AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene001686

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001686.t1 MTR_1g093080 96.825 378 12 0 1 378 1 378 0.0 762
MS.gene001686.t1 MTR_1g093080 96.935 261 8 0 1 261 1 261 0.0 528
MS.gene001686.t1 MTR_1g093080 96.610 236 8 0 1 236 1 236 2.15e-171 478
MS.gene001686.t1 MTR_5g054300 57.368 380 126 14 1 374 10 359 2.28e-125 365
MS.gene001686.t1 MTR_4g113140 35.404 322 144 7 2 294 29 315 2.28e-48 169
MS.gene001686.t1 MTR_4g086835 34.930 355 146 11 10 309 44 368 9.97e-43 154
MS.gene001686.t1 MTR_4g086835 34.930 355 145 12 10 309 44 367 6.00e-41 149
MS.gene001686.t1 MTR_5g017980 33.333 330 143 12 2 324 35 294 1.38e-33 127
MS.gene001686.t1 MTR_5g017980 33.435 329 142 12 2 324 35 292 3.82e-33 126
MS.gene001686.t1 MTR_5g017980 33.636 330 141 13 2 324 35 293 6.53e-32 123
MS.gene001686.t1 MTR_7g115530 34.437 151 89 3 162 304 161 309 1.39e-15 77.8
MS.gene001686.t1 MTR_2g027860 56.061 66 29 0 211 276 49 114 1.63e-15 77.0
MS.gene001686.t1 MTR_2g034960 37.008 127 57 4 210 323 210 326 3.01e-15 77.8
MS.gene001686.t1 MTR_4g121020 53.521 71 32 1 210 280 110 179 9.50e-15 76.3
MS.gene001686.t1 MTR_2g450070 38.462 117 63 4 209 323 211 320 9.72e-15 76.3
MS.gene001686.t1 MTR_4g121020 53.521 71 32 1 210 280 214 283 1.00e-14 76.3
MS.gene001686.t1 MTR_4g121020 53.521 71 32 1 210 280 214 283 1.02e-14 76.3
MS.gene001686.t1 MTR_2g450070 38.462 117 63 4 209 323 211 320 1.06e-14 76.3
MS.gene001686.t1 MTR_7g088070 54.167 72 31 1 211 280 50 121 1.26e-14 73.9
MS.gene001686.t1 MTR_7g088070 55.385 65 29 0 211 275 50 114 1.63e-14 73.6
MS.gene001686.t1 MTR_4g131580 53.968 63 28 1 209 271 198 259 2.47e-14 74.7
MS.gene001686.t1 MTR_1g032570 48.611 72 36 1 200 271 120 190 3.30e-14 74.3
MS.gene001686.t1 MTR_1g032570 48.611 72 36 1 200 271 189 259 4.53e-14 73.9
MS.gene001686.t1 MTR_3g102600 43.038 79 43 2 215 292 87 164 5.43e-14 70.1
MS.gene001686.t1 MTR_3g106220 45.679 81 42 2 204 284 191 269 5.72e-14 73.9
MS.gene001686.t1 MTR_7g115530 37.815 119 66 2 162 272 161 279 7.37e-14 72.0
MS.gene001686.t1 MTR_7g117705 48.611 72 36 1 209 280 189 259 8.61e-14 72.8
MS.gene001686.t1 MTR_5g014040 51.515 66 31 1 204 269 304 368 1.02e-13 72.8
MS.gene001686.t1 MTR_7g115530 37.815 119 66 2 162 272 161 279 1.11e-13 71.6
MS.gene001686.t1 MTR_3g102600 43.038 79 43 2 215 292 136 213 1.33e-13 70.1
MS.gene001686.t1 MTR_5g041350 61.818 55 21 0 211 265 15 69 1.34e-13 71.6
MS.gene001686.t1 MTR_4g131570 50.000 66 32 1 206 271 198 262 1.38e-13 72.4
MS.gene001686.t1 MTR_7g115530 54.839 62 28 0 211 272 218 279 3.01e-13 71.2
MS.gene001686.t1 MTR_1g013170 52.381 63 29 1 209 271 189 250 3.24e-13 71.2
MS.gene001686.t1 MTR_4g098870 41.489 94 51 2 178 268 274 366 3.30e-13 71.2
MS.gene001686.t1 MTR_4g098870 41.489 94 51 2 178 268 274 366 3.38e-13 71.2
MS.gene001686.t1 MTR_7g098250 43.902 82 44 1 211 292 262 341 3.68e-13 70.9
MS.gene001686.t1 MTR_4g098870 41.489 94 51 2 178 268 313 405 3.79e-13 71.2
MS.gene001686.t1 MTR_4g098870 41.489 94 51 2 178 268 294 386 3.81e-13 71.2
MS.gene001686.t1 MTR_4g098870 41.489 94 51 2 178 268 294 386 4.01e-13 71.2
MS.gene001686.t1 MTR_2g027800 60.000 55 22 0 211 265 39 93 5.71e-13 69.3
MS.gene001686.t1 MTR_1g080330 50.794 63 31 0 211 273 175 237 6.07e-13 68.9
MS.gene001686.t1 MTR_2g027800 60.000 55 22 0 211 265 39 93 8.35e-13 68.9
MS.gene001686.t1 MTR_1g080330 50.794 63 31 0 211 273 175 237 1.01e-12 69.3
MS.gene001686.t1 MTR_4g064730 43.243 74 41 1 207 280 127 199 1.12e-12 68.6
MS.gene001686.t1 MTR_6g444980 60.000 55 22 0 211 265 35 89 1.50e-12 68.6
MS.gene001686.t1 MTR_6g032990 48.529 68 35 0 211 278 42 109 1.57e-12 68.6
MS.gene001686.t1 MTR_7g089010 40.244 82 45 2 188 269 71 148 1.65e-12 67.8
MS.gene001686.t1 MTR_6g444980 60.000 55 22 0 211 265 35 89 1.65e-12 68.6
MS.gene001686.t1 MTR_3g102590 43.590 78 41 2 209 284 141 217 1.71e-12 67.0
MS.gene001686.t1 MTR_2g086450 56.364 55 24 0 211 265 45 99 1.87e-12 68.6
MS.gene001686.t1 MTR_4g081710 58.182 55 23 0 211 265 17 71 1.88e-12 68.2
MS.gene001686.t1 MTR_1g053835 51.667 60 29 0 211 270 258 317 1.93e-12 68.6
MS.gene001686.t1 MTR_1g053830 49.057 53 27 0 213 265 20 72 2.15e-12 63.2
MS.gene001686.t1 MTR_4g078810 37.895 95 46 2 209 303 156 237 3.90e-12 67.8
MS.gene001686.t1 MTR_4g021790 48.571 70 35 1 200 269 157 225 4.45e-12 66.2
MS.gene001686.t1 MTR_1g090670 58.182 55 23 0 211 265 44 98 5.13e-12 66.2
MS.gene001686.t1 MTR_8g079940 46.479 71 37 1 210 280 208 277 7.32e-12 67.0
MS.gene001686.t1 MTR_2g090675 45.070 71 38 1 209 279 159 228 7.54e-12 66.6
MS.gene001686.t1 MTR_5g014040 47.887 71 31 2 204 269 264 333 8.08e-12 67.0
MS.gene001686.t1 MTR_5g014040 47.887 71 31 2 204 269 304 373 9.61e-12 66.6
MS.gene001686.t1 MTR_4g131600 46.970 66 33 2 204 268 193 257 9.65e-12 66.6
MS.gene001686.t1 MTR_2g023580 55.769 52 23 0 213 264 57 108 1.39e-11 64.3
MS.gene001686.t1 MTR_3g086100 40.698 86 45 2 200 280 112 196 2.02e-11 65.5
MS.gene001686.t1 MTR_7g093030 46.269 67 36 0 211 277 212 278 2.12e-11 64.7
MS.gene001686.t1 MTR_5g027440 50.820 61 28 1 211 269 25 85 2.14e-11 64.3
MS.gene001686.t1 MTR_7g115530 37.615 109 60 2 162 262 161 269 2.30e-11 64.3
MS.gene001686.t1 MTR_0450s0040 43.243 74 41 1 210 283 168 240 2.71e-11 64.7
MS.gene001686.t1 MTR_3g086100 40.698 86 45 2 200 280 183 267 3.64e-11 65.1
MS.gene001686.t1 MTR_7g115530 31.852 135 83 3 162 288 161 294 4.01e-11 63.5
MS.gene001686.t1 MTR_7g093030 46.269 67 36 0 211 277 212 278 5.30e-11 63.9
MS.gene001686.t1 MTR_7g026400 49.153 59 29 1 210 268 156 213 5.66e-11 64.3
MS.gene001686.t1 MTR_4g021760 40.506 79 46 1 208 286 166 243 8.96e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001686.t1 AT1G68670 47.929 338 126 12 10 326 16 324 1.40e-79 248
MS.gene001686.t1 AT1G25550 46.386 332 125 11 10 328 20 311 1.55e-77 243
MS.gene001686.t1 AT3G25790 43.869 367 166 8 10 372 20 350 5.80e-75 236
MS.gene001686.t1 AT1G13300 43.207 368 148 12 11 368 20 336 8.99e-71 225
MS.gene001686.t1 AT1G25550 49.231 260 104 8 10 261 20 259 1.00e-66 213
MS.gene001686.t1 AT3G25790 46.457 254 109 4 9 261 19 246 2.09e-56 185
MS.gene001686.t1 AT2G03500 35.802 324 126 9 8 294 36 314 1.98e-48 169
MS.gene001686.t1 AT4G37180 33.935 277 109 8 11 269 48 268 3.08e-33 127
MS.gene001686.t1 AT4G37180 33.574 277 117 8 11 269 48 275 1.09e-30 120
MS.gene001686.t1 AT1G49560 32.542 295 132 9 10 293 30 268 5.15e-30 118
MS.gene001686.t1 AT4G37180 33.086 269 106 8 11 261 48 260 1.49e-28 112
MS.gene001686.t1 AT1G49560 31.939 263 118 8 10 261 30 242 4.14e-23 97.8
MS.gene001686.t1 AT2G01060 57.812 64 27 0 211 274 15 78 2.17e-15 76.3
MS.gene001686.t1 AT3G16857 35.252 139 79 3 199 336 155 283 8.27e-15 76.3
MS.gene001686.t1 AT3G16857 34.752 141 81 3 199 338 225 355 8.83e-15 76.3
MS.gene001686.t1 AT3G16857 34.752 141 81 3 199 338 225 355 1.03e-14 76.3
MS.gene001686.t1 AT5G58080 50.000 66 32 1 204 269 187 251 4.26e-14 74.3
MS.gene001686.t1 AT3G04030 53.846 65 30 0 211 275 45 109 9.30e-14 72.4
MS.gene001686.t1 AT3G04030 53.846 65 30 0 211 275 45 109 1.09e-13 72.4
MS.gene001686.t1 AT2G25180 31.902 163 94 5 209 356 193 353 1.24e-13 72.8
MS.gene001686.t1 AT5G05090 45.570 79 41 2 192 269 61 138 1.88e-13 70.5
MS.gene001686.t1 AT5G06800 53.226 62 29 0 211 272 192 253 2.96e-13 70.1
MS.gene001686.t1 AT5G18240 58.182 55 23 0 211 265 45 99 3.79e-13 70.9
MS.gene001686.t1 AT5G18240 58.182 55 23 0 211 265 45 99 3.79e-13 70.9
MS.gene001686.t1 AT5G18240 58.182 55 23 0 211 265 45 99 4.17e-13 70.5
MS.gene001686.t1 AT5G18240 58.182 55 23 0 211 265 45 99 4.17e-13 70.5
MS.gene001686.t1 AT5G18240 58.182 55 23 0 211 265 45 99 4.33e-13 70.5
MS.gene001686.t1 AT2G01760 44.156 77 42 1 210 286 134 209 4.90e-13 69.7
MS.gene001686.t1 AT5G44190 43.750 80 44 1 200 279 136 214 6.23e-13 69.7
MS.gene001686.t1 AT2G01760 44.156 77 42 1 210 286 227 302 6.82e-13 70.1
MS.gene001686.t1 AT5G44190 43.750 80 44 1 200 279 136 214 7.12e-13 69.7
MS.gene001686.t1 AT2G01760 44.156 77 42 1 210 286 199 274 7.67e-13 69.7
MS.gene001686.t1 AT5G06800 53.226 62 29 0 211 272 192 253 1.01e-12 69.3
MS.gene001686.t1 AT3G10760 44.444 81 43 2 190 269 83 162 1.06e-12 68.9
MS.gene001686.t1 AT5G06800 53.226 62 29 0 211 272 192 253 1.06e-12 69.3
MS.gene001686.t1 AT2G40970 45.070 71 38 1 199 269 93 162 1.12e-12 67.8
MS.gene001686.t1 AT3G46640 33.113 151 92 4 134 276 59 208 1.31e-12 68.6
MS.gene001686.t1 AT3G46640 46.269 67 35 1 210 276 143 208 1.54e-12 68.2
MS.gene001686.t1 AT3G46640 46.269 67 35 1 210 276 143 208 1.54e-12 68.2
MS.gene001686.t1 AT5G45580 44.595 74 41 0 211 284 23 96 1.60e-12 67.8
MS.gene001686.t1 AT5G45580 44.595 74 41 0 211 284 7 80 1.61e-12 67.4
MS.gene001686.t1 AT1G79430 60.000 55 22 0 211 265 34 88 1.64e-12 68.6
MS.gene001686.t1 AT3G04450 51.667 60 29 0 211 270 238 297 1.72e-12 68.2
MS.gene001686.t1 AT3G24120 58.182 55 23 0 211 265 41 95 2.40e-12 67.4
MS.gene001686.t1 AT5G59570 38.318 107 64 2 163 269 94 198 2.48e-12 67.4
MS.gene001686.t1 AT5G59570 38.318 107 64 2 163 269 94 198 2.48e-12 67.4
MS.gene001686.t1 AT5G29000 49.206 63 32 0 211 273 231 293 2.57e-12 67.4
MS.gene001686.t1 AT3G24120 58.182 55 23 0 211 265 41 95 2.80e-12 67.4
MS.gene001686.t1 AT4G13640 58.182 55 23 0 211 265 37 91 2.86e-12 67.4
MS.gene001686.t1 AT4G13640 58.182 55 23 0 211 265 37 91 2.97e-12 67.0
MS.gene001686.t1 AT3G13040 52.459 61 29 0 210 270 240 300 3.13e-12 68.2
MS.gene001686.t1 AT3G13040 52.459 61 29 0 210 270 240 300 3.13e-12 68.2
MS.gene001686.t1 AT3G13040 52.459 61 29 0 210 270 240 300 3.13e-12 68.2
MS.gene001686.t1 AT3G04450 51.667 60 29 0 211 270 238 297 3.40e-12 67.8
MS.gene001686.t1 AT3G04450 51.667 60 29 0 211 270 198 257 4.03e-12 67.4
MS.gene001686.t1 AT3G04450 51.667 60 29 0 211 270 198 257 4.03e-12 67.4
MS.gene001686.t1 AT2G20570 47.945 73 37 1 207 279 149 220 4.27e-12 67.4
MS.gene001686.t1 AT5G29000 49.206 63 32 0 211 273 231 293 6.16e-12 67.0
MS.gene001686.t1 AT3G04030 52.308 65 30 1 211 275 45 108 6.36e-12 67.0
MS.gene001686.t1 AT4G28610 51.667 60 29 0 211 270 225 284 6.84e-12 67.0
MS.gene001686.t1 AT5G29000 49.206 63 32 0 211 273 188 250 6.95e-12 66.6
MS.gene001686.t1 AT5G29000 49.206 63 32 0 211 273 188 250 6.95e-12 66.6
MS.gene001686.t1 AT3G12730 56.364 55 24 0 211 265 23 77 8.62e-12 65.1
MS.gene001686.t1 AT2G42660 52.632 57 27 0 216 272 55 111 1.16e-11 64.7
MS.gene001686.t1 AT2G38300 51.923 52 25 0 213 264 56 107 1.94e-11 65.1
MS.gene001686.t1 AT4G31920 42.105 76 43 1 204 279 176 250 2.34e-11 65.5
MS.gene001686.t1 AT2G06020 50.909 55 27 0 211 265 86 140 2.85e-11 64.3
MS.gene001686.t1 AT1G49190 36.486 74 44 1 196 266 200 273 5.92e-11 63.9
MS.gene001686.t1 AT4G04605 51.923 52 25 0 213 264 16 67 7.92e-11 62.0

Find 78 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTAACTCTATCTTCTTTA+TGG 0.063110 1.3:+64738147 None:intergenic
CACAATGCAGCGTTTCAAAA+TGG 0.179894 1.3:-64738887 None:intergenic
ACAAAAGTGGTTGCACCTTC+TGG 0.247597 1.3:-64737331 MS.gene001686:CDS
CATATCTACAAACCTGTAAA+TGG 0.279375 1.3:+64737738 None:intergenic
TGAAGTCAAAAGCCATTTAC+AGG 0.284068 1.3:-64737750 MS.gene001686:intron
AACGAAAGAGGAAGTTCTTT+AGG 0.299727 1.3:+64738808 None:intergenic
GATGTCTCCAACATTGTTAC+TGG 0.321828 1.3:+64738043 None:intergenic
ATTACCTCTCTGATTTGCTT+TGG 0.356836 1.3:+64737797 None:intergenic
GTCGTTCTTGTTCTAATGCT+TGG 0.361767 1.3:+64738848 None:intergenic
CTTTCGTTATTTGTTGAACT+TGG 0.382926 1.3:+64737457 None:intergenic
GGCTTAGATCTGTTCAGTTA+TGG 0.389861 1.3:-64738406 MS.gene001686:CDS
CCCCACCATACTCTTGTTGT+TGG 0.395076 1.3:+64737383 None:intergenic
GGAATAAACTCTTCTAAAAC+AGG 0.409660 1.3:+64738548 None:intergenic
TTTGTCCAACAACAAGAGTA+TGG 0.414336 1.3:-64737388 MS.gene001686:CDS
ATTATTACCTCTTCAGATGA+AGG 0.417768 1.3:+64738374 None:intergenic
ACAATGCAGCGTTTCAAAAT+GGG 0.423030 1.3:-64738886 MS.gene001686:CDS
GTAGCTTCATCAACAACAAC+AGG 0.429695 1.3:-64737361 MS.gene001686:CDS
TGCTCAAACCTCTTGAACTC+TGG 0.432408 1.3:+64737259 None:intergenic
GCAGCGACGGTGCTGGTCGC+AGG 0.434166 1.3:-64737991 MS.gene001686:CDS
GTTTATTCCAATTAAGAAAA+GGG 0.437351 1.3:-64738534 MS.gene001686:CDS
GTTGCACAAGCGATTCTTGA+AGG 0.440339 1.3:-64737967 MS.gene001686:CDS
AGTTTATTCCAATTAAGAAA+AGG 0.446516 1.3:-64738535 MS.gene001686:CDS
ACTCTATCTTCTTTATGGAA+TGG 0.449526 1.3:+64738152 None:intergenic
GCTTGTCGACAACAATTGTC+TGG 0.459321 1.3:-64738638 MS.gene001686:CDS
CATCAAAAGAGACGAGAAAG+AGG 0.461312 1.3:-64738024 MS.gene001686:CDS
TAAAGAACTTCCTCTTTCGT+TGG 0.464319 1.3:-64738806 MS.gene001686:CDS
TTGAAGGCGCTTCAGCAACT+TGG 0.469776 1.3:-64737951 MS.gene001686:CDS
AGAGAGGTAATGAATGTTGA+TGG 0.478733 1.3:-64737785 MS.gene001686:CDS
GCATTCAATCTCAGATGAAA+GGG 0.480171 1.3:-64737239 MS.gene001686:CDS
AGGTGCTGCTGTTTGTGAAT+TGG 0.480961 1.3:+64737432 None:intergenic
CTCTCTGATTTGCTTTGGTG+TGG 0.488332 1.3:+64737802 None:intergenic
GTGTTGGAATTGAAGAGAAA+TGG 0.488831 1.3:-64738197 MS.gene001686:CDS
ACAACTGAGTATAATTTGAA+TGG 0.489356 1.3:-64738611 MS.gene001686:CDS
TCAATAACCAGGGGAAGTAT+TGG 0.491273 1.3:+64737150 None:intergenic
TTGGTCTTCTAGTATGCAAC+CGG 0.498211 1.3:+64737476 None:intergenic
ACAAGTGAATTGTTAAGTAA+AGG 0.498649 1.3:-64738119 MS.gene001686:CDS
ATGTTGCCTACAAGGACAAA+AGG 0.503411 1.3:+64737412 None:intergenic
AAGGCGCTTCAGCAACTTGG+TGG 0.504237 1.3:-64737948 MS.gene001686:CDS
ACTTGAGGATGTGTGGCTAC+TGG 0.518302 1.3:+64737298 None:intergenic
TTCTTAATTGTAGCAACTTG+AGG 0.518381 1.3:+64737283 None:intergenic
AGCATTCAATCTCAGATGAA+AGG 0.522044 1.3:-64737240 MS.gene001686:CDS
AAGAGAAATGGAAGTGGTGG+TGG 0.523432 1.3:-64738185 MS.gene001686:CDS
TGTGTAGAGGATGAAGATGT+AGG 0.529070 1.3:+64737181 None:intergenic
TTAAGAAACCAGAGTTCAAG+AGG 0.534779 1.3:-64737267 MS.gene001686:CDS
TCCAACAACAAGAGTATGGT+GGG 0.538170 1.3:-64737384 MS.gene001686:CDS
AGAGAAAGCAGCGACGGTGC+TGG 0.540249 1.3:-64737998 MS.gene001686:CDS
AGAACTCACGGCAGCTACAC+CGG 0.545612 1.3:+64738082 None:intergenic
GTAACGAGTTCCAACGAAAG+AGG 0.570810 1.3:+64738796 None:intergenic
GGCAGCTACACCGGTCGAAG+AGG 0.574491 1.3:+64738091 None:intergenic
AAAATTAAAACTCAATAACC+AGG 0.575545 1.3:+64737139 None:intergenic
ATAATAAGAAGAAATCTGAC+TGG 0.576819 1.3:-64738427 MS.gene001686:CDS
AGGAAAAGAGAAAGCAGCGA+CGG 0.577313 1.3:-64738004 MS.gene001686:CDS
ACTACTACCAATACTTCCCC+TGG 0.577357 1.3:-64737157 MS.gene001686:CDS
TAAGAAAACAGTGCCTGTGT+TGG 0.581215 1.3:-64738213 MS.gene001686:CDS
TTCAGATCCTTCATCTGAAG+AGG 0.584122 1.3:-64738381 MS.gene001686:intron
AAAGGACAACCCTCTTCGAC+CGG 0.587220 1.3:-64738101 MS.gene001686:CDS
AAATTAAAACTCAATAACCA+GGG 0.590216 1.3:+64737140 None:intergenic
GCAGCTACACCGGTCGAAGA+GGG 0.597437 1.3:+64738092 None:intergenic
TTTCTCTTCAATTCCAACAC+AGG 0.600323 1.3:+64738200 None:intergenic
AACAGGGGAAATGACAAAAG+TGG 0.604173 1.3:-64737344 MS.gene001686:CDS
ATTGGTAGTAGTATGTGTAG+AGG 0.610237 1.3:+64737168 None:intergenic
AATGTCACCAGTAACAATGT+TGG 0.610482 1.3:-64738050 MS.gene001686:CDS
GTCCAACAACAAGAGTATGG+TGG 0.618792 1.3:-64737385 MS.gene001686:CDS
GAACAGACAACATCAACAGA+TGG 0.623810 1.3:-64738572 MS.gene001686:CDS
ATTAGCCGCATTAGAACTCA+CGG 0.629576 1.3:+64738070 None:intergenic
GAAAGGGGTAATAACAGTGA+AGG 0.640994 1.3:-64737223 MS.gene001686:CDS
ACTGGTGCATATATTCCAGA+AGG 0.644016 1.3:+64737316 None:intergenic
TTGAAGAGAAATGGAAGTGG+TGG 0.651388 1.3:-64738188 MS.gene001686:CDS
TCGTTGGAACTCGTTACACA+AGG 0.654033 1.3:-64738790 MS.gene001686:intron
CATTCAATCTCAGATGAAAG+GGG 0.655574 1.3:-64737238 MS.gene001686:CDS
AGCTGCCGTGAGTTCTAATG+CGG 0.671481 1.3:-64738075 MS.gene001686:CDS
TGTAGCAACTTGAGGATGTG+TGG 0.683531 1.3:+64737291 None:intergenic
GAATTGAAGAGAAATGGAAG+TGG 0.689165 1.3:-64738191 MS.gene001686:CDS
TAGCTTCATCAACAACAACA+GGG 0.693117 1.3:-64737360 MS.gene001686:CDS
AATTAAAACTCAATAACCAG+GGG 0.700665 1.3:+64737141 None:intergenic
CCAACAACAAGAGTATGGTG+GGG 0.702776 1.3:-64737383 MS.gene001686:CDS
AGCTTCATCAACAACAACAG+GGG 0.709783 1.3:-64737359 MS.gene001686:CDS
CACACCAAAGCAAATCAGAG+AGG 0.720773 1.3:-64737801 MS.gene001686:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAATTGATTTTAATTG+AGG + chr1.3:64738513-64738532 None:intergenic 10.0%
!!! AAAATAATTTTGGATGTTTT+TGG - chr1.3:64737353-64737372 MS.gene001686:CDS 15.0%
!!! AAGTTTGTATTTTTTTTATC+TGG - chr1.3:64737313-64737332 MS.gene001686:CDS 15.0%
!!! AGTTTGTATTTTTTTTATCT+GGG - chr1.3:64737314-64737333 MS.gene001686:CDS 15.0%
!! AGTTTATTCCAATTAAGAAA+AGG - chr1.3:64737498-64737517 MS.gene001686:intron 20.0%
!! GTTTATTCCAATTAAGAAAA+GGG - chr1.3:64737499-64737518 MS.gene001686:intron 20.0%
!!! GTTTTTGTGAAAAATTGTTT+AGG - chr1.3:64737790-64737809 MS.gene001686:CDS 20.0%
!!! TTACTTTTTGTTAAACTGAA+GGG - chr1.3:64737731-64737750 MS.gene001686:intron 20.0%
!!! TTTACTTTTTGTTAAACTGA+AGG - chr1.3:64737730-64737749 MS.gene001686:intron 20.0%
!!! TTTTTCACAAAAACAAAACA+AGG + chr1.3:64737784-64737803 None:intergenic 20.0%
! AAAATTGACAACAAAAGAGT+TGG + chr1.3:64738330-64738349 None:intergenic 25.0%
! AAATTGACAACAAAAGAGTT+GGG + chr1.3:64738329-64738348 None:intergenic 25.0%
! ACAACTGAGTATAATTTGAA+TGG - chr1.3:64737422-64737441 MS.gene001686:CDS 25.0%
! ACAAGTGAATTGTTAAGTAA+AGG - chr1.3:64737914-64737933 MS.gene001686:intron 25.0%
! ATAATAAGAAGAAATCTGAC+TGG - chr1.3:64737606-64737625 MS.gene001686:intron 25.0%
! TGTTAACTCTATCTTCTTTA+TGG + chr1.3:64737889-64737908 None:intergenic 25.0%
ACTCTATCTTCTTTATGGAA+TGG + chr1.3:64737884-64737903 None:intergenic 30.0%
ATTATTACCTCTTCAGATGA+AGG + chr1.3:64737662-64737681 None:intergenic 30.0%
CATATCTACAAACCTGTAAA+TGG + chr1.3:64738298-64738317 None:intergenic 30.0%
GAAATCCTATGCAATTGAAA+GGG + chr1.3:64738434-64738453 None:intergenic 30.0%
TGAAATCCTATGCAATTGAA+AGG + chr1.3:64738435-64738454 None:intergenic 30.0%
TTCTTAATTGTAGCAACTTG+AGG + chr1.3:64738753-64738772 None:intergenic 30.0%
! CTTTCGTTATTTGTTGAACT+TGG + chr1.3:64738579-64738598 None:intergenic 30.0%
!! GGAATAAACTCTTCTAAAAC+AGG + chr1.3:64737488-64737507 None:intergenic 30.0%
!!! GTTTTTTGCTTCTGTTCTAT+AGG - chr1.3:64738206-64738225 MS.gene001686:CDS 30.0%
!!! TTTTGACTTCATCATTTGTG+AGG + chr1.3:64738274-64738293 None:intergenic 30.0%
AACGAAAGAGGAAGTTCTTT+AGG + chr1.3:64737228-64737247 None:intergenic 35.0%
AATGTCACCAGTAACAATGT+TGG - chr1.3:64737983-64738002 MS.gene001686:CDS 35.0%
AGAGAGGTAATGAATGTTGA+TGG - chr1.3:64738248-64738267 MS.gene001686:intron 35.0%
AGCATTCAATCTCAGATGAA+AGG - chr1.3:64738793-64738812 MS.gene001686:CDS 35.0%
CATTCAATCTCAGATGAAAG+GGG - chr1.3:64738795-64738814 MS.gene001686:CDS 35.0%
GAATTGAAGAGAAATGGAAG+TGG - chr1.3:64737842-64737861 MS.gene001686:intron 35.0%
GCATTCAATCTCAGATGAAA+GGG - chr1.3:64738794-64738813 MS.gene001686:CDS 35.0%
GTGTTGGAATTGAAGAGAAA+TGG - chr1.3:64737836-64737855 MS.gene001686:intron 35.0%
TAAAGAACTTCCTCTTTCGT+TGG - chr1.3:64737227-64737246 MS.gene001686:CDS 35.0%
TAGCTTCATCAACAACAACA+GGG - chr1.3:64738673-64738692 MS.gene001686:intron 35.0%
TGAAGTCAAAAGCCATTTAC+AGG - chr1.3:64738283-64738302 MS.gene001686:intron 35.0%
TTAAGAAACCAGAGTTCAAG+AGG - chr1.3:64738766-64738785 MS.gene001686:intron 35.0%
TTAGTCCCTTTCAATTGCAT+AGG - chr1.3:64738426-64738445 MS.gene001686:CDS 35.0%
TTTCTCTTCAATTCCAACAC+AGG + chr1.3:64737836-64737855 None:intergenic 35.0%
TTTGTCCAACAACAAGAGTA+TGG - chr1.3:64738645-64738664 MS.gene001686:CDS 35.0%
! ATTGGTAGTAGTATGTGTAG+AGG + chr1.3:64738868-64738887 None:intergenic 35.0%
! GAAGAAGCCCTTTTCTTAAT+TGG + chr1.3:64737509-64737528 None:intergenic 35.0%
! TCTGTTTTGCACAGAAATAC+CGG - chr1.3:64738538-64738557 MS.gene001686:CDS 35.0%
!! ATTACCTCTCTGATTTGCTT+TGG + chr1.3:64738239-64738258 None:intergenic 35.0%
AACAGGGGAAATGACAAAAG+TGG - chr1.3:64738689-64738708 MS.gene001686:intron 40.0%
ACTGGTGCATATATTCCAGA+AGG + chr1.3:64738720-64738739 None:intergenic 40.0%
AGCTTCATCAACAACAACAG+GGG - chr1.3:64738674-64738693 MS.gene001686:intron 40.0%
ATGTTGCCTACAAGGACAAA+AGG + chr1.3:64738624-64738643 None:intergenic 40.0%
ATTAGCCGCATTAGAACTCA+CGG + chr1.3:64737966-64737985 None:intergenic 40.0%
CATCAAAAGAGACGAGAAAG+AGG - chr1.3:64738009-64738028 MS.gene001686:CDS 40.0%
GAAAGGGGTAATAACAGTGA+AGG - chr1.3:64738810-64738829 MS.gene001686:CDS 40.0%
GAACAGACAACATCAACAGA+TGG - chr1.3:64737461-64737480 MS.gene001686:CDS 40.0%
GATGTCTCCAACATTGTTAC+TGG + chr1.3:64737993-64738012 None:intergenic 40.0%
GGACAAAAATGTTGCCTACA+AGG + chr1.3:64738632-64738651 None:intergenic 40.0%
GGCTTAGATCTGTTCAGTTA+TGG - chr1.3:64737627-64737646 MS.gene001686:intron 40.0%
GTAGCTTCATCAACAACAAC+AGG - chr1.3:64738672-64738691 MS.gene001686:intron 40.0%
GTCGTTCTTGTTCTAATGCT+TGG + chr1.3:64737188-64737207 None:intergenic 40.0%
TAAGAAAACAGTGCCTGTGT+TGG - chr1.3:64737820-64737839 MS.gene001686:intron 40.0%
TGTGTAGAGGATGAAGATGT+AGG + chr1.3:64738855-64738874 None:intergenic 40.0%
TTCAGATCCTTCATCTGAAG+AGG - chr1.3:64737652-64737671 MS.gene001686:intron 40.0%
TTGAAGAGAAATGGAAGTGG+TGG - chr1.3:64737845-64737864 MS.gene001686:intron 40.0%
! TCAATAACCAGGGGAAGTAT+TGG + chr1.3:64738886-64738905 None:intergenic 40.0%
! TCCAACAACAAGAGTATGGT+GGG - chr1.3:64738649-64738668 MS.gene001686:CDS 40.0%
! TTGGTCTTCTAGTATGCAAC+CGG + chr1.3:64738560-64738579 None:intergenic 40.0%
AAGAGAAATGGAAGTGGTGG+TGG - chr1.3:64737848-64737867 MS.gene001686:intron 45.0%
ACAAAAGTGGTTGCACCTTC+TGG - chr1.3:64738702-64738721 MS.gene001686:intron 45.0%
ACTACTACCAATACTTCCCC+TGG - chr1.3:64738876-64738895 MS.gene001686:CDS 45.0%
AGGAAAAGAGAAAGCAGCGA+CGG - chr1.3:64738029-64738048 MS.gene001686:CDS 45.0%
CACACCAAAGCAAATCAGAG+AGG - chr1.3:64738232-64738251 MS.gene001686:intron 45.0%
GCTTGTCGACAACAATTGTC+TGG - chr1.3:64737395-64737414 MS.gene001686:CDS 45.0%
GTAACGAGTTCCAACGAAAG+AGG + chr1.3:64737240-64737259 None:intergenic 45.0%
GTTGCACAAGCGATTCTTGA+AGG - chr1.3:64738066-64738085 MS.gene001686:CDS 45.0%
TCGTTGGAACTCGTTACACA+AGG - chr1.3:64737243-64737262 MS.gene001686:CDS 45.0%
TGCTCAAACCTCTTGAACTC+TGG + chr1.3:64738777-64738796 None:intergenic 45.0%
TGTAGCAACTTGAGGATGTG+TGG + chr1.3:64738745-64738764 None:intergenic 45.0%
! CCAACAACAAGAGTATGGTG+GGG - chr1.3:64738650-64738669 MS.gene001686:intron 45.0%
! GTCCAACAACAAGAGTATGG+TGG - chr1.3:64738648-64738667 MS.gene001686:CDS 45.0%
!! AGGTGCTGCTGTTTGTGAAT+TGG + chr1.3:64738604-64738623 None:intergenic 45.0%
!! CTCTCTGATTTGCTTTGGTG+TGG + chr1.3:64738234-64738253 None:intergenic 45.0%
!! ATATAAAAAAAAAACAAAAA+GGG + chr1.3:64737279-64737298 None:intergenic 5.0%
!! TATATAAAAAAAAAACAAAA+AGG + chr1.3:64737280-64737299 None:intergenic 5.0%
!!! TTATTTTTGAAAAATAATTT+TGG - chr1.3:64737343-64737362 MS.gene001686:CDS 5.0%
AAAGGACAACCCTCTTCGAC+CGG - chr1.3:64737932-64737951 MS.gene001686:intron 50.0%
ACTTGAGGATGTGTGGCTAC+TGG + chr1.3:64738738-64738757 None:intergenic 50.0%
! AGCTGCCGTGAGTTCTAATG+CGG - chr1.3:64737958-64737977 MS.gene001686:CDS 50.0%
! CCCCACCATACTCTTGTTGT+TGG + chr1.3:64738653-64738672 None:intergenic 50.0%
! GCAGCACCTTTTGTCCTTGT+AGG - chr1.3:64738615-64738634 MS.gene001686:CDS 50.0%
!! TTGAAGGCGCTTCAGCAACT+TGG - chr1.3:64738082-64738101 MS.gene001686:CDS 50.0%
AGAACTCACGGCAGCTACAC+CGG + chr1.3:64737954-64737973 None:intergenic 55.0%
! AAGGCGCTTCAGCAACTTGG+TGG - chr1.3:64738085-64738104 MS.gene001686:CDS 55.0%
AGAGAAAGCAGCGACGGTGC+TGG - chr1.3:64738035-64738054 MS.gene001686:CDS 60.0%
GCAGCTACACCGGTCGAAGA+GGG + chr1.3:64737944-64737963 None:intergenic 60.0%
GGCAGCTACACCGGTCGAAG+AGG + chr1.3:64737945-64737964 None:intergenic 65.0%
!! GCAGCGACGGTGCTGGTCGC+AGG - chr1.3:64738042-64738061 MS.gene001686:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 64737150 64738905 64737150 ID=MS.gene001686
chr1.3 mRNA 64737150 64738905 64737150 ID=MS.gene001686.t1;Parent=MS.gene001686
chr1.3 exon 64738791 64738905 64738791 ID=MS.gene001686.t1.exon1;Parent=MS.gene001686.t1
chr1.3 CDS 64738791 64738905 64738791 ID=cds.MS.gene001686.t1;Parent=MS.gene001686.t1
chr1.3 exon 64738382 64738668 64738382 ID=MS.gene001686.t1.exon2;Parent=MS.gene001686.t1
chr1.3 CDS 64738382 64738668 64738382 ID=cds.MS.gene001686.t1;Parent=MS.gene001686.t1
chr1.3 exon 64737940 64738243 64737940 ID=MS.gene001686.t1.exon3;Parent=MS.gene001686.t1
chr1.3 CDS 64737940 64738243 64737940 ID=cds.MS.gene001686.t1;Parent=MS.gene001686.t1
chr1.3 exon 64737751 64737827 64737751 ID=MS.gene001686.t1.exon4;Parent=MS.gene001686.t1
chr1.3 CDS 64737751 64737827 64737751 ID=cds.MS.gene001686.t1;Parent=MS.gene001686.t1
chr1.3 exon 64737150 64737503 64737150 ID=MS.gene001686.t1.exon5;Parent=MS.gene001686.t1
chr1.3 CDS 64737150 64737503 64737150 ID=cds.MS.gene001686.t1;Parent=MS.gene001686.t1
Gene Sequence

>MS.gene001686

ATGCAGCGTTTCAAAATGGGTTTCTCTGATTATATCCAAGCATTAGAACAAGAACGACGTAAGATTCAAGTCTTTCCTAAAGAACTTCCTCTTTCGTTGGAACTCGTTACACAAGGTACGTACATACCCTTTTTGTTTTTTTTTTATATATGATTATGAGTTAAAGTTTGTATTTTTTTTATCTGGGTTTGTTTTATTTTTGAAAAATAATTTTGGATGTTTTTGGTTGATTATTAGCTATTGAAGCTTGTCGACAACAATTGTCTGGAACAACAACTGAGTATAATTTGAATGGACAATCTGAGTGTTCTGAACAGACAACATCAACAGATGGACCTGTTTTAGAAGAGTTTATTCCAATTAAGAAAAGGGCTTCTTCTTATTCTCATGAAGTGTTTGATGATGTAGAAGATTATGATCAACATTTTCATCACAAGCAACAAAAACTTTCATTAGATAATAAGAAGAAATCTGACTGGCTTAGATCTGTTCAGTTATGGAATTCAGATCCTTCATCTGAAGAGGTAATAATAAAATCATAGCTTATTTTTCAATTATTGTTGATTGTTGTTTTAGTTTTTTTACTTTTTGTTAAACTGAAGGGAACAAACAATGAATTATAAGTAATTGACCTTGTTTTGTTTTTGTGAAAAATTGTTTAGGATGTTACTAAGAAAACAGTGCCTGTGTTGGAATTGAAGAGAAATGGAAGTGGTGGTGGTGCTTTTCATCCATTCCATAAAGAAGATAGAGTTAACAACAAGACAAGTGAATTGTTAAGTAAAGGACAACCCTCTTCGACCGGTGTAGCTGCCGTGAGTTCTAATGCGGCTAATGTCACCAGTAACAATGTTGGAGACATCAAAAGAGACGAGAAAGAGGAAAAGAGAAAGCAGCGACGGTGCTGGTCGCAGGAGTTGCACAAGCGATTCTTGAAGGCGCTTCAGCAACTTGGTGGTGCAGATTGTGAGTATCTTAATATATACATCAAGTTCTGATTGTTATGTATTCATGTTGTTAATGTAATTAACTGCAAATAGAATAAATGTTTAATGTGTTTTTTGCTTCTGTTCTATAGGTGCCACACCAAAGCAAATCAGAGAGGTAATGAATGTTGATGGCCTCACAAATGATGAAGTCAAAAGCCATTTACAGGTTTGTAGATATGTTCTTCTTCCCAACTCTTTTGTTGTCAATTTTCATCTGAGTATTTGTTTTGATTTTGTGATTTCTATTATCTAGTTGAATCTTTTAGTTTTAACTGTTTCATCTTGTTTTAGTCCCTTTCAATTGCATAGGATTTCAAAACAAACGAGTGATAGTTTTTGTTGTTACAGAAGTTACAACAACAGTTTAATATCCTCAATTAAAATCAATTTTTTTTTCTTTCTGTTTTGCACAGAAATACCGGTTGCATACTAGAAGACCAAGTTCAACAAATAACGAAAGTGCCAATTCACAAACAGCAGCACCTTTTGTCCTTGTAGGCAACATTTTTGTCCAACAACAAGAGTATGGTGGGGTAGCTTCATCAACAACAACAGGGGAAATGACAAAAGTGGTTGCACCTTCTGGAATATATGCACCAGTAGCCACACATCCTCAAGTTGCTACAATTAAGAAACCAGAGTTCAAGAGGTTTGAGCATTCAATCTCAGATGAAAGGGGTAATAACAGTGAAGGTGCTGTTCATTCAAATTCACCTACATCTTCATCCTCTACACATACTACTACCAATACTTCCCCTGGTTATTGA
Protein sequence

>MS.gene001686.t1

MQRFKMGFSDYIQALEQERRKIQVFPKELPLSLELVTQAIEACRQQLSGTTTEYNLNGQSECSEQTTSTDGPVLEEFIPIKKRASSYSHEVFDDVEDYDQHFHHKQQKLSLDNKKKSDWLRSVQLWNSDPSSEEDVTKKTVPVLELKRNGSGGGAFHPFHKEDRVNNKTSELLSKGQPSSTGVAAVSSNAANVTSNNVGDIKRDEKEEKRKQRRCWSQELHKRFLKALQQLGGADCATPKQIREVMNVDGLTNDEVKSHLQKYRLHTRRPSSTNNESANSQTAAPFVLVGNIFVQQQEYGGVASSTTTGEMTKVVAPSGIYAPVATHPQVATIKKPEFKRFEHSISDERGNNSEGAVHSNSPTSSSSTHTTTNTSPGY