AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00168

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00168.t1 MTR_2g034260 88.870 602 45 5 1 596 1 586 0.0 1076
MS.gene00168.t1 MTR_2g034250 84.307 599 79 4 1 596 1 587 0.0 1018
MS.gene00168.t1 MTR_2g034280 77.446 603 103 6 1 596 1 577 0.0 927
MS.gene00168.t1 MTR_7g109580 43.164 512 256 8 91 596 74 556 7.10e-134 402
MS.gene00168.t1 MTR_1g086970 43.825 502 251 10 101 596 4 480 9.74e-130 389
MS.gene00168.t1 MTR_3g065980 28.465 404 254 6 206 595 159 541 4.97e-54 193
MS.gene00168.t1 MTR_7g027190 30.221 407 248 11 205 594 286 673 2.38e-47 176
MS.gene00168.t1 MTR_4g064150 28.822 399 253 9 209 593 351 732 8.06e-44 167
MS.gene00168.t1 MTR_4g104020 28.718 390 260 9 212 594 142 520 2.37e-43 162
MS.gene00168.t1 MTR_8g442410 29.592 392 253 9 212 594 148 525 2.67e-42 160
MS.gene00168.t1 MTR_4g064200 29.668 391 244 12 214 593 279 649 1.56e-41 159
MS.gene00168.t1 MTR_4g076140 26.749 486 268 11 180 594 1 469 9.04e-40 152
MS.gene00168.t1 MTR_2g097463 27.907 387 255 7 214 593 285 654 3.67e-39 152
MS.gene00168.t1 MTR_2g097473 29.563 389 246 8 214 593 284 653 4.75e-39 152
MS.gene00168.t1 MTR_7g062120 28.061 392 261 8 210 594 363 740 4.89e-39 153
MS.gene00168.t1 MTR_4g064120 27.202 386 260 8 208 584 243 616 1.56e-38 150
MS.gene00168.t1 MTR_7g074650 30.400 375 234 10 223 594 442 792 4.89e-38 150
MS.gene00168.t1 MTR_4g102790 28.021 389 259 10 216 596 289 664 1.02e-37 149
MS.gene00168.t1 MTR_2g097467 28.424 387 253 7 214 593 284 653 1.29e-37 148
MS.gene00168.t1 MTR_4g064180 27.835 388 255 9 208 584 243 616 1.33e-37 148
MS.gene00168.t1 MTR_4g064160 26.753 385 265 6 214 593 311 683 8.47e-37 146
MS.gene00168.t1 MTR_5g094450 26.735 389 261 8 216 594 158 532 5.14e-36 142
MS.gene00168.t1 MTR_2g097410 27.296 392 264 9 210 593 362 740 8.57e-36 143
MS.gene00168.t1 MTR_4g133660 24.870 386 271 7 214 593 170 542 4.11e-35 139
MS.gene00168.t1 MTR_3g022830 27.739 429 240 8 220 595 26 437 4.93e-35 137
MS.gene00168.t1 MTR_1g069725 26.961 408 266 11 189 593 84 462 1.86e-34 136
MS.gene00168.t1 MTR_4g077760 26.042 384 261 8 216 594 190 555 2.75e-34 137
MS.gene00168.t1 MTR_4g076020 28.723 376 230 9 223 594 88 429 1.55e-33 133
MS.gene00168.t1 MTR_2g097350 24.485 388 274 6 211 593 266 639 4.10e-33 134
MS.gene00168.t1 MTR_2g026250 24.806 387 272 8 214 594 223 596 7.17e-33 134
MS.gene00168.t1 MTR_2g097390 23.711 388 277 6 211 593 267 640 7.37e-33 134
MS.gene00168.t1 MTR_2g082090 24.952 521 337 15 110 593 75 578 1.27e-32 132
MS.gene00168.t1 MTR_5g097480 26.176 340 229 7 220 551 178 503 1.50e-32 132
MS.gene00168.t1 MTR_2g097310 23.196 388 279 6 211 593 264 637 2.09e-32 132
MS.gene00168.t1 MTR_3g072710 26.980 404 262 13 209 593 110 499 6.18e-31 127
MS.gene00168.t1 MTR_3g056110 24.352 386 270 7 216 594 172 542 1.38e-30 126
MS.gene00168.t1 MTR_1g029420 25.450 389 254 9 220 594 226 592 1.33e-29 124
MS.gene00168.t1 MTR_3g089055 28.448 348 207 10 220 549 204 527 2.12e-29 123
MS.gene00168.t1 MTR_3g089055 27.907 344 214 9 220 549 199 522 2.35e-29 122
MS.gene00168.t1 MTR_5g058860 26.150 413 266 12 204 593 100 496 1.70e-27 116
MS.gene00168.t1 MTR_2g097380 23.901 364 229 9 235 593 240 560 1.79e-27 117
MS.gene00168.t1 MTR_1g096030 24.641 418 265 13 204 594 65 459 7.57e-27 114
MS.gene00168.t1 MTR_4g097080 24.060 399 285 6 199 594 120 503 1.91e-26 113
MS.gene00168.t1 MTR_8g093070 27.681 401 246 15 206 593 126 495 1.96e-26 113
MS.gene00168.t1 MTR_7g057230 25.714 385 256 9 218 593 293 656 1.97e-23 105
MS.gene00168.t1 MTR_5g009080 28.226 248 162 6 195 442 33 264 2.07e-22 101
MS.gene00168.t1 MTR_5g009080 32.237 152 102 1 443 594 328 478 1.16e-21 99.0
MS.gene00168.t1 MTR_5g015490 24.806 387 268 9 220 594 115 490 2.61e-22 100
MS.gene00168.t1 MTR_4g026485 25.455 385 257 11 215 594 265 624 4.95e-20 94.7
MS.gene00168.t1 MTR_2g099110 24.913 289 202 6 220 505 75 351 2.03e-19 91.7
MS.gene00168.t1 MTR_6g047750 29.710 276 164 9 335 594 401 662 3.44e-19 92.0
MS.gene00168.t1 MTR_0092s0100 23.196 388 263 10 215 594 368 728 8.80e-17 84.7
MS.gene00168.t1 MTR_0092s0100 23.196 388 263 10 215 594 371 731 9.15e-17 84.3
MS.gene00168.t1 MTR_2g089100 20.414 338 227 9 220 527 53 378 6.84e-14 74.7
MS.gene00168.t1 MTR_8g020840 19.802 404 287 10 212 595 163 549 1.27e-12 70.9
MS.gene00168.t1 MTR_8g077940 22.286 350 248 8 217 561 180 510 8.64e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00168.t1 AT3G03450 31.714 391 252 7 206 595 170 546 3.61e-61 212
MS.gene00168.t1 AT5G17490 30.591 389 245 8 210 595 151 517 2.41e-55 196
MS.gene00168.t1 AT2G01570 30.457 394 248 7 206 595 211 582 3.35e-55 197
MS.gene00168.t1 AT1G14920 30.280 393 250 7 206 595 159 530 5.59e-53 189
MS.gene00168.t1 AT1G66350 29.306 389 249 5 208 595 144 507 6.61e-53 189
MS.gene00168.t1 AT5G41920 30.730 397 241 12 206 594 30 400 7.64e-46 167
MS.gene00168.t1 AT5G59450 31.362 389 247 9 214 594 222 598 7.01e-45 169
MS.gene00168.t1 AT3G46600 30.709 381 244 8 220 594 5 371 1.74e-44 162
MS.gene00168.t1 AT3G46600 30.334 389 251 8 212 594 205 579 5.24e-44 166
MS.gene00168.t1 AT3G46600 30.334 389 251 8 212 594 173 547 1.45e-43 164
MS.gene00168.t1 AT1G07530 28.680 394 261 6 207 593 384 764 6.88e-42 162
MS.gene00168.t1 AT1G55580 28.889 450 280 8 178 594 3 445 8.30e-42 157
MS.gene00168.t1 AT2G29060 28.900 391 258 8 211 593 276 654 4.36e-41 158
MS.gene00168.t1 AT2G29060 28.900 391 258 8 211 593 313 691 5.66e-41 158
MS.gene00168.t1 AT2G29065 25.916 382 268 6 220 593 253 627 1.16e-40 157
MS.gene00168.t1 AT1G50420 26.240 484 266 13 183 594 15 479 1.72e-40 154
MS.gene00168.t1 AT3G54220 30.914 372 237 10 223 593 297 649 6.27e-39 152
MS.gene00168.t1 AT1G07520 24.685 397 283 6 205 593 140 528 2.74e-38 149
MS.gene00168.t1 AT1G07520 24.685 397 283 6 205 593 140 528 2.74e-38 149
MS.gene00168.t1 AT1G07520 24.685 397 283 6 205 593 305 693 6.10e-38 149
MS.gene00168.t1 AT1G07520 24.685 397 283 6 205 593 304 692 6.85e-38 149
MS.gene00168.t1 AT2G37650 29.442 394 251 10 210 594 326 701 1.48e-37 148
MS.gene00168.t1 AT2G37650 29.442 394 251 10 210 594 338 713 1.67e-37 148
MS.gene00168.t1 AT1G63100 26.065 399 265 8 211 593 269 653 6.34e-37 146
MS.gene00168.t1 AT1G63100 26.601 406 254 9 211 593 244 628 7.69e-37 145
MS.gene00168.t1 AT5G66770 27.456 397 263 10 207 594 204 584 9.72e-37 145
MS.gene00168.t1 AT4G17230 24.611 386 269 7 216 594 155 525 1.15e-34 138
MS.gene00168.t1 AT4G17230 24.611 386 269 7 216 594 155 525 1.15e-34 138
MS.gene00168.t1 AT2G04890 25.258 388 265 8 211 594 47 413 1.65e-32 130
MS.gene00168.t1 AT5G48150 23.711 388 271 8 216 594 119 490 1.02e-31 129
MS.gene00168.t1 AT5G48150 23.711 388 271 8 216 594 119 490 1.02e-31 129
MS.gene00168.t1 AT5G48150 23.711 388 271 8 216 594 119 490 1.02e-31 129
MS.gene00168.t1 AT5G48150 23.711 388 271 8 216 594 119 490 1.02e-31 129
MS.gene00168.t1 AT3G50650 27.248 367 237 9 239 594 195 542 2.55e-31 128
MS.gene00168.t1 AT1G50600 25.000 380 267 6 220 594 160 526 1.17e-30 126
MS.gene00168.t1 AT1G50600 25.000 380 267 6 220 594 231 597 2.56e-30 126
MS.gene00168.t1 AT1G50600 25.000 380 267 6 220 594 235 601 2.72e-30 126
MS.gene00168.t1 AT4G08250 24.878 410 271 13 200 593 88 476 6.73e-24 105
MS.gene00168.t1 AT1G21450 24.620 329 230 7 220 542 226 542 8.79e-22 100
MS.gene00168.t1 AT2G45160 24.304 395 260 11 216 594 268 639 7.63e-21 97.4
MS.gene00168.t1 AT4G37650 23.232 396 270 12 220 594 147 529 1.30e-19 92.8
MS.gene00168.t1 AT3G60630 25.574 305 205 9 301 594 329 622 2.38e-17 86.3
MS.gene00168.t1 AT4G00150 26.000 400 229 17 214 594 203 554 5.60e-17 84.7
MS.gene00168.t1 AT3G49950 24.181 397 258 13 220 593 31 407 5.95e-16 80.9
MS.gene00168.t1 AT4G36710 21.602 412 284 13 196 595 102 486 2.38e-14 76.3
MS.gene00168.t1 AT3G13840 25.086 291 193 8 208 488 137 412 8.20e-12 68.2

Find 82 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCATCCAGTTAATGATTT+TGG 0.114608 2.1:+58428837 MS.gene00168:CDS
AAGTCTCTTTGCACTAATTT+TGG 0.183092 2.1:+58428549 MS.gene00168:CDS
ATGGAAAACTTGTGCAATTT+TGG 0.214814 2.1:+58428393 MS.gene00168:CDS
CCAAACAGAAACAGAATTAA+TGG 0.226356 2.1:-58430149 None:intergenic
TTTCATTTCACTAAGTTTCT+TGG 0.235784 2.1:-58428478 None:intergenic
CTTGATATGATCCTTGTTCT+TGG 0.265549 2.1:-58428623 None:intergenic
TTGATTCTTAATCACCCTTT+TGG 0.265653 2.1:+58428969 MS.gene00168:CDS
GTTCTTGGTGATGATACTTT+AGG 0.270808 2.1:-58428608 None:intergenic
TAATGAACCACGCGCTTAAC+GGG 0.281787 2.1:-58429146 None:intergenic
CCATTAATTCTGTTTCTGTT+TGG 0.320002 2.1:+58430149 MS.gene00168:CDS
ATAATGAACCACGCGCTTAA+CGG 0.336606 2.1:-58429147 None:intergenic
TCGATTGAAGTGCTTGCATT+AGG 0.355406 2.1:-58429436 None:intergenic
ATCAAACCAAAATCATTAAC+TGG 0.365703 2.1:-58428843 None:intergenic
AAGAAGTTTGGTAGAATTGT+TGG 0.375164 2.1:-58428743 None:intergenic
ACCTCTTGAGGATCAAATAA+AGG 0.383647 2.1:-58429251 None:intergenic
AATGGTCATTGTCTTCTTGT+TGG 0.391390 2.1:+58430112 MS.gene00168:CDS
CAAACAGAAACAGAATTAAT+GGG 0.394231 2.1:-58430148 None:intergenic
GTTGATATATTGAAGAACTA+TGG 0.396369 2.1:+58428774 MS.gene00168:CDS
CAACCGGTGCTAAAGAAGTT+TGG 0.409389 2.1:-58428755 None:intergenic
TCTTGCACATTTGATATGAA+TGG 0.412387 2.1:+58430094 MS.gene00168:CDS
ATGGTCATTGTCTTCTTGTT+GGG 0.417701 2.1:+58430113 MS.gene00168:CDS
TTCTCTTAGATGTAATAAAT+CGG 0.421240 2.1:-58429612 None:intergenic
GAGTCTTTATCTTCAAAGAA+AGG 0.431602 2.1:+58429119 MS.gene00168:CDS
GATTCTTTGTTGAAGAGCTT+CGG 0.433882 2.1:-58429174 None:intergenic
GAAGATCTAGGAGACTATAA+TGG 0.434559 2.1:+58428522 MS.gene00168:CDS
CTCTCATGATTGTTTCAAGT+TGG 0.437396 2.1:-58429715 None:intergenic
TCTTCGTCGCAATCTGCATC+AGG 0.438613 2.1:+58428945 MS.gene00168:CDS
GTCTTTGTATCAAGCTGAAC+TGG 0.440740 2.1:+58430045 MS.gene00168:CDS
GACAAACAATATGCAAAAGA+TGG 0.445280 2.1:+58429226 MS.gene00168:CDS
AGGAATATTGTGGCTGAAGA+AGG 0.454210 2.1:+58429932 MS.gene00168:CDS
TGAAAGGTGATGAGAAGAAC+AGG 0.454352 2.1:+58429873 MS.gene00168:CDS
TGTCTTCTTGTTGGGTGGAA+AGG 0.455602 2.1:+58430121 MS.gene00168:CDS
CAAAGAATCGACAAAGAAAC+GGG 0.460522 2.1:+58429188 MS.gene00168:CDS
TAGTTCTTCAATATATCAAC+CGG 0.467500 2.1:-58428771 None:intergenic
CAAAATCATTAACTGGATGA+AGG 0.470878 2.1:-58428836 None:intergenic
GAAGATATCATGAAGATAGC+TGG 0.473521 2.1:+58428900 MS.gene00168:CDS
ACAAAGAATCGACAAAGAAA+CGG 0.477156 2.1:+58429187 MS.gene00168:CDS
AGGAAATCATCTTCTTGTGA+TGG 0.485850 2.1:-58428588 None:intergenic
AGATCCTTGGTTACCTCTTG+AGG 0.485987 2.1:-58429263 None:intergenic
AAGATAACGGCTATTGTGAC+TGG 0.492320 2.1:+58429485 MS.gene00168:CDS
TCCGCGACAACCATTACTAT+AGG 0.495008 2.1:-58429755 None:intergenic
ATTGATCTCGAAATCCGAAA+AGG 0.498196 2.1:+58429398 MS.gene00168:CDS
AAGGGCTGGATTTAGATCCT+TGG 0.500832 2.1:-58429276 None:intergenic
AGACACTGGTAAAAGATTGA+AGG 0.523064 2.1:+58429538 MS.gene00168:CDS
CCTTGAGCTTCTAAAGATAA+CGG 0.523094 2.1:+58429472 MS.gene00168:CDS
CTACCAAACTTCTTTAGCAC+CGG 0.524208 2.1:+58428752 MS.gene00168:CDS
AAAATCATTAACTGGATGAA+GGG 0.532257 2.1:-58428835 None:intergenic
GTCATTGTCTTCTTGTTGGG+TGG 0.532964 2.1:+58430116 MS.gene00168:CDS
CCGTTATCTTTAGAAGCTCA+AGG 0.533344 2.1:-58429472 None:intergenic
CGTTATCTTTAGAAGCTCAA+GGG 0.536414 2.1:-58429471 None:intergenic
TTGATCTCGAAATCCGAAAA+GGG 0.540863 2.1:+58429399 MS.gene00168:CDS
ATAATTGTATCGAATAAGAA+AGG 0.545181 2.1:-58429584 None:intergenic
AACTATAAATCCTATAGTAA+TGG 0.549134 2.1:+58429745 MS.gene00168:CDS
GTTGAGTTTCAATATAGTTG+AGG 0.552737 2.1:+58428419 MS.gene00168:CDS
ACAAACAATATGCAAAAGAT+GGG 0.554209 2.1:+58429227 MS.gene00168:CDS
AAGAGGTTACTACCTGATGA+AGG 0.555954 2.1:+58428807 MS.gene00168:CDS
TCCTATAGTAATGGTTGTCG+CGG 0.559207 2.1:+58429754 MS.gene00168:CDS
TTTCACAAGATTTGGAATGG+TGG 0.567481 2.1:+58430003 MS.gene00168:CDS
GACATGAATCTTCTTAGCAT+CGG 0.571430 2.1:-58429375 None:intergenic
ACCTTTATTTGATCCTCAAG+AGG 0.572203 2.1:+58429250 MS.gene00168:CDS
AATGAACCATGTCAAAGTGT+AGG 0.587676 2.1:-58428665 None:intergenic
AAGTATCATCACCAAGAACA+AGG 0.600969 2.1:+58428612 MS.gene00168:CDS
AAATCCGAAAAGGGTTACAA+TGG 0.612089 2.1:+58429408 MS.gene00168:CDS
TTAGCTTGTGCTGAGAAAGT+CGG 0.613798 2.1:+58429053 MS.gene00168:CDS
TGATAACCTACACTTTGACA+TGG 0.617264 2.1:+58428659 MS.gene00168:CDS
AGGTAACCCCGTTAAGCGCG+TGG 0.626614 2.1:+58429139 MS.gene00168:CDS
ACTATGGCAAATGCTTCAAG+AGG 0.626795 2.1:+58428790 MS.gene00168:CDS
AATGAACCACGCGCTTAACG+GGG 0.629117 2.1:-58429145 None:intergenic
GTTATCTTTAGAAGCTCAAG+GGG 0.631960 2.1:-58429470 None:intergenic
TCATGAAGATAGCTGGAACA+AGG 0.635820 2.1:+58428907 MS.gene00168:CDS
TCATGGAATTAGGAATATTG+TGG 0.639538 2.1:+58429922 MS.gene00168:CDS
ACTTGAAACAATCATGAGAG+TGG 0.655781 2.1:+58429718 MS.gene00168:CDS
GAAATGTGAAGATTGATGTG+TGG 0.658264 2.1:+58429972 MS.gene00168:CDS
TTTGTATCAAGCTGAACTGG+TGG 0.661886 2.1:+58430048 MS.gene00168:CDS
AAGCATATAGTTGAAGACAC+TGG 0.663403 2.1:+58429524 MS.gene00168:CDS
TGATCCTCAAGAGGTAACCA+AGG 0.673485 2.1:+58429259 MS.gene00168:CDS
GTGGCTAAAAGATTTCCTTG+TGG 0.684654 2.1:+58430067 MS.gene00168:CDS
GAAAGGTGATGAGAAGAACA+GGG 0.696660 2.1:+58429874 MS.gene00168:CDS
AGGTGATGAGAAGAACAGGG+TGG 0.718350 2.1:+58429877 MS.gene00168:CDS
AAATTGAGAGTAGACCGCAA+TGG 0.719135 2.1:-58429663 None:intergenic
AGATTCTGAGGAAACCATTG+CGG 0.723656 2.1:+58429649 MS.gene00168:CDS
AATGTGCAAGAATTTCCACA+AGG 0.746520 2.1:-58430082 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AACATTGAAATTTTACAAAA+TGG - chr2.1:58429323-58429342 None:intergenic 15.0%
!! AACTATAAATCCTATAGTAA+TGG + chr2.1:58429745-58429764 MS.gene00168:CDS 20.0%
!! ATAATTGTATCGAATAAGAA+AGG - chr2.1:58429587-58429606 None:intergenic 20.0%
!! TTCTCTTAGATGTAATAAAT+CGG - chr2.1:58429615-58429634 None:intergenic 20.0%
!!! GTTTTAGAATCAAAGTATTT+TGG + chr2.1:58429899-58429918 MS.gene00168:CDS 20.0%
!!! TTCATCCAATGATATTTTTT+GGG + chr2.1:58428452-58428471 MS.gene00168:CDS 20.0%
! AAAATCATTAACTGGATGAA+GGG - chr2.1:58428838-58428857 None:intergenic 25.0%
! ACAAACAATATGCAAAAGAT+GGG + chr2.1:58429227-58429246 MS.gene00168:CDS 25.0%
! ATCAAACCAAAATCATTAAC+TGG - chr2.1:58428846-58428865 None:intergenic 25.0%
! CAAACAGAAACAGAATTAAT+GGG - chr2.1:58430151-58430170 None:intergenic 25.0%
! GTTGATATATTGAAGAACTA+TGG + chr2.1:58428774-58428793 MS.gene00168:CDS 25.0%
! TAGTTCTTCAATATATCAAC+CGG - chr2.1:58428774-58428793 None:intergenic 25.0%
! TTTCATTTCACTAAGTTTCT+TGG - chr2.1:58428481-58428500 None:intergenic 25.0%
!! ATTTTTCACAAGATTTGGAA+TGG + chr2.1:58430000-58430019 MS.gene00168:CDS 25.0%
!!! GTTCATCCAATGATATTTTT+TGG + chr2.1:58428451-58428470 MS.gene00168:CDS 25.0%
AAGAAGTTTGGTAGAATTGT+TGG - chr2.1:58428746-58428765 None:intergenic 30.0%
ACAAAGAATCGACAAAGAAA+CGG + chr2.1:58429187-58429206 MS.gene00168:CDS 30.0%
CAAAATCATTAACTGGATGA+AGG - chr2.1:58428839-58428858 None:intergenic 30.0%
CAGAGAAACAAAAACCAAAA+GGG - chr2.1:58428986-58429005 None:intergenic 30.0%
CCAAACAGAAACAGAATTAA+TGG - chr2.1:58430152-58430171 None:intergenic 30.0%
GACAAACAATATGCAAAAGA+TGG + chr2.1:58429226-58429245 MS.gene00168:CDS 30.0%
GAGTCTTTATCTTCAAAGAA+AGG + chr2.1:58429119-58429138 MS.gene00168:CDS 30.0%
GGTTTCCCAAAAAATATCAT+TGG - chr2.1:58428460-58428479 None:intergenic 30.0%
GTTGAGTTTCAATATAGTTG+AGG + chr2.1:58428419-58428438 MS.gene00168:CDS 30.0%
TCATGGAATTAGGAATATTG+TGG + chr2.1:58429922-58429941 MS.gene00168:CDS 30.0%
TCTTGCACATTTGATATGAA+TGG + chr2.1:58430094-58430113 MS.gene00168:CDS 30.0%
! AAGTCTCTTTGCACTAATTT+TGG + chr2.1:58428549-58428568 MS.gene00168:CDS 30.0%
! ATGGAAAACTTGTGCAATTT+TGG + chr2.1:58428393-58428412 MS.gene00168:CDS 30.0%
! CTTCATCCAGTTAATGATTT+TGG + chr2.1:58428837-58428856 MS.gene00168:CDS 30.0%
! TTGATTCTTAATCACCCTTT+TGG + chr2.1:58428969-58428988 MS.gene00168:CDS 30.0%
! TTTTCTGTAGAAGAAGATCT+AGG + chr2.1:58428510-58428529 MS.gene00168:CDS 30.0%
!! AAAAAAGTGCTTCGATGAAA+CGG - chr2.1:58429817-58429836 None:intergenic 30.0%
!! AGAGCATTTTTCACAAGATT+TGG + chr2.1:58429995-58430014 MS.gene00168:CDS 30.0%
!! CCATTAATTCTGTTTCTGTT+TGG + chr2.1:58430149-58430168 MS.gene00168:CDS 30.0%
!! TCTTTTCAAGATAGATTCTG+AGG + chr2.1:58429637-58429656 MS.gene00168:CDS 30.0%
!! TGCATTTTTCGATTGTTTTG+AGG + chr2.1:58429844-58429863 MS.gene00168:CDS 30.0%
!!! AGTATTTTGGTCATGGAATT+AGG + chr2.1:58429912-58429931 MS.gene00168:CDS 30.0%
!!! GAATCAAAGTATTTTGGTCA+TGG + chr2.1:58429905-58429924 MS.gene00168:CDS 30.0%
!!! TGTTTTGAGGATTGTATGAA+AGG + chr2.1:58429857-58429876 MS.gene00168:CDS 30.0%
AAATCCGAAAAGGGTTACAA+TGG + chr2.1:58429408-58429427 MS.gene00168:CDS 35.0%
AAGCATATAGTTGAAGACAC+TGG + chr2.1:58429524-58429543 MS.gene00168:CDS 35.0%
AAGTATCATCACCAAGAACA+AGG + chr2.1:58428612-58428631 MS.gene00168:CDS 35.0%
AATGAACCATGTCAAAGTGT+AGG - chr2.1:58428668-58428687 None:intergenic 35.0%
AATGGTCATTGTCTTCTTGT+TGG + chr2.1:58430112-58430131 MS.gene00168:CDS 35.0%
AATGTGCAAGAATTTCCACA+AGG - chr2.1:58430085-58430104 None:intergenic 35.0%
ACCTCTTGAGGATCAAATAA+AGG - chr2.1:58429254-58429273 None:intergenic 35.0%
ACTTGAAACAATCATGAGAG+TGG + chr2.1:58429718-58429737 MS.gene00168:CDS 35.0%
AGACACTGGTAAAAGATTGA+AGG + chr2.1:58429538-58429557 MS.gene00168:CDS 35.0%
AGGAAATCATCTTCTTGTGA+TGG - chr2.1:58428591-58428610 None:intergenic 35.0%
ATCTTCAAAAAACGCGACAA+GGG - chr2.1:58429297-58429316 None:intergenic 35.0%
ATGGTCATTGTCTTCTTGTT+GGG + chr2.1:58430113-58430132 MS.gene00168:CDS 35.0%
ATTGATCTCGAAATCCGAAA+AGG + chr2.1:58429398-58429417 MS.gene00168:CDS 35.0%
CAAAGAATCGACAAAGAAAC+GGG + chr2.1:58429188-58429207 MS.gene00168:CDS 35.0%
CCAGAGAAACAAAAACCAAA+AGG - chr2.1:58428987-58429006 None:intergenic 35.0%
CTTGATATGATCCTTGTTCT+TGG - chr2.1:58428626-58428645 None:intergenic 35.0%
GAAATGTGAAGATTGATGTG+TGG + chr2.1:58429972-58429991 MS.gene00168:CDS 35.0%
GAAGATATCATGAAGATAGC+TGG + chr2.1:58428900-58428919 MS.gene00168:CDS 35.0%
GAAGATCTAGGAGACTATAA+TGG + chr2.1:58428522-58428541 MS.gene00168:CDS 35.0%
GACATGAATCTTCTTAGCAT+CGG - chr2.1:58429378-58429397 None:intergenic 35.0%
GATTCTTTGTTGAAGAGCTT+CGG - chr2.1:58429177-58429196 None:intergenic 35.0%
TCCACTTTGTGACACAAAAA+AGG + chr2.1:58428719-58428738 MS.gene00168:CDS 35.0%
TGATAACCTACACTTTGACA+TGG + chr2.1:58428659-58428678 MS.gene00168:CDS 35.0%
TTGATCTCGAAATCCGAAAA+GGG + chr2.1:58429399-58429418 MS.gene00168:CDS 35.0%
! ACCTTTATTTGATCCTCAAG+AGG + chr2.1:58429250-58429269 MS.gene00168:CDS 35.0%
! CCTTGAGCTTCTAAAGATAA+CGG + chr2.1:58429472-58429491 MS.gene00168:CDS 35.0%
! CGTTATCTTTAGAAGCTCAA+GGG - chr2.1:58429474-58429493 None:intergenic 35.0%
! CTCTCATGATTGTTTCAAGT+TGG - chr2.1:58429718-58429737 None:intergenic 35.0%
! GTTATCTTTAGAAGCTCAAG+GGG - chr2.1:58429473-58429492 None:intergenic 35.0%
! TTGTCCATTGTAACCCTTTT+CGG - chr2.1:58429415-58429434 None:intergenic 35.0%
! TTTCACAAGATTTGGAATGG+TGG + chr2.1:58430003-58430022 MS.gene00168:CDS 35.0%
! TTTTTGTGTCACAAAGTGGA+GGG - chr2.1:58428719-58428738 None:intergenic 35.0%
!! GTTCTTGGTGATGATACTTT+AGG - chr2.1:58428611-58428630 None:intergenic 35.0%
!!! CCTTTTGGTTTTTGTTTCTC+TGG + chr2.1:58428984-58429003 MS.gene00168:CDS 35.0%
!!! TCCTTTTTTGTGTCACAAAG+TGG - chr2.1:58428723-58428742 None:intergenic 35.0%
!!! TTTTTTGTGTCACAAAGTGG+AGG - chr2.1:58428720-58428739 None:intergenic 35.0%
AAATTGAGAGTAGACCGCAA+TGG - chr2.1:58429666-58429685 None:intergenic 40.0%
AAGAGGTTACTACCTGATGA+AGG + chr2.1:58428807-58428826 MS.gene00168:CDS 40.0%
ACTATGGCAAATGCTTCAAG+AGG + chr2.1:58428790-58428809 MS.gene00168:CDS 40.0%
AGGAATATTGTGGCTGAAGA+AGG + chr2.1:58429932-58429951 MS.gene00168:CDS 40.0%
ATAATGAACCACGCGCTTAA+CGG - chr2.1:58429150-58429169 None:intergenic 40.0%
CTACCAAACTTCTTTAGCAC+CGG + chr2.1:58428752-58428771 MS.gene00168:CDS 40.0%
GAAAGGTGATGAGAAGAACA+GGG + chr2.1:58429874-58429893 MS.gene00168:CDS 40.0%
GATCTTCAAAAAACGCGACA+AGG - chr2.1:58429298-58429317 None:intergenic 40.0%
GTCTTTGTATCAAGCTGAAC+TGG + chr2.1:58430045-58430064 MS.gene00168:CDS 40.0%
GTGGCTAAAAGATTTCCTTG+TGG + chr2.1:58430067-58430086 MS.gene00168:CDS 40.0%
TCATGAAGATAGCTGGAACA+AGG + chr2.1:58428907-58428926 MS.gene00168:CDS 40.0%
TCCTATAGTAATGGTTGTCG+CGG + chr2.1:58429754-58429773 MS.gene00168:CDS 40.0%
TGAAAGGTGATGAGAAGAAC+AGG + chr2.1:58429873-58429892 MS.gene00168:CDS 40.0%
TTTGTATCAAGCTGAACTGG+TGG + chr2.1:58430048-58430067 MS.gene00168:CDS 40.0%
! AAGATAACGGCTATTGTGAC+TGG + chr2.1:58429485-58429504 MS.gene00168:CDS 40.0%
! AGATTCTGAGGAAACCATTG+CGG + chr2.1:58429649-58429668 MS.gene00168:CDS 40.0%
! CCGTTATCTTTAGAAGCTCA+AGG - chr2.1:58429475-58429494 None:intergenic 40.0%
! TCGATTGAAGTGCTTGCATT+AGG - chr2.1:58429439-58429458 None:intergenic 40.0%
! TGAAGGGTTTTTCCTTCATC+AGG - chr2.1:58428822-58428841 None:intergenic 40.0%
!! TTAGCTTGTGCTGAGAAAGT+CGG + chr2.1:58429053-58429072 MS.gene00168:CDS 40.0%
AAGGGCTGGATTTAGATCCT+TGG - chr2.1:58429279-58429298 None:intergenic 45.0%
AGATCCTTGGTTACCTCTTG+AGG - chr2.1:58429266-58429285 None:intergenic 45.0%
GTCATTGTCTTCTTGTTGGG+TGG + chr2.1:58430116-58430135 MS.gene00168:CDS 45.0%
TAATGAACCACGCGCTTAAC+GGG - chr2.1:58429149-58429168 None:intergenic 45.0%
TCCGCGACAACCATTACTAT+AGG - chr2.1:58429758-58429777 None:intergenic 45.0%
TGATCCTCAAGAGGTAACCA+AGG + chr2.1:58429259-58429278 MS.gene00168:CDS 45.0%
TGTCTTCTTGTTGGGTGGAA+AGG + chr2.1:58430121-58430140 MS.gene00168:CDS 45.0%
! CAACCGGTGCTAAAGAAGTT+TGG - chr2.1:58428758-58428777 None:intergenic 45.0%
AATGAACCACGCGCTTAACG+GGG - chr2.1:58429148-58429167 None:intergenic 50.0%
AGGTGATGAGAAGAACAGGG+TGG + chr2.1:58429877-58429896 MS.gene00168:CDS 50.0%
TCAAAAAACGCGACAAGGGC+TGG - chr2.1:58429293-58429312 None:intergenic 50.0%
TCTTCGTCGCAATCTGCATC+AGG + chr2.1:58428945-58428964 MS.gene00168:CDS 50.0%
! CAAAGTGGAGGGTCTTTTGC+TGG - chr2.1:58428708-58428727 None:intergenic 50.0%
AGGTAACCCCGTTAAGCGCG+TGG + chr2.1:58429139-58429158 MS.gene00168:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 58428393 58430183 58428393 ID=MS.gene00168
chr2.1 mRNA 58428393 58430183 58428393 ID=MS.gene00168.t1;Parent=MS.gene00168
chr2.1 exon 58428393 58430183 58428393 ID=MS.gene00168.t1.exon1;Parent=MS.gene00168.t1
chr2.1 CDS 58428393 58430183 58428393 ID=cds.MS.gene00168.t1;Parent=MS.gene00168.t1
Gene Sequence

>MS.gene00168

ATGGAAAACTTGTGCAATTTTGGTGAGTTGAGTTTCAATATAGTTGAGGATAAACTTAGTTCATCCAATGATATTTTTTGGGAAACCAAGAAACTTAGTGAAATGAAAAATGTTCAATTTTCTGTAGAAGAAGATCTAGGAGACTATAATGGAACTAAGTCTCTTTGCACTAATTTTGGTTTCTTTCAAGATGATCCATCACAAGAAGATGATTTCCTAAAGTATCATCACCAAGAACAAGGATCATATCAAGATTATGAAACTTTTGATAACCTACACTTTGACATGGTTCATTTTGATGAACAACAATGTCCAGCAAAAGACCCTCCACTTTGTGACACAAAAAAGGACCAACAATTCTACCAAACTTCTTTAGCACCGGTTGATATATTGAAGAACTATGGCAAATGCTTCAAGAGGTTACTACCTGATGAAGGAAAAACCCTTCATCCAGTTAATGATTTTGGTTTGATCACAGATAATGAAAATGAGAGTAAGTTGTCAACTGAAGATATCATGAAGATAGCTGGAACAAGGTTCATACAATCATCATCTTCGTCGCAATCTGCATCAGGTTTGATTCTTAATCACCCTTTTGGTTTTTGTTTCTCTGGTTTATCTGATGAAGAGAAAGAAGATGTTTCACTTGTTGAATCACTCTTAGCTTGTGCTGAGAAAGTCGGATATCAACAATATGAACGAGCTAGAAAATTGTTATCGCAAATTGAGTCTTTATCTTCAAAGAAAGGTAACCCCGTTAAGCGCGTGGTTCATTATTTTGCCGAAGCTCTTCAACAAAGAATCGACAAAGAAACGGGAAGAGTTTCTGTTTCGACAAACAATATGCAAAAGATGGGACCTTTATTTGATCCTCAAGAGGTAACCAAGGATCTAAATCCAGCCCTTGTCGCGTTTTTTGAAGATCTTCCATTTTGTAAAATTTCAATGTTCACATGTGTACAAACTTTAATAGAAAATGTAACCGATGCTAAGAAGATTCATGTCATTGATCTCGAAATCCGAAAAGGGTTACAATGGACAATCCTAATGCAAGCACTTCAATCGAGAAACAAATGTCCCCTTGAGCTTCTAAAGATAACGGCTATTGTGACTGGAAACATAGACACTTCGAAGCATATAGTTGAAGACACTGGTAAAAGATTGAAGGACTTTGCGCGATATTTGAACATACCTTTCTTATTCGATACAATTATTGTATCCGATTTATTACATCTAAGAGAAGATCTTTTCAAGATAGATTCTGAGGAAACCATTGCGGTCTACTCTCAATTTGCACTTCAAAGCAAGATTCAACAATCAGACCAACTTGAAACAATCATGAGAGTGGTTAGAACTATAAATCCTATAGTAATGGTTGTCGCGGAGATTGAAGCTAATCACAATTCTAAATCTTTTGTGAACCGTTTCATCGAAGCACTTTTTTACTTTAGTGCATTTTTCGATTGTTTTGAGGATTGTATGAAAGGTGATGAGAAGAACAGGGTGGTTTTAGAATCAAAGTATTTTGGTCATGGAATTAGGAATATTGTGGCTGAAGAAGGAGCTGAAAGAAAGAGTAGAAATGTGAAGATTGATGTGTGGAGAGCATTTTTCACAAGATTTGGAATGGTGGAAACAGAATTGAGTATGATGTCTTTGTATCAAGCTGAACTGGTGGCTAAAAGATTTCCTTGTGGAAATTCTTGCACATTTGATATGAATGGTCATTGTCTTCTTGTTGGGTGGAAAGGAACACCCATTAATTCTGTTTCTGTTTGGAAATTCATTTGA
Protein sequence

>MS.gene00168.t1

MENLCNFGELSFNIVEDKLSSSNDIFWETKKLSEMKNVQFSVEEDLGDYNGTKSLCTNFGFFQDDPSQEDDFLKYHHQEQGSYQDYETFDNLHFDMVHFDEQQCPAKDPPLCDTKKDQQFYQTSLAPVDILKNYGKCFKRLLPDEGKTLHPVNDFGLITDNENESKLSTEDIMKIAGTRFIQSSSSSQSASGLILNHPFGFCFSGLSDEEKEDVSLVESLLACAEKVGYQQYERARKLLSQIESLSSKKGNPVKRVVHYFAEALQQRIDKETGRVSVSTNNMQKMGPLFDPQEVTKDLNPALVAFFEDLPFCKISMFTCVQTLIENVTDAKKIHVIDLEIRKGLQWTILMQALQSRNKCPLELLKITAIVTGNIDTSKHIVEDTGKRLKDFARYLNIPFLFDTIIVSDLLHLREDLFKIDSEETIAVYSQFALQSKIQQSDQLETIMRVVRTINPIVMVVAEIEANHNSKSFVNRFIEALFYFSAFFDCFEDCMKGDEKNRVVLESKYFGHGIRNIVAEEGAERKSRNVKIDVWRAFFTRFGMVETELSMMSLYQAELVAKRFPCGNSCTFDMNGHCLLVGWKGTPINSVSVWKFI