Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000912.t1 | RHN65210.1 | 66.2 | 154 | 27 | 5 | 3 | 131 | 312 | 465 | 3.70E-39 | 171 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000912.t1 | A0A072UTW5 | 66.2 | 154 | 27 | 5 | 3 | 131 | 318 | 471 | 2.7e-39 | 171.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene000912.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene000912.t1 | MTR_3g005495 | 65.385 | 156 | 29 | 5 | 1 | 131 | 316 | 471 | 2.63e-52 | 172 |
| MS.gene000912.t1 | MTR_3g005495 | 40.441 | 136 | 71 | 3 | 4 | 129 | 89 | 224 | 9.22e-21 | 87.0 |
| MS.gene000912.t1 | MTR_3g005420 | 38.824 | 170 | 64 | 4 | 2 | 131 | 363 | 532 | 3.82e-23 | 94.0 |
| MS.gene000912.t1 | MTR_3g005420 | 39.583 | 144 | 69 | 5 | 4 | 129 | 117 | 260 | 3.87e-18 | 79.7 |
| MS.gene000912.t1 | MTR_3g005490 | 39.623 | 159 | 65 | 5 | 4 | 131 | 97 | 255 | 1.55e-21 | 87.0 |
| MS.gene000912.t1 | MTR_5g070440 | 37.870 | 169 | 65 | 5 | 3 | 131 | 96 | 264 | 4.04e-21 | 86.3 |
| MS.gene000912.t1 | MTR_3g005480 | 36.242 | 149 | 64 | 3 | 14 | 131 | 108 | 256 | 5.38e-21 | 85.5 |
| MS.gene000912.t1 | MTR_3g005420 | 39.583 | 144 | 69 | 5 | 4 | 129 | 98 | 241 | 3.18e-18 | 79.7 |
| MS.gene000912.t1 | MTR_3g005420 | 44.860 | 107 | 56 | 2 | 28 | 131 | 372 | 478 | 1.00e-17 | 78.6 |
| MS.gene000912.t1 | MTR_3g005460 | 36.364 | 143 | 74 | 4 | 4 | 129 | 101 | 243 | 1.43e-15 | 72.4 |
| MS.gene000912.t1 | MTR_3g005460 | 31.638 | 177 | 74 | 5 | 2 | 131 | 337 | 513 | 5.47e-14 | 67.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGCTTTCCATTGCAATTT+TGG | 0.107151 | 7.1:+40744892 | None:intergenic |
| TGTTGTCCGCTGCAAATCTA+AGG | 0.346545 | 7.1:+40744814 | None:intergenic |
| TTGAAAACTCCAGGTACTAA+TGG | 0.380907 | 7.1:-40745135 | MS.gene000912:CDS |
| TATTATATTTGATGTAACTT+TGG | 0.382106 | 7.1:-40745175 | MS.gene000912:CDS |
| TGTACACGTTCAGGTTGTTC+AGG | 0.384006 | 7.1:-40744858 | MS.gene000912:CDS |
| GATGTTACTTGTACACGTTC+AGG | 0.395352 | 7.1:-40744867 | MS.gene000912:CDS |
| GGTTGTTCAGGACTGCAATT+GGG | 0.399264 | 7.1:-40744846 | MS.gene000912:CDS |
| AGGTTGTTCAGGACTGCAAT+TGG | 0.448259 | 7.1:-40744847 | MS.gene000912:CDS |
| CGACGAAGTTGTAACACAAC+TGG | 0.478088 | 7.1:+40744744 | None:intergenic |
| TGCAGCGGACAACAATTTGT+CGG | 0.480786 | 7.1:-40744805 | MS.gene000912:CDS |
| TACTATCCATTGAAAACTCC+AGG | 0.499075 | 7.1:-40745144 | MS.gene000912:CDS |
| TATAGGTCGAAGTAAAAGAA+AGG | 0.499429 | 7.1:+40745079 | None:intergenic |
| AAATTGCAATGGAAAGCAAT+GGG | 0.520291 | 7.1:-40744889 | MS.gene000912:CDS |
| AAAATTGCAATGGAAAGCAA+TGG | 0.527431 | 7.1:-40744890 | MS.gene000912:CDS |
| ATTGTTTCTCCATTAGTACC+TGG | 0.540138 | 7.1:+40745126 | None:intergenic |
| GACCTATAAGTATAAAACTC+TGG | 0.543958 | 7.1:-40745064 | MS.gene000912:intron |
| TCAGGACTGCAATTGGGGAA+AGG | 0.545358 | 7.1:-40744840 | MS.gene000912:CDS |
| GAAACAATTAGTGAGGATGA+AGG | 0.546377 | 7.1:-40745111 | MS.gene000912:CDS |
| GTCGGACGGTGATAAATACA+AGG | 0.575596 | 7.1:-40744787 | MS.gene000912:CDS |
| GTTGTTCAGGACTGCAATTG+GGG | 0.594825 | 7.1:-40744845 | MS.gene000912:CDS |
| AGGATTCCTTAGATTTGCAG+CGG | 0.644386 | 7.1:-40744820 | MS.gene000912:CDS |
| GTGAAGCTCCAAAATTGCAA+TGG | 0.646086 | 7.1:-40744900 | MS.gene000912:CDS |
| GCGGACAACAATTTGTCGGA+CGG | 0.655494 | 7.1:-40744801 | MS.gene000912:CDS |
| TAATGGAGAAACAATTAGTG+AGG | 0.660327 | 7.1:-40745118 | MS.gene000912:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTATATTTGATGTAACTT+TGG | - | chr7.1:40744775-40744794 | MS.gene000912:CDS | 15.0% |
| !!! | ATGTTTACTTTTGCAACAAA+TGG | - | chr7.1:40744737-40744756 | MS.gene000912:CDS | 25.0% |
| AAAATTGCAATGGAAAGCAA+TGG | - | chr7.1:40745060-40745079 | MS.gene000912:intron | 30.0% | |
| AAATTGCAATGGAAAGCAAT+GGG | - | chr7.1:40745061-40745080 | MS.gene000912:intron | 30.0% | |
| GACCTATAAGTATAAAACTC+TGG | - | chr7.1:40744886-40744905 | MS.gene000912:CDS | 30.0% | |
| TAATGGAGAAACAATTAGTG+AGG | - | chr7.1:40744832-40744851 | MS.gene000912:CDS | 30.0% | |
| TATAGGTCGAAGTAAAAGAA+AGG | + | chr7.1:40744874-40744893 | None:intergenic | 30.0% | |
| ! | ATTGCTTTCCATTGCAATTT+TGG | + | chr7.1:40745061-40745080 | None:intergenic | 30.0% |
| !! | CACCAGAGTTTTATACTTAT+AGG | + | chr7.1:40744891-40744910 | None:intergenic | 30.0% |
| AGCATTAATATACTGCACAG+GGG | + | chr7.1:40744994-40745013 | None:intergenic | 35.0% | |
| AGCGAGGACACATTAATTTA+AGG | + | chr7.1:40744971-40744990 | None:intergenic | 35.0% | |
| CAGCATTAATATACTGCACA+GGG | + | chr7.1:40744995-40745014 | None:intergenic | 35.0% | |
| GAAACAATTAGTGAGGATGA+AGG | - | chr7.1:40744839-40744858 | MS.gene000912:CDS | 35.0% | |
| TACTATCCATTGAAAACTCC+AGG | - | chr7.1:40744806-40744825 | MS.gene000912:CDS | 35.0% | |
| TCAGCATTAATATACTGCAC+AGG | + | chr7.1:40744996-40745015 | None:intergenic | 35.0% | |
| TTGAAAACTCCAGGTACTAA+TGG | - | chr7.1:40744815-40744834 | MS.gene000912:CDS | 35.0% | |
| ! | ATTGTTTCTCCATTAGTACC+TGG | + | chr7.1:40744827-40744846 | None:intergenic | 35.0% |
| ! | TTAGTACCTGGAGTTTTCAA+TGG | + | chr7.1:40744815-40744834 | None:intergenic | 35.0% |
| AGGATTCCTTAGATTTGCAG+CGG | - | chr7.1:40745130-40745149 | MS.gene000912:CDS | 40.0% | |
| GTATAAAACTCTGGTGAGTG+AGG | - | chr7.1:40744895-40744914 | MS.gene000912:CDS | 40.0% | |
| GTGAAGCTCCAAAATTGCAA+TGG | - | chr7.1:40745050-40745069 | MS.gene000912:intron | 40.0% | |
| ! | GATGTTACTTGTACACGTTC+AGG | - | chr7.1:40745083-40745102 | MS.gene000912:CDS | 40.0% |
| AGGTTGTTCAGGACTGCAAT+TGG | - | chr7.1:40745103-40745122 | MS.gene000912:CDS | 45.0% | |
| CGACGAAGTTGTAACACAAC+TGG | + | chr7.1:40745209-40745228 | None:intergenic | 45.0% | |
| GGTTGTTCAGGACTGCAATT+GGG | - | chr7.1:40745104-40745123 | MS.gene000912:CDS | 45.0% | |
| GTCGGACGGTGATAAATACA+AGG | - | chr7.1:40745163-40745182 | MS.gene000912:CDS | 45.0% | |
| GTTGTTCAGGACTGCAATTG+GGG | - | chr7.1:40745105-40745124 | MS.gene000912:CDS | 45.0% | |
| TGCAGCGGACAACAATTTGT+CGG | - | chr7.1:40745145-40745164 | MS.gene000912:CDS | 45.0% | |
| TGTTGTCCGCTGCAAATCTA+AGG | + | chr7.1:40745139-40745158 | None:intergenic | 45.0% | |
| !! | TGTACACGTTCAGGTTGTTC+AGG | - | chr7.1:40745092-40745111 | MS.gene000912:CDS | 45.0% |
| GCGGACAACAATTTGTCGGA+CGG | - | chr7.1:40745149-40745168 | MS.gene000912:CDS | 50.0% | |
| TCAGGACTGCAATTGGGGAA+AGG | - | chr7.1:40745110-40745129 | MS.gene000912:CDS | 50.0% | |
| ATATACTGCACAGGGGAGCG+AGG | + | chr7.1:40744987-40745006 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 40744737 | 40745235 | 40744737 | ID=MS.gene000912 |
| chr7.1 | mRNA | 40744737 | 40745235 | 40744737 | ID=MS.gene000912.t1;Parent=MS.gene000912 |
| chr7.1 | exon | 40745065 | 40745235 | 40745065 | ID=MS.gene000912.t1.exon1;Parent=MS.gene000912.t1 |
| chr7.1 | CDS | 40745065 | 40745235 | 40745065 | ID=cds.MS.gene000912.t1;Parent=MS.gene000912.t1 |
| chr7.1 | exon | 40744737 | 40744970 | 40744737 | ID=MS.gene000912.t1.exon2;Parent=MS.gene000912.t1 |
| chr7.1 | CDS | 40744737 | 40744970 | 40744737 | ID=cds.MS.gene000912.t1;Parent=MS.gene000912.t1 |
| Gene Sequence |
| Protein sequence |
