AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002063

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002063.t1 MTR_2g006380 86.810 326 35 4 1 321 1 323 0.0 564
MS.gene002063.t1 MTR_2g006270 70.186 322 82 7 10 321 4 321 1.40e-153 439
MS.gene002063.t1 MTR_1g058210 47.826 322 143 10 12 316 5 318 2.70e-85 265
MS.gene002063.t1 MTR_2g024430 47.649 319 143 11 12 316 13 321 2.14e-81 256
MS.gene002063.t1 MTR_5g040880 43.218 317 163 8 10 316 2 311 3.55e-80 253
MS.gene002063.t1 MTR_5g061220 44.011 359 148 13 13 330 13 359 1.24e-79 252
MS.gene002063.t1 MTR_5g040740 44.880 332 160 11 11 329 4 325 4.32e-78 244
MS.gene002063.t1 MTR_5g060780 41.667 324 155 12 13 316 6 315 2.55e-71 227
MS.gene002063.t1 MTR_5g061890 41.975 324 154 12 13 316 6 315 2.01e-70 224
MS.gene002063.t1 MTR_5g460920 41.667 324 155 12 13 316 6 315 2.41e-70 224
MS.gene002063.t1 MTR_5g060630 41.595 351 154 12 12 316 5 350 4.95e-69 224
MS.gene002063.t1 MTR_5g460740 41.595 351 154 12 12 316 5 350 4.95e-69 224
MS.gene002063.t1 MTR_5g082140 43.260 319 162 10 11 316 4 316 2.37e-68 221
MS.gene002063.t1 MTR_3g073420 39.106 358 154 12 14 317 14 361 8.18e-68 223
MS.gene002063.t1 MTR_2g094570 33.676 389 182 14 3 328 46 421 4.15e-56 194
MS.gene002063.t1 MTR_2g094570 34.232 371 173 13 16 328 8 365 5.81e-56 193
MS.gene002063.t1 MTR_2g094570 34.232 371 173 13 16 328 8 365 6.28e-56 192
MS.gene002063.t1 MTR_5g046120 80.556 108 19 1 64 169 16 123 7.99e-55 176
MS.gene002063.t1 MTR_1g064430 32.418 364 175 11 16 316 24 379 4.26e-51 180
MS.gene002063.t1 MTR_1g064430 32.418 364 175 11 16 316 37 392 4.59e-51 180
MS.gene002063.t1 MTR_1g094960 33.521 355 168 11 19 316 19 362 4.94e-50 176
MS.gene002063.t1 MTR_4g058930 32.086 374 177 14 9 317 2 363 8.96e-49 173
MS.gene002063.t1 MTR_4g058930 32.086 374 177 14 9 317 2 363 1.17e-48 173
MS.gene002063.t1 MTR_5g062970 44.608 204 102 4 19 218 38 234 6.89e-48 161
MS.gene002063.t1 MTR_7g101275 35.443 316 173 12 29 323 2 307 1.20e-46 162
MS.gene002063.t1 MTR_0041s0120 32.121 330 177 12 29 323 2 319 9.25e-40 145
MS.gene002063.t1 MTR_7g101280 36.321 212 122 5 16 222 28 231 8.64e-35 134
MS.gene002063.t1 MTR_0041s0110 36.321 212 122 5 16 222 28 231 8.64e-35 134
MS.gene002063.t1 MTR_4g124900 31.193 327 188 12 16 316 20 335 2.21e-34 134
MS.gene002063.t1 MTR_4g124900 31.193 327 188 12 16 316 20 335 2.58e-34 133
MS.gene002063.t1 MTR_1g024025 29.938 324 195 10 16 315 40 355 4.33e-34 133
MS.gene002063.t1 MTR_5g060770 37.698 252 106 9 73 315 9 218 3.48e-33 126
MS.gene002063.t1 MTR_5g076270 28.972 321 205 9 2 305 7 321 9.62e-33 129
MS.gene002063.t1 MTR_8g101360 30.275 327 193 11 16 316 28 345 3.32e-32 127
MS.gene002063.t1 MTR_2g018690 30.333 300 192 7 19 304 23 319 4.81e-32 127
MS.gene002063.t1 MTR_3g064050 29.355 310 190 9 16 305 23 323 5.08e-32 126
MS.gene002063.t1 MTR_2g005240 29.936 314 193 12 16 311 20 324 8.79e-32 125
MS.gene002063.t1 MTR_8g079492 30.897 301 190 8 19 305 23 319 1.65e-31 125
MS.gene002063.t1 MTR_4g021580 29.582 311 191 10 19 311 19 319 1.74e-31 124
MS.gene002063.t1 MTR_8g079492 30.897 301 190 8 19 305 23 319 1.80e-31 125
MS.gene002063.t1 MTR_2g014770 27.885 312 186 10 19 305 53 350 2.21e-31 124
MS.gene002063.t1 MTR_4g021580 29.582 311 191 10 19 311 19 319 2.29e-31 124
MS.gene002063.t1 MTR_2g014770 27.885 312 186 10 19 305 53 350 3.05e-31 124
MS.gene002063.t1 MTR_2g043250 30.247 324 195 11 16 316 20 335 3.97e-31 124
MS.gene002063.t1 MTR_8g100050 29.412 323 193 11 19 316 59 371 3.98e-31 124
MS.gene002063.t1 MTR_8g100050 29.412 323 193 11 19 316 59 371 5.63e-31 123
MS.gene002063.t1 MTR_2g093740 33.219 292 167 10 19 288 56 341 5.96e-31 123
MS.gene002063.t1 MTR_2g093740 33.219 292 167 10 19 288 56 341 6.66e-31 123
MS.gene002063.t1 MTR_2g093740 33.219 292 167 10 19 288 56 341 8.41e-31 123
MS.gene002063.t1 MTR_8g027440 27.554 323 211 9 4 311 14 328 3.76e-29 118
MS.gene002063.t1 MTR_2g093740 31.803 305 167 10 19 288 56 354 4.74e-29 117
MS.gene002063.t1 MTR_2g093740 31.803 305 167 10 19 288 56 354 5.77e-29 117
MS.gene002063.t1 MTR_2g093740 31.803 305 167 10 19 288 56 354 7.61e-29 117
MS.gene002063.t1 MTR_7g062540 29.617 287 178 9 15 287 10 286 1.92e-28 116
MS.gene002063.t1 MTR_4g088210 29.703 303 171 10 14 288 33 321 2.55e-28 115
MS.gene002063.t1 MTR_4g088210 29.703 303 171 10 14 288 33 321 3.54e-28 115
MS.gene002063.t1 MTR_4g060460 30.921 304 174 11 19 294 54 349 5.23e-26 108
MS.gene002063.t1 MTR_4g060460 30.921 304 174 11 19 294 54 349 6.16e-26 108
MS.gene002063.t1 MTR_8g079492 31.513 238 146 7 82 305 3 237 3.69e-23 100
MS.gene002063.t1 MTR_5g460910 30.566 265 110 10 60 315 14 213 6.52e-18 83.6
MS.gene002063.t1 MTR_2g093740 32.547 212 114 8 104 288 2 211 1.24e-17 84.0
MS.gene002063.t1 MTR_2g093740 32.547 212 114 8 104 288 2 211 1.32e-17 84.0
MS.gene002063.t1 MTR_8g446900 26.316 247 156 9 72 301 13 250 6.78e-16 78.2
MS.gene002063.t1 MTR_2g093740 30.667 225 114 8 104 288 2 224 9.08e-16 78.6
MS.gene002063.t1 MTR_2g093740 30.667 225 114 8 104 288 2 224 1.04e-15 78.6
MS.gene002063.t1 MTR_5g062960 46.067 89 37 5 238 316 1 88 1.16e-12 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002063.t1 AT1G77850 36.047 344 164 10 15 312 15 348 1.17e-59 201
MS.gene002063.t1 AT1G77850 36.047 344 164 10 15 312 15 348 1.40e-59 201
MS.gene002063.t1 AT4G30080 33.520 358 173 10 16 317 16 364 4.87e-52 182
MS.gene002063.t1 AT2G28350 31.903 373 176 14 11 317 2 362 9.82e-47 167
MS.gene002063.t1 AT2G28350 31.903 373 176 14 11 317 2 362 1.03e-46 168
MS.gene002063.t1 AT4G23980 30.449 312 197 8 15 312 8 313 8.31e-38 142
MS.gene002063.t1 AT4G23980 30.449 312 197 8 15 312 8 313 1.10e-37 142
MS.gene002063.t1 AT2G33860 31.935 310 176 10 19 305 54 351 2.64e-35 135
MS.gene002063.t1 AT1G35540 31.429 315 187 11 14 312 22 323 6.15e-35 134
MS.gene002063.t1 AT1G34390 30.573 314 189 10 14 312 22 321 4.76e-34 132
MS.gene002063.t1 AT1G34310 31.429 315 187 11 14 312 22 323 4.94e-34 132
MS.gene002063.t1 AT1G35520 31.210 314 189 10 14 312 22 323 1.56e-33 130
MS.gene002063.t1 AT5G37020 29.283 321 202 10 2 305 7 319 4.39e-33 129
MS.gene002063.t1 AT5G37020 29.283 321 202 10 2 305 7 319 4.84e-33 129
MS.gene002063.t1 AT5G20730 30.488 328 192 11 15 316 20 337 2.47e-32 127
MS.gene002063.t1 AT5G20730 30.488 328 192 11 15 316 20 337 2.86e-32 127
MS.gene002063.t1 AT2G46530 28.617 311 201 8 15 311 38 341 4.39e-32 126
MS.gene002063.t1 AT2G46530 28.617 311 201 8 15 311 17 320 5.47e-32 126
MS.gene002063.t1 AT5G20730 30.488 328 191 12 15 316 20 336 1.06e-31 126
MS.gene002063.t1 AT1G35240 29.936 314 193 10 14 312 22 323 1.55e-31 124
MS.gene002063.t1 AT1G30330 30.195 308 188 8 16 304 22 321 4.01e-31 124
MS.gene002063.t1 AT1G30330 30.195 308 188 8 16 304 22 321 4.01e-31 124
MS.gene002063.t1 AT1G30330 30.195 308 188 8 16 304 20 319 4.12e-31 124
MS.gene002063.t1 AT1G19850 28.986 345 203 11 1 316 26 357 6.37e-31 123
MS.gene002063.t1 AT1G19850 28.986 345 203 11 1 316 37 368 7.52e-31 123
MS.gene002063.t1 AT1G34410 29.936 314 193 10 14 312 22 323 3.12e-30 121
MS.gene002063.t1 AT3G61830 29.206 315 194 11 15 311 21 324 5.16e-30 120
MS.gene002063.t1 AT5G62000 28.221 326 199 11 16 316 58 373 5.19e-30 120
MS.gene002063.t1 AT1G19220 29.358 327 194 12 16 316 20 335 5.20e-30 121
MS.gene002063.t1 AT5G62000 28.483 323 196 11 19 316 61 373 6.18e-30 120
MS.gene002063.t1 AT5G62000 28.483 323 196 11 19 316 61 373 6.18e-30 120
MS.gene002063.t1 AT5G62000 28.483 323 196 11 19 316 61 373 6.18e-30 120
MS.gene002063.t1 AT5G62000 28.483 323 196 11 19 316 61 373 6.18e-30 120
MS.gene002063.t1 AT5G60450 32.365 241 139 8 14 239 36 267 1.57e-28 116
MS.gene002063.t1 AT1G34170 28.707 317 195 12 14 312 20 323 2.15e-28 115
MS.gene002063.t1 AT1G34170 29.000 300 170 12 14 280 20 309 3.66e-28 114
MS.gene002063.t1 AT5G60450 32.218 239 138 8 16 239 62 291 5.10e-28 115
MS.gene002063.t1 AT1G34170 29.000 300 170 12 14 280 20 309 9.16e-28 113
MS.gene002063.t1 AT1G59750 27.848 316 191 11 19 311 22 323 1.80e-27 113
MS.gene002063.t1 AT1G59750 27.848 316 191 11 19 311 22 323 2.01e-27 113
MS.gene002063.t1 AT1G59750 27.848 316 191 11 19 311 22 323 2.06e-27 113
MS.gene002063.t1 AT1G59750 27.848 316 191 11 19 311 22 323 2.06e-27 113
MS.gene002063.t1 AT1G43950 32.258 217 122 7 14 221 22 222 3.98e-27 106
MS.gene002063.t1 AT2G33860 32.487 197 113 7 125 305 2 194 9.02e-21 92.8
MS.gene002063.t1 AT3G61830 28.405 257 163 8 69 311 24 273 7.92e-20 90.5
MS.gene002063.t1 AT5G37020 31.122 196 118 7 125 305 31 224 2.55e-19 89.4
MS.gene002063.t1 AT2G46530 29.268 205 127 7 121 311 33 233 6.66e-15 75.9

Find 90 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGATGCAACTTTGGTATT+TGG 0.157694 2.3:+75693084 None:intergenic
TTCATCTTAACTCTCATTCT+AGG 0.234993 2.3:+75692330 None:intergenic
TATCTCGTACGTCTAATTTA+TGG 0.240815 2.3:-75692260 MS.gene002063:CDS
CTCATCATCGTCGTTGCAAT+TGG 0.314881 2.3:+75692833 None:intergenic
GTAGAGATTGTCCCTTGATA+TGG 0.324016 2.3:+75692285 None:intergenic
AACGACGATGATGAGGTAAT+TGG 0.328138 2.3:-75692825 MS.gene002063:CDS
GCTGCTGTTGGTGCTGGTGC+TGG 0.335243 2.3:+75693159 None:intergenic
GTATGCTTGCTTACATTAGT+AGG 0.336336 2.3:+75692780 None:intergenic
GCATCAACATCATTGGTTAT+TGG 0.339316 2.3:+75692882 None:intergenic
TTAACAAACGAACTCCATTC+AGG 0.339363 2.3:+75692606 None:intergenic
CGCATGTCCACCCTCAAATC+TGG 0.363208 2.3:-75693130 MS.gene002063:CDS
CAACGGGAAAATTATTTGTT+GGG 0.375166 2.3:-75692536 MS.gene002063:CDS
ATGGTTGCTGCTGTTGGTGC+TGG 0.385990 2.3:+75693153 None:intergenic
CAAATCTGGCAGACATTAAC+CGG 0.388348 2.3:-75693116 MS.gene002063:CDS
TCATTGTGAAGCAAAATAAA+CGG 0.393817 2.3:+75692128 None:intergenic
TAAACTCTTGGATGCAACTT+TGG 0.401395 2.3:+75693077 None:intergenic
TCAACGGGAAAATTATTTGT+TGG 0.404341 2.3:-75692537 MS.gene002063:CDS
CTGACGTTCATGGTGAAGTC+TGG 0.406535 2.3:-75692680 MS.gene002063:CDS
CTCCTCGTCACTGACGTTCA+TGG 0.407231 2.3:-75692690 MS.gene002063:CDS
AATGGAGTTCGTTTGTTAAG+AGG 0.407313 2.3:-75692602 MS.gene002063:CDS
CATTGTGAAGCAAAATAAAC+GGG 0.416220 2.3:+75692129 None:intergenic
TAATTTATGGCGCATGCTAC+AGG 0.422222 2.3:-75692247 MS.gene002063:intron
TGAAGACTCATAAATCTTCA+AGG 0.428391 2.3:-75692311 MS.gene002063:CDS
TGTGGAAAATAGTAAACTCT+TGG 0.431091 2.3:+75693065 None:intergenic
TGAGTTGGAGAAGGCAGTAA+TGG 0.441158 2.3:-75692478 MS.gene002063:CDS
ACTATCCTCGAGGTGATGAT+TGG 0.444363 2.3:-75692410 MS.gene002063:CDS
GACACAGGCATCAACATCAT+TGG 0.444932 2.3:+75692875 None:intergenic
GTTAATGTCTGCCAGATTTG+AGG 0.445255 2.3:+75693119 None:intergenic
TCATCTTAACTCTCATTCTA+GGG 0.445548 2.3:+75692331 None:intergenic
GATATAAAACACGTTTCTCT+TGG 0.446560 2.3:+75692629 None:intergenic
AGGTAATTGGTTCGTTTACA+AGG 0.453827 2.3:-75692812 MS.gene002063:CDS
TGGGAACCTCATGAGGGTCT+TGG 0.457592 2.3:+75692853 None:intergenic
AATTATTTGTTGGGATTCGC+AGG 0.462606 2.3:-75692527 MS.gene002063:CDS
GGATAGTAAACAATTTCAAA+TGG 0.472676 2.3:+75692426 None:intergenic
AGTAATGGAGGCAGTGAAGT+TGG 0.472794 2.3:-75692463 MS.gene002063:CDS
TTAATGTCTGCCAGATTTGA+GGG 0.479203 2.3:+75693120 None:intergenic
ATGAATCAATGAAAATTCAA+TGG 0.482116 2.3:-75692356 MS.gene002063:CDS
GGAGTGAATGTAAGCATTGG+AGG 0.486006 2.3:+75693013 None:intergenic
TCTCTTGGCAAACCCGTGGC+AGG 0.490609 2.3:+75692644 None:intergenic
TCATCATCGTCGTTGCAATT+GGG 0.492508 2.3:+75692834 None:intergenic
GAAAATATCTTCCCTCCGCT+TGG 0.492876 2.3:-75692732 MS.gene002063:CDS
TAGAGATTGTCCCTTGATAT+GGG 0.494859 2.3:+75692286 None:intergenic
CTCATGAGGGTCTTGGACAC+AGG 0.498403 2.3:+75692860 None:intergenic
TATGCTTGCTTACATTAGTA+GGG 0.498565 2.3:+75692781 None:intergenic
TGGTATTTGGACAGCAGTGC+CGG 0.499421 2.3:+75693097 None:intergenic
TGGCAAACCCGTGGCAGGCA+TGG 0.500790 2.3:+75692649 None:intergenic
AAGTTTCACCATGCCTGCCA+CGG 0.506565 2.3:-75692657 MS.gene002063:CDS
TAACAAACGAACTCCATTCA+GGG 0.512751 2.3:+75692607 None:intergenic
TCTTCAAGGATCCCATATCA+AGG 0.512818 2.3:-75692297 MS.gene002063:CDS
ATGTAAGCATTGGAGGAGGA+AGG 0.516472 2.3:+75693020 None:intergenic
AGTTTCACCATGCCTGCCAC+GGG 0.517459 2.3:-75692656 MS.gene002063:CDS
CATGCGATGGTTGCTGCTGT+TGG 0.533873 2.3:+75693147 None:intergenic
TTGCAATTGGGAACCTCATG+AGG 0.534875 2.3:+75692846 None:intergenic
GGAGGCAGTGAAGTTGGCAG+AGG 0.547497 2.3:-75692457 MS.gene002063:CDS
TATCTTCCCTCCGCTTGGCA+TGG 0.558368 2.3:-75692727 MS.gene002063:CDS
CGCCGATCCTCACACTGATG+AGG 0.560935 2.3:-75692928 MS.gene002063:CDS
AAGGGAGTGAATGTAAGCAT+TGG 0.561164 2.3:+75693010 None:intergenic
CTGGTGCTGGTTGAGAGACA+TGG 0.567756 2.3:+75693172 None:intergenic
GCATATTCTAAGTGGCCTTG+TGG 0.574209 2.3:+75693047 None:intergenic
GTGAATGTAAGCATTGGAGG+AGG 0.588009 2.3:+75693016 None:intergenic
GTTGGAGAAGGCAGTAATGG+AGG 0.592416 2.3:-75692475 MS.gene002063:CDS
TGGGATTCGCAGGAAAGCCG+CGG 0.593939 2.3:-75692517 MS.gene002063:CDS
GGAGCAAAAGAAAGATGAGT+TGG 0.594103 2.3:-75692493 MS.gene002063:CDS
GACCTCATCAGTGTGAGGAT+CGG 0.595759 2.3:+75692926 None:intergenic
AAAGAAAGATGAGTTGGAGA+AGG 0.605720 2.3:-75692487 MS.gene002063:CDS
AAATCTCCATGCCAAGCGGA+GGG 0.609417 2.3:+75692721 None:intergenic
CTTCAAGGATCCCATATCAA+GGG 0.609531 2.3:-75692296 MS.gene002063:CDS
GAGATGATACAGATAGTGAA+AGG 0.609814 2.3:+75692966 None:intergenic
GAAATCTCCATGCCAAGCGG+AGG 0.617181 2.3:+75692720 None:intergenic
CACCATGAACGTCAGTGACG+AGG 0.617900 2.3:+75692688 None:intergenic
CGTTTCTCTTGGCAAACCCG+TGG 0.618791 2.3:+75692640 None:intergenic
AAAACGTTGAAGATCATGAA+GGG 0.620045 2.3:+75692992 None:intergenic
CACTATCTGTATCATCTCCG+CGG 0.622122 2.3:-75692961 MS.gene002063:CDS
AAGGAGAAGCATATTCTAAG+TGG 0.626940 2.3:+75693039 None:intergenic
GAAAACGTTGAAGATCATGA+AGG 0.628457 2.3:+75692991 None:intergenic
CAATTGCAACGACGATGATG+AGG 0.630770 2.3:-75692832 MS.gene002063:CDS
GAAATTGTTTACTATCCTCG+AGG 0.641156 2.3:-75692420 MS.gene002063:CDS
TGCAATTGGGAACCTCATGA+GGG 0.643730 2.3:+75692847 None:intergenic
ATTTGAGGGTGGACATGCGA+TGG 0.643879 2.3:+75693134 None:intergenic
ATGCTTGCTTACATTAGTAG+GGG 0.652528 2.3:+75692782 None:intergenic
CATGAACGTCAGTGACGAGG+AGG 0.677277 2.3:+75692691 None:intergenic
GCGAAGACCTCATCAGTGTG+AGG 0.682334 2.3:+75692921 None:intergenic
ATGTCTGCCAGATTTGAGGG+TGG 0.682807 2.3:+75693123 None:intergenic
ATCGGCGAGGAGATCAACCG+CGG 0.684227 2.3:+75692944 None:intergenic
TGAGAAATCTCCATGCCAAG+CGG 0.686661 2.3:+75692717 None:intergenic
CTGTGTCCAAGACCCTCATG+AGG 0.688345 2.3:-75692859 MS.gene002063:CDS
GATTCGCAGGAAAGCCGCGG+CGG 0.692782 2.3:-75692514 MS.gene002063:CDS
TCATGCCAATCATCACCTCG+AGG 0.713899 2.3:+75692405 None:intergenic
AAGAGGAAGAAACTCGACGT+CGG 0.718798 2.3:-75692585 MS.gene002063:CDS
CATCAGTGTGAGGATCGGCG+AGG 0.752555 2.3:+75692931 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTCTTCTTTTTTTTTTT+TGG - chr2.3:75693067-75693086 MS.gene002063:CDS 10.0%
!! ATGAATCAATGAAAATTCAA+TGG - chr2.3:75692932-75692951 MS.gene002063:CDS 20.0%
! GGATAGTAAACAATTTCAAA+TGG + chr2.3:75692865-75692884 None:intergenic 25.0%
! TCATTGTGAAGCAAAATAAA+CGG + chr2.3:75693163-75693182 None:intergenic 25.0%
!! TATGAAGTGTCATCTAATTA+TGG + chr2.3:75693135-75693154 None:intergenic 25.0%
!! TGTTTTCATGAAAAACTCAA+CGG - chr2.3:75692735-75692754 MS.gene002063:CDS 25.0%
AAAACGTTGAAGATCATGAA+GGG + chr2.3:75692299-75692318 None:intergenic 30.0%
CAACGGGAAAATTATTTGTT+GGG - chr2.3:75692752-75692771 MS.gene002063:CDS 30.0%
CATTGTGAAGCAAAATAAAC+GGG + chr2.3:75693162-75693181 None:intergenic 30.0%
GATATAAAACACGTTTCTCT+TGG + chr2.3:75692662-75692681 None:intergenic 30.0%
TATCTCGTACGTCTAATTTA+TGG - chr2.3:75693028-75693047 MS.gene002063:CDS 30.0%
TATGCTTGCTTACATTAGTA+GGG + chr2.3:75692510-75692529 None:intergenic 30.0%
TCAACGGGAAAATTATTTGT+TGG - chr2.3:75692751-75692770 MS.gene002063:CDS 30.0%
TCATCTTAACTCTCATTCTA+GGG + chr2.3:75692960-75692979 None:intergenic 30.0%
TGAAGACTCATAAATCTTCA+AGG - chr2.3:75692977-75692996 MS.gene002063:CDS 30.0%
TGTGGAAAATAGTAAACTCT+TGG + chr2.3:75692226-75692245 None:intergenic 30.0%
TTCATCTTAACTCTCATTCT+AGG + chr2.3:75692961-75692980 None:intergenic 30.0%
! AGAGTTTACTATTTTCCACA+AGG - chr2.3:75692226-75692245 MS.gene002063:intron 30.0%
! AGCATACATTTTACATACCT+CGG - chr2.3:75692524-75692543 MS.gene002063:CDS 30.0%
! GTTTTCATGAAAAACTCAAC+GGG - chr2.3:75692736-75692755 MS.gene002063:CDS 30.0%
ATGCTTGCTTACATTAGTAG+GGG + chr2.3:75692509-75692528 None:intergenic 35.0%
GAAAACGTTGAAGATCATGA+AGG + chr2.3:75692300-75692319 None:intergenic 35.0%
GAAATTGTTTACTATCCTCG+AGG - chr2.3:75692868-75692887 MS.gene002063:CDS 35.0%
GAGATGATACAGATAGTGAA+AGG + chr2.3:75692325-75692344 None:intergenic 35.0%
GTATGCTTGCTTACATTAGT+AGG + chr2.3:75692511-75692530 None:intergenic 35.0%
TAAACTCTTGGATGCAACTT+TGG + chr2.3:75692214-75692233 None:intergenic 35.0%
TAACAAACGAACTCCATTCA+GGG + chr2.3:75692684-75692703 None:intergenic 35.0%
TAGAGATTGTCCCTTGATAT+GGG + chr2.3:75693005-75693024 None:intergenic 35.0%
TTAACAAACGAACTCCATTC+AGG + chr2.3:75692685-75692704 None:intergenic 35.0%
TTAATGTCTGCCAGATTTGA+GGG + chr2.3:75692171-75692190 None:intergenic 35.0%
TTGGATGCAACTTTGGTATT+TGG + chr2.3:75692207-75692226 None:intergenic 35.0%
! AAAGAAAGATGAGTTGGAGA+AGG - chr2.3:75692801-75692820 MS.gene002063:CDS 35.0%
! AAGGAGAAGCATATTCTAAG+TGG + chr2.3:75692252-75692271 None:intergenic 35.0%
! AATGGAGTTCGTTTGTTAAG+AGG - chr2.3:75692686-75692705 MS.gene002063:CDS 35.0%
! AATTATTTGTTGGGATTCGC+AGG - chr2.3:75692761-75692780 MS.gene002063:CDS 35.0%
! ACGTGTTTTATATCCCTGAA+TGG - chr2.3:75692668-75692687 MS.gene002063:CDS 35.0%
! ATATTTTCAGCACAAAACCG+AGG + chr2.3:75692544-75692563 None:intergenic 35.0%
! GCATCAACATCATTGGTTAT+TGG + chr2.3:75692409-75692428 None:intergenic 35.0%
! TGATTGGCATGATTTTGTAG+TGG - chr2.3:75692894-75692913 MS.gene002063:CDS 35.0%
!! AGGTAATTGGTTCGTTTACA+AGG - chr2.3:75692476-75692495 MS.gene002063:CDS 35.0%
AACGACGATGATGAGGTAAT+TGG - chr2.3:75692463-75692482 MS.gene002063:CDS 40.0%
AAGGGAGTGAATGTAAGCAT+TGG + chr2.3:75692281-75692300 None:intergenic 40.0%
CAAATCTGGCAGACATTAAC+CGG - chr2.3:75692172-75692191 MS.gene002063:intron 40.0%
CTTCAAGGATCCCATATCAA+GGG - chr2.3:75692992-75693011 MS.gene002063:CDS 40.0%
GGAGCAAAAGAAAGATGAGT+TGG - chr2.3:75692795-75692814 MS.gene002063:CDS 40.0%
GTAGAGATTGTCCCTTGATA+TGG + chr2.3:75693006-75693025 None:intergenic 40.0%
GTTAATGTCTGCCAGATTTG+AGG + chr2.3:75692172-75692191 None:intergenic 40.0%
TAATTTATGGCGCATGCTAC+AGG - chr2.3:75693041-75693060 MS.gene002063:CDS 40.0%
TCATCATCGTCGTTGCAATT+GGG + chr2.3:75692457-75692476 None:intergenic 40.0%
TCTTCAAGGATCCCATATCA+AGG - chr2.3:75692991-75693010 MS.gene002063:CDS 40.0%
AAGAGGAAGAAACTCGACGT+CGG - chr2.3:75692703-75692722 MS.gene002063:CDS 45.0%
ACTATCCTCGAGGTGATGAT+TGG - chr2.3:75692878-75692897 MS.gene002063:CDS 45.0%
CAATTGCAACGACGATGATG+AGG - chr2.3:75692456-75692475 MS.gene002063:CDS 45.0%
CACTATCTGTATCATCTCCG+CGG - chr2.3:75692327-75692346 MS.gene002063:CDS 45.0%
CTCATCATCGTCGTTGCAAT+TGG + chr2.3:75692458-75692477 None:intergenic 45.0%
GAAAATATCTTCCCTCCGCT+TGG - chr2.3:75692556-75692575 MS.gene002063:CDS 45.0%
GACACAGGCATCAACATCAT+TGG + chr2.3:75692416-75692435 None:intergenic 45.0%
TGAGAAATCTCCATGCCAAG+CGG + chr2.3:75692574-75692593 None:intergenic 45.0%
TGCAATTGGGAACCTCATGA+GGG + chr2.3:75692444-75692463 None:intergenic 45.0%
TTGCAATTGGGAACCTCATG+AGG + chr2.3:75692445-75692464 None:intergenic 45.0%
! AGTAATGGAGGCAGTGAAGT+TGG - chr2.3:75692825-75692844 MS.gene002063:CDS 45.0%
! GCATATTCTAAGTGGCCTTG+TGG + chr2.3:75692244-75692263 None:intergenic 45.0%
! TGAGTTGGAGAAGGCAGTAA+TGG - chr2.3:75692810-75692829 MS.gene002063:CDS 45.0%
!! ATGTAAGCATTGGAGGAGGA+AGG + chr2.3:75692271-75692290 None:intergenic 45.0%
!! GGAGTGAATGTAAGCATTGG+AGG + chr2.3:75692278-75692297 None:intergenic 45.0%
!! GTGAATGTAAGCATTGGAGG+AGG + chr2.3:75692275-75692294 None:intergenic 45.0%
AAATCTCCATGCCAAGCGGA+GGG + chr2.3:75692570-75692589 None:intergenic 50.0%
AAGTTTCACCATGCCTGCCA+CGG - chr2.3:75692631-75692650 MS.gene002063:CDS 50.0%
ATGTCTGCCAGATTTGAGGG+TGG + chr2.3:75692168-75692187 None:intergenic 50.0%
ATTTGAGGGTGGACATGCGA+TGG + chr2.3:75692157-75692176 None:intergenic 50.0%
CTGACGTTCATGGTGAAGTC+TGG - chr2.3:75692608-75692627 MS.gene002063:CDS 50.0%
GACCTCATCAGTGTGAGGAT+CGG + chr2.3:75692365-75692384 None:intergenic 50.0%
GTTGGAGAAGGCAGTAATGG+AGG - chr2.3:75692813-75692832 MS.gene002063:CDS 50.0%
TCATGCCAATCATCACCTCG+AGG + chr2.3:75692886-75692905 None:intergenic 50.0%
TGGTATTTGGACAGCAGTGC+CGG + chr2.3:75692194-75692213 None:intergenic 50.0%
! ATCTTTCTTTTGCTCCGCCG+CGG + chr2.3:75692791-75692810 None:intergenic 50.0%
AGTTTCACCATGCCTGCCAC+GGG - chr2.3:75692632-75692651 MS.gene002063:CDS 55.0%
ATGGTTGCTGCTGTTGGTGC+TGG + chr2.3:75692138-75692157 None:intergenic 55.0%
CACCATGAACGTCAGTGACG+AGG + chr2.3:75692603-75692622 None:intergenic 55.0%
CATGAACGTCAGTGACGAGG+AGG + chr2.3:75692600-75692619 None:intergenic 55.0%
CATGCGATGGTTGCTGCTGT+TGG + chr2.3:75692144-75692163 None:intergenic 55.0%
CGCATGTCCACCCTCAAATC+TGG - chr2.3:75692158-75692177 MS.gene002063:intron 55.0%
CGTTTCTCTTGGCAAACCCG+TGG + chr2.3:75692651-75692670 None:intergenic 55.0%
CTCATGAGGGTCTTGGACAC+AGG + chr2.3:75692431-75692450 None:intergenic 55.0%
CTCCTCGTCACTGACGTTCA+TGG - chr2.3:75692598-75692617 MS.gene002063:CDS 55.0%
CTGTGTCCAAGACCCTCATG+AGG - chr2.3:75692429-75692448 MS.gene002063:CDS 55.0%
GAAATCTCCATGCCAAGCGG+AGG + chr2.3:75692571-75692590 None:intergenic 55.0%
GCGAAGACCTCATCAGTGTG+AGG + chr2.3:75692370-75692389 None:intergenic 55.0%
TATCTTCCCTCCGCTTGGCA+TGG - chr2.3:75692561-75692580 MS.gene002063:CDS 55.0%
TGGGAACCTCATGAGGGTCT+TGG + chr2.3:75692438-75692457 None:intergenic 55.0%
ATCGGCGAGGAGATCAACCG+CGG + chr2.3:75692347-75692366 None:intergenic 60.0%
CATCAGTGTGAGGATCGGCG+AGG + chr2.3:75692360-75692379 None:intergenic 60.0%
CGCCGATCCTCACACTGATG+AGG - chr2.3:75692360-75692379 MS.gene002063:CDS 60.0%
GGAGGCAGTGAAGTTGGCAG+AGG - chr2.3:75692831-75692850 MS.gene002063:CDS 60.0%
TCTCTTGGCAAACCCGTGGC+AGG + chr2.3:75692647-75692666 None:intergenic 60.0%
TGGGATTCGCAGGAAAGCCG+CGG - chr2.3:75692771-75692790 MS.gene002063:CDS 60.0%
GATTCGCAGGAAAGCCGCGG+CGG - chr2.3:75692774-75692793 MS.gene002063:CDS 65.0%
TGGCAAACCCGTGGCAGGCA+TGG + chr2.3:75692642-75692661 None:intergenic 65.0%
!! GCTGCTGTTGGTGCTGGTGC+TGG + chr2.3:75692132-75692151 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 75692118 75693192 75692118 ID=MS.gene002063
chr2.3 mRNA 75692118 75693192 75692118 ID=MS.gene002063.t1;Parent=MS.gene002063
chr2.3 exon 75692248 75693192 75692248 ID=MS.gene002063.t1.exon1;Parent=MS.gene002063.t1
chr2.3 CDS 75692248 75693192 75692248 ID=cds.MS.gene002063.t1;Parent=MS.gene002063.t1
chr2.3 exon 75692118 75692168 75692118 ID=MS.gene002063.t1.exon2;Parent=MS.gene002063.t1
chr2.3 CDS 75692118 75692168 75692118 ID=cds.MS.gene002063.t1;Parent=MS.gene002063.t1
Gene Sequence

>MS.gene002063

ATGTCTCTCAACCAGCACCAGCACCAACAGCAGCAACCATCGCATGTCCACCCTCAAATCTGGCAGACATTAACCGGCACTGCTGTCCAAATACCAAAGTTGCATCCAAGAGTTTACTATTTTCCACAAGGCCACTTAGAATATGCTTCTCCTTCCTCCTCCAATGCTTACATTCACTCCCTTCATGATCTTCAACGTTTTCGTCCTTTCACTATCTGTATCATCTCCGCGGTTGATCTCCTCGCCGATCCTCACACTGATGAGGTCTTCGCTAAACTGCTACTCACTCCAATAACCAATGATGTTGATGCCTGTGTCCAAGACCCTCATGAGGTTCCCAATTGCAACGACGATGATGAGGTAATTGGTTCGTTTACAAGGACTCTCGCCCCTACTAATGTAAGCAAGCATACATTTTACATACCTCGGTTTTGTGCTGAAAATATCTTCCCTCCGCTTGGCATGGAGATTTCTCAAAACCTCCTCGTCACTGACGTTCATGGTGAAGTCTGGAAGTTTCACCATGCCTGCCACGGGTTTGCCAAGAGAAACGTGTTTTATATCCCTGAATGGAGTTCGTTTGTTAAGAGGAAGAAACTCGACGTCGGAGATGCTGTTGTTTTCATGAAAAACTCAACGGGAAAATTATTTGTTGGGATTCGCAGGAAAGCCGCGGCGGAGCAAAAGAAAGATGAGTTGGAGAAGGCAGTAATGGAGGCAGTGAAGTTGGCAGAGGAGAACAAGCCATTTGAAATTGTTTACTATCCTCGAGGTGATGATTGGCATGATTTTGTAGTGGATGCTAATATTGTTGATGAATCAATGAAAATTCAATGGAACCCTAGAATGAGAGTTAAGATGAAGACTCATAAATCTTCAAGGATCCCATATCAAGGGACAATCTCTACTGTATCTCGTACGTCTAATTTATGGCGCATGCTACAGGTATAATTTCTTCTTTTTTTTTTTTGGATAGTATTTAGTTAAAGTTGATAAATGATTCAAATGCTGAATCCATAATTAGATGACACTTCATAAGTACCCGTTTATTTTGCTTCACAATGAAAATACCTAA
Protein sequence

>MS.gene002063.t1

MSLNQHQHQQQQPSHVHPQIWQTLTGTAVQIPKLHPRVYYFPQGHLEYASPSSSNAYIHSLHDLQRFRPFTICIISAVDLLADPHTDEVFAKLLLTPITNDVDACVQDPHEVPNCNDDDEVIGSFTRTLAPTNVSKHTFYIPRFCAENIFPPLGMEISQNLLVTDVHGEVWKFHHACHGFAKRNVFYIPEWSSFVKRKKLDVGDAVVFMKNSTGKLFVGIRRKAAAEQKKDELEKAVMEAVKLAEENKPFEIVYYPRGDDWHDFVVDANIVDESMKIQWNPRMRVKMKTHKSSRIPYQGTISTVSRTSNLWRMLQMTLHKYPFILLHNENT