AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene001180

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001180.t1 MTR_2g061450 91.204 432 38 0 1 432 1 432 0.0 803
MS.gene001180.t1 MTR_2g060620 86.486 111 13 1 302 412 61 169 3.05e-60 194
MS.gene001180.t1 MTR_5g094610 23.600 250 180 6 122 361 214 462 1.03e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001180.t1 AT1G74120 51.816 413 198 1 20 432 34 445 2.15e-153 442
MS.gene001180.t1 AT5G54180 28.000 225 150 6 137 354 253 472 2.96e-14 75.1
MS.gene001180.t1 AT1G62010 23.925 372 211 13 29 383 72 388 1.84e-12 68.9
MS.gene001180.t1 AT3G46950 25.294 340 230 10 29 359 86 410 1.97e-12 69.3
MS.gene001180.t1 AT5G23930 22.047 381 252 12 29 388 89 445 3.83e-11 65.1
MS.gene001180.t1 AT5G55580 24.268 239 172 7 122 351 207 445 6.07e-11 64.7

Find 106 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAGCTTGGATTTGAATTT+TGG 0.098657 2.1:-33931834 None:intergenic
AACCCCAATTTCTACATAAT+TGG 0.109235 2.1:+33931799 MS.gene001180:CDS
ATTCCCAATTATGTAGAAAT+TGG 0.124209 2.1:-33931803 None:intergenic
TGTTAGGGATCCAAGGATTT+TGG 0.152438 2.1:+33932205 MS.gene001180:CDS
AAATTTCAAATGGGTTTAAA+TGG 0.193455 2.1:-33931908 None:intergenic
TGGATTGTTTCCTAGGGTTT+TGG 0.194253 2.1:+33932097 MS.gene001180:CDS
ATTCATGAGCTGAGGGGTTT+GGG 0.198657 2.1:+33932152 MS.gene001180:CDS
CATGTTCATCACATCTTTAC+TGG 0.212061 2.1:-33932777 None:intergenic
AATCTTGAACAAGTGAAATT+AGG 0.234038 2.1:-33931761 None:intergenic
TCAAGATTGCCCTTCTGTTT+TGG 0.238186 2.1:+33931776 MS.gene001180:CDS
TTCTGAAAATGTTGTGATTA+GGG 0.263086 2.1:+33931965 MS.gene001180:CDS
GATTCATGAGCTGAGGGGTT+TGG 0.264040 2.1:+33932151 MS.gene001180:CDS
ATTTCTACATAATTGGGAAT+TGG 0.292126 2.1:+33931806 MS.gene001180:CDS
TTTCTGAAAATGTTGTGATT+AGG 0.296124 2.1:+33931964 MS.gene001180:CDS
AAATGTTGTGATTAGGGTTT+TGG 0.299994 2.1:+33931971 MS.gene001180:CDS
CAACACTATATTTCATCCTT+TGG 0.303808 2.1:-33932472 None:intergenic
TTCCCAATTATGTAGAAATT+GGG 0.304182 2.1:-33931802 None:intergenic
GAGGTTGATTGGTTATGATT+TGG 0.308965 2.1:+33932418 MS.gene001180:CDS
AGGTCAGGGCGGAGGTTGTT+AGG 0.309694 2.1:+33932189 MS.gene001180:CDS
TTGAAAGGAAAAGGTGCAAT+TGG 0.315697 2.1:+33932575 MS.gene001180:CDS
AAATTCACACCCAAGTATTC+AGG 0.317076 2.1:-33932512 None:intergenic
GATGAAATTGATAGAGTTGT+TGG 0.328831 2.1:+33932077 MS.gene001180:CDS
TTTCTACATAATTGGGAATT+GGG 0.332347 2.1:+33931807 MS.gene001180:CDS
GAGTTGTTGGATTGTTTCCT+AGG 0.358743 2.1:+33932090 MS.gene001180:CDS
GATGAACATGAAGGCTTTCA+TGG 0.369314 2.1:+33932790 MS.gene001180:CDS
GAAGGAAAAGAGGGTTGTTA+AGG 0.369880 2.1:-33931721 None:intergenic
ACCCCAATTTCTACATAATT+GGG 0.391543 2.1:+33931800 MS.gene001180:CDS
TCTCCCAGTGCAGTTGTAAT+GGG 0.393930 2.1:+33931999 MS.gene001180:CDS
GGTGCAATTGGGTTTGAAGT+TGG 0.399622 2.1:+33932587 MS.gene001180:CDS
ACACGTTGGCGATCATCAAC+GGG 0.406673 2.1:-33931866 None:intergenic
ACTCTATCAATTTCATCCTT+AGG 0.408293 2.1:-33932071 None:intergenic
TGGGATTTGGTATTGAGGAT+AGG 0.409253 2.1:+33932117 MS.gene001180:CDS
GAAAGCATCCCGTTGGACTT+TGG 0.413704 2.1:+33932723 MS.gene001180:CDS
TCACATCTTTACTGGTTTGA+GGG 0.416518 2.1:-33932769 None:intergenic
AATTCGTAGGGATGCTTTGA+AGG 0.418729 2.1:+33932379 MS.gene001180:CDS
TGGAAGGAACCGAGGTTGAT+TGG 0.420423 2.1:+33932407 MS.gene001180:CDS
ATCACATCTTTACTGGTTTG+AGG 0.423102 2.1:-33932770 None:intergenic
ATCTCCCAGTGCAGTTGTAA+TGG 0.423551 2.1:+33931998 MS.gene001180:CDS
TGAAAGGAAAAGGTGCAATT+GGG 0.424464 2.1:+33932576 MS.gene001180:CDS
TGTTGCAGAGTTTGAAATGC+AGG 0.431814 2.1:+33932264 MS.gene001180:CDS
CGGGGAAGCATTAGAAAGCT+TGG 0.440265 2.1:-33931847 None:intergenic
GCATGAAGTTCCTGAATACT+TGG 0.443861 2.1:+33932502 MS.gene001180:CDS
CCCCTCAGCTCATGAATCAA+TGG 0.448660 2.1:-33932146 None:intergenic
CAGCCGAGAGGTCAGGGCGG+AGG 0.450679 2.1:+33932181 MS.gene001180:CDS
ATCTCCATTCCCAAAATCCT+TGG 0.454905 2.1:-33932215 None:intergenic
TACTCAATCACATTGTATCT+AGG 0.456654 2.1:-33932551 None:intergenic
GCTAATCTATTCCAATCCTT+CGG 0.461040 2.1:+33931618 MS.gene001180:CDS
TTGTTAAGGATTGGCGAAGG+TGG 0.462969 2.1:-33931707 None:intergenic
GCGGTCACGGTTTAATTCGT+AGG 0.464329 2.1:+33932366 MS.gene001180:CDS
TGTTAAGGATTGGCGAAGGT+GGG 0.467753 2.1:-33931706 None:intergenic
GTTGATTGGTTATGATTTGG+AGG 0.468999 2.1:+33932421 MS.gene001180:CDS
TTATAAAATCTAAGCCTAGT+TGG 0.470603 2.1:-33932629 None:intergenic
AAACGAGGAAGAGAAGAGGA+AGG 0.471169 2.1:-33931645 None:intergenic
CTCCGCCCTGACCTCTCGGC+TGG 0.473380 2.1:-33932180 None:intergenic
ATTGAGTATTTGAAAGGAAA+AGG 0.475963 2.1:+33932566 MS.gene001180:CDS
GGTCAGGGCGGAGGTTGTTA+GGG 0.481020 2.1:+33932190 MS.gene001180:CDS
ATGAGATGTTTGGTGAGAAA+TGG 0.482682 2.1:-33931574 None:intergenic
AACATCTTCCAAAGTCCAAC+GGG 0.486105 2.1:-33932731 None:intergenic
AAAAGAGGGTTGTTAAGGAT+TGG 0.487510 2.1:-33931716 None:intergenic
GTGGATTGTTTGTGCGGTCA+CGG 0.490001 2.1:+33932353 MS.gene001180:CDS
AGGAAGGAAAACCGAAGGAT+TGG 0.491372 2.1:-33931629 None:intergenic
TGTGGAATTTGGGATTCCTA+AGG 0.495755 2.1:+33932055 MS.gene001180:CDS
AGTTGTTGGATTGTTTCCTA+GGG 0.504801 2.1:+33932091 MS.gene001180:CDS
AATGTGATTGAGTATTTGAA+AGG 0.505696 2.1:+33932560 MS.gene001180:CDS
AACACGTTGGCGATCATCAA+CGG 0.506194 2.1:-33931867 None:intergenic
TTTGAGGGAACTTCGGTGGT+TGG 0.508881 2.1:-33932754 None:intergenic
TTGGCGAAGGTGGGATGGAT+CGG 0.509984 2.1:-33931697 None:intergenic
TCATGGACTCGTTGGTGTAG+TGG 0.510176 2.1:+33932807 MS.gene001180:CDS
CGGTCACGGTTTAATTCGTA+GGG 0.517633 2.1:+33932367 MS.gene001180:CDS
TTCATGAGCTGAGGGGTTTG+GGG 0.523718 2.1:+33932153 MS.gene001180:CDS
GCCATTGATTCATGAGCTGA+GGG 0.528936 2.1:+33932145 MS.gene001180:CDS
TTACTGGTTTGAGGGAACTT+CGG 0.536810 2.1:-33932761 None:intergenic
CGGAGGTTGTTAGGGATCCA+AGG 0.538817 2.1:+33932198 MS.gene001180:CDS
TCCCAATTATGTAGAAATTG+GGG 0.539066 2.1:-33931801 None:intergenic
AGATGTTTGGTGAGAAATGG+TGG 0.539546 2.1:-33931571 None:intergenic
GAGGAAACGAGGAAGAGAAG+AGG 0.544818 2.1:-33931649 None:intergenic
AAGGATTGGCGAAGGTGGGA+TGG 0.547823 2.1:-33931702 None:intergenic
CAACCTCCGCCCTGACCTCT+CGG 0.563699 2.1:-33932184 None:intergenic
ATACCAAATCCCAAAACCCT+AGG 0.565652 2.1:-33932107 None:intergenic
GAAGGCTTTCATGGACTCGT+TGG 0.570328 2.1:+33932799 MS.gene001180:CDS
GAAGGTGTTAGTTGAAGCAA+TGG 0.571180 2.1:-33931547 None:intergenic
CTGGTTTGAGGGAACTTCGG+TGG 0.572836 2.1:-33932758 None:intergenic
AATTGGGGTTCCAAAACAGA+AGG 0.574774 2.1:-33931786 None:intergenic
TGCTTTGAAGGTGTTGTGGA+AGG 0.580553 2.1:+33932391 MS.gene001180:CDS
GGGATGGATCGGAATTGAAG+AGG 0.584178 2.1:-33931686 None:intergenic
CATGAAGTTCCTGAATACTT+GGG 0.593187 2.1:+33932503 MS.gene001180:CDS
AGCCATTGATTCATGAGCTG+AGG 0.597553 2.1:+33932144 MS.gene001180:CDS
CTCCCAGTGCAGTTGTAATG+GGG 0.598682 2.1:+33932000 MS.gene001180:CDS
AAGACATTATCAAACCAACT+AGG 0.602970 2.1:+33932615 MS.gene001180:CDS
GTTAAAAGAAAGCATCCCGT+TGG 0.604141 2.1:+33932716 MS.gene001180:CDS
TTGGTGAGAAATGGTGGAGA+AGG 0.607502 2.1:-33931565 None:intergenic
TAAAGATGTGATGAACATGA+AGG 0.609470 2.1:+33932781 MS.gene001180:CDS
GGGTTGTTAAGGATTGGCGA+AGG 0.612411 2.1:-33931710 None:intergenic
GGGATGCTTTGAAGGTGTTG+TGG 0.613256 2.1:+33932387 MS.gene001180:CDS
AGAAGAGGAAGGAAAACCGA+AGG 0.618756 2.1:-33931634 None:intergenic
GTTGCAGAGTTTGAAATGCA+GGG 0.619561 2.1:+33932265 MS.gene001180:CDS
GAACATCTTCCAAAGTCCAA+CGG 0.624427 2.1:-33932732 None:intergenic
CTGAGAGTGGATTGTTTGTG+CGG 0.636549 2.1:+33932347 MS.gene001180:CDS
TCCCAGTGCAGTTGTAATGG+GGG 0.640308 2.1:+33932001 MS.gene001180:CDS
AAATCATAACCAATCAACCT+CGG 0.663302 2.1:-33932416 None:intergenic
TGTAGAACAACGCAACACGT+TGG 0.665740 2.1:-33931880 None:intergenic
ATTGGGGTTCCAAAACAGAA+GGG 0.683492 2.1:-33931785 None:intergenic
CCATTGATTCATGAGCTGAG+GGG 0.690477 2.1:+33932146 MS.gene001180:CDS
TTCCAGCCGAGAGGTCAGGG+CGG 0.702811 2.1:+33932178 MS.gene001180:CDS
CACGTTGGCGATCATCAACG+GGG 0.742441 2.1:-33931865 None:intergenic
AGGTGTTGTGGAAGGAACCG+AGG 0.744040 2.1:+33932399 MS.gene001180:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTCAAATGGGTTTAAA+TGG - chr2.1:33931911-33931930 None:intergenic 20.0%
!!! AATTAAAGAGAAGATTTTTG+GGG + chr2.1:33932292-33932311 MS.gene001180:CDS 20.0%
!!! ATTTTATGGGAAATTTCAAA+TGG - chr2.1:33931921-33931940 None:intergenic 20.0%
!!! CAATTAAAGAGAAGATTTTT+GGG + chr2.1:33932291-33932310 MS.gene001180:CDS 20.0%
!!! CATATATTTTTTCACATTCA+GGG - chr2.1:33932673-33932692 None:intergenic 20.0%
!!! CCATAAAATTGAGATTTTTA+GGG + chr2.1:33931932-33931951 MS.gene001180:CDS 20.0%
!!! CCTAAAAATCTCAATTTTAT+GGG - chr2.1:33931934-33931953 None:intergenic 20.0%
!!! TTTTATGGGAAATTTCAAAT+GGG - chr2.1:33931920-33931939 None:intergenic 20.0%
! AATCTTGAACAAGTGAAATT+AGG - chr2.1:33931764-33931783 None:intergenic 25.0%
! AATGTGATTGAGTATTTGAA+AGG + chr2.1:33932560-33932579 MS.gene001180:CDS 25.0%
! ATTCCCAATTATGTAGAAAT+TGG - chr2.1:33931806-33931825 None:intergenic 25.0%
! ATTGAGTATTTGAAAGGAAA+AGG + chr2.1:33932566-33932585 MS.gene001180:CDS 25.0%
! ATTTCTACATAATTGGGAAT+TGG + chr2.1:33931806-33931825 MS.gene001180:CDS 25.0%
! CCTGAATGTGAAAAAATATA+TGG + chr2.1:33932671-33932690 MS.gene001180:CDS 25.0%
! TTATAAAATCTAAGCCTAGT+TGG - chr2.1:33932632-33932651 None:intergenic 25.0%
! TTCCCAATTATGTAGAAATT+GGG - chr2.1:33931805-33931824 None:intergenic 25.0%
! TTTCTACATAATTGGGAATT+GGG + chr2.1:33931807-33931826 MS.gene001180:CDS 25.0%
!! AGATTGAGTTTTTAATCCAA+AGG + chr2.1:33932456-33932475 MS.gene001180:CDS 25.0%
!! CCCATAAAATTGAGATTTTT+AGG + chr2.1:33931931-33931950 MS.gene001180:CDS 25.0%
!! CCCTAAAAATCTCAATTTTA+TGG - chr2.1:33931935-33931954 None:intergenic 25.0%
!! GCAATTAAAGAGAAGATTTT+TGG + chr2.1:33932290-33932309 MS.gene001180:CDS 25.0%
!! TTCTGAAAATGTTGTGATTA+GGG + chr2.1:33931965-33931984 MS.gene001180:CDS 25.0%
!! TTGATTTTCTTGTGGAATTT+GGG + chr2.1:33932045-33932064 MS.gene001180:CDS 25.0%
!! TTTCTGAAAATGTTGTGATT+AGG + chr2.1:33931964-33931983 MS.gene001180:CDS 25.0%
!!! ATTGAGATTTTTAGGGATTT+AGG + chr2.1:33931939-33931958 MS.gene001180:CDS 25.0%
!!! ATTGATTTTCTTGTGGAATT+TGG + chr2.1:33932044-33932063 MS.gene001180:CDS 25.0%
!!! CCATATATTTTTTCACATTC+AGG - chr2.1:33932674-33932693 None:intergenic 25.0%
!!! TTGAGATTTTTAGGGATTTA+GGG + chr2.1:33931940-33931959 MS.gene001180:CDS 25.0%
AAATCATAACCAATCAACCT+CGG - chr2.1:33932419-33932438 None:intergenic 30.0%
AACCCCAATTTCTACATAAT+TGG + chr2.1:33931799-33931818 MS.gene001180:CDS 30.0%
AAGACATTATCAAACCAACT+AGG + chr2.1:33932615-33932634 MS.gene001180:CDS 30.0%
ACCCCAATTTCTACATAATT+GGG + chr2.1:33931800-33931819 MS.gene001180:CDS 30.0%
ACTCTATCAATTTCATCCTT+AGG - chr2.1:33932074-33932093 None:intergenic 30.0%
CAACACTATATTTCATCCTT+TGG - chr2.1:33932475-33932494 None:intergenic 30.0%
GATGAAATTGATAGAGTTGT+TGG + chr2.1:33932077-33932096 MS.gene001180:CDS 30.0%
TAAAGATGTGATGAACATGA+AGG + chr2.1:33932781-33932800 MS.gene001180:CDS 30.0%
TACTCAATCACATTGTATCT+AGG - chr2.1:33932554-33932573 None:intergenic 30.0%
TCCCAATTATGTAGAAATTG+GGG - chr2.1:33931804-33931823 None:intergenic 30.0%
! AAATGTTGTGATTAGGGTTT+TGG + chr2.1:33931971-33931990 MS.gene001180:CDS 30.0%
! AGTGAAATTAGGTTTTTCTG+TGG - chr2.1:33931753-33931772 None:intergenic 30.0%
! ATATGGCAGATTTTCAACAA+AGG + chr2.1:33932688-33932707 MS.gene001180:CDS 30.0%
! ATTGAAGAGGAAGTGATTTT+TGG - chr2.1:33931676-33931695 None:intergenic 30.0%
! GAAAGCTTGGATTTGAATTT+TGG - chr2.1:33931837-33931856 None:intergenic 30.0%
! GTTTTCTGTAATGAGATGTT+TGG - chr2.1:33931587-33931606 None:intergenic 30.0%
! GTTTTTCTGTGGAATTTTGA+AGG - chr2.1:33931742-33931761 None:intergenic 30.0%
!! TGGAATTTTGAAGGAAAAGA+GGG - chr2.1:33931733-33931752 None:intergenic 30.0%
!!! ATTTTTTCACATTCAGGGTA+AGG - chr2.1:33932668-33932687 None:intergenic 30.0%
!!! TAGTGTCATTGATTTTCTTG+TGG + chr2.1:33932037-33932056 MS.gene001180:CDS 30.0%
AAAAGAGGGTTGTTAAGGAT+TGG - chr2.1:33931719-33931738 None:intergenic 35.0%
AAATTCACACCCAAGTATTC+AGG - chr2.1:33932515-33932534 None:intergenic 35.0%
AGTTGTTGGATTGTTTCCTA+GGG + chr2.1:33932091-33932110 MS.gene001180:CDS 35.0%
CATGAAGTTCCTGAATACTT+GGG + chr2.1:33932503-33932522 MS.gene001180:CDS 35.0%
CATGTTCATCACATCTTTAC+TGG - chr2.1:33932780-33932799 None:intergenic 35.0%
GCTAATCTATTCCAATCCTT+CGG + chr2.1:33931618-33931637 MS.gene001180:CDS 35.0%
! ATGAGATGTTTGGTGAGAAA+TGG - chr2.1:33931577-33931596 None:intergenic 35.0%
! GAGGTTGATTGGTTATGATT+TGG + chr2.1:33932418-33932437 MS.gene001180:CDS 35.0%
! GTTGATTGGTTATGATTTGG+AGG + chr2.1:33932421-33932440 MS.gene001180:CDS 35.0%
! TGAAAGGAAAAGGTGCAATT+GGG + chr2.1:33932576-33932595 MS.gene001180:CDS 35.0%
! TTGAAAGGAAAAGGTGCAAT+TGG + chr2.1:33932575-33932594 MS.gene001180:CDS 35.0%
!! AAAGAGAAGATTTTTGGGGA+GGG + chr2.1:33932296-33932315 MS.gene001180:CDS 35.0%
!! ATCACATCTTTACTGGTTTG+AGG - chr2.1:33932773-33932792 None:intergenic 35.0%
!! GTGGAATTTTGAAGGAAAAG+AGG - chr2.1:33931734-33931753 None:intergenic 35.0%
!! TAAAGAGAAGATTTTTGGGG+AGG + chr2.1:33932295-33932314 MS.gene001180:CDS 35.0%
!! TCACATCTTTACTGGTTTGA+GGG - chr2.1:33932772-33932791 None:intergenic 35.0%
AACATCTTCCAAAGTCCAAC+GGG - chr2.1:33932734-33932753 None:intergenic 40.0%
AATTCGTAGGGATGCTTTGA+AGG + chr2.1:33932379-33932398 MS.gene001180:CDS 40.0%
AATTGGGGTTCCAAAACAGA+AGG - chr2.1:33931789-33931808 None:intergenic 40.0%
ATACCAAATCCCAAAACCCT+AGG - chr2.1:33932110-33932129 None:intergenic 40.0%
ATCTCCATTCCCAAAATCCT+TGG - chr2.1:33932218-33932237 None:intergenic 40.0%
ATTGGGGTTCCAAAACAGAA+GGG - chr2.1:33931788-33931807 None:intergenic 40.0%
GAACATCTTCCAAAGTCCAA+CGG - chr2.1:33932735-33932754 None:intergenic 40.0%
GAAGGAAAAGAGGGTTGTTA+AGG - chr2.1:33931724-33931743 None:intergenic 40.0%
GAGTTGTTGGATTGTTTCCT+AGG + chr2.1:33932090-33932109 MS.gene001180:CDS 40.0%
GATGAACATGAAGGCTTTCA+TGG + chr2.1:33932790-33932809 MS.gene001180:CDS 40.0%
GCATGAAGTTCCTGAATACT+TGG + chr2.1:33932502-33932521 MS.gene001180:CDS 40.0%
! AGATGTTTGGTGAGAAATGG+TGG - chr2.1:33931574-33931593 None:intergenic 40.0%
! GGATTGTTTCCTAGGGTTTT+GGG + chr2.1:33932098-33932117 MS.gene001180:CDS 40.0%
! GTTAGGGATCCAAGGATTTT+GGG + chr2.1:33932206-33932225 MS.gene001180:CDS 40.0%
! GTTGCAGAGTTTGAAATGCA+GGG + chr2.1:33932265-33932284 MS.gene001180:CDS 40.0%
! TCAAGATTGCCCTTCTGTTT+TGG + chr2.1:33931776-33931795 MS.gene001180:CDS 40.0%
! TGGAGATTGGGGAATTTTCA+CGG + chr2.1:33932231-33932250 MS.gene001180:CDS 40.0%
! TGGATTGTTTCCTAGGGTTT+TGG + chr2.1:33932097-33932116 MS.gene001180:CDS 40.0%
! TGGGATTTGGTATTGAGGAT+AGG + chr2.1:33932117-33932136 MS.gene001180:CDS 40.0%
! TGTGGAATTTGGGATTCCTA+AGG + chr2.1:33932055-33932074 MS.gene001180:CDS 40.0%
! TGTTAGGGATCCAAGGATTT+TGG + chr2.1:33932205-33932224 MS.gene001180:CDS 40.0%
! TGTTGCAGAGTTTGAAATGC+AGG + chr2.1:33932264-33932283 MS.gene001180:CDS 40.0%
!! AGAGGAAGTGATTTTTGGAG+AGG - chr2.1:33931671-33931690 None:intergenic 40.0%
!! AGGATTTTGGGAATGGAGAT+TGG + chr2.1:33932218-33932237 MS.gene001180:CDS 40.0%
!! GAAGGTGTTAGTTGAAGCAA+TGG - chr2.1:33931550-33931569 None:intergenic 40.0%
!! GATTTTGGGAATGGAGATTG+GGG + chr2.1:33932220-33932239 MS.gene001180:CDS 40.0%
!! GGATTTTGGGAATGGAGATT+GGG + chr2.1:33932219-33932238 MS.gene001180:CDS 40.0%
!! GTTAAAAGAAAGCATCCCGT+TGG + chr2.1:33932716-33932735 MS.gene001180:CDS 40.0%
!! TGATTTTTGGAGAGGAAACG+AGG - chr2.1:33931663-33931682 None:intergenic 40.0%
!! TGTTGTGATTAGGGTTTTGG+AGG + chr2.1:33931974-33931993 MS.gene001180:CDS 40.0%
!! TTACTGGTTTGAGGGAACTT+CGG - chr2.1:33932764-33932783 None:intergenic 40.0%
!!! GGTTTTGGGATTTGGTATTG+AGG + chr2.1:33932112-33932131 MS.gene001180:CDS 40.0%
!!! TTTCCTAGGGTTTTGGGATT+TGG + chr2.1:33932104-33932123 MS.gene001180:CDS 40.0%
!!! TTTTGAAGTGAAGCTGAGAG+TGG + chr2.1:33932334-33932353 MS.gene001180:CDS 40.0%
AAACGAGGAAGAGAAGAGGA+AGG - chr2.1:33931648-33931667 None:intergenic 45.0%
AACACGTTGGCGATCATCAA+CGG - chr2.1:33931870-33931889 None:intergenic 45.0%
AGAAGAGGAAGGAAAACCGA+AGG - chr2.1:33931637-33931656 None:intergenic 45.0%
AGGAAGGAAAACCGAAGGAT+TGG - chr2.1:33931632-33931651 None:intergenic 45.0%
ATCTCCCAGTGCAGTTGTAA+TGG + chr2.1:33931998-33932017 MS.gene001180:CDS 45.0%
ATTCATGAGCTGAGGGGTTT+GGG + chr2.1:33932152-33932171 MS.gene001180:CDS 45.0%
CGGTCACGGTTTAATTCGTA+GGG + chr2.1:33932367-33932386 MS.gene001180:CDS 45.0%
TCTCCCAGTGCAGTTGTAAT+GGG + chr2.1:33931999-33932018 MS.gene001180:CDS 45.0%
TGTAGAACAACGCAACACGT+TGG - chr2.1:33931883-33931902 None:intergenic 45.0%
! GGTGCAATTGGGTTTGAAGT+TGG + chr2.1:33932587-33932606 MS.gene001180:CDS 45.0%
! TGTTAAGGATTGGCGAAGGT+GGG - chr2.1:33931709-33931728 None:intergenic 45.0%
! TTGGTGAGAAATGGTGGAGA+AGG - chr2.1:33931568-33931587 None:intergenic 45.0%
! TTGTTAAGGATTGGCGAAGG+TGG - chr2.1:33931710-33931729 None:intergenic 45.0%
!! AGCCATTGATTCATGAGCTG+AGG + chr2.1:33932144-33932163 MS.gene001180:CDS 45.0%
!! CCATTGATTCATGAGCTGAG+GGG + chr2.1:33932146-33932165 MS.gene001180:CDS 45.0%
!! CTGAGAGTGGATTGTTTGTG+CGG + chr2.1:33932347-33932366 MS.gene001180:CDS 45.0%
!! GAAGATTTTTGGGGAGGGTT+TGG + chr2.1:33932301-33932320 MS.gene001180:CDS 45.0%
!! GCCATTGATTCATGAGCTGA+GGG + chr2.1:33932145-33932164 MS.gene001180:CDS 45.0%
!! GGATCCAAGGATTTTGGGAA+TGG + chr2.1:33932211-33932230 MS.gene001180:CDS 45.0%
!! TGCTTTGAAGGTGTTGTGGA+AGG + chr2.1:33932391-33932410 MS.gene001180:CDS 45.0%
!! TTTTGGGGAGGGTTTGGTTA+GGG + chr2.1:33932307-33932326 MS.gene001180:CDS 45.0%
!! TTTTTGGGGAGGGTTTGGTT+AGG + chr2.1:33932306-33932325 MS.gene001180:CDS 45.0%
ACACGTTGGCGATCATCAAC+GGG - chr2.1:33931869-33931888 None:intergenic 50.0%
CCCCTCAGCTCATGAATCAA+TGG - chr2.1:33932149-33932168 None:intergenic 50.0%
CTCCCAGTGCAGTTGTAATG+GGG + chr2.1:33932000-33932019 MS.gene001180:CDS 50.0%
GAAGGCTTTCATGGACTCGT+TGG + chr2.1:33932799-33932818 MS.gene001180:CDS 50.0%
GAGGAAACGAGGAAGAGAAG+AGG - chr2.1:33931652-33931671 None:intergenic 50.0%
GATTCATGAGCTGAGGGGTT+TGG + chr2.1:33932151-33932170 MS.gene001180:CDS 50.0%
GCGGTCACGGTTTAATTCGT+AGG + chr2.1:33932366-33932385 MS.gene001180:CDS 50.0%
GGGATGGATCGGAATTGAAG+AGG - chr2.1:33931689-33931708 None:intergenic 50.0%
TCCCAGTGCAGTTGTAATGG+GGG + chr2.1:33932001-33932020 MS.gene001180:CDS 50.0%
TCCCCCATTACAACTGCACT+GGG - chr2.1:33932005-33932024 None:intergenic 50.0%
TTCATGAGCTGAGGGGTTTG+GGG + chr2.1:33932153-33932172 MS.gene001180:CDS 50.0%
TTCCCCCATTACAACTGCAC+TGG - chr2.1:33932006-33932025 None:intergenic 50.0%
TTTGAGGGAACTTCGGTGGT+TGG - chr2.1:33932757-33932776 None:intergenic 50.0%
! GGGTTGTTAAGGATTGGCGA+AGG - chr2.1:33931713-33931732 None:intergenic 50.0%
! GTGGATTGTTTGTGCGGTCA+CGG + chr2.1:33932353-33932372 MS.gene001180:CDS 50.0%
! TGGAAGGAACCGAGGTTGAT+TGG + chr2.1:33932407-33932426 MS.gene001180:CDS 50.0%
!! CGGGGAAGCATTAGAAAGCT+TGG - chr2.1:33931850-33931869 None:intergenic 50.0%
!! GAAAGCATCCCGTTGGACTT+TGG + chr2.1:33932723-33932742 MS.gene001180:CDS 50.0%
!! GGGATGCTTTGAAGGTGTTG+TGG + chr2.1:33932387-33932406 MS.gene001180:CDS 50.0%
CACGTTGGCGATCATCAACG+GGG - chr2.1:33931868-33931887 None:intergenic 55.0%
CGGAGGTTGTTAGGGATCCA+AGG + chr2.1:33932198-33932217 MS.gene001180:CDS 55.0%
TTGGCGAAGGTGGGATGGAT+CGG - chr2.1:33931700-33931719 None:intergenic 55.0%
! AAGGATTGGCGAAGGTGGGA+TGG - chr2.1:33931705-33931724 None:intergenic 55.0%
! AGGTGTTGTGGAAGGAACCG+AGG + chr2.1:33932399-33932418 MS.gene001180:CDS 55.0%
! CTGGTTTGAGGGAACTTCGG+TGG - chr2.1:33932761-33932780 None:intergenic 55.0%
! GTTTTCCAGCCGAGAGGTCA+GGG + chr2.1:33932175-33932194 MS.gene001180:CDS 55.0%
!! TTTGGGGTTTTCCAGCCGAG+AGG + chr2.1:33932169-33932188 MS.gene001180:CDS 55.0%
AGGTCAGGGCGGAGGTTGTT+AGG + chr2.1:33932189-33932208 MS.gene001180:CDS 60.0%
GGTCAGGGCGGAGGTTGTTA+GGG + chr2.1:33932190-33932209 MS.gene001180:CDS 60.0%
! GGTTTTCCAGCCGAGAGGTC+AGG + chr2.1:33932174-33932193 MS.gene001180:CDS 60.0%
CAACCTCCGCCCTGACCTCT+CGG - chr2.1:33932187-33932206 None:intergenic 65.0%
TTCCAGCCGAGAGGTCAGGG+CGG + chr2.1:33932178-33932197 MS.gene001180:CDS 65.0%
CAGCCGAGAGGTCAGGGCGG+AGG + chr2.1:33932181-33932200 MS.gene001180:CDS 75.0%
CTCCGCCCTGACCTCTCGGC+TGG - chr2.1:33932183-33932202 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 33931528 33932826 33931528 ID=MS.gene001180
chr2.1 mRNA 33931528 33932826 33931528 ID=MS.gene001180.t1;Parent=MS.gene001180
chr2.1 exon 33931528 33932826 33931528 ID=MS.gene001180.t1.exon1;Parent=MS.gene001180.t1
chr2.1 CDS 33931528 33932826 33931528 ID=cds.MS.gene001180.t1;Parent=MS.gene001180.t1
Gene Sequence

>MS.gene001180

ATGCTTATACTCAGAAGAACCATTGCTTCAACTAACACCTTCTCCACCATTTCTCACCAAACATCTCATTACAGAAAACAAATCACACTCGCTAATCTATTCCAATCCTTCGGTTTTCCTTCCTCTTCTCTTCCTCGTTTCCTCTCCAAAAATCACTTCCTCTTCAATTCCGATCCATCCCACCTTCGCCAATCCTTAACAACCCTCTTTTCCTTCAAAATTCCACAGAAAAACCTAATTTCACTTGTTCAAGATTGCCCTTCTGTTTTGGAACCCCAATTTCTACATAATTGGGAATTGGGTTTTCCAAAATTCAAATCCAAGCTTTCTAATGCTTCCCCGTTGATGATCGCCAACGTGTTGCGTTGTTCTACAACTTTCCATTTAAACCCATTTGAAATTTCCCATAAAATTGAGATTTTTAGGGATTTAGGGTTTTCTGAAAATGTTGTGATTAGGGTTTTGGAGGAATCTCCCAGTGCAGTTGTAATGGGGGAAACTCAAATTGTTAGTGTCATTGATTTTCTTGTGGAATTTGGGATTCCTAAGGATGAAATTGATAGAGTTGTTGGATTGTTTCCTAGGGTTTTGGGATTTGGTATTGAGGATAGGCTGAAGCCATTGATTCATGAGCTGAGGGGTTTGGGGTTTTCCAGCCGAGAGGTCAGGGCGGAGGTTGTTAGGGATCCAAGGATTTTGGGAATGGAGATTGGGGAATTTTCACGGTGTTTGAAGTTGTTGCAGAGTTTGAAATGCAGGGATGCAATTAAAGAGAAGATTTTTGGGGAGGGTTTGGTTAGGGCTTGTTTTGAAGTGAAGCTGAGAGTGGATTGTTTGTGCGGTCACGGTTTAATTCGTAGGGATGCTTTGAAGGTGTTGTGGAAGGAACCGAGGTTGATTGGTTATGATTTGGAGGATATTGAGAAGAAGATTGAGTTTTTAATCCAAAGGATGAAATATAGTGTTGATTGTTTGCATGAAGTTCCTGAATACTTGGGTGTGAATTTTGAAAAACAGATTGTTCCTAGATACAATGTGATTGAGTATTTGAAAGGAAAAGGTGCAATTGGGTTTGAAGTTGGATTGAAAGACATTATCAAACCAACTAGGCTTAGATTTTATAATCTTTATGTCAAGCCTTACCCTGAATGTGAAAAAATATATGGCAGATTTTCAACAAAGGATGAAGTTAAAAGAAAGCATCCCGTTGGACTTTGGAAGATGTTCCAACCACCGAAGTTCCCTCAAACCAGTAAAGATGTGATGAACATGAAGGCTTTCATGGACTCGTTGGTGTAG
Protein sequence

>MS.gene001180.t1

MLILRRTIASTNTFSTISHQTSHYRKQITLANLFQSFGFPSSSLPRFLSKNHFLFNSDPSHLRQSLTTLFSFKIPQKNLISLVQDCPSVLEPQFLHNWELGFPKFKSKLSNASPLMIANVLRCSTTFHLNPFEISHKIEIFRDLGFSENVVIRVLEESPSAVVMGETQIVSVIDFLVEFGIPKDEIDRVVGLFPRVLGFGIEDRLKPLIHELRGLGFSSREVRAEVVRDPRILGMEIGEFSRCLKLLQSLKCRDAIKEKIFGEGLVRACFEVKLRVDCLCGHGLIRRDALKVLWKEPRLIGYDLEDIEKKIEFLIQRMKYSVDCLHEVPEYLGVNFEKQIVPRYNVIEYLKGKGAIGFEVGLKDIIKPTRLRFYNLYVKPYPECEKIYGRFSTKDEVKRKHPVGLWKMFQPPKFPQTSKDVMNMKAFMDSLV