Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000702.t1 | AES77546.1 | 91.8 | 159 | 13 | 0 | 1 | 159 | 1 | 159 | 7.90E-73 | 283.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000702.t1 | Q9FJK3 | 47.4 | 156 | 81 | 1 | 1 | 155 | 1 | 156 | 4.9e-31 | 135.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000702.t1 | G7L356 | 91.8 | 159 | 13 | 0 | 1 | 159 | 1 | 159 | 5.7e-73 | 283.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene000702.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000702.t1 | MTR_7g011950 | 91.824 | 159 | 13 | 0 | 1 | 159 | 1 | 159 | 3.25e-98 | 280 |
| MS.gene000702.t1 | MTR_4g028720 | 90.566 | 159 | 15 | 0 | 1 | 159 | 1 | 159 | 3.19e-95 | 272 |
| MS.gene000702.t1 | MTR_1g077320 | 83.648 | 159 | 26 | 0 | 1 | 159 | 1 | 159 | 3.85e-88 | 254 |
| MS.gene000702.t1 | MTR_5g047580 | 69.872 | 156 | 47 | 0 | 1 | 156 | 1 | 156 | 5.71e-75 | 221 |
| MS.gene000702.t1 | MTR_1g077390 | 68.790 | 157 | 49 | 0 | 1 | 157 | 1 | 157 | 9.13e-74 | 218 |
| MS.gene000702.t1 | MTR_1g084950 | 75.000 | 156 | 39 | 0 | 1 | 156 | 1 | 156 | 2.53e-73 | 217 |
| MS.gene000702.t1 | MTR_1g077300 | 74.843 | 159 | 38 | 1 | 1 | 159 | 1 | 157 | 1.19e-72 | 215 |
| MS.gene000702.t1 | MTR_5g047560 | 59.615 | 156 | 47 | 1 | 1 | 156 | 1 | 140 | 1.38e-57 | 176 |
| MS.gene000702.t1 | MTR_1g090697 | 53.548 | 155 | 72 | 0 | 1 | 155 | 1 | 155 | 9.17e-56 | 172 |
| MS.gene000702.t1 | MTR_1g090783 | 55.556 | 153 | 68 | 0 | 3 | 155 | 2 | 154 | 1.58e-55 | 172 |
| MS.gene000702.t1 | MTR_1g090710 | 51.282 | 156 | 75 | 1 | 1 | 155 | 1 | 156 | 2.91e-53 | 166 |
| MS.gene000702.t1 | MTR_3g466980 | 45.752 | 153 | 82 | 1 | 1 | 152 | 1 | 153 | 4.27e-41 | 137 |
| MS.gene000702.t1 | MTR_4g032620 | 40.645 | 155 | 90 | 2 | 1 | 153 | 1 | 155 | 1.58e-40 | 136 |
| MS.gene000702.t1 | MTR_3g065100 | 48.408 | 157 | 80 | 1 | 1 | 156 | 1 | 157 | 9.18e-39 | 132 |
| MS.gene000702.t1 | MTR_5g075380 | 36.538 | 156 | 98 | 1 | 1 | 155 | 1 | 156 | 2.45e-34 | 118 |
| MS.gene000702.t1 | MTR_3g466830 | 42.038 | 157 | 90 | 1 | 3 | 158 | 2 | 158 | 4.72e-34 | 117 |
| MS.gene000702.t1 | MTR_8g036130 | 40.252 | 159 | 91 | 2 | 1 | 155 | 1 | 159 | 6.96e-34 | 119 |
| MS.gene000702.t1 | MTR_4g063790 | 37.736 | 159 | 97 | 2 | 1 | 157 | 1 | 159 | 1.12e-32 | 114 |
| MS.gene000702.t1 | MTR_3g466890 | 40.260 | 154 | 91 | 1 | 3 | 155 | 2 | 155 | 4.99e-31 | 110 |
| MS.gene000702.t1 | MTR_3g467080 | 35.220 | 159 | 101 | 2 | 1 | 157 | 1 | 159 | 6.78e-30 | 107 |
| MS.gene000702.t1 | MTR_4g031910 | 41.333 | 150 | 86 | 2 | 1 | 148 | 1 | 150 | 7.93e-30 | 108 |
| MS.gene000702.t1 | MTR_2g016210 | 38.994 | 159 | 95 | 2 | 1 | 157 | 1 | 159 | 1.79e-29 | 108 |
| MS.gene000702.t1 | MTR_4g032260 | 41.250 | 160 | 92 | 2 | 1 | 158 | 1 | 160 | 3.65e-29 | 105 |
| MS.gene000702.t1 | MTR_3g031240 | 35.849 | 159 | 100 | 2 | 1 | 157 | 1 | 159 | 1.41e-27 | 101 |
| MS.gene000702.t1 | MTR_4g032290 | 38.509 | 161 | 97 | 2 | 1 | 159 | 1 | 161 | 1.91e-26 | 100 |
| MS.gene000702.t1 | MTR_3g031100 | 36.709 | 158 | 96 | 3 | 1 | 155 | 1 | 157 | 2.57e-26 | 100 |
| MS.gene000702.t1 | MTR_2g035580 | 35.484 | 155 | 99 | 1 | 1 | 154 | 1 | 155 | 7.31e-25 | 94.0 |
| MS.gene000702.t1 | MTR_2g035610 | 41.525 | 118 | 68 | 1 | 1 | 117 | 1 | 118 | 1.20e-23 | 89.7 |
| MS.gene000702.t1 | MTR_4g028800 | 49.315 | 73 | 35 | 1 | 1 | 73 | 1 | 71 | 3.62e-22 | 84.7 |
| MS.gene000702.t1 | MTR_3g466900 | 38.400 | 125 | 76 | 1 | 32 | 155 | 2 | 126 | 7.53e-22 | 85.5 |
| MS.gene000702.t1 | MTR_3g466930 | 35.200 | 125 | 80 | 1 | 32 | 155 | 2 | 126 | 3.05e-19 | 79.0 |
| MS.gene000702.t1 | MTR_3g465410 | 33.588 | 131 | 85 | 2 | 29 | 157 | 1 | 131 | 3.37e-19 | 79.0 |
| MS.gene000702.t1 | MTR_4g019670 | 36.800 | 125 | 71 | 4 | 1 | 121 | 1 | 121 | 7.70e-17 | 77.0 |
| MS.gene000702.t1 | MTR_7g106510 | 33.333 | 114 | 72 | 2 | 3 | 115 | 6 | 116 | 3.37e-16 | 75.1 |
| MS.gene000702.t1 | MTR_7g055800 | 33.913 | 115 | 49 | 2 | 1 | 115 | 1 | 88 | 4.13e-14 | 64.3 |
| MS.gene000702.t1 | MTR_6g005450 | 35.789 | 95 | 55 | 3 | 1 | 89 | 1 | 95 | 3.68e-13 | 65.9 |
| MS.gene000702.t1 | MTR_1g033370 | 43.617 | 94 | 52 | 1 | 64 | 156 | 28 | 121 | 4.07e-13 | 62.8 |
| MS.gene000702.t1 | MTR_1g033930 | 39.796 | 98 | 58 | 1 | 62 | 158 | 20 | 117 | 7.21e-13 | 62.0 |
| MS.gene000702.t1 | MTR_6g018920 | 35.000 | 80 | 46 | 3 | 1 | 74 | 1 | 80 | 1.50e-12 | 64.3 |
| MS.gene000702.t1 | MTR_7g055940 | 34.615 | 104 | 41 | 3 | 1 | 104 | 1 | 77 | 2.60e-12 | 59.3 |
| MS.gene000702.t1 | MTR_3g093900 | 30.973 | 113 | 76 | 1 | 3 | 115 | 6 | 116 | 4.90e-12 | 63.2 |
| MS.gene000702.t1 | MTR_7g055790 | 36.697 | 109 | 52 | 2 | 1 | 109 | 59 | 150 | 6.37e-12 | 60.1 |
| MS.gene000702.t1 | MTR_1g033790 | 37.168 | 113 | 64 | 2 | 53 | 158 | 5 | 117 | 8.32e-12 | 59.3 |
| MS.gene000702.t1 | MTR_1g114730 | 33.750 | 80 | 47 | 2 | 1 | 74 | 1 | 80 | 9.58e-12 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000702.t1 | AT5G48670 | 47.712 | 153 | 79 | 1 | 1 | 152 | 1 | 153 | 9.48e-36 | 126 |
| MS.gene000702.t1 | AT3G05860 | 40.940 | 149 | 87 | 1 | 1 | 148 | 1 | 149 | 1.07e-33 | 118 |
| MS.gene000702.t1 | AT3G05860 | 40.940 | 149 | 87 | 1 | 1 | 148 | 1 | 149 | 7.20e-33 | 117 |
| MS.gene000702.t1 | AT3G05860 | 40.940 | 149 | 87 | 1 | 1 | 148 | 1 | 149 | 9.78e-33 | 117 |
| MS.gene000702.t1 | AT5G26630 | 39.375 | 160 | 96 | 1 | 1 | 159 | 1 | 160 | 1.02e-28 | 105 |
| MS.gene000702.t1 | AT1G65300 | 37.324 | 142 | 88 | 1 | 3 | 143 | 2 | 143 | 1.36e-27 | 104 |
| MS.gene000702.t1 | AT1G65330 | 37.415 | 147 | 91 | 1 | 3 | 148 | 2 | 148 | 5.26e-27 | 102 |
| MS.gene000702.t1 | AT5G26650 | 35.294 | 153 | 98 | 1 | 4 | 155 | 2 | 154 | 3.63e-25 | 99.4 |
| MS.gene000702.t1 | AT5G27960 | 34.641 | 153 | 99 | 1 | 4 | 155 | 2 | 154 | 6.12e-24 | 95.5 |
| MS.gene000702.t1 | AT5G27810 | 44.792 | 96 | 52 | 1 | 29 | 123 | 1 | 96 | 2.57e-22 | 86.3 |
| MS.gene000702.t1 | AT1G22590 | 41.304 | 92 | 47 | 1 | 1 | 85 | 1 | 92 | 1.79e-21 | 85.5 |
| MS.gene000702.t1 | AT2G28700 | 32.886 | 149 | 99 | 1 | 1 | 148 | 1 | 149 | 1.88e-21 | 89.0 |
| MS.gene000702.t1 | AT1G31630 | 29.677 | 155 | 107 | 2 | 3 | 156 | 2 | 155 | 3.68e-21 | 88.2 |
| MS.gene000702.t1 | AT1G31640 | 32.000 | 150 | 100 | 2 | 3 | 151 | 2 | 150 | 4.07e-19 | 83.2 |
| MS.gene000702.t1 | AT5G26580 | 32.903 | 155 | 91 | 1 | 1 | 155 | 1 | 142 | 1.02e-17 | 79.0 |
| MS.gene000702.t1 | AT5G06500 | 30.818 | 159 | 106 | 3 | 1 | 157 | 1 | 157 | 6.09e-15 | 70.1 |
| MS.gene000702.t1 | AT2G40210 | 38.835 | 103 | 58 | 2 | 1 | 100 | 1 | 101 | 8.80e-15 | 70.9 |
| MS.gene000702.t1 | AT5G58890 | 37.500 | 96 | 53 | 2 | 1 | 89 | 1 | 96 | 2.02e-14 | 69.3 |
| MS.gene000702.t1 | AT5G55690 | 35.802 | 81 | 45 | 2 | 1 | 74 | 1 | 81 | 8.37e-12 | 62.0 |
| MS.gene000702.t1 | AT5G55690 | 35.802 | 81 | 45 | 2 | 1 | 74 | 1 | 81 | 8.37e-12 | 62.0 |
Find 26 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCCTTTCTTGTTGAATCAT+TGG | 0.233760 | 7.1:-49024519 | None:intergenic |
| TGTTGAATCATTGGAAATAA+AGG | 0.251603 | 7.1:-49024510 | None:intergenic |
| AGCAGAACATTCTTATGTTT+TGG | 0.267458 | 7.1:+49024816 | MS.gene000702:CDS |
| ACCTTTCTTCCTCCTCTTGT+AGG | 0.274122 | 7.1:-49024543 | None:intergenic |
| TGTTTGGCTTCCTTTAGATT+TGG | 0.353502 | 7.1:-49024659 | None:intergenic |
| ATACCTCTCAATCACCTGTT+TGG | 0.354870 | 7.1:-49024675 | None:intergenic |
| TTCCAATGATTCAACAAGAA+AGG | 0.389550 | 7.1:+49024517 | MS.gene000702:CDS |
| TTTGCAAAAGGAAACTTTCT+CGG | 0.420759 | 7.1:-49024736 | None:intergenic |
| AAATCTAAAGGAAGCCAAAC+AGG | 0.425294 | 7.1:+49024661 | MS.gene000702:CDS |
| TCAGAAACAGTCACATCATC+TGG | 0.447805 | 7.1:-49024860 | None:intergenic |
| CTTTAGTAATCATTTGCAAA+AGG | 0.449651 | 7.1:-49024748 | None:intergenic |
| ACCTACAAGAGGAGGAAGAA+AGG | 0.464332 | 7.1:+49024542 | MS.gene000702:CDS |
| TGTTTCTGATTTGAAAGACT+TGG | 0.473410 | 7.1:+49024874 | MS.gene000702:CDS |
| TTTCACCTTCTTCCTAGTCA+TGG | 0.490416 | 7.1:-49024483 | None:intergenic |
| GCAAATGATTACTAAAGCTA+CGG | 0.498143 | 7.1:+49024754 | MS.gene000702:CDS |
| ACCTCTGTTTCAGAATCAAA+AGG | 0.516061 | 7.1:-49024632 | None:intergenic |
| AGAGGTGTGGCCAAATCTAA+AGG | 0.522555 | 7.1:+49024649 | MS.gene000702:CDS |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 7.1:+49024581 | MS.gene000702:CDS |
| CAAAGCCATGACTAGGAAGA+AGG | 0.543154 | 7.1:+49024478 | None:intergenic |
| GAAGGAAATTGATGACAAGA+TGG | 0.549956 | 7.1:+49024919 | MS.gene000702:CDS |
| GAAAGGAGACCTACAAGAGG+AGG | 0.585792 | 7.1:+49024534 | MS.gene000702:CDS |
| GAAGAAAGGTATCATCAAGA+AGG | 0.595296 | 7.1:+49024556 | MS.gene000702:CDS |
| TACATTGCAAAGCCATGACT+AGG | 0.609749 | 7.1:+49024471 | None:intergenic |
| TTGATTCTGAAACAGAGGTG+TGG | 0.630350 | 7.1:+49024636 | MS.gene000702:CDS |
| AAGCCAAACAGGTGATTGAG+AGG | 0.637552 | 7.1:+49024672 | MS.gene000702:CDS |
| CAAGAAAGGAGACCTACAAG+AGG | 0.685284 | 7.1:+49024531 | MS.gene000702:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTAATTGAAAAAAATATGA+AGG | + | chr7.1:49024901-49024920 | MS.gene000702:CDS | 15.0% |
| ! | CTTTAGTAATCATTTGCAAA+AGG | - | chr7.1:49024751-49024770 | None:intergenic | 25.0% |
| ! | TGTTGAATCATTGGAAATAA+AGG | - | chr7.1:49024513-49024532 | None:intergenic | 25.0% |
| GCAAATGATTACTAAAGCTA+CGG | + | chr7.1:49024754-49024773 | MS.gene000702:CDS | 30.0% | |
| TTCCAATGATTCAACAAGAA+AGG | + | chr7.1:49024517-49024536 | MS.gene000702:CDS | 30.0% | |
| TTTGCAAAAGGAAACTTTCT+CGG | - | chr7.1:49024739-49024758 | None:intergenic | 30.0% | |
| ! | AGCAGAACATTCTTATGTTT+TGG | + | chr7.1:49024816-49024835 | MS.gene000702:CDS | 30.0% |
| ! | TGTTTCTGATTTGAAAGACT+TGG | + | chr7.1:49024874-49024893 | MS.gene000702:CDS | 30.0% |
| AAATCTAAAGGAAGCCAAAC+AGG | + | chr7.1:49024661-49024680 | MS.gene000702:CDS | 35.0% | |
| ACCTCTGTTTCAGAATCAAA+AGG | - | chr7.1:49024635-49024654 | None:intergenic | 35.0% | |
| AGAGAACTCACTATTCTTTG+TGG | + | chr7.1:49024581-49024600 | MS.gene000702:CDS | 35.0% | |
| CTCCTTTCTTGTTGAATCAT+TGG | - | chr7.1:49024522-49024541 | None:intergenic | 35.0% | |
| ! | GAAGGAAATTGATGACAAGA+TGG | + | chr7.1:49024919-49024938 | MS.gene000702:CDS | 35.0% |
| ! | TGTTTGGCTTCCTTTAGATT+TGG | - | chr7.1:49024662-49024681 | None:intergenic | 35.0% |
| !! | GAAGAAAGGTATCATCAAGA+AGG | + | chr7.1:49024556-49024575 | MS.gene000702:CDS | 35.0% |
| !!! | TCCTTTTGATTCTGAAACAG+AGG | + | chr7.1:49024631-49024650 | MS.gene000702:CDS | 35.0% |
| ATACCTCTCAATCACCTGTT+TGG | - | chr7.1:49024678-49024697 | None:intergenic | 40.0% | |
| TCAGAAACAGTCACATCATC+TGG | - | chr7.1:49024863-49024882 | None:intergenic | 40.0% | |
| !! | TTGATTCTGAAACAGAGGTG+TGG | + | chr7.1:49024636-49024655 | MS.gene000702:CDS | 40.0% |
| AAGCCAAACAGGTGATTGAG+AGG | + | chr7.1:49024672-49024691 | MS.gene000702:CDS | 45.0% | |
| ACCTACAAGAGGAGGAAGAA+AGG | + | chr7.1:49024542-49024561 | MS.gene000702:CDS | 45.0% | |
| ACCTTTCTTCCTCCTCTTGT+AGG | - | chr7.1:49024546-49024565 | None:intergenic | 45.0% | |
| CAAGAAAGGAGACCTACAAG+AGG | + | chr7.1:49024531-49024550 | MS.gene000702:CDS | 45.0% | |
| ! | AGAGGTGTGGCCAAATCTAA+AGG | + | chr7.1:49024649-49024668 | MS.gene000702:CDS | 45.0% |
| GAAAGGAGACCTACAAGAGG+AGG | + | chr7.1:49024534-49024553 | MS.gene000702:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 49024485 | 49024964 | 49024485 | ID=MS.gene000702 |
| chr7.1 | mRNA | 49024485 | 49024964 | 49024485 | ID=MS.gene000702.t1;Parent=MS.gene000702 |
| chr7.1 | exon | 49024485 | 49024964 | 49024485 | ID=MS.gene000702.t1.exon1;Parent=MS.gene000702.t1 |
| chr7.1 | CDS | 49024485 | 49024964 | 49024485 | ID=cds.MS.gene000702.t1;Parent=MS.gene000702.t1 |
| Gene Sequence |
| Protein sequence |
