AlfalfaGEDB | MS.gene000729

M. sativa cultivar XinJiangDaYe / MS.gene000729

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000729.t1	XP_003616375.2	95.8	311	13	0	1	311	1	311	6.40E-143	516.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000729.t1	Q9C707	76.4	106	24	1	207	311	183	288	7.1e-34	146.0

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000729.t1	G7K1Y5	95.8	311	13	0	1	311	1	311	4.6e-143	516.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene000729.t1	TF	bHLH

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000729.t1	MTR_5g079500	96.785	311	10	0	1	311	1	311	0.0	627
MS.gene000729.t1	MTR_2g021380	93.902	82	4	1	18	99	1	81	1.11e-46	154
MS.gene000729.t1	MTR_3g073460	85.714	84	8	3	18	99	1	82	5.48e-35	123
MS.gene000729.t1	MTR_5g005110	62.500	72	27	0	215	286	221	292	2.64e-25	103
MS.gene000729.t1	MTR_1g106470	50.000	94	41	2	197	286	173	264	2.04e-24	100
MS.gene000729.t1	MTR_1g106460	46.667	120	51	3	167	286	200	306	9.44e-24	99.4
MS.gene000729.t1	MTR_3g103030	58.571	70	29	0	217	286	228	297	1.54e-22	95.9
MS.gene000729.t1	MTR_1g018090	62.903	62	23	0	225	286	281	342	2.64e-22	95.9
MS.gene000729.t1	MTR_8g093500	53.165	79	35	2	202	280	100	176	5.95e-19	85.1
MS.gene000729.t1	MTR_3g101810	62.500	56	21	0	225	280	178	233	2.86e-18	83.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene000729.t1	AT5G37800	53.439	189	66	5	130	311	121	294	7.82e-54	178
MS.gene000729.t1	AT1G66470	61.905	147	49	2	163	309	147	286	1.57e-50	169
MS.gene000729.t1	AT2G14760	60.000	70	28	0	217	286	236	305	4.42e-24	100
MS.gene000729.t1	AT4G33880	60.000	70	28	0	217	286	265	334	8.33e-24	100
MS.gene000729.t1	AT1G27740	54.430	79	36	0	208	286	157	235	1.67e-23	97.4
MS.gene000729.t1	AT5G43175	58.442	77	30	1	212	286	124	200	1.89e-23	96.3
MS.gene000729.t1	AT2G14760	50.602	83	28	1	217	286	236	318	1.38e-20	90.9
MS.gene000729.t1	AT5G01305	57.576	66	28	0	212	277	31	96	5.04e-20	85.9
MS.gene000729.t1	AT2G24260	50.000	86	39	1	217	302	34	115	1.98e-19	86.3
MS.gene000729.t1	AT2G24260	50.000	86	39	1	217	302	137	218	1.18e-18	85.5

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Find 45 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCTACCTTGGAACCGTT+TGG	0.425715	5.2:-59956972	None:intergenic
AAGTAACACAAACTTGTGCA+TGG	0.624267	5.2:+59956207	MS.gene000729:CDS
AATAGCTTTGAAACTTCAAG+TGG	0.597044	5.2:+59956241	MS.gene000729:CDS
AATGCAAAAGAGAAGCAAAA+TGG	0.391258	5.2:+59956286	MS.gene000729:CDS
ACAAACTTGTGCATGGTGCA+AGG	0.588546	5.2:+59956214	MS.gene000729:CDS
ACAATGTAGAATGTTGTTGT+TGG	0.495112	5.2:-59955849	None:intergenic
ACACTTTGAGGATCCTTGGA+TGG	0.541193	5.2:-59956795	None:intergenic
ACATAAGTCAAATCCATCCA+AGG	0.604373	5.2:+59956782	MS.gene000729:CDS
ACCTTGGCAGCAACACTTTG+AGG	0.436246	5.2:-59956807	None:intergenic
ACTAATTGCTTTCTCCAACA+TGG	0.596287	5.2:-59957122	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATGCAAAAGAGAAGCAAAA+TGG	+	chr5.2:59956286-59956305	MS.gene000729:CDS	30.0%
AATTTACAAAGACTTTGGCT+TGG	-	chr5.2:59957044-59957063	None:intergenic	30.0%
ACAATGTAGAATGTTGTTGT+TGG	-	chr5.2:59955852-59955871	None:intergenic	30.0%
GAGTACTTCATGAAATTGAA+TGG	+	chr5.2:59956669-59956688	MS.gene000729:intron	30.0%
TAGAGTAGGAGAATTTGTAA+TGG	-	chr5.2:59955996-59956015	None:intergenic	30.0%
TATTACAACAATCTCATGCA+AGG	+	chr5.2:59956100-59956119	MS.gene000729:CDS	30.0%
TCTATGATTGAAGAAGAAGA+TGG	+	chr5.2:59955923-59955942	MS.gene000729:CDS	30.0%
TGATAGCTTAATAAAAGAGG+CGG	+	chr5.2:59956372-59956391	MS.gene000729:CDS	30.0%
AAGTAACACAAACTTGTGCA+TGG	+	chr5.2:59956207-59956226	MS.gene000729:CDS	35.0%
ACATAAGTCAAATCCATCCA+AGG	+	chr5.2:59956782-59956801	MS.gene000729:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	59955839	59957436	59955839	ID=MS.gene000729
chr5.2	mRNA	59955839	59957436	59955839	ID=MS.gene000729.t1;Parent=MS.gene000729
chr5.2	exon	59955839	59956432	59955839	ID=MS.gene000729.t1.exon1;Parent=MS.gene000729.t1
chr5.2	CDS	59955839	59956432	59955839	ID=cds.MS.gene000729.t1;Parent=MS.gene000729.t1
chr5.2	exon	59956720	59956827	59956720	ID=MS.gene000729.t1.exon2;Parent=MS.gene000729.t1
chr5.2	CDS	59956720	59956827	59956720	ID=cds.MS.gene000729.t1;Parent=MS.gene000729.t1
chr5.2	exon	59956921	59956986	59956921	ID=MS.gene000729.t1.exon3;Parent=MS.gene000729.t1
chr5.2	CDS	59956921	59956986	59956921	ID=cds.MS.gene000729.t1;Parent=MS.gene000729.t1
chr5.2	exon	59957109	59957174	59957109	ID=MS.gene000729.t1.exon4;Parent=MS.gene000729.t1
chr5.2	CDS	59957109	59957174	59957109	ID=cds.MS.gene000729.t1;Parent=MS.gene000729.t1
chr5.2	exon	59957335	59957436	59957335	ID=MS.gene000729.t1.exon5;Parent=MS.gene000729.t1
chr5.2	CDS	59957335	59957436	59957335	ID=cds.MS.gene000729.t1;Parent=MS.gene000729.t1

Gene Sequence

>MS.gene000729

ATGGCACTAGCCAACAACAACATTCTACATTGTATTTTTGACGAGACTACTATGGAGATTAACTCTGTAATGTCTCATAAAAAATCTATGATTGAAGAAGAAGATGGTTCACAAAGCACTAATGGATTAAGCAATGATTCAGCTTCAGCTTCTGCCATTACAAATTCTCCTACTCTATGCAGAAAAGGGCATGTTTATAACAACAATACAAGTTATGAACTTGAAGAAGAGTCTTTGATTAATTTCAAAGGTAATGATGAATATTACAACAATCTCATGCAAGGTAGTGAATCATTGCTTAGCTTTCAGCAGAGTTGGATGGTTTCCAATGAGAATAATTTGCAACAATGGAATCATGCTAGTCCTAAAAGTAACACAAACTTGTGCATGGTGCAAGGTTTCAATAGCTTTGAAACTTCAAGTGGTAGCTATGGATCCATATTCAATAATGCAAAAGAGAAGCAAAATGGTGAAAGTTCATGTGGATGGCTATACTCTGAACCAAATGTCCCTTGTGATGACGACGACGATGATGATAGCTTAATAAAAGAGGCGGAAACACATGAATCCGTCTTGAAGAAGCGATCTTCTATGGTATTGCATCTTTAGTAGTTAAGCAAAATCTTTTACCATTTATGATGTTTAGAGTCATTGTTTTATATCAGCTTGAAGTGCTTGTGTCTCAATCCTTGATATATTGCACATAACCGCGGTCAAATGGGTCACAGAGACATCAAGAATCTTGATGTTTCTGTCCTGATCGCGGTTGCAGACCGTTAATTTTATTAATTTTTTTCAAATTATTAGAACATATTTAGGACTAGTTTTTTGAGTACTTCATGAAATTGAATGGAAAATTGAGATAAAATTTGTGTTTATAGGGGGGAAACATGCAACCTACCAATGCCAAGAAGCCATGCACAGCTGCAAGTAAGAAAGAAAAACATAAGTCAAATCCATCCAAGGATCCTCAAAGTGTTGCTGCCAAGGTTTGCTTCAAACCCAAGTTATATCATGTTTTGATTGAAATTCAAGCAAACTTAAAATATTTAGTGTTGATAGAATGTTTGTTTGTATCACAGAATAGAAGAGAGAGAATAAGTGAGAGGCTCAAGATCCTACAAGAGCTTGTGCCAAACGGTTCCAAGGTAGATTTTCAAATTAGTCTTCAAAGTTATGTTGCTTTCTTTACATTTCTTTATCCAAGCCAAAGTCTTTGTAAATTTTTCCCAATTAGAATTATATTTAATTCTGATGATGAATTTTGCAGGTTGATTTGGTTACCATGTTGGAGAAAGCAATTAGTTATGTGAAGTTTCTTCAGTTGCAAGTGAAGGTGAGACTTTAGCACAAATACTCAATTTCTTTAAAAATATGAGAGAAAATGAAAGTTTAGGGACTACTTTGATAAATTAAAAATTGTTTAGGGACTAGTTATTATATTTTTCTTGGTTTTGATTTGTGACATTTTTGTTATATATTTGTTCATGGTGTAGGTGTTGGCAGCTGATGAATTTTGGCCAGTCAATGGTGGAAAAGCTCCTGACATTGGTCAAGTAAAACAAGCATTTGATGCAATTCTTTCATCTCAAAGATGA

Protein sequence

>MS.gene000729.t1

MALANNNILHCIFDETTMEINSVMSHKKSMIEEEDGSQSTNGLSNDSASASAITNSPTLCRKGHVYNNNTSYELEEESLINFKGNDEYYNNLMQGSESLLSFQQSWMVSNENNLQQWNHASPKSNTNLCMVQGFNSFETSSGSYGSIFNNAKEKQNGESSCGWLYSEPNVPCDDDDDDDSLIKEAETHESVLKKRSSMGGNMQPTNAKKPCTAASKKEKHKSNPSKDPQSVAAKNRRERISERLKILQELVPNGSKVDLVTMLEKAISYVKFLQLQVKVLAADEFWPVNGGKAPDIGQVKQAFDAILSSQR