AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00167

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00167.t1 MTR_2g034250 90.066 604 38 5 1 599 1 587 0.0 1088
MS.gene00167.t1 MTR_2g034260 83.500 600 84 5 1 599 1 586 0.0 1004
MS.gene00167.t1 MTR_2g034280 78.369 601 104 6 1 599 1 577 0.0 938
MS.gene00167.t1 MTR_7g109580 45.492 488 243 6 112 599 92 556 9.29e-137 410
MS.gene00167.t1 MTR_1g086970 44.732 503 246 10 103 599 4 480 2.52e-133 398
MS.gene00167.t1 MTR_3g065980 29.353 402 253 6 209 598 159 541 8.30e-55 195
MS.gene00167.t1 MTR_7g027190 30.446 404 251 10 208 597 286 673 1.01e-45 172
MS.gene00167.t1 MTR_8g442410 30.000 390 254 10 215 597 148 525 2.31e-43 163
MS.gene00167.t1 MTR_4g104020 29.156 391 257 9 215 597 142 520 1.32e-42 160
MS.gene00167.t1 MTR_4g064200 29.124 388 250 10 217 596 279 649 2.62e-41 159
MS.gene00167.t1 MTR_4g064150 29.367 395 256 9 212 596 351 732 4.60e-41 159
MS.gene00167.t1 MTR_7g074650 29.973 377 233 12 226 597 442 792 7.39e-39 153
MS.gene00167.t1 MTR_4g102790 28.052 385 256 10 223 599 293 664 1.89e-38 151
MS.gene00167.t1 MTR_2g097463 28.070 399 239 8 217 596 285 654 9.51e-38 149
MS.gene00167.t1 MTR_7g062120 27.621 391 264 7 213 597 363 740 1.12e-37 149
MS.gene00167.t1 MTR_2g097473 29.156 391 245 9 217 596 284 653 2.63e-37 147
MS.gene00167.t1 MTR_4g064160 26.076 395 255 7 217 596 311 683 4.87e-37 147
MS.gene00167.t1 MTR_2g097467 28.535 389 250 8 217 596 284 653 5.67e-37 146
MS.gene00167.t1 MTR_5g094450 27.577 388 259 8 219 597 158 532 9.73e-37 144
MS.gene00167.t1 MTR_4g133660 25.907 386 267 7 217 596 170 542 1.88e-36 143
MS.gene00167.t1 MTR_4g064120 27.461 386 259 8 211 587 243 616 2.50e-36 144
MS.gene00167.t1 MTR_2g026250 26.615 387 265 7 217 597 223 596 4.51e-36 143
MS.gene00167.t1 MTR_2g097410 26.866 402 253 8 213 596 362 740 8.70e-36 143
MS.gene00167.t1 MTR_3g056110 27.320 388 256 8 219 597 172 542 1.50e-35 141
MS.gene00167.t1 MTR_4g064180 26.823 384 264 6 211 587 243 616 7.07e-35 140
MS.gene00167.t1 MTR_5g097480 27.485 342 226 7 219 552 174 501 1.21e-34 138
MS.gene00167.t1 MTR_2g097390 25.000 388 272 6 214 596 267 640 4.20e-34 137
MS.gene00167.t1 MTR_3g022830 26.995 426 247 11 223 598 26 437 2.34e-33 133
MS.gene00167.t1 MTR_2g097350 25.515 388 270 6 214 596 266 639 2.45e-33 135
MS.gene00167.t1 MTR_2g097310 24.742 388 273 6 214 596 264 637 7.80e-33 134
MS.gene00167.t1 MTR_4g077760 26.166 386 258 9 219 597 190 555 2.95e-32 131
MS.gene00167.t1 MTR_3g072710 25.945 397 275 9 212 596 110 499 3.24e-32 130
MS.gene00167.t1 MTR_2g082090 26.302 384 263 8 219 596 209 578 8.69e-32 130
MS.gene00167.t1 MTR_4g076020 27.321 377 234 10 226 597 88 429 4.72e-31 126
MS.gene00167.t1 MTR_1g069725 26.650 409 266 13 192 596 84 462 9.43e-31 125
MS.gene00167.t1 MTR_3g089055 26.718 393 244 10 223 597 199 565 8.58e-30 124
MS.gene00167.t1 MTR_3g089055 26.718 393 244 10 223 597 204 570 1.01e-29 124
MS.gene00167.t1 MTR_1g029420 25.130 386 259 7 223 597 226 592 1.16e-29 124
MS.gene00167.t1 MTR_2g097380 25.549 364 223 9 238 596 240 560 8.11e-29 121
MS.gene00167.t1 MTR_1g096030 26.265 415 262 12 207 597 65 459 2.00e-28 119
MS.gene00167.t1 MTR_5g058860 25.926 405 265 11 211 596 108 496 4.67e-28 118
MS.gene00167.t1 MTR_4g097080 23.500 400 286 6 202 597 120 503 5.68e-25 109
MS.gene00167.t1 MTR_8g093070 27.340 406 241 16 209 596 126 495 1.27e-22 102
MS.gene00167.t1 MTR_7g057230 25.707 389 251 11 221 596 293 656 8.16e-22 100
MS.gene00167.t1 MTR_4g076140 35.099 151 97 1 447 597 320 469 8.54e-22 99.4
MS.gene00167.t1 MTR_4g076140 28.028 289 179 9 183 459 1 272 2.28e-18 88.6
MS.gene00167.t1 MTR_5g015490 24.031 387 271 8 223 597 115 490 1.87e-21 98.2
MS.gene00167.t1 MTR_5g009080 30.677 251 152 8 198 445 33 264 1.96e-21 98.2
MS.gene00167.t1 MTR_5g009080 32.237 152 102 1 446 597 328 478 4.78e-21 97.1
MS.gene00167.t1 MTR_6g047750 25.564 399 245 14 221 597 294 662 1.62e-18 90.1
MS.gene00167.t1 MTR_2g099110 26.460 291 195 8 223 508 75 351 2.97e-18 88.2
MS.gene00167.t1 MTR_4g026485 24.479 384 262 12 218 597 265 624 7.32e-18 87.8
MS.gene00167.t1 MTR_0092s0100 24.672 381 266 9 218 597 371 731 7.71e-18 87.8
MS.gene00167.t1 MTR_0092s0100 24.672 381 266 9 218 597 368 728 7.89e-18 87.8
MS.gene00167.t1 MTR_8g020840 19.554 404 288 9 215 598 163 549 1.15e-15 80.5
MS.gene00167.t1 MTR_8g077940 24.432 352 236 11 221 564 181 510 1.43e-12 70.9
MS.gene00167.t1 MTR_2g089100 20.414 338 227 8 223 530 53 378 6.06e-12 68.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00167.t1 AT3G03450 30.789 393 253 7 209 598 170 546 2.26e-56 199
MS.gene00167.t1 AT2G01570 29.847 392 253 6 209 598 211 582 1.21e-54 195
MS.gene00167.t1 AT5G17490 29.820 389 248 8 213 598 151 517 1.44e-52 188
MS.gene00167.t1 AT1G14920 29.847 392 253 6 209 598 159 530 2.50e-52 188
MS.gene00167.t1 AT1G66350 28.535 389 252 6 211 598 144 507 4.12e-51 184
MS.gene00167.t1 AT1G07530 28.680 394 261 6 210 596 384 764 3.69e-43 166
MS.gene00167.t1 AT5G41920 30.280 393 248 12 209 597 30 400 3.73e-43 160
MS.gene00167.t1 AT3G46600 30.709 381 244 8 223 597 5 371 4.64e-43 159
MS.gene00167.t1 AT3G46600 30.334 389 251 8 215 597 205 579 1.57e-42 161
MS.gene00167.t1 AT5G59450 31.122 392 244 11 217 597 222 598 4.19e-42 160
MS.gene00167.t1 AT3G46600 30.334 389 251 8 215 597 173 547 4.59e-42 160
MS.gene00167.t1 AT1G55580 29.425 452 275 11 181 597 3 445 4.94e-40 152
MS.gene00167.t1 AT2G29060 28.133 391 261 7 214 596 276 654 8.59e-40 154
MS.gene00167.t1 AT2G29060 28.133 391 261 7 214 596 313 691 1.08e-39 155
MS.gene00167.t1 AT4G17230 27.461 386 258 8 219 597 155 525 1.10e-39 152
MS.gene00167.t1 AT4G17230 27.461 386 258 8 219 597 155 525 1.10e-39 152
MS.gene00167.t1 AT3G54220 29.839 372 241 9 226 596 297 649 5.18e-39 152
MS.gene00167.t1 AT2G29065 27.225 382 263 7 223 596 253 627 1.18e-38 151
MS.gene00167.t1 AT1G07520 25.806 403 271 8 208 596 140 528 2.25e-37 146
MS.gene00167.t1 AT1G07520 25.806 403 271 8 208 596 140 528 2.25e-37 146
MS.gene00167.t1 AT1G07520 25.806 403 271 8 208 596 304 692 5.17e-37 147
MS.gene00167.t1 AT1G07520 25.806 403 271 8 208 596 305 693 6.10e-37 146
MS.gene00167.t1 AT1G63100 26.566 399 263 9 214 596 244 628 7.28e-36 143
MS.gene00167.t1 AT1G63100 26.566 399 263 9 214 596 269 653 8.02e-36 143
MS.gene00167.t1 AT5G66770 27.318 399 261 10 210 597 204 584 8.52e-35 139
MS.gene00167.t1 AT2G37650 28.070 399 250 11 213 597 326 701 5.68e-34 138
MS.gene00167.t1 AT2G37650 28.070 399 250 11 213 597 338 713 6.47e-34 137
MS.gene00167.t1 AT5G48150 26.946 334 226 6 219 547 119 439 1.70e-33 134
MS.gene00167.t1 AT5G48150 26.946 334 226 6 219 547 119 439 1.70e-33 134
MS.gene00167.t1 AT5G48150 26.946 334 226 6 219 547 119 439 1.70e-33 134
MS.gene00167.t1 AT5G48150 26.946 334 226 6 219 547 119 439 1.70e-33 134
MS.gene00167.t1 AT1G50600 26.632 383 257 8 223 597 160 526 9.81e-33 132
MS.gene00167.t1 AT1G50600 26.632 383 257 8 223 597 231 597 2.73e-32 132
MS.gene00167.t1 AT1G50600 26.632 383 257 8 223 597 235 601 3.25e-32 132
MS.gene00167.t1 AT2G04890 24.802 379 262 7 223 597 54 413 3.21e-31 126
MS.gene00167.t1 AT3G50650 27.671 365 238 9 242 597 195 542 1.15e-30 126
MS.gene00167.t1 AT4G08250 26.238 404 273 12 203 596 88 476 1.36e-27 117
MS.gene00167.t1 AT1G21450 26.836 354 230 9 214 554 214 551 1.89e-23 105
MS.gene00167.t1 AT1G50420 30.242 248 154 8 201 446 38 268 9.71e-23 102
MS.gene00167.t1 AT1G50420 31.646 158 97 3 445 597 328 479 9.10e-20 93.2
MS.gene00167.t1 AT2G45160 25.281 356 236 9 249 597 307 639 7.58e-20 94.0
MS.gene00167.t1 AT3G49950 24.427 393 262 11 223 596 31 407 2.46e-19 91.3
MS.gene00167.t1 AT4G00150 26.884 398 228 18 217 597 203 554 6.58e-19 90.9
MS.gene00167.t1 AT4G37650 21.649 388 286 7 223 597 147 529 2.06e-18 89.4
MS.gene00167.t1 AT5G52510 22.727 396 265 10 221 597 267 640 7.75e-17 84.7
MS.gene00167.t1 AT3G60630 24.918 305 207 8 304 597 329 622 8.36e-17 84.3
MS.gene00167.t1 AT4G36710 23.601 411 277 16 199 598 102 486 3.24e-16 82.0
MS.gene00167.t1 AT3G13840 24.315 292 191 10 220 498 145 419 6.12e-11 65.5

Find 80 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATCCAGTTAATAATTT+TGG 0.117714 2.1:+58434380 MS.gene00167:CDS
GTTCATCCAATGATACATTT+TGG 0.127093 2.1:+58433985 MS.gene00167:CDS
AACCATTACTATAGGATTTA+TGG 0.158396 2.1:-58435290 None:intergenic
AATGGTCATTGTCTTCTTAT+TGG 0.198735 2.1:+58435655 MS.gene00167:CDS
CACATGTGTACAGGCTTTAT+TGG 0.199976 2.1:+58434886 MS.gene00167:CDS
GAGTCTCTTTGCTCTAATTT+TGG 0.217371 2.1:+58434083 MS.gene00167:CDS
CTGTTTCTTTGTCGATTCTT+TGG 0.237536 2.1:-58434730 None:intergenic
CAAACAGAAACAGAATTAAT+TGG 0.259014 2.1:-58435691 None:intergenic
TTGATTCTTAATCACCCTTT+TGG 0.265653 2.1:+58434512 MS.gene00167:CDS
GTTGACATATTGAAGAATTA+TGG 0.268072 2.1:+58434314 MS.gene00167:CDS
AGATCCTTGCTAACCTCTTC+AGG 0.285294 2.1:-58434806 None:intergenic
AAAATTTCATAATCTTGATA+TGG 0.286476 2.1:-58434179 None:intergenic
GAAACGGTTCACAAAAGATT+TGG 0.292025 2.1:-58435341 None:intergenic
TAATGAACCACACGCTTAAC+CGG 0.323577 2.1:-58434689 None:intergenic
CAATTAATTCTGTTTCTGTT+TGG 0.330110 2.1:+58435692 MS.gene00167:CDS
ATAACTATATCGAACGAAAA+AGG 0.330493 2.1:-58435127 None:intergenic
TTTCATTTCATTATGCCCTT+TGG 0.334107 2.1:-58434012 None:intergenic
TCGTAAAGCGCGACCATGGC+TGG 0.353922 2.1:-58434833 None:intergenic
TCGATTGAAGTGCTTGCATT+AGG 0.355406 2.1:-58434979 None:intergenic
ATCAAACCAAAATTATTAAC+TGG 0.365703 2.1:-58434386 None:intergenic
GGTTTCCCAAAATGTATCAT+TGG 0.392828 2.1:-58433991 None:intergenic
GCTAAAGGAGTTTGGTAGAA+TGG 0.396074 2.1:-58434287 None:intergenic
TTCAATATTCACATGTGTAC+AGG 0.409275 2.1:+58434877 MS.gene00167:CDS
CAAAATTATTAACTGGATGA+AGG 0.416773 2.1:-58434379 None:intergenic
GTCATTGTCTTCTTATTGGA+TGG 0.423148 2.1:+58435659 MS.gene00167:CDS
CAAAGAATCGACAAAGAAAC+AGG 0.427647 2.1:+58434731 MS.gene00167:CDS
GGACACAGGTAAAAGATTGA+TGG 0.431879 2.1:+58435081 MS.gene00167:CDS
ATGATTGTTTCGAGTTTGTC+TGG 0.434303 2.1:-58435253 None:intergenic
GTCTTTGTATCAAGCTGAAT+TGG 0.435480 2.1:+58435588 MS.gene00167:CDS
GGAATGTAAAGATTGATGTT+TGG 0.438820 2.1:+58435515 MS.gene00167:CDS
TAATTCTTCAATATGTCAAC+TGG 0.440835 2.1:-58434311 None:intergenic
ATCCAACAATATGCAAAAGA+TGG 0.448011 2.1:+58434769 MS.gene00167:CDS
ATTGATCTCGAAATTCGAAA+AGG 0.450113 2.1:+58434941 MS.gene00167:CDS
TCGGCGACAACCATTACTAT+AGG 0.455276 2.1:-58435298 None:intergenic
CAACTGGTGCTAAAGGAGTT+TGG 0.455400 2.1:-58434295 None:intergenic
AGGAATATTGTGGCTGAAGA+AGG 0.463268 2.1:+58435475 MS.gene00167:CDS
TTTCATAATCTTGATATGGT+TGG 0.468199 2.1:-58434175 None:intergenic
AATATGTCAACTGGTGCTAA+AGG 0.468976 2.1:-58434302 None:intergenic
AAATTCGAAAAGGGTGTCAA+TGG 0.472001 2.1:+58434951 MS.gene00167:CDS
GAAGATATCATGAAGATAGC+TGG 0.473521 2.1:+58434443 MS.gene00167:CDS
TGTCTTCTTATTGGATGGAA+AGG 0.484516 2.1:+58435664 MS.gene00167:CDS
TCTTGCACATTTGATATGAA+TGG 0.485082 2.1:+58435637 MS.gene00167:CDS
TCTTCGTCGGAATCTGCATC+AGG 0.487554 2.1:+58434488 MS.gene00167:CDS
AGAAACTCTTCTTCTTGTGA+TGG 0.492366 2.1:-58434122 None:intergenic
AAAATTATTAACTGGATGAA+GGG 0.499177 2.1:-58434378 None:intergenic
ATCTTCGAAGACAGGTAACC+CGG 0.500315 2.1:+58434670 MS.gene00167:CDS
AATATTGAAACTTGACTGAA+TGG 0.501754 2.1:-58434863 None:intergenic
GAGTCTTTATCTTCGAAGAC+AGG 0.510353 2.1:+58434662 MS.gene00167:CDS
TTGATCTCGAAATTCGAAAA+GGG 0.518620 2.1:+58434942 MS.gene00167:CDS
CGTAAAGCGCGACCATGGCT+GGG 0.530803 2.1:-58434832 None:intergenic
AACCATAAATCCTATAGTAA+TGG 0.541375 2.1:+58435288 MS.gene00167:CDS
TTTGTATCAAGCTGAATTGG+TGG 0.548313 2.1:+58435591 MS.gene00167:CDS
ATTATGATTAGCTTCAATCT+CGG 0.557666 2.1:-58435317 None:intergenic
ATCTTTATTTGATCCTGAAG+AGG 0.563321 2.1:+58434793 MS.gene00167:CDS
GTTCAGTTTCAATATAGTTG+AGG 0.565047 2.1:+58433953 MS.gene00167:CDS
ATCTTCGTAAAGCGCGACCA+TGG 0.566646 2.1:-58434837 None:intergenic
TTTCACAAGATTTGGAATGG+TGG 0.567481 2.1:+58435546 MS.gene00167:CDS
CATACAATCATCATCTTCGT+CGG 0.575813 2.1:+58434475 MS.gene00167:CDS
AAGGAGCTGAAAGAAAGAGT+AGG 0.577054 2.1:+58435494 MS.gene00167:CDS
TCATGGTATTAGGAATATTG+TGG 0.583709 2.1:+58435465 MS.gene00167:CDS
ATTGAACCATGTCAAAGTGT+AGG 0.587676 2.1:-58434208 None:intergenic
TGATCCTGAAGAGGTTAGCA+AGG 0.599740 2.1:+58434802 MS.gene00167:CDS
AGGTTATTACCTGATGATGA+AGG 0.603318 2.1:+58434350 MS.gene00167:CDS
TGATAACCTACACTTTGACA+TGG 0.605899 2.1:+58434202 MS.gene00167:CDS
AATGAACCACACGCTTAACC+GGG 0.608506 2.1:-58434688 None:intergenic
TTAGCTTGTGCTGAGAAAGT+CGG 0.613798 2.1:+58434596 MS.gene00167:CDS
GTTATCTTTAGAAGCTCGAG+TGG 0.620777 2.1:-58435013 None:intergenic
CACTTCGAAGCATATAGTTG+AGG 0.629728 2.1:+58435060 MS.gene00167:CDS
AGGTAACCCGGTTAAGCGTG+TGG 0.631363 2.1:+58434682 MS.gene00167:CDS
TCATGAAGATAGCTGGAACA+AGG 0.635820 2.1:+58434450 MS.gene00167:CDS
ATATTGAAGAATTATGGCAA+AGG 0.640071 2.1:+58434320 MS.gene00167:CDS
AAGATAACAGCTATTGAGAG+CGG 0.640580 2.1:+58435028 MS.gene00167:CDS
CAAGGATCTAAACCCAGCCA+TGG 0.642505 2.1:+58434820 MS.gene00167:CDS
ACTCGAAACAATCATGAGAG+TGG 0.651400 2.1:+58435261 MS.gene00167:CDS
AAGCATATAGTTGAGGACAC+AGG 0.664800 2.1:+58435067 MS.gene00167:CDS
ATTATGGCAAAGGATTCAAG+AGG 0.672484 2.1:+58434330 MS.gene00167:CDS
GTGGCTAAAAGATTTCCTTG+TGG 0.676872 2.1:+58435610 MS.gene00167:CDS
GAAGATCTAGGAGACTACAA+TGG 0.683524 2.1:+58434056 MS.gene00167:CDS
AGATTCGGAAGAAACAGTCG+CGG 0.728949 2.1:+58435192 MS.gene00167:CDS
AATGTGCAAGAACATCCACA+AGG 0.743242 2.1:-58435625 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTTCATAATCTTGATA+TGG - chr2.1:58434182-58434201 None:intergenic 15.0%
!! AAAATTATTAACTGGATGAA+GGG - chr2.1:58434381-58434400 None:intergenic 20.0%
!! ATCAAACCAAAATTATTAAC+TGG - chr2.1:58434389-58434408 None:intergenic 20.0%
!!! ATGATACTTTTTTTTATCAC+TGG - chr2.1:58434153-58434172 None:intergenic 20.0%
! AACCATAAATCCTATAGTAA+TGG + chr2.1:58435288-58435307 MS.gene00167:CDS 25.0%
! AACCATTACTATAGGATTTA+TGG - chr2.1:58435293-58435312 None:intergenic 25.0%
! AATATTGAAACTTGACTGAA+TGG - chr2.1:58434866-58434885 None:intergenic 25.0%
! ATAACTATATCGAACGAAAA+AGG - chr2.1:58435130-58435149 None:intergenic 25.0%
! ATATTGAAGAATTATGGCAA+AGG + chr2.1:58434320-58434339 MS.gene00167:CDS 25.0%
! CAAAATTATTAACTGGATGA+AGG - chr2.1:58434382-58434401 None:intergenic 25.0%
! CAAACAGAAACAGAATTAAT+TGG - chr2.1:58435694-58435713 None:intergenic 25.0%
! GTTGACATATTGAAGAATTA+TGG + chr2.1:58434314-58434333 MS.gene00167:CDS 25.0%
! TAATTCTTCAATATGTCAAC+TGG - chr2.1:58434314-58434333 None:intergenic 25.0%
! TTTCATAATCTTGATATGGT+TGG - chr2.1:58434178-58434197 None:intergenic 25.0%
!! ATTTTTCACAAGATTTGGAA+TGG + chr2.1:58435543-58435562 MS.gene00167:CDS 25.0%
!! CTTCATCCAGTTAATAATTT+TGG + chr2.1:58434380-58434399 MS.gene00167:CDS 25.0%
!! TTCATCCAATGATACATTTT+GGG + chr2.1:58433986-58434005 MS.gene00167:CDS 25.0%
!!! AGATCTTTTCAAGATAGATT+CGG + chr2.1:58435177-58435196 MS.gene00167:CDS 25.0%
!!! ATTATGATTAGCTTCAATCT+CGG - chr2.1:58435320-58435339 None:intergenic 25.0%
!!! CAATTAATTCTGTTTCTGTT+TGG + chr2.1:58435692-58435711 MS.gene00167:CDS 25.0%
!!! TGTATTTTAGTCATGGTATT+AGG + chr2.1:58435455-58435474 MS.gene00167:CDS 25.0%
!!! TGTTTTGAGAATTGTATGAA+AGG + chr2.1:58435400-58435419 MS.gene00167:CDS 25.0%
AATGGTCATTGTCTTCTTAT+TGG + chr2.1:58435655-58435674 MS.gene00167:CDS 30.0%
ATCCAACAATATGCAAAAGA+TGG + chr2.1:58434769-58434788 MS.gene00167:CDS 30.0%
ATTGATCTCGAAATTCGAAA+AGG + chr2.1:58434941-58434960 MS.gene00167:CDS 30.0%
CAGAGAAACAAAAACCAAAA+GGG - chr2.1:58434529-58434548 None:intergenic 30.0%
GGAATGTAAAGATTGATGTT+TGG + chr2.1:58435515-58435534 MS.gene00167:CDS 30.0%
GTTCAGTTTCAATATAGTTG+AGG + chr2.1:58433953-58433972 MS.gene00167:CDS 30.0%
TCATGGTATTAGGAATATTG+TGG + chr2.1:58435465-58435484 MS.gene00167:CDS 30.0%
TCTTGCACATTTGATATGAA+TGG + chr2.1:58435637-58435656 MS.gene00167:CDS 30.0%
TTCAATATTCACATGTGTAC+AGG + chr2.1:58434877-58434896 MS.gene00167:CDS 30.0%
TTGATCTCGAAATTCGAAAA+GGG + chr2.1:58434942-58434961 MS.gene00167:CDS 30.0%
TTTCATTTCATTATGCCCTT+TGG - chr2.1:58434015-58434034 None:intergenic 30.0%
! ATCTTTATTTGATCCTGAAG+AGG + chr2.1:58434793-58434812 MS.gene00167:CDS 30.0%
! CAAAGTGGAAGAATTTTTGT+TGG - chr2.1:58434248-58434267 None:intergenic 30.0%
! GTTCATCCAATGATACATTT+TGG + chr2.1:58433985-58434004 MS.gene00167:CDS 30.0%
! TTCCATCTTTTGCATATTGT+TGG - chr2.1:58434774-58434793 None:intergenic 30.0%
! TTGATTCTTAATCACCCTTT+TGG + chr2.1:58434512-58434531 MS.gene00167:CDS 30.0%
!! AAAAAAGTGCTTCGATGAAA+CGG - chr2.1:58435360-58435379 None:intergenic 30.0%
!! AGAGCATTTTTCACAAGATT+TGG + chr2.1:58435538-58435557 MS.gene00167:CDS 30.0%
!! ATACATTTTGGGAAACCAAA+GGG + chr2.1:58433997-58434016 MS.gene00167:CDS 30.0%
!!! GAGTCAATGTATTTTAGTCA+TGG + chr2.1:58435448-58435467 MS.gene00167:CDS 30.0%
AAGATAACAGCTATTGAGAG+CGG + chr2.1:58435028-58435047 MS.gene00167:CDS 35.0%
AGAAACTCTTCTTCTTGTGA+TGG - chr2.1:58434125-58434144 None:intergenic 35.0%
ATTATGGCAAAGGATTCAAG+AGG + chr2.1:58434330-58434349 MS.gene00167:CDS 35.0%
ATTGAACCATGTCAAAGTGT+AGG - chr2.1:58434211-58434230 None:intergenic 35.0%
CAAAGAATCGACAAAGAAAC+AGG + chr2.1:58434731-58434750 MS.gene00167:CDS 35.0%
CATACAATCATCATCTTCGT+CGG + chr2.1:58434475-58434494 MS.gene00167:CDS 35.0%
CCAGAGAAACAAAAACCAAA+AGG - chr2.1:58434530-58434549 None:intergenic 35.0%
GAAACGGTTCACAAAAGATT+TGG - chr2.1:58435344-58435363 None:intergenic 35.0%
GAAGATATCATGAAGATAGC+TGG + chr2.1:58434443-58434462 MS.gene00167:CDS 35.0%
GGTTTCCCAAAATGTATCAT+TGG - chr2.1:58433994-58434013 None:intergenic 35.0%
GTCATTGTCTTCTTATTGGA+TGG + chr2.1:58435659-58435678 MS.gene00167:CDS 35.0%
GTCTTTGTATCAAGCTGAAT+TGG + chr2.1:58435588-58435607 MS.gene00167:CDS 35.0%
TCCACTTTGTGACACAAAAA+AGG + chr2.1:58434259-58434278 MS.gene00167:CDS 35.0%
TGATAACCTACACTTTGACA+TGG + chr2.1:58434202-58434221 MS.gene00167:CDS 35.0%
TGTCTTCTTATTGGATGGAA+AGG + chr2.1:58435664-58435683 MS.gene00167:CDS 35.0%
TTTGTATCAAGCTGAATTGG+TGG + chr2.1:58435591-58435610 MS.gene00167:CDS 35.0%
! AATATGTCAACTGGTGCTAA+AGG - chr2.1:58434305-58434324 None:intergenic 35.0%
! AGGTTATTACCTGATGATGA+AGG + chr2.1:58434350-58434369 MS.gene00167:CDS 35.0%
! GAGTCTCTTTGCTCTAATTT+TGG + chr2.1:58434083-58434102 MS.gene00167:CDS 35.0%
! TTTCACAAGATTTGGAATGG+TGG + chr2.1:58435546-58435565 MS.gene00167:CDS 35.0%
! TTTTCTGTAGCAGAAGATCT+AGG + chr2.1:58434044-58434063 MS.gene00167:CDS 35.0%
!! AAATTCGAAAAGGGTGTCAA+TGG + chr2.1:58434951-58434970 MS.gene00167:CDS 35.0%
!! ATGATTGTTTCGAGTTTGTC+TGG - chr2.1:58435256-58435275 None:intergenic 35.0%
!! CTGTTTCTTTGTCGATTCTT+TGG - chr2.1:58434733-58434752 None:intergenic 35.0%
!! GATACATTTTGGGAAACCAA+AGG + chr2.1:58433996-58434015 MS.gene00167:CDS 35.0%
!!! CCTTTTGGTTTTTGTTTCTC+TGG + chr2.1:58434527-58434546 MS.gene00167:CDS 35.0%
!!! TCCTTTTTTGTGTCACAAAG+TGG - chr2.1:58434263-58434282 None:intergenic 35.0%
AAGCATATAGTTGAGGACAC+AGG + chr2.1:58435067-58435086 MS.gene00167:CDS 40.0%
AAGGAGCTGAAAGAAAGAGT+AGG + chr2.1:58435494-58435513 MS.gene00167:CDS 40.0%
AATGTGCAAGAACATCCACA+AGG - chr2.1:58435628-58435647 None:intergenic 40.0%
ACTCGAAACAATCATGAGAG+TGG + chr2.1:58435261-58435280 MS.gene00167:CDS 40.0%
AGGAATATTGTGGCTGAAGA+AGG + chr2.1:58435475-58435494 MS.gene00167:CDS 40.0%
CACTTCGAAGCATATAGTTG+AGG + chr2.1:58435060-58435079 MS.gene00167:CDS 40.0%
GAAGATCTAGGAGACTACAA+TGG + chr2.1:58434056-58434075 MS.gene00167:CDS 40.0%
GAGTCTTTATCTTCGAAGAC+AGG + chr2.1:58434662-58434681 MS.gene00167:CDS 40.0%
GCTAAAGGAGTTTGGTAGAA+TGG - chr2.1:58434290-58434309 None:intergenic 40.0%
GGACACAGGTAAAAGATTGA+TGG + chr2.1:58435081-58435100 MS.gene00167:CDS 40.0%
GTGGCTAAAAGATTTCCTTG+TGG + chr2.1:58435610-58435629 MS.gene00167:CDS 40.0%
TAATGAACCACACGCTTAAC+CGG - chr2.1:58434692-58434711 None:intergenic 40.0%
TCATGAAGATAGCTGGAACA+AGG + chr2.1:58434450-58434469 MS.gene00167:CDS 40.0%
! AGGGTTTTTCCTTCATCATC+AGG - chr2.1:58434362-58434381 None:intergenic 40.0%
! CACATGTGTACAGGCTTTAT+TGG + chr2.1:58434886-58434905 MS.gene00167:CDS 40.0%
! GTTATCTTTAGAAGCTCGAG+TGG - chr2.1:58435016-58435035 None:intergenic 40.0%
! TCGATTGAAGTGCTTGCATT+AGG - chr2.1:58434982-58435001 None:intergenic 40.0%
!! TTAGCTTGTGCTGAGAAAGT+CGG + chr2.1:58434596-58434615 MS.gene00167:CDS 40.0%
AATGAACCACACGCTTAACC+GGG - chr2.1:58434691-58434710 None:intergenic 45.0%
AGATCCTTGCTAACCTCTTC+AGG - chr2.1:58434809-58434828 None:intergenic 45.0%
AGATTCGGAAGAAACAGTCG+CGG + chr2.1:58435192-58435211 MS.gene00167:CDS 45.0%
ATCTTCGAAGACAGGTAACC+CGG + chr2.1:58434670-58434689 MS.gene00167:CDS 45.0%
TCGGCGACAACCATTACTAT+AGG - chr2.1:58435301-58435320 None:intergenic 45.0%
TGATCCTGAAGAGGTTAGCA+AGG + chr2.1:58434802-58434821 MS.gene00167:CDS 45.0%
! CAACTGGTGCTAAAGGAGTT+TGG - chr2.1:58434298-58434317 None:intergenic 45.0%
ATCTTCGTAAAGCGCGACCA+TGG - chr2.1:58434840-58434859 None:intergenic 50.0%
CAAGGATCTAAACCCAGCCA+TGG + chr2.1:58434820-58434839 MS.gene00167:CDS 50.0%
TCTTCGTCGGAATCTGCATC+AGG + chr2.1:58434488-58434507 MS.gene00167:CDS 50.0%
AGGTAACCCGGTTAAGCGTG+TGG + chr2.1:58434682-58434701 MS.gene00167:CDS 55.0%
CGTAAAGCGCGACCATGGCT+GGG - chr2.1:58434835-58434854 None:intergenic 60.0%
TCGTAAAGCGCGACCATGGC+TGG - chr2.1:58434836-58434855 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 58433927 58435726 58433927 ID=MS.gene00167
chr2.1 mRNA 58433927 58435726 58433927 ID=MS.gene00167.t1;Parent=MS.gene00167
chr2.1 exon 58433927 58435726 58433927 ID=MS.gene00167.t1.exon1;Parent=MS.gene00167.t1
chr2.1 CDS 58433927 58435726 58433927 ID=cds.MS.gene00167.t1;Parent=MS.gene00167.t1
Gene Sequence

>MS.gene00167

ATGGAAAACTTGTGCAATTTTGATGAGTTCAGTTTCAATATAGTTGAGGATAAACTTAGTTCATCCAATGATACATTTTGGGAAACCAAAGGGCATAATGAAATGAAAAATGTACAATTTTCTGTAGCAGAAGATCTAGGAGACTACAATGGAATTGAGTCTCTTTGCTCTAATTTTGGTTTCTTTCAAGATGATCCATCACAAGAAGAAGAGTTTCTTCTTTCCAGTGATAAAAAAAAGTATCATCACCAACCATATCAAGATTATGAAATTTTTGATAACCTACACTTTGACATGGTTCAATTTGATGAACAATGTCCAACAAAAATTCTTCCACTTTGTGACACAAAAAAGGACAAACCATTCTACCAAACTCCTTTAGCACCAGTTGACATATTGAAGAATTATGGCAAAGGATTCAAGAGGTTATTACCTGATGATGAAGGAAAAACCCTTCATCCAGTTAATAATTTTGGTTTGATCACAAATAATGAAAATGAGAGTAAGTTGTCAACTGAAGATATCATGAAGATAGCTGGAACAAGGTTCATACAATCATCATCTTCGTCGGAATCTGCATCAGGTTTGATTCTTAATCACCCTTTTGGTTTTTGTTTCTCTGGTTTATCTGATGAAGAGAAAGAAGATGTTTCACTTGCTGAATCACTCTTAGCTTGTGCTGAGAAAGTCGGATATCAACAATATGAACGCGCTAGAAAATTGTTGTCACAAATCGAGTCTTTATCTTCGAAGACAGGTAACCCGGTTAAGCGTGTGGTTCATTATTTTGCTGAAGCTCTTCGCCAAAGAATCGACAAAGAAACAGGACTATTTTCTGTTTCATCCAACAATATGCAAAAGATGGAATCTTTATTTGATCCTGAAGAGGTTAGCAAGGATCTAAACCCAGCCATGGTCGCGCTTTACGAAGATCTTCCATTCAGTCAAGTTTCAATATTCACATGTGTACAGGCTTTATTGGAAAATGTAAATGATGCAAAGAAGATTCATGTTATTGATCTCGAAATTCGAAAAGGGTGTCAATGGACAATCCTAATGCAAGCACTTCAATCGAGAAATGAATGTCCACTCGAGCTTCTAAAGATAACAGCTATTGAGAGCGGAAACTCAGACACTTCGAAGCATATAGTTGAGGACACAGGTAAAAGATTGATGGATTTTGCGCAAAGTATGAACATACCTTTTTCGTTCGATATAGTTATTGTATCTGATTTGTTAAATATAAGAGAAGATCTTTTCAAGATAGATTCGGAAGAAACAGTCGCGGTTTATTCTCAATTTGCGCTTAAAAGCAAGATTCAACAACCAGACAAACTCGAAACAATCATGAGAGTGGTTAGAACCATAAATCCTATAGTAATGGTTGTCGCCGAGATTGAAGCTAATCATAATTCCAAATCTTTTGTGAACCGTTTCATCGAAGCACTTTTTTACTTTAGTGCATACTTTGATTGTTTTGAGAATTGTATGAAAGGTGATGAGAAAAACAGATTTATTCTAGAGTCAATGTATTTTAGTCATGGTATTAGGAATATTGTGGCTGAAGAAGGAGCTGAAAGAAAGAGTAGGAATGTAAAGATTGATGTTTGGAGAGCATTTTTCACAAGATTTGGAATGGTGGAAACAGAATTGAGTATGAAGTCTTTGTATCAAGCTGAATTGGTGGCTAAAAGATTTCCTTGTGGATGTTCTTGCACATTTGATATGAATGGTCATTGTCTTCTTATTGGATGGAAAGGAACACCAATTAATTCTGTTTCTGTTTGGAAATTCATTTGA
Protein sequence

>MS.gene00167.t1

MENLCNFDEFSFNIVEDKLSSSNDTFWETKGHNEMKNVQFSVAEDLGDYNGIESLCSNFGFFQDDPSQEEEFLLSSDKKKYHHQPYQDYEIFDNLHFDMVQFDEQCPTKILPLCDTKKDKPFYQTPLAPVDILKNYGKGFKRLLPDDEGKTLHPVNNFGLITNNENESKLSTEDIMKIAGTRFIQSSSSSESASGLILNHPFGFCFSGLSDEEKEDVSLAESLLACAEKVGYQQYERARKLLSQIESLSSKTGNPVKRVVHYFAEALRQRIDKETGLFSVSSNNMQKMESLFDPEEVSKDLNPAMVALYEDLPFSQVSIFTCVQALLENVNDAKKIHVIDLEIRKGCQWTILMQALQSRNECPLELLKITAIESGNSDTSKHIVEDTGKRLMDFAQSMNIPFSFDIVIVSDLLNIREDLFKIDSEETVAVYSQFALKSKIQQPDKLETIMRVVRTINPIVMVVAEIEANHNSKSFVNRFIEALFYFSAYFDCFENCMKGDEKNRFILESMYFSHGIRNIVAEEGAERKSRNVKIDVWRAFFTRFGMVETELSMKSLYQAELVAKRFPCGCSCTFDMNGHCLLIGWKGTPINSVSVWKFI