Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000546.t1 | XP_003627264.1 | 66.9 | 254 | 6 | 2 | 1 | 176 | 1 | 254 | 2.40E-78 | 301.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000546.t1 | P10290 | 50.0 | 74 | 18 | 1 | 8 | 62 | 12 | 85 | 9.7e-12 | 71.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000546.t1 | G7L6T6 | 66.9 | 254 | 6 | 2 | 1 | 176 | 1 | 254 | 1.7e-78 | 301.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene000546.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000546.t1 | MTR_8g020490 | 94.783 | 115 | 6 | 0 | 62 | 176 | 140 | 254 | 5.65e-65 | 200 |
| MS.gene000546.t1 | MTR_8g020490 | 74.390 | 82 | 2 | 1 | 1 | 63 | 1 | 82 | 2.04e-32 | 116 |
| MS.gene000546.t1 | MTR_4g125520 | 47.674 | 86 | 22 | 3 | 1 | 63 | 1 | 86 | 1.75e-16 | 75.5 |
| MS.gene000546.t1 | MTR_4g100720 | 44.000 | 75 | 23 | 1 | 8 | 63 | 13 | 87 | 1.47e-13 | 65.5 |
| MS.gene000546.t1 | MTR_3g083540 | 38.667 | 75 | 27 | 1 | 8 | 63 | 41 | 115 | 6.49e-12 | 62.8 |
| MS.gene000546.t1 | MTR_5g079670 | 41.333 | 75 | 25 | 1 | 8 | 63 | 11 | 85 | 1.27e-11 | 61.2 |
| MS.gene000546.t1 | MTR_8g027345 | 40.000 | 75 | 26 | 1 | 8 | 63 | 12 | 86 | 1.35e-11 | 62.0 |
| MS.gene000546.t1 | MTR_4g073420 | 37.209 | 86 | 31 | 2 | 1 | 63 | 1 | 86 | 6.15e-11 | 60.1 |
| MS.gene000546.t1 | MTR_3g097450 | 37.209 | 86 | 31 | 2 | 1 | 63 | 1 | 86 | 6.61e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000546.t1 | AT5G35550 | 44.737 | 76 | 23 | 1 | 7 | 63 | 13 | 88 | 4.00e-14 | 68.6 |
| MS.gene000546.t1 | AT4G34990 | 38.372 | 86 | 30 | 2 | 1 | 63 | 1 | 86 | 1.84e-11 | 61.2 |
| MS.gene000546.t1 | AT5G40330 | 39.286 | 84 | 30 | 2 | 1 | 63 | 3 | 86 | 1.92e-11 | 60.8 |
| MS.gene000546.t1 | AT3G27920 | 37.662 | 77 | 29 | 1 | 6 | 63 | 12 | 88 | 2.47e-11 | 60.5 |
| MS.gene000546.t1 | AT5G14750 | 39.474 | 76 | 27 | 1 | 7 | 63 | 15 | 90 | 3.84e-11 | 59.7 |
| MS.gene000546.t1 | AT5G54230 | 41.333 | 75 | 25 | 1 | 8 | 63 | 12 | 86 | 6.43e-11 | 60.1 |
| MS.gene000546.t1 | AT3G13540 | 37.333 | 75 | 28 | 1 | 8 | 63 | 23 | 97 | 7.99e-11 | 59.3 |
Find 36 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCTTGTGATTCTCCATTTC+TGG | 0.129046 | 8.2:+74497519 | None:intergenic |
| TGTTCATCAATTCAAAATTT+TGG | 0.180669 | 8.2:-74497664 | MS.gene000546:CDS |
| TCCATGAATGACAACATATT+TGG | 0.182285 | 8.2:+74498540 | None:intergenic |
| TTAATTTAGCACCTGCATTT+TGG | 0.200719 | 8.2:+74498494 | None:intergenic |
| CTCCTAGGCTTTGGAGCTTC+TGG | 0.233026 | 8.2:+74497696 | None:intergenic |
| ATAAGACTTCATCGTCTTCT+TGG | 0.253123 | 8.2:-74498094 | MS.gene000546:intron |
| TCCAAATATGTTGTCATTCA+TGG | 0.267011 | 8.2:-74498541 | MS.gene000546:CDS |
| GCCTTCAGTCTCCTAGGCTT+TGG | 0.359484 | 8.2:+74497687 | None:intergenic |
| TGATCATCAAGATCAATTGA+TGG | 0.381636 | 8.2:-74497571 | MS.gene000546:CDS |
| TGACCCTCAAGAGACCCTTT+TGG | 0.399906 | 8.2:-74497427 | MS.gene000546:CDS |
| TAATTCAAAGATTCCAGAAA+TGG | 0.417214 | 8.2:-74497532 | MS.gene000546:CDS |
| TCATTCATGGAGAAGGAAAA+TGG | 0.417247 | 8.2:-74498528 | MS.gene000546:CDS |
| GCACCTTTATTAACCTCCTT+AGG | 0.422387 | 8.2:+74498592 | None:intergenic |
| AGTCCTAAGGAGGTTAATAA+AGG | 0.437320 | 8.2:-74498595 | MS.gene000546:CDS |
| GGATGTGGAGCTTAAGAAGA+TGG | 0.468435 | 8.2:-74497406 | MS.gene000546:CDS |
| CAAAAGGTTGCCCAAAATGC+AGG | 0.474230 | 8.2:-74498505 | MS.gene000546:intron |
| TCTTAAGCTCCACATCCAAA+AGG | 0.487264 | 8.2:+74497412 | None:intergenic |
| TGAACAGCCTTCAGTCTCCT+AGG | 0.499868 | 8.2:+74497681 | None:intergenic |
| CATCCAAAAGGGTCTCTTGA+GGG | 0.509517 | 8.2:+74497424 | None:intergenic |
| TATGATCATGTCCTCTTCAT+CGG | 0.523234 | 8.2:+74498114 | None:intergenic |
| TGGAGAAGGAAAATGGCAAA+AGG | 0.533124 | 8.2:-74498521 | MS.gene000546:CDS |
| CTTAAGCTCCACATCCAAAA+GGG | 0.544118 | 8.2:+74497413 | None:intergenic |
| CACCAGAAGCTCCAAAGCCT+AGG | 0.547207 | 8.2:-74497698 | MS.gene000546:CDS |
| TATGTTGTCATTCATGGAGA+AGG | 0.552003 | 8.2:-74498535 | MS.gene000546:CDS |
| TAACATGGTTAGAAGTCCTA+AGG | 0.553471 | 8.2:-74498608 | None:intergenic |
| AGGAGGTTAATAAAGGTGCT+TGG | 0.559589 | 8.2:-74498588 | MS.gene000546:CDS |
| AAAAGATGACAATGATCAGA+AGG | 0.560801 | 8.2:+74497462 | None:intergenic |
| AGCGGAAGTGAGTATGATCA+AGG | 0.564325 | 8.2:-74497633 | MS.gene000546:CDS |
| CGATATCTCTACCGATGAAG+AGG | 0.571337 | 8.2:-74498125 | MS.gene000546:CDS |
| CTCAAACCAGGTATAAAGAG+AGG | 0.598083 | 8.2:-74498148 | MS.gene000546:intron |
| CATCAAGATCAATTGATGGT+TGG | 0.610901 | 8.2:-74497567 | MS.gene000546:CDS |
| ACATCCAAAAGGGTCTCTTG+AGG | 0.616733 | 8.2:+74497423 | None:intergenic |
| TCCAAAGCCTAGGAGACTGA+AGG | 0.628232 | 8.2:-74497688 | MS.gene000546:CDS |
| CATGGTTAGAAGTCCTAAGG+AGG | 0.660711 | 8.2:-74498605 | MS.gene000546:CDS |
| GAATGACAACATATTTGGAG+AGG | 0.662814 | 8.2:+74498545 | None:intergenic |
| TCCAGAAATGGAGAATCACA+AGG | 0.673085 | 8.2:-74497520 | MS.gene000546:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAGTCATTTATAATAATGA+AGG | + | chr8.2:74498068-74498087 | None:intergenic | 15.0% |
| !! | TGATATTTGTTCTATATAGA+TGG | - | chr8.2:74498094-74498113 | MS.gene000546:intron | 20.0% |
| !!! | TGTTCATCAATTCAAAATTT+TGG | - | chr8.2:74498317-74498336 | MS.gene000546:intron | 20.0% |
| ! | CTAATGCACAACTATATTAT+AGG | - | chr8.2:74497523-74497542 | MS.gene000546:CDS | 25.0% |
| ! | TAATTCAAAGATTCCAGAAA+TGG | - | chr8.2:74498449-74498468 | MS.gene000546:intron | 25.0% |
| ! | TTTCTTAAATAGCCTTCAAT+TGG | - | chr8.2:74497740-74497759 | MS.gene000546:intron | 25.0% |
| !! | TCTAACTATCAAGATTTGTT+TGG | + | chr8.2:74497596-74497615 | None:intergenic | 25.0% |
| !!! | AAAATTTTGGAGAAGAATAG+CGG | - | chr8.2:74498330-74498349 | MS.gene000546:intron | 25.0% |
| AAAAGATGACAATGATCAGA+AGG | + | chr8.2:74498522-74498541 | None:intergenic | 30.0% | |
| ACAATGAAATCAAGAACTAC+TGG | - | chr8.2:74498151-74498170 | MS.gene000546:intron | 30.0% | |
| CAAATCAGATTCGAAATCTA+AGG | + | chr8.2:74497686-74497705 | None:intergenic | 30.0% | |
| CTTAGATTTCGAATCTGATT+TGG | - | chr8.2:74497684-74497703 | MS.gene000546:CDS | 30.0% | |
| TATATGGAGTAGGTTTAAAG+CGG | - | chr8.2:74497774-74497793 | MS.gene000546:intron | 30.0% | |
| TCCAAATATGTTGTCATTCA+TGG | - | chr8.2:74497440-74497459 | MS.gene000546:CDS | 30.0% | |
| TCCATGAATGACAACATATT+TGG | + | chr8.2:74497444-74497463 | None:intergenic | 30.0% | |
| TGATCATCAAGATCAATTGA+TGG | - | chr8.2:74498410-74498429 | MS.gene000546:intron | 30.0% | |
| TGTTGACATAAGTCTCATAA+TGG | - | chr8.2:74497960-74497979 | MS.gene000546:intron | 30.0% | |
| ! | ATTGGTCTCTTTGGTTTATA+TGG | - | chr8.2:74497758-74497777 | MS.gene000546:intron | 30.0% |
| ! | TAATTTAGCACCTGCATTTT+GGG | + | chr8.2:74497489-74497508 | None:intergenic | 30.0% |
| ! | TTAATTTAGCACCTGCATTT+TGG | + | chr8.2:74497490-74497509 | None:intergenic | 30.0% |
| AAACCAAAGAGACCAATTGA+AGG | + | chr8.2:74497755-74497774 | None:intergenic | 35.0% | |
| AGTCCTAAGGAGGTTAATAA+AGG | - | chr8.2:74497386-74497405 | MS.gene000546:CDS | 35.0% | |
| ATAAGACTTCATCGTCTTCT+TGG | - | chr8.2:74497887-74497906 | MS.gene000546:intron | 35.0% | |
| CAACACAATCATCACAAATG+TGG | - | chr8.2:74498252-74498271 | MS.gene000546:intron | 35.0% | |
| CAACGATGATTATTCTAGTC+AGG | - | chr8.2:74498018-74498037 | MS.gene000546:intron | 35.0% | |
| GAAAAAGTTGCAGACAAAGA+TGG | - | chr8.2:74497801-74497820 | MS.gene000546:intron | 35.0% | |
| GAATGACAACATATTTGGAG+AGG | + | chr8.2:74497439-74497458 | None:intergenic | 35.0% | |
| TAAGTCTCATAATGGTGATC+TGG | - | chr8.2:74497968-74497987 | MS.gene000546:intron | 35.0% | |
| TATGATCATGTCCTCTTCAT+CGG | + | chr8.2:74497870-74497889 | None:intergenic | 35.0% | |
| TATGTTGTCATTCATGGAGA+AGG | - | chr8.2:74497446-74497465 | MS.gene000546:CDS | 35.0% | |
| TCATTCATGGAGAAGGAAAA+TGG | - | chr8.2:74497453-74497472 | MS.gene000546:CDS | 35.0% | |
| TCTCATGAAAACACACTGAA+TGG | + | chr8.2:74497552-74497571 | None:intergenic | 35.0% | |
| TGGTTGAATTATCTCAAACC+AGG | - | chr8.2:74497821-74497840 | MS.gene000546:intron | 35.0% | |
| TGTTGAAGTGAAACAGAAGA+TGG | + | chr8.2:74498237-74498256 | None:intergenic | 35.0% | |
| TTTGTTTGGAGAGACATCAA+AGG | + | chr8.2:74497582-74497601 | None:intergenic | 35.0% | |
| ! | AAGAAGATGGCTTCTTTTCT+TGG | - | chr8.2:74498588-74498607 | MS.gene000546:CDS | 35.0% |
| ! | CATCAAGATCAATTGATGGT+TGG | - | chr8.2:74498414-74498433 | MS.gene000546:intron | 35.0% |
| ! | CTCTTTGGTTTATATGGAGT+AGG | - | chr8.2:74497764-74497783 | MS.gene000546:intron | 35.0% |
| ACCTTGTGATTCTCCATTTC+TGG | + | chr8.2:74498465-74498484 | None:intergenic | 40.0% | |
| AGTGAAACAGAAGATGGTGA+TGG | + | chr8.2:74498231-74498250 | None:intergenic | 40.0% | |
| ATATCGCCTCTCTTTATACC+TGG | + | chr8.2:74497842-74497861 | None:intergenic | 40.0% | |
| ATTTCATTGTCTGTTCGTCC+TGG | + | chr8.2:74498141-74498160 | None:intergenic | 40.0% | |
| CTCAAACCAGGTATAAAGAG+AGG | - | chr8.2:74497833-74497852 | MS.gene000546:intron | 40.0% | |
| CTTAAGCTCCACATCCAAAA+GGG | + | chr8.2:74498571-74498590 | None:intergenic | 40.0% | |
| GAACAATCTTGTATCCTCTG+CGG | + | chr8.2:74497654-74497673 | None:intergenic | 40.0% | |
| GCACCTTTATTAACCTCCTT+AGG | + | chr8.2:74497392-74497411 | None:intergenic | 40.0% | |
| TCCAGAAATGGAGAATCACA+AGG | - | chr8.2:74498461-74498480 | MS.gene000546:intron | 40.0% | |
| TCGAAATCTAAGGACATGCA+TGG | + | chr8.2:74497676-74497695 | None:intergenic | 40.0% | |
| TCTTAAGCTCCACATCCAAA+AGG | + | chr8.2:74498572-74498591 | None:intergenic | 40.0% | |
| TGGAGAAGGAAAATGGCAAA+AGG | - | chr8.2:74497460-74497479 | MS.gene000546:CDS | 40.0% | |
| ! | CATAATGGTGATCTGGAGTT+CGG | - | chr8.2:74497975-74497994 | MS.gene000546:intron | 40.0% |
| ! | GATGGTCATTGATAGCTAAG+AGG | - | chr8.2:74498112-74498131 | MS.gene000546:CDS | 40.0% |
| ! | GGCAATGATGATGATGATGT+TGG | + | chr8.2:74498210-74498229 | None:intergenic | 40.0% |
| ! | TAGCCTTCAATTGGTCTCTT+TGG | - | chr8.2:74497749-74497768 | MS.gene000546:intron | 40.0% |
| ! | TTCATCGTCTTCTTGGTAAC+AGG | - | chr8.2:74497894-74497913 | MS.gene000546:intron | 40.0% |
| !! | AGGAGGTTAATAAAGGTGCT+TGG | - | chr8.2:74497393-74497412 | MS.gene000546:CDS | 40.0% |
| ACATCCAAAAGGGTCTCTTG+AGG | + | chr8.2:74498561-74498580 | None:intergenic | 45.0% | |
| AGCGGAAGTGAGTATGATCA+AGG | - | chr8.2:74498348-74498367 | MS.gene000546:intron | 45.0% | |
| CAAAAGGTTGCCCAAAATGC+AGG | - | chr8.2:74497476-74497495 | MS.gene000546:CDS | 45.0% | |
| CATCCAAAAGGGTCTCTTGA+GGG | + | chr8.2:74498560-74498579 | None:intergenic | 45.0% | |
| CGATATCTCTACCGATGAAG+AGG | - | chr8.2:74497856-74497875 | MS.gene000546:intron | 45.0% | |
| GACAACATGATCTTCTGCCA+CGG | + | chr8.2:74497710-74497729 | None:intergenic | 45.0% | |
| GATGAATTTGAACGTCCGAG+AGG | - | chr8.2:74497614-74497633 | MS.gene000546:CDS | 45.0% | |
| GGATGTGGAGCTTAAGAAGA+TGG | - | chr8.2:74498575-74498594 | MS.gene000546:CDS | 45.0% | |
| ! | TCAAGAGACCCTTTTGGATG+TGG | - | chr8.2:74498560-74498579 | MS.gene000546:CDS | 45.0% |
| ! | TTGATAGCTAAGAGGCTACC+AGG | - | chr8.2:74498120-74498139 | MS.gene000546:CDS | 45.0% |
| !! | TTTCGAATCTGATTTGGCCG+TGG | - | chr8.2:74497690-74497709 | MS.gene000546:CDS | 45.0% |
| GTCTCTCCAGACAAACCTCT+CGG | + | chr8.2:74497632-74497651 | None:intergenic | 50.0% | |
| TCCAAAGCCTAGGAGACTGA+AGG | - | chr8.2:74498293-74498312 | MS.gene000546:intron | 50.0% | |
| TGAACAGCCTTCAGTCTCCT+AGG | + | chr8.2:74498303-74498322 | None:intergenic | 50.0% | |
| ! | GGAGTAGGTTTAAAGCGGTG+TGG | - | chr8.2:74497779-74497798 | MS.gene000546:intron | 50.0% |
| ! | TGACCCTCAAGAGACCCTTT+TGG | - | chr8.2:74498554-74498573 | MS.gene000546:CDS | 50.0% |
| CACCAGAAGCTCCAAAGCCT+AGG | - | chr8.2:74498283-74498302 | MS.gene000546:intron | 55.0% | |
| GAACGTCCGAGAGGTTTGTC+TGG | - | chr8.2:74497623-74497642 | MS.gene000546:CDS | 55.0% | |
| GCCTTCAGTCTCCTAGGCTT+TGG | + | chr8.2:74498297-74498316 | None:intergenic | 55.0% | |
| TTTGTCTGGAGAGACCGCAG+AGG | - | chr8.2:74497637-74497656 | MS.gene000546:CDS | 55.0% | |
| !! | CTCCTAGGCTTTGGAGCTTC+TGG | + | chr8.2:74498288-74498307 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 74497377 | 74498626 | 74497377 | ID=MS.gene000546 |
| chr8.2 | mRNA | 74497377 | 74498626 | 74497377 | ID=MS.gene000546.t1;Parent=MS.gene000546 |
| chr8.2 | exon | 74498506 | 74498626 | 74498506 | ID=MS.gene000546.t1.exon1;Parent=MS.gene000546.t1 |
| chr8.2 | CDS | 74498506 | 74498626 | 74498506 | ID=cds.MS.gene000546.t1;Parent=MS.gene000546.t1 |
| chr8.2 | exon | 74498095 | 74498160 | 74498095 | ID=MS.gene000546.t1.exon2;Parent=MS.gene000546.t1 |
| chr8.2 | CDS | 74498095 | 74498160 | 74498095 | ID=cds.MS.gene000546.t1;Parent=MS.gene000546.t1 |
| chr8.2 | exon | 74497377 | 74497720 | 74497377 | ID=MS.gene000546.t1.exon3;Parent=MS.gene000546.t1 |
| chr8.2 | CDS | 74497377 | 74497720 | 74497377 | ID=cds.MS.gene000546.t1;Parent=MS.gene000546.t1 |
| Gene Sequence |
| Protein sequence |
