Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001507.t1 | XP_024632961.1 | 94.5 | 145 | 8 | 0 | 1 | 145 | 81 | 225 | 1.90E-73 | 285 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001507.t1 | G7I8T1 | 93.8 | 145 | 9 | 0 | 1 | 145 | 81 | 225 | 1.8e-73 | 284.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene001507.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001507.t1 | MTR_1g083640 | 93.793 | 145 | 9 | 0 | 1 | 145 | 81 | 225 | 1.12e-100 | 290 |
| MS.gene001507.t1 | MTR_0558s0020 | 93.103 | 145 | 10 | 0 | 1 | 145 | 66 | 210 | 2.52e-98 | 285 |
| MS.gene001507.t1 | MTR_2g014100 | 90.345 | 145 | 14 | 0 | 1 | 145 | 74 | 218 | 7.37e-96 | 277 |
| MS.gene001507.t1 | MTR_6g090505 | 89.655 | 145 | 15 | 0 | 1 | 145 | 1 | 145 | 5.69e-93 | 273 |
| MS.gene001507.t1 | MTR_3g008240 | 92.754 | 138 | 10 | 0 | 1 | 138 | 66 | 203 | 2.17e-91 | 264 |
| MS.gene001507.t1 | MTR_4g040430 | 90.580 | 138 | 9 | 1 | 1 | 138 | 65 | 198 | 5.66e-88 | 255 |
| MS.gene001507.t1 | MTR_0337s0040 | 78.873 | 142 | 30 | 0 | 4 | 145 | 30 | 171 | 9.06e-81 | 238 |
| MS.gene001507.t1 | MTR_1g055240 | 92.500 | 120 | 9 | 0 | 26 | 145 | 2 | 121 | 1.34e-80 | 234 |
| MS.gene001507.t1 | MTR_0019s0290 | 86.719 | 128 | 17 | 0 | 18 | 145 | 9 | 136 | 3.57e-80 | 234 |
| MS.gene001507.t1 | MTR_8g446020 | 78.169 | 142 | 31 | 0 | 4 | 145 | 29 | 170 | 1.34e-77 | 231 |
| MS.gene001507.t1 | MTR_5g071590 | 88.462 | 104 | 12 | 0 | 1 | 104 | 78 | 181 | 3.40e-66 | 199 |
| MS.gene001507.t1 | MTR_1281s0010 | 93.651 | 63 | 4 | 0 | 1 | 63 | 47 | 109 | 3.46e-36 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 25 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTGATGTTGTCCTGTTGTT+AGG | 0.288908 | 1.4:+52928696 | None:intergenic |
| TTACTTCTCGAAATGATCTA+AGG | 0.311482 | 1.4:+52928505 | None:intergenic |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 1.4:-52928430 | MS.gene001507:CDS |
| ATGTGTATAAGATATATAGA+TGG | 0.407680 | 1.4:-52928575 | MS.gene001507:CDS |
| GGCACGAAGAATCTTCCTTT+CGG | 0.417723 | 1.4:-52928393 | MS.gene001507:CDS |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | 1.4:-52928417 | MS.gene001507:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 1.4:-52928492 | MS.gene001507:CDS |
| ACAAAATAAGAAATTTGATA+AGG | 0.431852 | 1.4:-52928778 | MS.gene001507:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.447509 | 1.4:-52928627 | MS.gene001507:CDS |
| GCTCTTATAAATTCTAAAGC+TGG | 0.462891 | 1.4:-52928462 | MS.gene001507:CDS |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 1.4:-52928429 | MS.gene001507:CDS |
| GTTTGCTTAATCCTAACAAC+AGG | 0.467124 | 1.4:-52928707 | MS.gene001507:CDS |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 1.4:-52928744 | MS.gene001507:CDS |
| AGCTGGAATATCTTTGAAGC+TGG | 0.496270 | 1.4:-52928610 | MS.gene001507:CDS |
| CTAAGGTAACATGAATGCTC+TGG | 0.504903 | 1.4:+52928522 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 1.4:-52928733 | MS.gene001507:CDS |
| GAATGCTCTGGTTTATGTAA+TGG | 0.517705 | 1.4:+52928534 | None:intergenic |
| TTACAACCAACCCTTTCCTC+CGG | 0.518116 | 1.4:+52928633 | None:intergenic |
| TGTGTATAAGATATATAGAT+GGG | 0.523615 | 1.4:-52928574 | MS.gene001507:CDS |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 1.4:-52928732 | MS.gene001507:CDS |
| TTCACAAACTCAATAACCAA+TGG | 0.554620 | 1.4:+52928669 | None:intergenic |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 1.4:-52928414 | MS.gene001507:CDS |
| TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | 1.4:-52928423 | MS.gene001507:CDS |
| GACAACATCAGACAGTCCAT+TGG | 0.604607 | 1.4:-52928685 | MS.gene001507:CDS |
| CTTTAAGTCTGAAAACCGAA+AGG | 0.617766 | 1.4:+52928378 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATTTGATA+AGG | - | chr1.4:52928403-52928422 | MS.gene001507:CDS | 15.0% |
| !! | ATGTGTATAAGATATATAGA+TGG | - | chr1.4:52928606-52928625 | MS.gene001507:CDS | 20.0% |
| !! | TGTGTATAAGATATATAGAT+GGG | - | chr1.4:52928607-52928626 | MS.gene001507:CDS | 20.0% |
| AACAAACAAGGTTTCAAGAA+GGG | - | chr1.4:52928449-52928468 | MS.gene001507:CDS | 30.0% | |
| AGAACGTCTTATGAAGTTAT+GGG | - | chr1.4:52928752-52928771 | MS.gene001507:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | - | chr1.4:52928437-52928456 | MS.gene001507:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | - | chr1.4:52928448-52928467 | MS.gene001507:CDS | 30.0% | |
| TGTGAAAGTGAAAGAAAAAC+CGG | - | chr1.4:52928529-52928548 | MS.gene001507:CDS | 30.0% | |
| TTACTTCTCGAAATGATCTA+AGG | + | chr1.4:52928679-52928698 | None:intergenic | 30.0% | |
| TTCACAAACTCAATAACCAA+TGG | + | chr1.4:52928515-52928534 | None:intergenic | 30.0% | |
| !! | GCTCTTATAAATTCTAAAGC+TGG | - | chr1.4:52928719-52928738 | MS.gene001507:CDS | 30.0% |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr1.4:52928689-52928708 | MS.gene001507:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | - | chr1.4:52928751-52928770 | MS.gene001507:CDS | 35.0% | |
| GTTTGCTTAATCCTAACAAC+AGG | - | chr1.4:52928474-52928493 | MS.gene001507:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | - | chr1.4:52928758-52928777 | MS.gene001507:CDS | 35.0% | |
| ! | GAATGCTCTGGTTTATGTAA+TGG | + | chr1.4:52928650-52928669 | None:intergenic | 35.0% |
| CTAAGGTAACATGAATGCTC+TGG | + | chr1.4:52928662-52928681 | None:intergenic | 40.0% | |
| GAAAGAAAAACCGGAGGAAA+GGG | - | chr1.4:52928538-52928557 | MS.gene001507:CDS | 40.0% | |
| GAAAGTGAAAGAAAAACCGG+AGG | - | chr1.4:52928532-52928551 | MS.gene001507:CDS | 40.0% | |
| TCTGATGTTGTCCTGTTGTT+AGG | + | chr1.4:52928488-52928507 | None:intergenic | 40.0% | |
| TGAAAGAAAAACCGGAGGAA+AGG | - | chr1.4:52928537-52928556 | MS.gene001507:CDS | 40.0% | |
| !! | AGCTGGAATATCTTTGAAGC+TGG | - | chr1.4:52928571-52928590 | MS.gene001507:CDS | 40.0% |
| GAAGTTATGGGTCAAGGAGT+TGG | - | chr1.4:52928764-52928783 | MS.gene001507:CDS | 45.0% | |
| GACAACATCAGACAGTCCAT+TGG | - | chr1.4:52928496-52928515 | MS.gene001507:CDS | 45.0% | |
| GGCACGAAGAATCTTCCTTT+CGG | - | chr1.4:52928788-52928807 | MS.gene001507:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+CGG | + | chr1.4:52928551-52928570 | None:intergenic | 45.0% | |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr1.4:52928554-52928573 | MS.gene001507:CDS | 45.0% |
| GAAAAACCGGAGGAAAGGGT+TGG | - | chr1.4:52928542-52928561 | MS.gene001507:CDS | 50.0% | |
| GTTATGGGTCAAGGAGTTGG+AGG | - | chr1.4:52928767-52928786 | MS.gene001507:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 52928380 | 52928823 | 52928380 | ID=MS.gene001507 |
| chr1.4 | mRNA | 52928380 | 52928823 | 52928380 | ID=MS.gene001507.t1;Parent=MS.gene001507 |
| chr1.4 | exon | 52928380 | 52928823 | 52928380 | ID=MS.gene001507.t1.exon1;Parent=MS.gene001507.t1 |
| chr1.4 | CDS | 52928380 | 52928823 | 52928380 | ID=cds.MS.gene001507.t1;Parent=MS.gene001507.t1 |
| Gene Sequence |
| Protein sequence |
