AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002033

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002033.t1 MTR_5g015480 69.626 214 43 3 1 214 1 192 1.25e-96 285
MS.gene002033.t1 MTR_6g017200 71.171 111 28 1 36 142 34 144 1.71e-47 156
MS.gene002033.t1 MTR_1g057920 63.636 121 40 1 27 143 1 121 2.56e-45 149
MS.gene002033.t1 MTR_2g070590 68.932 103 32 0 40 142 1 103 2.19e-43 144
MS.gene002033.t1 MTR_0402s0040 69.369 111 30 1 36 142 2 112 5.17e-43 154
MS.gene002033.t1 MTR_0137s0040 59.615 104 38 1 25 124 1 104 5.33e-36 125
MS.gene002033.t1 MTR_7g113010 49.600 125 45 2 26 146 423 533 3.07e-26 108
MS.gene002033.t1 MTR_0291s0060 65.217 69 20 1 38 102 13 81 7.66e-24 93.2
MS.gene002033.t1 MTR_4g102720 41.739 115 61 3 56 170 7 115 5.60e-20 86.7
MS.gene002033.t1 MTR_1g091013 67.857 56 18 0 69 124 14 69 1.30e-18 78.6
MS.gene002033.t1 MTR_4g067290 45.055 91 46 2 56 146 56 142 1.84e-16 77.4
MS.gene002033.t1 MTR_4g067290 45.055 91 46 2 56 146 56 142 1.86e-16 77.4
MS.gene002033.t1 MTR_4g067290 45.055 91 46 2 56 146 76 162 2.55e-16 77.4
MS.gene002033.t1 MTR_4g067290 45.055 91 46 2 56 146 74 160 2.60e-16 77.0
MS.gene002033.t1 MTR_4g067290 45.055 91 46 2 56 146 76 162 2.73e-16 77.0
MS.gene002033.t1 MTR_4g067290 45.055 91 46 2 56 146 116 202 3.67e-16 77.4
MS.gene002033.t1 MTR_2g098920 43.210 81 45 1 56 136 80 159 4.17e-15 73.9
MS.gene002033.t1 MTR_7g074790 44.578 83 44 2 56 137 10 91 1.45e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002033.t1 AT3G59470 46.914 81 42 1 56 136 70 149 9.41e-16 75.5
MS.gene002033.t1 AT3G59470 46.914 81 42 1 56 136 70 149 9.41e-16 75.5
MS.gene002033.t1 AT3G59470 46.914 81 42 1 56 136 82 161 1.14e-15 75.5

Find 65 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCAACTTGGCTTGAGAAAT+TGG 0.175914 2.3:+75934058 MS.gene002033:CDS
TGAACGAGAAAGGGCAGATT+TGG 0.279196 2.3:+75935858 MS.gene002033:CDS
CGCGACGGTGGCAGTGGCGG+AGG 0.371954 2.3:+75935936 MS.gene002033:CDS
TATAGTAGGAAATGTGGTTT+TGG 0.376590 2.3:+75933756 MS.gene002033:CDS
GGTGAATGATATCGTGCAAC+AGG 0.381657 2.3:+75933650 MS.gene002033:CDS
TCACCATTCATCACCACCTC+TGG 0.385515 2.3:+75933430 MS.gene002033:CDS
ATTTCACTTGATCGGAAGAT+TGG 0.399360 2.3:+75933939 MS.gene002033:CDS
GACACCGCGTCTACCCTCTT+TGG 0.402002 2.3:-75933847 None:intergenic
TAATGTACCACACTTGGAAA+TGG 0.424296 2.3:+75933692 MS.gene002033:CDS
TTAGAAAACATGAACGAGAA+AGG 0.427796 2.3:+75935848 MS.gene002033:CDS
TACACCTTCAACCATTGGAT+TGG 0.436578 2.3:-75933619 None:intergenic
ACGTGTGTCGTTTCTACCAT+TGG 0.448381 2.3:-75933389 None:intergenic
TCCAATTTCTCAAGCCAAGT+TGG 0.453093 2.3:-75934059 None:intergenic
TATGGTGTTGGCTTCTAGAA+AGG 0.456254 2.3:+75935896 MS.gene002033:CDS
GAACGAGAAAGGGCAGATTT+GGG 0.457260 2.3:+75935859 MS.gene002033:CDS
TGTGTACCATCATTGTTTGC+AGG 0.460822 2.3:-75934234 None:intergenic
GGACTTTGAGTCTGAGGCCT+TGG 0.461262 2.3:+75933713 MS.gene002033:CDS
AGAGCCGAGATTGCTTAGAT+AGG 0.461774 2.3:+75934021 MS.gene002033:CDS
AACAGGATGTTTAGCACGTA+TGG 0.468473 2.3:+75933914 MS.gene002033:CDS
TAGAAAACATGAACGAGAAA+GGG 0.477194 2.3:+75935849 MS.gene002033:CDS
AAGAGGCGCGACGGTGGCAG+TGG 0.486772 2.3:+75935930 MS.gene002033:CDS
GTGGACACTGTTGATCTTGG+TGG 0.493561 2.3:+75933984 MS.gene002033:CDS
AGGCGCGACGGTGGCAGTGG+CGG 0.494164 2.3:+75935933 MS.gene002033:CDS
TTTGCTTTGAGATGCGGCGA+CGG 0.494801 2.3:+75935961 MS.gene002033:CDS
GCGACGGTGGCAGTGGCGGA+GGG 0.509515 2.3:+75935937 MS.gene002033:CDS
GATGGTACACATGCTTTGAA+TGG 0.516924 2.3:+75934246 MS.gene002033:CDS
AAAAGACAGCAAAGACATGA+AGG 0.517398 2.3:-75935827 None:intergenic
GAGGGCTTTGCTTTGAGATG+CGG 0.525523 2.3:+75935955 MS.gene002033:CDS
ATGGTGTTGGCTTCTAGAAA+GGG 0.528676 2.3:+75935897 MS.gene002033:CDS
GCTTTGAGATGCGGCGACGG+CGG 0.528736 2.3:+75935964 MS.gene002033:CDS
CACCATTCATCACCACCTCT+GGG 0.529997 2.3:+75933431 MS.gene002033:CDS
TAGTCCTATCTAAGCAATCT+CGG 0.530700 2.3:-75934025 None:intergenic
ACATCCAATCCAATGGTTGA+AGG 0.531051 2.3:+75933615 MS.gene002033:CDS
GTATGGTTATTTCACTTGAT+CGG 0.532614 2.3:+75933931 MS.gene002033:CDS
TGCGGCGACGGCGGAAGTGA+CGG 0.535449 2.3:+75935973 MS.gene002033:CDS
GCTCGAGCAGAAACTAGAAC+AGG 0.536024 2.3:+75933897 MS.gene002033:CDS
AATGTGGACACTGTTGATCT+TGG 0.536764 2.3:+75933981 MS.gene002033:CDS
CAATCTCCTGCAAACAATGA+TGG 0.539918 2.3:+75934228 MS.gene002033:CDS
ACGTGATTACTTAACAAAAG+AGG 0.543276 2.3:+75933875 MS.gene002033:CDS
TGCCCAGAGGTGGTGATGAA+TGG 0.545146 2.3:-75933433 None:intergenic
GTACACATGCTTTGAATGGA+AGG 0.545764 2.3:+75934250 MS.gene002033:CDS
TGGACACTGTTGATCTTGGT+GGG 0.560318 2.3:+75933985 MS.gene002033:CDS
TGTTAGTAATGTACCACACT+TGG 0.560348 2.3:+75933686 MS.gene002033:CDS
ATTGAAGAATTAGAATGCCA+AGG 0.561345 2.3:+75935799 MS.gene002033:CDS
ATCTTACCGGTGCCCAGAGG+TGG 0.565589 2.3:-75933443 None:intergenic
GGAAATGGACTTTGAGTCTG+AGG 0.567953 2.3:+75933707 MS.gene002033:CDS
AACGAGTATAGTAGGAAATG+TGG 0.568624 2.3:+75933750 MS.gene002033:CDS
TGGGTCGTCACAGTAACATA+TGG 0.569464 2.3:+75935878 MS.gene002033:CDS
GTTATAAAATTCATATGCCA+AGG 0.577407 2.3:-75933730 None:intergenic
TTGAAGAATTAGAATGCCAA+GGG 0.582874 2.3:+75935800 MS.gene002033:CDS
AAAATTTGTGTGCTCCAAAG+AGG 0.589431 2.3:+75933833 MS.gene002033:CDS
TCATCACCACCTCTGGGCAC+CGG 0.597509 2.3:+75933437 MS.gene002033:CDS
TGTTGGCTTCTAGAAAGGGT+AGG 0.602571 2.3:+75935901 MS.gene002033:CDS
GTCACAGTAACATATGGTGT+TGG 0.612986 2.3:+75935884 MS.gene002033:CDS
GAATATTACATCCAATCCAA+TGG 0.614280 2.3:+75933608 MS.gene002033:CDS
AAATTTGTGTGCTCCAAAGA+GGG 0.614397 2.3:+75933834 MS.gene002033:CDS
AGGTTTGCAAAGAGGCGCGA+CGG 0.630884 2.3:+75935921 MS.gene002033:CDS
TCAAAGTCCATTTCCAAGTG+TGG 0.632466 2.3:-75933699 None:intergenic
GAAAGGGTAGGTTTGCAAAG+AGG 0.637487 2.3:+75935913 MS.gene002033:CDS
TTTGCAAAGAGGCGCGACGG+TGG 0.639189 2.3:+75935924 MS.gene002033:CDS
TGCTCCAAAGAGGGTAGACG+CGG 0.660484 2.3:+75933843 MS.gene002033:CDS
GTGAATGATATCGTGCAACA+GGG 0.667513 2.3:+75933651 MS.gene002033:CDS
GGCGACGGCGGAAGTGACGG+AGG 0.681192 2.3:+75935976 MS.gene002033:CDS
GGACACTGTTGATCTTGGTG+GGG 0.681245 2.3:+75933986 MS.gene002033:CDS
AAAATCTTACCGGTGCCCAG+AGG 0.742952 2.3:-75933446 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATGATAATATCATAAT+TGG - chr2.3:75935631-75935650 None:intergenic 10.0%
!! TATATGATAATATCATAATT+GGG - chr2.3:75935630-75935649 None:intergenic 10.0%
!! TGTTAAAAAAAATATGAATA+TGG + chr2.3:75934921-75934940 MS.gene002033:intron 10.0%
!!! CTACTTTTATCTTATAATTT+GGG + chr2.3:75934746-75934765 MS.gene002033:intron 15.0%
!! AATACAATTCAATACAATGT+TGG + chr2.3:75935471-75935490 MS.gene002033:intron 20.0%
!! AATTGTTCATATCAATTTAG+AGG - chr2.3:75935751-75935770 None:intergenic 20.0%
!! ATACAATTCAATACAATGTT+GGG + chr2.3:75935472-75935491 MS.gene002033:intron 20.0%
!! TATTCACATTATCTAGAAAA+TGG - chr2.3:75933593-75933612 None:intergenic 20.0%
!! TATTTCACATTATAGCAAAA+TGG - chr2.3:75934816-75934835 None:intergenic 20.0%
!!! AATTTTATAACGAGTATAGT+AGG + chr2.3:75933742-75933761 MS.gene002033:CDS 20.0%
!!! ACATTACTACTTTTTCTATT+AGG + chr2.3:75934653-75934672 MS.gene002033:intron 20.0%
!!! ATTACTACTTTTTCTATTAG+GGG + chr2.3:75934655-75934674 MS.gene002033:intron 20.0%
!!! CATGAATATTTGAAAGTATT+TGG + chr2.3:75933500-75933519 MS.gene002033:intron 20.0%
!!! CATTACTACTTTTTCTATTA+GGG + chr2.3:75934654-75934673 MS.gene002033:intron 20.0%
!!! GCTACTTTTATCTTATAATT+TGG + chr2.3:75934745-75934764 MS.gene002033:intron 20.0%
!!! TAATTTTATTGACAACGAAT+GGG + chr2.3:75935008-75935027 MS.gene002033:intron 20.0%
!!! TCATATGTGTTTTAGATTTT+GGG - chr2.3:75935450-75935469 None:intergenic 20.0%
!!! TCTGATATACTAGTTTATTT+TGG + chr2.3:75934723-75934742 MS.gene002033:intron 20.0%
! AAACAAACCAATACAAACAA+GGG + chr2.3:75935068-75935087 MS.gene002033:intron 25.0%
! AAGGAAGAGAAAGAAAAAAT+TGG + chr2.3:75934269-75934288 MS.gene002033:CDS 25.0%
! AGCATATCAAATGTAATGTT+TGG - chr2.3:75934989-75935008 None:intergenic 25.0%
! ATGATAATATCATAATTGGG+AGG - chr2.3:75935627-75935646 None:intergenic 25.0%
! CACATGTTAAAAAGAAAGTA+TGG - chr2.3:75934144-75934163 None:intergenic 25.0%
! GAAGATTGGAAATGAAAAAA+AGG + chr2.3:75933953-75933972 MS.gene002033:CDS 25.0%
! GACTAAGAGAAAGAAAAAAT+TGG + chr2.3:75934109-75934128 MS.gene002033:CDS 25.0%
! GCAAATAAAAGCAAAAAAGA+TGG + chr2.3:75933795-75933814 MS.gene002033:CDS 25.0%
! GCTATAATGTGAAATAGAAA+AGG + chr2.3:75934820-75934839 MS.gene002033:intron 25.0%
! GTTATAAAATTCATATGCCA+AGG - chr2.3:75933733-75933752 None:intergenic 25.0%
! TGATAATATCATAATTGGGA+GGG - chr2.3:75935626-75935645 None:intergenic 25.0%
! TGATCAACAAAATGCAATAT+AGG - chr2.3:75934682-75934701 None:intergenic 25.0%
! TGGATAAAAAGAGACAAAAA+AGG + chr2.3:75934078-75934097 MS.gene002033:CDS 25.0%
!! CTAATTTTATTGACAACGAA+TGG + chr2.3:75935007-75935026 MS.gene002033:intron 25.0%
!! TGTTACTTCTACTATTATGT+AGG + chr2.3:75934420-75934439 MS.gene002033:intron 25.0%
!!! CATATGTGTTTTAGATTTTG+GGG - chr2.3:75935449-75935468 None:intergenic 25.0%
!!! CATTTTGTTGATCACTTTTT+GGG + chr2.3:75934688-75934707 MS.gene002033:intron 25.0%
!!! GTCATATGTGTTTTAGATTT+TGG - chr2.3:75935451-75935470 None:intergenic 25.0%
!!! TGTTACTTTTACTACTATGT+AGG + chr2.3:75934203-75934222 MS.gene002033:intron 25.0%
!!! TTATTTTGGTAGTCTCTTTA+GGG - chr2.3:75935141-75935160 None:intergenic 25.0%
!!! TTGTTATTTTTTCTGTCTTG+CGG - chr2.3:75935555-75935574 None:intergenic 25.0%
!!! TTTATTTTGGTAGTCTCTTT+AGG - chr2.3:75935142-75935161 None:intergenic 25.0%
!!! TTTTATTTGCATATTCTCGA+CGG - chr2.3:75933785-75933804 None:intergenic 25.0%
AAAAAGTCCTGCTATAGAAA+AGG + chr2.3:75934881-75934900 MS.gene002033:intron 30.0%
ACAATTGAACATGTATGCAA+AGG + chr2.3:75935765-75935784 MS.gene002033:intron 30.0%
ACGTGATTACTTAACAAAAG+AGG + chr2.3:75933875-75933894 MS.gene002033:CDS 30.0%
AGATTAGGATCTAACACAAT+TGG + chr2.3:75934611-75934630 MS.gene002033:intron 30.0%
AGCAAAATGGTACATATAGA+TGG - chr2.3:75934803-75934822 None:intergenic 30.0%
ATAAGCAATGATGAAGTTCA+AGG - chr2.3:75935587-75935606 None:intergenic 30.0%
ATTGAAGAATTAGAATGCCA+AGG + chr2.3:75935799-75935818 MS.gene002033:CDS 30.0%
CCTAAAGGAATAATCATTAG+AGG - chr2.3:75935500-75935519 None:intergenic 30.0%
CCTCTAATGATTATTCCTTT+AGG + chr2.3:75935497-75935516 MS.gene002033:intron 30.0%
GAAACAAACCAATACAAACA+AGG + chr2.3:75935067-75935086 MS.gene002033:intron 30.0%
GAATATTACATCCAATCCAA+TGG + chr2.3:75933608-75933627 MS.gene002033:CDS 30.0%
GACAAAGAACAAAATCTTAC+CGG - chr2.3:75933459-75933478 None:intergenic 30.0%
GATAGCAGTACTAAAATTCT+AGG - chr2.3:75934167-75934186 None:intergenic 30.0%
GATTAGGATCTAACACAATT+GGG + chr2.3:75934612-75934631 MS.gene002033:intron 30.0%
GCTTAGATAGGACTAAAAAA+AGG + chr2.3:75934033-75934052 MS.gene002033:CDS 30.0%
TAAGCAATGATGAAGTTCAA+GGG - chr2.3:75935586-75935605 None:intergenic 30.0%
TAGAAAACATGAACGAGAAA+GGG + chr2.3:75935849-75935868 MS.gene002033:CDS 30.0%
TCATAATTTGAGCAAGACTT+TGG - chr2.3:75935679-75935698 None:intergenic 30.0%
TCCAAATGTCAAATGAGATT+AGG + chr2.3:75934596-75934615 MS.gene002033:intron 30.0%
TCCTAATCTCATTTGACATT+TGG - chr2.3:75934600-75934619 None:intergenic 30.0%
TGAAAAAAAGGATCAGAATG+TGG + chr2.3:75933965-75933984 MS.gene002033:CDS 30.0%
TTAGAAAACATGAACGAGAA+AGG + chr2.3:75935848-75935867 MS.gene002033:CDS 30.0%
TTGAACTTCATCATTGCTTA+TGG + chr2.3:75935586-75935605 MS.gene002033:intron 30.0%
TTGAAGAATTAGAATGCCAA+GGG + chr2.3:75935800-75935819 MS.gene002033:CDS 30.0%
! GTATGGTTATTTCACTTGAT+CGG + chr2.3:75933931-75933950 MS.gene002033:CDS 30.0%
! TATAGTAGGAAATGTGGTTT+TGG + chr2.3:75933756-75933775 MS.gene002033:CDS 30.0%
! TGGATTTTCGTGTAATGAAT+TGG - chr2.3:75934969-75934988 None:intergenic 30.0%
!! GCATTTTGTTGATCACTTTT+TGG + chr2.3:75934687-75934706 MS.gene002033:intron 30.0%
AAAAGACAGCAAAGACATGA+AGG - chr2.3:75935830-75935849 None:intergenic 35.0%
AAAATTTGTGTGCTCCAAAG+AGG + chr2.3:75933833-75933852 MS.gene002033:CDS 35.0%
AAATTTGTGTGCTCCAAAGA+GGG + chr2.3:75933834-75933853 MS.gene002033:CDS 35.0%
AACACTAGAGAATGATGCAA+AGG + chr2.3:75935174-75935193 MS.gene002033:intron 35.0%
AACGAGTATAGTAGGAAATG+TGG + chr2.3:75933750-75933769 MS.gene002033:CDS 35.0%
ATGGAAGTCTACATGAGATT+AGG + chr2.3:75934572-75934591 MS.gene002033:intron 35.0%
ATTTCACTTGATCGGAAGAT+TGG + chr2.3:75933939-75933958 MS.gene002033:CDS 35.0%
CTGTTGTATTGTTGTCCTAA+AGG - chr2.3:75935515-75935534 None:intergenic 35.0%
TAATGTACCACACTTGGAAA+TGG + chr2.3:75933692-75933711 MS.gene002033:CDS 35.0%
TAGTCCTATCTAAGCAATCT+CGG - chr2.3:75934028-75934047 None:intergenic 35.0%
TGTTAGTAATGTACCACACT+TGG + chr2.3:75933686-75933705 MS.gene002033:CDS 35.0%
TTCATCATTGCTTATGGACT+TGG + chr2.3:75935592-75935611 MS.gene002033:intron 35.0%
TTGCATCATTCTCTAGTGTT+AGG - chr2.3:75935174-75935193 None:intergenic 35.0%
! GGTTGAAGGTGTAGAAAAAT+TGG + chr2.3:75933629-75933648 MS.gene002033:CDS 35.0%
! TTTTCTACACCTTCAACCAT+TGG - chr2.3:75933627-75933646 None:intergenic 35.0%
!! CTAAAAAAAGGCATCCAACT+TGG + chr2.3:75934045-75934064 MS.gene002033:CDS 35.0%
!! TGTTCATTTTTCGGCTGTTT+TGG - chr2.3:75935211-75935230 None:intergenic 35.0%
!! TTGTCCGGATGTTCATTTTT+CGG - chr2.3:75935220-75935239 None:intergenic 35.0%
!! TTTGTTTCTTTTCGCTTGTG+CGG - chr2.3:75935055-75935074 None:intergenic 35.0%
AACAGGATGTTTAGCACGTA+TGG + chr2.3:75933914-75933933 MS.gene002033:CDS 40.0%
AATGGAAGCCAATCTGGATA+TGG + chr2.3:75934481-75934500 MS.gene002033:intron 40.0%
AATGTGGACACTGTTGATCT+TGG + chr2.3:75933981-75934000 MS.gene002033:CDS 40.0%
ACAGCCGAAAAATGAACATC+CGG + chr2.3:75935213-75935232 MS.gene002033:intron 40.0%
ACATCCAATCCAATGGTTGA+AGG + chr2.3:75933615-75933634 MS.gene002033:CDS 40.0%
ATATGGAAGCCATGCTTTGA+TGG + chr2.3:75934498-75934517 MS.gene002033:intron 40.0%
CAATCTCCTGCAAACAATGA+TGG + chr2.3:75934228-75934247 MS.gene002033:CDS 40.0%
CATCATTCTCTAGTGTTAGG+CGG - chr2.3:75935171-75935190 None:intergenic 40.0%
CCATTGTCAGCTTCTGTTAA+AGG - chr2.3:75934556-75934575 None:intergenic 40.0%
CCTTTAACAGAAGCTGACAA+TGG + chr2.3:75934553-75934572 MS.gene002033:intron 40.0%
GTCACAGTAACATATGGTGT+TGG + chr2.3:75935884-75935903 MS.gene002033:CDS 40.0%
GTGAATGATATCGTGCAACA+GGG + chr2.3:75933651-75933670 MS.gene002033:CDS 40.0%
TACACCTTCAACCATTGGAT+TGG - chr2.3:75933622-75933641 None:intergenic 40.0%
TAGGACAACAATACAACAGC+AGG + chr2.3:75935516-75935535 MS.gene002033:intron 40.0%
TCAAAGTCCATTTCCAAGTG+TGG - chr2.3:75933702-75933721 None:intergenic 40.0%
TCCAATTTCTCAAGCCAAGT+TGG - chr2.3:75934062-75934081 None:intergenic 40.0%
TGTGTACCATCATTGTTTGC+AGG - chr2.3:75934237-75934256 None:intergenic 40.0%
! AAGACATGAAGGTTTTCCCT+TGG - chr2.3:75935819-75935838 None:intergenic 40.0%
! AGCAGAACCTTTTCTATAGC+AGG - chr2.3:75934891-75934910 None:intergenic 40.0%
! GATGGTACACATGCTTTGAA+TGG + chr2.3:75934246-75934265 MS.gene002033:CDS 40.0%
! GTACACATGCTTTGAATGGA+AGG + chr2.3:75934250-75934269 MS.gene002033:CDS 40.0%
! TCCAACTTGGCTTGAGAAAT+TGG + chr2.3:75934058-75934077 MS.gene002033:CDS 40.0%
! TTGGTGTCTGCCATCAATAT+GGG - chr2.3:75935387-75935406 None:intergenic 40.0%
! TTTGAGATGCCATCAAAGCA+TGG - chr2.3:75934510-75934529 None:intergenic 40.0%
!! ATGGTGTTGGCTTCTAGAAA+GGG + chr2.3:75935897-75935916 MS.gene002033:CDS 40.0%
!! CTTTAGGGAGTGTTGTTTCA+CGG - chr2.3:75935126-75935145 None:intergenic 40.0%
!! TATGGTGTTGGCTTCTAGAA+AGG + chr2.3:75935896-75935915 MS.gene002033:CDS 40.0%
!! TTGGGTCGATGCTCTTATTT+TGG + chr2.3:75934764-75934783 MS.gene002033:intron 40.0%
!! TTTAGGGAGTGTTGTTTCAC+GGG - chr2.3:75935125-75935144 None:intergenic 40.0%
AGAGCCGAGATTGCTTAGAT+AGG + chr2.3:75934021-75934040 MS.gene002033:CDS 45.0%
CATGGCTTCCATATCCAGAT+TGG - chr2.3:75934492-75934511 None:intergenic 45.0%
CCAAGCATGTATGCCATCAT+GGG - chr2.3:75934461-75934480 None:intergenic 45.0%
CTGTATGCTACCTATGCGTA+GGG - chr2.3:75935254-75935273 None:intergenic 45.0%
GAAAGGGTAGGTTTGCAAAG+AGG + chr2.3:75935913-75935932 MS.gene002033:CDS 45.0%
GAACATCCGGACAAGAGAAA+TGG + chr2.3:75935226-75935245 MS.gene002033:intron 45.0%
GAACGAGAAAGGGCAGATTT+GGG + chr2.3:75935859-75935878 MS.gene002033:CDS 45.0%
GATGGCATACATGCTTGGAA+TGG + chr2.3:75934463-75934482 MS.gene002033:intron 45.0%
GGTGAATGATATCGTGCAAC+AGG + chr2.3:75933650-75933669 MS.gene002033:CDS 45.0%
TATTTGCTCCTGCTGTCAGT+TGG - chr2.3:75935353-75935372 None:intergenic 45.0%
TCCAAGCATGTATGCCATCA+TGG - chr2.3:75934462-75934481 None:intergenic 45.0%
TGAACGAGAAAGGGCAGATT+TGG + chr2.3:75935858-75935877 MS.gene002033:CDS 45.0%
TGGACACTGTTGATCTTGGT+GGG + chr2.3:75933985-75934004 MS.gene002033:CDS 45.0%
TGGGTCGTCACAGTAACATA+TGG + chr2.3:75935878-75935897 MS.gene002033:CDS 45.0%
TGGTGTCTGCCATCAATATG+GGG - chr2.3:75935386-75935405 None:intergenic 45.0%
TGTATGCTACCTATGCGTAG+GGG - chr2.3:75935253-75935272 None:intergenic 45.0%
TTGTTTCACGGGTTTGTGTC+GGG - chr2.3:75935114-75935133 None:intergenic 45.0%
! GGAAATGGACTTTGAGTCTG+AGG + chr2.3:75933707-75933726 MS.gene002033:CDS 45.0%
! GTTGGTGTCTGCCATCAATA+TGG - chr2.3:75935388-75935407 None:intergenic 45.0%
! GTTGTTTCACGGGTTTGTGT+CGG - chr2.3:75935115-75935134 None:intergenic 45.0%
! TACGGCACCCTTGTTTGTAT+TGG - chr2.3:75935078-75935097 None:intergenic 45.0%
! TTTCTTTTCGCTTGTGCGGT+TGG - chr2.3:75935051-75935070 None:intergenic 45.0%
!! CAAACAAGGGTGCCGTATAT+AGG + chr2.3:75935081-75935100 MS.gene002033:intron 45.0%
!! TGTTGGCTTCTAGAAAGGGT+AGG + chr2.3:75935901-75935920 MS.gene002033:CDS 45.0%
!!! GTTTTAGATTTTGGGGCGTC+TGG - chr2.3:75935442-75935461 None:intergenic 45.0%
!!! TTGTGCGGTTGGTTTTTGTG+CGG - chr2.3:75935040-75935059 None:intergenic 45.0%
AAAATCTTACCGGTGCCCAG+AGG - chr2.3:75933449-75933468 None:intergenic 50.0%
AGAAATGGACCCCTACGCAT+AGG + chr2.3:75935241-75935260 MS.gene002033:intron 50.0%
AGGGGTCCATTTCTCTTGTC+CGG - chr2.3:75935235-75935254 None:intergenic 50.0%
ATTCTCTAGTGTTAGGCGGC+GGG - chr2.3:75935167-75935186 None:intergenic 50.0%
CAATCTCCAGCAACCCATGA+TGG + chr2.3:75934445-75934464 MS.gene002033:intron 50.0%
CACCATTCATCACCACCTCT+GGG + chr2.3:75933431-75933450 MS.gene002033:CDS 50.0%
CATTCTCTAGTGTTAGGCGG+CGG - chr2.3:75935168-75935187 None:intergenic 50.0%
CCCATGATGGCATACATGCT+TGG + chr2.3:75934458-75934477 MS.gene002033:intron 50.0%
GCTCGAGCAGAAACTAGAAC+AGG + chr2.3:75933897-75933916 MS.gene002033:CDS 50.0%
GCTGTATGCTACCTATGCGT+AGG - chr2.3:75935255-75935274 None:intergenic 50.0%
GCTTGGAATGGAAGCCAATC+TGG + chr2.3:75934475-75934494 MS.gene002033:intron 50.0%
GGACACTGTTGATCTTGGTG+GGG + chr2.3:75933986-75934005 MS.gene002033:CDS 50.0%
GTGGACACTGTTGATCTTGG+TGG + chr2.3:75933984-75934003 MS.gene002033:CDS 50.0%
TCACCATTCATCACCACCTC+TGG + chr2.3:75933430-75933449 MS.gene002033:CDS 50.0%
TCGGGTGTTCCGCCTATATA+CGG - chr2.3:75935096-75935115 None:intergenic 50.0%
TGCTAACACCAACTGACAGC+AGG + chr2.3:75935342-75935361 MS.gene002033:intron 50.0%
TGTCTGCCATCAATATGGGG+CGG - chr2.3:75935383-75935402 None:intergenic 50.0%
! TGTATGCCATCATGGGTTGC+TGG - chr2.3:75934454-75934473 None:intergenic 50.0%
! TGTGCTCATTGCAAGTGCTG+CGG - chr2.3:75935416-75935435 None:intergenic 50.0%
!! ACAAGGGTGCCGTATATAGG+CGG + chr2.3:75935084-75935103 MS.gene002033:intron 50.0%
!! CATTGCAAGTGCTGCGGTTT+TGG - chr2.3:75935410-75935429 None:intergenic 50.0%
!! CTACGCGGTTTGTTGTGCAA+GGG - chr2.3:75935294-75935313 None:intergenic 50.0%
!! GAGGGCTTTGCTTTGAGATG+CGG + chr2.3:75935955-75935974 MS.gene002033:CDS 50.0%
!! TACGCGGTTTGTTGTGCAAG+GGG - chr2.3:75935293-75935312 None:intergenic 50.0%
!! TCTACGCGGTTTGTTGTGCA+AGG - chr2.3:75935295-75935314 None:intergenic 50.0%
!! TTTGCTTTGAGATGCGGCGA+CGG + chr2.3:75935961-75935980 MS.gene002033:CDS 50.0%
!!! TAGGCGGCGGGGTTTTATTT+TGG - chr2.3:75935155-75935174 None:intergenic 50.0%
CACCAACCGCCCCATATTGA+TGG + chr2.3:75935374-75935393 MS.gene002033:intron 55.0%
TGCCATCAATATGGGGCGGT+TGG - chr2.3:75935379-75935398 None:intergenic 55.0%
TGCTCCAAAGAGGGTAGACG+CGG + chr2.3:75933843-75933862 MS.gene002033:CDS 55.0%
TTCTCTAGTGTTAGGCGGCG+GGG - chr2.3:75935166-75935185 None:intergenic 55.0%
! AGGTTTGCAAAGAGGCGCGA+CGG + chr2.3:75935921-75935940 MS.gene002033:CDS 55.0%
! GGACTTTGAGTCTGAGGCCT+TGG + chr2.3:75933713-75933732 MS.gene002033:CDS 55.0%
! TGCCCAGAGGTGGTGATGAA+TGG - chr2.3:75933436-75933455 None:intergenic 55.0%
!! ACGCGGTTTGTTGTGCAAGG+GGG - chr2.3:75935292-75935311 None:intergenic 55.0%
!! GCAAGTGCTGCGGTTTTGGT+TGG - chr2.3:75935406-75935425 None:intergenic 55.0%
ATCTGTGGCGCGGTTCTACG+CGG - chr2.3:75935309-75935328 None:intergenic 60.0%
ATCTTACCGGTGCCCAGAGG+TGG - chr2.3:75933446-75933465 None:intergenic 60.0%
CAGCTGCCAGCAGCTATCTG+TGG - chr2.3:75935324-75935343 None:intergenic 60.0%
GACACCGCGTCTACCCTCTT+TGG - chr2.3:75933850-75933869 None:intergenic 60.0%
TCATCACCACCTCTGGGCAC+CGG + chr2.3:75933437-75933456 MS.gene002033:CDS 60.0%
! TTTGCAAAGAGGCGCGACGG+TGG + chr2.3:75935924-75935943 MS.gene002033:CDS 60.0%
!! GGTTTGTTGTGCAAGGGGGC+TGG - chr2.3:75935288-75935307 None:intergenic 60.0%
ACCGCGCCACAGATAGCTGC+TGG + chr2.3:75935315-75935334 MS.gene002033:intron 65.0%
GCCAGCAGCTATCTGTGGCG+CGG - chr2.3:75935319-75935338 None:intergenic 65.0%
!! GCTTTGAGATGCGGCGACGG+CGG + chr2.3:75935964-75935983 MS.gene002033:CDS 65.0%
TGCGGCGACGGCGGAAGTGA+CGG + chr2.3:75935973-75935992 MS.gene002033:CDS 70.0%
! AAGAGGCGCGACGGTGGCAG+TGG + chr2.3:75935930-75935949 MS.gene002033:CDS 70.0%
AGGCGCGACGGTGGCAGTGG+CGG + chr2.3:75935933-75935952 MS.gene002033:CDS 75.0%
GGCGACGGCGGAAGTGACGG+AGG + chr2.3:75935976-75935995 MS.gene002033:CDS 75.0%
! GCGACGGTGGCAGTGGCGGA+GGG + chr2.3:75935937-75935956 MS.gene002033:CDS 75.0%
! CGCGACGGTGGCAGTGGCGG+AGG + chr2.3:75935936-75935955 MS.gene002033:CDS 80.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 75933392 75936005 75933392 ID=MS.gene002033
chr2.3 mRNA 75933392 75936005 75933392 ID=MS.gene002033.t1;Parent=MS.gene002033
chr2.3 exon 75933392 75933458 75933392 ID=MS.gene002033.t1.exon1;Parent=MS.gene002033.t1
chr2.3 CDS 75933392 75933458 75933392 ID=cds.MS.gene002033.t1;Parent=MS.gene002033.t1
chr2.3 exon 75933601 75934130 75933601 ID=MS.gene002033.t1.exon2;Parent=MS.gene002033.t1
chr2.3 CDS 75933601 75934130 75933601 ID=cds.MS.gene002033.t1;Parent=MS.gene002033.t1
chr2.3 exon 75934225 75934290 75934225 ID=MS.gene002033.t1.exon3;Parent=MS.gene002033.t1
chr2.3 CDS 75934225 75934290 75934225 ID=cds.MS.gene002033.t1;Parent=MS.gene002033.t1
chr2.3 exon 75935787 75936005 75935787 ID=MS.gene002033.t1.exon4;Parent=MS.gene002033.t1
chr2.3 CDS 75935787 75936005 75935787 ID=cds.MS.gene002033.t1;Parent=MS.gene002033.t1
Gene Sequence

>MS.gene002033

ATGGTAGAAACGACACACGTAGAACATGATGCAAATCTTCACCATTCATCACCACCTCTGGGCACCGGTAAGATTTTGTTCTTTGTCGATGAATATACACACGTAGAACATGAATATTTGAAAGTATTTGGACAATATAAAATTAATATAAAATGTCTATGAATATTAACATGTTGACTTTATATTTTTTTTTTCTTTCCATTTTCTAGATAATGTGAATATTACATCCAATCCAATGGTTGAAGGTGTAGAAAAATTGGTGAATGATATCGTGCAACAGGGAAAAGAGAGTACTGTTAGTAATGTACCACACTTGGAAATGGACTTTGAGTCTGAGGCCTTGGCATATGAATTTTATAACGAGTATAGTAGGAAATGTGGTTTTGGTATCCGTCGAGAATATGCAAATAAAAGCAAAAAAGATGGAGTTCTTACTTCAAGAAAATTTGTGTGCTCCAAAGAGGGTAGACGCGGTGTCGACAAACGTGATTACTTAACAAAAGAGGCTCGAGCAGAAACTAGAACAGGATGTTTAGCACGTATGGTTATTTCACTTGATCGGAAGATTGGAAATGAAAAAAAGGATCAGAATGTGGACACTGTTGATCTTGGTGGGGCAAAAGCAATAAAGAGCCGAGATTGCTTAGATAGGACTAAAAAAAGGCATCCAACTTGGCTTGAGAAATTGGATAAAAAGAGACAAAAAAGGCTATCACAGACTAAGAGAAAGAAAAAATTGGTAAACAACTCTTTACTTTCTTTTTAACATGTGCCTAGAATTTTAGTACTGCTATCACATATACATAATACATGTTACTTTTACTACTATGTAGGAACAATCTCCTGCAAACAATGATGGTACACATGCTTTGAATGGAAGGAAGAGAAAGAAAAAATTGGTAAAAATATCCATACTTTCTTTTTAACATGTGACAGAATTTCAGTACTGCTATCACATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAATACATGTTACTTCTACTATTATGTAGGAACAATCTCCAGCAACCCATGATGGCATACATGCTTGGAATGGAAGCCAATCTGGATATGGAAGCCATGCTTTGATGGCATCTCAAACTGAGAATGCAAGTCGACATTTACCTTTAACAGAAGCTGACAATGGAAGTCTACATGAGATTAGGATCCAAATGTCAAATGAGATTAGGATCTAACACAATTGGGATGTATAACTTTTTTTAGACATTACTACTTTTTCTATTAGGGGACCTATATTGCATTTTGTTGATCACTTTTTGGGTTTGATGACAATTCTGATATACTAGTTTATTTTGGCTACTTTTATCTTATAATTTGGGTCGATGCTCTTATTTTGGCTACTTTTCATTTCCATCTATATGTACCATTTTGCTATAATGTGAAATAGAAAAGGTTCTGCTATAGTTACACTTGAAAAAAAATGTGAAATAGAAAAAGTCCTGCTATAGAAAAGGTTCTGCTATAGTTACACTGTTAAAAAAAATATGAATATGGTACATTTGATAACAGTACAAAGCCAATTCATTACACGAAAATCCAAACATTACATTTGATATGCTAATTTTATTGACAACGAATGGGTGCAAGCCGCACAAAAACCAACCGCACAAGCGAAAAGAAACAAACCAATACAAACAAGGGTGCCGTATATAGGCGGAACACCCGACACAAACCCGTGAAACAACACTCCCTAAAGAGACTACCAAAATAAAACCCCGCCGCCTAACACTAGAGAATGATGCAAAGGTATAAACTGAACCAAAACAGCCGAAAAATGAACATCCGGACAAGAGAAATGGACCCCTACGCATAGGTAGCATACAGCTGCATCAAAACCAGCCCCCTTGCACAACAAACCGCGTAGAACCGCGCCACAGATAGCTGCTGGCAGCTGCTAACACCAACTGACAGCAGGAGCAAATAGCACCAACCGCCCCATATTGATGGCAGACACCAACCAAAACCGCAGCACTTGCAATGAGCACAAAACCAGACGCCCCAAAATCTAAAACACATATGACAATACAATTCAATACAATGTTGGGCTCCTCTAATGATTATTCCTTTAGGACAACAATACAACAGCAGGACATAAAAACAGTCCGCAAGACAGAAAAAATAACAACATTTTATCCCTTGAACTTCATCATTGCTTATGGACTTGGCTCAGTAACCCTCCCAATTATGATATTATCATATATGTATCAGCATTACATGTAACAGCAACCAAAGTCTTGCTCAAATTATGAATATCATCACTAGCAAAATCTGCAAAAAGAATAAAATACAAAGTGAGTTCCTCTAAATTGATATGAACAATTGAACATGTATGCAAAGGTATCTGTCAAAATTGAAGAATTAGAATGCCAAGGGAAAACCTTCATGTCTTTGCTGTCTTTTAGAAAACATGAACGAGAAAGGGCAGATTTGGGTCGTCACAGTAACATATGGTGTTGGCTTCTAGAAAGGGTAGGTTTGCAAAGAGGCGCGACGGTGGCAGTGGCGGAGGGCTTTGCTTTGAGATGCGGCGACGGCGGAAGTGACGGAGGTCGTTAA
Protein sequence

>MS.gene002033.t1

MVETTHVEHDANLHHSSPPLGTDNVNITSNPMVEGVEKLVNDIVQQGKESTVSNVPHLEMDFESEALAYEFYNEYSRKCGFGIRREYANKSKKDGVLTSRKFVCSKEGRRGVDKRDYLTKEARAETRTGCLARMVISLDRKIGNEKKDQNVDTVDLGGAKAIKSRDCLDRTKKRHPTWLEKLDKKRQKRLSQTKRKKKLEQSPANNDGTHALNGRKRKKKLVSVKIEELECQGKTFMSLLSFRKHERERADLGRHSNIWCWLLERVGLQRGATVAVAEGFALRCGDGGSDGGR