AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00194

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00194.t1 MTR_0402s0040 58.222 450 106 8 33 438 1 412 2.61e-152 441
MS.gene00194.t1 MTR_6g017200 86.861 137 18 0 1 137 1 137 1.06e-86 261
MS.gene00194.t1 MTR_2g070590 87.234 94 12 0 44 137 3 96 1.93e-54 177
MS.gene00194.t1 MTR_1g057920 74.528 106 27 0 32 137 8 113 3.93e-52 171
MS.gene00194.t1 MTR_0291s0060 78.313 83 18 0 26 108 3 85 1.25e-37 132
MS.gene00194.t1 MTR_5g015480 61.538 104 36 1 34 137 36 135 8.44e-33 125
MS.gene00194.t1 MTR_0137s0040 59.140 93 38 0 34 126 12 104 1.55e-31 117
MS.gene00194.t1 MTR_7g113010 58.491 106 30 1 32 137 431 522 1.37e-29 121
MS.gene00194.t1 MTR_1g091013 51.282 78 27 1 49 126 3 69 1.91e-15 71.2
MS.gene00194.t1 MTR_5g045210 41.573 89 52 0 328 416 20 108 4.35e-15 73.9
MS.gene00194.t1 MTR_4g102720 38.776 98 48 2 58 144 7 103 7.01e-15 73.9
MS.gene00194.t1 MTR_2g098920 43.750 80 44 1 58 137 80 158 3.69e-12 66.6
MS.gene00194.t1 MTR_4g067290 43.750 80 44 1 58 137 56 134 3.73e-12 66.2
MS.gene00194.t1 MTR_4g067290 43.750 80 44 1 58 137 56 134 4.33e-12 66.2
MS.gene00194.t1 MTR_4g067290 43.750 80 44 1 58 137 74 152 5.55e-12 66.2
MS.gene00194.t1 MTR_4g067290 43.750 80 44 1 58 137 76 154 5.57e-12 66.2
MS.gene00194.t1 MTR_4g067290 43.750 80 44 1 58 137 76 154 5.59e-12 66.2
MS.gene00194.t1 MTR_4g067290 43.750 80 44 1 58 137 116 194 7.59e-12 66.2
MS.gene00194.t1 MTR_0337s0060 26.437 174 97 4 187 356 21 167 9.80e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00194.t1 AT3G22170 30.916 262 158 6 145 396 409 657 8.78e-33 132
MS.gene00194.t1 AT3G22170 30.916 262 158 6 145 396 409 657 8.78e-33 132
MS.gene00194.t1 AT4G38180 30.466 279 188 4 137 410 416 693 9.58e-33 131
MS.gene00194.t1 AT4G38180 36.667 120 71 2 33 147 46 165 7.55e-12 68.2
MS.gene00194.t1 AT2G27110 31.786 280 177 8 137 409 218 490 1.71e-31 127
MS.gene00194.t1 AT2G27110 31.786 280 177 8 137 409 363 635 1.80e-31 128
MS.gene00194.t1 AT2G27110 31.786 280 177 8 137 409 363 635 1.80e-31 128
MS.gene00194.t1 AT4G15090 27.273 275 190 3 143 413 398 666 7.97e-28 117
MS.gene00194.t1 AT4G15090 27.273 275 190 3 143 413 398 666 7.97e-28 117
MS.gene00194.t1 AT4G15090 27.273 275 190 3 143 413 398 666 7.97e-28 117
MS.gene00194.t1 AT4G15090 27.273 275 190 3 143 413 398 666 7.97e-28 117
MS.gene00194.t1 AT4G15090 27.273 275 190 3 143 413 398 666 7.97e-28 117
MS.gene00194.t1 AT4G38170 27.650 217 151 3 141 352 185 400 2.29e-26 112
MS.gene00194.t1 AT1G76320 28.846 260 169 8 143 396 335 584 2.45e-26 112
MS.gene00194.t1 AT1G76320 28.846 260 169 8 143 396 335 584 2.69e-26 112
MS.gene00194.t1 AT1G76320 28.846 260 169 8 143 396 335 584 2.69e-26 112
MS.gene00194.t1 AT1G76320 28.846 260 169 8 143 396 335 584 3.02e-26 112
MS.gene00194.t1 AT5G18960 28.253 269 181 6 145 408 521 782 1.38e-25 110
MS.gene00194.t1 AT5G18960 28.253 269 181 6 145 408 521 782 1.38e-25 110
MS.gene00194.t1 AT3G06250 28.253 269 181 6 145 408 497 758 1.47e-25 110
MS.gene00194.t1 AT3G06250 28.253 269 181 6 145 408 497 758 1.47e-25 110
MS.gene00194.t1 AT3G06250 28.253 269 181 6 145 408 497 758 1.47e-25 110
MS.gene00194.t1 AT4G19990 28.309 272 176 4 145 406 348 610 7.77e-24 105
MS.gene00194.t1 AT4G19990 28.309 272 176 4 145 406 348 610 7.77e-24 105
MS.gene00194.t1 AT4G19990 28.309 272 176 4 145 406 348 610 7.96e-24 105
MS.gene00194.t1 AT4G19990 28.309 272 176 4 145 406 362 624 8.08e-24 105
MS.gene00194.t1 AT4G19990 28.309 272 176 4 145 406 291 553 8.10e-24 104
MS.gene00194.t1 AT4G19990 28.309 272 176 4 145 406 348 610 8.89e-24 104
MS.gene00194.t1 AT4G19990 28.309 272 176 4 145 406 348 610 8.89e-24 104
MS.gene00194.t1 AT2G32250 25.843 267 188 4 146 408 345 605 3.46e-21 97.1
MS.gene00194.t1 AT2G32250 25.843 267 188 4 146 408 345 605 3.46e-21 97.1
MS.gene00194.t1 AT2G32250 25.843 267 188 4 146 408 345 605 3.46e-21 97.1
MS.gene00194.t1 AT2G32250 25.843 267 188 4 146 408 345 605 3.69e-21 97.1
MS.gene00194.t1 AT2G32250 25.843 267 188 4 146 408 345 605 3.82e-21 97.1
MS.gene00194.t1 AT2G32250 25.843 267 188 4 146 408 345 605 3.82e-21 97.1
MS.gene00194.t1 AT2G32250 25.468 267 189 4 146 408 304 564 4.01e-21 96.7
MS.gene00194.t1 AT2G32250 25.468 267 189 4 146 408 304 564 4.12e-21 96.7
MS.gene00194.t1 AT1G52520 26.606 218 146 2 145 349 420 636 2.52e-18 88.2
MS.gene00194.t1 AT1G10240 26.596 282 183 7 145 413 402 672 1.64e-16 82.4
MS.gene00194.t1 AT1G10240 26.596 282 183 7 145 413 402 672 1.64e-16 82.4
MS.gene00194.t1 AT5G28530 25.591 254 171 5 123 371 401 641 1.14e-15 79.7
MS.gene00194.t1 AT5G28530 25.197 254 172 4 123 371 338 578 1.47e-15 79.3
MS.gene00194.t1 AT5G28530 25.197 254 168 5 123 371 401 637 2.22e-14 75.9
MS.gene00194.t1 AT5G28530 25.197 254 168 5 123 371 401 637 2.22e-14 75.9
MS.gene00194.t1 AT5G28530 25.197 254 168 5 123 371 338 574 2.76e-14 75.5
MS.gene00194.t1 AT3G59470 34.722 144 81 3 25 158 27 167 1.11e-12 68.2
MS.gene00194.t1 AT3G59470 34.722 144 81 3 25 158 27 167 1.11e-12 68.2
MS.gene00194.t1 AT3G59470 34.722 144 81 3 25 158 39 179 1.56e-12 67.8
MS.gene00194.t1 AT1G80010 22.768 224 149 5 145 351 445 661 7.25e-12 68.2

Find 87 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGCTTAAGTATATAGGTTTC+AGG 0.211352 2.1:-58108582 None:intergenic
TTTGAAGTCTGATTTAAATT+TGG 0.211881 2.1:+58108182 MS.gene00194:CDS
TTCAGATTGTGTCAATAAAT+TGG 0.215486 2.1:+58106877 MS.gene00194:intron
TTCATCATTTGCAATTGATA+TGG 0.235636 2.1:-58108859 None:intergenic
TTTGGTATTCGTCGAGAATA+TGG 0.287007 2.1:+58107034 MS.gene00194:CDS
AAGTATGTGGATGGAGAATC+AGG 0.314708 2.1:+58108819 MS.gene00194:CDS
TCTGCTCTAGGCTCCCCTTT+TGG 0.322934 2.1:-58107145 None:intergenic
TGTCCATCGCTTAAGTATAT+AGG 0.330247 2.1:-58108589 None:intergenic
AATGAGTATAGTAAAAGAAT+TGG 0.336174 2.1:+58107010 MS.gene00194:CDS
ATGGTCGGAGACGGCGGCGG+CGG 0.340105 2.1:-58106381 None:intergenic
TGGAACTCCTCAAATATTCT+AGG 0.341058 2.1:-58108348 None:intergenic
CATTGTACCGCTTCATGAAA+TGG 0.342244 2.1:-58108677 None:intergenic
ATGAGTTTCTGAAGCTTTGT+TGG 0.343120 2.1:-58108727 None:intergenic
ACTTGTTGAGCATAATATAT+TGG 0.354013 2.1:+58108029 MS.gene00194:CDS
GGAAATAAAAGCAGAGTTGA+TGG 0.361988 2.1:+58107055 MS.gene00194:CDS
GATATTCTTCATATTCCTCT+TGG 0.370740 2.1:-58108368 None:intergenic
AGAAATTAGAATGGGATGTT+TGG 0.393590 2.1:+58107165 MS.gene00194:CDS
TATAGTAAAAGAATTGGATT+TGG 0.393932 2.1:+58107016 MS.gene00194:CDS
TGCGTAAAAGATTTGAAAGA+AGG 0.394307 2.1:+58108399 MS.gene00194:CDS
TTACACTCCTAGAATATTTG+AGG 0.404149 2.1:+58108341 MS.gene00194:CDS
GCTGGACTCTTCATAAACTT+TGG 0.408472 2.1:-58108769 None:intergenic
AATTTAAATCAGACTTCAAA+TGG 0.428063 2.1:-58108179 None:intergenic
TGGTCGGAGACGGCGGCGGC+GGG 0.433188 2.1:-58106380 None:intergenic
GCATACAATTTATCAATTGA+AGG 0.436807 2.1:+58108065 MS.gene00194:CDS
ATTGTACCGCTTCATGAAAT+GGG 0.445421 2.1:-58108676 None:intergenic
AACAACAATGGCAGCGGCGC+AGG 0.452252 2.1:-58106405 None:intergenic
GACAAAAGACGTCAACCAAA+AGG 0.461065 2.1:+58107130 MS.gene00194:CDS
TAGTGTTGGTAGCAGGATGA+TGG 0.465525 2.1:-58106352 None:intergenic
CCAACGGAGAGGATAGTTAT+GGG 0.468436 2.1:+58108450 MS.gene00194:CDS
GGTTCGTTAGTGTTGGTAGC+AGG 0.468658 2.1:-58106359 None:intergenic
CCCATAACTATCCTCTCCGT+TGG 0.473881 2.1:-58108450 None:intergenic
TCCAACGGAGAGGATAGTTA+TGG 0.475548 2.1:+58108449 MS.gene00194:CDS
GCTGCCATTGTTGTTGATCA+AGG 0.480338 2.1:+58106413 MS.gene00194:CDS
GGTTGGGAAGTGATCAAGAT+TGG 0.482271 2.1:+58108622 MS.gene00194:CDS
CAAAATAATTGATGATATGT+TGG 0.486221 2.1:+58108791 MS.gene00194:CDS
TTGGTTAACTTAACCATTCG+AGG 0.487402 2.1:-58108708 None:intergenic
CCCATTCTAATTTCTGCTCT+AGG 0.487681 2.1:-58107157 None:intergenic
AGTGTTGGTAGCAGGATGAT+GGG 0.490017 2.1:-58106351 None:intergenic
ACAAAAGACGTCAACCAAAA+GGG 0.496414 2.1:+58107131 MS.gene00194:CDS
GATAGTTATGGGAAATGCTA+TGG 0.498511 2.1:+58108461 MS.gene00194:CDS
TGCCAACAATGTACCTCACT+TGG 0.498928 2.1:+58106946 MS.gene00194:CDS
TTCCAAGTGAGGTACATTGT+TGG 0.502644 2.1:-58106948 None:intergenic
GGCAGCGGCGCAGGGATGGT+CGG 0.512257 2.1:-58106396 None:intergenic
TGGACAAGAAATGCAAGGTT+GGG 0.513978 2.1:+58108606 MS.gene00194:CDS
ATGGACAAGAAATGCAAGGT+TGG 0.517510 2.1:+58108605 MS.gene00194:CDS
GGGATGGTCGGAGACGGCGG+CGG 0.517961 2.1:-58106384 None:intergenic
GGAAATGAAGTTTGATTCTG+AGG 0.525153 2.1:+58106967 MS.gene00194:CDS
GTTGAGCATAATATATTGGA+AGG 0.525933 2.1:+58108033 MS.gene00194:CDS
CAATGGCAGCGGCGCAGGGA+TGG 0.526019 2.1:-58106400 None:intergenic
GCCTAGAGCAGAAATTAGAA+TGG 0.527171 2.1:+58107156 MS.gene00194:CDS
GGCGCAGGGATGGTCGGAGA+CGG 0.533543 2.1:-58106390 None:intergenic
AATGGGCATGGACATATGTC+CGG 0.534093 2.1:+58108091 MS.gene00194:CDS
GGCGGCGGGTTCGTTAGTGT+TGG 0.541693 2.1:-58106366 None:intergenic
GAAGTTTGATTCTGAGGCAG+CGG 0.545649 2.1:+58106973 MS.gene00194:CDS
GCCTAGATTGAAAATGAAGA+AGG 0.546195 2.1:+58108290 MS.gene00194:CDS
GAAGTGATCAAGATTGGAAA+GGG 0.552959 2.1:+58108628 MS.gene00194:CDS
GTCCGGAAAACATTCACTGC+AGG 0.554763 2.1:+58108108 MS.gene00194:CDS
GCAGGGATGGTCGGAGACGG+CGG 0.559162 2.1:-58106387 None:intergenic
CCTAGAGCAGAAATTAGAAT+GGG 0.568736 2.1:+58107157 MS.gene00194:CDS
ACAACAATGGCAGCGGCGCA+GGG 0.575609 2.1:-58106404 None:intergenic
CAAAATGTGCAACACGGACA+AGG 0.581561 2.1:+58106917 MS.gene00194:CDS
GTGTTGGTAGCAGGATGATG+GGG 0.582430 2.1:-58106350 None:intergenic
GGAAGTGATCAAGATTGGAA+AGG 0.584639 2.1:+58108627 MS.gene00194:CDS
ATAATATATTGGAAGGGTCG+TGG 0.588775 2.1:+58108040 MS.gene00194:CDS
CAATGAGTTATGTCCTCGAA+TGG 0.589590 2.1:+58108695 MS.gene00194:CDS
AAACCTATATACTTAAGCGA+TGG 0.590891 2.1:+58108586 MS.gene00194:CDS
GATAGATGTACACGAAGATG+AGG 0.599215 2.1:+58107978 MS.gene00194:CDS
TCAAACTTCATTTCCAAGTG+AGG 0.602359 2.1:-58106959 None:intergenic
CAAAAGACGTCAACCAAAAG+GGG 0.607006 2.1:+58107132 MS.gene00194:CDS
TAAAAGCCCATTTCATGAAG+CGG 0.608976 2.1:+58108670 MS.gene00194:CDS
TGTCGTAAATTTGAGACACA+TGG 0.610330 2.1:+58108504 MS.gene00194:CDS
AAGATTCACATGCTACAAAG+AGG 0.619398 2.1:+58107093 MS.gene00194:CDS
TTGAGCATAATATATTGGAA+GGG 0.622690 2.1:+58108034 MS.gene00194:CDS
TTGATCAACAACAATGGCAG+CGG 0.627743 2.1:-58106411 None:intergenic
GTGATCAAGATTGGAAAGGG+AGG 0.630069 2.1:+58108631 MS.gene00194:CDS
AGTATGTGGATGGAGAATCA+GGG 0.630721 2.1:+58108820 MS.gene00194:CDS
TGCTACAAAGAGGGTGTACG+AGG 0.638548 2.1:+58107103 MS.gene00194:CDS
AGCGATGGACAAGAAATGCA+AGG 0.640628 2.1:+58108601 MS.gene00194:CDS
GTATGTGGATGGAGAATCAG+GGG 0.652149 2.1:+58108821 MS.gene00194:CDS
AGATTCACATGCTACAAAGA+GGG 0.654496 2.1:+58107094 MS.gene00194:CDS
ATAGATGTACACGAAGATGA+GGG 0.654743 2.1:+58107979 MS.gene00194:CDS
CACAAACTATGATAATCCAA+CGG 0.657053 2.1:+58108434 MS.gene00194:CDS
CATGAACAAAATGTGCAACA+CGG 0.658217 2.1:+58106911 MS.gene00194:CDS
ATTACCTTGATCAACAACAA+TGG 0.673823 2.1:-58106417 None:intergenic
AATATTTGAGGAGTTCCAAG+AGG 0.693235 2.1:+58108353 MS.gene00194:CDS
ACTATGATAATCCAACGGAG+AGG 0.733483 2.1:+58108439 MS.gene00194:CDS
TATGTGGATGGAGAATCAGG+GGG 0.769238 2.1:+58108822 MS.gene00194:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTACTATTAAAAAAACATT+GGG - chr2.1:58106620-58106639 None:intergenic 10.0%
!! TATTACTATTAAAAAAACAT+TGG - chr2.1:58106621-58106640 None:intergenic 10.0%
!! AAAACCAAAATTTACAATTA+AGG - chr2.1:58106857-58106876 None:intergenic 15.0%
!! TTACTATTAAAAAAACATTG+GGG - chr2.1:58106619-58106638 None:intergenic 15.0%
!!! AGCAAATAACAAATATATTT+TGG + chr2.1:58107563-58107582 MS.gene00194:intron 15.0%
!!! GCAAATAACAAATATATTTT+GGG + chr2.1:58107564-58107583 MS.gene00194:intron 15.0%
!!! TATACATATATATAGCTTTA+TGG + chr2.1:58106555-58106574 MS.gene00194:intron 15.0%
!! AATGAGTATAGTAAAAGAAT+TGG + chr2.1:58107010-58107029 MS.gene00194:CDS 20.0%
!! AATTTAAATCAGACTTCAAA+TGG - chr2.1:58108182-58108201 None:intergenic 20.0%
!! CACAAATGATAAATGATTAT+GGG + chr2.1:58107593-58107612 MS.gene00194:intron 20.0%
!! TACTATTAAAAAAACATTGG+GGG - chr2.1:58106618-58106637 None:intergenic 20.0%
!! TATAGTAAAAGAATTGGATT+TGG + chr2.1:58107016-58107035 MS.gene00194:CDS 20.0%
!! TTATCAATTGAAGGAAAAAT+GGG + chr2.1:58108074-58108093 MS.gene00194:CDS 20.0%
!! TTTATCAATTGAAGGAAAAA+TGG + chr2.1:58108073-58108092 MS.gene00194:CDS 20.0%
!!! AATAACAAATATATTTTGGG+CGG + chr2.1:58107567-58107586 MS.gene00194:intron 20.0%
!!! AATAGTAATAGATAGCTTTA+TGG + chr2.1:58106631-58106650 MS.gene00194:intron 20.0%
!!! ATAAATGATTATGGGTATTT+TGG + chr2.1:58107601-58107620 MS.gene00194:intron 20.0%
!!! ATATATTCATGCAAATCTTT+TGG - chr2.1:58107364-58107383 None:intergenic 20.0%
!!! CAAAATAATTGATGATATGT+TGG + chr2.1:58108791-58108810 MS.gene00194:CDS 20.0%
!!! TTGATGAAAAATTAGTTTCT+AGG + chr2.1:58106503-58106522 MS.gene00194:intron 20.0%
!!! TTTGAAGTCTGATTTAAATT+TGG + chr2.1:58108182-58108201 MS.gene00194:CDS 20.0%
! AACATCATGTAATAAAGAGA+CGG - chr2.1:58106472-58106491 None:intergenic 25.0%
! ATATAAGACAGAATGCTTTA+CGG + chr2.1:58107899-58107918 MS.gene00194:intron 25.0%
! GCACAAATGATAAATGATTA+TGG + chr2.1:58107592-58107611 MS.gene00194:intron 25.0%
! GCATACAATTTATCAATTGA+AGG + chr2.1:58108065-58108084 MS.gene00194:CDS 25.0%
! GGCAAATAATAAAATAGAGA+AGG - chr2.1:58106451-58106470 None:intergenic 25.0%
! GTTCTATTATGACTTTCAAA+TGG + chr2.1:58107531-58107550 MS.gene00194:intron 25.0%
! TATCAAAAGAGTTCACAATT+CGG + chr2.1:58107937-58107956 MS.gene00194:intron 25.0%
! TTCAGATTGTGTCAATAAAT+TGG + chr2.1:58106877-58106896 MS.gene00194:intron 25.0%
! TTCATCATTTGCAATTGATA+TGG - chr2.1:58108862-58108881 None:intergenic 25.0%
! TTGAGCATAATATATTGGAA+GGG + chr2.1:58108034-58108053 MS.gene00194:CDS 25.0%
! TTGTTGGACTAAATAATCAT+AGG + chr2.1:58107686-58107705 MS.gene00194:intron 25.0%
!! ACTTGTTGAGCATAATATAT+TGG + chr2.1:58108029-58108048 MS.gene00194:CDS 25.0%
!! GTTATTTGAAACATTTCTCA+AGG + chr2.1:58107768-58107787 MS.gene00194:intron 25.0%
!!! CATATCATCAATTATTTTGC+TGG - chr2.1:58108790-58108809 None:intergenic 25.0%
AAACCTATATACTTAAGCGA+TGG + chr2.1:58108586-58108605 MS.gene00194:CDS 30.0%
ACCACATGCAAAACAAATAA+GGG + chr2.1:58107643-58107662 MS.gene00194:intron 30.0%
AGAAATTAGAATGGGATGTT+TGG + chr2.1:58107165-58107184 MS.gene00194:CDS 30.0%
ATTACCTTGATCAACAACAA+TGG - chr2.1:58106420-58106439 None:intergenic 30.0%
CAAATAACCACTTGAAAGAA+GGG - chr2.1:58107756-58107775 None:intergenic 30.0%
CACAAACTATGATAATCCAA+CGG + chr2.1:58108434-58108453 MS.gene00194:CDS 30.0%
GATATTCTTCATATTCCTCT+TGG - chr2.1:58108371-58108390 None:intergenic 30.0%
GTTGAGCATAATATATTGGA+AGG + chr2.1:58108033-58108052 MS.gene00194:CDS 30.0%
TCAAATAACCACTTGAAAGA+AGG - chr2.1:58107757-58107776 None:intergenic 30.0%
TGAAAGTCATAATAGAACGA+GGG - chr2.1:58107529-58107548 None:intergenic 30.0%
TGCGTAAAAGATTTGAAAGA+AGG + chr2.1:58108399-58108418 MS.gene00194:CDS 30.0%
TTAAAAAAACATTGGGGGAA+AGG - chr2.1:58106613-58106632 None:intergenic 30.0%
TTACACTCCTAGAATATTTG+AGG + chr2.1:58108341-58108360 MS.gene00194:CDS 30.0%
TTGAAAGTCATAATAGAACG+AGG - chr2.1:58107530-58107549 None:intergenic 30.0%
! AAAGCCAAAAACCATTTTGA+CGG + chr2.1:58107804-58107823 MS.gene00194:intron 30.0%
! ATGTCTGAAATCATCACTTA+TGG + chr2.1:58106828-58106847 MS.gene00194:intron 30.0%
! GTGAACTCTTTTGATATAAG+TGG - chr2.1:58107933-58107952 None:intergenic 30.0%
!! GTGGCCTTAATTGTAAATTT+TGG + chr2.1:58106850-58106869 MS.gene00194:intron 30.0%
AAACAATCCCTTCTTTCAAG+TGG + chr2.1:58107746-58107765 MS.gene00194:intron 35.0%
AAAGAGTTCACAATTCGGTT+TGG + chr2.1:58107942-58107961 MS.gene00194:intron 35.0%
AAGATTCACATGCTACAAAG+AGG + chr2.1:58107093-58107112 MS.gene00194:CDS 35.0%
AATATTTGAGGAGTTCCAAG+AGG + chr2.1:58108353-58108372 MS.gene00194:CDS 35.0%
AATTGAAGGAAAAATGGGCA+TGG + chr2.1:58108079-58108098 MS.gene00194:CDS 35.0%
ACAAAAGACGTCAACCAAAA+GGG + chr2.1:58107131-58107150 MS.gene00194:CDS 35.0%
ACAAGCATCACAAATTGTCT+TGG + chr2.1:58107318-58107337 MS.gene00194:intron 35.0%
AGATTCACATGCTACAAAGA+GGG + chr2.1:58107094-58107113 MS.gene00194:CDS 35.0%
AGGAAACAGTCACTAAAGTA+TGG + chr2.1:58107457-58107476 MS.gene00194:intron 35.0%
ATAATATATTGGAAGGGTCG+TGG + chr2.1:58108040-58108059 MS.gene00194:CDS 35.0%
ATAGATGTACACGAAGATGA+GGG + chr2.1:58107979-58107998 MS.gene00194:CDS 35.0%
ATTGTACCGCTTCATGAAAT+GGG - chr2.1:58108679-58108698 None:intergenic 35.0%
CAAGCATCACAAATTGTCTT+GGG + chr2.1:58107319-58107338 MS.gene00194:intron 35.0%
CACAATCCATGAAATGTTTC+TGG - chr2.1:58107871-58107890 None:intergenic 35.0%
CACCACATGCAAAACAAATA+AGG + chr2.1:58107642-58107661 MS.gene00194:intron 35.0%
CATGAACAAAATGTGCAACA+CGG + chr2.1:58106911-58106930 MS.gene00194:CDS 35.0%
CCTAGAGCAGAAATTAGAAT+GGG + chr2.1:58107157-58107176 MS.gene00194:CDS 35.0%
CGAATTACCTTGAAACCAAA+AGG + chr2.1:58107437-58107456 MS.gene00194:intron 35.0%
CGCTTAAGTATATAGGTTTC+AGG - chr2.1:58108585-58108604 None:intergenic 35.0%
GAAACATTTCTCAAGGCAAT+GGG + chr2.1:58107775-58107794 MS.gene00194:intron 35.0%
GAAGTGATCAAGATTGGAAA+GGG + chr2.1:58108628-58108647 MS.gene00194:CDS 35.0%
GAATATATATCCAAGCAAGC+TGG + chr2.1:58107376-58107395 MS.gene00194:intron 35.0%
GATAGTTATGGGAAATGCTA+TGG + chr2.1:58108461-58108480 MS.gene00194:CDS 35.0%
GCCTAGATTGAAAATGAAGA+AGG + chr2.1:58108290-58108309 MS.gene00194:CDS 35.0%
GGAAATAAAAGCAGAGTTGA+TGG + chr2.1:58107055-58107074 MS.gene00194:CDS 35.0%
GGAAATGAAGTTTGATTCTG+AGG + chr2.1:58106967-58106986 MS.gene00194:CDS 35.0%
GTTATGCCAGAAACATTTCA+TGG + chr2.1:58107862-58107881 MS.gene00194:intron 35.0%
TAAAAGCCCATTTCATGAAG+CGG + chr2.1:58108670-58108689 MS.gene00194:CDS 35.0%
TCAAACTTCATTTCCAAGTG+AGG - chr2.1:58106962-58106981 None:intergenic 35.0%
TGAAACATTTCTCAAGGCAA+TGG + chr2.1:58107774-58107793 MS.gene00194:intron 35.0%
TGGAACTCCTCAAATATTCT+AGG - chr2.1:58108351-58108370 None:intergenic 35.0%
TGGTTTCAAGGTAATTCGTA+AGG - chr2.1:58107435-58107454 None:intergenic 35.0%
TGTCCATCGCTTAAGTATAT+AGG - chr2.1:58108592-58108611 None:intergenic 35.0%
TTGGTTAACTTAACCATTCG+AGG - chr2.1:58108711-58108730 None:intergenic 35.0%
TTTGGTATTCGTCGAGAATA+TGG + chr2.1:58107034-58107053 MS.gene00194:CDS 35.0%
! AGGGTGAATTTTTGAATGCT+TGG + chr2.1:58107998-58108017 MS.gene00194:CDS 35.0%
! CTTTAGTGACTGTTTCCTTT+TGG - chr2.1:58107455-58107474 None:intergenic 35.0%
! GCCTTCTTCATTTTCAATCT+AGG - chr2.1:58108294-58108313 None:intergenic 35.0%
! GTATGGTTATTTCACTCGAT+CGG + chr2.1:58107191-58107210 MS.gene00194:intron 35.0%
! TATGTTGGCCAAAAAGTATG+TGG + chr2.1:58108806-58108825 MS.gene00194:CDS 35.0%
! TCTGAAATCATCACTTATGG+TGG + chr2.1:58106831-58106850 MS.gene00194:intron 35.0%
! TGATCCGTCAAAATGGTTTT+TGG - chr2.1:58107811-58107830 None:intergenic 35.0%
! TGCAGTCTAAGTTTTTCTAG+TGG + chr2.1:58106668-58106687 MS.gene00194:intron 35.0%
! TGTCGTAAATTTGAGACACA+TGG + chr2.1:58108504-58108523 MS.gene00194:CDS 35.0%
! TTCTCCATCCACATACTTTT+TGG - chr2.1:58108817-58108836 None:intergenic 35.0%
!! AAAACAACATTCTGAAGTCG+CGG - chr2.1:58106776-58106795 None:intergenic 35.0%
!! ATGAGTTTCTGAAGCTTTGT+TGG - chr2.1:58108730-58108749 None:intergenic 35.0%
!! CATAGGCAAACAGTCATTTT+TGG + chr2.1:58107703-58107722 MS.gene00194:intron 35.0%
!!! ACCCTTATTTGTTTTGCATG+TGG - chr2.1:58107647-58107666 None:intergenic 35.0%
!!! ACTGTTTCCTTTTGGTTTCA+AGG - chr2.1:58107447-58107466 None:intergenic 35.0%
AAAAACATTGGGGGAAAGGA+TGG - chr2.1:58106609-58106628 None:intergenic 40.0%
AAGTATGTGGATGGAGAATC+AGG + chr2.1:58108819-58108838 MS.gene00194:CDS 40.0%
ACAGTCACTAAAGTATGGTG+AGG + chr2.1:58107462-58107481 MS.gene00194:intron 40.0%
ACTATGATAATCCAACGGAG+AGG + chr2.1:58108439-58108458 MS.gene00194:CDS 40.0%
AGTATGTGGATGGAGAATCA+GGG + chr2.1:58108820-58108839 MS.gene00194:CDS 40.0%
ATGGACAAGAAATGCAAGGT+TGG + chr2.1:58108605-58108624 MS.gene00194:CDS 40.0%
ATTGTCTTGGGAGATGAATC+TGG + chr2.1:58107331-58107350 MS.gene00194:intron 40.0%
CAAAAGACGTCAACCAAAAG+GGG + chr2.1:58107132-58107151 MS.gene00194:CDS 40.0%
CAATGAGTTATGTCCTCGAA+TGG + chr2.1:58108695-58108714 MS.gene00194:CDS 40.0%
CATTGTACCGCTTCATGAAA+TGG - chr2.1:58108680-58108699 None:intergenic 40.0%
CCCATTCTAATTTCTGCTCT+AGG - chr2.1:58107160-58107179 None:intergenic 40.0%
CGAATCATATGAACATGCTC+AGG - chr2.1:58107274-58107293 None:intergenic 40.0%
GACAAAAGACGTCAACCAAA+AGG + chr2.1:58107130-58107149 MS.gene00194:CDS 40.0%
GATAGATGTACACGAAGATG+AGG + chr2.1:58107978-58107997 MS.gene00194:CDS 40.0%
GCCTAGAGCAGAAATTAGAA+TGG + chr2.1:58107156-58107175 MS.gene00194:CDS 40.0%
GCTGGACTCTTCATAAACTT+TGG - chr2.1:58108772-58108791 None:intergenic 40.0%
GGAAGTGATCAAGATTGGAA+AGG + chr2.1:58108627-58108646 MS.gene00194:CDS 40.0%
TATATATCCAAGCAAGCTGG+TGG + chr2.1:58107379-58107398 MS.gene00194:intron 40.0%
TCACTAAAGTATGGTGAGGT+GGG + chr2.1:58107466-58107485 MS.gene00194:intron 40.0%
TGGACAAGAAATGCAAGGTT+GGG + chr2.1:58108606-58108625 MS.gene00194:CDS 40.0%
TTCCAAGTGAGGTACATTGT+TGG - chr2.1:58106951-58106970 None:intergenic 40.0%
TTGATCAACAACAATGGCAG+CGG - chr2.1:58106414-58106433 None:intergenic 40.0%
! CATTTCATGGATTGTGCACT+TGG + chr2.1:58107875-58107894 MS.gene00194:intron 40.0%
! TACCTGCAGTGAATGTTTTC+CGG - chr2.1:58108113-58108132 None:intergenic 40.0%
! TTGGCCAAAAAGTATGTGGA+TGG + chr2.1:58108810-58108829 MS.gene00194:CDS 40.0%
AAATAAGGGTTATCGACCGC+TGG + chr2.1:58107657-58107676 MS.gene00194:intron 45.0%
AATAAGGGTTATCGACCGCT+GGG + chr2.1:58107658-58107677 MS.gene00194:intron 45.0%
AATGGGCATGGACATATGTC+CGG + chr2.1:58108091-58108110 MS.gene00194:CDS 45.0%
ACATTTCTCAAGGCAATGGG+TGG + chr2.1:58107778-58107797 MS.gene00194:intron 45.0%
AGCGATGGACAAGAAATGCA+AGG + chr2.1:58108601-58108620 MS.gene00194:CDS 45.0%
AGTCCAACAAATACTCCCAG+CGG - chr2.1:58107676-58107695 None:intergenic 45.0%
CAAAATGTGCAACACGGACA+AGG + chr2.1:58106917-58106936 MS.gene00194:CDS 45.0%
CCAACGGAGAGGATAGTTAT+GGG + chr2.1:58108450-58108469 MS.gene00194:CDS 45.0%
GCTGGTGGAATACAGATAGT+TGG + chr2.1:58107394-58107413 MS.gene00194:intron 45.0%
GGTGTCTTGATCCGTCAAAA+TGG - chr2.1:58107818-58107837 None:intergenic 45.0%
GGTTGGGAAGTGATCAAGAT+TGG + chr2.1:58108622-58108641 MS.gene00194:CDS 45.0%
GTATGTGGATGGAGAATCAG+GGG + chr2.1:58108821-58108840 MS.gene00194:CDS 45.0%
GTCACTAAAGTATGGTGAGG+TGG + chr2.1:58107465-58107484 MS.gene00194:intron 45.0%
GTGATCAAGATTGGAAAGGG+AGG + chr2.1:58108631-58108650 MS.gene00194:CDS 45.0%
TATGTGGATGGAGAATCAGG+GGG + chr2.1:58108822-58108841 MS.gene00194:CDS 45.0%
TCCAACGGAGAGGATAGTTA+TGG + chr2.1:58108449-58108468 MS.gene00194:CDS 45.0%
TGCCAACAATGTACCTCACT+TGG + chr2.1:58106946-58106965 MS.gene00194:CDS 45.0%
! AGAAATAGCCTTAGCCATCG+CGG - chr2.1:58107839-58107858 None:intergenic 45.0%
! GCTGCCATTGTTGTTGATCA+AGG + chr2.1:58106413-58106432 MS.gene00194:CDS 45.0%
!! AGTGTTGGTAGCAGGATGAT+GGG - chr2.1:58106354-58106373 None:intergenic 45.0%
!! GAACATGCTCAGGTTGAAGT+TGG - chr2.1:58107264-58107283 None:intergenic 45.0%
!! GAAGTTTGATTCTGAGGCAG+CGG + chr2.1:58106973-58106992 MS.gene00194:CDS 45.0%
!! TAGTGTTGGTAGCAGGATGA+TGG - chr2.1:58106355-58106374 None:intergenic 45.0%
AATGGGATGTTTGGCGCGTA+TGG + chr2.1:58107174-58107193 MS.gene00194:intron 50.0%
CCCATAACTATCCTCTCCGT+TGG - chr2.1:58108453-58108472 None:intergenic 50.0%
GTCCGGAAAACATTCACTGC+AGG + chr2.1:58108108-58108127 MS.gene00194:CDS 50.0%
! CTGTATTCCACCAGCTTGCT+TGG - chr2.1:58107389-58107408 None:intergenic 50.0%
! TGCTACAAAGAGGGTGTACG+AGG + chr2.1:58107103-58107122 MS.gene00194:CDS 50.0%
!! CATGCTCAGGTTGAAGTTGG+TGG - chr2.1:58107261-58107280 None:intergenic 50.0%
!! GGTTCGTTAGTGTTGGTAGC+AGG - chr2.1:58106362-58106381 None:intergenic 50.0%
!! GTGTTGGTAGCAGGATGATG+GGG - chr2.1:58106353-58106372 None:intergenic 50.0%
TCAAGACACCGCGATGGCTA+AGG + chr2.1:58107828-58107847 MS.gene00194:intron 55.0%
!! CGACCGCTGGGAGTATTTGT+TGG + chr2.1:58107670-58107689 MS.gene00194:intron 55.0%
!! TCTGCTCTAGGCTCCCCTTT+TGG - chr2.1:58107148-58107167 None:intergenic 55.0%
AACAACAATGGCAGCGGCGC+AGG - chr2.1:58106408-58106427 None:intergenic 60.0%
ACAACAATGGCAGCGGCGCA+GGG - chr2.1:58106407-58106426 None:intergenic 60.0%
GACGGATCAAGACACCGCGA+TGG + chr2.1:58107822-58107841 MS.gene00194:intron 60.0%
GGATGGAGAATCAGGGGGCA+TGG + chr2.1:58108827-58108846 MS.gene00194:CDS 60.0%
! GGCGGCGGGTTCGTTAGTGT+TGG - chr2.1:58106369-58106388 None:intergenic 65.0%
CAATGGCAGCGGCGCAGGGA+TGG - chr2.1:58106403-58106422 None:intergenic 70.0%
GCAGGGATGGTCGGAGACGG+CGG - chr2.1:58106390-58106409 None:intergenic 70.0%
GGCGCAGGGATGGTCGGAGA+CGG - chr2.1:58106393-58106412 None:intergenic 70.0%
ATGGTCGGAGACGGCGGCGG+CGG - chr2.1:58106384-58106403 None:intergenic 75.0%
GGCAGCGGCGCAGGGATGGT+CGG - chr2.1:58106399-58106418 None:intergenic 75.0%
GGGATGGTCGGAGACGGCGG+CGG - chr2.1:58106387-58106406 None:intergenic 75.0%
! TGGTCGGAGACGGCGGCGGC+GGG - chr2.1:58106383-58106402 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 58106341 58108887 58106341 ID=MS.gene00194
chr2.1 mRNA 58106341 58108887 58106341 ID=MS.gene00194.t1;Parent=MS.gene00194
chr2.1 exon 58106341 58106434 58106341 ID=MS.gene00194.t1.exon1;Parent=MS.gene00194.t1
chr2.1 CDS 58106341 58106434 58106341 ID=cds.MS.gene00194.t1;Parent=MS.gene00194.t1
chr2.1 exon 58106882 58107190 58106882 ID=MS.gene00194.t1.exon2;Parent=MS.gene00194.t1
chr2.1 CDS 58106882 58107190 58106882 ID=cds.MS.gene00194.t1;Parent=MS.gene00194.t1
chr2.1 exon 58107974 58108887 58107974 ID=MS.gene00194.t1.exon3;Parent=MS.gene00194.t1
chr2.1 CDS 58107974 58108887 58107974 ID=cds.MS.gene00194.t1;Parent=MS.gene00194.t1
Gene Sequence

>MS.gene00194

ATGAGCGAACCCCATCATCCTGCTACCAACACTAACGAACCCGCCGCCGCCGTCTCCGACCATCCCTGCGCCGCTGCCATTGTTGTTGATCAAGGTAATATAGTTTGCCTTCTCTATTTTATTATTTGCCGTCTCTTTATTACATGATGTTGTTTTACTGTGTTGATGAAAAATTAGTTTCTAGGTTCACTTACGTGATGCATTACTTACATGATATACATATATATAGCTTTATGGTCACAATGTGTGTAACTTTATATAATCACCATCCTTTCCCCCAATGTTTTTTTAATAGTAATAGATAGCTTTATGGTCAACAAATTCTTATGCAGTCTAAGTTTTTCTAGTGGCTTTTTGCTGTTATGTTGTTTTTGTTCAGTTGTTGTCAAAAAATCATCAGTTGTTTCTGTTTATGTTGTTTCTGTTCAGTTGCCGCGACTTCAGAATGTTGTTTTCTAACACTGTTATCATATATTCATGTAAGTTTATGTCTGAAATCATCACTTATGGTGGCCTTAATTGTAAATTTTGGTTTTTTCAGATTGTGTCAATAAATTGGTTAACAACATTCATGAACAAAATGTGCAACACGGACAAGGAAGTATTGCCAACAATGTACCTCACTTGGAAATGAAGTTTGATTCTGAGGCAGCGGCTTATGACTTTTATAATGAGTATAGTAAAAGAATTGGATTTGGTATTCGTCGAGAATATGGAAATAAAAGCAGAGTTGATGGAGTTTTGACTTCAAGAAGATTCACATGCTACAAAGAGGGTGTACGAGGTGTTGACAAAAGACGTCAACCAAAAGGGGAGCCTAGAGCAGAAATTAGAATGGGATGTTTGGCGCGTATGGTTATTTCACTCGATCGGAAGATAGAGAAATATAAAGTTGTTGACTTTATAGCTGAACATAACCACCAACTTCAACCTGAGCATGTTCATATGATTCGCTCTCATCGACGCATATCTGAGACACAAGCATCACAAATTGTCTTGGGAGATGAATCTGGATTAAGACCAAAAGATTTGCATGAATATATATCCAAGCAAGCTGGTGGAATACAGATAGTTGGTTTTACAAAAAAAGTCCTTACGAATTACCTTGAAACCAAAAGGAAACAGTCACTAAAGTATGGTGAGGTGGGTGCACTGATGATGTATTTCAAAAAAGAAAGTGAAAATCCCTCGTTCTATTATGACTTTCAAATGGATGTTGAAGAGCAAATAACAAATATATTTTGGGCGGATGCACAAATGATAAATGATTATGGGTATTTTGGTGATGTTATTACTTTTGACACCACATGCAAAACAAATAAGGGTTATCGACCGCTGGGAGTATTTGTTGGACTAAATAATCATAGGCAAACAGTCATTTTTGGTGCAGCATTGTTATATGACGAAACAATCCCTTCTTTCAAGTGGTTATTTGAAACATTTCTCAAGGCAATGGGTGGTAAAAAGCCAAAAACCATTTTGACGGATCAAGACACCGCGATGGCTAAGGCTATTTCTTTAGTTATGCCAGAAACATTTCATGGATTGTGCACTTGGCATATAAGACAGAATGCTTTACGGCATGTGAACCACTTATATCAAAAGAGTTCACAATTCGGTTTGGATTTTGAAGCATTGATAGATGTACACGAAGATGAGGGTGAATTTTTGAATGCTTGGAATTCTTTACTTGTTGAGCATAATATATTGGAAGGGTCGTGGTTGCATACAATTTATCAATTGAAGGAAAAATGGGCATGGACATATGTCCGGAAAACATTCACTGCAGGTATGAGATCTACTCAATTAAGTGAGAGCTTTAATGCTGATTTGAAGAACCATTTGAAGTCTGATTTAAATTTGGTACAATTTTTTACACACTTTAAAAGAGCTGTTAATGATAAACGAAACAAAGAGTCAGAAGCTGAATATGAGTCAAAAAATAAGCTGCCTAGATTGAAAATGAAGAAGGCTCGTATGCTCATACAAGCTGCAAATGTTTACACTCCTAGAATATTTGAGGAGTTCCAAGAGGAATATGAAGAATATCAAGACACTTGCGTAAAAGATTTGAAAGAAGGTTTGTATGCTGTCACAAACTATGATAATCCAACGGAGAGGATAGTTATGGGAAATGCTATGGAACAAAAAGTTTCATGTGATTGTCGTAAATTTGAGACACATGGCATCTTATGCAGTCATGCTTTGAAAGTTTTAGATGTCATGAACAGTAAGTTAATTCCTGAAACCTATATACTTAAGCGATGGACAAGAAATGCAAGGTTGGGAAGTGATCAAGATTGGAAAGGGAGGCATGTTGAATTAGACATAAAAGCCCATTTCATGAAGCGGTACAATGAGTTATGTCCTCGAATGGTTAAGTTAACCAACAAAGCTTCAGAAACTCATGAAACTTACACATTTTTGTCCAAAGTTTATGAAGAGTCCAGCAAAATAATTGATGATATGTTGGCCAAAAAGTATGTGGATGGAGAATCAGGGGGCATGGTTCATGTATCCATATCAATTGCAAATGATGAAATTTAA
Protein sequence

>MS.gene00194.t1

MSEPHHPATNTNEPAAAVSDHPCAAAIVVDQDCVNKLVNNIHEQNVQHGQGSIANNVPHLEMKFDSEAAAYDFYNEYSKRIGFGIRREYGNKSRVDGVLTSRRFTCYKEGVRGVDKRRQPKGEPRAEIRMGCLAPLIDVHEDEGEFLNAWNSLLVEHNILEGSWLHTIYQLKEKWAWTYVRKTFTAGMRSTQLSESFNADLKNHLKSDLNLVQFFTHFKRAVNDKRNKESEAEYESKNKLPRLKMKKARMLIQAANVYTPRIFEEFQEEYEEYQDTCVKDLKEGLYAVTNYDNPTERIVMGNAMEQKVSCDCRKFETHGILCSHALKVLDVMNSKLIPETYILKRWTRNARLGSDQDWKGRHVELDIKAHFMKRYNELCPRMVKLTNKASETHETYTFLSKVYEESSKIIDDMLAKKYVDGESGGMVHVSISIANDEI