Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001058.t1 | XP_024639602.1 | 97.2 | 145 | 3 | 1 | 1 | 145 | 64 | 207 | 3.40E-70 | 274.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001058.t1 | Q9SZL8 | 89.7 | 145 | 13 | 2 | 1 | 145 | 77 | 219 | 3.0e-67 | 255.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001058.t1 | A0A396HY09 | 97.2 | 145 | 3 | 1 | 1 | 145 | 64 | 207 | 2.5e-70 | 274.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene001058.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001058.t1 | MTR_3g006370 | 50.962 | 104 | 47 | 3 | 1 | 104 | 54 | 153 | 1.30e-25 | 97.4 |
| MS.gene001058.t1 | MTR_4g067290 | 51.923 | 104 | 44 | 4 | 2 | 105 | 61 | 158 | 2.05e-24 | 94.4 |
| MS.gene001058.t1 | MTR_4g067290 | 51.923 | 104 | 44 | 4 | 2 | 105 | 61 | 158 | 2.11e-24 | 94.4 |
| MS.gene001058.t1 | MTR_4g067290 | 51.923 | 104 | 44 | 4 | 2 | 105 | 81 | 178 | 2.91e-24 | 94.7 |
| MS.gene001058.t1 | MTR_4g067290 | 51.923 | 104 | 44 | 4 | 2 | 105 | 79 | 176 | 2.94e-24 | 94.4 |
| MS.gene001058.t1 | MTR_4g067290 | 51.923 | 104 | 44 | 4 | 2 | 105 | 81 | 178 | 3.09e-24 | 94.4 |
| MS.gene001058.t1 | MTR_4g067290 | 51.923 | 104 | 44 | 4 | 2 | 105 | 121 | 218 | 6.05e-24 | 94.4 |
| MS.gene001058.t1 | MTR_2g098920 | 52.885 | 104 | 43 | 4 | 2 | 105 | 85 | 182 | 1.35e-23 | 92.8 |
| MS.gene001058.t1 | MTR_0402s0040 | 37.500 | 136 | 80 | 3 | 1 | 136 | 30 | 160 | 3.15e-23 | 94.7 |
| MS.gene001058.t1 | MTR_2g098880 | 42.202 | 109 | 60 | 3 | 1 | 109 | 65 | 170 | 7.98e-23 | 90.9 |
| MS.gene001058.t1 | MTR_2g098880 | 42.202 | 109 | 60 | 3 | 1 | 109 | 77 | 182 | 1.06e-22 | 90.5 |
| MS.gene001058.t1 | MTR_1g070355 | 33.562 | 146 | 94 | 3 | 2 | 147 | 79 | 221 | 2.99e-19 | 83.6 |
| MS.gene001058.t1 | MTR_5g015480 | 36.913 | 149 | 82 | 5 | 1 | 141 | 60 | 204 | 3.59e-19 | 81.3 |
| MS.gene001058.t1 | MTR_7g028855 | 34.286 | 140 | 89 | 2 | 1 | 140 | 124 | 260 | 8.15e-17 | 76.3 |
| MS.gene001058.t1 | MTR_2g070590 | 36.893 | 103 | 61 | 2 | 1 | 103 | 21 | 119 | 1.69e-16 | 71.2 |
| MS.gene001058.t1 | MTR_1g019850 | 32.090 | 134 | 80 | 3 | 1 | 134 | 74 | 196 | 1.12e-15 | 71.6 |
| MS.gene001058.t1 | MTR_2g101460 | 38.333 | 120 | 67 | 3 | 3 | 120 | 61 | 175 | 1.22e-15 | 70.9 |
| MS.gene001058.t1 | MTR_2g101460 | 38.655 | 119 | 66 | 3 | 1 | 117 | 49 | 162 | 1.36e-15 | 70.5 |
| MS.gene001058.t1 | MTR_0853s0010 | 29.710 | 138 | 85 | 3 | 1 | 134 | 29 | 158 | 1.74e-14 | 67.0 |
| MS.gene001058.t1 | MTR_7g446520 | 35.417 | 144 | 72 | 4 | 1 | 144 | 92 | 214 | 2.48e-14 | 68.9 |
| MS.gene001058.t1 | MTR_7g113010 | 35.593 | 118 | 58 | 3 | 1 | 118 | 461 | 560 | 3.28e-14 | 68.9 |
| MS.gene001058.t1 | MTR_4g035820 | 31.724 | 145 | 90 | 3 | 3 | 143 | 81 | 220 | 3.73e-14 | 68.9 |
| MS.gene001058.t1 | MTR_6g017200 | 35.417 | 96 | 58 | 2 | 1 | 96 | 62 | 153 | 1.66e-13 | 64.3 |
| MS.gene001058.t1 | MTR_5g076070 | 28.030 | 132 | 92 | 2 | 3 | 134 | 59 | 187 | 4.56e-13 | 65.1 |
| MS.gene001058.t1 | MTR_1g008290 | 35.802 | 81 | 47 | 2 | 65 | 145 | 15 | 90 | 1.96e-12 | 61.2 |
| MS.gene001058.t1 | MTR_6g051425 | 26.429 | 140 | 102 | 1 | 4 | 143 | 24 | 162 | 3.60e-12 | 62.8 |
| MS.gene001058.t1 | MTR_6g022080 | 27.273 | 143 | 99 | 3 | 3 | 144 | 91 | 229 | 4.33e-12 | 62.0 |
| MS.gene001058.t1 | MTR_1g057920 | 36.667 | 90 | 53 | 2 | 1 | 90 | 38 | 123 | 2.19e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001058.t1 | AT4G38180 | 87.586 | 145 | 16 | 2 | 1 | 145 | 77 | 219 | 1.14e-77 | 245 |
| MS.gene001058.t1 | AT3G07500 | 52.381 | 105 | 45 | 3 | 1 | 105 | 37 | 136 | 1.85e-28 | 104 |
| MS.gene001058.t1 | AT3G59470 | 53.846 | 104 | 42 | 4 | 2 | 105 | 75 | 172 | 1.86e-27 | 102 |
| MS.gene001058.t1 | AT3G59470 | 53.846 | 104 | 42 | 4 | 2 | 105 | 75 | 172 | 1.86e-27 | 102 |
| MS.gene001058.t1 | AT3G59470 | 53.846 | 104 | 42 | 4 | 2 | 105 | 87 | 184 | 2.54e-27 | 102 |
| MS.gene001058.t1 | AT2G43280 | 49.107 | 112 | 51 | 4 | 3 | 112 | 28 | 135 | 4.96e-25 | 95.5 |
| MS.gene001058.t1 | AT1G76320 | 43.966 | 116 | 56 | 5 | 1 | 116 | 1 | 107 | 1.54e-21 | 90.1 |
| MS.gene001058.t1 | AT1G76320 | 43.966 | 116 | 56 | 5 | 1 | 116 | 1 | 107 | 1.56e-21 | 90.1 |
| MS.gene001058.t1 | AT1G76320 | 43.966 | 116 | 56 | 5 | 1 | 116 | 1 | 107 | 1.56e-21 | 90.1 |
| MS.gene001058.t1 | AT1G76320 | 43.966 | 116 | 56 | 5 | 1 | 116 | 1 | 107 | 2.12e-21 | 89.7 |
| MS.gene001058.t1 | AT2G32250 | 41.322 | 121 | 63 | 4 | 1 | 121 | 42 | 154 | 1.32e-20 | 87.4 |
| MS.gene001058.t1 | AT2G32250 | 41.322 | 121 | 63 | 4 | 1 | 121 | 1 | 113 | 1.36e-20 | 87.4 |
| MS.gene001058.t1 | AT2G32250 | 41.322 | 121 | 63 | 4 | 1 | 121 | 1 | 113 | 1.37e-20 | 87.4 |
| MS.gene001058.t1 | AT2G32250 | 41.322 | 121 | 63 | 4 | 1 | 121 | 42 | 154 | 1.47e-20 | 87.4 |
| MS.gene001058.t1 | AT2G32250 | 41.322 | 121 | 63 | 4 | 1 | 121 | 42 | 154 | 1.47e-20 | 87.4 |
| MS.gene001058.t1 | AT2G32250 | 41.322 | 121 | 63 | 4 | 1 | 121 | 42 | 154 | 1.56e-20 | 87.0 |
| MS.gene001058.t1 | AT2G32250 | 41.322 | 121 | 63 | 4 | 1 | 121 | 42 | 154 | 1.56e-20 | 87.0 |
| MS.gene001058.t1 | AT2G32250 | 41.322 | 121 | 63 | 4 | 1 | 121 | 42 | 154 | 1.56e-20 | 87.0 |
| MS.gene001058.t1 | AT2G43280 | 46.602 | 103 | 49 | 4 | 12 | 112 | 2 | 100 | 2.05e-20 | 82.4 |
| MS.gene001058.t1 | AT4G15090 | 35.606 | 132 | 79 | 4 | 1 | 132 | 55 | 180 | 1.77e-18 | 81.3 |
| MS.gene001058.t1 | AT4G15090 | 35.606 | 132 | 79 | 4 | 1 | 132 | 55 | 180 | 1.77e-18 | 81.3 |
| MS.gene001058.t1 | AT4G15090 | 35.606 | 132 | 79 | 4 | 1 | 132 | 55 | 180 | 1.77e-18 | 81.3 |
| MS.gene001058.t1 | AT4G15090 | 35.606 | 132 | 79 | 4 | 1 | 132 | 55 | 180 | 1.77e-18 | 81.3 |
| MS.gene001058.t1 | AT4G15090 | 35.606 | 132 | 79 | 4 | 1 | 132 | 55 | 180 | 1.77e-18 | 81.3 |
| MS.gene001058.t1 | AT3G22170 | 37.903 | 124 | 55 | 4 | 1 | 109 | 74 | 190 | 6.49e-18 | 79.7 |
| MS.gene001058.t1 | AT3G22170 | 37.903 | 124 | 55 | 4 | 1 | 109 | 74 | 190 | 6.49e-18 | 79.7 |
| MS.gene001058.t1 | AT4G12850 | 40.777 | 103 | 53 | 3 | 1 | 103 | 15 | 109 | 1.49e-17 | 74.3 |
| MS.gene001058.t1 | AT4G12850 | 40.777 | 103 | 53 | 3 | 1 | 103 | 15 | 109 | 2.90e-17 | 73.6 |
| MS.gene001058.t1 | AT4G12850 | 40.777 | 103 | 53 | 3 | 1 | 103 | 15 | 109 | 5.61e-17 | 73.9 |
| MS.gene001058.t1 | AT5G28530 | 36.806 | 144 | 83 | 5 | 3 | 143 | 61 | 199 | 6.19e-16 | 73.9 |
| MS.gene001058.t1 | AT5G28530 | 36.806 | 144 | 83 | 5 | 3 | 143 | 61 | 199 | 6.40e-16 | 73.9 |
| MS.gene001058.t1 | AT5G28530 | 36.806 | 144 | 83 | 5 | 3 | 143 | 61 | 199 | 6.40e-16 | 73.9 |
| MS.gene001058.t1 | AT5G28530 | 36.806 | 144 | 83 | 5 | 3 | 143 | 61 | 199 | 6.62e-16 | 73.9 |
| MS.gene001058.t1 | AT5G28530 | 36.806 | 144 | 83 | 5 | 3 | 143 | 61 | 199 | 6.86e-16 | 73.9 |
| MS.gene001058.t1 | AT1G10240 | 32.168 | 143 | 93 | 3 | 3 | 143 | 54 | 194 | 2.43e-15 | 72.4 |
| MS.gene001058.t1 | AT1G10240 | 32.168 | 143 | 93 | 3 | 3 | 143 | 54 | 194 | 2.43e-15 | 72.4 |
| MS.gene001058.t1 | AT5G18960 | 39.806 | 103 | 44 | 4 | 1 | 103 | 47 | 131 | 4.17e-15 | 71.6 |
| MS.gene001058.t1 | AT5G18960 | 39.806 | 103 | 44 | 4 | 1 | 103 | 47 | 131 | 4.17e-15 | 71.6 |
| MS.gene001058.t1 | AT3G06250 | 38.095 | 105 | 47 | 3 | 1 | 105 | 194 | 280 | 6.00e-15 | 71.2 |
| MS.gene001058.t1 | AT3G06250 | 38.835 | 103 | 45 | 4 | 1 | 103 | 32 | 116 | 1.32e-14 | 70.1 |
| MS.gene001058.t1 | AT3G06250 | 38.095 | 105 | 47 | 3 | 1 | 105 | 194 | 280 | 6.00e-15 | 71.2 |
| MS.gene001058.t1 | AT3G06250 | 38.835 | 103 | 45 | 4 | 1 | 103 | 32 | 116 | 1.32e-14 | 70.1 |
| MS.gene001058.t1 | AT3G06250 | 38.095 | 105 | 47 | 3 | 1 | 105 | 194 | 280 | 6.00e-15 | 71.2 |
| MS.gene001058.t1 | AT3G06250 | 38.835 | 103 | 45 | 4 | 1 | 103 | 32 | 116 | 1.32e-14 | 70.1 |
| MS.gene001058.t1 | AT2G27110 | 33.065 | 124 | 63 | 3 | 1 | 124 | 53 | 156 | 5.03e-14 | 68.6 |
| MS.gene001058.t1 | AT2G27110 | 33.065 | 124 | 63 | 3 | 1 | 124 | 53 | 156 | 5.03e-14 | 68.6 |
| MS.gene001058.t1 | AT4G19990 | 37.391 | 115 | 54 | 4 | 2 | 103 | 26 | 135 | 1.38e-13 | 67.4 |
| MS.gene001058.t1 | AT4G19990 | 37.391 | 115 | 54 | 4 | 2 | 103 | 26 | 135 | 1.39e-13 | 67.4 |
| MS.gene001058.t1 | AT4G19990 | 37.391 | 115 | 54 | 4 | 2 | 103 | 26 | 135 | 1.39e-13 | 67.4 |
| MS.gene001058.t1 | AT4G19990 | 37.391 | 115 | 54 | 4 | 2 | 103 | 40 | 149 | 1.44e-13 | 67.4 |
| MS.gene001058.t1 | AT4G19990 | 37.391 | 115 | 54 | 4 | 2 | 103 | 26 | 135 | 1.54e-13 | 67.0 |
| MS.gene001058.t1 | AT4G19990 | 37.391 | 115 | 54 | 4 | 2 | 103 | 26 | 135 | 1.54e-13 | 67.0 |
Find 0 sgRNAs with CRISPR-Local
Find 34 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTCGTTTGTGCTAAGGAA+GGG | - | 16633:116670-116689 | MS.gene001058:CDS | 35.0% | |
| TTGCAGATCCAGAAATTTGA+CGG | + | 16633:116890-116909 | MS.gene001058:intergenic | 35.0% | |
| AACCCTAATTGACACTCTAC+AGG | - | 16633:116911-116930 | MS.gene001058:CDS | 40.0% | |
| AATTCCTATGCTCGTTGTGT+TGG | - | 16633:116582-116601 | MS.gene001058:CDS | 40.0% | |
| AGATCCAGAAATTTGACGGT+GGG | + | 16633:116886-116905 | MS.gene001058:intergenic | 40.0% | |
| AGTGCAGACATAATTCTGTG+AGG | + | 16633:116951-116970 | MS.gene001058:intergenic | 40.0% | |
| CAATTCGTTTGTGCTAAGGA+AGG | - | 16633:116669-116688 | MS.gene001058:CDS | 40.0% | |
| GAGACAATTCGTTTGTGCTA+AGG | - | 16633:116665-116684 | MS.gene001058:CDS | 40.0% | |
| ! | AGCCTGTAGAGTGTCAATTA+GGG | + | 16633:116916-116935 | MS.gene001058:intergenic | 40.0% |
| !! | GTGCATTTTGACAGATAACG+AGG | + | 16633:116775-116794 | MS.gene001058:intergenic | 40.0% |
| AAATTTGACGGTGGGTACGA+AGG | + | 16633:116878-116897 | MS.gene001057:intergenic | 45.0% | |
| CAGATCCAGAAATTTGACGG+TGG | + | 16633:116887-116906 | MS.gene001058:intergenic | 45.0% | |
| GAAACCAACACAACGAGCAT+AGG | + | 16633:116589-116608 | MS.gene001058:intergenic | 45.0% | |
| GCGTTTGATCTCTCTGTCTT+TGG | + | 16633:116721-116740 | MS.gene001058:intergenic | 45.0% | |
| ! | CAGCCTGTAGAGTGTCAATT+AGG | + | 16633:116917-116936 | MS.gene001058:intergenic | 45.0% |
| ACGAAGGCAGTGAACCTGAT+CGG | + | 16633:116862-116881 | MS.gene001058:intergenic | 50.0% | |
| ACTCTACAGGCTGCTGGTAT+GGG | - | 16633:116924-116943 | MS.gene001058:CDS | 50.0% | |
| ATTGACACTCTACAGGCTGC+TGG | - | 16633:116918-116937 | MS.gene001058:CDS | 50.0% | |
| CAACCCTAGTGACTGTTCGT+GGG | + | 16633:116743-116762 | MS.gene001058:intergenic | 50.0% | |
| CGTACCCACCGTCAAATTTC+TGG | - | 16633:116879-116898 | MS.gene001058:CDS | 50.0% | |
| GAAGCCAGATACGATCCACT+TGG | + | 16633:116808-116827 | MS.gene001058:intergenic | 50.0% | |
| TCTGAATGATAGCTCCGTCG+CGG | + | 16633:116647-116666 | MS.gene001058:intergenic | 50.0% | |
| TCTGCCAAGTGGATCGTATC+TGG | - | 16633:116801-116820 | MS.gene001058:CDS | 50.0% | |
| TGCACGATTCTTCTGCCAAG+TGG | - | 16633:116790-116809 | MS.gene001058:CDS | 50.0% | |
| AACGCCCACGAACAGTCACT+AGG | - | 16633:116736-116755 | MS.gene001058:CDS | 55.0% | |
| ACGCCCACGAACAGTCACTA+GGG | - | 16633:116737-116756 | MS.gene001058:CDS | 55.0% | |
| CACTCTACAGGCTGCTGGTA+TGG | - | 16633:116923-116942 | MS.gene001058:CDS | 55.0% | |
| CCAACCCTAGTGACTGTTCG+TGG | + | 16633:116744-116763 | MS.gene001058:intergenic | 55.0% | |
| CCACGAACAGTCACTAGGGT+TGG | - | 16633:116741-116760 | MS.gene001058:CDS | 55.0% | |
| CGAAGGCAGTGAACCTGATC+GGG | + | 16633:116861-116880 | MS.gene001058:intergenic | 55.0% | |
| CGAGTCTGAAGAGGCTGCTA+AGG | - | 16633:116551-116570 | MS.gene001058:CDS | 55.0% | |
| ! | CGAACTTGTTCCACCCGATC+AGG | - | 16633:116845-116864 | MS.gene001058:CDS | 55.0% |
| AGGCAGTGAACCTGATCGGG+TGG | + | 16633:116858-116877 | MS.gene001058:intergenic | 60.0% | |
| TCTCGTCGTTCTCGCCGCGA+CGG | - | 16633:116630-116649 | MS.gene001058:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 16633 | gene | 116543 | 116989 | 116543 | ID=MS.gene001058 |
| 16633 | mRNA | 116543 | 116989 | 116543 | ID=MS.gene001058.t1;Parent=MS.gene001058 |
| 16633 | exon | 116543 | 116989 | 116543 | ID=MS.gene001058.t1.exon1;Parent=MS.gene001058.t1 |
| 16633 | CDS | 116543 | 116989 | 116543 | ID=cds.MS.gene001058.t1;Parent=MS.gene001058.t1 |
| Gene Sequence |
| Protein sequence |
