AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene001948

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001948.t1 MTR_7g028740 97.011 368 8 3 1 366 1 367 0.0 735
MS.gene001948.t1 MTR_3g099080 35.176 398 204 12 1 366 1 376 1.13e-58 194
MS.gene001948.t1 MTR_8g096780 48.756 201 95 6 17 211 36 234 2.85e-50 173
MS.gene001948.t1 MTR_4g109770 54.264 129 51 3 25 150 75 198 3.56e-37 140
MS.gene001948.t1 MTR_2g461920 52.308 130 60 1 70 199 166 293 3.79e-35 135
MS.gene001948.t1 MTR_3g085180 59.341 91 34 1 67 154 154 244 6.19e-33 127
MS.gene001948.t1 MTR_5g046670 57.000 100 43 0 74 173 243 342 8.34e-33 128
MS.gene001948.t1 MTR_7g092930 63.636 88 32 0 70 157 63 150 8.93e-33 125
MS.gene001948.t1 MTR_4g088555 59.551 89 34 1 62 148 48 136 4.25e-32 118
MS.gene001948.t1 MTR_8g463140 63.750 80 29 0 74 153 64 143 6.68e-32 119
MS.gene001948.t1 MTR_2g014200 64.198 81 29 0 69 149 58 138 5.00e-31 115
MS.gene001948.t1 MTR_1g053715 62.651 83 31 0 75 157 74 156 6.77e-31 120
MS.gene001948.t1 MTR_1g035010 43.046 151 70 4 2 151 45 180 1.68e-30 123
MS.gene001948.t1 MTR_2g046550 45.926 135 58 2 17 151 96 215 2.79e-30 122
MS.gene001948.t1 MTR_7g444860 63.750 80 29 0 75 154 149 228 3.35e-30 117
MS.gene001948.t1 MTR_2g078770 66.667 75 25 0 74 148 57 131 1.05e-29 112
MS.gene001948.t1 MTR_8g005960 56.436 101 39 2 58 153 165 265 3.12e-29 115
MS.gene001948.t1 MTR_1g086250 60.440 91 35 1 59 148 130 220 1.12e-28 118
MS.gene001948.t1 MTR_7g110320 59.551 89 34 1 62 148 130 218 2.37e-28 117
MS.gene001948.t1 MTR_7g110320 59.551 89 34 1 62 148 130 218 2.86e-28 117
MS.gene001948.t1 MTR_8g080690 62.338 77 29 0 75 151 151 227 4.18e-28 113
MS.gene001948.t1 MTR_8g080680 56.757 74 32 0 75 148 132 205 4.76e-24 102
MS.gene001948.t1 MTR_8g080670 50.575 87 39 1 65 147 148 234 7.71e-24 102
MS.gene001948.t1 MTR_2g020620 49.231 65 33 0 84 148 99 163 2.24e-16 81.3
MS.gene001948.t1 MTR_8g066240 44.211 95 49 2 25 115 8 102 1.80e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001948.t1 AT5G50670 46.354 192 87 5 24 200 41 231 2.28e-43 154
MS.gene001948.t1 AT5G50670 46.354 192 87 5 24 200 41 231 2.28e-43 154
MS.gene001948.t1 AT5G50570 46.354 192 87 5 24 200 41 231 2.28e-43 154
MS.gene001948.t1 AT5G50570 46.354 192 87 5 24 200 41 231 2.28e-43 154
MS.gene001948.t1 AT1G69170 67.416 89 28 1 62 149 110 198 4.76e-34 130
MS.gene001948.t1 AT1G76580 44.156 154 85 1 2 154 40 193 1.78e-32 129
MS.gene001948.t1 AT1G76580 44.156 154 85 1 2 154 40 193 1.82e-32 129
MS.gene001948.t1 AT1G76580 44.156 154 85 1 2 154 40 193 2.22e-32 129
MS.gene001948.t1 AT2G42200 63.218 87 32 0 75 161 74 160 3.91e-32 124
MS.gene001948.t1 AT5G43270 47.788 113 59 0 75 187 169 281 4.91e-32 125
MS.gene001948.t1 AT5G43270 47.788 113 59 0 75 187 169 281 4.91e-32 125
MS.gene001948.t1 AT5G43270 47.788 113 59 0 75 187 169 281 4.91e-32 125
MS.gene001948.t1 AT1G20980 44.375 160 69 3 2 152 49 197 8.10e-32 127
MS.gene001948.t1 AT3G60030 59.375 96 37 1 61 154 111 206 2.08e-31 126
MS.gene001948.t1 AT1G27370 56.075 107 43 2 48 150 145 251 4.01e-31 121
MS.gene001948.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.80e-31 121
MS.gene001948.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.80e-31 121
MS.gene001948.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.80e-31 121
MS.gene001948.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.80e-31 121
MS.gene001948.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.80e-31 121
MS.gene001948.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.80e-31 121
MS.gene001948.t1 AT2G33810 62.667 75 28 0 74 148 53 127 7.01e-31 114
MS.gene001948.t1 AT1G27360 67.105 76 25 0 75 150 175 250 6.93e-30 118
MS.gene001948.t1 AT1G27360 67.105 76 25 0 75 150 175 250 6.93e-30 118
MS.gene001948.t1 AT1G27360 67.105 76 25 0 75 150 175 250 6.93e-30 118
MS.gene001948.t1 AT1G27360 67.105 76 25 0 75 150 175 250 6.93e-30 118
MS.gene001948.t1 AT1G53160 52.688 93 44 0 63 155 42 134 2.55e-29 111
MS.gene001948.t1 AT1G53160 52.688 93 44 0 63 155 42 134 4.51e-29 111
MS.gene001948.t1 AT1G53160 52.688 93 44 0 63 155 42 134 4.51e-29 111
MS.gene001948.t1 AT2G47070 65.385 78 27 0 74 151 105 182 5.56e-29 119
MS.gene001948.t1 AT3G15270 61.333 75 29 0 74 148 62 136 2.16e-28 109
MS.gene001948.t1 AT3G57920 51.961 102 44 2 75 176 59 155 2.25e-28 114
MS.gene001948.t1 AT1G02065 64.865 74 26 0 75 148 188 261 7.98e-28 112
MS.gene001948.t1 AT5G18830 48.315 89 45 1 62 149 125 213 5.11e-21 95.1
MS.gene001948.t1 AT5G18830 48.315 89 45 1 62 149 125 213 6.49e-21 94.7
MS.gene001948.t1 AT5G18830 48.315 89 45 1 62 149 125 213 6.60e-21 94.7
MS.gene001948.t1 AT2G47070 73.077 52 14 0 100 151 2 53 5.92e-17 82.8
MS.gene001948.t1 AT1G02065 45.570 79 36 2 58 129 166 244 3.50e-15 74.7

Find 90 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCTTATCTTTCGCCTTTC+CGG 0.146373 7.3:+72048449 None:intergenic
CAGTGACTCTTCCATTCTTC+TGG 0.209211 7.3:-72048329 MS.gene001948:CDS
GAGAAATCAGTTCATGATTC+AGG 0.275617 7.3:-72050746 MS.gene001948:CDS
CTACCAAAGTGCAGGATTCT+AGG 0.315434 7.3:+72048900 None:intergenic
GGCCAAATGTTTCTCATAAT+AGG 0.321028 7.3:+72048858 None:intergenic
CTCTCTTCTGATGGCAAGCC+CGG 0.326414 7.3:-72048624 MS.gene001948:CDS
GGATTTGAGGCTTGGTGATC+AGG 0.330157 7.3:-72050783 MS.gene001948:CDS
ATGAGGTTAAGAGGAGTTGC+AGG 0.333125 7.3:-72050091 MS.gene001948:CDS
GGTTCATCCGCAGGTGAAAA+AGG 0.348606 7.3:-72048657 MS.gene001948:CDS
AAAAGAGCGCGTTTGCAAAA+TGG 0.355601 7.3:-72050674 MS.gene001948:CDS
TCAGCCACCTTCTCTCTTCA+TGG 0.360485 7.3:-72050030 MS.gene001948:CDS
GGACCGGTATTTGTTCTAGA+TGG 0.361540 7.3:-72048423 MS.gene001948:CDS
TCATATACCTGCTGCATTGT+TGG 0.364223 7.3:+72050510 None:intergenic
GAGAGTATCATAAGCGTCAT+AGG 0.369267 7.3:-72050595 MS.gene001948:CDS
GCTTCAGTTATGGAAGTTGC+AGG 0.374630 7.3:+72048837 None:intergenic
CGACAAACATAAACAGGATA+AGG 0.380487 7.3:-72048775 MS.gene001948:CDS
TGATCAGGCTTCTGATTCAT+TGG 0.384148 7.3:-72050768 MS.gene001948:CDS
TCCATAACTGAAGCTGAATC+TGG 0.398324 7.3:-72048828 MS.gene001948:CDS
GCAGCATCCCATTGCAATGA+AGG 0.401478 7.3:+72048388 None:intergenic
CCGCGGATGGGATGGCTGAT+AGG 0.406880 7.3:+72048687 None:intergenic
TTGTTAACATCGCCGGACTT+AGG 0.408591 7.3:+72048726 None:intergenic
AAAACTCCTGTTGTGTTGGT+TGG 0.411330 7.3:-72050554 MS.gene001948:CDS
ACCAGATTCAGCTTCAGTTA+TGG 0.414958 7.3:+72048827 None:intergenic
TCGAATCGACAAACATAAAC+AGG 0.415548 7.3:-72048781 MS.gene001948:CDS
TGTTAACATCGCCGGACTTA+GGG 0.417871 7.3:+72048727 None:intergenic
AGTGACTCTTCCATTCTTCT+GGG 0.418501 7.3:-72048328 MS.gene001948:CDS
GGGATTGGAAAGAGTTTGCT+TGG 0.421291 7.3:-72050850 MS.gene001948:CDS
CGGAGTTTGATGAGGTTAAG+AGG 0.427340 7.3:-72050100 MS.gene001948:CDS
TGCTTGGGATCCATCTGGAT+TGG 0.431081 7.3:-72050834 MS.gene001948:CDS
AATATGATTTGCTCGGTTGA+TGG 0.434096 7.3:-72050644 MS.gene001948:CDS
GGATGGCTGATAGGAGTTCC+AGG 0.435057 7.3:+72048696 None:intergenic
GAGTTTGCTTGGGATCCATC+TGG 0.438111 7.3:-72050839 MS.gene001948:CDS
ATCCTATTATGAGAAACATT+TGG 0.440090 7.3:-72048860 MS.gene001948:CDS
ATGCTGCAAATGGGTCCTAA+TGG 0.447508 7.3:-72048372 MS.gene001948:CDS
CAACAAACCTTCATTGCAAT+GGG 0.452275 7.3:-72048395 MS.gene001948:CDS
TTGGGATCCATCTGGATTGG+AGG 0.460222 7.3:-72050831 MS.gene001948:CDS
TTGCAATGGGATGCTGCAAA+TGG 0.467365 7.3:-72048382 MS.gene001948:CDS
TGAACCAACGGCACCGCGGA+TGG 0.474180 7.3:+72048674 None:intergenic
ACAACCCGAGTCAAAAGATC+CGG 0.476548 7.3:+72048605 None:intergenic
GACAAACATAAACAGGATAA+GGG 0.477832 7.3:-72048774 MS.gene001948:CDS
TGCTGCTTTGTTAACATCGC+CGG 0.478377 7.3:+72048719 None:intergenic
CATCCCATCCGCGGTGCCGT+TGG 0.480127 7.3:-72048678 MS.gene001948:CDS
ACGAAGGAGTACTCGTACTC+CGG 0.480410 7.3:-72048468 MS.gene001948:CDS
AGTTGCAGGAAACGTCTTGA+TGG 0.488371 7.3:-72050077 MS.gene001948:CDS
TTCTAAAACTCCTGTTGTGT+TGG 0.493907 7.3:-72050558 MS.gene001948:CDS
AGAACAAATACCGGTCCATT+CGG 0.494466 7.3:+72048429 None:intergenic
AGGATAAGGGACATCCTCAT+TGG 0.499189 7.3:-72048761 MS.gene001948:CDS
CAACGGCACCGCGGATGGGA+TGG 0.499675 7.3:+72048679 None:intergenic
GATTCAATGGATTTGAGGCT+TGG 0.506642 7.3:-72050791 MS.gene001948:CDS
AGACGCTACTCCCAGAAGAA+TGG 0.507419 7.3:+72048318 None:intergenic
GGATTGGAAAGAGTTTGCTT+GGG 0.508642 7.3:-72050849 MS.gene001948:CDS
TTACCTCCAACCAACACAAC+AGG 0.509843 7.3:+72050548 None:intergenic
GAACCAACGGCACCGCGGAT+GGG 0.516027 7.3:+72048675 None:intergenic
GAAAGATAAGCCGAATGGAC+CGG 0.517456 7.3:-72048439 MS.gene001948:CDS
ACTCCTGTTGTGTTGGTTGG+AGG 0.522154 7.3:-72050551 MS.gene001948:CDS
ACAACAAACCTTCATTGCAA+TGG 0.522232 7.3:-72048396 MS.gene001948:CDS
ATGAAGAGAGAAGGTGGCTG+AGG 0.523686 7.3:+72050032 None:intergenic
AAGGTGATTCAATGGATTTG+AGG 0.524985 7.3:-72050796 MS.gene001948:CDS
AGTCCAACACAATCTTCGTC+TGG 0.529463 7.3:-72048510 MS.gene001948:CDS
CAAGAAATTCTCCCTAAGTC+CGG 0.531212 7.3:-72048738 MS.gene001948:CDS
TTCACCTGCGGATGAACCAA+CGG 0.533214 7.3:+72048662 None:intergenic
TTCAACTCCTCCAATCCAGA+TGG 0.535266 7.3:+72050824 None:intergenic
GGGAGAATTTCTTGCCAATG+AGG 0.536926 7.3:+72048747 None:intergenic
GGTGCCGTTGGTTCATCCGC+AGG 0.539865 7.3:-72048666 MS.gene001948:CDS
GGACCTAGAATCCTGCACTT+TGG 0.540161 7.3:-72048903 MS.gene001948:CDS
TGCAATGGGATGCTGCAAAT+GGG 0.554526 7.3:-72048381 MS.gene001948:CDS
GGTTCTGCCAACAATGCAGC+AGG 0.557731 7.3:-72050517 MS.gene001948:intron
ATGTAGGTTTCATTCACTTG+CGG 0.558210 7.3:-72050120 MS.gene001948:intron
TCTGAGGGCTACCAAAGTGC+AGG 0.563556 7.3:+72048892 None:intergenic
GATGTTAACAAAGCAGCACC+TGG 0.566098 7.3:-72048714 MS.gene001948:CDS
GGAGTACTCGTACTCCGGAA+AGG 0.575335 7.3:-72048463 MS.gene001948:CDS
ATCGCAGAATATGATTTGCT+CGG 0.580677 7.3:-72050651 MS.gene001948:CDS
GGTAGGAAGCTCTCTTCTGA+TGG 0.581427 7.3:-72048633 MS.gene001948:CDS
TTTCCATCTAGAACAAATAC+CGG 0.581750 7.3:+72048420 None:intergenic
ATCAGTTCATGATTCAGGAA+AGG 0.585059 7.3:-72050741 MS.gene001948:CDS
AGAGTATCATAAGCGTCATA+GGG 0.590533 7.3:-72050594 MS.gene001948:CDS
TTCACTTGCGGAGTTTGATG+AGG 0.591231 7.3:-72050108 MS.gene001948:CDS
TGGTTGGAGGTAAGCAGCAG+AGG 0.595809 7.3:-72050538 MS.gene001948:CDS
TCAGCAGCCATGAAGAGAGA+AGG 0.603814 7.3:+72050023 None:intergenic
CAACCCGAGTCAAAAGATCC+GGG 0.610877 7.3:+72048606 None:intergenic
GCAGCCATGAAGAGAGAAGG+TGG 0.612633 7.3:+72050026 None:intergenic
TAGGATTGACATATGTCTGA+GGG 0.617179 7.3:+72048877 None:intergenic
GTTCCAGACGAAGATTGTGT+TGG 0.619281 7.3:+72048507 None:intergenic
AAGTTCATGTACAATTACAA+AGG 0.619439 7.3:-72049999 MS.gene001948:intron
ATAGGATTGACATATGTCTG+AGG 0.620773 7.3:+72048876 None:intergenic
TTGATGGACATAATAAGCGC+AGG 0.628418 7.3:-72050061 MS.gene001948:CDS
AAGGCGAAAGATAAGCCGAA+TGG 0.638149 7.3:-72048444 MS.gene001948:CDS
CCTATCAGCCATCCCATCCG+CGG 0.652776 7.3:-72048687 MS.gene001948:CDS
ATGGACATAATAAGCGCAGG+AGG 0.679251 7.3:-72050058 MS.gene001948:CDS
CGGATGAACCAACGGCACCG+CGG 0.746313 7.3:+72048670 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTCTCTTTGTACAATTTT+AGG - chr7.3:72050256-72050275 MS.gene001948:intron 20.0%
! AAGTTCATGTACAATTACAA+AGG - chr7.3:72049181-72049200 MS.gene001948:intron 25.0%
! AATGAGATGAAAACAACTTA+TGG - chr7.3:72048900-72048919 MS.gene001948:CDS 25.0%
! ATATTCAGAGAGCTTATATA+TGG + chr7.3:72048944-72048963 None:intergenic 25.0%
! ATCCTATTATGAGAAACATT+TGG - chr7.3:72050320-72050339 MS.gene001948:intron 25.0%
! ATTCTATTGTCAAAACCTTA+TGG + chr7.3:72049390-72049409 None:intergenic 25.0%
! CATATACATAACAATCCATA+AGG - chr7.3:72049372-72049391 MS.gene001948:intron 25.0%
! CATTAAATTTGATTAGAGAG+AGG + chr7.3:72049811-72049830 None:intergenic 25.0%
! CTTTCTTGTGAAAATAAAGA+CGG + chr7.3:72049851-72049870 None:intergenic 25.0%
! TGAATTGAAGTTGTTTGTTT+CGG - chr7.3:72048690-72048709 MS.gene001948:CDS 25.0%
! TGGATTTCAATTGCAAAAAA+TGG - chr7.3:72049909-72049928 MS.gene001948:intron 25.0%
!! CTCTCTGAATATTCTAAAAA+TGG - chr7.3:72048952-72048971 MS.gene001948:intron 25.0%
!! TATAGTTATTTAGCGACTTT+TGG - chr7.3:72049486-72049505 MS.gene001948:intron 25.0%
!! TTTTTGCAATTGAAATCCAT+GGG + chr7.3:72049908-72049927 None:intergenic 25.0%
!!! CGAAGATATAACGTTTTTTA+TGG - chr7.3:72049315-72049334 MS.gene001948:intron 25.0%
!!! CTTTATTTTCACAAGAAAGA+TGG - chr7.3:72049852-72049871 MS.gene001948:intron 25.0%
!!! TTGAGGTTGTTTTGTAATAT+GGG - chr7.3:72049024-72049043 MS.gene001948:intron 25.0%
!!! TTTGAGGTTGTTTTGTAATA+TGG - chr7.3:72049023-72049042 MS.gene001948:intron 25.0%
!!! TTTTTTGCAATTGAAATCCA+TGG + chr7.3:72049909-72049928 None:intergenic 25.0%
AATTTGATTAGAGAGAGGAT+TGG + chr7.3:72049806-72049825 None:intergenic 30.0%
AGTAACTGTTGGATTTAGAT+CGG - chr7.3:72049961-72049980 MS.gene001948:intron 30.0%
ATTAGTGCTTTAGTAACTGT+TGG - chr7.3:72049950-72049969 MS.gene001948:intron 30.0%
GAAAAATGAAGGTGATTCAA+TGG - chr7.3:72048376-72048395 MS.gene001948:CDS 30.0%
GAATTGAAGTTGTTTGTTTC+GGG - chr7.3:72048691-72048710 MS.gene001948:CDS 30.0%
GACAAACATAAACAGGATAA+GGG - chr7.3:72050406-72050425 MS.gene001948:intron 30.0%
TACAAAACAACCTCAAAACT+CGG + chr7.3:72049020-72049039 None:intergenic 30.0%
TTAGTTAAGTTTGAACATGC+CGG + chr7.3:72050140-72050159 None:intergenic 30.0%
! TATTTTCACAAGAAAGATGG+TGG - chr7.3:72049855-72049874 MS.gene001948:intron 30.0%
! TCATTTTCGATCAATCCATT+AGG + chr7.3:72050826-72050845 None:intergenic 30.0%
! TTTCCATCTAGAACAAATAC+CGG + chr7.3:72050763-72050782 None:intergenic 30.0%
! TTTTGCAATTGAAATCCATG+GGG + chr7.3:72049907-72049926 None:intergenic 30.0%
!! TCTCATGATTCTAAGATAGA+TGG - chr7.3:72049219-72049238 MS.gene001948:intron 30.0%
!! TGAGGTTGTTTTGTAATATG+GGG - chr7.3:72049025-72049044 MS.gene001948:intron 30.0%
ACAACAAACCTTCATTGCAA+TGG - chr7.3:72050784-72050803 MS.gene001948:CDS 35.0%
ACTTTGCTAATATTTCCCCA+TGG - chr7.3:72049889-72049908 MS.gene001948:intron 35.0%
AGGGAATGCATAAAAACTCT+GGG - chr7.3:72049589-72049608 MS.gene001948:intron 35.0%
ATAATTCCCTCTTTCATCTC+AGG + chr7.3:72049293-72049312 None:intergenic 35.0%
ATAGGATTGACATATGTCTG+AGG + chr7.3:72050307-72050326 None:intergenic 35.0%
ATCAGTTCATGATTCAGGAA+AGG - chr7.3:72048439-72048458 MS.gene001948:CDS 35.0%
ATGTAGGTTTCATTCACTTG+CGG - chr7.3:72049060-72049079 MS.gene001948:intron 35.0%
CAACAAACCTTCATTGCAAT+GGG - chr7.3:72050785-72050804 MS.gene001948:CDS 35.0%
CGACAAACATAAACAGGATA+AGG - chr7.3:72050405-72050424 MS.gene001948:intron 35.0%
CTTGTGAAAATAAAGACGGA+AGG + chr7.3:72049847-72049866 None:intergenic 35.0%
GAATCTTGATCCTTGATTTC+CGG - chr7.3:72050118-72050137 MS.gene001948:intron 35.0%
GAGAAATCAGTTCATGATTC+AGG - chr7.3:72048434-72048453 MS.gene001948:CDS 35.0%
GCTTAGTGCAATAAGTTTCT+AGG - chr7.3:72049626-72049645 MS.gene001948:intron 35.0%
GGCCAAATGTTTCTCATAAT+AGG + chr7.3:72050325-72050344 None:intergenic 35.0%
TAAACACTTGCGCAAGTATT+TGG + chr7.3:72049718-72049737 None:intergenic 35.0%
TAGGATTGACATATGTCTGA+GGG + chr7.3:72050306-72050325 None:intergenic 35.0%
TCGAATCGACAAACATAAAC+AGG - chr7.3:72050399-72050418 MS.gene001948:intron 35.0%
TTAGCTCAGAATTCAGTTCA+TGG + chr7.3:72049452-72049471 None:intergenic 35.0%
TTGGAGGAGTTGAAAAATGA+AGG - chr7.3:72048365-72048384 MS.gene001948:CDS 35.0%
! AAGGTGATTCAATGGATTTG+AGG - chr7.3:72048384-72048403 MS.gene001948:CDS 35.0%
! AGAGTATCATAAGCGTCATA+GGG - chr7.3:72048586-72048605 MS.gene001948:CDS 35.0%
! AGCTGATTCTACTATTGAAG+AGG - chr7.3:72049569-72049588 MS.gene001948:intron 35.0%
! GCTGATTCTACTATTGAAGA+GGG - chr7.3:72049570-72049589 MS.gene001948:intron 35.0%
!! AATATGATTTGCTCGGTTGA+TGG - chr7.3:72048536-72048555 MS.gene001948:CDS 35.0%
!! ATCGCAGAATATGATTTGCT+CGG - chr7.3:72048529-72048548 MS.gene001948:CDS 35.0%
!! TTCTAAAACTCCTGTTGTGT+TGG - chr7.3:72048622-72048641 MS.gene001948:CDS 35.0%
ACCAGATTCAGCTTCAGTTA+TGG + chr7.3:72050356-72050375 None:intergenic 40.0%
ACCTCAAAACTCGGTATGTT+TGG + chr7.3:72049011-72049030 None:intergenic 40.0%
AGAACAAATACCGGTCCATT+CGG + chr7.3:72050754-72050773 None:intergenic 40.0%
AGCACTAATCGTGTAAATGC+AGG + chr7.3:72049939-72049958 None:intergenic 40.0%
AGTGACTCTTCCATTCTTCT+GGG - chr7.3:72050852-72050871 MS.gene001948:CDS 40.0%
CAAGAAATTCTCCCTAAGTC+CGG - chr7.3:72050442-72050461 MS.gene001948:intron 40.0%
CAATCTTGATCCAACAGTCA+CGG - chr7.3:72050055-72050074 MS.gene001948:CDS 40.0%
GAGGGAATGCATAAAAACTC+TGG - chr7.3:72049588-72049607 MS.gene001948:intron 40.0%
GATCAAGATTCTCTGCAGTT+GGG + chr7.3:72050109-72050128 None:intergenic 40.0%
GCACTAATCGTGTAAATGCA+GGG + chr7.3:72049938-72049957 None:intergenic 40.0%
GTCAAACCTGAGATGAAAGA+GGG - chr7.3:72049284-72049303 MS.gene001948:intron 40.0%
TACTGTGCTGAAAATTCGCA+CGG - chr7.3:72050030-72050049 MS.gene001948:CDS 40.0%
TCATATACCTGCTGCATTGT+TGG + chr7.3:72048673-72048692 None:intergenic 40.0%
TCCATAACTGAAGCTGAATC+TGG - chr7.3:72050352-72050371 MS.gene001948:intron 40.0%
TGATCAGGCTTCTGATTCAT+TGG - chr7.3:72048412-72048431 MS.gene001948:CDS 40.0%
TGTCAAACCTGAGATGAAAG+AGG - chr7.3:72049283-72049302 MS.gene001948:intron 40.0%
TTCCACAAGCACAGGTAATA+TGG - chr7.3:72050220-72050239 MS.gene001948:intron 40.0%
TTTGAACATGCCGGAAATCA+AGG + chr7.3:72050131-72050150 None:intergenic 40.0%
! AAAACTCCTGTTGTGTTGGT+TGG - chr7.3:72048626-72048645 MS.gene001948:CDS 40.0%
! AAAAGAGCGCGTTTGCAAAA+TGG - chr7.3:72048506-72048525 MS.gene001948:CDS 40.0%
! GATTCAATGGATTTGAGGCT+TGG - chr7.3:72048389-72048408 MS.gene001948:CDS 40.0%
! GCTCTTTTCAACGATCTAGT+AGG + chr7.3:72048494-72048513 None:intergenic 40.0%
! GGATTGGAAAGAGTTTGCTT+GGG - chr7.3:72048331-72048350 MS.gene001948:CDS 40.0%
! GGGGTGGTTCAATTTTATGT+AGG - chr7.3:72049044-72049063 MS.gene001948:intron 40.0%
! TCCAAACATACCGAGTTTTG+AGG - chr7.3:72049007-72049026 MS.gene001948:intron 40.0%
! TTGATGGACATAATAAGCGC+AGG - chr7.3:72049119-72049138 MS.gene001948:intron 40.0%
! TTTTTGTCTTCCACAAGCAC+AGG - chr7.3:72050212-72050231 MS.gene001948:intron 40.0%
!! GAGAGTATCATAAGCGTCAT+AGG - chr7.3:72048585-72048604 MS.gene001948:CDS 40.0%
!! GGTTGTTTTGTAATATGGGG+TGG - chr7.3:72049028-72049047 MS.gene001948:intron 40.0%
!! GTTGCATTTTGATGCTACGA+AGG - chr7.3:72050696-72050715 MS.gene001948:CDS 40.0%
AAGGCGAAAGATAAGCCGAA+TGG - chr7.3:72050736-72050755 MS.gene001948:CDS 45.0%
ACAACCCGAGTCAAAAGATC+CGG + chr7.3:72050578-72050597 None:intergenic 45.0%
AGGATAAGGGACATCCTCAT+TGG - chr7.3:72050419-72050438 MS.gene001948:intron 45.0%
AGTCCAACACAATCTTCGTC+TGG - chr7.3:72050670-72050689 MS.gene001948:CDS 45.0%
AGTTGCAGGAAACGTCTTGA+TGG - chr7.3:72049103-72049122 MS.gene001948:intron 45.0%
ATGAGGTTAAGAGGAGTTGC+AGG - chr7.3:72049089-72049108 MS.gene001948:intron 45.0%
ATGCTGCAAATGGGTCCTAA+TGG - chr7.3:72050808-72050827 MS.gene001948:CDS 45.0%
ATGGACATAATAAGCGCAGG+AGG - chr7.3:72049122-72049141 MS.gene001948:intron 45.0%
CAGTGACTCTTCCATTCTTC+TGG - chr7.3:72050851-72050870 MS.gene001948:CDS 45.0%
CGGAGTTTGATGAGGTTAAG+AGG - chr7.3:72049080-72049099 MS.gene001948:intron 45.0%
CGTGACTGTTGGATCAAGAT+TGG + chr7.3:72050057-72050076 None:intergenic 45.0%
CTACCAAAGTGCAGGATTCT+AGG + chr7.3:72050283-72050302 None:intergenic 45.0%
CTCCATATTACCTGTGCTTG+TGG + chr7.3:72050225-72050244 None:intergenic 45.0%
GAAAGATAAGCCGAATGGAC+CGG - chr7.3:72050741-72050760 MS.gene001948:CDS 45.0%
GATGTTAACAAAGCAGCACC+TGG - chr7.3:72050466-72050485 MS.gene001948:intron 45.0%
GCTTCAGTTATGGAAGTTGC+AGG + chr7.3:72050346-72050365 None:intergenic 45.0%
GGATCAAGATTCTCTGCAGT+TGG + chr7.3:72050110-72050129 None:intergenic 45.0%
GGGAGAATTTCTTGCCAATG+AGG + chr7.3:72050436-72050455 None:intergenic 45.0%
GTTCCAGACGAAGATTGTGT+TGG + chr7.3:72050676-72050695 None:intergenic 45.0%
TGCAATGGGATGCTGCAAAT+GGG - chr7.3:72050799-72050818 MS.gene001948:CDS 45.0%
TGTTAACATCGCCGGACTTA+GGG + chr7.3:72050456-72050475 None:intergenic 45.0%
TTACCTCCAACCAACACAAC+AGG + chr7.3:72048635-72048654 None:intergenic 45.0%
TTCAACTCCTCCAATCCAGA+TGG + chr7.3:72048359-72048378 None:intergenic 45.0%
TTCACTTGCGGAGTTTGATG+AGG - chr7.3:72049072-72049091 MS.gene001948:intron 45.0%
TTGCAATGGGATGCTGCAAA+TGG - chr7.3:72050798-72050817 MS.gene001948:CDS 45.0%
TTGTTAACATCGCCGGACTT+AGG + chr7.3:72050457-72050476 None:intergenic 45.0%
! GGACCGGTATTTGTTCTAGA+TGG - chr7.3:72050757-72050776 MS.gene001948:CDS 45.0%
! GGGATTGGAAAGAGTTTGCT+TGG - chr7.3:72048330-72048349 MS.gene001948:CDS 45.0%
!! TGCTGCTTTGTTAACATCGC+CGG + chr7.3:72050464-72050483 None:intergenic 45.0%
ATGAAGAGAGAAGGTGGCTG+AGG + chr7.3:72049151-72049170 None:intergenic 50.0%
CAACCCGAGTCAAAAGATCC+GGG + chr7.3:72050577-72050596 None:intergenic 50.0%
CGGCTTATCTTTCGCCTTTC+CGG + chr7.3:72050734-72050753 None:intergenic 50.0%
GCAGCATCCCATTGCAATGA+AGG + chr7.3:72050795-72050814 None:intergenic 50.0%
GGACCTAGAATCCTGCACTT+TGG - chr7.3:72050277-72050296 MS.gene001948:intron 50.0%
GGTAGGAAGCTCTCTTCTGA+TGG - chr7.3:72050547-72050566 MS.gene001948:CDS 50.0%
GGTTCATCCGCAGGTGAAAA+AGG - chr7.3:72050523-72050542 MS.gene001948:CDS 50.0%
GTCAACACATCCGTGACTGT+TGG + chr7.3:72050068-72050087 None:intergenic 50.0%
TCAGCAGCCATGAAGAGAGA+AGG + chr7.3:72049160-72049179 None:intergenic 50.0%
TCAGCCACCTTCTCTCTTCA+TGG - chr7.3:72049150-72049169 MS.gene001948:intron 50.0%
TCATCCGCAGGTGAAAAAGG+TGG - chr7.3:72050526-72050545 MS.gene001948:CDS 50.0%
TGCTTGGGATCCATCTGGAT+TGG - chr7.3:72048346-72048365 MS.gene001948:CDS 50.0%
TTCACCTGCGGATGAACCAA+CGG + chr7.3:72050521-72050540 None:intergenic 50.0%
! GAGTTTGCTTGGGATCCATC+TGG - chr7.3:72048341-72048360 MS.gene001948:CDS 50.0%
! TTGGGATCCATCTGGATTGG+AGG - chr7.3:72048349-72048368 MS.gene001948:CDS 50.0%
!! AAGCCCGGATCTTTTGACTC+GGG - chr7.3:72050571-72050590 MS.gene001948:CDS 50.0%
!! ACGAAGGAGTACTCGTACTC+CGG - chr7.3:72050712-72050731 MS.gene001948:CDS 50.0%
!! ACTCCTGTTGTGTTGGTTGG+AGG - chr7.3:72048629-72048648 MS.gene001948:CDS 50.0%
!! CAAGCCCGGATCTTTTGACT+CGG - chr7.3:72050570-72050589 MS.gene001948:CDS 50.0%
!! CCTACCACCTTTTTCACCTG+CGG + chr7.3:72050533-72050552 None:intergenic 50.0%
!! GGATTTGAGGCTTGGTGATC+AGG - chr7.3:72048397-72048416 MS.gene001948:CDS 50.0%
CCGCAGGTGAAAAAGGTGGT+AGG - chr7.3:72050530-72050549 MS.gene001948:CDS 55.0%
CTCTCTTCTGATGGCAAGCC+CGG - chr7.3:72050556-72050575 MS.gene001948:CDS 55.0%
GCAGCCATGAAGAGAGAAGG+TGG + chr7.3:72049157-72049176 None:intergenic 55.0%
GGATGGCTGATAGGAGTTCC+AGG + chr7.3:72050487-72050506 None:intergenic 55.0%
GGTTCTGCCAACAATGCAGC+AGG - chr7.3:72048663-72048682 MS.gene001948:CDS 55.0%
TCTGAGGGCTACCAAAGTGC+AGG + chr7.3:72050291-72050310 None:intergenic 55.0%
TGGTTGGAGGTAAGCAGCAG+AGG - chr7.3:72048642-72048661 MS.gene001948:CDS 55.0%
! GGAGTACTCGTACTCCGGAA+AGG - chr7.3:72050717-72050736 MS.gene001948:CDS 55.0%
CCTATCAGCCATCCCATCCG+CGG - chr7.3:72050493-72050512 MS.gene001948:intron 60.0%
CCGCGGATGGGATGGCTGAT+AGG + chr7.3:72050496-72050515 None:intergenic 65.0%
CGGATGAACCAACGGCACCG+CGG + chr7.3:72050513-72050532 None:intergenic 65.0%
GAACCAACGGCACCGCGGAT+GGG + chr7.3:72050508-72050527 None:intergenic 65.0%
TGAACCAACGGCACCGCGGA+TGG + chr7.3:72050509-72050528 None:intergenic 65.0%
!! GGTGCCGTTGGTTCATCCGC+AGG - chr7.3:72050514-72050533 MS.gene001948:intron 65.0%
CAACGGCACCGCGGATGGGA+TGG + chr7.3:72050504-72050523 None:intergenic 70.0%
!! CATCCCATCCGCGGTGCCGT+TGG - chr7.3:72050502-72050521 MS.gene001948:intron 70.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 72048323 72050879 72048323 ID=MS.gene001948
chr7.3 mRNA 72048323 72050879 72048323 ID=MS.gene001948.t1;Parent=MS.gene001948
chr7.3 exon 72050518 72050879 72050518 ID=MS.gene001948.t1.exon1;Parent=MS.gene001948.t1
chr7.3 CDS 72050518 72050879 72050518 ID=cds.MS.gene001948.t1;Parent=MS.gene001948.t1
chr7.3 exon 72050000 72050136 72050000 ID=MS.gene001948.t1.exon2;Parent=MS.gene001948.t1
chr7.3 CDS 72050000 72050136 72050000 ID=cds.MS.gene001948.t1;Parent=MS.gene001948.t1
chr7.3 exon 72048323 72048924 72048323 ID=MS.gene001948.t1.exon3;Parent=MS.gene001948.t1
chr7.3 CDS 72048323 72048924 72048323 ID=cds.MS.gene001948.t1;Parent=MS.gene001948.t1
Gene Sequence

>MS.gene001948

ATGGATTGGGATTGGAAAGAGTTTGCTTGGGATCCATCTGGATTGGAGGAGTTGAAAAATGAAGGTGATTCAATGGATTTGAGGCTTGGTGATCAGGCTTCTGATTCATTGGAGAAATCAGTTCATGATTCAGGAAAGGAAGATTCAAAAGCTGTGTCGTCGTCGTCACCTACTAGATCGTTGAAAAGAGCGCGTTTGCAAAATGGATCGCAGAATATGATTTGCTCGGTTGATGGATGTAACTCTGATCTTAGTGATTGTAGAGAGTATCATAAGCGTCATAGGGTGTGCGAAAAGCATTCTAAAACTCCTGTTGTGTTGGTTGGAGGTAAGCAGCAGAGGTTCTGCCAACAATGCAGCAGGTATATGAATTGAAGTTGTTTGTTTCGGGTTGATAACTTGTTGAATTTTGTTGTTTTAGTGTCATGTTTGAAGAAACAGCTTAATTAAACTCTGATAGCATTATTGTTTATCATTATAAGTGTTTATAATTTATGTATCTATGTGCACGTTATTTCTATAACAGTATAAAATAAAGTCAAACTGTTTTCATAAGCTATTCTGCAAAGAATATGAAAATGAGATGAAAACAACTTATGGACATGTCATAAGCTGTTTCCATATATAAGCTCTCTGAATATTCTAAAAATGGCTTATTTGAGTAGATAAGCTTAAAAATAACAATCCAAACATACCGAGTTTTGAGGTTGTTTTGTAATATGGGGTGGTTCAATTTTATGTAGGTTTCATTCACTTGCGGAGTTTGATGAGGTTAAGAGGAGTTGCAGGAAACGTCTTGATGGACATAATAAGCGCAGGAGGAAACCTCAGCCACCTTCTCTCTTCATGGCTGCTGAGAAGTTCATGTACAATTACAAAGGTTTGTGTCTTTTGTATCTCATGATTCTAAGATAGATGGCTTATTTCTGTCGATGTTTAACAAGATCTCAGTTTTATGATTGTCAAACCTGAGATGAAAGAGGGAATTATGACGAAGATATAACGTTTTTTATGGAAGAAGATACGCATGATGAATAAGTAAGAAATCACATATACATAACAATCCATAAGGTTTTGACAATAGAATTTAGAGTATCTCTCAAAATTTGTTTCAAATTTTTATTTACCATGAACTGAATTCTGAGCTAAGCTCAATAATTAGCTATAGTTATTTAGCGACTTTTGGATATACAACTTAATTAAAAACATACTTTATAAGTGCTTATTGTGAAAGAGTTTATGTTTAAGCTGATTCTACTATTGAAGAGGGAATGCATAAAAACTCTGGGTAAAGCTAAAATTAGCTTAGTGCAATAAGTTTCTAGGTGATGCTTAGCAAATATGTTAAAAGCAACTTAACAAACATGTCGTAATCTATTTTCATTTGCTTTTCCAAATACTTGCGCAAGTGTTTATGTCATAAGATAAGTTTAAATAAGCGCAAGTGTTTATGTCATAAGATAAGTTTAAATAAGCTCTTCCAATCCTCTCTCTAATCAAATTTAATGACAAAGAAAGTGACCTTCCGTCTTTATTTTCACAAGAAAGATGGTGGAGAAATATGAAACTTTGCTAATATTTCCCCATGGATTTCAATTGCAAAAAATGGATGCCCTGCATTTACACGATTAGTGCTTTAGTAACTGTTGGATTTAGATCGGACATTTTACAAAATTTGTTGATTTTGATCTTTTCGAAAATTCAAAATACTGTGCTGAAAATTCGCACGGTCCAATCTTGATCCAACAGTCACGGATGTGTTGACTGCGTGAATTCATAATATCCCAACTGCAGAGAATCTTGATCCTTGATTTCCGGCATGTTCAAACTTAACTAATATGCTTACTGCTGACATCTGTTTTTTTTTCTAGTCATAATTAATTACATTATTTTTGTCTTCCACAAGCACAGGTAATATGGAGTTTTAACACTGTTTTCTCTTTGTACAATTTTAGGACCTAGAATCCTGCACTTTGGTAGCCCTCAGACATATGTCAATCCTATTATGAGAAACATTTGGCCTGCAACTTCCATAACTGAAGCTGAATCTGGTTATGATCATCAAAGACTTTTGTATCGAATCGACAAACATAAACAGGATAAGGGACATCCTCATTGGCAAGAAATTCTCCCTAAGTCCGGCGATGTTAACAAAGCAGCACCTGGAACTCCTATCAGCCATCCCATCCGCGGTGCCGTTGGTTCATCCGCAGGTGAAAAAGGTGGTAGGAAGCTCTCTTCTGATGGCAAGCCCGGATCTTTTGACTCGGGTTGTGCTCTCTATCTTCTGTCAACACTTCAAACACAAAGCTCAGAGTTGAGTTTGATGCAGTCAAGCATCAACAACAGTCCAACACAATCTTCGTCTGGAACGTTGCATTTTGATGCTACGAAGGAGTACTCGTACTCCGGAAAGGCGAAAGATAAGCCGAATGGACCGGTATTTGTTCTAGATGGAAACACAACAAACCTTCATTGCAATGGGATGCTGCAAATGGGTCCTAATGGATTGATCGAAAATGATAACTCAGTGACTCTTCCATTCTTCTGGGAGTAG
Protein sequence

>MS.gene001948.t1

MDWDWKEFAWDPSGLEELKNEGDSMDLRLGDQASDSLEKSVHDSGKEDSKAVSSSSPTRSLKRARLQNGSQNMICSVDGCNSDLSDCREYHKRHRVCEKHSKTPVVLVGGKQQRFCQQCSRFHSLAEFDEVKRSCRKRLDGHNKRRRKPQPPSLFMAAEKFMYNYKGPRILHFGSPQTYVNPIMRNIWPATSITEAESGYDHQRLLYRIDKHKQDKGHPHWQEILPKSGDVNKAAPGTPISHPIRGAVGSSAGEKGGRKLSSDGKPGSFDSGCALYLLSTLQTQSSELSLMQSSINNSPTQSSSGTLHFDATKEYSYSGKAKDKPNGPVFVLDGNTTNLHCNGMLQMGPNGLIENDNSVTLPFFWE