Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001795.t1 | XP_013444885.1 | 98.8 | 160 | 2 | 0 | 1 | 160 | 46 | 205 | 5.90E-84 | 320.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001795.t1 | Q1ECF5 | 65.2 | 155 | 54 | 0 | 1 | 155 | 46 | 200 | 5.5e-54 | 211.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001795.t1 | A0A072TZG0 | 98.8 | 160 | 2 | 0 | 1 | 160 | 46 | 205 | 4.3e-84 | 320.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene001795.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001795.t1 | MTR_8g031490 | 98.750 | 160 | 2 | 0 | 1 | 160 | 46 | 205 | 1.02e-116 | 328 |
| MS.gene001795.t1 | MTR_8g031490 | 53.659 | 82 | 37 | 1 | 52 | 132 | 3 | 84 | 1.50e-24 | 94.7 |
| MS.gene001795.t1 | MTR_7g076720 | 72.840 | 162 | 42 | 2 | 1 | 160 | 46 | 207 | 3.78e-78 | 231 |
| MS.gene001795.t1 | MTR_7g076720 | 57.746 | 71 | 30 | 0 | 52 | 122 | 3 | 73 | 4.10e-24 | 93.6 |
| MS.gene001795.t1 | MTR_3g070050 | 60.563 | 142 | 50 | 2 | 1 | 136 | 4 | 145 | 4.00e-60 | 183 |
| MS.gene001795.t1 | MTR_3g070050 | 60.563 | 142 | 50 | 2 | 1 | 136 | 46 | 187 | 2.41e-59 | 182 |
| MS.gene001795.t1 | MTR_3g070050 | 53.521 | 71 | 33 | 0 | 52 | 122 | 3 | 73 | 5.07e-22 | 87.8 |
| MS.gene001795.t1 | MTR_8g097300 | 54.430 | 158 | 66 | 3 | 1 | 157 | 46 | 198 | 4.03e-54 | 170 |
| MS.gene001795.t1 | MTR_8g097300 | 42.424 | 66 | 38 | 0 | 52 | 117 | 3 | 68 | 6.37e-15 | 69.3 |
| MS.gene001795.t1 | MTR_1g017950 | 45.333 | 150 | 75 | 3 | 1 | 144 | 46 | 194 | 3.53e-40 | 134 |
| MS.gene001795.t1 | MTR_1g017950 | 51.471 | 68 | 33 | 0 | 52 | 119 | 3 | 70 | 1.45e-18 | 78.6 |
| MS.gene001795.t1 | MTR_4g106800 | 45.185 | 135 | 61 | 2 | 1 | 126 | 47 | 177 | 6.44e-37 | 125 |
| MS.gene001795.t1 | MTR_4g106800 | 47.059 | 68 | 36 | 0 | 52 | 119 | 4 | 71 | 1.83e-16 | 72.8 |
| MS.gene001795.t1 | MTR_3g070033 | 56.923 | 65 | 28 | 0 | 72 | 136 | 6 | 70 | 1.16e-24 | 90.9 |
| MS.gene001795.t1 | MTR_1g106100 | 35.849 | 106 | 66 | 1 | 14 | 117 | 20 | 125 | 2.26e-18 | 77.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001795.t1 | AT3G61230 | 67.532 | 154 | 46 | 2 | 1 | 150 | 47 | 200 | 1.43e-74 | 222 |
| MS.gene001795.t1 | AT3G61230 | 44.898 | 98 | 50 | 1 | 50 | 147 | 2 | 95 | 2.83e-25 | 96.7 |
| MS.gene001795.t1 | AT1G01780 | 65.161 | 155 | 54 | 0 | 1 | 155 | 46 | 200 | 1.01e-71 | 214 |
| MS.gene001795.t1 | AT1G01780 | 45.833 | 96 | 46 | 1 | 52 | 147 | 3 | 92 | 2.23e-24 | 94.4 |
| MS.gene001795.t1 | AT2G45800 | 66.667 | 159 | 47 | 4 | 1 | 153 | 15 | 173 | 1.23e-71 | 214 |
| MS.gene001795.t1 | AT2G45800 | 66.667 | 159 | 47 | 4 | 1 | 153 | 46 | 204 | 6.02e-71 | 213 |
| MS.gene001795.t1 | AT2G45800 | 60.563 | 71 | 28 | 0 | 52 | 122 | 3 | 73 | 7.59e-26 | 98.6 |
| MS.gene001795.t1 | AT3G55770 | 67.424 | 132 | 39 | 2 | 1 | 128 | 46 | 177 | 1.19e-63 | 194 |
| MS.gene001795.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 52 | 149 | 3 | 98 | 2.53e-20 | 83.6 |
| MS.gene001795.t1 | AT3G55770 | 67.424 | 132 | 39 | 2 | 1 | 128 | 46 | 177 | 1.19e-63 | 194 |
| MS.gene001795.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 52 | 149 | 3 | 98 | 2.53e-20 | 83.6 |
| MS.gene001795.t1 | AT3G55770 | 67.424 | 132 | 39 | 2 | 1 | 128 | 46 | 177 | 1.19e-63 | 194 |
| MS.gene001795.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 52 | 149 | 3 | 98 | 2.53e-20 | 83.6 |
| MS.gene001795.t1 | AT3G55770 | 67.424 | 132 | 39 | 2 | 1 | 128 | 46 | 177 | 1.19e-63 | 194 |
| MS.gene001795.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 52 | 149 | 3 | 98 | 2.53e-20 | 83.6 |
| MS.gene001795.t1 | AT2G39900 | 66.165 | 133 | 40 | 2 | 1 | 128 | 46 | 178 | 3.54e-63 | 192 |
| MS.gene001795.t1 | AT2G39900 | 48.101 | 79 | 41 | 0 | 52 | 130 | 3 | 81 | 8.85e-21 | 84.7 |
| MS.gene001795.t1 | AT3G55770 | 53.614 | 166 | 39 | 3 | 1 | 128 | 46 | 211 | 7.23e-56 | 175 |
| MS.gene001795.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 52 | 149 | 3 | 98 | 6.82e-20 | 83.2 |
| MS.gene001795.t1 | AT1G10200 | 45.385 | 130 | 65 | 2 | 1 | 124 | 4 | 133 | 2.87e-38 | 128 |
| MS.gene001795.t1 | AT1G10200 | 45.385 | 130 | 65 | 2 | 1 | 124 | 46 | 175 | 3.06e-37 | 126 |
| MS.gene001795.t1 | AT1G10200 | 50.000 | 68 | 34 | 0 | 52 | 119 | 3 | 70 | 1.97e-17 | 75.9 |
| MS.gene001795.t1 | AT3G55770 | 64.773 | 88 | 26 | 3 | 1 | 84 | 46 | 132 | 2.16e-31 | 110 |
| MS.gene001795.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 52 | 149 | 3 | 98 | 4.69e-21 | 84.0 |
| MS.gene001795.t1 | AT3G55770 | 65.854 | 82 | 22 | 3 | 1 | 76 | 46 | 127 | 9.26e-29 | 103 |
| MS.gene001795.t1 | AT3G55770 | 42.857 | 98 | 54 | 1 | 52 | 149 | 3 | 98 | 2.61e-21 | 84.3 |
Find 30 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCATTCAAGAGGTTTCTTC+TGG | 0.235129 | 8.3:+63482321 | None:intergenic |
| GTTTCTTCTGGATGATCTTC+AGG | 0.252303 | 8.3:+63482333 | None:intergenic |
| TCAAGCTATGCTGCTCTTGA+TGG | 0.353414 | 8.3:-63482501 | MS.gene001795:CDS |
| CAAAATGAACCCTGCAGTAT+AGG | 0.377575 | 8.3:+63482475 | None:intergenic |
| AGCAAGTCGCACATTTGTCC+AGG | 0.436853 | 8.3:+63482910 | None:intergenic |
| TGCTTTAGGTGTGCTCATGC+AGG | 0.437095 | 8.3:-63482540 | MS.gene001795:CDS |
| TTGATGGTGTCCTATACTGC+AGG | 0.448405 | 8.3:-63482485 | MS.gene001795:CDS |
| TCTACCACAAGACATGCTTT+AGG | 0.453453 | 8.3:-63482554 | MS.gene001795:CDS |
| AACCGTCTATCCACTGGAAA+AGG | 0.456725 | 8.3:-63482883 | MS.gene001795:intron |
| CTCTAAAACCGTCTATCCAC+TGG | 0.500295 | 8.3:-63482889 | MS.gene001795:CDS |
| TTGTTACTGTTTCATTCAAG+AGG | 0.524503 | 8.3:+63482311 | None:intergenic |
| TTCCATGTTCAGTGGTACCC+TGG | 0.525905 | 8.3:-63482928 | MS.gene001795:CDS |
| AACATGGAAGATAGTCTACT+TGG | 0.545011 | 8.3:+63482942 | None:intergenic |
| GGTGCTTCTGCTGATTGTGA+AGG | 0.545791 | 8.3:+63482354 | None:intergenic |
| ATTAAACAGGTGACACTGGA+AGG | 0.549370 | 8.3:-63482582 | MS.gene001795:intron |
| GCAATAGAGAATTCCATCCA+TGG | 0.558350 | 8.3:+63483523 | None:intergenic |
| AGTACATACTCCTCCATGGA+TGG | 0.562913 | 8.3:-63483536 | MS.gene001795:CDS |
| TTCTGACAAAGCAAATGAGC+TGG | 0.565336 | 8.3:-63483299 | MS.gene001795:intron |
| TACTCAAATCTGCACACAAG+AGG | 0.573033 | 8.3:-63482416 | MS.gene001795:CDS |
| GCTTTAGGTGTGCTCATGCA+GGG | 0.590942 | 8.3:-63482539 | MS.gene001795:CDS |
| TGATGGTGTCCTATACTGCA+GGG | 0.598936 | 8.3:-63482484 | MS.gene001795:CDS |
| GATGAGTACATACTCCTCCA+TGG | 0.608526 | 8.3:-63483540 | MS.gene001795:CDS |
| GTCCAGGGTACCACTGAACA+TGG | 0.612761 | 8.3:+63482926 | None:intergenic |
| GCATAGCTTGAATGTGTGAG+AGG | 0.618734 | 8.3:+63482513 | None:intergenic |
| AAATCTGCACACAAGAGGAA+TGG | 0.618865 | 8.3:-63482411 | MS.gene001795:CDS |
| CATAGCTTGAATGTGTGAGA+GGG | 0.638300 | 8.3:+63482514 | None:intergenic |
| AGACTATCTTCCATGTTCAG+TGG | 0.654978 | 8.3:-63482936 | MS.gene001795:CDS |
| GCAAGTCGCACATTTGTCCA+GGG | 0.661497 | 8.3:+63482911 | None:intergenic |
| CACACCTAAAGCATGTCTTG+TGG | 0.697099 | 8.3:+63482550 | None:intergenic |
| ATAGAGAATTCCATCCATGG+AGG | 0.764020 | 8.3:+63483526 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAATAAAAATTCAAGTAA+TGG | + | chr8.3:63482635-63482654 | None:intergenic | 10.0% |
| !!! | AATTGTTTCTTTTAAAAATT+TGG | - | chr8.3:63483069-63483088 | MS.gene001795:intron | 10.0% |
| !!! | AATTCATCTCTAATATTTTA+TGG | + | chr8.3:63482733-63482752 | None:intergenic | 15.0% |
| !!! | ATATCATTTTTTGTTCATTT+TGG | - | chr8.3:63482510-63482529 | MS.gene001795:CDS | 15.0% |
| !!! | TTTTTTTGGTTTTATTAAAC+AGG | - | chr8.3:63483270-63483289 | MS.gene001795:intron | 15.0% |
| !! | AATATGGAATTCATAAATGA+AGG | - | chr8.3:63483208-63483227 | MS.gene001795:intron | 20.0% |
| !!! | AGACAAATTTCATATCTTTT+TGG | + | chr8.3:63482701-63482720 | None:intergenic | 20.0% |
| !!! | GCTTTTTTAAATCAAATTCT+TGG | + | chr8.3:63483122-63483141 | None:intergenic | 20.0% |
| ! | AATGATATATGTGTATCAAC+TGG | + | chr8.3:63482498-63482517 | None:intergenic | 25.0% |
| ! | TTACCTGATTTCATATAACT+TGG | - | chr8.3:63483095-63483114 | MS.gene001795:intron | 25.0% |
| ! | TTTAAACACAAGCTGTAATA+TGG | - | chr8.3:63483192-63483211 | MS.gene001795:intron | 25.0% |
| !! | AAAAAATGTGTTACGACTTT+TGG | + | chr8.3:63483256-63483275 | None:intergenic | 25.0% |
| !! | AACAATTTTCCACAAAATCA+AGG | + | chr8.3:63483056-63483075 | None:intergenic | 25.0% |
| !! | TCTTTCTAGTAGTGATATAA+AGG | - | chr8.3:63482795-63482814 | MS.gene001795:intron | 25.0% |
| !!! | AAAGTCGTAACACATTTTTT+TGG | - | chr8.3:63483256-63483275 | MS.gene001795:intron | 25.0% |
| !!! | GTATTGATCTTTTACAGAAT+AGG | - | chr8.3:63482897-63482916 | MS.gene001795:CDS | 25.0% |
| GAAAAAAGTTGAATTAGCCA+TGG | - | chr8.3:63482665-63482684 | MS.gene001795:intron | 30.0% | |
| TGAATTAGCCATGGAAATTA+GGG | - | chr8.3:63482674-63482693 | MS.gene001795:intron | 30.0% | |
| TTGAATTAGCCATGGAAATT+AGG | - | chr8.3:63482673-63482692 | MS.gene001795:intron | 30.0% | |
| ! | ACATTTTGAACAGCTTTTCA+AGG | - | chr8.3:63482370-63482389 | MS.gene001795:CDS | 30.0% |
| ! | TCATTTTGCACAACTTTTCA+TGG | - | chr8.3:63483405-63483424 | MS.gene001795:intron | 30.0% |
| ! | TTCATAAATGAAGGTGCATT+CGG | - | chr8.3:63483217-63483236 | MS.gene001795:intron | 30.0% |
| ! | TTTTATTAAACAGGTGACAC+TGG | - | chr8.3:63483279-63483298 | MS.gene001795:intron | 30.0% |
| AAAGCAATCATACGTTTCGT+TGG | - | chr8.3:63483137-63483156 | MS.gene001795:intron | 35.0% | |
| AACATGGAAGATAGTCTACT+TGG | + | chr8.3:63482926-63482945 | None:intergenic | 35.0% | |
| ATCATACGTTTCGTTGGATA+TGG | - | chr8.3:63483143-63483162 | MS.gene001795:intron | 35.0% | |
| GGTCCAAGTTATATGAAATC+AGG | + | chr8.3:63483101-63483120 | None:intergenic | 35.0% | |
| ! | TGTTGTGATCCTTGATTTTG+TGG | - | chr8.3:63483044-63483063 | MS.gene001795:intron | 35.0% |
| !! | GGCAATTTTAGCAAGAACTT+TGG | - | chr8.3:63482398-63482417 | MS.gene001795:CDS | 35.0% |
| !! | TTTTTGGTCCCTAATTTCCA+TGG | + | chr8.3:63482685-63482704 | None:intergenic | 35.0% |
| AAATCTGCACACAAGAGGAA+TGG | - | chr8.3:63483454-63483473 | MS.gene001795:intron | 40.0% | |
| AGACTATCTTCCATGTTCAG+TGG | - | chr8.3:63482929-63482948 | MS.gene001795:CDS | 40.0% | |
| ATAGAGAATTCCATCCATGG+AGG | + | chr8.3:63482342-63482361 | None:intergenic | 40.0% | |
| ATTAAACAGGTGACACTGGA+AGG | - | chr8.3:63483283-63483302 | MS.gene001795:intron | 40.0% | |
| CAAAATGAACCCTGCAGTAT+AGG | + | chr8.3:63483393-63483412 | None:intergenic | 40.0% | |
| CATAGCTTGAATGTGTGAGA+GGG | + | chr8.3:63483354-63483373 | None:intergenic | 40.0% | |
| GCAATAGAGAATTCCATCCA+TGG | + | chr8.3:63482345-63482364 | None:intergenic | 40.0% | |
| GTTTCTTCTGGATGATCTTC+AGG | + | chr8.3:63483535-63483554 | None:intergenic | 40.0% | |
| TACTCAAATCTGCACACAAG+AGG | - | chr8.3:63483449-63483468 | MS.gene001795:intron | 40.0% | |
| TCTACCACAAGACATGCTTT+AGG | - | chr8.3:63483311-63483330 | MS.gene001795:intron | 40.0% | |
| TTCTGACAAAGCAAATGAGC+TGG | - | chr8.3:63482566-63482585 | MS.gene001795:CDS | 40.0% | |
| ! | AACCTTTTCCAGTGGATAGA+CGG | + | chr8.3:63482987-63483006 | None:intergenic | 40.0% |
| ! | GCACAACTTTTCATGGAGAA+AGG | - | chr8.3:63483412-63483431 | MS.gene001795:intron | 40.0% |
| ! | TGTCGCGATTCTTGATTTTG+AGG | - | chr8.3:63483007-63483026 | MS.gene001795:intron | 40.0% |
| ! | TGTGTTACGACTTTTGGTCA+CGG | + | chr8.3:63483250-63483269 | None:intergenic | 40.0% |
| !! | GAACAGCTTTTCAAGGAATC+TGG | - | chr8.3:63482377-63482396 | MS.gene001795:CDS | 40.0% |
| AACCGTCTATCCACTGGAAA+AGG | - | chr8.3:63482982-63483001 | MS.gene001795:intron | 45.0% | |
| AGTACATACTCCTCCATGGA+TGG | - | chr8.3:63482329-63482348 | MS.gene001795:CDS | 45.0% | |
| CACACCTAAAGCATGTCTTG+TGG | + | chr8.3:63483318-63483337 | None:intergenic | 45.0% | |
| GCATAGCTTGAATGTGTGAG+AGG | + | chr8.3:63483355-63483374 | None:intergenic | 45.0% | |
| TCAAGCTATGCTGCTCTTGA+TGG | - | chr8.3:63483364-63483383 | MS.gene001795:intron | 45.0% | |
| ! | TGATGGTGTCCTATACTGCA+GGG | - | chr8.3:63483381-63483400 | MS.gene001795:intron | 45.0% |
| !! | CTCTAAAACCGTCTATCCAC+TGG | - | chr8.3:63482976-63482995 | MS.gene001795:intron | 45.0% |
| !! | TTGATGGTGTCCTATACTGC+AGG | - | chr8.3:63483380-63483399 | MS.gene001795:intron | 45.0% |
| !! | AAAATAAAAATAAAAAATGA+AGG | + | chr8.3:63482435-63482454 | None:intergenic | 5.0% |
| AGCAAGTCGCACATTTGTCC+AGG | + | chr8.3:63482958-63482977 | None:intergenic | 50.0% | |
| GCAAGTCGCACATTTGTCCA+GGG | + | chr8.3:63482957-63482976 | None:intergenic | 50.0% | |
| TTCCATGTTCAGTGGTACCC+TGG | - | chr8.3:63482937-63482956 | MS.gene001795:CDS | 50.0% | |
| ! | GCTTTAGGTGTGCTCATGCA+GGG | - | chr8.3:63483326-63483345 | MS.gene001795:intron | 50.0% |
| ! | GGTGCTTCTGCTGATTGTGA+AGG | + | chr8.3:63483514-63483533 | None:intergenic | 50.0% |
| ! | TCGCGACAAACCTTTTCCAG+TGG | + | chr8.3:63482995-63483014 | None:intergenic | 50.0% |
| ! | TGCTTTAGGTGTGCTCATGC+AGG | - | chr8.3:63483325-63483344 | MS.gene001795:intron | 50.0% |
| ! | GTCCAGGGTACCACTGAACA+TGG | + | chr8.3:63482942-63482961 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 63482326 | 63483561 | 63482326 | ID=MS.gene001795 |
| chr8.3 | mRNA | 63482326 | 63483561 | 63482326 | ID=MS.gene001795.t1;Parent=MS.gene001795 |
| chr8.3 | exon | 63483468 | 63483561 | 63483468 | ID=MS.gene001795.t1.exon1;Parent=MS.gene001795.t1 |
| chr8.3 | CDS | 63483468 | 63483561 | 63483468 | ID=cds.MS.gene001795.t1;Parent=MS.gene001795.t1 |
| chr8.3 | exon | 63483300 | 63483328 | 63483300 | ID=MS.gene001795.t1.exon2;Parent=MS.gene001795.t1 |
| chr8.3 | CDS | 63483300 | 63483328 | 63483300 | ID=cds.MS.gene001795.t1;Parent=MS.gene001795.t1 |
| chr8.3 | exon | 63482884 | 63482973 | 63482884 | ID=MS.gene001795.t1.exon3;Parent=MS.gene001795.t1 |
| chr8.3 | CDS | 63482884 | 63482973 | 63482884 | ID=cds.MS.gene001795.t1;Parent=MS.gene001795.t1 |
| chr8.3 | exon | 63482326 | 63482595 | 63482326 | ID=MS.gene001795.t1.exon4;Parent=MS.gene001795.t1 |
| chr8.3 | CDS | 63482326 | 63482595 | 63482326 | ID=cds.MS.gene001795.t1;Parent=MS.gene001795.t1 |
| Gene Sequence |
| Protein sequence |
