Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001508.t1 | XP_024632961.1 | 95.2 | 310 | 15 | 0 | 1 | 310 | 1 | 310 | 3.40E-152 | 547.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001508.t1 | Q9SZL8 | 28.8 | 257 | 165 | 6 | 52 | 308 | 74 | 312 | 3.5e-25 | 117.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001508.t1 | A0A072TE13 | 86.5 | 310 | 27 | 1 | 1 | 310 | 1 | 295 | 3.6e-148 | 533.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene001508.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001508.t1 | MTR_0558s0020 | 88.710 | 310 | 20 | 1 | 1 | 310 | 1 | 295 | 0.0 | 571 |
| MS.gene001508.t1 | MTR_1g083640 | 94.406 | 286 | 16 | 0 | 1 | 286 | 1 | 286 | 0.0 | 565 |
| MS.gene001508.t1 | MTR_8g446020 | 76.452 | 310 | 18 | 3 | 1 | 310 | 1 | 255 | 1.94e-166 | 462 |
| MS.gene001508.t1 | MTR_2g014100 | 86.561 | 253 | 25 | 2 | 1 | 252 | 1 | 245 | 5.29e-159 | 444 |
| MS.gene001508.t1 | MTR_6g090505 | 91.304 | 230 | 20 | 0 | 81 | 310 | 1 | 230 | 1.95e-157 | 444 |
| MS.gene001508.t1 | MTR_0337s0040 | 73.913 | 276 | 18 | 2 | 1 | 276 | 1 | 222 | 1.49e-139 | 394 |
| MS.gene001508.t1 | MTR_3g008240 | 84.404 | 218 | 19 | 1 | 1 | 218 | 1 | 203 | 3.37e-131 | 371 |
| MS.gene001508.t1 | MTR_4g040430 | 84.404 | 218 | 14 | 3 | 1 | 218 | 1 | 198 | 3.52e-128 | 363 |
| MS.gene001508.t1 | MTR_5g071590 | 87.432 | 183 | 20 | 1 | 1 | 183 | 1 | 180 | 9.60e-117 | 334 |
| MS.gene001508.t1 | MTR_0019s0290 | 89.474 | 152 | 16 | 0 | 98 | 249 | 9 | 160 | 2.57e-98 | 286 |
| MS.gene001508.t1 | MTR_1g055240 | 93.706 | 143 | 9 | 0 | 106 | 248 | 2 | 144 | 7.95e-98 | 285 |
| MS.gene001508.t1 | MTR_1281s0010 | 92.661 | 109 | 8 | 0 | 35 | 143 | 1 | 109 | 2.28e-69 | 211 |
| MS.gene001508.t1 | MTR_8g046060 | 77.083 | 96 | 13 | 2 | 1 | 95 | 91 | 178 | 2.53e-37 | 132 |
| MS.gene001508.t1 | MTR_4g008820 | 74.737 | 95 | 9 | 1 | 1 | 95 | 125 | 204 | 8.57e-36 | 136 |
| MS.gene001508.t1 | MTR_6g051425 | 31.250 | 256 | 147 | 6 | 56 | 304 | 22 | 255 | 1.08e-30 | 118 |
| MS.gene001508.t1 | MTR_1g070355 | 28.458 | 253 | 158 | 6 | 57 | 307 | 80 | 311 | 8.03e-22 | 95.9 |
| MS.gene001508.t1 | MTR_0337s0050 | 83.333 | 36 | 6 | 0 | 275 | 310 | 14 | 49 | 1.06e-15 | 71.2 |
| MS.gene001508.t1 | MTR_1g013060 | 29.032 | 217 | 133 | 8 | 56 | 269 | 29 | 227 | 1.54e-13 | 69.3 |
| MS.gene001508.t1 | MTR_0402s0040 | 27.419 | 186 | 114 | 5 | 65 | 249 | 40 | 205 | 2.15e-13 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001508.t1 | AT4G38180 | 29.008 | 262 | 158 | 7 | 52 | 308 | 74 | 312 | 2.28e-26 | 109 |
| MS.gene001508.t1 | AT5G28530 | 25.769 | 260 | 163 | 7 | 56 | 303 | 60 | 301 | 2.62e-15 | 77.0 |
| MS.gene001508.t1 | AT5G28530 | 25.769 | 260 | 163 | 7 | 56 | 303 | 60 | 301 | 2.62e-15 | 77.0 |
| MS.gene001508.t1 | AT5G28530 | 25.769 | 260 | 163 | 7 | 56 | 303 | 60 | 301 | 2.62e-15 | 77.0 |
| MS.gene001508.t1 | AT1G10240 | 22.556 | 266 | 186 | 7 | 48 | 310 | 45 | 293 | 8.38e-13 | 69.3 |
| MS.gene001508.t1 | AT1G10240 | 22.556 | 266 | 186 | 7 | 48 | 310 | 45 | 293 | 8.38e-13 | 69.3 |
Find 0 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATTTGATA+AGG | - | 8724:683-702 | MS.gene001508:CDS | 15.0% |
| !! | ATGTGTATAAGATATATAGA+TGG | - | 8724:886-905 | MS.gene001508:CDS | 20.0% |
| !! | TGTGTATAAGATATATAGAT+GGG | - | 8724:887-906 | MS.gene001508:CDS | 20.0% |
| !!! | AAAGAGATTGAAGATTTTTA+TGG | - | 8724:600-619 | MS.gene001508:CDS | 20.0% |
| !!! | ATAAAACTGGATTTTCTATT+AGG | - | 8724:634-653 | MS.gene001508:CDS | 20.0% |
| ! | ACCGAACAAACAATAAATAT+CGG | - | 8724:1321-1340 | MS.gene001508:CDS | 25.0% |
| ! | CAACTAGATTATTCTCTATT+CGG | - | 8724:1272-1291 | MS.gene001508:CDS | 25.0% |
| ! | GGTAATTATGCATATAAAAC+TGG | - | 8724:621-640 | MS.gene001508:CDS | 25.0% |
| ! | TGGATATCATAGTAAAAAGA+TGG | + | 8724:1200-1219 | MS.gene001508:intergenic | 25.0% |
| !!! | AAGACATAGCTAGTATTTTT+TGG | - | 8724:1234-1253 | MS.gene001508:CDS | 25.0% |
| !!! | AGACATAGCTAGTATTTTTT+GGG | - | 8724:1235-1254 | MS.gene001508:CDS | 25.0% |
| AACAAACAAGGTTTCAAGAA+GGG | - | 8724:729-748 | MS.gene001508:CDS | 30.0% | |
| AGAACGTCTTATGAAGTTAT+GGG | - | 8724:1032-1051 | MS.gene001508:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | - | 8724:717-736 | MS.gene001508:CDS | 30.0% | |
| GCCGATATTTATTGTTTGTT+CGG | + | 8724:1325-1344 | MS.gene001508:intergenic | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | - | 8724:728-747 | MS.gene001508:CDS | 30.0% | |
| TCCATGATACTTTATTGCTA+GGG | + | 8724:557-576 | MS.gene001508:intergenic | 30.0% | |
| TGTGAAAGTGAAAGAAAAAC+CGG | - | 8724:809-828 | MS.gene001508:CDS | 30.0% | |
| TTACTTCTCGAAATGATCTA+AGG | + | 8724:959-978 | MS.gene001508:intergenic | 30.0% | |
| TTCACAAACTCAATAACCAA+TGG | + | 8724:795-814 | MS.gene001508:intergenic | 30.0% | |
| TTCCATGATACTTTATTGCT+AGG | + | 8724:558-577 | MS.gene001508:intergenic | 30.0% | |
| TTTAAGTCTGAAAACCGAAA+AGG | + | 8724:1086-1105 | MS.gene001508:intergenic | 30.0% | |
| !! | GCTCTTATAAATTCTAAAGC+TGG | - | 8724:999-1018 | MS.gene001508:CDS | 30.0% |
| AATTCGTCAAAAAGAGATGG+TGG | - | 8724:1121-1140 | MS.gene001508:CDS | 35.0% | |
| ATATCAACTTCATCTGAGAG+TGG | - | 8724:429-448 | MS.gene001508:CDS | 35.0% | |
| CCATGATACTTTATTGCTAG+GGG | + | 8724:556-575 | MS.gene001508:intergenic | 35.0% | |
| CGAGAAGTAAATGAAGTTCA+AGG | - | 8724:969-988 | MS.gene001508:CDS | 35.0% | |
| GACAATTCGTCAAAAAGAGA+TGG | - | 8724:1118-1137 | MS.gene001508:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | - | 8724:1031-1050 | MS.gene001508:CDS | 35.0% | |
| GGAGTAACATTGAGAACTAT+CGG | + | 8724:535-554 | MS.gene001508:intergenic | 35.0% | |
| GTTTGCTTAATCCTAACAAC+AGG | - | 8724:754-773 | MS.gene001508:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | - | 8724:1038-1057 | MS.gene001508:CDS | 35.0% | |
| ! | GAATGCTCTGGTTTATGTAA+TGG | + | 8724:930-949 | MS.gene001508:intergenic | 35.0% |
| CTAAGGTAACATGAATGCTC+TGG | + | 8724:942-961 | MS.gene001508:intergenic | 40.0% | |
| GAAAGAAAAACCGGAGGAAA+GGG | - | 8724:818-837 | MS.gene001508:CDS | 40.0% | |
| GAAAGTGAAAGAAAAACCGG+AGG | - | 8724:812-831 | MS.gene001508:CDS | 40.0% | |
| TCTGATGTTGTCCTGTTGTT+AGG | + | 8724:768-787 | MS.gene001508:intergenic | 40.0% | |
| TGAAAGAAAAACCGGAGGAA+AGG | - | 8724:817-836 | MS.gene001508:CDS | 40.0% | |
| TGTCTTCTGTAGCATCAACT+TGG | + | 8724:1220-1239 | MS.gene001508:intergenic | 40.0% | |
| ! | CCCCTAGCAATAAAGTATCA+TGG | - | 8724:553-572 | MS.gene001508:CDS | 40.0% |
| ! | GCACGAAGAATCTTCCTTTT+CGG | - | 8724:1069-1088 | MS.gene001508:CDS | 40.0% |
| !! | AGCTGGAATATCTTTGAAGC+TGG | - | 8724:851-870 | MS.gene001508:CDS | 40.0% |
| !! | TTACTGATTCATGCTGAGAG+TGG | - | 8724:474-493 | MS.gene001508:CDS | 40.0% |
| !!! | GCTAGTATTTTTTGGGCAGA+TGG | - | 8724:1242-1261 | MS.gene001508:CDS | 40.0% |
| GAAGTTATGGGTCAAGGAGT+TGG | - | 8724:1044-1063 | MS.gene001508:CDS | 45.0% | |
| GACAACATCAGACAGTCCAT+TGG | - | 8724:776-795 | MS.gene001508:CDS | 45.0% | |
| TTACAACCAACCCTTTCCTC+CGG | + | 8724:831-850 | MS.gene001508:intergenic | 45.0% | |
| ! | CGTCAAAAAGAGATGGTGGT+TGG | - | 8724:1125-1144 | MS.gene001508:CDS | 45.0% |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | 8724:834-853 | MS.gene001508:CDS | 45.0% |
| GAAAAACCGGAGGAAAGGGT+TGG | - | 8724:822-841 | MS.gene001508:CDS | 50.0% | |
| GTTATGGGTCAAGGAGTTGG+AGG | - | 8724:1047-1066 | MS.gene001508:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 8724 | gene | 420 | 1350 | 420 | ID=MS.gene001508 |
| 8724 | mRNA | 420 | 1350 | 420 | ID=MS.gene001508.t1;Parent=MS.gene001508 |
| 8724 | exon | 420 | 1350 | 420 | ID=MS.gene001508.t1.exon1;Parent=MS.gene001508.t1 |
| 8724 | CDS | 420 | 1350 | 420 | ID=cds.MS.gene001508.t1;Parent=MS.gene001508.t1 |
| Gene Sequence |
| Protein sequence |
