AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene001530

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001530.t1 MTR_2g017890 78.910 422 49 2 1 422 2 383 0.0 641
MS.gene001530.t1 MTR_8g087540 48.000 325 163 3 1 324 1 320 1.39e-99 303
MS.gene001530.t1 MTR_5g029680 45.405 185 97 2 13 193 31 215 2.54e-52 183
MS.gene001530.t1 MTR_2g043940 51.786 168 81 0 66 233 14 181 2.09e-51 177
MS.gene001530.t1 MTR_4g077970 40.741 189 101 2 12 193 10 194 4.54e-47 168
MS.gene001530.t1 MTR_7g095680 41.989 181 105 0 13 193 48 228 1.50e-44 159
MS.gene001530.t1 MTR_3g104550 40.113 177 103 1 13 189 45 218 1.15e-43 157
MS.gene001530.t1 MTR_2g100680 35.424 271 143 7 6 257 23 280 1.79e-43 158
MS.gene001530.t1 MTR_2g100670 41.206 199 104 3 1 193 14 205 5.97e-43 153
MS.gene001530.t1 MTR_6g043060 61.111 108 42 0 4 111 12 119 1.11e-42 153
MS.gene001530.t1 MTR_2g100670 41.414 198 103 3 1 192 122 312 2.87e-42 154
MS.gene001530.t1 MTR_1g102860 38.830 188 111 1 5 192 31 214 1.21e-41 150
MS.gene001530.t1 MTR_8g105780 44.624 186 99 2 12 193 20 205 2.66e-41 150
MS.gene001530.t1 MTR_1g061170 37.569 181 108 1 12 192 47 222 3.14e-41 152
MS.gene001530.t1 MTR_8g083100 40.930 215 92 7 15 213 23 218 7.65e-41 151
MS.gene001530.t1 MTR_5g017470 42.775 173 69 2 14 156 9 181 3.30e-40 145
MS.gene001530.t1 MTR_6g086805 39.779 181 102 2 12 192 9 182 2.69e-39 144
MS.gene001530.t1 MTR_1g071360 38.298 188 102 2 5 192 23 196 7.14e-39 143
MS.gene001530.t1 MTR_1g071360 38.298 188 102 2 5 192 20 193 1.68e-38 143
MS.gene001530.t1 MTR_5g010680 57.009 107 45 1 2 107 11 117 2.32e-37 139
MS.gene001530.t1 MTR_3g101870 61.000 100 38 1 8 106 17 116 9.97e-36 135
MS.gene001530.t1 MTR_1g100777 54.717 106 47 1 3 107 6 111 6.96e-35 131
MS.gene001530.t1 MTR_5g089170 57.000 100 42 1 8 106 18 117 3.67e-33 125
MS.gene001530.t1 MTR_7g091370 47.619 105 55 0 14 118 22 126 6.11e-32 121
MS.gene001530.t1 MTR_0020s0020 56.522 92 40 0 14 105 33 124 1.11e-31 124
MS.gene001530.t1 MTR_4g088900 38.037 163 85 3 14 164 33 191 3.77e-27 108
MS.gene001530.t1 MTR_4g045760 51.724 116 39 2 289 404 3 101 5.98e-26 101
MS.gene001530.t1 MTR_4g045757 64.789 71 25 0 1 71 1 71 1.89e-25 99.4
MS.gene001530.t1 MTR_5g047190 49.485 97 42 2 22 111 1 97 7.50e-25 98.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001530.t1 AT4G18880 43.963 323 166 7 2 315 3 319 6.34e-70 226
MS.gene001530.t1 AT5G45710 40.248 323 152 5 1 323 1 282 2.07e-63 207
MS.gene001530.t1 AT5G45710 40.248 323 152 5 1 323 1 282 2.07e-63 207
MS.gene001530.t1 AT4G17750 43.979 191 96 1 13 192 51 241 2.02e-50 177
MS.gene001530.t1 AT1G32330 42.408 191 104 2 8 192 31 221 2.33e-47 169
MS.gene001530.t1 AT1G32330 42.408 191 104 2 8 192 31 221 2.49e-47 169
MS.gene001530.t1 AT2G26150 41.667 180 102 1 13 192 43 219 2.34e-46 163
MS.gene001530.t1 AT2G26150 41.667 180 102 1 13 192 43 219 2.34e-46 163
MS.gene001530.t1 AT3G02990 41.530 183 106 1 12 193 21 203 5.74e-46 165
MS.gene001530.t1 AT4G13980 40.196 204 108 3 15 212 24 219 4.07e-45 162
MS.gene001530.t1 AT5G16820 40.323 186 109 1 10 193 23 208 8.69e-45 162
MS.gene001530.t1 AT5G16820 40.323 186 109 1 10 193 23 208 8.69e-45 162
MS.gene001530.t1 AT5G03720 38.378 185 103 2 12 193 53 229 4.70e-43 156
MS.gene001530.t1 AT5G03720 38.378 185 103 2 12 193 53 229 4.70e-43 156
MS.gene001530.t1 AT3G22830 40.217 184 106 1 13 192 60 243 1.08e-42 155
MS.gene001530.t1 AT5G62020 51.128 133 57 2 1 132 9 134 2.17e-39 143
MS.gene001530.t1 AT3G24520 38.674 181 85 2 12 192 15 169 2.61e-39 144
MS.gene001530.t1 AT1G67970 40.659 182 104 3 12 192 17 195 3.29e-39 145
MS.gene001530.t1 AT5G43840 41.848 184 88 3 11 192 14 180 4.40e-39 142
MS.gene001530.t1 AT3G63350 39.247 186 92 3 14 192 28 199 5.44e-38 139
MS.gene001530.t1 AT3G51910 35.638 188 103 2 5 192 20 189 1.91e-37 137
MS.gene001530.t1 AT1G46264 59.813 107 39 2 1 106 23 126 5.59e-37 138
MS.gene001530.t1 AT4G36990 57.576 99 42 0 14 112 14 112 1.26e-34 130
MS.gene001530.t1 AT5G54070 37.017 181 98 3 12 190 69 235 2.17e-34 130
MS.gene001530.t1 AT2G41690 53.398 103 47 1 13 114 39 141 3.90e-34 127
MS.gene001530.t1 AT4G11660 58.696 92 38 0 14 105 59 150 1.38e-33 129
MS.gene001530.t1 AT5G45710 32.301 226 113 4 98 323 1 186 1.31e-22 96.7
MS.gene001530.t1 AT5G45710 32.301 226 113 4 98 323 1 186 1.31e-22 96.7
MS.gene001530.t1 AT4G18870 48.864 88 45 0 14 101 146 233 7.04e-22 95.5
MS.gene001530.t1 AT4G18870 46.535 101 52 2 1 101 1 99 1.33e-21 94.7
MS.gene001530.t1 AT1G77570 37.778 90 53 2 15 101 27 116 2.73e-15 73.2
MS.gene001530.t1 AT2G26150 29.412 119 81 1 74 192 49 164 5.66e-14 72.4
MS.gene001530.t1 AT2G26150 29.412 119 81 1 74 192 49 164 5.66e-14 72.4

Find 81 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGATTATAATAATGAATTT+TGG 0.100021 2.2:-63862001 MS.gene001530:CDS
GTTACGACGGGTTGTTCTTC+TGG 0.216236 2.2:+63862038 None:intergenic
CAACTGATGACATTGTTTCT+TGG 0.248061 2.2:-63863687 MS.gene001530:CDS
AGTTGCATACAAGATGTTAT+TGG 0.278275 2.2:+63862296 None:intergenic
AACTGATGACATTGTTTCTT+GGG 0.286701 2.2:-63863686 MS.gene001530:CDS
TCTTCTGGTTCAACAAAATC+AGG 0.301153 2.2:+63862053 None:intergenic
TAAGGTAATTTAGACTCTTT+TGG 0.303752 2.2:+63861911 None:intergenic
TGAAACTTGAGAAGTTATTA+TGG 0.310950 2.2:+63863590 None:intergenic
TCTCACCAAGACTTATGATA+TGG 0.311693 2.2:-63863722 MS.gene001530:CDS
ATGCTAGCTACATTACAAAA+AGG 0.320103 2.2:+63862521 None:intergenic
GGCTCAGTTGCAACCGGATT+AGG 0.358087 2.2:+63862131 None:intergenic
CGAGTCGAGTTGGCCAATAT+TGG 0.365906 2.2:-63861938 MS.gene001530:CDS
GGCTCAGTTGCATCATGATT+AGG 0.376887 2.2:+63862170 None:intergenic
TTATTATAATCAGTAGTAAC+CGG 0.377060 2.2:+63862011 None:intergenic
CGGATGTCTTTGAAAATAAG+AGG 0.384067 2.2:+63862358 None:intergenic
AAGAAACCTGGGAATGAGTT+AGG 0.397323 2.2:-63862599 MS.gene001530:CDS
ACAGTACTCGAAGGGTCATT+TGG 0.400137 2.2:-63862675 MS.gene001530:CDS
GACTCAGTTGCAACTGGATC+AGG 0.430111 2.2:+63862209 None:intergenic
TGTAATCGTTGTTCCTAATC+CGG 0.434226 2.2:-63862144 MS.gene001530:CDS
ACCGCTGGCTCAGTTGCAAC+CGG 0.439728 2.2:+63862125 None:intergenic
GTTTCTTGAACTTCAGAGAA+AGG 0.439975 2.2:-63862729 MS.gene001530:CDS
GGGAATGATGGAAGAAGCTC+AGG 0.447000 2.2:-63863773 None:intergenic
CATGTCGACTTCTAGTGACT+TGG 0.447575 2.2:+63862256 None:intergenic
GAGAAAAGGAGGCACCTTAT+CGG 0.464277 2.2:+63863741 None:intergenic
TTGAATCATCAATGACAAGT+TGG 0.472901 2.2:-63862406 MS.gene001530:CDS
ACATCCGACTCAGTTGCAAC+TGG 0.474094 2.2:+63862203 None:intergenic
TCATAAGTCTTGGTGAGAAA+AGG 0.480198 2.2:+63863727 None:intergenic
GATGACTCAATGAATTATGT+AGG 0.484303 2.2:-63862323 MS.gene001530:CDS
ACAATACTTGCAGGACGAAT+CGG 0.489021 2.2:-63861976 MS.gene001530:CDS
CTCAACCATATCATAAGTCT+TGG 0.489284 2.2:+63863717 None:intergenic
CATGAGCATTCTGCGAAGAA+TGG 0.490641 2.2:+63862840 None:intergenic
CATATACCTAACTCATTCCC+AGG 0.495219 2.2:+63862593 None:intergenic
GAGTCATCAAAATCTACATT+CGG 0.497020 2.2:+63862338 None:intergenic
TGAACTTCAGAGAAAGGAAG+AGG 0.498686 2.2:-63862723 MS.gene001530:CDS
TCGCAGAATGCTCATGGCCA+AGG 0.501991 2.2:-63862833 MS.gene001530:CDS
GGTCAAGTGCTGAAGAAACC+TGG 0.503718 2.2:-63862611 MS.gene001530:CDS
TTATTATGGTTAAAGTATTG+AGG 0.507346 2.2:+63863604 None:intergenic
CGCAGAATGCTCATGGCCAA+GGG 0.508087 2.2:-63862832 MS.gene001530:CDS
TGAATCATCAATGACAAGTT+GGG 0.509603 2.2:-63862405 MS.gene001530:CDS
GAAACAATGTCATCAGTTGA+AGG 0.519956 2.2:+63863691 None:intergenic
TTAGGAACAACGATTACATT+CGG 0.521576 2.2:+63862149 None:intergenic
CAATATTGGCCAACTCGACT+CGG 0.523538 2.2:+63861939 None:intergenic
CTAGTGGAGCAGCAACCCCT+TGG 0.524076 2.2:+63862816 None:intergenic
TCAGGGTAGTAGTTCCGATA+AGG 0.544906 2.2:-63863755 MS.gene001530:CDS
CATTCTTCGCAGAATGCTCA+TGG 0.549403 2.2:-63862839 MS.gene001530:CDS
TGTCGATCGGATTCTGCTAG+TGG 0.551922 2.2:+63862800 None:intergenic
CAAAGTGTGCTCTCTTATGT+TGG 0.554182 2.2:-63862632 MS.gene001530:CDS
ATGTCGACTTCTAGTGACTT+GGG 0.560272 2.2:+63862257 None:intergenic
CAAGACTTATGATATGGTTG+AGG 0.564774 2.2:-63863716 MS.gene001530:CDS
AGCATTCAATGTCTGTCGAT+CGG 0.568118 2.2:+63862787 None:intergenic
AGCTAGCATTGAAGATCATG+TGG 0.568546 2.2:-63862507 MS.gene001530:CDS
GGATCAGGAACAACTACCAC+TGG 0.574913 2.2:+63862224 None:intergenic
TCGGAAGCATCGATTACCGC+TGG 0.577204 2.2:+63862110 None:intergenic
GATAAAATTACAGTACTCGA+AGG 0.578742 2.2:-63862684 MS.gene001530:CDS
ACTGATGACATTGTTTCTTG+GGG 0.579964 2.2:-63863685 MS.gene001530:CDS
GTCAAGTGCTGAAGAAACCT+GGG 0.583533 2.2:-63862610 MS.gene001530:CDS
TCAGGGACAGCTGCAACAGT+TGG 0.583782 2.2:+63862071 None:intergenic
GAAGATATACCGAGTCGAGT+TGG 0.590581 2.2:-63861948 MS.gene001530:CDS
TATTATAATCAGTAGTAACC+GGG 0.593232 2.2:+63862012 None:intergenic
AGGAACAACTACCACTGGCA+TGG 0.597086 2.2:+63862229 None:intergenic
CTTCTGGTTCAACAAAATCA+GGG 0.603287 2.2:+63862054 None:intergenic
TGATCCAGTTGCAACTGAGT+CGG 0.607702 2.2:-63862207 MS.gene001530:CDS
TAAGTCTTGGTGAGAAAAGG+AGG 0.608589 2.2:+63863730 None:intergenic
TGGCTCAACTACAACAAGAT+CGG 0.609117 2.2:+63862091 None:intergenic
TCCGGTTGCAACTGAGCCAG+CGG 0.609490 2.2:-63862126 MS.gene001530:CDS
GTCATTTGGAAACAGTGGAA+AGG 0.611941 2.2:-63862661 MS.gene001530:CDS
ATCTTATGAAGAATATCCAA+AGG 0.635890 2.2:-63862880 MS.gene001530:CDS
ATAAAATTACAGTACTCGAA+GGG 0.640506 2.2:-63862683 MS.gene001530:CDS
GTAACCGGGGATGTTACGAC+GGG 0.648034 2.2:+63862026 None:intergenic
ACAACCCGTCGTAACATCCC+CGG 0.648155 2.2:-63862030 MS.gene001530:CDS
ATCATCAATGACAAGTTGGG+AGG 0.657482 2.2:-63862402 MS.gene001530:CDS
GTCTCTTCTATCATTAAACG+TGG 0.666811 2.2:-63862444 MS.gene001530:CDS
CGACATGAACTCCATGCCAG+TGG 0.675176 2.2:-63862240 MS.gene001530:CDS
GCTAGCATTGAAGATCATGT+GGG 0.675912 2.2:-63862506 MS.gene001530:CDS
AATGATAGAAGAGACACACT+CGG 0.677326 2.2:+63862452 None:intergenic
ATGATAGAAGAGACACACTC+GGG 0.678625 2.2:+63862453 None:intergenic
AGTAACCGGGGATGTTACGA+CGG 0.681684 2.2:+63862025 None:intergenic
GAAGGGTCATTTGGAAACAG+TGG 0.685082 2.2:-63862666 MS.gene001530:CDS
ATTATAATCAGTAGTAACCG+GGG 0.692233 2.2:+63862013 None:intergenic
GCAGAATGCTCATGGCCAAG+GGG 0.714345 2.2:-63862831 MS.gene001530:CDS
GGAATGATGGAAGAAGCTCA+GGG 0.733848 2.2:-63863772 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATTATAATAATGAATTTT+GGG - chr2.2:63863686-63863705 MS.gene001530:CDS 10.0%
!!! TTATTGTTTTTTTATTAAGT+TGG + chr2.2:63862557-63862576 None:intergenic 10.0%
!! TTAAATAAATCAATCAAATC+AGG + chr2.2:63862355-63862374 None:intergenic 15.0%
!!! CTGATTATAATAATGAATTT+TGG - chr2.2:63863685-63863704 MS.gene001530:CDS 15.0%
!!! GATTATAATAATGAATTTTG+GGG - chr2.2:63863687-63863706 MS.gene001530:CDS 15.0%
!! AAAAATTGATACACAACTAT+GGG - chr2.2:63862747-63862766 MS.gene001530:CDS 20.0%
!! TTATTATAATCAGTAGTAAC+CGG + chr2.2:63863678-63863697 None:intergenic 20.0%
!!! TTAAGCTTATGTATTTTCAT+AGG - chr2.2:63862371-63862390 MS.gene001530:CDS 20.0%
!!! TTATTATGGTTAAAGTATTG+AGG + chr2.2:63862085-63862104 None:intergenic 20.0%
! AAGCAATAAAAAAACCTGAA+TGG + chr2.2:63862255-63862274 None:intergenic 25.0%
! AAGCAATCAATAAACCTTAA+TGG + chr2.2:63862689-63862708 None:intergenic 25.0%
! AGCAATCAATAAACCTTAAT+GGG + chr2.2:63862688-63862707 None:intergenic 25.0%
! ATAAAAGAACAAAGCTTGAT+TGG + chr2.2:63862512-63862531 None:intergenic 25.0%
! ATAAAATTACAGTACTCGAA+GGG - chr2.2:63863003-63863022 MS.gene001530:intron 25.0%
! ATCTTATGAAGAATATCCAA+AGG - chr2.2:63862806-63862825 MS.gene001530:CDS 25.0%
! CAAAAACTACAAAGCAAAAA+AGG - chr2.2:63863124-63863143 MS.gene001530:intron 25.0%
! CTTTAATGTACTTCATTTCA+TGG - chr2.2:63862147-63862166 MS.gene001530:CDS 25.0%
! GAAAAATTGATACACAACTA+TGG - chr2.2:63862746-63862765 MS.gene001530:CDS 25.0%
! GAGGAGATTAAAAATTATCT+TGG - chr2.2:63863303-63863322 MS.gene001530:intron 25.0%
! TATTATAATCAGTAGTAACC+GGG + chr2.2:63863677-63863696 None:intergenic 25.0%
! TGAAACTTGAGAAGTTATTA+TGG + chr2.2:63862099-63862118 None:intergenic 25.0%
!! TTACAAAAAGGATTGTTTCT+TGG + chr2.2:63863156-63863175 None:intergenic 25.0%
!! TTAGACTGTGTTATGTTTTT+CGG - chr2.2:63862273-63862292 MS.gene001530:CDS 25.0%
!! TTTTTCTAATCATTCCATTC+AGG - chr2.2:63862238-63862257 MS.gene001530:CDS 25.0%
!!! CTGATGAAAGTTTTATTGTT+TGG - chr2.2:63862029-63862048 MS.gene001530:CDS 25.0%
AAACTACAAAGCAAAAAAGG+AGG - chr2.2:63863127-63863146 MS.gene001530:intron 30.0%
AGTTGCATACAAGATGTTAT+TGG + chr2.2:63863393-63863412 None:intergenic 30.0%
ATGCTAGCTACATTACAAAA+AGG + chr2.2:63863168-63863187 None:intergenic 30.0%
ATTATAATCAGTAGTAACCG+GGG + chr2.2:63863676-63863695 None:intergenic 30.0%
GAGTCATCAAAATCTACATT+CGG + chr2.2:63863351-63863370 None:intergenic 30.0%
GATAAAATTACAGTACTCGA+AGG - chr2.2:63863002-63863021 MS.gene001530:intron 30.0%
GATGACTCAATGAATTATGT+AGG - chr2.2:63863363-63863382 MS.gene001530:intron 30.0%
GTTAGAAAAATTGATCATCG+TGG - chr2.2:63862308-63862327 MS.gene001530:CDS 30.0%
TGAATCATCAATGACAAGTT+GGG - chr2.2:63863281-63863300 MS.gene001530:intron 30.0%
TGTGAGATAGCTTATGTTAT+AGG - chr2.2:63862466-63862485 MS.gene001530:CDS 30.0%
TTAGGAACAACGATTACATT+CGG + chr2.2:63863540-63863559 None:intergenic 30.0%
TTGAATCATCAATGACAAGT+TGG - chr2.2:63863280-63863299 MS.gene001530:intron 30.0%
! AACTGATGACATTGTTTCTT+GGG - chr2.2:63862000-63862019 MS.gene001530:CDS 30.0%
! CTTCTTTTTATCACCCATTA+AGG - chr2.2:63862672-63862691 MS.gene001530:CDS 30.0%
! TTGAACTGTCTTATGGTTTT+TGG - chr2.2:63862707-63862726 MS.gene001530:CDS 30.0%
!!! TATGGTTTTTGGTTTGATCA+GGG - chr2.2:63862718-63862737 MS.gene001530:CDS 30.0%
!!! TTATGGTTTTTGGTTTGATC+AGG - chr2.2:63862717-63862736 MS.gene001530:CDS 30.0%
AATGATAGAAGAGACACACT+CGG + chr2.2:63863237-63863256 None:intergenic 35.0%
CAAGACTTATGATATGGTTG+AGG - chr2.2:63861970-63861989 MS.gene001530:CDS 35.0%
CAGCTTAATACCTATGTAAG+CGG - chr2.2:63862124-63862143 MS.gene001530:CDS 35.0%
CTCAACCATATCATAAGTCT+TGG + chr2.2:63861972-63861991 None:intergenic 35.0%
GAAACAATGTCATCAGTTGA+AGG + chr2.2:63861998-63862017 None:intergenic 35.0%
GTCTCTTCTATCATTAAACG+TGG - chr2.2:63863242-63863261 MS.gene001530:intron 35.0%
GTTTCTTGAACTTCAGAGAA+AGG - chr2.2:63862957-63862976 MS.gene001530:intron 35.0%
TACATTAAAGCCGCTTACAT+AGG + chr2.2:63862137-63862156 None:intergenic 35.0%
TCATAAGTCTTGGTGAGAAA+AGG + chr2.2:63861962-63861981 None:intergenic 35.0%
TCTCACCAAGACTTATGATA+TGG - chr2.2:63861964-63861983 MS.gene001530:CDS 35.0%
TGATTGCTTGAACTGTCTTA+TGG - chr2.2:63862700-63862719 MS.gene001530:CDS 35.0%
TGTAATCGTTGTTCCTAATC+CGG - chr2.2:63863542-63863561 MS.gene001530:intron 35.0%
! ACTGATGACATTGTTTCTTG+GGG - chr2.2:63862001-63862020 MS.gene001530:CDS 35.0%
! CAACTGATGACATTGTTTCT+TGG - chr2.2:63861999-63862018 MS.gene001530:CDS 35.0%
! CGGATGTCTTTGAAAATAAG+AGG + chr2.2:63863331-63863350 None:intergenic 35.0%
! TATAAACTGTTTTGCGCCTT+TGG + chr2.2:63862825-63862844 None:intergenic 35.0%
! TGCTTTGTAGTTTTTGTCAG+CGG + chr2.2:63863120-63863139 None:intergenic 35.0%
!! CAGAAGATCAAAGCATTGTT+TGG + chr2.2:63862639-63862658 None:intergenic 35.0%
!! CTTCTGGTTCAACAAAATCA+GGG + chr2.2:63863635-63863654 None:intergenic 35.0%
!! TCTTCTGGTTCAACAAAATC+AGG + chr2.2:63863636-63863655 None:intergenic 35.0%
AAGAAACCTGGGAATGAGTT+AGG - chr2.2:63863087-63863106 MS.gene001530:intron 40.0%
ACAATACTTGCAGGACGAAT+CGG - chr2.2:63863710-63863729 MS.gene001530:CDS 40.0%
AGCATTCAATGTCTGTCGAT+CGG + chr2.2:63862902-63862921 None:intergenic 40.0%
ATGATAGAAGAGACACACTC+GGG + chr2.2:63863236-63863255 None:intergenic 40.0%
CAAAGTGTGCTCTCTTATGT+TGG - chr2.2:63863054-63863073 MS.gene001530:intron 40.0%
CATATACCTAACTCATTCCC+AGG + chr2.2:63863096-63863115 None:intergenic 40.0%
GTCATTTGGAAACAGTGGAA+AGG - chr2.2:63863025-63863044 MS.gene001530:intron 40.0%
TAAGTCTTGGTGAGAAAAGG+AGG + chr2.2:63861959-63861978 None:intergenic 40.0%
TGAACTTCAGAGAAAGGAAG+AGG - chr2.2:63862963-63862982 MS.gene001530:intron 40.0%
TGGCTCAACTACAACAAGAT+CGG + chr2.2:63863598-63863617 None:intergenic 40.0%
! AGCTAGCATTGAAGATCATG+TGG - chr2.2:63863179-63863198 MS.gene001530:intron 40.0%
! ATCATCAATGACAAGTTGGG+AGG - chr2.2:63863284-63863303 MS.gene001530:intron 40.0%
! CTCTTTTGGTACGCCAATAT+TGG + chr2.2:63863764-63863783 None:intergenic 40.0%
! GCTAGCATTGAAGATCATGT+GGG - chr2.2:63863180-63863199 MS.gene001530:intron 40.0%
!! ATGTCGACTTCTAGTGACTT+GGG + chr2.2:63863432-63863451 None:intergenic 40.0%
!! ATTTTGGGGACAATACTTGC+AGG - chr2.2:63863701-63863720 MS.gene001530:CDS 40.0%
ACAGTACTCGAAGGGTCATT+TGG - chr2.2:63863011-63863030 MS.gene001530:intron 45.0%
CAATATTGGCCAACTCGACT+CGG + chr2.2:63863750-63863769 None:intergenic 45.0%
CATGAGCATTCTGCGAAGAA+TGG + chr2.2:63862849-63862868 None:intergenic 45.0%
CATTCTTCGCAGAATGCTCA+TGG - chr2.2:63862847-63862866 MS.gene001530:CDS 45.0%
GAAGATATACCGAGTCGAGT+TGG - chr2.2:63863738-63863757 MS.gene001530:CDS 45.0%
GAAGGGTCATTTGGAAACAG+TGG - chr2.2:63863020-63863039 MS.gene001530:intron 45.0%
GGCTCAGTTGCATCATGATT+AGG + chr2.2:63863519-63863538 None:intergenic 45.0%
TCAGGGTAGTAGTTCCGATA+AGG - chr2.2:63861931-63861950 MS.gene001530:CDS 45.0%
TGATCCAGTTGCAACTGAGT+CGG - chr2.2:63863479-63863498 MS.gene001530:intron 45.0%
!! CATGTCGACTTCTAGTGACT+TGG + chr2.2:63863433-63863452 None:intergenic 45.0%
!! GAGAAAAGGAGGCACCTTAT+CGG + chr2.2:63861948-63861967 None:intergenic 45.0%
!! GTCAAGTGCTGAAGAAACCT+GGG - chr2.2:63863076-63863095 MS.gene001530:intron 45.0%
ACATCCGACTCAGTTGCAAC+TGG + chr2.2:63863486-63863505 None:intergenic 50.0%
AGGAACAACTACCACTGGCA+TGG + chr2.2:63863460-63863479 None:intergenic 50.0%
AGTAACCGGGGATGTTACGA+CGG + chr2.2:63863664-63863683 None:intergenic 50.0%
GACTCAGTTGCAACTGGATC+AGG + chr2.2:63863480-63863499 None:intergenic 50.0%
GGATCAGGAACAACTACCAC+TGG + chr2.2:63863465-63863484 None:intergenic 50.0%
TGTCGATCGGATTCTGCTAG+TGG + chr2.2:63862889-63862908 None:intergenic 50.0%
!! CGAGTCGAGTTGGCCAATAT+TGG - chr2.2:63863748-63863767 MS.gene001530:CDS 50.0%
!! GGTCAAGTGCTGAAGAAACC+TGG - chr2.2:63863075-63863094 MS.gene001530:intron 50.0%
!! GTTACGACGGGTTGTTCTTC+TGG + chr2.2:63863651-63863670 None:intergenic 50.0%
ACAACCCGTCGTAACATCCC+CGG - chr2.2:63863656-63863675 MS.gene001530:CDS 55.0%
CGACATGAACTCCATGCCAG+TGG - chr2.2:63863446-63863465 MS.gene001530:intron 55.0%
CGCAGAATGCTCATGGCCAA+GGG - chr2.2:63862854-63862873 MS.gene001530:CDS 55.0%
GCAGAATGCTCATGGCCAAG+GGG - chr2.2:63862855-63862874 MS.gene001530:CDS 55.0%
GGCTCAGTTGCAACCGGATT+AGG + chr2.2:63863558-63863577 None:intergenic 55.0%
GTAACCGGGGATGTTACGAC+GGG + chr2.2:63863663-63863682 None:intergenic 55.0%
TCAGGGACAGCTGCAACAGT+TGG + chr2.2:63863618-63863637 None:intergenic 55.0%
TCGCAGAATGCTCATGGCCA+AGG - chr2.2:63862853-63862872 MS.gene001530:CDS 55.0%
!! TCGGAAGCATCGATTACCGC+TGG + chr2.2:63863579-63863598 None:intergenic 55.0%
CTAGTGGAGCAGCAACCCCT+TGG + chr2.2:63862873-63862892 None:intergenic 60.0%
TCCGGTTGCAACTGAGCCAG+CGG - chr2.2:63863560-63863579 MS.gene001530:intron 60.0%
!! ACCGCTGGCTCAGTTGCAAC+CGG + chr2.2:63863564-63863583 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 63861920 63863788 63861920 ID=MS.gene001530
chr2.2 mRNA 63861920 63863788 63861920 ID=MS.gene001530.t1;Parent=MS.gene001530
chr2.2 exon 63863570 63863788 63863570 ID=MS.gene001530.t1.exon1;Parent=MS.gene001530.t1
chr2.2 CDS 63863570 63863788 63863570 ID=cds.MS.gene001530.t1;Parent=MS.gene001530.t1
chr2.2 exon 63861920 63862969 63861920 ID=MS.gene001530.t1.exon2;Parent=MS.gene001530.t1
chr2.2 CDS 63861920 63862969 63861920 ID=cds.MS.gene001530.t1;Parent=MS.gene001530.t1
Gene Sequence

>MS.gene001530

ATGGAAGAAGCTCAGGGTAGTAGTTCCGATAAGGTGCCTCCTTTTCTCACCAAGACTTATGATATGGTTGAGGATCCTTCAACTGATGACATTGTTTCTTGGGGTGCTACTGATGAAAGTTTTATTGTTTGGAATAAACTAGATTTTGAAAAAGATGTGTTGCCTCAATACTTTAACCATAATAACTTCTCAAGTTTCATCAGACAGCTTAATACCTATGTAAGCGGCTTTAATGTACTTCATTTCATGGATTTTTAAATTGTTTTGTTATTTCGTTAATGTATAATGTTTTGTTATTGTCTTAATGTTGTCTCTTTTTTTTTCTAATCATTCCATTCAGGTTTTTTTATTGCTTAGACTGTGTTATGTTTTTCGGTTTGATTATGTCGTTAGAAAAATTGATCATCGTGGATTTGTTAATTTTTTTATGAGCCTGATTTGATTGATTTATTTAAGCTTATGTATTTTCATAGGCACGCGTAAGACTAATTGAGATAGCTTATAGAAAAACAACTTCATAAGTTGTTCAGTTTATTTTATTTTCATTGTGAGATAGCTTATGTTATAGGCACTTTATTAAGTTGTTGATCCAATCAAGCTTTGTTCTTTTATTGTTACTGTATTTTCATCACTGCCAACTTAATAAAAAAACAATAACAATAACAACAAAAATCATATAAGTGTTTATGTTATAAACACTCAATTAACTTGCTCATCCAAACAATGCTTTGATCTTCTGTTTTTTTCTCTCTCTTCTTTTTATCACCCATTAAGGTTTATTGATTGCTTGAACTGTCTTATGGTTTTTGGTTTGATCAGGGCTTTAGAAAAATTGATACACAACTATGGGAATACGCAAACGATGATTTCGTAAGAAATCAAAAACATCTTATGAAGAATATCCAAAGGCGCAAAACAGTTTATAGCCATTCTTCGCAGAATGCTCATGGCCAAGGGGTTGCTGCTCCACTAGCAGAATCCGATCGACAGACATTGAATGCTCATATTCAAAAACTTAAACACGACAATGAACAAATGTTTCTTGAACTTCAGAGAAAGGAAGAGGAGAGAAAAATGCATGAGATAAAATTACAGTACTCGAAGGGTCATTTGGAAACAGTGGAAAGGAAACATCAAAGTGTGCTCTCTTATGTTGGTCAAGTGCTGAAGAAACCTGGGAATGAGTTAGGTATATGTCCGCTGACAAAAACTACAAAGCAAAAAAGGAGGATTCCAAGAAACAATCCTTTTTGTAATGTAGCTAGCATTGAAGATCATGTGGGAACTTCTCATGTAATACATAAAGAAAACGACCCGAGTGTGTCTCTTCTATCATTAAACGTGGAACAATTAAATTTGCTTGAATCATCAATGACAAGTTGGGAGGAGATTAAAAATTATCTTGGTGCCTCTTATTTTCAAAGACATCCGAATGTAGATTTTGATGACTCAATGAATTATGTAGGCTGTCCAATAACATCTTGTATGCAACTAGATGTTGAAGTTCAGCCCAAGTCACTAGAAGTCGACATGAACTCCATGCCAGTGGTAGTTGTTCCTGATCCAGTTGCAACTGAGTCGGATGTAGTTGTTAATCCTAATCATGATGCAACTGAGCCGAATGTAATCGTTGTTCCTAATCCGGTTGCAACTGAGCCAGCGGTAATCGATGCTTCCGATCTTGTTGTAGTTGAGCCAACTGTTGCAGCTGTCCCTGATTTTGTTGAACCAGAAGAACAACCCGTCGTAACATCCCCGGTTACTACTGATTATAATAATGAATTTTGGGGACAATACTTGCAGGACGAATCGGAAAATGAAGATATACCGAGTCGAGTTGGCCAATATTGGCGTACCAAAAGAGTCTAA
Protein sequence

>MS.gene001530.t1

MEEAQGSSSDKVPPFLTKTYDMVEDPSTDDIVSWGATDESFIVWNKLDFEKDVLPQYFNHNNFSSFIRQLNTYGFRKIDTQLWEYANDDFVRNQKHLMKNIQRRKTVYSHSSQNAHGQGVAAPLAESDRQTLNAHIQKLKHDNEQMFLELQRKEEERKMHEIKLQYSKGHLETVERKHQSVLSYVGQVLKKPGNELGICPLTKTTKQKRRIPRNNPFCNVASIEDHVGTSHVIHKENDPSVSLLSLNVEQLNLLESSMTSWEEIKNYLGASYFQRHPNVDFDDSMNYVGCPITSCMQLDVEVQPKSLEVDMNSMPVVVVPDPVATESDVVVNPNHDATEPNVIVVPNPVATEPAVIDASDLVVVEPTVAAVPDFVEPEEQPVVTSPVTTDYNNEFWGQYLQDESENEDIPSRVGQYWRTKRV