Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001558.t1 | RHN42394.1 | 91.5 | 106 | 9 | 0 | 4 | 109 | 26 | 131 | 4.50E-43 | 183.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001558.t1 | Q8LBM2 | 44.2 | 95 | 48 | 3 | 11 | 102 | 36 | 128 | 1.3e-09 | 63.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001558.t1 | A0A396GSB0 | 91.5 | 106 | 9 | 0 | 4 | 109 | 26 | 131 | 3.2e-43 | 183.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene001558.t1 | TF | Tify |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001558.t1 | MTR_2g019190 | 60.000 | 95 | 27 | 3 | 17 | 102 | 41 | 133 | 1.62e-31 | 108 |
| MS.gene001558.t1 | MTR_8g107300 | 57.292 | 96 | 25 | 4 | 10 | 101 | 33 | 116 | 8.55e-28 | 98.6 |
| MS.gene001558.t1 | MTR_8g107300 | 59.770 | 87 | 22 | 3 | 19 | 101 | 4 | 81 | 4.22e-27 | 95.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001558.t1 | AT1G30135 | 46.988 | 83 | 41 | 2 | 23 | 102 | 46 | 128 | 1.57e-15 | 67.4 |
Find 19 sgRNAs with CRISPR-Local
Find 113 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATCCGATTCTTCCTCTTT+TGG | 0.250942 | 4.1:+16118956 | None:intergenic |
| GACTGATGCCACTGAGATTC+AGG | 0.303605 | 4.1:-16121019 | MS.gene001558:intron |
| TCTTCTCATTTATCTTAAGA+TGG | 0.423225 | 4.1:+16118917 | None:intergenic |
| TTCTCCAAAAGAGGAAGAAT+CGG | 0.429979 | 4.1:-16118960 | MS.gene001558:CDS |
| TCATTTATCTTAAGATGGTA+AGG | 0.430837 | 4.1:+16118922 | None:intergenic |
| GAAATGGTGAGGACACCAAC+TGG | 0.450322 | 4.1:-16119069 | MS.gene001558:CDS |
| AAGATCATAAGCAGCAGTAC+AGG | 0.470194 | 4.1:-16121077 | MS.gene001558:CDS |
| TGCAGAGGTTTCTCCAAAAG+AGG | 0.484466 | 4.1:-16118969 | MS.gene001558:CDS |
| TGCTGCTTATGATCTTCTTG+TGG | 0.500195 | 4.1:+16121084 | None:intergenic |
| CCGTTGACCATTGTCTATGA+TGG | 0.521959 | 4.1:-16121054 | MS.gene001558:CDS |
| CATTTATCTTAAGATGGTAA+GGG | 0.531668 | 4.1:+16118923 | None:intergenic |
| TGCATGGAAAGAGAAGTACT+AGG | 0.548727 | 4.1:+16119000 | None:intergenic |
| TGCAAGCATTAAAATGGACC+TGG | 0.550717 | 4.1:+16119098 | None:intergenic |
| GCATGGAAAGAGAAGTACTA+GGG | 0.573020 | 4.1:+16119001 | None:intergenic |
| ATTTATCTTAAGATGGTAAG+GGG | 0.589050 | 4.1:+16118924 | None:intergenic |
| AATCTTGCCATCATAGACAA+TGG | 0.643141 | 4.1:+16121047 | None:intergenic |
| CCATCATAGACAATGGTCAA+CGG | 0.652322 | 4.1:+16121054 | None:intergenic |
| GGCTCTGATGATGATCCAGT+TGG | 0.655026 | 4.1:+16119054 | None:intergenic |
| TGAACACACCTGAATCTCAG+TGG | 0.730872 | 4.1:+16121011 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTAATTTTAATATATTA+AGG | + | chr4.1:16120735-16120754 | None:intergenic | 0.0% |
| !!! | TTTTTTTATTTTTTAAAATA+TGG | - | chr4.1:16119634-16119653 | MS.gene001558:intron | 0.0% |
| !! | ATAATAGTTTGAATTAAAAT+TGG | - | chr4.1:16120863-16120882 | MS.gene001558:intron | 10.0% |
| !!! | TAAGTAAAAAAACAATTTTT+AGG | + | chr4.1:16119458-16119477 | None:intergenic | 10.0% |
| !! | CATTGAATAAATAATGTATA+TGG | - | chr4.1:16119394-16119413 | MS.gene001558:intron | 15.0% |
| !! | GAAAAAAAAATCAAAAAACA+TGG | + | chr4.1:16120679-16120698 | None:intergenic | 15.0% |
| !!! | ATATACGTTTTTTATTTTTC+TGG | + | chr4.1:16119505-16119524 | None:intergenic | 15.0% |
| !!! | CTATTTATTAATTTTTTCTG+CGG | - | chr4.1:16120227-16120246 | MS.gene001558:intron | 15.0% |
| !!! | TATGCAATTTTTGTTTTTTT+AGG | + | chr4.1:16119211-16119230 | None:intergenic | 15.0% |
| !!! | TTTTTACTTATAAATGTGAA+CGG | - | chr4.1:16119468-16119487 | MS.gene001558:intron | 15.0% |
| !! | AGCTACAAATTTAGTAATAA+AGG | - | chr4.1:16120444-16120463 | MS.gene001558:intron | 20.0% |
| !! | ATTAAAAGAGAGAAGATAAA+TGG | + | chr4.1:16119791-16119810 | None:intergenic | 20.0% |
| !! | CAATGATATTAGTACTTAAT+AGG | + | chr4.1:16120276-16120295 | None:intergenic | 20.0% |
| !! | CATAGAATAATTAATGTATC+TGG | + | chr4.1:16119433-16119452 | None:intergenic | 20.0% |
| !! | GATATAAATAAATCAGTAAG+CGG | - | chr4.1:16120531-16120550 | MS.gene001558:intron | 20.0% |
| !! | GCTACAAATTTAGTAATAAA+GGG | - | chr4.1:16120445-16120464 | MS.gene001558:intron | 20.0% |
| !! | TAAAATTGGAATTAAGTAGT+GGG | - | chr4.1:16120877-16120896 | MS.gene001558:intron | 20.0% |
| !! | TATAAATGCAAACTACAAAT+TGG | - | chr4.1:16120477-16120496 | MS.gene001558:intron | 20.0% |
| !! | TTAAAATTGGAATTAAGTAG+TGG | - | chr4.1:16120876-16120895 | MS.gene001558:intron | 20.0% |
| !!! | AATTTGCTTATAAATGTGAA+CGG | + | chr4.1:16119148-16119167 | None:intergenic | 20.0% |
| !!! | ATTTGTAGTTTGCATTTATA+AGG | + | chr4.1:16120477-16120496 | None:intergenic | 20.0% |
| !!! | TAAGCAAAACACAATTTTTA+AGG | - | chr4.1:16119369-16119388 | MS.gene001558:intron | 20.0% |
| !!! | TCAAACTATTATTGATGATA+AGG | + | chr4.1:16120855-16120874 | None:intergenic | 20.0% |
| !!! | TCTTTTCATCATATAATCTT+TGG | + | chr4.1:16120411-16120430 | None:intergenic | 20.0% |
| !!! | TTTTTTTGCAAGCATTAAAA+TGG | + | chr4.1:16120940-16120959 | None:intergenic | 20.0% |
| ! | AAAATTGGAATTAAGTAGTG+GGG | - | chr4.1:16120878-16120897 | MS.gene001558:intron | 25.0% |
| ! | AAATTGCATATAAATGTGAC+CGG | - | chr4.1:16119221-16119240 | MS.gene001558:intron | 25.0% |
| ! | AATGCTTGCAAAAAAAGAAA+TGG | - | chr4.1:16120944-16120963 | MS.gene001558:intron | 25.0% |
| ! | ATGCATTATTGTACATGATA+TGG | + | chr4.1:16119329-16119348 | None:intergenic | 25.0% |
| ! | ATGTGAATTGTATATATTGC+AGG | - | chr4.1:16120908-16120927 | MS.gene001558:intron | 25.0% |
| ! | ATTTATCTTAAGATGGTAAG+GGG | + | chr4.1:16121108-16121127 | None:intergenic | 25.0% |
| ! | CATTTATCTTAAGATGGTAA+GGG | + | chr4.1:16121109-16121128 | None:intergenic | 25.0% |
| ! | CTATACAAGTTAAAAACTGA+TGG | + | chr4.1:16121008-16121027 | None:intergenic | 25.0% |
| ! | TCATTTATCTTAAGATGGTA+AGG | + | chr4.1:16121110-16121129 | None:intergenic | 25.0% |
| ! | TCTTCTCATTTATCTTAAGA+TGG | + | chr4.1:16121115-16121134 | None:intergenic | 25.0% |
| ! | TGTGTACAATTTATTACGTA+TGG | + | chr4.1:16120158-16120177 | None:intergenic | 25.0% |
| ! | TTTATATCTTTCGTTTGTCT+TGG | + | chr4.1:16120519-16120538 | None:intergenic | 25.0% |
| !! | ACAACATAACACTTCATTTT+TGG | + | chr4.1:16120346-16120365 | None:intergenic | 25.0% |
| !! | ATTTGTTGACCTCAAAATTT+GGG | - | chr4.1:16120618-16120637 | MS.gene001558:intron | 25.0% |
| !! | TATTAATTCTGGTGCTAAAT+AGG | + | chr4.1:16120048-16120067 | None:intergenic | 25.0% |
| !! | TTTTCAAAAAATGCATATGC+AGG | - | chr4.1:16119549-16119568 | MS.gene001558:intron | 25.0% |
| AATAGCATCATCATCTAGTT+AGG | - | chr4.1:16120755-16120774 | MS.gene001558:intron | 30.0% | |
| ACAAGAATTAAAGAGAGAGA+GGG | + | chr4.1:16120132-16120151 | None:intergenic | 30.0% | |
| AGAGATTCAGAAATTACTCA+AGG | - | chr4.1:16119858-16119877 | MS.gene001558:intron | 30.0% | |
| TCTCTCTCTTTAATTCTTGT+GGG | - | chr4.1:16120132-16120151 | MS.gene001558:intron | 30.0% | |
| TTGAATGAAAGTTTGGTTGA+GGG | + | chr4.1:16119754-16119773 | None:intergenic | 30.0% | |
| TTGCAAAAAAAGAAATGGTG+AGG | - | chr4.1:16120949-16120968 | MS.gene001558:intron | 30.0% | |
| ! | AAGCATTTTCTTTCCACAAT+GGG | + | chr4.1:16120077-16120096 | None:intergenic | 30.0% |
| ! | AATAGTTAGAGCATTCACAA+TGG | - | chr4.1:16120011-16120030 | MS.gene001558:intron | 30.0% |
| ! | ATGAAGTGTTATGTTGTAGA+TGG | - | chr4.1:16120349-16120368 | MS.gene001558:intron | 30.0% |
| ! | CATTTGTTGACCTCAAAATT+TGG | - | chr4.1:16120617-16120636 | MS.gene001558:intron | 30.0% |
| ! | CGGATTACTCAAAAAACTTT+TGG | + | chr4.1:16119683-16119702 | None:intergenic | 30.0% |
| ! | GTTTTGCTTATAAATGTGAC+CGG | + | chr4.1:16119359-16119378 | None:intergenic | 30.0% |
| ! | TAAGGTGAGTTGAAAAAGAA+TGG | + | chr4.1:16120717-16120736 | None:intergenic | 30.0% |
| ! | TTAAAAACTGATGGTGAAGA+TGG | + | chr4.1:16120999-16121018 | None:intergenic | 30.0% |
| !!! | CAAATTTTGAGGTCAACAAA+TGG | + | chr4.1:16120619-16120638 | None:intergenic | 30.0% |
| !!! | GTTTTTTGAGTAATCCGTTT+TGG | - | chr4.1:16119686-16119705 | MS.gene001558:intron | 30.0% |
| !!! | TTCATTTTTGGTGCTTAAGT+GGG | + | chr4.1:16120334-16120353 | None:intergenic | 30.0% |
| AATCTTGCCATCATAGACAA+TGG | + | chr4.1:16118985-16119004 | None:intergenic | 35.0% | |
| AATTGTATATATTGCAGGCC+AGG | - | chr4.1:16120913-16120932 | MS.gene001558:intron | 35.0% | |
| AGTTTAAAAACTACTCCCTC+CGG | + | chr4.1:16119243-16119262 | None:intergenic | 35.0% | |
| CACAAGAATTAAAGAGAGAG+AGG | + | chr4.1:16120133-16120152 | None:intergenic | 35.0% | |
| CATTGTGGAAAGAAAATGCT+TGG | - | chr4.1:16120076-16120095 | MS.gene001558:intron | 35.0% | |
| CTCTAAATCAACTACCTACA+AGG | + | chr4.1:16119725-16119744 | None:intergenic | 35.0% | |
| CTCTCTCTCTTTAATTCTTG+TGG | - | chr4.1:16120131-16120150 | MS.gene001558:intron | 35.0% | |
| CTTGAATGAAAGTTTGGTTG+AGG | + | chr4.1:16119755-16119774 | None:intergenic | 35.0% | |
| GAAAGAAGAAGAAGAAGAAG+AGG | - | chr4.1:16118915-16118934 | MS.gene001558:CDS | 35.0% | |
| TCTCTTGAGTGATCACAATA+TGG | + | chr4.1:16120830-16120849 | None:intergenic | 35.0% | |
| TGAGAGCTTGAATGAAAGTT+TGG | + | chr4.1:16119761-16119780 | None:intergenic | 35.0% | |
| TTCTCCAAAAGAGGAAGAAT+CGG | - | chr4.1:16121069-16121088 | MS.gene001558:CDS | 35.0% | |
| ! | AGCATTTTCTTTCCACAATG+GGG | + | chr4.1:16120076-16120095 | None:intergenic | 35.0% |
| ! | ATAGTTGTGCCCAAATTTTG+AGG | + | chr4.1:16120630-16120649 | None:intergenic | 35.0% |
| ! | CAAGCATTTTCTTTCCACAA+TGG | + | chr4.1:16120078-16120097 | None:intergenic | 35.0% |
| ! | CTTGTAGGTAGTTGATTTAG+AGG | - | chr4.1:16119723-16119742 | MS.gene001558:intron | 35.0% |
| !!! | CTTCATTTTTGGTGCTTAAG+TGG | + | chr4.1:16120335-16120354 | None:intergenic | 35.0% |
| !!! | TTAATTTTTTCTGCGGTGCT+AGG | - | chr4.1:16120234-16120253 | MS.gene001558:intron | 35.0% |
| AAGATCATAAGCAGCAGTAC+AGG | - | chr4.1:16118952-16118971 | MS.gene001558:CDS | 40.0% | |
| ACAAATGGATGCAGTGCATT+TGG | + | chr4.1:16120604-16120623 | None:intergenic | 40.0% | |
| ACAATAATGCATACTCCCTC+CGG | - | chr4.1:16119337-16119356 | MS.gene001558:intron | 40.0% | |
| AGCATCATCATCTAGTTAGG+AGG | - | chr4.1:16120758-16120777 | MS.gene001558:intron | 40.0% | |
| ATTAATAAGCACCCCCATTG+TGG | - | chr4.1:16120061-16120080 | MS.gene001558:intron | 40.0% | |
| CCAACTTCACTTCATTCACA+AGG | + | chr4.1:16120573-16120592 | None:intergenic | 40.0% | |
| CCATCATAGACAATGGTCAA+CGG | + | chr4.1:16118978-16118997 | None:intergenic | 40.0% | |
| CCTTGTGAATGAAGTGAAGT+TGG | - | chr4.1:16120570-16120589 | MS.gene001558:intron | 40.0% | |
| GAGAACAGATCTCTCCAAAA+CGG | + | chr4.1:16119703-16119722 | None:intergenic | 40.0% | |
| TCAAGGAGAGTTTGATCAAG+AGG | - | chr4.1:16119875-16119894 | MS.gene001558:intron | 40.0% | |
| TGCAAGCATTAAAATGGACC+TGG | + | chr4.1:16120934-16120953 | None:intergenic | 40.0% | |
| TGCATATAAATGTGACCGGA+GGG | - | chr4.1:16119225-16119244 | MS.gene001558:intron | 40.0% | |
| TGCTTATAAATGTGACCGGA+GGG | + | chr4.1:16119355-16119374 | None:intergenic | 40.0% | |
| TTGCATATAAATGTGACCGG+AGG | - | chr4.1:16119224-16119243 | MS.gene001558:intron | 40.0% | |
| TTGCTTATAAATGTGACCGG+AGG | + | chr4.1:16119356-16119375 | None:intergenic | 40.0% | |
| ! | AGTTAGAGCATTCACAATGG+TGG | - | chr4.1:16120014-16120033 | MS.gene001558:intron | 40.0% |
| ! | GAATCCGATTCTTCCTCTTT+TGG | + | chr4.1:16121076-16121095 | None:intergenic | 40.0% |
| ! | GCATTTTCTTTCCACAATGG+GGG | + | chr4.1:16120075-16120094 | None:intergenic | 40.0% |
| ! | GTTAGAGCATTCACAATGGT+GGG | - | chr4.1:16120015-16120034 | MS.gene001558:intron | 40.0% |
| ! | TGCTGCTTATGATCTTCTTG+TGG | + | chr4.1:16118948-16118967 | None:intergenic | 40.0% |
| ! | TTGGTGCTTACAGAGAAATG+TGG | - | chr4.1:16120095-16120114 | MS.gene001558:intron | 40.0% |
| !! | ATGGGGGTGCTTATTAATTC+TGG | + | chr4.1:16120059-16120078 | None:intergenic | 40.0% |
| !! | GCATGGAAAGAGAAGTACTA+GGG | + | chr4.1:16121031-16121050 | None:intergenic | 40.0% |
| !! | TGCATGGAAAGAGAAGTACT+AGG | + | chr4.1:16121032-16121051 | None:intergenic | 40.0% |
| !!! | AACTTTTGGTGACGTTCAAG+AGG | + | chr4.1:16119669-16119688 | None:intergenic | 40.0% |
| CCATGCAAAAATCGTTGCAG+AGG | - | chr4.1:16121045-16121064 | MS.gene001558:CDS | 45.0% | |
| CCGTTGACCATTGTCTATGA+TGG | - | chr4.1:16118975-16118994 | MS.gene001558:CDS | 45.0% | |
| GAGAGATCTGTTCTCCTTGT+AGG | - | chr4.1:16119708-16119727 | MS.gene001558:intron | 45.0% | |
| TGAACACACCTGAATCTCAG+TGG | + | chr4.1:16119021-16119040 | None:intergenic | 45.0% | |
| TGCAGAGGTTTCTCCAAAAG+AGG | - | chr4.1:16121060-16121079 | MS.gene001558:CDS | 45.0% | |
| TGCAGGTCTTACTACACATG+TGG | - | chr4.1:16119566-16119585 | MS.gene001558:intron | 45.0% | |
| ! | CCTCTGCAACGATTTTTGCA+TGG | + | chr4.1:16121048-16121067 | None:intergenic | 45.0% |
| !!! | TTTTTTCTGCGGTGCTAGGT+TGG | - | chr4.1:16120238-16120257 | MS.gene001558:intron | 45.0% |
| GAAATGGTGAGGACACCAAC+TGG | - | chr4.1:16120960-16120979 | MS.gene001558:intron | 50.0% | |
| GACTGATGCCACTGAGATTC+AGG | - | chr4.1:16119010-16119029 | MS.gene001558:CDS | 50.0% | |
| ! | CGGTGCTAGGTTGGAGAAAT+TGG | - | chr4.1:16120247-16120266 | MS.gene001558:intron | 50.0% |
| ! | GGCTCTGATGATGATCCAGT+TGG | + | chr4.1:16120978-16120997 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 16118912 | 16121139 | 16118912 | ID=MS.gene001558 |
| chr4.1 | mRNA | 16118912 | 16121139 | 16118912 | ID=MS.gene001558.t1;Parent=MS.gene001558 |
| chr4.1 | exon | 16121020 | 16121139 | 16121020 | ID=MS.gene001558.t1.exon1;Parent=MS.gene001558.t1 |
| chr4.1 | CDS | 16121020 | 16121139 | 16121020 | ID=cds.MS.gene001558.t1;Parent=MS.gene001558.t1 |
| chr4.1 | exon | 16118912 | 16119121 | 16118912 | ID=MS.gene001558.t1.exon2;Parent=MS.gene001558.t1 |
| chr4.1 | CDS | 16118912 | 16119121 | 16118912 | ID=cds.MS.gene001558.t1;Parent=MS.gene001558.t1 |
| Gene Sequence |
| Protein sequence |
