AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene001953

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001953.t1 MTR_8g071030 86.417 773 73 3 1 765 1 749 0.0 1310
MS.gene001953.t1 MTR_8g070070 74.520 781 171 8 1 765 1 769 0.0 1145
MS.gene001953.t1 MTR_8g070075 64.713 785 223 9 1 761 4 758 0.0 1009
MS.gene001953.t1 MTR_1g035420 63.197 538 183 6 229 764 134 658 0.0 697
MS.gene001953.t1 MTR_1g035400 61.702 470 177 3 288 756 101 568 0.0 608
MS.gene001953.t1 MTR_6g044690 57.419 465 175 8 298 753 114 564 0.0 538
MS.gene001953.t1 MTR_0376s0030 51.176 510 222 8 267 764 83 577 4.55e-172 507
MS.gene001953.t1 MTR_6g043960 51.509 464 183 8 298 753 53 482 1.60e-157 466
MS.gene001953.t1 MTR_2g054460 44.112 569 201 13 194 753 4 464 4.51e-144 431
MS.gene001953.t1 MTR_7g088370 43.312 471 225 17 296 753 239 680 6.41e-107 342
MS.gene001953.t1 MTR_5g013420 40.000 460 253 8 296 751 618 1058 1.98e-105 348
MS.gene001953.t1 MTR_5g013420 40.000 460 253 8 296 751 618 1058 2.17e-105 348
MS.gene001953.t1 MTR_3g011440 36.315 559 321 13 204 752 100 633 1.47e-104 335
MS.gene001953.t1 MTR_4g010830 40.741 459 250 9 296 751 510 949 3.80e-102 337
MS.gene001953.t1 MTR_8g042750 40.381 473 249 13 296 753 204 658 9.57e-102 328
MS.gene001953.t1 MTR_4g010550 38.961 462 224 12 296 751 24 433 1.49e-93 300
MS.gene001953.t1 MTR_7g084090 37.967 482 259 14 296 752 261 727 1.20e-87 293
MS.gene001953.t1 MTR_6g061270 37.838 481 260 13 296 752 199 664 2.23e-86 288
MS.gene001953.t1 MTR_6g061200 37.421 473 244 15 296 752 199 635 1.27e-80 271
MS.gene001953.t1 MTR_7g084090 41.322 363 183 11 296 641 261 610 3.50e-72 249
MS.gene001953.t1 MTR_7g445710 31.875 480 228 17 295 751 109 512 5.23e-54 196
MS.gene001953.t1 MTR_7g445530 31.667 480 229 17 295 751 109 512 7.28e-54 194
MS.gene001953.t1 MTR_1g048950 32.979 470 264 15 298 751 73 507 4.36e-52 191
MS.gene001953.t1 MTR_7g450730 30.349 458 227 15 296 751 107 474 7.33e-51 186
MS.gene001953.t1 MTR_7g052480 31.613 310 186 9 459 752 227 526 1.13e-38 152
MS.gene001953.t1 MTR_7g445600 34.667 150 61 2 602 751 243 355 1.33e-22 100
MS.gene001953.t1 MTR_7g445600 37.984 129 66 5 296 424 106 220 4.64e-15 77.8
MS.gene001953.t1 MTR_6g044000 67.925 53 17 0 701 753 117 169 1.21e-20 90.1
MS.gene001953.t1 MTR_6g044000 52.941 68 32 0 298 365 53 120 2.47e-18 83.6
MS.gene001953.t1 MTR_8g078490 29.730 185 118 3 568 751 1056 1229 3.72e-20 96.7
MS.gene001953.t1 MTR_5g018850 31.492 181 112 3 568 747 1310 1479 4.26e-20 96.7
MS.gene001953.t1 MTR_8g078505 27.857 280 155 8 503 751 252 515 6.41e-20 94.7
MS.gene001953.t1 MTR_4g132610 29.101 189 112 3 569 751 851 1023 6.27e-19 92.4
MS.gene001953.t1 MTR_5g098680 36.076 158 87 4 321 473 267 415 4.90e-16 82.8
MS.gene001953.t1 MTR_8g102580 36.076 158 87 4 321 473 261 409 1.04e-15 81.6
MS.gene001953.t1 MTR_7g445810 28.287 251 130 10 226 450 38 264 6.28e-13 70.5
MS.gene001953.t1 MTR_3g114910 33.333 153 88 4 321 473 187 325 1.40e-12 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene001953.t1 AT4G13460 61.237 485 181 5 282 764 170 649 0.0 629
MS.gene001953.t1 AT4G13460 61.237 485 181 5 282 764 170 649 0.0 629
MS.gene001953.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene001953.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene001953.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene001953.t1 AT2G33290 58.264 484 190 5 282 764 178 650 0.0 602
MS.gene001953.t1 AT5G04940 40.166 483 259 15 279 753 180 640 2.54e-98 319
MS.gene001953.t1 AT5G04940 40.166 483 259 15 279 753 180 640 2.54e-98 319
MS.gene001953.t1 AT1G73100 36.347 553 312 15 220 753 108 639 8.98e-96 312
MS.gene001953.t1 AT2G22740 36.820 478 275 8 285 751 298 759 4.45e-93 308
MS.gene001953.t1 AT2G22740 36.820 478 275 8 285 751 298 759 4.45e-93 308
MS.gene001953.t1 AT2G35160 34.857 525 291 13 236 751 245 727 8.18e-90 298
MS.gene001953.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene001953.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene001953.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene001953.t1 AT2G35160 34.857 525 291 13 236 751 281 763 3.00e-89 298
MS.gene001953.t1 AT5G13960 36.831 486 258 16 296 752 129 594 1.20e-83 278
MS.gene001953.t1 AT5G13960 37.313 469 246 15 313 752 11 460 5.03e-83 273
MS.gene001953.t1 AT1G17770 31.836 512 314 16 258 753 169 661 6.26e-72 249
MS.gene001953.t1 AT2G24740 36.078 510 285 18 253 753 247 724 6.32e-72 250
MS.gene001953.t1 AT5G47150 40.449 178 97 4 296 473 156 324 7.37e-28 115
MS.gene001953.t1 AT5G47160 40.351 171 92 5 300 469 246 407 3.25e-24 106
MS.gene001953.t1 AT5G47160 40.351 171 92 5 300 469 246 407 3.25e-24 106
MS.gene001953.t1 AT5G47160 40.351 171 92 5 300 469 246 407 3.25e-24 106
MS.gene001953.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 5.34e-22 102
MS.gene001953.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 5.34e-22 102
MS.gene001953.t1 AT2G23740 30.964 197 109 5 570 756 1184 1363 5.34e-22 102
MS.gene001953.t1 AT2G23740 30.964 197 109 5 570 756 1177 1356 5.42e-22 102
MS.gene001953.t1 AT2G05900 38.506 174 99 6 358 531 26 191 1.28e-19 90.9
MS.gene001953.t1 AT1G57820 36.047 172 86 6 317 473 264 426 1.21e-16 84.7
MS.gene001953.t1 AT1G57820 36.047 172 86 6 317 473 264 426 1.21e-16 84.7
MS.gene001953.t1 AT3G03750 30.918 207 115 6 548 752 143 323 3.45e-16 81.3
MS.gene001953.t1 AT1G57820 35.503 169 88 6 317 473 264 423 1.52e-15 81.3
MS.gene001953.t1 AT5G39550 34.302 172 89 6 317 473 249 411 1.73e-15 80.9
MS.gene001953.t1 AT2G23740 30.405 148 79 4 570 710 1184 1314 5.16e-15 80.1
MS.gene001953.t1 AT2G23740 30.405 148 79 4 570 710 1184 1314 5.16e-15 80.1
MS.gene001953.t1 AT1G66040 31.977 172 93 5 317 473 249 411 1.07e-14 78.6
MS.gene001953.t1 AT1G66050 31.977 172 93 5 317 473 249 411 1.12e-14 78.2

Find 231 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAATCTCAACCTTAATT+TGG 0.115237 8.3:+31510450 MS.gene001953:CDS
GCTAGGGATATTAAGAAAAT+TGG 0.127502 8.3:+31511486 MS.gene001953:CDS
CCAAATTAAGGTTGAGATTT+AGG 0.131281 8.3:-31510450 None:intergenic
GCTACTAGTGTAATTGTTTC+AGG 0.143034 8.3:+31511120 MS.gene001953:CDS
CCCCATTCTACCCATCTATT+TGG 0.166114 8.3:-31512029 None:intergenic
CTATTGATATTTCAAATAAT+AGG 0.206413 8.3:+31511538 MS.gene001953:CDS
GCAAATTTGTGATCAGAAAT+TGG 0.219259 8.3:+31511227 MS.gene001953:CDS
ACATACTGAATGAATATATT+TGG 0.228005 8.3:-31512176 None:intergenic
GATGGATATGGTGGTGATTT+GGG 0.254601 8.3:-31510191 None:intergenic
AAGCAATCCATGTGGATTAT+AGG 0.255997 8.3:-31511748 None:intergenic
TAGGTCCTTGGACCTTATTC+AGG 0.261644 8.3:+31511905 MS.gene001953:CDS
CGATGGATATGGTGGTGATT+TGG 0.266979 8.3:-31510192 None:intergenic
CTAAATCTCAACCTTAATTT+GGG 0.267648 8.3:+31510451 MS.gene001953:CDS
GGTGAGAGCGGTGTGATTTA+AGG 0.267982 8.3:-31510164 None:intergenic
TCAGATTCGGGAGAGAAATT+AGG 0.279456 8.3:-31510295 None:intergenic
TGATTTAAGGTTGAAATTCT+AGG 0.282052 8.3:-31510151 None:intergenic
TGAGAGTGGGTGAGGAGAAT+AGG 0.282380 8.3:+31510824 MS.gene001953:CDS
TAGGTTGTTGTTGGGAATCT+TGG 0.291847 8.3:-31510276 None:intergenic
GAGAGTGGGTGAGGAGAATA+GGG 0.304281 8.3:+31510825 MS.gene001953:CDS
AATTGGTTGTTGAAGTTGAT+AGG 0.309699 8.3:+31510716 MS.gene001953:CDS
TATTGATATTTCAAATAATA+GGG 0.311494 8.3:+31511539 MS.gene001953:CDS
TGATCATCGGTTTCAGATTC+GGG 0.313526 8.3:-31510307 None:intergenic
GCAACACGGTTTCGACAATT+AGG 0.314745 8.3:-31511822 None:intergenic
GATATAGGAAAGTCTGGTTT+TGG 0.315495 8.3:+31511396 MS.gene001953:CDS
GTCGAGTTTGATGGCATATA+TGG 0.315529 8.3:+31510534 MS.gene001953:CDS
GAATGGGTTTCAAGGGTTAA+TGG 0.316851 8.3:-31510049 None:intergenic
TTGATCATCGGTTTCAGATT+CGG 0.324605 8.3:-31510308 None:intergenic
AATATAGCTTGGAAGTGTTT+AGG 0.327076 8.3:+31511859 MS.gene001953:CDS
AATGAATGAGGAGAAGAAAT+TGG 0.327732 8.3:+31510699 MS.gene001953:CDS
AGCAATCCATGTGGATTATA+GGG 0.332580 8.3:-31511747 None:intergenic
GACGCGAAGAAATTGTTAAA+AGG 0.332988 8.3:-31510253 None:intergenic
ATATATTTGGACTCGAACTA+TGG 0.334818 8.3:-31512163 None:intergenic
ATCTTGCTCGCCCACTCGTC+CGG 0.340319 8.3:-31512296 None:intergenic
TTAGGAGGACATGAGCAAAA+AGG 0.343616 8.3:-31511804 None:intergenic
AGAGAACTCTGCATAGATTA+TGG 0.345054 8.3:+31512269 MS.gene001953:CDS
AATGGGTTTCAAGGGTTAAT+GGG 0.348241 8.3:-31510048 None:intergenic
GGGAGACAATTAATGGAATA+AGG 0.348461 8.3:+31510869 MS.gene001953:CDS
ATATCATTAAATAGCCTAAC+TGG 0.350719 8.3:-31511570 None:intergenic
GTTAGTTGCTTGTTTGATAT+AGG 0.351027 8.3:+31511381 MS.gene001953:CDS
AGAAATTGTTAAAAGGTTCC+AGG 0.355471 8.3:-31510246 None:intergenic
CACAGACAGGTTGGGGAGTT+AGG 0.355710 8.3:+31511886 MS.gene001953:CDS
ATTGGATGAGGAGAAGATAT+TGG 0.356889 8.3:+31510771 MS.gene001953:CDS
CTAGTACATTTCCAAATAGA+TGG 0.359463 8.3:+31512018 MS.gene001953:CDS
GAAGGGAGTGCATCTACTTC+TGG 0.362995 8.3:+31511324 MS.gene001953:CDS
TAAAGGATCCAGCCTGAATA+AGG 0.368631 8.3:-31511917 None:intergenic
TTTGATGGCATATATGGTTA+TGG 0.371504 8.3:+31510540 MS.gene001953:CDS
ATTGTCTGAGGAAAGGAAAT+TGG 0.371576 8.3:+31510753 MS.gene001953:CDS
AAGTGATGTTCCGATGGATA+TGG 0.384008 8.3:-31510203 None:intergenic
ACCAGTATACTCGCAGATAA+AGG 0.385474 8.3:-31511934 None:intergenic
TATGGTGGTGATTTGGGGTG+GGG 0.386266 8.3:-31510185 None:intergenic
TTGTTAAAAGGTTCCAGGTT+TGG 0.388510 8.3:-31510241 None:intergenic
CGTAGTATCAACCACATTCA+TGG 0.388913 8.3:+31510895 MS.gene001953:CDS
CTAGGGATATTAAGAAAATT+GGG 0.394850 8.3:+31511487 MS.gene001953:CDS
TCGAAACCGTGTTGCACAAA+AGG 0.395320 8.3:+31511830 MS.gene001953:CDS
TGTTGTTTCATTGGAGAATA+CGG 0.399431 8.3:+31510408 MS.gene001953:CDS
TCCAAATAGATGGGTAGAAT+GGG 0.401426 8.3:+31512028 MS.gene001953:CDS
GAGAGAAATTAGGTTGTTGT+TGG 0.401584 8.3:-31510285 None:intergenic
TGCACTATGGGATTGAGGTT+AGG 0.405470 8.3:+31511280 MS.gene001953:CDS
ACCACATTCATGGTTATATG+AGG 0.405787 8.3:+31510905 MS.gene001953:CDS
TCAAAGAGTATGTCTTATAA+TGG 0.405858 8.3:+31511087 MS.gene001953:CDS
GATATGGTGGTGATTTGGGG+TGG 0.408728 8.3:-31510187 None:intergenic
TAGAATTTGGCGTATAGGTT+GGG 0.409698 8.3:-31510331 None:intergenic
TGTGGTAGAATTTGGCGTAT+AGG 0.411852 8.3:-31510336 None:intergenic
AGAAATAGAAGTAGAAGAAT+GGG 0.413089 8.3:-31510065 None:intergenic
GCACTATGGGATTGAGGTTA+GGG 0.416487 8.3:+31511281 MS.gene001953:CDS
TTCCAAATAGATGGGTAGAA+TGG 0.421060 8.3:+31512027 MS.gene001953:CDS
TTGTTTGATATAGGAAAGTC+TGG 0.421956 8.3:+31511390 MS.gene001953:CDS
TAGGTCTACACAGACAGGTT+GGG 0.422135 8.3:+31511878 MS.gene001953:CDS
GAGAAATAGAAGTAGAAGAA+TGG 0.422660 8.3:-31510066 None:intergenic
TGAAACAACAAAATCATTGT+TGG 0.424236 8.3:-31510395 None:intergenic
GGCAGAGATGAGAGTGGGTG+AGG 0.425042 8.3:+31510816 MS.gene001953:CDS
TCCTTTATCTGCGAGTATAC+TGG 0.426481 8.3:+31511933 MS.gene001953:CDS
AGGTTGTTGTTGGGAATCTT+GGG 0.427326 8.3:-31510275 None:intergenic
CATCTATTTGGAAATGTACT+AGG 0.428903 8.3:-31512017 None:intergenic
GAAACAAGAGTCGAGTTTGA+TGG 0.434269 8.3:+31510525 MS.gene001953:CDS
AAGTAGAAGAATGGGTTTCA+AGG 0.435832 8.3:-31510057 None:intergenic
TATAAGTTATTGAGAATTGA+GGG 0.436671 8.3:+31511429 MS.gene001953:CDS
GTAGAATTTGGCGTATAGGT+TGG 0.436679 8.3:-31510332 None:intergenic
TCTTTATTATGAGTATCTTC+CGG 0.438277 8.3:+31511611 MS.gene001953:CDS
ATTGGTTGTTGAAGTTGATA+GGG 0.446123 8.3:+31510717 MS.gene001953:CDS
TCCTTGGACCTTATTCAGGC+TGG 0.446886 8.3:+31511909 MS.gene001953:CDS
CGAAACCGTGTTGCACAAAA+GGG 0.448108 8.3:+31511831 MS.gene001953:CDS
AATTGAGGGGCAGGCGAAGA+TGG 0.448823 8.3:+31511443 MS.gene001953:CDS
AAACTCGACTCTTGTTTCTC+AGG 0.449003 8.3:-31510520 None:intergenic
AGGAGGGAATTGTGTGTGAC+TGG 0.450008 8.3:+31511027 MS.gene001953:CDS
ACCTAGTGGTTGTGAATGTA+AGG 0.454638 8.3:+31511674 MS.gene001953:CDS
GGAATAGGAACATGAGAAGA+AGG 0.457689 8.3:-31510091 None:intergenic
AGAGAAATTAGGTTGTTGTT+GGG 0.464968 8.3:-31510284 None:intergenic
ATATGGTGGTGATTTGGGGT+GGG 0.466003 8.3:-31510186 None:intergenic
TTGATGAGAAATACTAAGAT+CGG 0.467844 8.3:-31510125 None:intergenic
GATTTAAGGTTGAAATTCTA+GGG 0.470586 8.3:-31510150 None:intergenic
TAAGGTTGAGATTTAGGTCC+GGG 0.474895 8.3:-31510444 None:intergenic
TTGGAAAGGAGTATGCACTA+TGG 0.475190 8.3:+31511267 MS.gene001953:CDS
ACATCACCAACATAAATTCC+AGG 0.479414 8.3:-31510994 None:intergenic
TGATGAGAAATACTAAGATC+GGG 0.480139 8.3:-31510124 None:intergenic
GGTTTGTGTTTCATCAGAGT+GGG 0.481554 8.3:+31511649 MS.gene001953:CDS
AGTAGAAGAATGGGTTTCAA+GGG 0.482906 8.3:-31510056 None:intergenic
GGATATTAAGAAAATTGGGT+TGG 0.483689 8.3:+31511491 MS.gene001953:CDS
AGGATAGTTGGACCGATACC+TGG 0.485997 8.3:+31510976 MS.gene001953:CDS
TTCGCTTCGCGTGAGAATTG+AGG 0.488587 8.3:+31510666 MS.gene001953:CDS
TCTTGCTCGCCCACTCGTCC+GGG 0.490402 8.3:-31512295 None:intergenic
GGTTATGGAGAAGGAAAGCC+AGG 0.494082 8.3:+31510555 MS.gene001953:CDS
AATTGTTTCAGGTGGTTATG+AGG 0.495448 8.3:+31511131 MS.gene001953:CDS
TAATCTAAAGGAGGTATTGA+CGG 0.498715 8.3:-31512095 None:intergenic
ATAGGGAAAATGTTCCAGTT+AGG 0.499113 8.3:+31511556 MS.gene001953:CDS
TATCGTGATCAAAGGATAGT+TGG 0.501111 8.3:+31510964 MS.gene001953:CDS
GTATAAGTTATTGAGAATTG+AGG 0.501728 8.3:+31511428 MS.gene001953:CDS
CACTGTTCTCAAAGATGCTA+GGG 0.502270 8.3:+31511470 MS.gene001953:CDS
CCCTCACAAGTTCCATCACC+TGG 0.503770 8.3:-31510573 None:intergenic
TCATGTCATGTTATATGCAA+TGG 0.506033 8.3:+31512229 MS.gene001953:CDS
TCTATGCAGAGTTCTCTCAT+AGG 0.506224 8.3:-31512263 None:intergenic
GTCGGCTACCAACGTGGAGG+GGG 0.510887 8.3:+31510849 MS.gene001953:CDS
AAAGGGGCTCAAATATAGCT+TGG 0.511664 8.3:+31511848 MS.gene001953:CDS
GAATCAGCGATTTCGAGAAA+AGG 0.512004 8.3:-31510373 None:intergenic
AGGTCTACACAGACAGGTTG+GGG 0.513098 8.3:+31511879 MS.gene001953:CDS
GATTTGGGGTGGGGTGAGAG+CGG 0.514925 8.3:-31510176 None:intergenic
GGGGTGGATTCTGTAAATGA+TGG 0.515279 8.3:+31510472 MS.gene001953:CDS
ACTCGAACTATGGCTCATAT+AGG 0.515301 8.3:-31512153 None:intergenic
TTAAGGTTGAGATTTAGGTC+CGG 0.515596 8.3:-31510445 None:intergenic
GAGTGCATCTACTTCTGGTA+AGG 0.518621 8.3:+31511329 MS.gene001953:CDS
GTATAGGTTGGGTTGATCAT+CGG 0.521132 8.3:-31510320 None:intergenic
TCAATAGAGATACAACTCAT+TGG 0.521177 8.3:-31511522 None:intergenic
TGCACTTTCGTCATAAGATA+AGG 0.521451 8.3:+31510623 MS.gene001953:CDS
GTGTCGGCTACCAACGTGGA+GGG 0.521469 8.3:+31510847 MS.gene001953:CDS
GCACTGTTCTCAAAGATGCT+AGG 0.522783 8.3:+31511469 MS.gene001953:CDS
TGTCGGCTACCAACGTGGAG+GGG 0.522877 8.3:+31510848 MS.gene001953:CDS
GCATACTCCTTTCCAATGCA+AGG 0.525232 8.3:-31511260 None:intergenic
GGCATATATGGTTATGGAGA+AGG 0.527217 8.3:+31510546 MS.gene001953:CDS
GGAGGTATTGACGGATAAGA+TGG 0.528649 8.3:-31512086 None:intergenic
TAATGAAGGAGCGTGGGCTA+TGG 0.529339 8.3:+31510938 MS.gene001953:CDS
AATCAGCGATTTCGAGAAAA+GGG 0.530354 8.3:-31510372 None:intergenic
AGAAGAGATTGTCTGAGGAA+AGG 0.532258 8.3:+31510746 MS.gene001953:CDS
GGAGAAGGAAAGCCAGGTGA+TGG 0.532924 8.3:+31510561 MS.gene001953:CDS
TGGTTTGTGTTTCATCAGAG+TGG 0.533225 8.3:+31511648 MS.gene001953:CDS
ATTATAATCTACACTGGCCA+TGG 0.534081 8.3:+31511180 MS.gene001953:CDS
TACTGGTGTTGCTATAACTA+GGG 0.536645 8.3:+31511950 MS.gene001953:CDS
AACAATGAATGGGGTCTCGT+TGG 0.538408 8.3:+31511989 MS.gene001953:CDS
GTTATTGAGAATTGAGGGGC+AGG 0.539794 8.3:+31511434 MS.gene001953:CDS
ACAATTACACTAGTAGCAAT+CGG 0.540891 8.3:-31511114 None:intergenic
TGGAAAGGAGTATGCACTAT+GGG 0.541585 8.3:+31511268 MS.gene001953:CDS
AATTCTTCTCTCGCCCTCCA+TGG 0.544307 8.3:-31511197 None:intergenic
GGTTGTTGAAGTTGATAGGG+AGG 0.545333 8.3:+31510720 MS.gene001953:CDS
ACATCCAGAAAATAATCTAA+AGG 0.546485 8.3:-31512107 None:intergenic
CTGATCACAAATTTGCCTTG+AGG 0.547153 8.3:-31511220 None:intergenic
ATTGAGGGGCAGGCGAAGAT+GGG 0.548419 8.3:+31511444 MS.gene001953:CDS
ATACTGGTGTTGCTATAACT+AGG 0.549242 8.3:+31511949 MS.gene001953:CDS
AACGCCACTCATTCCAAACC+TGG 0.552250 8.3:+31510228 MS.gene001953:CDS
GAGTATGCACTATGGGATTG+AGG 0.552471 8.3:+31511275 MS.gene001953:CDS
CCAGGTGATGGAACTTGTGA+GGG 0.555665 8.3:+31510573 MS.gene001953:CDS
ATGCAGAGTTCTCTCATAGG+AGG 0.558353 8.3:-31512260 None:intergenic
GGAGGGTGGAAACCTTGCAT+TGG 0.558530 8.3:+31511248 MS.gene001953:CDS
CTCTGATGAAACACAAACCA+CGG 0.558774 8.3:-31511645 None:intergenic
GGAAGATACTCATAATAAAG+AGG 0.560347 8.3:-31511609 None:intergenic
GGTAAGGTTTATGTTTACGA+TGG 0.560574 8.3:+31511345 MS.gene001953:CDS
GTTAATGGGAAAGGATCCAT+TGG 0.561622 8.3:-31510034 None:intergenic
TGAGGAAAGGAAATTGGATG+AGG 0.567529 8.3:+31510759 MS.gene001953:CDS
ATTCGCGGGATGAAGTATGA+AGG 0.568414 8.3:+31511306 MS.gene001953:CDS
GGAGGACAAGAGAATGAATG+AGG 0.571585 8.3:+31510687 MS.gene001953:CDS
GGAAGAGAAGAGATTGTCTG+AGG 0.572868 8.3:+31510741 MS.gene001953:CDS
AACCACGGGAAAGTAGTATC+CGG 0.574690 8.3:-31511630 None:intergenic
CCGATACCTGGAATTTATGT+TGG 0.577931 8.3:+31510988 MS.gene001953:CDS
ATTGAGGTTAGGGTTATTCG+CGG 0.577972 8.3:+31511291 MS.gene001953:CDS
GGTTCCAGGTTTGGAATGAG+TGG 0.578526 8.3:-31510232 None:intergenic
GGTTTCAGATCTGAGTATGA+AGG 0.579176 8.3:+31510594 MS.gene001953:CDS
GTGGAAACCTTGCATTGGAA+AGG 0.581268 8.3:+31511253 MS.gene001953:CDS
ATGGATATGGTGGTGATTTG+GGG 0.583200 8.3:-31510190 None:intergenic
CGACATTAATGAAGGAGCGT+GGG 0.584881 8.3:+31510932 MS.gene001953:CDS
TAGTACATTTCCAAATAGAT+GGG 0.585798 8.3:+31512019 MS.gene001953:CDS
GAGGGCTGCGACATTAATGA+AGG 0.586703 8.3:+31510924 MS.gene001953:CDS
CTGCATAGATTATGGCATCC+CGG 0.587677 8.3:+31512277 MS.gene001953:CDS
GGATGCAGAAGTTGCAGTTG+TGG 0.588062 8.3:+31510792 MS.gene001953:CDS
CCAACATAAATTCCAGGTAT+CGG 0.588388 8.3:-31510988 None:intergenic
TAAATCTCAACCTTAATTTG+GGG 0.590545 8.3:+31510452 MS.gene001953:CDS
GTGTTTAGGTCTACACAGAC+AGG 0.591190 8.3:+31511873 MS.gene001953:CDS
TATGGTTGTATCGTGATCAA+AGG 0.592056 8.3:+31510956 MS.gene001953:CDS
TGTGATCAGAAATTGGAGGG+TGG 0.592511 8.3:+31511234 MS.gene001953:CDS
GTTTCAAGGGTTAATGGGAA+AGG 0.597617 8.3:-31510043 None:intergenic
GCCAGGTGATGGAACTTGTG+AGG 0.598287 8.3:+31510572 MS.gene001953:CDS
TGCAGAGTTCTCTCATAGGA+GGG 0.599457 8.3:-31512259 None:intergenic
TAATCTACACTGGCCATGGA+GGG 0.600090 8.3:+31511184 MS.gene001953:CDS
ACACGGTTTCGACAATTAGG+AGG 0.600465 8.3:-31511819 None:intergenic
ACTAGTGTAATTGTTTCAGG+TGG 0.601921 8.3:+31511123 MS.gene001953:CDS
TACGGTAAATGTTTGCAACC+CGG 0.602140 8.3:+31510426 MS.gene001953:CDS
AGGTTGGGGAGTTAGGTCCT+TGG 0.602845 8.3:+31511893 MS.gene001953:CDS
AGGTGGTTATGAGGATGACA+TGG 0.604358 8.3:+31511140 MS.gene001953:CDS
GGTGATATTATAATCTACAC+TGG 0.606082 8.3:+31511174 MS.gene001953:CDS
GTGGAGGCAGAGATGAGAGT+GGG 0.608465 8.3:+31510811 MS.gene001953:CDS
CCACATTCATGGTTATATGA+GGG 0.608512 8.3:+31510906 MS.gene001953:CDS
TTCGTCATAAGATAAGGAAG+AGG 0.608918 8.3:+31510629 MS.gene001953:CDS
ATTGCATATAACATGACATG+AGG 0.609949 8.3:-31512227 None:intergenic
GCGACATTAATGAAGGAGCG+TGG 0.612288 8.3:+31510931 MS.gene001953:CDS
ATTATGGCATCCCGGACGAG+TGG 0.613316 8.3:+31512285 MS.gene001953:CDS
TCTGATGAAACACAAACCAC+GGG 0.613935 8.3:-31511644 None:intergenic
ATTTGTGATCAGAAATTGGA+GGG 0.614502 8.3:+31511231 MS.gene001953:CDS
TTGCATATGCATATGCAAGC+TGG 0.615698 8.3:+31511051 MS.gene001953:CDS
CGTTTGAAGTGATGTTCCGA+TGG 0.617924 8.3:-31510209 None:intergenic
GTTTGTGTTTCATCAGAGTG+GGG 0.619286 8.3:+31511650 MS.gene001953:CDS
GAGTTTCCCTATAATCCACA+TGG 0.620559 8.3:+31511741 MS.gene001953:CDS
CCCTCATATAACCATGAATG+TGG 0.621384 8.3:-31510906 None:intergenic
GGGTGAGGAGAATAGGGTGT+CGG 0.621767 8.3:+31510831 MS.gene001953:CDS
TCCAGCCTGAATAAGGTCCA+AGG 0.622985 8.3:-31511910 None:intergenic
ATAAGTTATTGAGAATTGAG+GGG 0.623555 8.3:+31511430 MS.gene001953:CDS
TTCGCGGGATGAAGTATGAA+GGG 0.624136 8.3:+31511307 MS.gene001953:CDS
TTATGGCATCCCGGACGAGT+GGG 0.624731 8.3:+31512286 MS.gene001953:CDS
CAAATCACCACCATATCCAT+CGG 0.625118 8.3:+31510193 MS.gene001953:CDS
ATAATCTACACTGGCCATGG+AGG 0.626398 8.3:+31511183 MS.gene001953:CDS
TTGAGGTTAGGGTTATTCGC+GGG 0.628964 8.3:+31511292 MS.gene001953:CDS
GGTGTCGGCTACCAACGTGG+AGG 0.632053 8.3:+31510846 MS.gene001953:CDS
TCCAGAAAATAATCTAAAGG+AGG 0.632885 8.3:-31512104 None:intergenic
TTAGGTCTACACAGACAGGT+TGG 0.633420 8.3:+31511877 MS.gene001953:CDS
TGTAAGGAGGCTTGTACTGA+CGG 0.637592 8.3:+31511690 MS.gene001953:CDS
TGCAGAAGTTGCAGTTGTGG+AGG 0.639345 8.3:+31510795 MS.gene001953:CDS
GATGAGAAATACTAAGATCG+GGG 0.640700 8.3:-31510123 None:intergenic
CCTTTCAAAAGCAATCCATG+TGG 0.644916 8.3:-31511756 None:intergenic
GCTTCGCGTGAGAATTGAGG+AGG 0.645275 8.3:+31510669 MS.gene001953:CDS
AAATCTCAACCTTAATTTGG+GGG 0.645557 8.3:+31510453 MS.gene001953:CDS
TTCCGGATACTACTTTCCCG+TGG 0.649132 8.3:+31511628 MS.gene001953:CDS
CCAAATAGATGGGTAGAATG+GGG 0.657480 8.3:+31512029 MS.gene001953:CDS
TCCTTACATTCACAACCACT+AGG 0.658950 8.3:-31511675 None:intergenic
CATCAGAGTGGGGAACCTAG+TGG 0.659288 8.3:+31511660 MS.gene001953:CDS
TTGCATATAACATGACATGA+GGG 0.662482 8.3:-31512226 None:intergenic
GGCGAGAGAAGAATTCCTCA+AGG 0.664502 8.3:+31511205 MS.gene001953:CDS
GAAACCGTGTTGCACAAAAG+GGG 0.665958 8.3:+31511832 MS.gene001953:CDS
TGATGTTCCGATGGATATGG+TGG 0.669643 8.3:-31510200 None:intergenic
ATGAGAAATACTAAGATCGG+GGG 0.677362 8.3:-31510122 None:intergenic
TAGTGGTTGTGAATGTAAGG+AGG 0.682180 8.3:+31511677 MS.gene001953:CDS
TAGGGTGTCGGCTACCAACG+TGG 0.694009 8.3:+31510843 MS.gene001953:CDS
AATTTGTGATCAGAAATTGG+AGG 0.699714 8.3:+31511230 MS.gene001953:CDS
TGTGGAGGCAGAGATGAGAG+TGG 0.704475 8.3:+31510810 MS.gene001953:CDS
AAATGATCGAGCTATTGTTG+TGG 0.743063 8.3:+31510495 MS.gene001953:CDS
GATGACATGGAATTAGACGA+TGG 0.783210 8.3:+31511153 MS.gene001953:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGATATTTCAAATAATA+GGG + chr8.3:31511539-31511558 MS.gene001953:CDS 10.0%
!! CTATTGATATTTCAAATAAT+AGG + chr8.3:31511538-31511557 MS.gene001953:CDS 15.0%
!! ACATACTGAATGAATATATT+TGG - chr8.3:31512179-31512198 None:intergenic 20.0%
!! TATAAGTTATTGAGAATTGA+GGG + chr8.3:31511429-31511448 MS.gene001953:CDS 20.0%
!!! CTCAAATTTTAACAATGAAT+GGG + chr8.3:31511979-31511998 MS.gene001953:CDS 20.0%
!!! TCAAATTTTAACAATGAATG+GGG + chr8.3:31511980-31511999 MS.gene001953:CDS 20.0%
! ACATCCAGAAAATAATCTAA+AGG - chr8.3:31512110-31512129 None:intergenic 25.0%
! AGAAATAGAAGTAGAAGAAT+GGG - chr8.3:31510068-31510087 None:intergenic 25.0%
! ATAAGTTATTGAGAATTGAG+GGG + chr8.3:31511430-31511449 MS.gene001953:CDS 25.0%
! ATATCATTAAATAGCCTAAC+TGG - chr8.3:31511573-31511592 None:intergenic 25.0%
! CTAAATCTCAACCTTAATTT+GGG + chr8.3:31510451-31510470 MS.gene001953:CDS 25.0%
! CTAGGGATATTAAGAAAATT+GGG + chr8.3:31511487-31511506 MS.gene001953:CDS 25.0%
! GTATAAGTTATTGAGAATTG+AGG + chr8.3:31511428-31511447 MS.gene001953:CDS 25.0%
! TAGTACATTTCCAAATAGAT+GGG + chr8.3:31512019-31512038 MS.gene001953:CDS 25.0%
! TCTTTATTATGAGTATCTTC+CGG + chr8.3:31511611-31511630 MS.gene001953:CDS 25.0%
! TGAAACAACAAAATCATTGT+TGG - chr8.3:31510398-31510417 None:intergenic 25.0%
! TTGATGAGAAATACTAAGAT+CGG - chr8.3:31510128-31510147 None:intergenic 25.0%
!! GATTTAAGGTTGAAATTCTA+GGG - chr8.3:31510153-31510172 None:intergenic 25.0%
!! TAAATCTCAACCTTAATTTG+GGG + chr8.3:31510452-31510471 MS.gene001953:CDS 25.0%
!! TCAAAGAGTATGTCTTATAA+TGG + chr8.3:31511087-31511106 MS.gene001953:CDS 25.0%
!! TGATTTAAGGTTGAAATTCT+AGG - chr8.3:31510154-31510173 None:intergenic 25.0%
!!! AAACCTTTGATTTTTGAATG+CGG + chr8.3:31511780-31511799 MS.gene001953:CDS 25.0%
!!! CAATGATTTTGTTGTTTCAT+TGG + chr8.3:31510399-31510418 MS.gene001953:CDS 25.0%
!!! GCTCAAATTTTAACAATGAA+TGG + chr8.3:31511978-31511997 MS.gene001953:CDS 25.0%
AATGAATGAGGAGAAGAAAT+TGG + chr8.3:31510699-31510718 MS.gene001953:CDS 30.0%
AATTTGTGATCAGAAATTGG+AGG + chr8.3:31511230-31511249 MS.gene001953:CDS 30.0%
ACAATTACACTAGTAGCAAT+CGG - chr8.3:31511117-31511136 None:intergenic 30.0%
AGAAATTGTTAAAAGGTTCC+AGG - chr8.3:31510249-31510268 None:intergenic 30.0%
ATATATTTGGACTCGAACTA+TGG - chr8.3:31512166-31512185 None:intergenic 30.0%
ATTGCATATAACATGACATG+AGG - chr8.3:31512230-31512249 None:intergenic 30.0%
ATTTGTGATCAGAAATTGGA+GGG + chr8.3:31511231-31511250 MS.gene001953:CDS 30.0%
CATCTATTTGGAAATGTACT+AGG - chr8.3:31512020-31512039 None:intergenic 30.0%
CCAAATTAAGGTTGAGATTT+AGG - chr8.3:31510453-31510472 None:intergenic 30.0%
CCTAAATCTCAACCTTAATT+TGG + chr8.3:31510450-31510469 MS.gene001953:CDS 30.0%
CTAGTACATTTCCAAATAGA+TGG + chr8.3:31512018-31512037 MS.gene001953:CDS 30.0%
GAGAAATAGAAGTAGAAGAA+TGG - chr8.3:31510069-31510088 None:intergenic 30.0%
GCAAATTTGTGATCAGAAAT+TGG + chr8.3:31511227-31511246 MS.gene001953:CDS 30.0%
GCTAGGGATATTAAGAAAAT+TGG + chr8.3:31511486-31511505 MS.gene001953:CDS 30.0%
GGAAGATACTCATAATAAAG+AGG - chr8.3:31511612-31511631 None:intergenic 30.0%
GGATATTAAGAAAATTGGGT+TGG + chr8.3:31511491-31511510 MS.gene001953:CDS 30.0%
GGTGATATTATAATCTACAC+TGG + chr8.3:31511174-31511193 MS.gene001953:CDS 30.0%
GTTAGTTGCTTGTTTGATAT+AGG + chr8.3:31511381-31511400 MS.gene001953:CDS 30.0%
TCAATAGAGATACAACTCAT+TGG - chr8.3:31511525-31511544 None:intergenic 30.0%
TCATGTCATGTTATATGCAA+TGG + chr8.3:31512229-31512248 MS.gene001953:CDS 30.0%
TCCAGAAAATAATCTAAAGG+AGG - chr8.3:31512107-31512126 None:intergenic 30.0%
TGATGAGAAATACTAAGATC+GGG - chr8.3:31510127-31510146 None:intergenic 30.0%
TTGCATATAACATGACATGA+GGG - chr8.3:31512229-31512248 None:intergenic 30.0%
TTGTTTGATATAGGAAAGTC+TGG + chr8.3:31511390-31511409 MS.gene001953:CDS 30.0%
! AAATCTCAACCTTAATTTGG+GGG + chr8.3:31510453-31510472 MS.gene001953:CDS 30.0%
! AATTGGTTGTTGAAGTTGAT+AGG + chr8.3:31510716-31510735 MS.gene001953:CDS 30.0%
! ACCTCCTTTAGATTATTTTC+TGG + chr8.3:31512103-31512122 MS.gene001953:CDS 30.0%
! AGAGAAATTAGGTTGTTGTT+GGG - chr8.3:31510287-31510306 None:intergenic 30.0%
! TTTGATGGCATATATGGTTA+TGG + chr8.3:31510540-31510559 MS.gene001953:CDS 30.0%
!! AATATAGCTTGGAAGTGTTT+AGG + chr8.3:31511859-31511878 MS.gene001953:CDS 30.0%
!! ATTGGTTGTTGAAGTTGATA+GGG + chr8.3:31510717-31510736 MS.gene001953:CDS 30.0%
!! TAATCTAAAGGAGGTATTGA+CGG - chr8.3:31512098-31512117 None:intergenic 30.0%
!! TGATGTGTTTTTGTTTAGGA+GGG + chr8.3:31511011-31511030 MS.gene001953:CDS 30.0%
!! TGTTGTTTCATTGGAGAATA+CGG + chr8.3:31510408-31510427 MS.gene001953:CDS 30.0%
!!! TTGGTGATGTGTTTTTGTTT+AGG + chr8.3:31511007-31511026 MS.gene001953:CDS 30.0%
AAATGATCGAGCTATTGTTG+TGG + chr8.3:31510495-31510514 MS.gene001953:CDS 35.0%
AATCAGCGATTTCGAGAAAA+GGG - chr8.3:31510375-31510394 None:intergenic 35.0%
ACATCACCAACATAAATTCC+AGG - chr8.3:31510997-31511016 None:intergenic 35.0%
ACCACATTCATGGTTATATG+AGG + chr8.3:31510905-31510924 MS.gene001953:CDS 35.0%
ACTAGTGTAATTGTTTCAGG+TGG + chr8.3:31511123-31511142 MS.gene001953:CDS 35.0%
AGAGAACTCTGCATAGATTA+TGG + chr8.3:31512269-31512288 MS.gene001953:CDS 35.0%
ATAGGGAAAATGTTCCAGTT+AGG + chr8.3:31511556-31511575 MS.gene001953:CDS 35.0%
ATGAGAAATACTAAGATCGG+GGG - chr8.3:31510125-31510144 None:intergenic 35.0%
ATTATAATCTACACTGGCCA+TGG + chr8.3:31511180-31511199 MS.gene001953:CDS 35.0%
ATTGGATGAGGAGAAGATAT+TGG + chr8.3:31510771-31510790 MS.gene001953:CDS 35.0%
ATTGTCTGAGGAAAGGAAAT+TGG + chr8.3:31510753-31510772 MS.gene001953:CDS 35.0%
CCAACATAAATTCCAGGTAT+CGG - chr8.3:31510991-31511010 None:intergenic 35.0%
CCACATTCATGGTTATATGA+GGG + chr8.3:31510906-31510925 MS.gene001953:CDS 35.0%
GACGCGAAGAAATTGTTAAA+AGG - chr8.3:31510256-31510275 None:intergenic 35.0%
GATGAGAAATACTAAGATCG+GGG - chr8.3:31510126-31510145 None:intergenic 35.0%
GCTACTAGTGTAATTGTTTC+AGG + chr8.3:31511120-31511139 MS.gene001953:CDS 35.0%
GGGAGACAATTAATGGAATA+AGG + chr8.3:31510869-31510888 MS.gene001953:CDS 35.0%
GGTAAGGTTTATGTTTACGA+TGG + chr8.3:31511345-31511364 MS.gene001953:CDS 35.0%
TAGAATTTGGCGTATAGGTT+GGG - chr8.3:31510334-31510353 None:intergenic 35.0%
TATCGTGATCAAAGGATAGT+TGG + chr8.3:31510964-31510983 MS.gene001953:CDS 35.0%
TATGGTTGTATCGTGATCAA+AGG + chr8.3:31510956-31510975 MS.gene001953:CDS 35.0%
TCCAAATAGATGGGTAGAAT+GGG + chr8.3:31512028-31512047 MS.gene001953:CDS 35.0%
TGCACTTTCGTCATAAGATA+AGG + chr8.3:31510623-31510642 MS.gene001953:CDS 35.0%
TTAAGGTTGAGATTTAGGTC+CGG - chr8.3:31510448-31510467 None:intergenic 35.0%
TTCCAAATAGATGGGTAGAA+TGG + chr8.3:31512027-31512046 MS.gene001953:CDS 35.0%
TTCGTCATAAGATAAGGAAG+AGG + chr8.3:31510629-31510648 MS.gene001953:CDS 35.0%
TTGTTAAAAGGTTCCAGGTT+TGG - chr8.3:31510244-31510263 None:intergenic 35.0%
! AAGCAATCCATGTGGATTAT+AGG - chr8.3:31511751-31511770 None:intergenic 35.0%
! AAGTAGAAGAATGGGTTTCA+AGG - chr8.3:31510060-31510079 None:intergenic 35.0%
! AATGGGTTTCAAGGGTTAAT+GGG - chr8.3:31510051-31510070 None:intergenic 35.0%
! AATTGTTTCAGGTGGTTATG+AGG + chr8.3:31511131-31511150 MS.gene001953:CDS 35.0%
! AGCAATCCATGTGGATTATA+GGG - chr8.3:31511750-31511769 None:intergenic 35.0%
! AGTAGAAGAATGGGTTTCAA+GGG - chr8.3:31510059-31510078 None:intergenic 35.0%
! GAGAGAAATTAGGTTGTTGT+TGG - chr8.3:31510288-31510307 None:intergenic 35.0%
!! ATACTGGTGTTGCTATAACT+AGG + chr8.3:31511949-31511968 MS.gene001953:CDS 35.0%
!! CACATGGATTGCTTTTGAAA+GGG + chr8.3:31511757-31511776 MS.gene001953:CDS 35.0%
!! GATATAGGAAAGTCTGGTTT+TGG + chr8.3:31511396-31511415 MS.gene001953:CDS 35.0%
!! GTGATGTGTTTTTGTTTAGG+AGG + chr8.3:31511010-31511029 MS.gene001953:CDS 35.0%
!! TACTGGTGTTGCTATAACTA+GGG + chr8.3:31511950-31511969 MS.gene001953:CDS 35.0%
!! TTGATCATCGGTTTCAGATT+CGG - chr8.3:31510311-31510330 None:intergenic 35.0%
!!! TTTTGAGGTGTGGTAGAATT+TGG - chr8.3:31510347-31510366 None:intergenic 35.0%
AAAGGGGCTCAAATATAGCT+TGG + chr8.3:31511848-31511867 MS.gene001953:CDS 40.0%
AAGTGATGTTCCGATGGATA+TGG - chr8.3:31510206-31510225 None:intergenic 40.0%
ACCAGTATACTCGCAGATAA+AGG - chr8.3:31511937-31511956 None:intergenic 40.0%
ACCTAGTGGTTGTGAATGTA+AGG + chr8.3:31511674-31511693 MS.gene001953:CDS 40.0%
ACTCGAACTATGGCTCATAT+AGG - chr8.3:31512156-31512175 None:intergenic 40.0%
AGAAGAGATTGTCTGAGGAA+AGG + chr8.3:31510746-31510765 MS.gene001953:CDS 40.0%
ATTGAGGTTAGGGTTATTCG+CGG + chr8.3:31511291-31511310 MS.gene001953:CDS 40.0%
CAAATCACCACCATATCCAT+CGG + chr8.3:31510193-31510212 MS.gene001953:CDS 40.0%
CACTGTTCTCAAAGATGCTA+GGG + chr8.3:31511470-31511489 MS.gene001953:CDS 40.0%
CCAAATAGATGGGTAGAATG+GGG + chr8.3:31512029-31512048 MS.gene001953:CDS 40.0%
CCCTCATATAACCATGAATG+TGG - chr8.3:31510909-31510928 None:intergenic 40.0%
CCGATACCTGGAATTTATGT+TGG + chr8.3:31510988-31511007 MS.gene001953:CDS 40.0%
CCTTTCAAAAGCAATCCATG+TGG - chr8.3:31511759-31511778 None:intergenic 40.0%
CGTAGTATCAACCACATTCA+TGG + chr8.3:31510895-31510914 MS.gene001953:CDS 40.0%
CTCTGATGAAACACAAACCA+CGG - chr8.3:31511648-31511667 None:intergenic 40.0%
CTGATCACAAATTTGCCTTG+AGG - chr8.3:31511223-31511242 None:intergenic 40.0%
GAAACAAGAGTCGAGTTTGA+TGG + chr8.3:31510525-31510544 MS.gene001953:CDS 40.0%
GAATCAGCGATTTCGAGAAA+AGG - chr8.3:31510376-31510395 None:intergenic 40.0%
GAGTTTCCCTATAATCCACA+TGG + chr8.3:31511741-31511760 MS.gene001953:CDS 40.0%
GATGACATGGAATTAGACGA+TGG + chr8.3:31511153-31511172 MS.gene001953:CDS 40.0%
GGAATAGGAACATGAGAAGA+AGG - chr8.3:31510094-31510113 None:intergenic 40.0%
GGCATATATGGTTATGGAGA+AGG + chr8.3:31510546-31510565 MS.gene001953:CDS 40.0%
GGTTTCAGATCTGAGTATGA+AGG + chr8.3:31510594-31510613 MS.gene001953:CDS 40.0%
GGTTTGTGTTTCATCAGAGT+GGG + chr8.3:31511649-31511668 MS.gene001953:CDS 40.0%
GTAGAATTTGGCGTATAGGT+TGG - chr8.3:31510335-31510354 None:intergenic 40.0%
GTTTCAAGGGTTAATGGGAA+AGG - chr8.3:31510046-31510065 None:intergenic 40.0%
GTTTGTGTTTCATCAGAGTG+GGG + chr8.3:31511650-31511669 MS.gene001953:CDS 40.0%
TAAAGGATCCAGCCTGAATA+AGG - chr8.3:31511920-31511939 None:intergenic 40.0%
TAAGGTTGAGATTTAGGTCC+GGG - chr8.3:31510447-31510466 None:intergenic 40.0%
TACGGTAAATGTTTGCAACC+CGG + chr8.3:31510426-31510445 MS.gene001953:CDS 40.0%
TAGGAGGACATGAGCAAAAA+GGG - chr8.3:31511806-31511825 None:intergenic 40.0%
TAGTGGTTGTGAATGTAAGG+AGG + chr8.3:31511677-31511696 MS.gene001953:CDS 40.0%
TCAGATTCGGGAGAGAAATT+AGG - chr8.3:31510298-31510317 None:intergenic 40.0%
TCCTTACATTCACAACCACT+AGG - chr8.3:31511678-31511697 None:intergenic 40.0%
TCCTTTATCTGCGAGTATAC+TGG + chr8.3:31511933-31511952 MS.gene001953:CDS 40.0%
TCTATGCAGAGTTCTCTCAT+AGG - chr8.3:31512266-31512285 None:intergenic 40.0%
TCTGATGAAACACAAACCAC+GGG - chr8.3:31511647-31511666 None:intergenic 40.0%
TGAGGAAAGGAAATTGGATG+AGG + chr8.3:31510759-31510778 MS.gene001953:CDS 40.0%
TGTGGTAGAATTTGGCGTAT+AGG - chr8.3:31510339-31510358 None:intergenic 40.0%
TTAGGAGGACATGAGCAAAA+AGG - chr8.3:31511807-31511826 None:intergenic 40.0%
TTGCATATGCATATGCAAGC+TGG + chr8.3:31511051-31511070 MS.gene001953:CDS 40.0%
! AAACTCGACTCTTGTTTCTC+AGG - chr8.3:31510523-31510542 None:intergenic 40.0%
! AGGTTGTTGTTGGGAATCTT+GGG - chr8.3:31510278-31510297 None:intergenic 40.0%
! ATGGATATGGTGGTGATTTG+GGG - chr8.3:31510193-31510212 None:intergenic 40.0%
! GAATGGGTTTCAAGGGTTAA+TGG - chr8.3:31510052-31510071 None:intergenic 40.0%
! GAGAAAAGGGTAGAGTTTTG+AGG - chr8.3:31510362-31510381 None:intergenic 40.0%
! GATGGATATGGTGGTGATTT+GGG - chr8.3:31510194-31510213 None:intergenic 40.0%
! GTCGAGTTTGATGGCATATA+TGG + chr8.3:31510534-31510553 MS.gene001953:CDS 40.0%
! TAGGTTGTTGTTGGGAATCT+TGG - chr8.3:31510279-31510298 None:intergenic 40.0%
! TGCTTTTGCGCTATGAAGAA+TGG + chr8.3:31511714-31511733 MS.gene001953:CDS 40.0%
! TGGAAAGGAGTATGCACTAT+GGG + chr8.3:31511268-31511287 MS.gene001953:CDS 40.0%
! TGGTTTGTGTTTCATCAGAG+TGG + chr8.3:31511648-31511667 MS.gene001953:CDS 40.0%
! TTGGAAAGGAGTATGCACTA+TGG + chr8.3:31511267-31511286 MS.gene001953:CDS 40.0%
! TTTTGCGCTATGAAGAATGG+AGG + chr8.3:31511717-31511736 MS.gene001953:CDS 40.0%
!! CCACATGGATTGCTTTTGAA+AGG + chr8.3:31511756-31511775 MS.gene001953:CDS 40.0%
!! GTATAGGTTGGGTTGATCAT+CGG - chr8.3:31510323-31510342 None:intergenic 40.0%
!! TGATCATCGGTTTCAGATTC+GGG - chr8.3:31510310-31510329 None:intergenic 40.0%
AACAATGAATGGGGTCTCGT+TGG + chr8.3:31511989-31512008 MS.gene001953:CDS 45.0%
AACCACGGGAAAGTAGTATC+CGG - chr8.3:31511633-31511652 None:intergenic 45.0%
ACACGGTTTCGACAATTAGG+AGG - chr8.3:31511822-31511841 None:intergenic 45.0%
AGGTGGTTATGAGGATGACA+TGG + chr8.3:31511140-31511159 MS.gene001953:CDS 45.0%
ATAATCTACACTGGCCATGG+AGG + chr8.3:31511183-31511202 MS.gene001953:CDS 45.0%
ATGCAGAGTTCTCTCATAGG+AGG - chr8.3:31512263-31512282 None:intergenic 45.0%
ATTCGCGGGATGAAGTATGA+AGG + chr8.3:31511306-31511325 MS.gene001953:CDS 45.0%
CAGAATCCACCCCCAAATTA+AGG - chr8.3:31510465-31510484 None:intergenic 45.0%
CCCCATTCTACCCATCTATT+TGG - chr8.3:31512032-31512051 None:intergenic 45.0%
CGAAACCGTGTTGCACAAAA+GGG + chr8.3:31511831-31511850 MS.gene001953:CDS 45.0%
CTGCATAGATTATGGCATCC+CGG + chr8.3:31512277-31512296 MS.gene001953:CDS 45.0%
GAAACCGTGTTGCACAAAAG+GGG + chr8.3:31511832-31511851 MS.gene001953:CDS 45.0%
GAGTATGCACTATGGGATTG+AGG + chr8.3:31511275-31511294 MS.gene001953:CDS 45.0%
GAGTGCATCTACTTCTGGTA+AGG + chr8.3:31511329-31511348 MS.gene001953:CDS 45.0%
GCAACACGGTTTCGACAATT+AGG - chr8.3:31511825-31511844 None:intergenic 45.0%
GCACTATGGGATTGAGGTTA+GGG + chr8.3:31511281-31511300 MS.gene001953:CDS 45.0%
GCACTGTTCTCAAAGATGCT+AGG + chr8.3:31511469-31511488 MS.gene001953:CDS 45.0%
GCATACTCCTTTCCAATGCA+AGG - chr8.3:31511263-31511282 None:intergenic 45.0%
GGAAGAGAAGAGATTGTCTG+AGG + chr8.3:31510741-31510760 MS.gene001953:CDS 45.0%
GGAGGACAAGAGAATGAATG+AGG + chr8.3:31510687-31510706 MS.gene001953:CDS 45.0%
GGGCCGCATTCAAAAATCAA+AGG - chr8.3:31511786-31511805 None:intergenic 45.0%
GTGGAAACCTTGCATTGGAA+AGG + chr8.3:31511253-31511272 MS.gene001953:CDS 45.0%
GTGTTTAGGTCTACACAGAC+AGG + chr8.3:31511873-31511892 MS.gene001953:CDS 45.0%
GTTATTGAGAATTGAGGGGC+AGG + chr8.3:31511434-31511453 MS.gene001953:CDS 45.0%
TAATCTACACTGGCCATGGA+GGG + chr8.3:31511184-31511203 MS.gene001953:CDS 45.0%
TAGGTCCTTGGACCTTATTC+AGG + chr8.3:31511905-31511924 MS.gene001953:CDS 45.0%
TAGGTCTACACAGACAGGTT+GGG + chr8.3:31511878-31511897 MS.gene001953:CDS 45.0%
TCGAAACCGTGTTGCACAAA+AGG + chr8.3:31511830-31511849 MS.gene001953:CDS 45.0%
TGATGTTCCGATGGATATGG+TGG - chr8.3:31510203-31510222 None:intergenic 45.0%
TGCACTATGGGATTGAGGTT+AGG + chr8.3:31511280-31511299 MS.gene001953:CDS 45.0%
TGCAGAGTTCTCTCATAGGA+GGG - chr8.3:31512262-31512281 None:intergenic 45.0%
TGTAAGGAGGCTTGTACTGA+CGG + chr8.3:31511690-31511709 MS.gene001953:CDS 45.0%
TGTGATCAGAAATTGGAGGG+TGG + chr8.3:31511234-31511253 MS.gene001953:CDS 45.0%
TTAGGTCTACACAGACAGGT+TGG + chr8.3:31511877-31511896 MS.gene001953:CDS 45.0%
TTCGCGGGATGAAGTATGAA+GGG + chr8.3:31511307-31511326 MS.gene001953:CDS 45.0%
TTGAGGTTAGGGTTATTCGC+GGG + chr8.3:31511292-31511311 MS.gene001953:CDS 45.0%
! ATATGGTGGTGATTTGGGGT+GGG - chr8.3:31510189-31510208 None:intergenic 45.0%
! CGACATTAATGAAGGAGCGT+GGG + chr8.3:31510932-31510951 MS.gene001953:CDS 45.0%
! CGATGGATATGGTGGTGATT+TGG - chr8.3:31510195-31510214 None:intergenic 45.0%
! CGTTTGAAGTGATGTTCCGA+TGG - chr8.3:31510212-31510231 None:intergenic 45.0%
! GGGGTGGATTCTGTAAATGA+TGG + chr8.3:31510472-31510491 MS.gene001953:CDS 45.0%
! TCTCAACCTTAATTTGGGGG+TGG + chr8.3:31510456-31510475 MS.gene001953:CDS 45.0%
!! GGAGGTATTGACGGATAAGA+TGG - chr8.3:31512089-31512108 None:intergenic 45.0%
!! GGTTGTTGAAGTTGATAGGG+AGG + chr8.3:31510720-31510739 MS.gene001953:CDS 45.0%
!!! AAGGGTAGAGTTTTGAGGTG+TGG - chr8.3:31510357-31510376 None:intergenic 45.0%
AACGCCACTCATTCCAAACC+TGG + chr8.3:31510228-31510247 MS.gene001953:CDS 50.0%
AATTCTTCTCTCGCCCTCCA+TGG - chr8.3:31511200-31511219 None:intergenic 50.0%
AGGAGGGAATTGTGTGTGAC+TGG + chr8.3:31511027-31511046 MS.gene001953:CDS 50.0%
AGGATAGTTGGACCGATACC+TGG + chr8.3:31510976-31510995 MS.gene001953:CDS 50.0%
AGGTCTACACAGACAGGTTG+GGG + chr8.3:31511879-31511898 MS.gene001953:CDS 50.0%
CTAAGATCGGGGGTGATAGT+AGG - chr8.3:31510115-31510134 None:intergenic 50.0%
GAGAGTGGGTGAGGAGAATA+GGG + chr8.3:31510825-31510844 MS.gene001953:CDS 50.0%
GAGGGCTGCGACATTAATGA+AGG + chr8.3:31510924-31510943 MS.gene001953:CDS 50.0%
GCGACATTAATGAAGGAGCG+TGG + chr8.3:31510931-31510950 MS.gene001953:CDS 50.0%
GGATGCAGAAGTTGCAGTTG+TGG + chr8.3:31510792-31510811 MS.gene001953:CDS 50.0%
GGCGAGAGAAGAATTCCTCA+AGG + chr8.3:31511205-31511224 MS.gene001953:CDS 50.0%
GGTGAGAGCGGTGTGATTTA+AGG - chr8.3:31510167-31510186 None:intergenic 50.0%
GGTTATGGAGAAGGAAAGCC+AGG + chr8.3:31510555-31510574 MS.gene001953:CDS 50.0%
GTGGAGGGGGAGACAATTAA+TGG + chr8.3:31510862-31510881 MS.gene001953:CDS 50.0%
TCCAGCCTGAATAAGGTCCA+AGG - chr8.3:31511913-31511932 None:intergenic 50.0%
TCCTTGGACCTTATTCAGGC+TGG + chr8.3:31511909-31511928 MS.gene001953:CDS 50.0%
TCGGGGGTGATAGTAGGAAT+AGG - chr8.3:31510109-31510128 None:intergenic 50.0%
TGAGAGTGGGTGAGGAGAAT+AGG + chr8.3:31510824-31510843 MS.gene001953:CDS 50.0%
TGCAGAAGTTGCAGTTGTGG+AGG + chr8.3:31510795-31510814 MS.gene001953:CDS 50.0%
TTCCGGATACTACTTTCCCG+TGG + chr8.3:31511628-31511647 MS.gene001953:CDS 50.0%
TTCGCTTCGCGTGAGAATTG+AGG + chr8.3:31510666-31510685 MS.gene001953:CDS 50.0%
! GAAGGGAGTGCATCTACTTC+TGG + chr8.3:31511324-31511343 MS.gene001953:CDS 50.0%
! GATATGGTGGTGATTTGGGG+TGG - chr8.3:31510190-31510209 None:intergenic 50.0%
! GGTTCCAGGTTTGGAATGAG+TGG - chr8.3:31510235-31510254 None:intergenic 50.0%
! TAATGAAGGAGCGTGGGCTA+TGG + chr8.3:31510938-31510957 MS.gene001953:CDS 50.0%
! TATGGTGGTGATTTGGGGTG+GGG - chr8.3:31510188-31510207 None:intergenic 50.0%
! TGAGCCCCTTTTGTGCAACA+CGG - chr8.3:31511839-31511858 None:intergenic 50.0%
!! CCAGGTGATGGAACTTGTGA+GGG + chr8.3:31510573-31510592 MS.gene001953:CDS 50.0%
AATTGAGGGGCAGGCGAAGA+TGG + chr8.3:31511443-31511462 MS.gene001953:CDS 55.0%
AATTGTCTCCCCCTCCACGT+TGG - chr8.3:31510860-31510879 None:intergenic 55.0%
AGGTTGGGGAGTTAGGTCCT+TGG + chr8.3:31511893-31511912 MS.gene001953:CDS 55.0%
ATTATGGCATCCCGGACGAG+TGG + chr8.3:31512285-31512304 MS.gene001953:CDS 55.0%
ATTGAGGGGCAGGCGAAGAT+GGG + chr8.3:31511444-31511463 MS.gene001953:CDS 55.0%
CATCAGAGTGGGGAACCTAG+TGG + chr8.3:31511660-31511679 MS.gene001953:CDS 55.0%
CCCTCACAAGTTCCATCACC+TGG - chr8.3:31510576-31510595 None:intergenic 55.0%
GCTTCGCGTGAGAATTGAGG+AGG + chr8.3:31510669-31510688 MS.gene001953:CDS 55.0%
GGAGAAGGAAAGCCAGGTGA+TGG + chr8.3:31510561-31510580 MS.gene001953:CDS 55.0%
GGAGGGTGGAAACCTTGCAT+TGG + chr8.3:31511248-31511267 MS.gene001953:CDS 55.0%
GGGTGAGGAGAATAGGGTGT+CGG + chr8.3:31510831-31510850 MS.gene001953:CDS 55.0%
GTGGAGGCAGAGATGAGAGT+GGG + chr8.3:31510811-31510830 MS.gene001953:CDS 55.0%
TGTGGAGGCAGAGATGAGAG+TGG + chr8.3:31510810-31510829 MS.gene001953:CDS 55.0%
TTATGGCATCCCGGACGAGT+GGG + chr8.3:31512286-31512305 MS.gene001953:CDS 55.0%
! CACAGACAGGTTGGGGAGTT+AGG + chr8.3:31511886-31511905 MS.gene001953:CDS 55.0%
!! GCCAGGTGATGGAACTTGTG+AGG + chr8.3:31510572-31510591 MS.gene001953:CDS 55.0%
ATCTTGCTCGCCCACTCGTC+CGG - chr8.3:31512299-31512318 None:intergenic 60.0%
GGCAGAGATGAGAGTGGGTG+AGG + chr8.3:31510816-31510835 MS.gene001953:CDS 60.0%
! GATTTGGGGTGGGGTGAGAG+CGG - chr8.3:31510179-31510198 None:intergenic 60.0%
! TGTCGGCTACCAACGTGGAG+GGG + chr8.3:31510848-31510867 MS.gene001953:CDS 60.0%
!! GTGTCGGCTACCAACGTGGA+GGG + chr8.3:31510847-31510866 MS.gene001953:CDS 60.0%
!! TAGGGTGTCGGCTACCAACG+TGG + chr8.3:31510843-31510862 MS.gene001953:CDS 60.0%
GTCGGCTACCAACGTGGAGG+GGG + chr8.3:31510849-31510868 MS.gene001953:CDS 65.0%
TCTTGCTCGCCCACTCGTCC+GGG - chr8.3:31512298-31512317 None:intergenic 65.0%
!! GGTGTCGGCTACCAACGTGG+AGG + chr8.3:31510846-31510865 MS.gene001953:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 31510037 31512334 31510037 ID=MS.gene001953
chr8.3 mRNA 31510037 31512334 31510037 ID=MS.gene001953.t1;Parent=MS.gene001953
chr8.3 exon 31510037 31512334 31510037 ID=MS.gene001953.t1.exon1;Parent=MS.gene001953.t1
chr8.3 CDS 31510037 31512334 31510037 ID=cds.MS.gene001953.t1;Parent=MS.gene001953.t1
Gene Sequence

>MS.gene001953

ATGGATCCTTTCCCATTAACCCTTGAAACCCATTCTTCTACTTCTATTTCTCAACCTTCTTCTCATGTTCCTATTCCTACTATCACCCCCGATCTTAGTATTTCTCATCAAATCCCTAGAATTTCAACCTTAAATCACACCGCTCTCACCCCACCCCAAATCACCACCATATCCATCGGAACATCACTTCAAACGCCACTCATTCCAAACCTGGAACCTTTTAACAATTTCTTCGCGTCCCAAGATTCCCAACAACAACCTAATTTCTCTCCCGAATCTGAAACCGATGATCAACCCAACCTATACGCCAAATTCTACCACACCTCAAAACTCTACCCTTTTCTCGAAATCGCTGATTCCAACAATGATTTTGTTGTTTCATTGGAGAATACGGTAAATGTTTGCAACCCGGACCTAAATCTCAACCTTAATTTGGGGGTGGATTCTGTAAATGATGGAAATGATCGAGCTATTGTTGTGGCTCCTGAGAAACAAGAGTCGAGTTTGATGGCATATATGGTTATGGAGAAGGAAAGCCAGGTGATGGAACTTGTGAGGGTTTCAGATCTGAGTATGAAGGATCAGATGCACTTTCGTCATAAGATAAGGAAGAGGCGAATGATTTATGATTCGCTTCGCGTGAGAATTGAGGAGGACAAGAGAATGAATGAGGAGAAGAAATTGGTTGTTGAAGTTGATAGGGAGGAAGAGAAGAGATTGTCTGAGGAAAGGAAATTGGATGAGGAGAAGATATTGGATGCAGAAGTTGCAGTTGTGGAGGCAGAGATGAGAGTGGGTGAGGAGAATAGGGTGTCGGCTACCAACGTGGAGGGGGAGACAATTAATGGAATAAGGCGTCGTAGTATCAACCACATTCATGGTTATATGAGGGCTGCGACATTAATGAAGGAGCGTGGGCTATGGTTGTATCGTGATCAAAGGATAGTTGGACCGATACCTGGAATTTATGTTGGTGATGTGTTTTTGTTTAGGAGGGAATTGTGTGTGACTGGTTTGCATATGCATATGCAAGCTGGTATTGATTATCTATCAAAGAGTATGTCTTATAATGGTGAACCGATTGCTACTAGTGTAATTGTTTCAGGTGGTTATGAGGATGACATGGAATTAGACGATGGTGATATTATAATCTACACTGGCCATGGAGGGCGAGAGAAGAATTCCTCAAGGCAAATTTGTGATCAGAAATTGGAGGGTGGAAACCTTGCATTGGAAAGGAGTATGCACTATGGGATTGAGGTTAGGGTTATTCGCGGGATGAAGTATGAAGGGAGTGCATCTACTTCTGGTAAGGTTTATGTTTACGATGGATTGTATAAGATTGTTAGTTGCTTGTTTGATATAGGAAAGTCTGGTTTTGGAGTTTATAAGTATAAGTTATTGAGAATTGAGGGGCAGGCGAAGATGGGTAGCACTGTTCTCAAAGATGCTAGGGATATTAAGAAAATTGGGTTGGATTTCATGCCAATGAGTTGTATCTCTATTGATATTTCAAATAATAGGGAAAATGTTCCAGTTAGGCTATTTAATGATATAGATGTCAATCATGAACCTCTTTATTATGAGTATCTTCCGGATACTACTTTCCCGTGGTTTGTGTTTCATCAGAGTGGGGAACCTAGTGGTTGTGAATGTAAGGAGGCTTGTACTGACGGTTGCTTTTGCGCTATGAAGAATGGAGGAGAGTTTCCCTATAATCCACATGGATTGCTTTTGAAAGGGAAACCTTTGATTTTTGAATGCGGCCCTTTTTGCTCATGTCCTCCTAATTGTCGAAACCGTGTTGCACAAAAGGGGCTCAAATATAGCTTGGAAGTGTTTAGGTCTACACAGACAGGTTGGGGAGTTAGGTCCTTGGACCTTATTCAGGCTGGATCCTTTATCTGCGAGTATACTGGTGTTGCTATAACTAGGGAACAAGCTCAAATTTTAACAATGAATGGGGTCTCGTTGGTGTATCCTAGTACATTTCCAAATAGATGGGTAGAATGGGGTGATTTATCTCAGATATACACTGAATATGTGCGACCATCTTATCCGTCAATACCTCCTTTAGATTATTTTCTGGATGTGTCAACAATGAGAAATGTTGCATCCTATATGAGCCATAGTTCGAGTCCAAATATATTCATTCAGTATGTTCTTTATGATCACAACAATTTGTTGTTCCCTCATGTCATGTTATATGCAATGGAGAACATCCCTCCTATGAGAGAACTCTGCATAGATTATGGCATCCCGGACGAGTGGGCGAGCAAGATGTCTATATGTATGTAA
Protein sequence

>MS.gene001953.t1

MDPFPLTLETHSSTSISQPSSHVPIPTITPDLSISHQIPRISTLNHTALTPPQITTISIGTSLQTPLIPNLEPFNNFFASQDSQQQPNFSPESETDDQPNLYAKFYHTSKLYPFLEIADSNNDFVVSLENTVNVCNPDLNLNLNLGVDSVNDGNDRAIVVAPEKQESSLMAYMVMEKESQVMELVRVSDLSMKDQMHFRHKIRKRRMIYDSLRVRIEEDKRMNEEKKLVVEVDREEEKRLSEERKLDEEKILDAEVAVVEAEMRVGEENRVSATNVEGETINGIRRRSINHIHGYMRAATLMKERGLWLYRDQRIVGPIPGIYVGDVFLFRRELCVTGLHMHMQAGIDYLSKSMSYNGEPIATSVIVSGGYEDDMELDDGDIIIYTGHGGREKNSSRQICDQKLEGGNLALERSMHYGIEVRVIRGMKYEGSASTSGKVYVYDGLYKIVSCLFDIGKSGFGVYKYKLLRIEGQAKMGSTVLKDARDIKKIGLDFMPMSCISIDISNNRENVPVRLFNDIDVNHEPLYYEYLPDTTFPWFVFHQSGEPSGCECKEACTDGCFCAMKNGGEFPYNPHGLLLKGKPLIFECGPFCSCPPNCRNRVAQKGLKYSLEVFRSTQTGWGVRSLDLIQAGSFICEYTGVAITREQAQILTMNGVSLVYPSTFPNRWVEWGDLSQIYTEYVRPSYPSIPPLDYFLDVSTMRNVASYMSHSSSPNIFIQYVLYDHNNLLFPHVMLYAMENIPPMRELCIDYGIPDEWASKMSICM