AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00169

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00169.t1 MTR_2g034280 91.122 597 33 1 1 597 1 577 0.0 1103
MS.gene00169.t1 MTR_2g034260 80.333 600 101 6 1 597 1 586 0.0 968
MS.gene00169.t1 MTR_2g034250 79.043 606 99 9 1 597 1 587 0.0 945
MS.gene00169.t1 MTR_7g109580 44.153 496 259 5 103 597 78 556 5.44e-135 405
MS.gene00169.t1 MTR_1g086970 44.286 490 242 9 117 597 13 480 7.49e-129 387
MS.gene00169.t1 MTR_3g065980 30.250 400 249 7 210 596 159 541 6.06e-57 201
MS.gene00169.t1 MTR_7g027190 30.198 404 249 11 209 595 286 673 3.05e-47 176
MS.gene00169.t1 MTR_8g442410 31.959 388 246 12 216 595 148 525 6.97e-44 164
MS.gene00169.t1 MTR_7g074650 31.989 372 229 11 227 595 442 792 1.60e-41 160
MS.gene00169.t1 MTR_4g076140 29.060 468 243 15 195 595 24 469 1.89e-41 156
MS.gene00169.t1 MTR_4g104020 28.148 405 266 12 205 595 127 520 1.30e-40 155
MS.gene00169.t1 MTR_4g064150 27.990 393 261 8 213 594 351 732 2.05e-37 148
MS.gene00169.t1 MTR_7g062120 27.763 389 263 8 214 595 363 740 1.15e-36 146
MS.gene00169.t1 MTR_3g022830 27.635 427 240 8 224 596 26 437 3.41e-36 141
MS.gene00169.t1 MTR_4g064200 27.013 385 259 9 218 594 279 649 3.51e-36 144
MS.gene00169.t1 MTR_4g102790 27.487 382 253 11 224 594 293 661 3.67e-36 144
MS.gene00169.t1 MTR_5g094450 26.893 383 265 9 220 595 158 532 7.90e-36 141
MS.gene00169.t1 MTR_2g026250 25.974 385 267 8 218 595 223 596 9.22e-36 142
MS.gene00169.t1 MTR_4g076020 28.312 385 217 8 227 595 88 429 1.04e-35 139
MS.gene00169.t1 MTR_1g069725 28.713 404 257 12 195 594 86 462 1.14e-35 140
MS.gene00169.t1 MTR_4g133660 24.479 384 272 8 218 594 170 542 1.60e-34 138
MS.gene00169.t1 MTR_3g056110 26.684 386 258 9 220 595 172 542 3.36e-34 137
MS.gene00169.t1 MTR_4g064160 25.700 393 256 8 218 594 311 683 7.93e-34 137
MS.gene00169.t1 MTR_4g064120 27.907 387 253 12 212 585 243 616 5.12e-33 134
MS.gene00169.t1 MTR_4g077760 26.440 382 259 9 220 595 190 555 9.90e-33 132
MS.gene00169.t1 MTR_2g097410 26.209 393 264 8 214 594 362 740 1.53e-32 133
MS.gene00169.t1 MTR_2g097463 26.042 384 263 8 218 594 285 654 2.20e-32 132
MS.gene00169.t1 MTR_5g097480 26.471 340 229 7 220 550 174 501 5.16e-32 130
MS.gene00169.t1 MTR_2g082090 24.170 542 363 16 88 594 50 578 8.39e-32 130
MS.gene00169.t1 MTR_2g097473 27.013 385 258 8 218 594 284 653 1.14e-31 130
MS.gene00169.t1 MTR_2g097390 24.093 386 275 7 215 594 267 640 1.65e-31 130
MS.gene00169.t1 MTR_4g064180 27.273 385 258 11 212 585 243 616 2.45e-31 129
MS.gene00169.t1 MTR_2g097350 25.130 386 271 7 215 594 266 639 2.47e-31 129
MS.gene00169.t1 MTR_2g097310 24.093 386 275 7 215 594 264 637 1.17e-30 127
MS.gene00169.t1 MTR_3g072710 25.954 393 277 8 213 594 110 499 1.21e-30 126
MS.gene00169.t1 MTR_2g097467 25.974 385 262 8 218 594 284 653 2.46e-30 126
MS.gene00169.t1 MTR_3g089055 26.098 387 251 11 224 595 204 570 2.57e-30 125
MS.gene00169.t1 MTR_3g089055 26.098 387 251 11 224 595 199 565 3.21e-30 125
MS.gene00169.t1 MTR_1g029420 25.323 387 254 9 224 595 226 592 1.40e-29 124
MS.gene00169.t1 MTR_1g096030 25.352 426 253 14 208 597 65 461 4.32e-27 115
MS.gene00169.t1 MTR_2g097380 24.586 362 226 10 239 594 240 560 6.12e-26 112
MS.gene00169.t1 MTR_4g097080 24.000 400 281 7 203 595 120 503 9.83e-26 111
MS.gene00169.t1 MTR_5g009080 31.907 257 157 7 189 444 25 264 1.41e-24 107
MS.gene00169.t1 MTR_5g009080 31.579 152 102 2 445 595 328 478 1.16e-19 92.8
MS.gene00169.t1 MTR_8g093070 27.068 399 248 15 210 594 126 495 1.11e-23 105
MS.gene00169.t1 MTR_5g058860 23.493 481 318 15 146 594 34 496 1.42e-23 105
MS.gene00169.t1 MTR_5g015490 24.872 390 261 11 224 595 115 490 6.27e-23 102
MS.gene00169.t1 MTR_7g057230 25.926 405 266 13 205 594 271 656 3.96e-21 98.2
MS.gene00169.t1 MTR_4g026485 24.672 381 262 10 219 595 265 624 1.42e-20 96.3
MS.gene00169.t1 MTR_2g099110 27.336 289 193 8 224 507 75 351 3.23e-20 94.4
MS.gene00169.t1 MTR_6g047750 26.076 395 245 16 222 595 294 662 4.97e-18 88.2
MS.gene00169.t1 MTR_0092s0100 24.074 378 269 8 219 595 368 728 1.09e-17 87.4
MS.gene00169.t1 MTR_0092s0100 24.074 378 269 8 219 595 371 731 1.21e-17 87.4
MS.gene00169.t1 MTR_8g077940 25.627 359 220 14 222 562 181 510 6.10e-13 72.0
MS.gene00169.t1 MTR_8g020840 19.701 401 288 11 216 596 163 549 1.00e-12 71.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00169.t1 AT3G03450 30.886 395 247 8 210 596 170 546 1.24e-57 202
MS.gene00169.t1 AT1G66350 30.491 387 244 8 212 596 144 507 9.29e-55 194
MS.gene00169.t1 AT5G17490 30.749 387 244 9 214 596 151 517 4.13e-53 190
MS.gene00169.t1 AT2G01570 30.256 390 251 7 210 596 211 582 4.97e-53 191
MS.gene00169.t1 AT1G14920 31.026 390 248 8 210 596 159 530 1.24e-52 189
MS.gene00169.t1 AT5G41920 32.143 392 239 12 210 595 30 400 2.63e-46 168
MS.gene00169.t1 AT3G54220 31.892 370 233 8 227 594 297 649 9.02e-43 163
MS.gene00169.t1 AT1G07530 28.463 397 265 7 206 594 379 764 1.07e-39 155
MS.gene00169.t1 AT2G29060 28.278 389 260 7 215 594 313 691 2.04e-38 151
MS.gene00169.t1 AT2G29060 28.278 389 260 7 215 594 276 654 2.26e-38 150
MS.gene00169.t1 AT5G59450 29.716 387 253 11 218 595 222 598 7.00e-38 149
MS.gene00169.t1 AT3G46600 29.659 381 245 10 224 595 5 371 8.95e-38 144
MS.gene00169.t1 AT3G46600 29.306 389 252 10 216 595 205 579 1.71e-37 147
MS.gene00169.t1 AT1G55580 27.952 415 250 10 220 595 41 445 2.15e-37 144
MS.gene00169.t1 AT3G46600 29.306 389 252 10 216 595 173 547 3.76e-37 145
MS.gene00169.t1 AT1G63100 25.250 400 264 10 215 594 269 653 6.34e-36 143
MS.gene00169.t1 AT1G63100 25.250 400 264 10 215 594 244 628 8.52e-36 142
MS.gene00169.t1 AT2G29065 25.326 383 266 8 224 594 253 627 1.80e-35 142
MS.gene00169.t1 AT2G04890 25.729 377 258 7 224 595 54 413 1.09e-34 136
MS.gene00169.t1 AT4G17230 26.166 386 260 9 220 595 155 525 1.49e-34 138
MS.gene00169.t1 AT4G17230 26.166 386 260 9 220 595 155 525 1.49e-34 138
MS.gene00169.t1 AT1G07520 25.441 397 277 9 209 594 140 528 3.14e-34 137
MS.gene00169.t1 AT1G07520 25.441 397 277 9 209 594 140 528 3.14e-34 137
MS.gene00169.t1 AT1G07520 25.441 397 277 9 209 594 305 693 4.09e-34 138
MS.gene00169.t1 AT1G07520 25.441 397 277 9 209 594 304 692 4.71e-34 138
MS.gene00169.t1 AT5G66770 26.700 397 263 10 211 595 204 584 1.08e-33 136
MS.gene00169.t1 AT5G48150 24.479 384 270 9 220 595 119 490 9.78e-33 132
MS.gene00169.t1 AT5G48150 24.479 384 270 9 220 595 119 490 9.78e-33 132
MS.gene00169.t1 AT5G48150 24.479 384 270 9 220 595 119 490 9.78e-33 132
MS.gene00169.t1 AT5G48150 24.479 384 270 9 220 595 119 490 9.78e-33 132
MS.gene00169.t1 AT3G50650 26.462 359 231 10 251 595 203 542 8.64e-31 127
MS.gene00169.t1 AT2G37650 26.357 387 269 6 214 595 326 701 9.69e-31 128
MS.gene00169.t1 AT2G37650 26.357 387 269 6 214 595 338 713 1.00e-30 128
MS.gene00169.t1 AT1G50600 24.868 378 267 7 224 595 160 526 3.12e-30 125
MS.gene00169.t1 AT1G50600 24.868 378 267 7 224 595 231 597 8.42e-30 124
MS.gene00169.t1 AT1G50600 24.868 378 267 7 224 595 235 601 8.46e-30 124
MS.gene00169.t1 AT4G08250 26.034 411 262 12 204 594 88 476 7.82e-28 117
MS.gene00169.t1 AT1G21450 24.490 392 273 9 215 595 214 593 5.32e-23 103
MS.gene00169.t1 AT1G50420 30.451 266 166 8 187 445 15 268 4.65e-22 100
MS.gene00169.t1 AT1G50420 31.410 156 95 4 446 595 330 479 2.83e-18 88.6
MS.gene00169.t1 AT2G45160 25.414 362 242 10 241 595 299 639 2.78e-21 98.6
MS.gene00169.t1 AT4G00150 26.357 387 241 13 218 595 203 554 2.48e-20 95.5
MS.gene00169.t1 AT3G60630 26.246 301 201 7 306 595 332 622 3.84e-20 95.1
MS.gene00169.t1 AT4G37650 22.642 424 281 14 198 595 127 529 7.76e-19 90.5
MS.gene00169.t1 AT3G49950 24.359 390 263 12 224 594 31 407 4.59e-18 87.4
MS.gene00169.t1 AT5G52510 22.222 396 264 9 222 595 267 640 3.46e-16 82.4
MS.gene00169.t1 AT3G13840 25.887 282 183 10 221 490 145 412 4.44e-11 65.9

Find 73 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCTTCTTTGCATCTTTA+AGG 0.061011 2.1:-58402358 None:intergenic
AATATCTCGACTGGTGTTAA+AGG 0.227086 2.1:-58401756 None:intergenic
TGTACTTTAGTTATGGTATT+AGG 0.229731 2.1:+58402900 MS.gene00169:CDS
CTTTGAAGTGTATGTGTTTC+CGG 0.229764 2.1:-58402492 None:intergenic
CTGTTTCTTTGTCGATTCTT+TGG 0.234871 2.1:-58402181 None:intergenic
TTATGGTATTAGGAATATTT+TGG 0.239748 2.1:+58402910 MS.gene00169:CDS
TTCACTTGCTGAATCACTTT+TGG 0.248339 2.1:+58402028 MS.gene00169:CDS
TTGATTCTTAATCACCCTTT+TGG 0.256538 2.1:+58401963 MS.gene00169:CDS
GAGTCTCTTTGCTCTAATTT+TGG 0.258364 2.1:+58401531 MS.gene00169:CDS
CAAACAGAAACAGAGTTAAT+TGG 0.268686 2.1:-58403133 None:intergenic
ATGGAAAACTTGTGGGAATT+TGG 0.283067 2.1:+58401375 MS.gene00169:CDS
AGATCTTTGCTAACCTCTTC+AGG 0.290024 2.1:-58402251 None:intergenic
ATAATTGTATTGAACGAAAA+AGG 0.305796 2.1:-58402572 None:intergenic
AAAGTTTCATAATCTTGATA+TGG 0.312751 2.1:-58401627 None:intergenic
TCTTCCTCAAAATCTATATC+AGG 0.317310 2.1:+58401939 MS.gene00169:CDS
TTGTGGGAATTTGGTGAATT+CGG 0.328885 2.1:+58401384 MS.gene00169:CDS
TAATGAACCACACGCTTAAC+GGG 0.334947 2.1:-58402140 None:intergenic
CAATTAACTCTGTTTCTGTT+TGG 0.337936 2.1:+58403134 MS.gene00169:CDS
GAATTTGGTGAATTCGGTTT+TGG 0.347113 2.1:+58401390 MS.gene00169:CDS
ATAATGAACCACACGCTTAA+CGG 0.369400 2.1:-58402141 None:intergenic
TCGATTGAAGCGCTTGCATT+AGG 0.377874 2.1:-58402424 None:intergenic
ACTAAATCAAAATCATTAAC+AGG 0.380198 2.1:-58401837 None:intergenic
AACATTGAAACTTTAACAAA+TGG 0.394609 2.1:-58402308 None:intergenic
AATGGTCATTGTCTTCTTGT+TGG 0.397166 2.1:+58403097 MS.gene00169:CDS
TCTTGCACATTTGATATGAA+TGG 0.412387 2.1:+58403079 MS.gene00169:CDS
CAAAGAATCGACAAAGAAAC+AGG 0.418322 2.1:+58402182 MS.gene00169:CDS
GTCTTTGTATCAAGCTGAAT+TGG 0.419059 2.1:+58403030 MS.gene00169:CDS
AATGAGAGTCAGTTGTCAAC+TGG 0.426616 2.1:+58401873 MS.gene00169:CDS
GATTCTTTGGCAAAGTGCTT+CGG 0.434208 2.1:-58402168 None:intergenic
GAATCACTGTACTTTAGTTA+TGG 0.443550 2.1:+58402893 MS.gene00169:CDS
ATTGATCTCGAAATTCGAAA+AGG 0.451962 2.1:+58402386 MS.gene00169:CDS
GAAGATCTAGGAGACTCTAA+TGG 0.460843 2.1:+58401504 MS.gene00169:CDS
CAAAATGGAAGAATTCTTGT+AGG 0.470211 2.1:-58401696 None:intergenic
TTCTGTGATGGAAAACTTGT+GGG 0.472151 2.1:+58401368 None:intergenic
TGTCTTCTTGTTGGATGGAA+GGG 0.477569 2.1:+58403106 MS.gene00169:CDS
CAAAATCATTAACAGGATGA+TGG 0.479763 2.1:-58401830 None:intergenic
TCGGCTACGACCATTACTAT+AGG 0.488277 2.1:-58402743 None:intergenic
TTTCATAATCTTGATATGGT+TGG 0.489594 2.1:-58401623 None:intergenic
TTTCTCTTCATCAGAGAGAC+CGG 0.489642 2.1:-58401997 None:intergenic
AACTCTTTATCTTCGAAGAC+AGG 0.490618 2.1:+58402113 MS.gene00169:CDS
GGAGATATCATGAAGATAGC+TGG 0.498257 2.1:+58401894 MS.gene00169:CDS
TTGTCTTCTTGTTGGATGGA+AGG 0.499303 2.1:+58403105 MS.gene00169:CDS
TTGATCTCGAAATTCGAAAA+GGG 0.502199 2.1:+58402387 MS.gene00169:CDS
TTTCATTGTATCAAGTCCCT+TGG 0.502952 2.1:-58401460 None:intergenic
CCGGAGAAAGAAAATCCAAA+AGG 0.504198 2.1:-58401978 None:intergenic
GTCATTGTCTTCTTGTTGGA+TGG 0.509549 2.1:+58403101 MS.gene00169:CDS
CTCTCATGACTGTTTCAAGT+TGG 0.524579 2.1:-58402703 None:intergenic
AAATTCGAAAAGGGTTACAT+TGG 0.529055 2.1:+58402396 MS.gene00169:CDS
AGAAGCTCATCTTCTTGTGA+TGG 0.534656 2.1:-58401570 None:intergenic
ATCTTTATTTGATCCTGAAG+AGG 0.540063 2.1:+58402238 MS.gene00169:CDS
ACTTGAGCTTCTAAAGATAA+CGG 0.547553 2.1:+58402460 MS.gene00169:CDS
AACTATAAATCCTATAGTAA+TGG 0.549134 2.1:+58402733 MS.gene00169:CDS
CGGAGAAAGAAAATCCAAAA+GGG 0.549231 2.1:-58401977 None:intergenic
GTTATCTTTAGAAGCTCAAG+TGG 0.552221 2.1:-58402458 None:intergenic
AAGATAACGGCTATTGCCAC+CGG 0.561951 2.1:+58402473 MS.gene00169:CDS
TCGACTGGTGTTAAAGGCGA+TGG 0.563201 2.1:-58401750 None:intergenic
TGTTTGGAAGATTGTATGAA+AGG 0.565315 2.1:+58402845 MS.gene00169:CDS
AAGAGGTTATCACCTGATGA+AGG 0.566194 2.1:+58401801 MS.gene00169:CDS
TGATAACTTCAACATTGGCA+TGG 0.568588 2.1:+58401650 MS.gene00169:CDS
ATTGTGATTAGCTTCAGTCT+CGG 0.571904 2.1:-58402762 None:intergenic
ATTCTGTGATGGAAAACTTG+TGG 0.579437 2.1:+58401367 None:intergenic
CTGGTGTTAAAGGCGATGGT+TGG 0.604359 2.1:-58401746 None:intergenic
GTTGCTAAAAGATTTCCTTG+TGG 0.611038 2.1:+58403052 MS.gene00169:CDS
AAGCTTATAGTTGAAGACAC+TGG 0.619738 2.1:+58402512 MS.gene00169:CDS
AGGTAACCCCGTTAAGCGTG+TGG 0.631363 2.1:+58402133 MS.gene00169:CDS
AAATTGAGAGTAAACTGCAA+CGG 0.631573 2.1:-58402651 None:intergenic
TCATGAAGATAGCTGGAACA+AGG 0.635820 2.1:+58401901 MS.gene00169:CDS
ATGATACTTCTGTTGATCAG+TGG 0.638562 2.1:-58401598 None:intergenic
CGAATGTGAAGATTGATGTG+TGG 0.657727 2.1:+58402957 MS.gene00169:CDS
ATTATGGCAAAGGATTCAAG+AGG 0.672484 2.1:+58401784 MS.gene00169:CDS
TGAAGTGTATGTGTTTCCGG+TGG 0.687997 2.1:-58402489 None:intergenic
AATGAACCACACGCTTAACG+GGG 0.713768 2.1:-58402139 None:intergenic
AATGTGCAAGAATTTCCACA+AGG 0.746520 2.1:-58403067 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTTTCATAATCTTGATA+TGG - chr2.1:58401630-58401649 None:intergenic 20.0%
!! AACATTGAAACTTTAACAAA+TGG - chr2.1:58402311-58402330 None:intergenic 20.0%
!! AACTATAAATCCTATAGTAA+TGG + chr2.1:58402733-58402752 MS.gene00169:CDS 20.0%
!! ACTAAATCAAAATCATTAAC+AGG - chr2.1:58401840-58401859 None:intergenic 20.0%
!! ATAATTGTATTGAACGAAAA+AGG - chr2.1:58402575-58402594 None:intergenic 20.0%
!! ATATTGAAAAATTATGGCAA+AGG + chr2.1:58401774-58401793 MS.gene00169:CDS 20.0%
!!! TTATGGTATTAGGAATATTT+TGG + chr2.1:58402910-58402929 MS.gene00169:CDS 20.0%
! GTCGAGATATTGAAAAATTA+TGG + chr2.1:58401768-58401787 MS.gene00169:CDS 25.0%
! TGTACTTTAGTTATGGTATT+AGG + chr2.1:58402900-58402919 MS.gene00169:CDS 25.0%
! TTTCATAATCTTGATATGGT+TGG - chr2.1:58401626-58401645 None:intergenic 25.0%
!! ATTTTTCACAAGATTTGGAA+TGG + chr2.1:58402985-58403004 MS.gene00169:CDS 25.0%
!!! ACTTTTGATAACTTCAACAT+TGG + chr2.1:58401645-58401664 MS.gene00169:CDS 25.0%
!!! TAATTTTTCAATATCTCGAC+TGG - chr2.1:58401768-58401787 None:intergenic 25.0%
!!! TTCATCCAATGATACTTTTT+GGG + chr2.1:58401434-58401453 MS.gene00169:CDS 25.0%
AAATTCGAAAAGGGTTACAT+TGG + chr2.1:58402396-58402415 MS.gene00169:CDS 30.0%
AAATTGAGAGTAAACTGCAA+CGG - chr2.1:58402654-58402673 None:intergenic 30.0%
ATTGATCTCGAAATTCGAAA+AGG + chr2.1:58402386-58402405 MS.gene00169:CDS 30.0%
CAAAATCATTAACAGGATGA+TGG - chr2.1:58401833-58401852 None:intergenic 30.0%
CAAAATGGAAGAATTCTTGT+AGG - chr2.1:58401699-58401718 None:intergenic 30.0%
CAAACAGAAACAGAGTTAAT+TGG - chr2.1:58403136-58403155 None:intergenic 30.0%
CTCTTCGAAAAATGCAATAA+GGG - chr2.1:58402285-58402304 None:intergenic 30.0%
GAATCACTGTACTTTAGTTA+TGG + chr2.1:58402893-58402912 MS.gene00169:CDS 30.0%
GAATCTTCTTTGCATCTTTA+AGG - chr2.1:58402361-58402380 None:intergenic 30.0%
TCTTCCTCAAAATCTATATC+AGG + chr2.1:58401939-58401958 MS.gene00169:CDS 30.0%
TCTTGCACATTTGATATGAA+TGG + chr2.1:58403079-58403098 MS.gene00169:CDS 30.0%
TGTTTGGAAGATTGTATGAA+AGG + chr2.1:58402845-58402864 MS.gene00169:CDS 30.0%
TTGATCTCGAAATTCGAAAA+GGG + chr2.1:58402387-58402406 MS.gene00169:CDS 30.0%
! ACTTGAGCTTCTAAAGATAA+CGG + chr2.1:58402460-58402479 MS.gene00169:CDS 30.0%
! ATCTTTATTTGATCCTGAAG+AGG + chr2.1:58402238-58402257 MS.gene00169:CDS 30.0%
! CAAACCTGATATAGATTTTG+AGG - chr2.1:58401946-58401965 None:intergenic 30.0%
! GTTCATCCAATGATACTTTT+TGG + chr2.1:58401433-58401452 MS.gene00169:CDS 30.0%
! TCCATTTTGTGACACAAAAA+AGG + chr2.1:58401710-58401729 MS.gene00169:CDS 30.0%
! TTGATTCTTAATCACCCTTT+TGG + chr2.1:58401963-58401982 MS.gene00169:CDS 30.0%
! TTTTCTGTAGAAGAAGATCT+AGG + chr2.1:58401492-58401511 MS.gene00169:CDS 30.0%
!! AAAAAAGTGCTTCGATGAAA+CGG - chr2.1:58402805-58402824 None:intergenic 30.0%
!! AGAGCATTTTTCACAAGATT+TGG + chr2.1:58402980-58402999 MS.gene00169:CDS 30.0%
!! CAATTAACTCTGTTTCTGTT+TGG + chr2.1:58403134-58403153 MS.gene00169:CDS 30.0%
!!! ATTAGGAATATTTTGGCTGA+AGG + chr2.1:58402917-58402936 MS.gene00169:CDS 30.0%
!!! TCCTTTTTTGTGTCACAAAA+TGG - chr2.1:58401714-58401733 None:intergenic 30.0%
AACTCTTTATCTTCGAAGAC+AGG + chr2.1:58402113-58402132 MS.gene00169:CDS 35.0%
AAGCTTATAGTTGAAGACAC+TGG + chr2.1:58402512-58402531 MS.gene00169:CDS 35.0%
AATGGTCATTGTCTTCTTGT+TGG + chr2.1:58403097-58403116 MS.gene00169:CDS 35.0%
AATGTGCAAGAATTTCCACA+AGG - chr2.1:58403070-58403089 None:intergenic 35.0%
ATAATGAACCACACGCTTAA+CGG - chr2.1:58402144-58402163 None:intergenic 35.0%
ATGATACTTCTGTTGATCAG+TGG - chr2.1:58401601-58401620 None:intergenic 35.0%
ATGGAAAACTTGTGGGAATT+TGG + chr2.1:58401375-58401394 MS.gene00169:CDS 35.0%
ATTATGGCAAAGGATTCAAG+AGG + chr2.1:58401784-58401803 MS.gene00169:CDS 35.0%
CAAAGAATCGACAAAGAAAC+AGG + chr2.1:58402182-58402201 MS.gene00169:CDS 35.0%
CGGAGAAAGAAAATCCAAAA+GGG - chr2.1:58401980-58401999 None:intergenic 35.0%
GCTCTTCGAAAAATGCAATA+AGG - chr2.1:58402286-58402305 None:intergenic 35.0%
GTCTTTGTATCAAGCTGAAT+TGG + chr2.1:58403030-58403049 MS.gene00169:CDS 35.0%
GTTGCTAAAAGATTTCCTTG+TGG + chr2.1:58403052-58403071 MS.gene00169:CDS 35.0%
TCGAAAAATGCAATAAGGGT+TGG - chr2.1:58402281-58402300 None:intergenic 35.0%
TGAAGACACTGGCAAAAAAT+TGG + chr2.1:58402523-58402542 MS.gene00169:CDS 35.0%
TGATAACTTCAACATTGGCA+TGG + chr2.1:58401650-58401669 MS.gene00169:CDS 35.0%
TTTCATTGTATCAAGTCCCT+TGG - chr2.1:58401463-58401482 None:intergenic 35.0%
! AATATCTCGACTGGTGTTAA+AGG - chr2.1:58401759-58401778 None:intergenic 35.0%
! CCTTTTGGATTTTCTTTCTC+CGG + chr2.1:58401978-58401997 MS.gene00169:CDS 35.0%
! GAGTCTCTTTGCTCTAATTT+TGG + chr2.1:58401531-58401550 MS.gene00169:CDS 35.0%
! GTTATCTTTAGAAGCTCAAG+TGG - chr2.1:58402461-58402480 None:intergenic 35.0%
! TTGTGGGAATTTGGTGAATT+CGG + chr2.1:58401384-58401403 MS.gene00169:CDS 35.0%
!! ATTGTGATTAGCTTCAGTCT+CGG - chr2.1:58402765-58402784 None:intergenic 35.0%
!! CTGTTTCTTTGTCGATTCTT+TGG - chr2.1:58402184-58402203 None:intergenic 35.0%
!! CTTTGAAGTGTATGTGTTTC+CGG - chr2.1:58402495-58402514 None:intergenic 35.0%
!! GAATTTGGTGAATTCGGTTT+TGG + chr2.1:58401390-58401409 MS.gene00169:CDS 35.0%
!! GGTATCCCAAAAAGTATCAT+TGG - chr2.1:58401442-58401461 None:intergenic 35.0%
!! TAGTGCGCTTTTTGATTGTT+TGG + chr2.1:58402829-58402848 MS.gene00169:CDS 35.0%
!! TCTTTTCGAGAAAGATTCTG+AGG + chr2.1:58402625-58402644 MS.gene00169:CDS 35.0%
!! TTCACTTGCTGAATCACTTT+TGG + chr2.1:58402028-58402047 MS.gene00169:CDS 35.0%
!!! GATACTTTTTGGGATACCAA+GGG + chr2.1:58401444-58401463 MS.gene00169:CDS 35.0%
!!! TGATACTTTTTGGGATACCA+AGG + chr2.1:58401443-58401462 MS.gene00169:CDS 35.0%
!!! TGATGGATTTTTCCTTCATC+AGG - chr2.1:58401816-58401835 None:intergenic 35.0%
AAGAGGTTATCACCTGATGA+AGG + chr2.1:58401801-58401820 MS.gene00169:CDS 40.0%
AATGAGAGTCAGTTGTCAAC+TGG + chr2.1:58401873-58401892 MS.gene00169:CDS 40.0%
AGAAGCTCATCTTCTTGTGA+TGG - chr2.1:58401573-58401592 None:intergenic 40.0%
AGACACTGGCAAAAAATTGG+AGG + chr2.1:58402526-58402545 MS.gene00169:CDS 40.0%
AGATCTTTGCTAACCTCTTC+AGG - chr2.1:58402254-58402273 None:intergenic 40.0%
CCGGAGAAAGAAAATCCAAA+AGG - chr2.1:58401981-58402000 None:intergenic 40.0%
CGAATGTGAAGATTGATGTG+TGG + chr2.1:58402957-58402976 MS.gene00169:CDS 40.0%
GAAGATCTAGGAGACTCTAA+TGG + chr2.1:58401504-58401523 MS.gene00169:CDS 40.0%
GGAGATATCATGAAGATAGC+TGG + chr2.1:58401894-58401913 MS.gene00169:CDS 40.0%
GTCATTGTCTTCTTGTTGGA+TGG + chr2.1:58403101-58403120 MS.gene00169:CDS 40.0%
TAATGAACCACACGCTTAAC+GGG - chr2.1:58402143-58402162 None:intergenic 40.0%
TCATGAAGATAGCTGGAACA+AGG + chr2.1:58401901-58401920 MS.gene00169:CDS 40.0%
TGTCTTCTTGTTGGATGGAA+GGG + chr2.1:58403106-58403125 MS.gene00169:CDS 40.0%
TTGTCTTCTTGTTGGATGGA+AGG + chr2.1:58403105-58403124 MS.gene00169:CDS 40.0%
TTTCTCTTCATCAGAGAGAC+CGG - chr2.1:58402000-58402019 None:intergenic 40.0%
! CTCTCATGACTGTTTCAAGT+TGG - chr2.1:58402706-58402725 None:intergenic 40.0%
! GATTCTTTGGCAAAGTGCTT+CGG - chr2.1:58402171-58402190 None:intergenic 40.0%
!! ATTCGGTTTTGGTGTAACTG+AGG + chr2.1:58401401-58401420 MS.gene00169:CDS 40.0%
AATGAACCACACGCTTAACG+GGG - chr2.1:58402142-58402161 None:intergenic 45.0%
TCGGCTACGACCATTACTAT+AGG - chr2.1:58402746-58402765 None:intergenic 45.0%
TGAAGTGTATGTGTTTCCGG+TGG - chr2.1:58402492-58402511 None:intergenic 45.0%
! AAGATAACGGCTATTGCCAC+CGG + chr2.1:58402473-58402492 MS.gene00169:CDS 45.0%
! TCGATTGAAGCGCTTGCATT+AGG - chr2.1:58402427-58402446 None:intergenic 45.0%
!! TTGGCTTGCGCTGAAAAAGT+TGG + chr2.1:58402047-58402066 MS.gene00169:CDS 45.0%
! CTGGTGTTAAAGGCGATGGT+TGG - chr2.1:58401749-58401768 None:intergenic 50.0%
! TCGACTGGTGTTAAAGGCGA+TGG - chr2.1:58401753-58401772 None:intergenic 50.0%
AGGTAACCCCGTTAAGCGTG+TGG + chr2.1:58402133-58402152 MS.gene00169:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 58401375 58403168 58401375 ID=MS.gene00169
chr2.1 mRNA 58401375 58403168 58401375 ID=MS.gene00169.t1;Parent=MS.gene00169
chr2.1 exon 58401375 58403168 58401375 ID=MS.gene00169.t1.exon1;Parent=MS.gene00169.t1
chr2.1 CDS 58401375 58403168 58401375 ID=cds.MS.gene00169.t1;Parent=MS.gene00169.t1
Gene Sequence

>MS.gene00169

ATGGAAAACTTGTGGGAATTTGGTGAATTCGGTTTTGGTGTAACTGAGGATAAACTCAGTTCATCCAATGATACTTTTTGGGATACCAAGGGACTTGATACAATGAAAAATGTTCAATTTTCTGTAGAAGAAGATCTAGGAGACTCTAATGGAATTGAGTCTCTTTGCTCTAATTTTGGTCTCTTTCAAGATGATCCATCACAAGAAGATGAGCTTCTTCTTTCCACTGATCAACAGAAGTATCATCACCAACCATATCAAGATTATGAAACTTTTGATAACTTCAACATTGGCATGGTTCACTTTGATGAACAACAATGTCCTACAAGAATTCTTCCATTTTGTGACACAAAAAAGGACAATCAATATTACCAACCATCGCCTTTAACACCAGTCGAGATATTGAAAAATTATGGCAAAGGATTCAAGAGGTTATCACCTGATGAAGGAAAAATCCATCATCCTGTTAATGATTTTGATTTAGTTACAGATAATCAAAATGAGAGTCAGTTGTCAACTGGAGATATCATGAAGATAGCTGGAACAAGGTTCATACAATCATCATCTTCCTCAAAATCTATATCAGGTTTGATTCTTAATCACCCTTTTGGATTTTCTTTCTCCGGTCTCTCTGATGAAGAGAAAGAAGATGTTTCACTTGCTGAATCACTTTTGGCTTGCGCTGAAAAAGTTGGATATCAACAATTTGAACGCGCTAGAAAATTGTTATCACATATCAACTCTTTATCTTCGAAGACAGGTAACCCCGTTAAGCGTGTGGTTCATTATTTTGCCGAAGCACTTTGCCAAAGAATCGACAAAGAAACAGGAAGAGTTTCATCTAACAATACGCAAAAGATAGAATCTTTATTTGATCCTGAAGAGGTTAGCAAAGATCTAAATCCAACCCTTATTGCATTTTTCGAAGAGCTTCCATTTGTTAAAGTTTCAATGTTCACATGTGTACAAGCTTTAATAGAAAACCTTAAAGATGCAAAGAAGATTCATGTTATTGATCTCGAAATTCGAAAAGGGTTACATTGGACAATCCTAATGCAAGCGCTTCAATCGAGAACAGAATGTCCACTTGAGCTTCTAAAGATAACGGCTATTGCCACCGGAAACACATACACTTCAAAGCTTATAGTTGAAGACACTGGCAAAAAATTGGAGGATTTTGCACAAAGTTTGAACATACCTTTTTCGTTCAATACAATTATTGTATCTAACTTGTTACATCTAAGAGAAGATCTTTTCGAGAAAGATTCTGAGGAAACCGTTGCAGTTTACTCTCAATTTGCTCTTAGAAGCAGTATTCAACAATCAGACCAACTTGAAACAGTCATGAGAGTTGTTAGAACTATAAATCCTATAGTAATGGTCGTAGCCGAGACTGAAGCTAATCACAATTCTAAATCTTTTGTGAACCGTTTCATCGAAGCACTTTTTTACTTTAGTGCGCTTTTTGATTGTTTGGAAGATTGTATGAAAGGTGATGAGAAGAACAGAATGATTATAGAATCACTGTACTTTAGTTATGGTATTAGGAATATTTTGGCTGAAGGAGTTGAAAGAAAGAGTACGAATGTGAAGATTGATGTGTGGAGAGCATTTTTCACAAGATTTGGAATGGTTGAAACAGAATTGAGTATGAAGTCTTTGTATCAAGCTGAATTGGTTGCTAAAAGATTTCCTTGTGGAAATTCTTGCACATTTGATATGAATGGTCATTGTCTTCTTGTTGGATGGAAGGGAACACCAATTAACTCTGTTTCTGTTTGGAAATTCCTTTGA
Protein sequence

>MS.gene00169.t1

MENLWEFGEFGFGVTEDKLSSSNDTFWDTKGLDTMKNVQFSVEEDLGDSNGIESLCSNFGLFQDDPSQEDELLLSTDQQKYHHQPYQDYETFDNFNIGMVHFDEQQCPTRILPFCDTKKDNQYYQPSPLTPVEILKNYGKGFKRLSPDEGKIHHPVNDFDLVTDNQNESQLSTGDIMKIAGTRFIQSSSSSKSISGLILNHPFGFSFSGLSDEEKEDVSLAESLLACAEKVGYQQFERARKLLSHINSLSSKTGNPVKRVVHYFAEALCQRIDKETGRVSSNNTQKIESLFDPEEVSKDLNPTLIAFFEELPFVKVSMFTCVQALIENLKDAKKIHVIDLEIRKGLHWTILMQALQSRTECPLELLKITAIATGNTYTSKLIVEDTGKKLEDFAQSLNIPFSFNTIIVSNLLHLREDLFEKDSEETVAVYSQFALRSSIQQSDQLETVMRVVRTINPIVMVVAETEANHNSKSFVNRFIEALFYFSALFDCLEDCMKGDEKNRMIIESLYFSYGIRNILAEGVERKSTNVKIDVWRAFFTRFGMVETELSMKSLYQAELVAKRFPCGNSCTFDMNGHCLLVGWKGTPINSVSVWKFL