Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001569.t1 | XP_003615824.1 | 95.4 | 217 | 10 | 0 | 1 | 217 | 1 | 217 | 1.10E-104 | 389.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001569.t1 | Q9LU68 | 53.8 | 91 | 40 | 2 | 126 | 216 | 337 | 425 | 2.3e-15 | 84.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001569.t1 | B7FH76 | 95.4 | 217 | 10 | 0 | 1 | 217 | 1 | 217 | 7.7e-105 | 389.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene001569.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001569.t1 | MTR_5g072780 | 95.392 | 217 | 10 | 0 | 1 | 217 | 1 | 217 | 1.71e-148 | 412 |
| MS.gene001569.t1 | MTR_7g032240 | 39.850 | 133 | 41 | 3 | 123 | 216 | 219 | 351 | 2.72e-20 | 87.8 |
| MS.gene001569.t1 | MTR_1g110870 | 44.144 | 111 | 42 | 3 | 124 | 216 | 316 | 424 | 1.49e-19 | 86.3 |
| MS.gene001569.t1 | MTR_4g127420 | 53.488 | 86 | 36 | 2 | 129 | 210 | 293 | 378 | 3.39e-19 | 85.1 |
| MS.gene001569.t1 | MTR_8g104190 | 41.727 | 139 | 53 | 4 | 94 | 216 | 274 | 400 | 2.69e-18 | 82.8 |
| MS.gene001569.t1 | MTR_1g013450 | 36.441 | 118 | 62 | 3 | 106 | 216 | 186 | 297 | 1.30e-13 | 68.9 |
| MS.gene001569.t1 | MTR_3g105710 | 67.442 | 43 | 14 | 0 | 174 | 216 | 232 | 274 | 1.95e-11 | 62.4 |
| MS.gene001569.t1 | MTR_4g128930 | 67.442 | 43 | 14 | 0 | 174 | 216 | 298 | 340 | 3.91e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001569.t1 | AT1G07050 | 67.000 | 100 | 26 | 3 | 119 | 216 | 99 | 193 | 3.16e-37 | 129 |
| MS.gene001569.t1 | AT1G07050 | 64.894 | 94 | 26 | 3 | 119 | 210 | 99 | 187 | 2.87e-32 | 116 |
| MS.gene001569.t1 | AT5G14370 | 53.211 | 109 | 36 | 3 | 123 | 216 | 228 | 336 | 2.95e-28 | 109 |
| MS.gene001569.t1 | AT4G25990 | 53.922 | 102 | 39 | 3 | 119 | 216 | 286 | 383 | 1.43e-23 | 97.4 |
| MS.gene001569.t1 | AT5G57180 | 53.846 | 91 | 40 | 2 | 126 | 216 | 337 | 425 | 6.70e-23 | 95.9 |
| MS.gene001569.t1 | AT1G73870 | 42.553 | 141 | 57 | 5 | 94 | 216 | 253 | 387 | 4.66e-21 | 90.5 |
| MS.gene001569.t1 | AT4G25990 | 47.009 | 117 | 39 | 4 | 119 | 216 | 286 | 398 | 8.08e-20 | 87.0 |
| MS.gene001569.t1 | AT4G25990 | 52.083 | 96 | 38 | 3 | 119 | 210 | 286 | 377 | 2.03e-19 | 85.9 |
| MS.gene001569.t1 | AT1G49130 | 52.747 | 91 | 34 | 3 | 128 | 216 | 230 | 313 | 2.04e-19 | 85.1 |
| MS.gene001569.t1 | AT1G49130 | 52.747 | 91 | 34 | 3 | 128 | 216 | 237 | 320 | 2.13e-19 | 85.1 |
| MS.gene001569.t1 | AT1G25440 | 46.429 | 112 | 42 | 4 | 123 | 216 | 292 | 403 | 4.38e-19 | 85.1 |
| MS.gene001569.t1 | AT1G68520 | 41.525 | 118 | 51 | 3 | 117 | 216 | 282 | 399 | 9.82e-19 | 84.0 |
| MS.gene001569.t1 | AT5G57180 | 51.765 | 85 | 39 | 2 | 126 | 210 | 337 | 419 | 1.11e-18 | 84.0 |
| MS.gene001569.t1 | AT2G24790 | 61.111 | 54 | 21 | 0 | 163 | 216 | 218 | 271 | 3.73e-13 | 67.4 |
| MS.gene001569.t1 | AT5G24930 | 53.226 | 62 | 29 | 0 | 155 | 216 | 276 | 337 | 5.38e-12 | 64.3 |
| MS.gene001569.t1 | AT5G57660 | 69.767 | 43 | 13 | 0 | 174 | 216 | 285 | 327 | 1.23e-11 | 63.5 |
Find 34 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCATGTTCTTCTTTCTT+TGG | 0.183818 | 5.4:+61549026 | None:intergenic |
| GGTCTGACCGTGGCTCTCTT+TGG | 0.244079 | 5.4:-61548644 | MS.gene001569:CDS |
| GCTCTCTCTTCTTCCAATAT+TGG | 0.250193 | 5.4:+61547841 | None:intergenic |
| TTTGAATTATCAAGAGGTTT+TGG | 0.259887 | 5.4:-61548673 | MS.gene001569:CDS |
| AAAAGTGTGACCTTGATTCT+TGG | 0.281388 | 5.4:+61547715 | None:intergenic |
| GAAGAGAAGCAAGTGTTCTT+AGG | 0.336563 | 5.4:-61547816 | MS.gene001569:CDS |
| GTCTGACCGTGGCTCTCTTT+GGG | 0.337400 | 5.4:-61548643 | MS.gene001569:CDS |
| GATGGGAGAAGTGCCAATAT+TGG | 0.355037 | 5.4:-61547854 | MS.gene001569:CDS |
| AGTGTAAGATTTGTTGAAGT+TGG | 0.374346 | 5.4:+61548977 | None:intergenic |
| ATAGTAGTAATGAGCTTCAT+TGG | 0.401933 | 5.4:-61548851 | MS.gene001569:CDS |
| CTCATCTTTCCCTTCATCAA+AGG | 0.415253 | 5.4:+61548803 | None:intergenic |
| TCATCTTTCCCTTCATCAAA+GGG | 0.428053 | 5.4:+61548804 | None:intergenic |
| AAGAGAGAAAATTACAATGA+TGG | 0.429279 | 5.4:-61548741 | MS.gene001569:CDS |
| CATAAGCAAATGCAATTATG+AGG | 0.471927 | 5.4:-61548778 | MS.gene001569:CDS |
| TGAGCTTCATTGGGATATCA+TGG | 0.472007 | 5.4:-61548841 | MS.gene001569:CDS |
| GAGGGATTTCCCTTTGATGA+AGG | 0.484035 | 5.4:-61548813 | MS.gene001569:CDS |
| ACTAACATAGGAGCCACTAT+TGG | 0.497136 | 5.4:+61548590 | None:intergenic |
| TAGTAGTAATGAGCTTCATT+GGG | 0.508412 | 5.4:-61548850 | MS.gene001569:CDS |
| AGGGATTTCCCTTTGATGAA+GGG | 0.520507 | 5.4:-61548812 | MS.gene001569:CDS |
| TCATTGGGATATCATGGAAT+GGG | 0.534580 | 5.4:-61548835 | MS.gene001569:CDS |
| TTCATTGGGATATCATGGAA+TGG | 0.539426 | 5.4:-61548836 | MS.gene001569:CDS |
| GGTATTTGAGTGGTAGCGTC+TGG | 0.540746 | 5.4:+61549002 | None:intergenic |
| TTTGAATTTGAATTATCAAG+AGG | 0.541289 | 5.4:-61548679 | MS.gene001569:CDS |
| AACATAGGAGCCACTATTGG+AGG | 0.541652 | 5.4:+61548593 | None:intergenic |
| CGCTTTGTGAAGAGACATTG+AGG | 0.551371 | 5.4:-61547587 | None:intergenic |
| TAAGATTTGTTGAAGTTGGA+TGG | 0.551571 | 5.4:+61548981 | None:intergenic |
| TGATAAAAGACCAAGAATCA+AGG | 0.578251 | 5.4:-61547725 | MS.gene001569:intron |
| ATAGGAGCCACTATTGGAGG+AGG | 0.606351 | 5.4:+61548596 | None:intergenic |
| TTGGGATATCATGGAATGGG+AGG | 0.609318 | 5.4:-61548832 | MS.gene001569:CDS |
| GAAGTTGGATGGTATTTGAG+TGG | 0.615620 | 5.4:+61548992 | None:intergenic |
| TGGGATATCATGGAATGGGA+GGG | 0.659236 | 5.4:-61548831 | MS.gene001569:CDS |
| CATTAGCCCAAAGAGAGCCA+CGG | 0.677020 | 5.4:+61548637 | None:intergenic |
| TTGGATGCATGGTCTGACCG+TGG | 0.694015 | 5.4:-61548654 | MS.gene001569:CDS |
| AATGGATGATGAAAAGAGTG+TGG | 0.704180 | 5.4:-61548709 | MS.gene001569:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAATAATATAGTTGTTATA+TGG | + | chr5.4:61548206-61548225 | None:intergenic | 10.0% |
| !!! | TAATTTTAAATAGTAACAAA+AGG | + | chr5.4:61548238-61548257 | None:intergenic | 10.0% |
| !! | TAAACAAAAAAGAAATTACT+AGG | + | chr5.4:61548768-61548787 | None:intergenic | 15.0% |
| !!! | AATTTCTTTTTTGTTTAGAT+GGG | - | chr5.4:61548771-61548790 | MS.gene001569:CDS | 15.0% |
| !!! | GTTTCTTTTTTTTTTTCATT+GGG | - | chr5.4:61548971-61548990 | MS.gene001569:CDS | 15.0% |
| !!! | TAATTTCTTTTTTGTTTAGA+TGG | - | chr5.4:61548770-61548789 | MS.gene001569:CDS | 15.0% |
| !!! | TATCAATTCTTATTCTTTTA+AGG | - | chr5.4:61548155-61548174 | MS.gene001569:intron | 15.0% |
| !!! | TGTTTCTTTTTTTTTTTCAT+TGG | - | chr5.4:61548970-61548989 | MS.gene001569:CDS | 15.0% |
| !! | ATATAACAACTTATCCAAAA+AGG | - | chr5.4:61548180-61548199 | MS.gene001569:intron | 20.0% |
| !! | TTTGAATTTGAATTATCAAG+AGG | - | chr5.4:61547963-61547982 | MS.gene001569:intron | 20.0% |
| !!! | AAATTACAATGATGGTTTTT+TGG | - | chr5.4:61547909-61547928 | MS.gene001569:intron | 20.0% |
| !!! | ATAACTAAATTTTTGGTGAT+AGG | - | chr5.4:61548519-61548538 | MS.gene001569:intron | 20.0% |
| !!! | GTAACATATTTAGTTCATTT+TGG | - | chr5.4:61548615-61548634 | MS.gene001569:CDS | 20.0% |
| !!! | TATGTTGATTTTTGTTTAGA+TGG | - | chr5.4:61548400-61548419 | MS.gene001569:intron | 20.0% |
| !!! | TTTGTCGATAACTAAATTTT+TGG | - | chr5.4:61548512-61548531 | MS.gene001569:intron | 20.0% |
| !!! | TTTGTGATATTTTTGATGTA+GGG | - | chr5.4:61549031-61549050 | MS.gene001569:CDS | 20.0% |
| !!! | TTTTGTGATATTTTTGATGT+AGG | - | chr5.4:61549030-61549049 | MS.gene001569:CDS | 20.0% |
| !!! | TTTTTGGATAAGTTGTTATA+TGG | + | chr5.4:61548181-61548200 | None:intergenic | 20.0% |
| ! | AAGAGAGAAAATTACAATGA+TGG | - | chr5.4:61547901-61547920 | MS.gene001569:intron | 25.0% |
| ! | ACATATATGAAAGTGAAGAT+TGG | + | chr5.4:61548087-61548106 | None:intergenic | 25.0% |
| ! | TATTACTTAATTGACACCAA+CGG | + | chr5.4:61548382-61548401 | None:intergenic | 25.0% |
| ! | TGGATAATGAATACTAACAT+AGG | + | chr5.4:61548067-61548086 | None:intergenic | 25.0% |
| ! | TTATTCGATCTATTCAATTG+TGG | - | chr5.4:61548483-61548502 | MS.gene001569:intron | 25.0% |
| !! | TTTGAATTATCAAGAGGTTT+TGG | - | chr5.4:61547969-61547988 | MS.gene001569:intron | 25.0% |
| AAAACCTATTATCCAGAAAG+CGG | + | chr5.4:61548444-61548463 | None:intergenic | 30.0% | |
| ACTTCATGTTCTTCTTTCTT+TGG | + | chr5.4:61547619-61547638 | None:intergenic | 30.0% | |
| ATAGTAGTAATGAGCTTCAT+TGG | - | chr5.4:61547791-61547810 | MS.gene001569:CDS | 30.0% | |
| ATTACTTAATTGACACCAAC+GGG | + | chr5.4:61548381-61548400 | None:intergenic | 30.0% | |
| CATAAGCAAATGCAATTATG+AGG | - | chr5.4:61547864-61547883 | MS.gene001569:intron | 30.0% | |
| TAGTAGTAATGAGCTTCATT+GGG | - | chr5.4:61547792-61547811 | MS.gene001569:CDS | 30.0% | |
| TGATAAAAGACCAAGAATCA+AGG | - | chr5.4:61548917-61548936 | MS.gene001569:CDS | 30.0% | |
| ! | CTACTATTACAAGTGTTTTG+TGG | + | chr5.4:61547778-61547797 | None:intergenic | 30.0% |
| ! | GCAAGAATAGTCATCAATTT+TGG | + | chr5.4:61548295-61548314 | None:intergenic | 30.0% |
| ! | TAGTTGTTATATGGCCTTTT+TGG | + | chr5.4:61548197-61548216 | None:intergenic | 30.0% |
| !! | AGTGTAAGATTTGTTGAAGT+TGG | + | chr5.4:61547668-61547687 | None:intergenic | 30.0% |
| !! | TAAGATTTGTTGAAGTTGGA+TGG | + | chr5.4:61547664-61547683 | None:intergenic | 30.0% |
| !!! | CAATGATGGTTTTTTGGAAA+TGG | - | chr5.4:61547915-61547934 | MS.gene001569:intron | 30.0% |
| AATGGATGATGAAAAGAGTG+TGG | - | chr5.4:61547933-61547952 | MS.gene001569:intron | 35.0% | |
| GATAGGTAATCTTTGATGCT+TGG | - | chr5.4:61548536-61548555 | MS.gene001569:intron | 35.0% | |
| TCATCTTTCCCTTCATCAAA+GGG | + | chr5.4:61547841-61547860 | None:intergenic | 35.0% | |
| TCATTGGGATATCATGGAAT+GGG | - | chr5.4:61547807-61547826 | MS.gene001569:CDS | 35.0% | |
| TTCATTGGGATATCATGGAA+TGG | - | chr5.4:61547806-61547825 | MS.gene001569:CDS | 35.0% | |
| ! | AAAAGTGTGACCTTGATTCT+TGG | + | chr5.4:61548930-61548949 | None:intergenic | 35.0% |
| ! | CACATAAAGATTTTACCCGT+TGG | - | chr5.4:61548363-61548382 | MS.gene001569:intron | 35.0% |
| !! | CAGCGTTTAATTTTCGAACT+TGG | + | chr5.4:61548899-61548918 | None:intergenic | 35.0% |
| ACTAACATAGGAGCCACTAT+TGG | + | chr5.4:61548055-61548074 | None:intergenic | 40.0% | |
| AGGGATTTCCCTTTGATGAA+GGG | - | chr5.4:61547830-61547849 | MS.gene001569:CDS | 40.0% | |
| CTCATCTTTCCCTTCATCAA+AGG | + | chr5.4:61547842-61547861 | None:intergenic | 40.0% | |
| GAAGAGAAGCAAGTGTTCTT+AGG | - | chr5.4:61548826-61548845 | MS.gene001569:CDS | 40.0% | |
| GAAGTTGGATGGTATTTGAG+TGG | + | chr5.4:61547653-61547672 | None:intergenic | 40.0% | |
| GCTCTCTCTTCTTCCAATAT+TGG | + | chr5.4:61548804-61548823 | None:intergenic | 40.0% | |
| TGAGCTTCATTGGGATATCA+TGG | - | chr5.4:61547801-61547820 | MS.gene001569:CDS | 40.0% | |
| TTGACCGCTTTCTGGATAAT+AGG | - | chr5.4:61548437-61548456 | MS.gene001569:intron | 40.0% | |
| ! | TCATTTTCCTCCTCCAATAG+TGG | - | chr5.4:61548039-61548058 | MS.gene001569:intron | 40.0% |
| !!! | ATCAAGAGGTTTTGGATGCA+TGG | - | chr5.4:61547977-61547996 | MS.gene001569:intron | 40.0% |
| AACATAGGAGCCACTATTGG+AGG | + | chr5.4:61548052-61548071 | None:intergenic | 45.0% | |
| AACTCTTGTTGACCGCTTTC+TGG | - | chr5.4:61548429-61548448 | MS.gene001569:intron | 45.0% | |
| GAGGGATTTCCCTTTGATGA+AGG | - | chr5.4:61547829-61547848 | MS.gene001569:CDS | 45.0% | |
| TGGGATATCATGGAATGGGA+GGG | - | chr5.4:61547811-61547830 | MS.gene001569:CDS | 45.0% | |
| TTGGGATATCATGGAATGGG+AGG | - | chr5.4:61547810-61547829 | MS.gene001569:CDS | 45.0% | |
| !! | GATGGGAGAAGTGCCAATAT+TGG | - | chr5.4:61548788-61548807 | MS.gene001569:CDS | 45.0% |
| ATAGGAGCCACTATTGGAGG+AGG | + | chr5.4:61548049-61548068 | None:intergenic | 50.0% | |
| CATTAGCCCAAAGAGAGCCA+CGG | + | chr5.4:61548008-61548027 | None:intergenic | 50.0% | |
| ! | GGTATTTGAGTGGTAGCGTC+TGG | + | chr5.4:61547643-61547662 | None:intergenic | 50.0% |
| GTCTGACCGTGGCTCTCTTT+GGG | - | chr5.4:61547999-61548018 | MS.gene001569:intron | 55.0% | |
| TTGGATGCATGGTCTGACCG+TGG | - | chr5.4:61547988-61548007 | MS.gene001569:intron | 55.0% | |
| GGTCTGACCGTGGCTCTCTT+TGG | - | chr5.4:61547998-61548017 | MS.gene001569:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 61547589 | 61549075 | 61547589 | ID=MS.gene001569 |
| chr5.4 | mRNA | 61547589 | 61549075 | 61547589 | ID=MS.gene001569.t1;Parent=MS.gene001569 |
| chr5.4 | exon | 61548596 | 61549075 | 61548596 | ID=MS.gene001569.t1.exon1;Parent=MS.gene001569.t1 |
| chr5.4 | CDS | 61548596 | 61549075 | 61548596 | ID=cds.MS.gene001569.t1;Parent=MS.gene001569.t1 |
| chr5.4 | exon | 61547726 | 61547875 | 61547726 | ID=MS.gene001569.t1.exon2;Parent=MS.gene001569.t1 |
| chr5.4 | CDS | 61547726 | 61547875 | 61547726 | ID=cds.MS.gene001569.t1;Parent=MS.gene001569.t1 |
| chr5.4 | exon | 61547589 | 61547612 | 61547589 | ID=MS.gene001569.t1.exon3;Parent=MS.gene001569.t1 |
| chr5.4 | CDS | 61547589 | 61547612 | 61547589 | ID=cds.MS.gene001569.t1;Parent=MS.gene001569.t1 |
| Gene Sequence |
| Protein sequence |
