Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001150.t1 | XP_003613728.2 | 82.2 | 320 | 30 | 2 | 1 | 320 | 54 | 346 | 3.80E-114 | 421.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001150.t1 | Q8RWD7 | 41.8 | 79 | 44 | 1 | 1 | 79 | 95 | 171 | 5.7e-10 | 66.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001150.t1 | G7KDV8 | 82.2 | 320 | 30 | 2 | 1 | 320 | 54 | 346 | 2.7e-114 | 421.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene001150.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001150.t1 | MTR_7g039520 | 81.875 | 320 | 31 | 2 | 1 | 320 | 54 | 346 | 0.0 | 521 |
| MS.gene001150.t1 | MTR_5g040080 | 82.188 | 320 | 30 | 2 | 1 | 320 | 54 | 346 | 0.0 | 504 |
| MS.gene001150.t1 | MTR_2g014410 | 71.014 | 276 | 38 | 3 | 43 | 318 | 42 | 275 | 6.83e-125 | 361 |
| MS.gene001150.t1 | MTR_1g069015 | 30.357 | 112 | 71 | 2 | 1 | 111 | 59 | 164 | 4.40e-12 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001150.t1 | AT1G58210 | 43.038 | 79 | 43 | 1 | 1 | 79 | 95 | 171 | 3.08e-14 | 72.0 |
Find 51 sgRNAs with CRISPR-Local
Find 96 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTATAAATCACGCCAATATT+TGG | 0.228451 | 5.3:-28128691 | None:intergenic |
| ATCCTCTACAATCTTCTTCT+TGG | 0.240672 | 5.3:-28129091 | None:intergenic |
| ATCCTCTACAATCTTCTTCT+TGG | 0.240672 | 5.3:-28129124 | None:intergenic |
| ATCCTCTACAATCTTCTTCT+TGG | 0.277613 | 5.3:-28129157 | None:intergenic |
| GTGATTTATAATGGTGAAAA+TGG | 0.304153 | 5.3:+28128703 | MS.gene001150:CDS |
| GTGATTTCTAATGGTGAAAA+TGG | 0.304848 | 5.3:+28128913 | MS.gene001150:CDS |
| CAGTTGGATTTCCACACAAT+TGG | 0.315477 | 5.3:+28128509 | MS.gene001150:CDS |
| TTCAATTTCTTTGCATAAAG+TGG | 0.317065 | 5.3:-28129027 | None:intergenic |
| AATATTGGCGTGATTTATAA+TGG | 0.332979 | 5.3:+28128694 | MS.gene001150:CDS |
| AGCTTGTCTCAACCAAATAT+TGG | 0.339420 | 5.3:+28128679 | MS.gene001150:CDS |
| AAGTCACACGATGCTTAAAA+AGG | 0.345125 | 5.3:+28129230 | MS.gene001150:CDS |
| GCCTCACGTACATCTCAAAA+TGG | 0.367820 | 5.3:+28127562 | MS.gene001150:CDS |
| AGTGGAGATCTAACACTATC+TGG | 0.369178 | 5.3:-28129009 | None:intergenic |
| AATTATTCAGCTCATAGTTC+AGG | 0.376079 | 5.3:+28128535 | MS.gene001150:CDS |
| TGAAACTTTGCAGGAAGATA+TGG | 0.390389 | 5.3:+28128777 | MS.gene001150:CDS |
| GAAAACGTGGATAAAGTTAC+TGG | 0.399696 | 5.3:+28128568 | MS.gene001150:CDS |
| AGCAGCCGATGAAACTTTGC+AGG | 0.410172 | 5.3:+28128768 | MS.gene001150:CDS |
| CTGGTAACTTGTGTAGTAGT+AGG | 0.423638 | 5.3:+28129181 | MS.gene001150:CDS |
| GAAGGTAATGATAACATTGC+GGG | 0.433461 | 5.3:+28128805 | MS.gene001150:CDS |
| AAGAAGATTGTAGAGGATAC+TGG | 0.442437 | 5.3:+28129162 | MS.gene001150:CDS |
| CAGGTTGACGTGATTTCTAA+TGG | 0.445576 | 5.3:+28128904 | MS.gene001150:CDS |
| ATCTTGAGTCTCTACAACAT+TGG | 0.448912 | 5.3:-28127506 | None:intergenic |
| GCAATGTTATCATTACCTTC+AGG | 0.456016 | 5.3:-28128802 | None:intergenic |
| TCTTGAGTCTCTACAACATT+GGG | 0.460912 | 5.3:-28127505 | None:intergenic |
| AGCCTGGTAGATCTTCTAAC+AGG | 0.463228 | 5.3:-28129285 | None:intergenic |
| GTTGTCTCAACATGAAGATA+TGG | 0.473214 | 5.3:+28128834 | MS.gene001150:CDS |
| TTTCTTATAATTTAGTAGCC+TGG | 0.480534 | 5.3:-28129301 | None:intergenic |
| TCAACATGAAGATATGGATC+TGG | 0.495560 | 5.3:+28128840 | MS.gene001150:CDS |
| GTATGTCGCCGTTTCTCAGT+TGG | 0.506514 | 5.3:+28128493 | MS.gene001150:CDS |
| ATCTAACACTATCTGGCTCA+TGG | 0.508763 | 5.3:-28129002 | None:intergenic |
| ATCTTCCTGCAAAGTTTCAT+CGG | 0.511056 | 5.3:-28128773 | None:intergenic |
| TGGTAACTTGTGTAGTAGTA+GGG | 0.548652 | 5.3:+28129182 | MS.gene001150:CDS |
| GTGGAAATCCAACTGAGAAA+CGG | 0.556021 | 5.3:-28128501 | None:intergenic |
| TCTAACACTATCTGGCTCAT+GGG | 0.568783 | 5.3:-28129001 | None:intergenic |
| GGCATCACTGTAATGTTCCA+CGG | 0.577101 | 5.3:+28127529 | MS.gene001150:CDS |
| AACAGTTGACGAGACTTCAC+AGG | 0.579898 | 5.3:+28128624 | MS.gene001150:CDS |
| CTCCTGTTAGAAGATCTACC+AGG | 0.582686 | 5.3:+28129283 | MS.gene001150:CDS |
| CGAATTATCAAGTGACCGCA+AGG | 0.593011 | 5.3:+28129056 | MS.gene001150:CDS |
| ACACTATCTGGCTCATGGGT+AGG | 0.595465 | 5.3:-28128997 | None:intergenic |
| TGGTACGTTTCTTATCCTTG+CGG | 0.610799 | 5.3:-28129071 | None:intergenic |
| CAGGAAGATATGGAACCTGA+AGG | 0.622434 | 5.3:+28128787 | MS.gene001150:CDS |
| CATGAAGATATGGATCTGGA+AGG | 0.628269 | 5.3:+28128844 | MS.gene001150:CDS |
| AGGAAATGAATGTGAAAACG+TGG | 0.652661 | 5.3:+28128555 | MS.gene001150:CDS |
| GAGACTTCACAGGAAGCTGA+AGG | 0.656001 | 5.3:+28128634 | MS.gene001150:CDS |
| AATGTTGTAGAGACTCAAGA+TGG | 0.656225 | 5.3:+28127508 | MS.gene001150:CDS |
| TGAAGGTAATGATAACATTG+CGG | 0.667655 | 5.3:+28128804 | MS.gene001150:CDS |
| TACCAAGAAGAAGATTGTAG+AGG | 0.672640 | 5.3:+28129089 | MS.gene001150:CDS |
| TACCAAGAAGAAGATTGTAG+AGG | 0.672640 | 5.3:+28129122 | MS.gene001150:CDS |
| TACCAAGAAGAAGATTGTAG+AGG | 0.672640 | 5.3:+28129155 | MS.gene001150:CDS |
| TTAGAAATCACGTCAACCTG+TGG | 0.688603 | 5.3:-28128901 | None:intergenic |
| GTGAGGCGTTAATCAAACCG+TGG | 0.738580 | 5.3:-28127546 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAGTTAGCAAAAATAT+AGG | - | chr5.3:28128261-28128280 | None:intergenic | 15.0% |
| !!! | TCAATTTTTAGAATTAGTTT+TGG | + | chr5.3:28127969-28127988 | MS.gene001150:intron | 15.0% |
| !!! | TTAAAATCAAATTTTGTCAA+AGG | - | chr5.3:28127719-28127738 | None:intergenic | 15.0% |
| !! | TTGAGAGTAAAAATCAAATT+TGG | + | chr5.3:28127815-28127834 | MS.gene001150:intron | 20.0% |
| !! | TTGTGATAAAACAGTAAATT+TGG | - | chr5.3:28128091-28128110 | None:intergenic | 20.0% |
| !!! | AAGAATATTTTAAACATGTC+TGG | + | chr5.3:28127903-28127922 | MS.gene001150:intron | 20.0% |
| !!! | ATCAATCAATCACTTTTATT+TGG | + | chr5.3:28128222-28128241 | MS.gene001150:intron | 20.0% |
| !!! | TAACTTTTTTATGTGAAACT+AGG | + | chr5.3:28128271-28128290 | MS.gene001150:intron | 20.0% |
| !!! | TGTCAAAAAGTATAGATTTT+GGG | - | chr5.3:28128347-28128366 | None:intergenic | 20.0% |
| !!! | TTGTCAAAAAGTATAGATTT+TGG | - | chr5.3:28128348-28128367 | None:intergenic | 20.0% |
| ! | AAAATACGCATAATCTTCAA+TGG | + | chr5.3:28128389-28128408 | MS.gene001150:intron | 25.0% |
| ! | AAATTAAATGCGTCAATAAC+CGG | - | chr5.3:28128445-28128464 | None:intergenic | 25.0% |
| ! | AATATTCAATGTCTTTCAAC+AGG | + | chr5.3:28128471-28128490 | MS.gene001150:intron | 25.0% |
| ! | AATATTGGCGTGATTTATAA+TGG | + | chr5.3:28128694-28128713 | MS.gene001150:CDS | 25.0% |
| ! | GTGATTTATAATGGTGAAAA+TGG | + | chr5.3:28128703-28128722 | MS.gene001150:CDS | 25.0% |
| ! | TGATTGATAAACCTCAATTA+TGG | - | chr5.3:28128210-28128229 | None:intergenic | 25.0% |
| ! | TTATAAATCACGCCAATATT+TGG | - | chr5.3:28128694-28128713 | None:intergenic | 25.0% |
| ! | TTCAATTTCTTTGCATAAAG+TGG | - | chr5.3:28129030-28129049 | None:intergenic | 25.0% |
| ! | TTGAATATAACTAGTGTGTT+TGG | + | chr5.3:28127680-28127699 | MS.gene001150:intron | 25.0% |
| ! | TTTCTTATAATTTAGTAGCC+TGG | - | chr5.3:28129304-28129323 | None:intergenic | 25.0% |
| !! | GAATAATTTTTCCAATTGTG+TGG | - | chr5.3:28128523-28128542 | None:intergenic | 25.0% |
| !!! | TATTGACGCATTTAATTTTC+TGG | + | chr5.3:28128447-28128466 | MS.gene001150:intron | 25.0% |
| !!! | TTTAGATTCACTTTTAAGAG+AGG | - | chr5.3:28127941-28127960 | None:intergenic | 25.0% |
| AATTATTCAGCTCATAGTTC+AGG | + | chr5.3:28128535-28128554 | MS.gene001150:CDS | 30.0% | |
| GTGATTTCTAATGGTGAAAA+TGG | + | chr5.3:28128913-28128932 | MS.gene001150:CDS | 30.0% | |
| TGAAGGTAATGATAACATTG+CGG | + | chr5.3:28128804-28128823 | MS.gene001150:CDS | 30.0% | |
| ! | GTATGTTTGAAGCTATTACT+TGG | - | chr5.3:28128173-28128192 | None:intergenic | 30.0% |
| ! | TCAAAATGGTTTTTCTTCTG+AGG | + | chr5.3:28127576-28127595 | MS.gene001150:CDS | 30.0% |
| !! | CTTCGAGTTAGAATTCATTT+TGG | + | chr5.3:28127860-28127879 | MS.gene001150:intron | 30.0% |
| AAGAAGATTGTAGAGGATAC+TGG | + | chr5.3:28129162-28129181 | MS.gene001150:CDS | 35.0% | |
| AAGAGGAGTGAGAAAGAAAA+AGG | - | chr5.3:28127610-28127629 | None:intergenic | 35.0% | |
| AAGTCACACGATGCTTAAAA+AGG | + | chr5.3:28129230-28129249 | MS.gene001150:CDS | 35.0% | |
| AATGTTGTAGAGACTCAAGA+TGG | + | chr5.3:28127508-28127527 | MS.gene001150:CDS | 35.0% | |
| AGAGGAGTGAGAAAGAAAAA+GGG | - | chr5.3:28127609-28127628 | None:intergenic | 35.0% | |
| AGCTTGTCTCAACCAAATAT+TGG | + | chr5.3:28128679-28128698 | MS.gene001150:CDS | 35.0% | |
| AGGAAATGAATGTGAAAACG+TGG | + | chr5.3:28128555-28128574 | MS.gene001150:CDS | 35.0% | |
| ATCCTCTACAATCTTCTTCT+TGG | - | chr5.3:28129094-28129113 | None:intergenic | 35.0% | |
| ATCTTCCTGCAAAGTTTCAT+CGG | - | chr5.3:28128776-28128795 | None:intergenic | 35.0% | |
| ATCTTGAGTCTCTACAACAT+TGG | - | chr5.3:28127509-28127528 | None:intergenic | 35.0% | |
| GAAAACGTGGATAAAGTTAC+TGG | + | chr5.3:28128568-28128587 | MS.gene001150:CDS | 35.0% | |
| GAAGGTAATGATAACATTGC+GGG | + | chr5.3:28128805-28128824 | MS.gene001150:CDS | 35.0% | |
| GCAATGTTATCATTACCTTC+AGG | - | chr5.3:28128805-28128824 | None:intergenic | 35.0% | |
| GTTGTCTCAACATGAAGATA+TGG | + | chr5.3:28128834-28128853 | MS.gene001150:CDS | 35.0% | |
| TACCAAGAAGAAGATTGTAG+AGG | + | chr5.3:28129089-28129108 | MS.gene001150:CDS | 35.0% | |
| TCAACATGAAGATATGGATC+TGG | + | chr5.3:28128840-28128859 | MS.gene001150:CDS | 35.0% | |
| TCTTGAGTCTCTACAACATT+GGG | - | chr5.3:28127508-28127527 | None:intergenic | 35.0% | |
| TGAAACTTTGCAGGAAGATA+TGG | + | chr5.3:28128777-28128796 | MS.gene001150:CDS | 35.0% | |
| TGTGTTTGCTTCATGTTCAA+GGG | + | chr5.3:28128113-28128132 | MS.gene001150:intron | 35.0% | |
| ! | AACATGTCTGGAATCACTTT+AGG | + | chr5.3:28127915-28127934 | MS.gene001150:intron | 35.0% |
| ! | CGAGTTAGAATTCATTTTGG+AGG | + | chr5.3:28127863-28127882 | MS.gene001150:intron | 35.0% |
| ! | TGGTAACTTGTGTAGTAGTA+GGG | + | chr5.3:28129182-28129201 | MS.gene001150:CDS | 35.0% |
| !! | AAATGGTTTTTCTTCTGAGG+TGG | + | chr5.3:28127579-28127598 | MS.gene001150:intron | 35.0% |
| !!! | AATGGTTTTTCTTCTGAGGT+GGG | + | chr5.3:28127580-28127599 | MS.gene001150:intron | 35.0% |
| !!! | TCTCGTCAACTGTTTTTTTG+TGG | - | chr5.3:28128618-28128637 | None:intergenic | 35.0% |
| !!! | TTTTGATTTGTCTCAACCAC+AGG | + | chr5.3:28128885-28128904 | MS.gene001150:CDS | 35.0% |
| AACCTCAATTATGGTGTCTG+TGG | - | chr5.3:28128201-28128220 | None:intergenic | 40.0% | |
| AATCAAACGAGCCTAGAGAA+GGG | - | chr5.3:28127635-28127654 | None:intergenic | 40.0% | |
| AGTGGAGATCTAACACTATC+TGG | - | chr5.3:28129012-28129031 | None:intergenic | 40.0% | |
| CAGTTGGATTTCCACACAAT+TGG | + | chr5.3:28128509-28128528 | MS.gene001150:CDS | 40.0% | |
| CATGAAGATATGGATCTGGA+AGG | + | chr5.3:28128844-28128863 | MS.gene001150:CDS | 40.0% | |
| GTGGAAATCCAACTGAGAAA+CGG | - | chr5.3:28128504-28128523 | None:intergenic | 40.0% | |
| GTGTGTTTGCTTCATGTTCA+AGG | + | chr5.3:28128112-28128131 | MS.gene001150:intron | 40.0% | |
| TGGTACGTTTCTTATCCTTG+CGG | - | chr5.3:28129074-28129093 | None:intergenic | 40.0% | |
| TTAGAAATCACGTCAACCTG+TGG | - | chr5.3:28128904-28128923 | None:intergenic | 40.0% | |
| TTCCACAGACACCATAATTG+AGG | + | chr5.3:28128196-28128215 | MS.gene001150:intron | 40.0% | |
| ! | AAGCTATTACTTGGAGCATC+AGG | - | chr5.3:28128164-28128183 | None:intergenic | 40.0% |
| ! | ATCTAACACTATCTGGCTCA+TGG | - | chr5.3:28129005-28129024 | None:intergenic | 40.0% |
| ! | CAGGTTGACGTGATTTCTAA+TGG | + | chr5.3:28128904-28128923 | MS.gene001150:CDS | 40.0% |
| ! | CATGCATGAGATTTAGAGTC+TGG | + | chr5.3:28128302-28128321 | MS.gene001150:intron | 40.0% |
| ! | CTGGTAACTTGTGTAGTAGT+AGG | + | chr5.3:28129181-28129200 | MS.gene001150:CDS | 40.0% |
| ! | TCTAACACTATCTGGCTCAT+GGG | - | chr5.3:28129004-28129023 | None:intergenic | 40.0% |
| !! | AAGCATCGTGTGACTTTTCT+TGG | - | chr5.3:28129226-28129245 | None:intergenic | 40.0% |
| !!! | ACCATTTTGAGATGTACGTG+AGG | - | chr5.3:28127566-28127585 | None:intergenic | 40.0% |
| !!! | TTCATCGGCTGCTTTTTTGA+GGG | - | chr5.3:28128761-28128780 | None:intergenic | 40.0% |
| !!! | TTTCATCGGCTGCTTTTTTG+AGG | - | chr5.3:28128762-28128781 | None:intergenic | 40.0% |
| AACAGTTGACGAGACTTCAC+AGG | + | chr5.3:28128624-28128643 | MS.gene001150:CDS | 45.0% | |
| AGCCTGGTAGATCTTCTAAC+AGG | - | chr5.3:28129288-28129307 | None:intergenic | 45.0% | |
| CAATCAAACGAGCCTAGAGA+AGG | - | chr5.3:28127636-28127655 | None:intergenic | 45.0% | |
| CAGGAAGATATGGAACCTGA+AGG | + | chr5.3:28128787-28128806 | MS.gene001150:CDS | 45.0% | |
| CGAATTATCAAGTGACCGCA+AGG | + | chr5.3:28129056-28129075 | MS.gene001150:CDS | 45.0% | |
| CTCCTGTTAGAAGATCTACC+AGG | + | chr5.3:28129283-28129302 | MS.gene001150:CDS | 45.0% | |
| GCCTCACGTACATCTCAAAA+TGG | + | chr5.3:28127562-28127581 | MS.gene001150:CDS | 45.0% | |
| GGCATCACTGTAATGTTCCA+CGG | + | chr5.3:28127529-28127548 | MS.gene001150:CDS | 45.0% | |
| ! | GGTTCAAGTTCAATCCACTG+TGG | - | chr5.3:28128143-28128162 | None:intergenic | 45.0% |
| ! | GTCTGGCAATTTTGTCTGAC+AGG | + | chr5.3:28128319-28128338 | MS.gene001150:intron | 45.0% |
| ACTCCTCTTGACCCTTCTCT+AGG | + | chr5.3:28127621-28127640 | MS.gene001150:intron | 50.0% | |
| AGCAGCCGATGAAACTTTGC+AGG | + | chr5.3:28128768-28128787 | MS.gene001150:CDS | 50.0% | |
| GAGACTTCACAGGAAGCTGA+AGG | + | chr5.3:28128634-28128653 | MS.gene001150:CDS | 50.0% | |
| GTATGTCGCCGTTTCTCAGT+TGG | + | chr5.3:28128493-28128512 | MS.gene001150:CDS | 50.0% | |
| TGTTCAAGGGTCATCCACAG+TGG | + | chr5.3:28128126-28128145 | MS.gene001150:intron | 50.0% | |
| ! | ACACTATCTGGCTCATGGGT+AGG | - | chr5.3:28129000-28129019 | None:intergenic | 50.0% |
| ! | GTGAGGCGTTAATCAAACCG+TGG | - | chr5.3:28127549-28127568 | None:intergenic | 50.0% |
| AACCGGCGCAATCACACACA+AGG | - | chr5.3:28128428-28128447 | None:intergenic | 55.0% | |
| ACCCTTGTGTGTGATTGCGC+CGG | + | chr5.3:28128423-28128442 | MS.gene001150:intron | 55.0% | |
| ACCGGCGCAATCACACACAA+GGG | - | chr5.3:28128427-28128446 | None:intergenic | 55.0% | |
| GAGCCTAGAGAAGGGTCAAG+AGG | - | chr5.3:28127627-28127646 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 28127469 | 28129326 | 28127469 | ID=MS.gene001150 |
| chr5.3 | mRNA | 28127469 | 28129326 | 28127469 | ID=MS.gene001150.t1;Parent=MS.gene001150 |
| chr5.3 | exon | 28127469 | 28127597 | 28127469 | ID=MS.gene001150.t1.exon1;Parent=MS.gene001150.t1 |
| chr5.3 | CDS | 28127469 | 28127597 | 28127469 | ID=cds.MS.gene001150.t1;Parent=MS.gene001150.t1 |
| chr5.3 | exon | 28128493 | 28129326 | 28128493 | ID=MS.gene001150.t1.exon2;Parent=MS.gene001150.t1 |
| chr5.3 | CDS | 28128493 | 28129326 | 28128493 | ID=cds.MS.gene001150.t1;Parent=MS.gene001150.t1 |
| Gene Sequence |
| Protein sequence |
