene000522:CDS 45.0%
!! CAAAAGTGTCGGAGCTACAT+AGG - chr4.3:6993449-6993468 MS.gene000522:intron 45.0%
!! GCACACTGATGGACCATTTT+GGG - chr4.3:6995031-6995050 MS.gene000522:CDS 45.0%
!! TTTGAGTTGCCCCTTGTCTA+AGG - chr4.3:6993023-6993042 MS.gene000522:intron 45.0%
AACTGGAAAGGCCCTTGTGT+TGG - chr4.3:6993862-6993881 MS.gene000522:intron 50.0%
AACTTGTGCCTGGCTGTGAT+AGG - chr4.3:6993607-6993626 MS.gene000522:intron 50.0%
ACTTGTGCCTGGCTGTGATA+GGG - chr4.3:6993608-6993627 MS.gene000522:intron 50.0%
AGCAGTGGTGAAGGGATGAA+AGG - chr4.3:6993176-6993195 MS.gene000522:intron 50.0%
AGGCGTGACCGATACTAGAT+AGG + chr4.3:6993760-6993779 None:intergenic 50.0%
ATACCCTTTCAGCAAGGCTG+TGG + chr4.3:6993304-6993323 None:intergenic 50.0%
ATAGCCACAGCCTTGCTGAA+AGG - chr4.3:6993297-6993316 MS.gene000522:intron 50.0%
GAGCTGCACAATGGTACATG+TGG + chr4.3:6994262-6994281 None:intergenic 50.0%
GGACTATAACGCTGCTGAGT+TGG - chr4.3:6994883-6994902 MS.gene000522:CDS 50.0%
TAGCCACAGCCTTGCTGAAA+GGG - chr4.3:6993298-6993317 MS.gene000522:intron 50.0%
TGGTGAAGGGATGAAAGGAG+AGG - chr4.3:6993181-6993200 MS.gene000522:intron 50.0%
! AGCAAAAGGTGTGGCATGGT+TGG + chr4.3:6994284-6994303 None:intergenic 50.0%
! TGTCAGCAAAAGGTGTGGCA+TGG + chr4.3:6994288-6994307 None:intergenic 50.0%
!! GGAAGTGTCGGTGTTGTGTT+CGG + chr4.3:6993371-6993390 None:intergenic 50.0%
AGACTCACCCTATCACAGCC+AGG + chr4.3:6993618-6993637 None:intergenic 55.0%
GACCCCTTCTTGCTCTGACA+TGG + chr4.3:6993268-6993287 None:intergenic 55.0%
GCATGGTTGGAGCTGCACAA+TGG + chr4.3:6994271-6994290 None:intergenic 55.0%
TGTCAGAGCAAGAAGGGGTC+AGG - chr4.3:6993268-6993287 MS.gene000522:intron 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 6992931 6995124 6992931 ID=MS.gene000522
chr4.3 mRNA 6992931 6995124 6992931 ID=MS.gene000522.t1;Parent=MS.gene000522
chr4.3 exon 6995011 6995124 6995011 ID=MS.gene000522.t1.exon1;Parent=MS.gene000522.t1
chr4.3 CDS 6995011 6995124 6995011 ID=cds.MS.gene000522.t1;Parent=MS.gene000522.t1
chr4.3 exon 6994736 6994918 6994736 ID=MS.gene000522.t1.exon2;Parent=MS.gene000522.t1
chr4.3 CDS 6994736 6994918 6994736 ID=cds.MS.gene000522.t1;Parent=MS.gene000522.t1
chr4.3 exon 6994426 6994491 6994426 ID=MS.gene000522.t1.exon3;Parent=MS.gene000522.t1
chr4.3 CDS 6994426 6994491 6994426 ID=cds.MS.gene000522.t1;Parent=MS.gene000522.t1
chr4.3 exon 6994127 6994195 6994127 ID=MS.gene000522.t1.exon4;Parent=MS.gene000522.t1
chr4.3 CDS 6994127 6994195 6994127 ID=cds.MS.gene000522.t1;Parent=MS.gene000522.t1
chr4.3 exon 6993943 6994032 6993943 ID=MS.gene000522.t1.exon5;Parent=MS.gene000522.t1
chr4.3 CDS 6993943 6994032 6993943 ID=cds.MS.gene000522.t1;Parent=MS.gene000522.t1
chr4.3 exon 6993650 6993832 6993650 ID=MS.gene000522.t1.exon6;Parent=MS.gene000522.t1
chr4.3 CDS 6993650 6993832 6993650 ID=cds.MS.gene000522.t1;Parent=MS.gene000522.t1
chr4.3 exon 6992931 6993023 6992931 ID=MS.gene000522.t1.exon7;Parent=MS.gene000522.t1
chr4.3 CDS 6992931 6993023 6992931 ID=cds.MS.gene000522.t1;Parent=MS.gene000522.t1
Gene Sequence

>MS.gene000522

ATGGTAGTGGACAATCTTGAAAAGGGTGAACAAGTTACTCAGAAAGCTGTTACTTCTATGGAGAAGAAAAGAAGAATCAGAAATAAGACTTCTTTGAGTTGCCCCTTGTCTAAGGTAAATTTGATGCAGTTTTTTTTTTACCCTTTTTTGAATATGTTTCTATATTGTTTCATGTTATTGATTTTGTTACTTTGTGGTATTTGTAGGATGTCTCAAAAGAAGGGAGAAGCAAAAAACAAAAGAAGAGCAGTGGTGAAGGGATGAAAGGAGAGGAGAAGAAAAAAGAAGAGAAGGATCAAAAGAAAAATATTGAAGAAGTTCCAACTGGTTATATCCATGTCAGAGCAAGAAGGGGTCAGGCAACAGATAGCCACAGCCTTGCTGAAAGGGTATGCAATACAGTTTTCGCCTATGAAGCCCTAACGCAAACACAAACACCGAACACAACACCGACACTTCCATCAGTGTAGTAATTTTAAAAAATTGAATGTAATAACATGTGTCAGTGTGTTGTCAGACAAAAGTGTCGGAGCTACATAGGTTATTACAATTTACAACGGAAAGGGAATTCCTCAATTGCCTCAAAGTTCATAACTCATTGTTAATAAATGTTGCAAAAAAATTGGATTTCAGGTGAGAAGAGAAAAAATAAGTGAAAGAATGAAGATATTGCAGCAACTTGTGCCTGGCTGTGATAGGGTGAGTCTTCATTCCTTTAATATAGATATCGGACATAACATTGATACTGACACATTGACCCTTGTAATAATTTTCATTGTAGAAGTTATTGTATGTATCAATGTTGTATCGGTGTCAGGCACTAACACCTATCTAGTATCGGTCACGCCTTCAATTTGAAATATCGGTTTTAAATAGGTTTACGTAGGCTCTTTTCACAGCCACATTTGATTTGGTTTCATATTAAATAGGTAACTGGAAAGGCCCTTGTGTTGGATGAAATAATCAATTATGTACAGTCCTTACAAAATCAAGTAGAGGTAATGCTGCCAAAAATATAAAAGTTGTACTTTTGAATCATTGATTCATAAACCAACAAGCTTAACTTTATAAACTTCTATATTTCTGATGCAGTTCTTGTCAATGAAACTTGCTTCAGTGAATCCAATCTTCTTTGACTTTGCAATGGACCTAGACACACTCTTGGTTAGACCAGATCATCAGGTGAGGAAATATAACTCAGTAATTAATTTTCATGTACTTGAATTTATATTAGCTGACTCAAACAAATTTATTAATGATGAAATAATGAAACTCTGTTACTGAATTTTCAGAGGTTAAATAGCATAACATCACCATCAACACCAATACCACATGTACCATTGTGCAGCTCCAACCATGCCACACCTTTTGCTGACACAATTACCACAAGCTTCCATCCTGTTAATAATGACTATGTTTTGGATTATCCAACTTCAATTTTTCTTCAAGGGCAGAGGTCAAATACCTTCTTTGAGGTACCTTAGATCATAAGATTTTGATACTATATGATTATAATTGGCTCATGAGATGTCAGGGGATACAATTATGTAATTAGCATACTAGTAAGTTTGATAATTTTTGTCATTATTTTTCTAAATATAAGTCCAATACGGTGCTTCCCTTTAATAACTTCAACAATTAAATATATGTATACAAGTGACCAAACAATAATTAATGAGTAAATATCAAAATCGATCTCTTGCACCTTATCAACAAGTCTCTACATTATGGATATTTTGAAATGATCCTTGAAATTGCAAAATGTTAATCAAGTTTATAACACCTCGTTGAATATCAAGATTCATCTTTACGTCTGAATTTGTTATCAAATGGATCTCTGACATTATTCACTTAGTTCAAAATGAACCACTAAAAATACCAACTCAACATCGTTATAGTCCCTATAATACCAACGCAAGGACAAATCTGATTAACATTTTGCAATTTTAGAGGGACTATAACGCTGCTGAGTTGGTACAAACCTGATTAACATTTTGCAATAATTATTAAGTAAAATATCCAATTTAGTCATTTTACCAAAACATAAAGTACTTTGATCATTTCCCTATTATGATGAAAAGCTAGCATATATCATTTTCAGCACACTGATGGACCATTTTGGGACGTAGAAGACCAACGACAAAAGCTTCTTAATCCATATGGATTCGGCAACAACACATGTTCCTTAAATTAA
Protein sequence

>MS.gene000522.t1

MVVDNLEKGEQVTQKAVTSMEKKRRIRNKTSLSCPLSKDVSKEGRSKKQKKSSGEGMKGEEKKKEEKDQKKNIEEVPTGYIHVRARRGQATDSHSLAERVRREKISERMKILQQLVPGCDRVTGKALVLDEIINYVQSLQNQVEFLSMKLASVNPIFFDFAMDLDTLLVRPDHQRLNSITSPSTPIPHVPLCSSNHATPFADTITTSFHPVNNDYVLDYPTSIFLQGQRSNTFFEHTDGPFWDVEDQRQKLLNPYGFGNNTCSLN