Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001219.t1 | XP_003631014.2 | 92.7 | 109 | 8 | 0 | 3 | 111 | 875 | 983 | 2.40E-50 | 208.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001219.t1 | Q9C5U0 | 69.7 | 109 | 26 | 1 | 3 | 111 | 959 | 1060 | 3.1e-35 | 149.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001219.t1 | G7LCC4 | 92.7 | 109 | 8 | 0 | 3 | 111 | 632 | 740 | 1.7e-50 | 208.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene001219.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001219.t1 | MTR_8g106150 | 92.661 | 109 | 8 | 0 | 3 | 111 | 632 | 740 | 2.51e-66 | 217 |
| MS.gene001219.t1 | MTR_8g106150 | 92.661 | 109 | 8 | 0 | 3 | 111 | 875 | 983 | 1.17e-65 | 218 |
| MS.gene001219.t1 | MTR_8g106150 | 92.537 | 67 | 5 | 0 | 130 | 196 | 116 | 182 | 9.38e-33 | 124 |
| MS.gene001219.t1 | MTR_8g080770 | 64.815 | 108 | 31 | 1 | 3 | 110 | 855 | 955 | 7.19e-40 | 144 |
| MS.gene001219.t1 | MTR_8g080770 | 54.217 | 83 | 22 | 1 | 130 | 196 | 111 | 193 | 3.33e-19 | 85.1 |
| MS.gene001219.t1 | MTR_8g080770 | 64.815 | 108 | 31 | 1 | 3 | 110 | 746 | 846 | 1.35e-39 | 144 |
| MS.gene001219.t1 | MTR_8g080770 | 53.571 | 84 | 23 | 1 | 129 | 196 | 1 | 84 | 2.49e-19 | 85.5 |
| MS.gene001219.t1 | MTR_3g085130 | 62.963 | 108 | 32 | 1 | 3 | 110 | 920 | 1019 | 8.42e-38 | 138 |
| MS.gene001219.t1 | MTR_3g085130 | 55.422 | 83 | 21 | 1 | 130 | 196 | 166 | 248 | 2.16e-19 | 85.9 |
| MS.gene001219.t1 | MTR_3g085130 | 62.963 | 108 | 32 | 1 | 3 | 110 | 710 | 809 | 3.44e-37 | 137 |
| MS.gene001219.t1 | MTR_5g097410 | 58.261 | 115 | 43 | 1 | 2 | 111 | 1087 | 1201 | 7.69e-36 | 133 |
| MS.gene001219.t1 | MTR_5g097410 | 51.136 | 88 | 28 | 2 | 123 | 196 | 332 | 418 | 1.64e-18 | 83.2 |
| MS.gene001219.t1 | MTR_5g044100 | 73.333 | 60 | 16 | 0 | 1 | 60 | 164 | 223 | 2.14e-24 | 95.9 |
| MS.gene001219.t1 | MTR_8g075340 | 37.647 | 85 | 39 | 2 | 26 | 110 | 1084 | 1154 | 3.03e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001219.t1 | AT2G01830 | 69.725 | 109 | 26 | 1 | 3 | 111 | 948 | 1049 | 1.55e-44 | 158 |
| MS.gene001219.t1 | AT2G01830 | 76.471 | 68 | 15 | 1 | 130 | 196 | 193 | 260 | 5.05e-26 | 105 |
| MS.gene001219.t1 | AT2G01830 | 69.725 | 109 | 26 | 1 | 3 | 111 | 959 | 1060 | 1.71e-44 | 157 |
| MS.gene001219.t1 | AT2G01830 | 76.471 | 68 | 15 | 1 | 130 | 196 | 204 | 271 | 5.11e-26 | 105 |
| MS.gene001219.t1 | AT2G01830 | 69.725 | 109 | 26 | 1 | 3 | 111 | 936 | 1037 | 1.80e-44 | 157 |
| MS.gene001219.t1 | AT2G01830 | 76.471 | 68 | 15 | 1 | 130 | 196 | 181 | 248 | 5.50e-26 | 104 |
| MS.gene001219.t1 | AT2G01830 | 69.725 | 109 | 26 | 1 | 3 | 111 | 936 | 1037 | 1.80e-44 | 157 |
| MS.gene001219.t1 | AT2G01830 | 76.471 | 68 | 15 | 1 | 130 | 196 | 181 | 248 | 5.50e-26 | 104 |
| MS.gene001219.t1 | AT2G01830 | 69.725 | 109 | 26 | 1 | 3 | 111 | 936 | 1037 | 1.80e-44 | 157 |
| MS.gene001219.t1 | AT2G01830 | 76.471 | 68 | 15 | 1 | 130 | 196 | 181 | 248 | 5.50e-26 | 104 |
| MS.gene001219.t1 | AT2G01830 | 69.725 | 109 | 26 | 1 | 3 | 111 | 936 | 1037 | 1.80e-44 | 157 |
| MS.gene001219.t1 | AT2G01830 | 76.471 | 68 | 15 | 1 | 130 | 196 | 181 | 248 | 5.50e-26 | 104 |
| MS.gene001219.t1 | AT5G35750 | 64.103 | 117 | 37 | 2 | 3 | 114 | 1049 | 1165 | 5.19e-43 | 153 |
| MS.gene001219.t1 | AT5G35750 | 56.790 | 81 | 21 | 1 | 130 | 196 | 308 | 388 | 2.71e-19 | 85.5 |
| MS.gene001219.t1 | AT1G27320 | 65.217 | 115 | 35 | 2 | 1 | 110 | 902 | 1016 | 6.23e-43 | 153 |
| MS.gene001219.t1 | AT1G27320 | 53.012 | 83 | 23 | 1 | 130 | 196 | 169 | 251 | 1.31e-18 | 83.6 |
Find 0 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTAATTTTTTTTTTTTT+TGG | - | 43344:46181-46200 | MS.gene001219:CDS | 0.0% |
| !! | GATTTATAAAAAAACAATAT+GGG | + | 43344:46504-46523 | None:intergenic | 10.0% |
| !! | TGATTTATAAAAAAACAATA+TGG | + | 43344:46505-46524 | None:intergenic | 10.0% |
| !!! | ACTGTTAATTGAGTATTTTT+TGG | - | 43344:47092-47111 | MS.gene001219:intron | 20.0% |
| !!! | ATAAAAGTATCTGAGATTTT+AGG | - | 43344:47013-47032 | MS.gene001219:intron | 20.0% |
| !!! | CTGTTAATTGAGTATTTTTT+GGG | - | 43344:47093-47112 | MS.gene001219:intron | 20.0% |
| !!! | TTTCTGTGTAATTTTTGAAA+TGG | - | 43344:46886-46905 | MS.gene001219:CDS | 20.0% |
| ! | AACAAACATAACATGTATAG+AGG | + | 43344:46456-46475 | None:intergenic | 25.0% |
| ! | AATAAAGAGAAAAGTGACAA+AGG | + | 43344:46851-46870 | None:intergenic | 25.0% |
| ! | ACAAACATAACATGTATAGA+GGG | + | 43344:46455-46474 | None:intergenic | 25.0% |
| ! | TAAATTCCGTTTACCTATAT+TGG | - | 43344:46626-46645 | MS.gene001219:intron | 25.0% |
| ! | TATCATTGAATTTATCAGCA+AGG | + | 43344:46289-46308 | None:intergenic | 25.0% |
| !! | AGAAACTTTTGCAGAATATA+CGG | - | 43344:47251-47270 | MS.gene001219:intron | 25.0% |
| !! | CATGTACTTACATTATTTTC+AGG | - | 43344:46523-46542 | MS.gene001219:intron | 25.0% |
| !! | TGTATTAGTTAGTATTGAAC+TGG | - | 43344:46798-46817 | MS.gene001219:CDS | 25.0% |
| !!! | AGTTTTTTCTAAATCAGCAA+AGG | + | 43344:46233-46252 | None:intergenic | 25.0% |
| !!! | GCACGAACTTTTTTATATAT+AGG | + | 43344:47072-47091 | None:intergenic | 25.0% |
| !!! | TTGTTTTGTATGAGCATATA+TGG | - | 43344:46766-46785 | MS.gene001219:CDS | 25.0% |
| ATTCAAATGCAAGAAATGGA+CGG | - | 43344:46139-46158 | MS.gene001219:CDS | 30.0% | |
| GAAAGAGATTTAGTGTTAGT+TGG | - | 43344:47159-47178 | MS.gene001219:intron | 30.0% | |
| GGATATTCAAATGCAAGAAA+TGG | - | 43344:46135-46154 | MS.gene001219:CDS | 30.0% | |
| TAGAGTGGTTATAAAGACAA+TGG | - | 43344:47365-47384 | MS.gene001219:CDS | 30.0% | |
| TATAAAGACAATGGAAAGAG+AGG | - | 43344:47374-47393 | MS.gene001219:CDS | 30.0% | |
| TGAACTCTTCTTCATATCTA+TGG | + | 43344:47123-47142 | None:intergenic | 30.0% | |
| ! | ATTAGTTAGTATTGAACTGG+TGG | - | 43344:46801-46820 | MS.gene001219:CDS | 30.0% |
| ! | TTATGTCTCAAAACCTTTTG+AGG | - | 43344:46710-46729 | MS.gene001219:intron | 30.0% |
| !! | ATGAAGAGTGTTTGAAATGT+GGG | - | 43344:46679-46698 | MS.gene001219:intron | 30.0% |
| !! | TATGAAGAGTGTTTGAAATG+TGG | - | 43344:46678-46697 | MS.gene001219:intron | 30.0% |
| !!! | TAGTCTAAGATGTTTTGGTT+TGG | - | 43344:46912-46931 | MS.gene001219:CDS | 30.0% |
| AACACTCTTCATATGTAGCA+TGG | + | 43344:46671-46690 | None:intergenic | 35.0% | |
| GAAGAAGAGTTCAGAGAAAT+AGG | - | 43344:47130-47149 | MS.gene001219:intron | 35.0% | |
| GTAAACAACTCTTTGTGCAT+AGG | + | 43344:47316-47335 | None:intergenic | 35.0% | |
| TCATGGCCAATATAGGTAAA+CGG | + | 43344:46635-46654 | None:intergenic | 35.0% | |
| TGAACAATTCCTTCCTCAAA+AGG | + | 43344:46726-46745 | None:intergenic | 35.0% | |
| TGCACAAAGAGTTGTTTACT+CGG | - | 43344:47317-47336 | MS.gene001219:CDS | 35.0% | |
| TGCTGTAATGTTACTGTACT+TGG | - | 43344:46954-46973 | MS.gene001219:intron | 35.0% | |
| TTACGTGCATTCAATTTACG+TGG | + | 43344:46313-46332 | None:intergenic | 35.0% | |
| TTCAAATGCAAGAAATGGAC+GGG | - | 43344:46140-46159 | MS.gene001219:CDS | 35.0% | |
| ! | TCTCAGATACTTTTATCTGC+TGG | + | 43344:47009-47028 | None:intergenic | 35.0% |
| !! | AGTGTTAGTTGGTGTTGTAA+TGG | - | 43344:47170-47189 | MS.gene001219:intron | 35.0% |
| !! | CTTGTAACTTTGAAGCCAAA+GGG | + | 43344:47233-47252 | None:intergenic | 35.0% |
| !! | GGTCTTAGTCTAAGATGTTT+TGG | - | 43344:46907-46926 | MS.gene001219:CDS | 35.0% |
| !! | GTGTTAGTTGGTGTTGTAAT+GGG | - | 43344:47171-47190 | MS.gene001219:intron | 35.0% |
| !! | TCTTGTAACTTTGAAGCCAA+AGG | + | 43344:47234-47253 | None:intergenic | 35.0% |
| !! | TTTTGAGGAAGGAATTGTTC+AGG | - | 43344:46725-46744 | MS.gene001219:intron | 35.0% |
| AAGCAAGCATCAAACTTGTG+AGG | + | 43344:46112-46131 | None:intergenic | 40.0% | |
| AGCAAGCATCAAACTTGTGA+GGG | + | 43344:46111-46130 | None:intergenic | 40.0% | |
| CAAGTTTGATGCTTGCTTCA+AGG | - | 43344:46114-46133 | MS.gene001219:CDS | 40.0% | |
| CGAATGGTGAATGTAGTGAA+AGG | - | 43344:46598-46617 | MS.gene001219:intron | 40.0% | |
| GTTTCCTGAGCAAATATGAC+CGG | + | 43344:47426-47445 | None:intergenic | 40.0% | |
| TTGAAGCAACTCGTCGAATT+CGG | - | 43344:46547-46566 | MS.gene001219:intron | 40.0% | |
| TTGTTTACTCGGAAAGAGAG+CGG | - | 43344:47328-47347 | MS.gene001219:CDS | 40.0% | |
| ! | AATGCACGTAACTTGCATGT+TGG | - | 43344:46322-46341 | MS.gene001219:intron | 40.0% |
| ! | AGAGTGTTTGAAATGTGGGA+TGG | - | 43344:46683-46702 | MS.gene001219:intron | 40.0% |
| ! | ATCCGCTCCAAAGTTTTTCA+AGG | + | 43344:46041-46060 | None:intergenic | 40.0% |
| ! | ATGGATTACATCTGCTGTCA+TGG | + | 43344:46652-46671 | None:intergenic | 40.0% |
| ! | CTTTTGCAGAATATACGGCT+AGG | - | 43344:47256-47275 | MS.gene001219:intron | 40.0% |
| ! | TGTTTGAAATGTGGGATGGA+TGG | - | 43344:46687-46706 | MS.gene001219:intron | 40.0% |
| !! | GTCTCAAAACCTTTTGAGGA+AGG | - | 43344:46714-46733 | MS.gene001219:intron | 40.0% |
| AAAGAGAGGCTTCACAGGTT+AGG | - | 43344:47388-47407 | MS.gene001219:CDS | 45.0% | |
| AAGAGAGGCTTCACAGGTTA+GGG | - | 43344:47389-47408 | MS.gene001219:CDS | 45.0% | |
| AATGGAAAGAGAGGCTTCAC+AGG | - | 43344:47383-47402 | MS.gene001219:CDS | 45.0% | |
| GTTTGAGAAGCAGCATAGAG+TGG | - | 43344:47350-47369 | MS.gene001219:CDS | 45.0% | |
| TCTGCTGTCATGGCCAATAT+AGG | + | 43344:46642-46661 | None:intergenic | 45.0% | |
| TGAAGCAACTCGTCGAATTC+GGG | - | 43344:46548-46567 | MS.gene001219:intron | 45.0% | |
| ! | CGCCTTGAAAAACTTTGGAG+CGG | - | 43344:46036-46055 | MS.gene001219:CDS | 45.0% |
| !! | GTAACTTTGAAGCCAAAGGG+TGG | + | 43344:47230-47249 | None:intergenic | 45.0% |
| AACTCGTCGAATTCGGGAGA+TGG | - | 43344:46554-46573 | MS.gene001219:intron | 50.0% | |
| AATTCGGGAGATGGAGAGGA+CGG | - | 43344:46563-46582 | MS.gene001219:intron | 50.0% | |
| GATGTGAAGTGTGCAGACAG+CGG | - | 43344:46058-46077 | MS.gene001219:CDS | 50.0% | |
| GCAAAATCAGCACCACCCTT+TGG | - | 43344:47215-47234 | MS.gene001219:intron | 50.0% | |
| GCAGGCGCCTTGAAAAACTT+TGG | - | 43344:46031-46050 | MS.gene001219:CDS | 50.0% | |
| GGTTAGGGATGAGTATGCAC+CGG | - | 43344:47404-47423 | MS.gene001219:CDS | 50.0% | |
| ! | TGCACCGGTCATATTTGCTC+AGG | - | 43344:47419-47438 | MS.gene001219:CDS | 50.0% |
| ACGGCGAATGAGGAGACGAA+TGG | - | 43344:46582-46601 | MS.gene001219:intron | 55.0% | |
| GTCGAATTCGGGAGATGGAG+AGG | - | 43344:46559-46578 | MS.gene001219:intron | 55.0% | |
| TGTGCATAGGCCACTCCACT+CGG | + | 43344:47303-47322 | None:intergenic | 55.0% | |
| CGGCTAGGACCGCTTTCGAA+AGG | - | 43344:47271-47290 | MS.gene001219:intron | 60.0% | |
| GATGGAGAGGACGGCGAATG+AGG | - | 43344:46572-46591 | MS.gene001219:intron | 60.0% | |
| TAGGCCACTCCACTCGGTAG+CGG | + | 43344:47297-47316 | None:intergenic | 60.0% | |
| TTCGAAAGGCCGCTACCGAG+TGG | - | 43344:47285-47304 | MS.gene001219:intron | 60.0% | |
| ! | CGGTAGCGGCCTTTCGAAAG+CGG | + | 43344:47283-47302 | None:intergenic | 60.0% |
| AAGGCCGCTACCGAGTGGAG+TGG | - | 43344:47290-47309 | MS.gene001219:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43344 | gene | 46019 | 47458 | 46019 | ID=MS.gene001219 |
| 43344 | mRNA | 46019 | 47458 | 46019 | ID=MS.gene001219.t1;Parent=MS.gene001219 |
| 43344 | exon | 47316 | 47458 | 47316 | ID=MS.gene001219.t1.exon1;Parent=MS.gene001219.t1 |
| 43344 | CDS | 47316 | 47458 | 47316 | ID=cds.MS.gene001219.t1;Parent=MS.gene001219.t1 |
| 43344 | exon | 46731 | 46932 | 46731 | ID=MS.gene001219.t1.exon2;Parent=MS.gene001219.t1 |
| 43344 | CDS | 46731 | 46932 | 46731 | ID=cds.MS.gene001219.t1;Parent=MS.gene001219.t1 |
| 43344 | exon | 46019 | 46270 | 46019 | ID=MS.gene001219.t1.exon3;Parent=MS.gene001219.t1 |
| 43344 | CDS | 46019 | 46270 | 46019 | ID=cds.MS.gene001219.t1;Parent=MS.gene001219.t1 |
| Gene Sequence |
| Protein sequence |
