Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001934.t1 | XP_003622139.1 | 93.7 | 222 | 11 | 1 | 1 | 222 | 66 | 284 | 5.30E-99 | 370.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001934.t1 | Q9LSD5 | 58.9 | 209 | 63 | 6 | 1 | 203 | 94 | 285 | 1.5e-38 | 161.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001934.t1 | G7L283 | 93.7 | 222 | 11 | 1 | 1 | 222 | 66 | 284 | 3.8e-99 | 370.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene001934.t1 | TF | TCP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001934.t1 | MTR_7g028160 | 96.847 | 222 | 4 | 1 | 1 | 222 | 66 | 284 | 7.41e-155 | 431 |
| MS.gene001934.t1 | MTR_5g039600 | 57.292 | 96 | 24 | 3 | 1 | 79 | 83 | 178 | 3.45e-25 | 102 |
| MS.gene001934.t1 | MTR_2g006150 | 75.000 | 64 | 10 | 1 | 1 | 58 | 146 | 209 | 1.65e-24 | 100 |
| MS.gene001934.t1 | MTR_2g006150 | 75.000 | 64 | 10 | 1 | 1 | 58 | 146 | 209 | 1.67e-24 | 100 |
| MS.gene001934.t1 | MTR_8g033070 | 70.968 | 62 | 18 | 0 | 1 | 62 | 96 | 157 | 2.22e-24 | 99.0 |
| MS.gene001934.t1 | MTR_4g108370 | 89.362 | 47 | 5 | 0 | 1 | 47 | 113 | 159 | 8.43e-24 | 98.6 |
| MS.gene001934.t1 | MTR_1g101810 | 70.968 | 62 | 18 | 0 | 1 | 62 | 80 | 141 | 1.01e-23 | 97.1 |
| MS.gene001934.t1 | MTR_1g063870 | 85.106 | 47 | 7 | 0 | 1 | 47 | 65 | 111 | 1.53e-23 | 94.0 |
| MS.gene001934.t1 | MTR_1g101810 | 70.968 | 62 | 18 | 0 | 1 | 62 | 110 | 171 | 1.56e-23 | 97.1 |
| MS.gene001934.t1 | MTR_1g038650 | 62.162 | 74 | 28 | 0 | 1 | 74 | 48 | 121 | 4.06e-23 | 93.6 |
| MS.gene001934.t1 | MTR_1g114380 | 83.333 | 48 | 8 | 0 | 1 | 48 | 49 | 96 | 2.25e-22 | 92.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene001934.t1 | AT3G27010 | 56.061 | 198 | 64 | 7 | 1 | 192 | 94 | 274 | 2.46e-49 | 164 |
| MS.gene001934.t1 | AT3G27010 | 56.061 | 198 | 64 | 7 | 1 | 192 | 94 | 274 | 2.46e-49 | 164 |
| MS.gene001934.t1 | AT1G58100 | 75.000 | 64 | 10 | 1 | 1 | 58 | 76 | 139 | 2.61e-25 | 102 |
| MS.gene001934.t1 | AT5G41030 | 35.437 | 206 | 69 | 11 | 1 | 204 | 84 | 227 | 1.02e-24 | 98.2 |
| MS.gene001934.t1 | AT2G45680 | 64.706 | 68 | 24 | 0 | 1 | 68 | 91 | 158 | 4.52e-24 | 98.6 |
| MS.gene001934.t1 | AT3G47620 | 91.489 | 47 | 4 | 0 | 1 | 47 | 133 | 179 | 6.16e-24 | 99.4 |
| MS.gene001934.t1 | AT1G69690 | 85.417 | 48 | 7 | 0 | 1 | 48 | 68 | 115 | 6.23e-24 | 97.4 |
| MS.gene001934.t1 | AT1G35560 | 73.438 | 64 | 11 | 1 | 1 | 58 | 71 | 134 | 7.34e-24 | 97.8 |
| MS.gene001934.t1 | AT1G72010 | 87.234 | 47 | 6 | 0 | 1 | 47 | 79 | 125 | 1.91e-23 | 97.1 |
| MS.gene001934.t1 | AT5G51910 | 65.625 | 64 | 22 | 0 | 1 | 64 | 73 | 136 | 2.22e-23 | 95.5 |
| MS.gene001934.t1 | AT5G51910 | 65.625 | 64 | 22 | 0 | 1 | 64 | 73 | 136 | 2.22e-23 | 95.5 |
| MS.gene001934.t1 | AT5G08330 | 72.881 | 59 | 16 | 0 | 1 | 59 | 47 | 105 | 2.56e-23 | 94.4 |
| MS.gene001934.t1 | AT5G23280 | 83.333 | 48 | 8 | 0 | 1 | 48 | 55 | 102 | 1.35e-22 | 92.4 |
| MS.gene001934.t1 | AT2G37000 | 84.091 | 44 | 7 | 0 | 1 | 44 | 59 | 102 | 4.18e-19 | 82.0 |
| MS.gene001934.t1 | AT3G45150 | 58.140 | 43 | 18 | 0 | 1 | 43 | 33 | 75 | 1.04e-11 | 61.6 |
Find 55 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATGCCTGGTTTGGAATT+AGG | 0.117190 | 7.3:+72496713 | MS.gene001934:CDS |
| ATGTTAGTTCTACAAGTTTA+TGG | 0.160920 | 7.3:+72496495 | MS.gene001934:CDS |
| CAAGGTTCATCTTTGTCTTC+TGG | 0.222188 | 7.3:+72496395 | MS.gene001934:CDS |
| TATTATTATTCTGCTGATTC+TGG | 0.237820 | 7.3:-72496829 | None:intergenic |
| CATCATCAAGTTGGTGGATA+TGG | 0.242402 | 7.3:+72496521 | MS.gene001934:CDS |
| TATGCCAACAGGAACAAATT+TGG | 0.247795 | 7.3:+72496643 | MS.gene001934:CDS |
| AGTTGAATGATAGGAATAAT+TGG | 0.264011 | 7.3:+72496423 | MS.gene001934:CDS |
| AGATGCCTGGTTTGGAATTA+GGG | 0.264298 | 7.3:+72496714 | MS.gene001934:CDS |
| GATGGGCCTGATGGGCTTGA+TGG | 0.267687 | 7.3:-72496454 | None:intergenic |
| GCTTCAGCTTTAGCTTCTTC+TGG | 0.304919 | 7.3:+72496356 | MS.gene001934:CDS |
| CCTGTTGCAGCTATGATTGA+TGG | 0.306906 | 7.3:-72496317 | None:intergenic |
| GAACTGTGAGAATCATCTTT+AGG | 0.314551 | 7.3:-72496854 | None:intergenic |
| TTTCATCAATCATCATCTTC+TGG | 0.335762 | 7.3:+72496545 | MS.gene001934:CDS |
| TTTCAACTAACAAGAGAGTT+AGG | 0.353942 | 7.3:+72496245 | MS.gene001934:CDS |
| ATGGGCCTGATGGGCCTGAT+GGG | 0.381397 | 7.3:-72496462 | None:intergenic |
| GATGGGCCTGATGGGCCTGA+TGG | 0.383776 | 7.3:-72496463 | None:intergenic |
| CTGGTTTGCAGTTGAATGAT+AGG | 0.398808 | 7.3:+72496414 | MS.gene001934:CDS |
| ATGGGCCTGATGGGCTTGAT+GGG | 0.400129 | 7.3:-72496453 | None:intergenic |
| ATGCCAACAGGAACAAATTT+GGG | 0.405289 | 7.3:+72496644 | MS.gene001934:CDS |
| TCAAGCCCATCAGGCCCATC+AGG | 0.407369 | 7.3:+72496457 | MS.gene001934:CDS |
| TCAGACCCATCAAGCCCATC+AGG | 0.425492 | 7.3:+72496448 | MS.gene001934:CDS |
| ATCATAGCTGCAACAGGAAC+AGG | 0.443120 | 7.3:+72496323 | MS.gene001934:CDS |
| ACTCAGATTTATCAGCAGAT+TGG | 0.447988 | 7.3:+72496782 | MS.gene001934:CDS |
| TCTGGATTTGATATGCCAAC+AGG | 0.449142 | 7.3:+72496632 | MS.gene001934:CDS |
| ACTCCCAAATTTGTTCCTGT+TGG | 0.452759 | 7.3:-72496647 | None:intergenic |
| GTTGAATGATAGGAATAATT+GGG | 0.466051 | 7.3:+72496424 | MS.gene001934:CDS |
| AAATCAGCAGATGCCTGGTT+TGG | 0.470684 | 7.3:+72496706 | MS.gene001934:CDS |
| AACTAACATGATGACCTTGA+TGG | 0.471715 | 7.3:-72496481 | None:intergenic |
| ATGACCTTGATGGGCCTGAT+GGG | 0.473600 | 7.3:-72496471 | None:intergenic |
| CCATCAATCATAGCTGCAAC+AGG | 0.479395 | 7.3:+72496317 | MS.gene001934:CDS |
| TTGTCACAAGATGGACATAT+TGG | 0.480930 | 7.3:+72496737 | MS.gene001934:CDS |
| GAATTAGGGTTGTCACAAGA+TGG | 0.488895 | 7.3:+72496728 | MS.gene001934:CDS |
| ACTGTTGGTGAAAATAATAG+TGG | 0.505174 | 7.3:+72496584 | MS.gene001934:CDS |
| TGGCCTCATCATCAAGTTGG+TGG | 0.511204 | 7.3:+72496515 | MS.gene001934:CDS |
| GGTGCAAATCAGCAGATGCC+TGG | 0.519917 | 7.3:+72496701 | MS.gene001934:CDS |
| CAGATGGTGAAACAATTCAA+TGG | 0.529264 | 7.3:+72496276 | MS.gene001934:CDS |
| AGATTGGTCAAAATCAAACT+AGG | 0.530078 | 7.3:+72496798 | MS.gene001934:CDS |
| ATTCTTGCTGCACATAAAGC+TGG | 0.536084 | 7.3:-72496221 | None:intergenic |
| AACAGGAACAAATTTGGGAG+TGG | 0.545963 | 7.3:+72496649 | MS.gene001934:CDS |
| GGTTTAGTAGCTACTACTGT+TGG | 0.551441 | 7.3:+72496569 | MS.gene001934:CDS |
| CAGGCCCATCAGGCCCATCA+AGG | 0.557193 | 7.3:+72496467 | MS.gene001934:CDS |
| GATGACCTTGATGGGCCTGA+TGG | 0.557322 | 7.3:-72496472 | None:intergenic |
| ACTAACATGATGACCTTGAT+GGG | 0.557914 | 7.3:-72496480 | None:intergenic |
| CATCAATCATCATCTTCTGG+TGG | 0.564429 | 7.3:+72496548 | MS.gene001934:CDS |
| GGAACAAATTTGGGAGTGGG+AGG | 0.568972 | 7.3:+72496653 | MS.gene001934:CDS |
| GAAGAAGCTAAAGCTGAAGC+TGG | 0.576280 | 7.3:-72496353 | None:intergenic |
| GGTAATACTTTGACACCACA+AGG | 0.588387 | 7.3:+72496377 | MS.gene001934:CDS |
| ACAGGAACAAATTTGGGAGT+GGG | 0.594534 | 7.3:+72496650 | MS.gene001934:CDS |
| GATTGGTCAAAATCAAACTA+GGG | 0.595101 | 7.3:+72496799 | MS.gene001934:CDS |
| GAACAAATTTGGGAGTGGGA+GGG | 0.598189 | 7.3:+72496654 | MS.gene001934:CDS |
| TTATGGCCTCATCATCAAGT+TGG | 0.605767 | 7.3:+72496512 | MS.gene001934:CDS |
| GAGTTAGGTCATAAATCAGA+TGG | 0.610512 | 7.3:+72496260 | MS.gene001934:CDS |
| TATCCACCAACTTGATGATG+AGG | 0.610804 | 7.3:-72496518 | None:intergenic |
| GACAACCCTAATTCCAAACC+AGG | 0.628289 | 7.3:-72496719 | None:intergenic |
| GAAGACAAAGATGAACCTTG+TGG | 0.735014 | 7.3:-72496392 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATGAGTTTTACATCAATTTT+GGG | + | chr7.3:72496677-72496696 | MS.gene001934:CDS | 20.0% |
| !!! | GGAAATTATTTTCAGAAAAT+TGG | + | chr7.3:72496605-72496624 | MS.gene001934:CDS | 20.0% |
| ! | AGTTGAATGATAGGAATAAT+TGG | + | chr7.3:72496423-72496442 | MS.gene001934:CDS | 25.0% |
| ! | GTTGAATGATAGGAATAATT+GGG | + | chr7.3:72496424-72496443 | MS.gene001934:CDS | 25.0% |
| !! | GATGAGTTTTACATCAATTT+TGG | + | chr7.3:72496676-72496695 | MS.gene001934:CDS | 25.0% |
| !! | TGAGTTTTACATCAATTTTG+GGG | + | chr7.3:72496678-72496697 | MS.gene001934:CDS | 25.0% |
| !! | TTTCAGAAAATTGGATTTTC+TGG | + | chr7.3:72496614-72496633 | MS.gene001934:CDS | 25.0% |
| !!! | ATGTTAGTTCTACAAGTTTA+TGG | + | chr7.3:72496495-72496514 | MS.gene001934:CDS | 25.0% |
| !!! | TATTATTATTCTGCTGATTC+TGG | - | chr7.3:72496832-72496851 | None:intergenic | 25.0% |
| TTTCAACTAACAAGAGAGTT+AGG | + | chr7.3:72496245-72496264 | MS.gene001934:CDS | 30.0% | |
| TTTCATCAATCATCATCTTC+TGG | + | chr7.3:72496545-72496564 | MS.gene001934:CDS | 30.0% | |
| ! | ACTGTTGGTGAAAATAATAG+TGG | + | chr7.3:72496584-72496603 | MS.gene001934:CDS | 30.0% |
| ! | AGATTGGTCAAAATCAAACT+AGG | + | chr7.3:72496798-72496817 | MS.gene001934:CDS | 30.0% |
| ! | AGTTTTACATCAATTTTGGG+GGG | + | chr7.3:72496680-72496699 | MS.gene001934:CDS | 30.0% |
| ! | GAGTTTTACATCAATTTTGG+GGG | + | chr7.3:72496679-72496698 | MS.gene001934:CDS | 30.0% |
| ! | GATTGGTCAAAATCAAACTA+GGG | + | chr7.3:72496799-72496818 | MS.gene001934:CDS | 30.0% |
| AACTAACATGATGACCTTGA+TGG | - | chr7.3:72496484-72496503 | None:intergenic | 35.0% | |
| ACTAACATGATGACCTTGAT+GGG | - | chr7.3:72496483-72496502 | None:intergenic | 35.0% | |
| ACTCAGATTTATCAGCAGAT+TGG | + | chr7.3:72496782-72496801 | MS.gene001934:CDS | 35.0% | |
| ATGCCAACAGGAACAAATTT+GGG | + | chr7.3:72496644-72496663 | MS.gene001934:CDS | 35.0% | |
| CAGATGGTGAAACAATTCAA+TGG | + | chr7.3:72496276-72496295 | MS.gene001934:CDS | 35.0% | |
| GAACTGTGAGAATCATCTTT+AGG | - | chr7.3:72496857-72496876 | None:intergenic | 35.0% | |
| GAGTTAGGTCATAAATCAGA+TGG | + | chr7.3:72496260-72496279 | MS.gene001934:CDS | 35.0% | |
| TATGCCAACAGGAACAAATT+TGG | + | chr7.3:72496643-72496662 | MS.gene001934:CDS | 35.0% | |
| TTGTCACAAGATGGACATAT+TGG | + | chr7.3:72496737-72496756 | MS.gene001934:CDS | 35.0% | |
| AACAGGAACAAATTTGGGAG+TGG | + | chr7.3:72496649-72496668 | MS.gene001934:CDS | 40.0% | |
| ACAGGAACAAATTTGGGAGT+GGG | + | chr7.3:72496650-72496669 | MS.gene001934:CDS | 40.0% | |
| ATTCTTGCTGCACATAAAGC+TGG | - | chr7.3:72496224-72496243 | None:intergenic | 40.0% | |
| CAAGGTTCATCTTTGTCTTC+TGG | + | chr7.3:72496395-72496414 | MS.gene001934:CDS | 40.0% | |
| CATCAATCATCATCTTCTGG+TGG | + | chr7.3:72496548-72496567 | MS.gene001934:CDS | 40.0% | |
| GAAGACAAAGATGAACCTTG+TGG | - | chr7.3:72496395-72496414 | None:intergenic | 40.0% | |
| GAATTAGGGTTGTCACAAGA+TGG | + | chr7.3:72496728-72496747 | MS.gene001934:CDS | 40.0% | |
| GGTAATACTTTGACACCACA+AGG | + | chr7.3:72496377-72496396 | MS.gene001934:CDS | 40.0% | |
| GGTTTAGTAGCTACTACTGT+TGG | + | chr7.3:72496569-72496588 | MS.gene001934:CDS | 40.0% | |
| TCTGGATTTGATATGCCAAC+AGG | + | chr7.3:72496632-72496651 | MS.gene001934:CDS | 40.0% | |
| TTATGGCCTCATCATCAAGT+TGG | + | chr7.3:72496512-72496531 | MS.gene001934:CDS | 40.0% | |
| ! | ACTCCCAAATTTGTTCCTGT+TGG | - | chr7.3:72496650-72496669 | None:intergenic | 40.0% |
| ! | CTGGTTTGCAGTTGAATGAT+AGG | + | chr7.3:72496414-72496433 | MS.gene001934:CDS | 40.0% |
| !! | AGATGCCTGGTTTGGAATTA+GGG | + | chr7.3:72496714-72496733 | MS.gene001934:CDS | 40.0% |
| !! | CATCATCAAGTTGGTGGATA+TGG | + | chr7.3:72496521-72496540 | MS.gene001934:CDS | 40.0% |
| !! | TATCCACCAACTTGATGATG+AGG | - | chr7.3:72496521-72496540 | None:intergenic | 40.0% |
| AAATCAGCAGATGCCTGGTT+TGG | + | chr7.3:72496706-72496725 | MS.gene001934:CDS | 45.0% | |
| ATCATAGCTGCAACAGGAAC+AGG | + | chr7.3:72496323-72496342 | MS.gene001934:CDS | 45.0% | |
| CCATCAATCATAGCTGCAAC+AGG | + | chr7.3:72496317-72496336 | MS.gene001934:CDS | 45.0% | |
| CCTGTTGCAGCTATGATTGA+TGG | - | chr7.3:72496320-72496339 | None:intergenic | 45.0% | |
| GAACAAATTTGGGAGTGGGA+GGG | + | chr7.3:72496654-72496673 | MS.gene001934:CDS | 45.0% | |
| GAAGAAGCTAAAGCTGAAGC+TGG | - | chr7.3:72496356-72496375 | None:intergenic | 45.0% | |
| GACAACCCTAATTCCAAACC+AGG | - | chr7.3:72496722-72496741 | None:intergenic | 45.0% | |
| ! | GCTTCAGCTTTAGCTTCTTC+TGG | + | chr7.3:72496356-72496375 | MS.gene001934:CDS | 45.0% |
| !! | CAGATGCCTGGTTTGGAATT+AGG | + | chr7.3:72496713-72496732 | MS.gene001934:CDS | 45.0% |
| GGAACAAATTTGGGAGTGGG+AGG | + | chr7.3:72496653-72496672 | MS.gene001934:CDS | 50.0% | |
| ! | ATGACCTTGATGGGCCTGAT+GGG | - | chr7.3:72496474-72496493 | None:intergenic | 50.0% |
| !! | TGGCCTCATCATCAAGTTGG+TGG | + | chr7.3:72496515-72496534 | MS.gene001934:CDS | 50.0% |
| GGTGCAAATCAGCAGATGCC+TGG | + | chr7.3:72496701-72496720 | MS.gene001934:CDS | 55.0% | |
| TCAGACCCATCAAGCCCATC+AGG | + | chr7.3:72496448-72496467 | MS.gene001934:CDS | 55.0% | |
| ! | GATGACCTTGATGGGCCTGA+TGG | - | chr7.3:72496475-72496494 | None:intergenic | 55.0% |
| !! | ATGGGCCTGATGGGCTTGAT+GGG | - | chr7.3:72496456-72496475 | None:intergenic | 55.0% |
| TCAAGCCCATCAGGCCCATC+AGG | + | chr7.3:72496457-72496476 | MS.gene001934:CDS | 60.0% | |
| ! | ATGGGCCTGATGGGCCTGAT+GGG | - | chr7.3:72496465-72496484 | None:intergenic | 60.0% |
| !! | GATGGGCCTGATGGGCTTGA+TGG | - | chr7.3:72496457-72496476 | None:intergenic | 60.0% |
| CAGGCCCATCAGGCCCATCA+AGG | + | chr7.3:72496467-72496486 | MS.gene001934:CDS | 65.0% | |
| ! | GATGGGCCTGATGGGCCTGA+TGG | - | chr7.3:72496466-72496485 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 72496218 | 72496886 | 72496218 | ID=MS.gene001934 |
| chr7.3 | mRNA | 72496218 | 72496886 | 72496218 | ID=MS.gene001934.t1;Parent=MS.gene001934 |
| chr7.3 | exon | 72496218 | 72496886 | 72496218 | ID=MS.gene001934.t1.exon1;Parent=MS.gene001934.t1 |
| chr7.3 | CDS | 72496218 | 72496886 | 72496218 | ID=cds.MS.gene001934.t1;Parent=MS.gene001934.t1 |
| Gene Sequence |
| Protein sequence |
