AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002145

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002145.t1 MTR_2g007930 99.766 427 1 0 1 427 1 427 0.0 886
MS.gene002145.t1 MTR_1g098790 80.230 435 74 5 1 427 1 431 0.0 692
MS.gene002145.t1 MTR_5g020580 55.300 434 169 12 1 427 1 416 1.04e-155 446
MS.gene002145.t1 MTR_2g020280 58.005 381 137 6 49 427 34 393 1.36e-154 443
MS.gene002145.t1 MTR_1g088960 53.241 432 167 10 1 427 1 402 7.50e-154 441
MS.gene002145.t1 MTR_8g077195 52.791 430 182 8 1 427 1 412 8.72e-149 429
MS.gene002145.t1 MTR_1g022370 49.777 448 178 9 1 427 1 422 1.60e-141 410
MS.gene002145.t1 MTR_2g020280 57.143 315 112 6 115 427 1 294 2.96e-119 349
MS.gene002145.t1 MTR_2g076200 48.670 376 145 9 54 427 42 371 1.50e-116 345
MS.gene002145.t1 MTR_8g077620 40.421 428 205 14 1 420 1 386 9.20e-93 285
MS.gene002145.t1 MTR_2g076210 42.632 380 163 11 54 427 37 367 1.31e-92 284
MS.gene002145.t1 MTR_8g077675 44.389 401 178 14 32 420 28 395 2.34e-91 282
MS.gene002145.t1 MTR_8g077650 40.263 380 153 15 49 419 14 328 6.79e-67 216
MS.gene002145.t1 MTR_1g023800 51.656 151 65 4 1 148 1 146 1.14e-43 150
MS.gene002145.t1 MTR_8g077640 43.172 227 97 6 1 207 46 260 2.56e-41 150
MS.gene002145.t1 MTR_8g077640 45.455 99 47 2 320 417 291 383 1.98e-16 80.9
MS.gene002145.t1 MTR_0210s0010 40.404 99 52 2 96 194 8 99 5.47e-15 71.2
MS.gene002145.t1 MTR_2g105390 40.909 110 44 5 323 426 265 359 4.89e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002145.t1 AT1G25280 73.392 451 90 14 1 427 1 445 0.0 612
MS.gene002145.t1 AT1G76900 70.130 462 96 11 1 427 1 455 0.0 609
MS.gene002145.t1 AT1G76900 70.130 462 96 11 1 427 1 455 0.0 609
MS.gene002145.t1 AT1G76900 70.130 462 96 11 1 427 1 455 0.0 609
MS.gene002145.t1 AT1G43640 68.552 442 111 7 1 427 1 429 0.0 605
MS.gene002145.t1 AT1G43640 70.400 375 86 5 66 427 1 363 0.0 535
MS.gene002145.t1 AT2G47900 55.093 432 163 7 1 427 1 406 3.63e-167 475
MS.gene002145.t1 AT2G47900 55.093 432 163 7 1 427 1 406 3.63e-167 475
MS.gene002145.t1 AT2G47900 54.965 433 163 8 1 427 1 407 1.09e-165 472
MS.gene002145.t1 AT2G18280 52.995 434 156 9 1 427 1 393 1.37e-146 422
MS.gene002145.t1 AT2G18280 52.995 434 156 9 1 427 1 393 1.37e-146 422
MS.gene002145.t1 AT1G47270 50.230 434 163 8 1 427 1 388 8.90e-140 405
MS.gene002145.t1 AT1G47270 50.230 434 163 8 1 427 26 413 1.19e-139 405
MS.gene002145.t1 AT1G47270 50.230 434 163 8 1 427 25 412 1.23e-139 405
MS.gene002145.t1 AT3G06380 51.979 379 146 8 54 427 33 380 3.41e-128 375
MS.gene002145.t1 AT5G18680 49.148 411 162 11 32 427 11 389 1.71e-125 369
MS.gene002145.t1 AT1G25280 73.063 271 52 11 174 427 1 267 8.60e-124 360
MS.gene002145.t1 AT1G25280 73.063 271 52 11 174 427 1 267 8.60e-124 360
MS.gene002145.t1 AT1G53320 48.294 381 151 8 51 427 41 379 1.02e-115 343
MS.gene002145.t1 AT5G18680 51.703 323 116 9 113 427 7 297 7.53e-101 302
MS.gene002145.t1 AT3G06380 51.111 315 119 7 117 427 5 288 6.85e-100 300
MS.gene002145.t1 AT1G61940 56.977 86 37 0 57 142 1 86 8.72e-31 119
MS.gene002145.t1 AT1G61940 60.656 61 24 0 57 117 1 61 3.13e-19 87.0

Find 118 sgRNAs with CRISPR-Local

Find 180 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAGCTTTGTTAGTTGAAAA+TGG 0.215408 2.2:+72137451 MS.gene002145:intron
ATCTGCAACTAACCTGTTTC+AGG 0.217420 2.2:-72136728 None:intergenic
TACACCAGATCAAACTTTCT+TGG 0.237446 2.2:+72138041 MS.gene002145:intron
ATGACATCAAATAGTAATTC+TGG 0.251135 2.2:-72136561 None:intergenic
CGTGACGTTCGTGATAGTTT+TGG 0.276663 2.2:+72136408 MS.gene002145:CDS
CCCGCATCAAGTGCTGAATT+TGG 0.304909 2.2:-72138272 None:intergenic
CAGTCCCCGGAGGACATATA+TGG 0.310396 2.2:-72138109 None:intergenic
GTGCTTCTGTTTGCCAGTCT+TGG 0.312781 2.2:+72136640 MS.gene002145:CDS
GATGCGGGCGGCTCCGTTCC+CGG 0.316477 2.2:+72138287 MS.gene002145:CDS
CAGAATAATCTTATCATGTT+CGG 0.319403 2.2:-72138621 None:intergenic
GCTTCCACCCAACCACCTGC+TGG 0.321946 2.2:+72138569 MS.gene002145:CDS
GCCGTCATCATCCTCATTAC+AGG 0.338459 2.2:-72138426 None:intergenic
TTGTAATTCAAAATAGTTGC+TGG 0.356421 2.2:+72136526 MS.gene002145:CDS
GTGAATTAGCTCTGGCTGTC+CGG 0.359818 2.2:-72138306 None:intergenic
TCATTCAATGCTTTATCAAA+AGG 0.375284 2.2:+72136873 MS.gene002145:CDS
CATATATGTCCTCCGGGGAC+TGG 0.388613 2.2:+72138110 MS.gene002145:CDS
ACATATATGGGCAGAAGTGC+AGG 0.394714 2.2:-72138096 None:intergenic
GTTAGTTTGCCGCAGAACTC+TGG 0.395072 2.2:-72136693 None:intergenic
TTCTGCAATTCGGCAAAGTT+GGG 0.396243 2.2:+72138639 MS.gene002145:CDS
AATTACTATTTGATGTCATT+CGG 0.397064 2.2:+72136565 MS.gene002145:CDS
AAAGCTTGGAATGCAGATAA+AGG 0.399095 2.2:-72138692 None:intergenic
TTACTCGTCCCCGAAAATTA+AGG 0.402869 2.2:-72138514 None:intergenic
ATTCCCTGTGTCCCTGAAAC+AGG 0.406088 2.2:+72136716 MS.gene002145:CDS
TTCTGCCCATATATGTCCTC+CGG 0.408726 2.2:+72138103 MS.gene002145:CDS
CTTCCACCCAACCACCTGCT+GGG 0.409907 2.2:+72138570 MS.gene002145:CDS
CACTGAGTATGTTATTTCAA+TGG 0.410829 2.2:+72137516 MS.gene002145:CDS
GTGAGAATACTTGGCCTTCA+AGG 0.416059 2.2:+72136601 MS.gene002145:CDS
TCGGCGCCCCAGCAGGTGGT+TGG 0.419959 2.2:-72138577 None:intergenic
CTTCTGTTTGCCAGTCTTGG+AGG 0.420970 2.2:+72136643 MS.gene002145:CDS
TGTAATTGTTCATGCCATCT+TGG 0.429018 2.2:-72138482 None:intergenic
CTCAGTGTAGGTTGTTCTCC+TGG 0.432833 2.2:-72137500 None:intergenic
TAACTCCCAGATGGGACCTT+TGG 0.438868 2.2:-72138167 None:intergenic
GCAACTACATGCTTCCTTGA+AGG 0.442687 2.2:-72136615 None:intergenic
TGCTCCGGAACGAGTCCTCC+AGG 0.444143 2.2:-72138334 None:intergenic
GTATCGCCAAAGGTCCCATC+TGG 0.445185 2.2:+72138161 MS.gene002145:CDS
GATGGTGTCGGCGCCCCAGC+AGG 0.447033 2.2:-72138584 None:intergenic
GATAAGATTATTCTGCAATT+CGG 0.448717 2.2:+72138629 MS.gene002145:CDS
CGGCGCCCCAGCAGGTGGTT+GGG 0.449066 2.2:-72138576 None:intergenic
AGAATAATCTTATCATGTTC+GGG 0.450318 2.2:-72138620 None:intergenic
ATTCTGCAATTCGGCAAAGT+TGG 0.451251 2.2:+72138638 MS.gene002145:CDS
ATATATGTCCTCCGGGGACT+GGG 0.453188 2.2:+72138111 MS.gene002145:CDS
TGACATCAAATAGTAATTCT+GGG 0.457179 2.2:-72136560 None:intergenic
TAATGCAATGCATCTTCCTT+GGG 0.462060 2.2:-72138238 None:intergenic
CATTCAATGCTTTATCAAAA+GGG 0.462254 2.2:+72136874 MS.gene002145:CDS
CAGGGCTCAGACAAAGGAAT+AGG 0.465192 2.2:-72136919 None:intergenic
AATTGTTCATGCCATCTTGG+AGG 0.466985 2.2:-72138479 None:intergenic
TGACGGCAAGATGAGACCTT+TGG 0.479423 2.2:+72138442 MS.gene002145:CDS
TCTGCCCATATATGTCCTCC+GGG 0.481297 2.2:+72138104 MS.gene002145:CDS
TGAATTAGCTCTGGCTGTCC+GGG 0.484556 2.2:-72138305 None:intergenic
TAGAGGAGAGTGAGAATACT+TGG 0.484573 2.2:+72136592 MS.gene002145:CDS
CTATTCCTTTGTCTGAGCCC+TGG 0.486417 2.2:+72136920 MS.gene002145:CDS
ATAATGCAATGCATCTTCCT+TGG 0.491165 2.2:-72138239 None:intergenic
AAACTATTCACAAGCCAACT+TGG 0.491824 2.2:-72138741 None:intergenic
TTATCATGTTCGGGTGGTGC+TGG 0.495403 2.2:-72138611 None:intergenic
TTAAGCATATTGCAAAAGCT+TGG 0.496726 2.2:-72138706 None:intergenic
GTTCCCTGGAGGACTCGTTC+CGG 0.496788 2.2:+72138330 MS.gene002145:CDS
ATGATTCCGTCTCGTGGCCC+TGG 0.498403 2.2:-72136854 None:intergenic
TCCTCTCTGCTAAGAGGACC+AGG 0.507703 2.2:+72137482 MS.gene002145:CDS
TTAGTTTGCCGCAGAACTCT+GGG 0.509843 2.2:-72136692 None:intergenic
AAAGTCTCATGGTTCTGTAC+AGG 0.510601 2.2:+72136485 MS.gene002145:CDS
TGTAATTCAAAATAGTTGCT+GGG 0.513418 2.2:+72136527 MS.gene002145:CDS
AGAGCTAATTCACCGTTCCC+TGG 0.515311 2.2:+72138316 MS.gene002145:CDS
ACCAAATTCAGCACTTGATG+CGG 0.517901 2.2:+72138271 MS.gene002145:CDS
ACTTCGAAAATGAGATGCTC+CGG 0.519823 2.2:-72138349 None:intergenic
CGCCCCAGCAGGTGGTTGGG+TGG 0.525317 2.2:-72138573 None:intergenic
AGGGAACGGTGAATTAGCTC+TGG 0.526856 2.2:-72138314 None:intergenic
TAGCTCTGGCTGTCCGGGAA+CGG 0.532391 2.2:-72138300 None:intergenic
GAGACTTTCCTCTATGATGA+TGG 0.534079 2.2:-72136470 None:intergenic
TGCACATATTCCTCCAAGAC+TGG 0.534318 2.2:-72136653 None:intergenic
AATTCCTCCTCTCTGCTAAG+AGG 0.537777 2.2:+72137476 MS.gene002145:CDS
GTTTACCAGGGCTCAGACAA+AGG 0.542225 2.2:-72136925 None:intergenic
TTCCACCCAACCACCTGCTG+GGG 0.546085 2.2:+72138571 MS.gene002145:CDS
GGTGCTGGTTGAGATGGTGT+CGG 0.550204 2.2:-72138596 None:intergenic
CCAAATTCAGCACTTGATGC+GGG 0.550546 2.2:+72138272 MS.gene002145:CDS
GGTGTCGGCGCCCCAGCAGG+TGG 0.552680 2.2:-72138581 None:intergenic
CAATCGAGTTCAGCAGTGCA+CGG 0.554602 2.2:+72138384 MS.gene002145:CDS
AAGCAGCTTTGACACCAAGT+TGG 0.560190 2.2:+72138727 MS.gene002145:CDS
CGGCTCGTCTTCCCAGTCCC+CGG 0.560397 2.2:-72138122 None:intergenic
TCGGGTGGTGCTGGTTGAGA+TGG 0.560858 2.2:-72138602 None:intergenic
AGTCCCCGGAGGACATATAT+GGG 0.561123 2.2:-72138108 None:intergenic
AGATCAAGTAATACTTACAT+TGG 0.570296 2.2:+72137556 MS.gene002145:CDS
ACATATGAACTAAATGTGCT+CGG 0.570299 2.2:+72138206 MS.gene002145:CDS
CTAAATGTGCTCGGAACTCG+AGG 0.572448 2.2:+72138215 MS.gene002145:CDS
CTAACCTGTTTCAGGGACAC+AGG 0.573911 2.2:-72136720 None:intergenic
TCTGCAACTAACCTGTTTCA+GGG 0.575798 2.2:-72136727 None:intergenic
TGGGAGTTACAACATAGCTC+AGG 0.584581 2.2:+72138181 MS.gene002145:CDS
CTTACTGGCCATCATCATAG+AGG 0.586395 2.2:+72136462 MS.gene002145:CDS
GACATCAAATAGTAATTCTG+GGG 0.586583 2.2:-72136559 None:intergenic
GGCATGAACAATTACAGTGC+TGG 0.588906 2.2:+72138489 MS.gene002145:CDS
TGATGTCATTCGGCGACTAG+AGG 0.590244 2.2:+72136575 MS.gene002145:CDS
GGAACGAGTCCTCCAGGGAA+CGG 0.592037 2.2:-72138328 None:intergenic
CATCATAGAGGAAAGTCTCA+TGG 0.596493 2.2:+72136474 MS.gene002145:CDS
TGAAATAACATACTCAGTGT+AGG 0.597863 2.2:-72137512 None:intergenic
TAAAGGATATCGATAATCCA+TGG 0.599763 2.2:-72138675 None:intergenic
GCTATGTTGTAACTCCCAGA+TGG 0.603839 2.2:-72138176 None:intergenic
CATTGAATGATTCCGTCTCG+TGG 0.607917 2.2:-72136860 None:intergenic
CTATGTTGTAACTCCCAGAT+GGG 0.609999 2.2:-72138175 None:intergenic
TGCTCGGAACTCGAGGCCCA+AGG 0.612968 2.2:+72138222 MS.gene002145:CDS
TATCGCCAAAGGTCCCATCT+GGG 0.614613 2.2:+72138162 MS.gene002145:CDS
TTTCAGCCAGGGCCACGAGA+CGG 0.619741 2.2:+72136848 MS.gene002145:intron
GAACGTCACGAACTATGCTA+CGG 0.624312 2.2:-72136395 None:intergenic
TAACCTGTTTCAGGGACACA+GGG 0.626828 2.2:-72136719 None:intergenic
TCCTGGTCCTCTTAGCAGAG+AGG 0.627316 2.2:-72137483 None:intergenic
CATATATGGGCAGAAGTGCA+GGG 0.629040 2.2:-72138095 None:intergenic
TCCTGTAATGAGGATGATGA+CGG 0.629322 2.2:+72138425 MS.gene002145:CDS
GTCTTGGAGGAATATGTGCA+AGG 0.636885 2.2:+72136656 MS.gene002145:CDS
TGGGAAAGATATGTTCACCA+TGG 0.642687 2.2:+72138658 MS.gene002145:CDS
GTTAAGAGCCCAGAGTTCTG+CGG 0.649777 2.2:+72136684 MS.gene002145:CDS
TGGTCCTCTTAGCAGAGAGG+AGG 0.654986 2.2:-72137480 None:intergenic
GCTCCGGAACGAGTCCTCCA+GGG 0.659722 2.2:-72138333 None:intergenic
ATACTTACATTGGAAAACTG+AGG 0.671640 2.2:+72137566 MS.gene002145:CDS
ACATCAAATAGTAATTCTGG+GGG 0.673779 2.2:-72136558 None:intergenic
AATTCAGCACTTGATGCGGG+CGG 0.674604 2.2:+72138275 MS.gene002145:CDS
ATAATCTTATCATGTTCGGG+TGG 0.690693 2.2:-72138617 None:intergenic
CTCGTCTTCCCAGTCCCCGG+AGG 0.692752 2.2:-72138119 None:intergenic
GCTAATTCACCGTTCCCTGG+AGG 0.694493 2.2:+72138319 MS.gene002145:CDS
CTGCCCATATATGTCCTCCG+GGG 0.741928 2.2:+72138105 MS.gene002145:CDS
ATATGGGCAGAAGTGCAGGG+AGG 0.788147 2.2:-72138092 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGTAAATAAAATTGAAGC+AGG - chr2.2:72137424-72137443 None:intergenic 20.0%
!! AATTACTATTTGATGTCATT+CGG + chr2.2:72136565-72136584 MS.gene002145:CDS 20.0%
!! CATAAGTGGAAAATTATTTA+TGG - chr2.2:72137906-72137925 None:intergenic 20.0%
!! TGAAATAACAACAATAGTTA+AGG + chr2.2:72136792-72136811 MS.gene002145:intron 20.0%
!! TTTCAAAATTTAAGCAGTTT+AGG + chr2.2:72137041-72137060 MS.gene002145:intron 20.0%
!!! ATACCTTTTTAGAAAATCTA+CGG - chr2.2:72138145-72138164 None:intergenic 20.0%
!!! CTATGTTAAAAACTTCTTTT+CGG + chr2.2:72137629-72137648 MS.gene002145:intron 20.0%
! AAATATTCATGGAATGACAA+CGG - chr2.2:72137221-72137240 None:intergenic 25.0%
! AGAATAATCTTATCATGTTC+GGG - chr2.2:72138623-72138642 None:intergenic 25.0%
! AGATCAAGTAATACTTACAT+TGG + chr2.2:72137556-72137575 MS.gene002145:CDS 25.0%
! ATGACATCAAATAGTAATTC+TGG - chr2.2:72136564-72136583 None:intergenic 25.0%
! CAAAGGGAAATAAATATTCA+TGG - chr2.2:72137232-72137251 None:intergenic 25.0%
! CAGAATAATCTTATCATGTT+CGG - chr2.2:72138624-72138643 None:intergenic 25.0%
! CATTCAATGCTTTATCAAAA+GGG + chr2.2:72136874-72136893 MS.gene002145:CDS 25.0%
! GATAAGATTATTCTGCAATT+CGG + chr2.2:72138629-72138648 MS.gene002145:CDS 25.0%
! TCATTCAATGCTTTATCAAA+AGG + chr2.2:72136873-72136892 MS.gene002145:CDS 25.0%
! TGACATCAAATAGTAATTCT+GGG - chr2.2:72136563-72136582 None:intergenic 25.0%
! TGTAATTCAAAATAGTTGCT+GGG + chr2.2:72136527-72136546 MS.gene002145:CDS 25.0%
! TGTGTTGTAACTGTTATTAT+CGG + chr2.2:72137851-72137870 MS.gene002145:intron 25.0%
! TTGTAATTCAAAATAGTTGC+TGG + chr2.2:72136526-72136545 MS.gene002145:CDS 25.0%
!! TCTTTTATCGTCATATGTTT+AGG + chr2.2:72137802-72137821 MS.gene002145:intron 25.0%
!! TGATTCTTCTGTTTTACTTT+TGG - chr2.2:72137732-72137751 None:intergenic 25.0%
!!! AACATCTCATTTTTATGCAT+CGG - chr2.2:72137191-72137210 None:intergenic 25.0%
!!! CAACTATTTTGAATTACAAG+AGG - chr2.2:72136525-72136544 None:intergenic 25.0%
!!! GATTCTTCTGTTTTACTTTT+GGG - chr2.2:72137731-72137750 None:intergenic 25.0%
!!! GTTTAATTTGAGATCTACTT+TGG + chr2.2:72137138-72137157 MS.gene002145:intron 25.0%
!!! TTAAAATGCGTTTTGATAGT+CGG - chr2.2:72137706-72137725 None:intergenic 25.0%
AACAGAAAAAACTCAGCAAA+TGG - chr2.2:72137969-72137988 None:intergenic 30.0%
AAGTTGCAATGAATTTCAGT+TGG + chr2.2:72137763-72137782 MS.gene002145:intron 30.0%
ACATATGAACTAAATGTGCT+CGG + chr2.2:72138206-72138225 MS.gene002145:CDS 30.0%
ACATCAAATAGTAATTCTGG+GGG - chr2.2:72136561-72136580 None:intergenic 30.0%
ATACTTACATTGGAAAACTG+AGG + chr2.2:72137566-72137585 MS.gene002145:CDS 30.0%
CTGTTTGATATCCATTATGT+CGG + chr2.2:72137279-72137298 MS.gene002145:intron 30.0%
GAAGATATGAATAGCAAACA+AGG - chr2.2:72137942-72137961 None:intergenic 30.0%
GACATCAAATAGTAATTCTG+GGG - chr2.2:72136562-72136581 None:intergenic 30.0%
TAAAACCATATATACCCACT+AGG - chr2.2:72136973-72136992 None:intergenic 30.0%
TAAAGGATATCGATAATCCA+TGG - chr2.2:72138678-72138697 None:intergenic 30.0%
TGAAATAACATACTCAGTGT+AGG - chr2.2:72137515-72137534 None:intergenic 30.0%
TTAACATAGTAGATGAGAGA+AGG - chr2.2:72137618-72137637 None:intergenic 30.0%
TTAAGCATATTGCAAAAGCT+TGG - chr2.2:72138709-72138728 None:intergenic 30.0%
!! AGTTTTGATGTCAAACTTAC+TGG + chr2.2:72136447-72136466 MS.gene002145:CDS 30.0%
!! CACTGAGTATGTTATTTCAA+TGG + chr2.2:72137516-72137535 MS.gene002145:CDS 30.0%
!!! ACTTCTAAAGTTTTGATGCT+AGG + chr2.2:72137358-72137377 MS.gene002145:intron 30.0%
!!! CTTCTAAAGTTTTGATGCTA+GGG + chr2.2:72137359-72137378 MS.gene002145:intron 30.0%
AAAGCTTGGAATGCAGATAA+AGG - chr2.2:72138695-72138714 None:intergenic 35.0%
ACTCCTAGAATCAATCACAA+AGG - chr2.2:72137249-72137268 None:intergenic 35.0%
ATAATCTTATCATGTTCGGG+TGG - chr2.2:72138620-72138639 None:intergenic 35.0%
ATAATGCAATGCATCTTCCT+TGG - chr2.2:72138242-72138261 None:intergenic 35.0%
CGGAGGTAATAAATAGTCTA+GGG + chr2.2:72137871-72137890 MS.gene002145:intron 35.0%
CTCCTAGAATCAATCACAAA+GGG - chr2.2:72137248-72137267 None:intergenic 35.0%
GAATATCAGCATCACATAAG+TGG - chr2.2:72137920-72137939 None:intergenic 35.0%
GTTGTAACTGTTATTATCGG+AGG + chr2.2:72137854-72137873 MS.gene002145:intron 35.0%
TAATGCAATGCATCTTCCTT+GGG - chr2.2:72138241-72138260 None:intergenic 35.0%
TACACCAGATCAAACTTTCT+TGG + chr2.2:72138041-72138060 MS.gene002145:intron 35.0%
TATATACCCACTAGGTAACT+AGG - chr2.2:72136965-72136984 None:intergenic 35.0%
TCGGAGGTAATAAATAGTCT+AGG + chr2.2:72137870-72137889 MS.gene002145:intron 35.0%
TGTAATTGTTCATGCCATCT+TGG - chr2.2:72138485-72138504 None:intergenic 35.0%
TGTTCCAAGAAAGTTTGATC+TGG - chr2.2:72138048-72138067 None:intergenic 35.0%
TTGATATCCATTATGTCGGA+GGG + chr2.2:72137283-72137302 MS.gene002145:intron 35.0%
TTTGATATCCATTATGTCGG+AGG + chr2.2:72137282-72137301 MS.gene002145:intron 35.0%
! AGTTACCTAGTGGGTATATA+TGG + chr2.2:72136965-72136984 MS.gene002145:intron 35.0%
! GTTTTATGTTCAGATGCTTC+TGG + chr2.2:72136987-72137006 MS.gene002145:intron 35.0%
! TTCCCTTTGTGATTGATTCT+AGG + chr2.2:72137243-72137262 MS.gene002145:intron 35.0%
!! GAGCCGTAGATTTTCTAAAA+AGG + chr2.2:72138139-72138158 MS.gene002145:CDS 35.0%
!! GCAGCTTTGTTAGTTGAAAA+TGG + chr2.2:72137451-72137470 MS.gene002145:intron 35.0%
!! TTCTAAAAAGGTATCGCCAA+AGG + chr2.2:72138151-72138170 MS.gene002145:CDS 35.0%
!!! ATAGTTTTGGGAGCTTATCT+AGG + chr2.2:72136421-72136440 MS.gene002145:CDS 35.0%
AATTGTTCATGCCATCTTGG+AGG - chr2.2:72138482-72138501 None:intergenic 40.0%
ACCAAATTCAGCACTTGATG+CGG + chr2.2:72138271-72138290 MS.gene002145:CDS 40.0%
ACTTCGAAAATGAGATGCTC+CGG - chr2.2:72138352-72138371 None:intergenic 40.0%
ATCTGCAACTAACCTGTTTC+AGG - chr2.2:72136731-72136750 None:intergenic 40.0%
ATTCTGCAATTCGGCAAAGT+TGG + chr2.2:72138638-72138657 MS.gene002145:CDS 40.0%
CATCATAGAGGAAAGTCTCA+TGG + chr2.2:72136474-72136493 MS.gene002145:CDS 40.0%
CTATGTTGTAACTCCCAGAT+GGG - chr2.2:72138178-72138197 None:intergenic 40.0%
GAGACTTTCCTCTATGATGA+TGG - chr2.2:72136473-72136492 None:intergenic 40.0%
TAGAGGAGAGTGAGAATACT+TGG + chr2.2:72136592-72136611 MS.gene002145:CDS 40.0%
TCCTGTAATGAGGATGATGA+CGG + chr2.2:72138425-72138444 MS.gene002145:CDS 40.0%
TCTGCAACTAACCTGTTTCA+GGG - chr2.2:72136730-72136749 None:intergenic 40.0%
TGAAAAACAAGCCTCCAAGA+TGG + chr2.2:72138468-72138487 MS.gene002145:CDS 40.0%
TGGGAAAGATATGTTCACCA+TGG + chr2.2:72138658-72138677 MS.gene002145:CDS 40.0%
TTACTCGTCCCCGAAAATTA+AGG - chr2.2:72138517-72138536 None:intergenic 40.0%
TTCTGCAATTCGGCAAAGTT+GGG + chr2.2:72138639-72138658 MS.gene002145:CDS 40.0%
! AAAGTCTCATGGTTCTGTAC+AGG + chr2.2:72136485-72136504 MS.gene002145:CDS 40.0%
! GTGACGTTCGTGATAGTTTT+GGG + chr2.2:72136409-72136428 MS.gene002145:CDS 40.0%
! GTGATTGATTCTAGGAGTCT+TGG + chr2.2:72137251-72137270 MS.gene002145:intron 40.0%
!! CGTTTTGATAGTCGGAAACT+CGG - chr2.2:72137698-72137717 None:intergenic 40.0%
!! GGCTTGTTTTTCAGAACCAA+AGG - chr2.2:72138461-72138480 None:intergenic 40.0%
!! TAAGCGAAGAGTGTTTACCA+GGG - chr2.2:72136940-72136959 None:intergenic 40.0%
!! TTCGTTTCACTTTTCAGCCA+GGG + chr2.2:72136837-72136856 MS.gene002145:intron 40.0%
!!! AGTGCTGGTGCCTTAATTTT+CGG + chr2.2:72138504-72138523 MS.gene002145:CDS 40.0%
AATTCCTCCTCTCTGCTAAG+AGG + chr2.2:72137476-72137495 MS.gene002145:CDS 45.0%
CATTGAATGATTCCGTCTCG+TGG - chr2.2:72136863-72136882 None:intergenic 45.0%
CCAAATTCAGCACTTGATGC+GGG + chr2.2:72138272-72138291 MS.gene002145:CDS 45.0%
CTTACGCCTAGTTACCTAGT+GGG + chr2.2:72136956-72136975 MS.gene002145:intron 45.0%
CTTACTGGCCATCATCATAG+AGG + chr2.2:72136462-72136481 MS.gene002145:CDS 45.0%
GAACGTCACGAACTATGCTA+CGG - chr2.2:72136398-72136417 None:intergenic 45.0%
GCAACTACATGCTTCCTTGA+AGG - chr2.2:72136618-72136637 None:intergenic 45.0%
GCTATGTTGTAACTCCCAGA+TGG - chr2.2:72138179-72138198 None:intergenic 45.0%
GGCATGAACAATTACAGTGC+TGG + chr2.2:72138489-72138508 MS.gene002145:CDS 45.0%
GTCTTGGAGGAATATGTGCA+AGG + chr2.2:72136656-72136675 MS.gene002145:CDS 45.0%
TAACCTGTTTCAGGGACACA+GGG - chr2.2:72136722-72136741 None:intergenic 45.0%
TGCACATATTCCTCCAAGAC+TGG - chr2.2:72136656-72136675 None:intergenic 45.0%
TGGGAGTTACAACATAGCTC+AGG + chr2.2:72138181-72138200 MS.gene002145:CDS 45.0%
TTCTGCCCATATATGTCCTC+CGG + chr2.2:72138103-72138122 MS.gene002145:CDS 45.0%
! AAGCAGCTTTGACACCAAGT+TGG + chr2.2:72138727-72138746 MS.gene002145:CDS 45.0%
! ACATATATGGGCAGAAGTGC+AGG - chr2.2:72138099-72138118 None:intergenic 45.0%
! CATATATGGGCAGAAGTGCA+GGG - chr2.2:72138098-72138117 None:intergenic 45.0%
! CGTGACGTTCGTGATAGTTT+TGG + chr2.2:72136408-72136427 MS.gene002145:CDS 45.0%
! GTGAGAATACTTGGCCTTCA+AGG + chr2.2:72136601-72136620 MS.gene002145:CDS 45.0%
! TGCTGGTGCCTTAATTTTCG+GGG + chr2.2:72138506-72138525 MS.gene002145:CDS 45.0%
!! GTAAGCGAAGAGTGTTTACC+AGG - chr2.2:72136941-72136960 None:intergenic 45.0%
!! GTGCTGGTGCCTTAATTTTC+GGG + chr2.2:72138505-72138524 MS.gene002145:CDS 45.0%
!! GTTCGTTTCACTTTTCAGCC+AGG + chr2.2:72136836-72136855 MS.gene002145:intron 45.0%
!! TTAGTTTGCCGCAGAACTCT+GGG - chr2.2:72136695-72136714 None:intergenic 45.0%
!!! AGTGCACGGTTTTCTGAGAT+CGG + chr2.2:72138398-72138417 MS.gene002145:CDS 45.0%
AATTCAGCACTTGATGCGGG+CGG + chr2.2:72138275-72138294 MS.gene002145:CDS 50.0%
AGAGCTAATTCACCGTTCCC+TGG + chr2.2:72138316-72138335 MS.gene002145:CDS 50.0%
AGGGAACGGTGAATTAGCTC+TGG - chr2.2:72138317-72138336 None:intergenic 50.0%
AGTCCCCGGAGGACATATAT+GGG - chr2.2:72138111-72138130 None:intergenic 50.0%
ATATATGTCCTCCGGGGACT+GGG + chr2.2:72138111-72138130 MS.gene002145:CDS 50.0%
ATCCATTATGTCGGAGGGCA+AGG + chr2.2:72137288-72137307 MS.gene002145:intron 50.0%
ATTCCCTGTGTCCCTGAAAC+AGG + chr2.2:72136716-72136735 MS.gene002145:CDS 50.0%
CAATCGAGTTCAGCAGTGCA+CGG + chr2.2:72138384-72138403 MS.gene002145:CDS 50.0%
CAGGGCTCAGACAAAGGAAT+AGG - chr2.2:72136922-72136941 None:intergenic 50.0%
CTAAATGTGCTCGGAACTCG+AGG + chr2.2:72138215-72138234 MS.gene002145:CDS 50.0%
CTAACCTGTTTCAGGGACAC+AGG - chr2.2:72136723-72136742 None:intergenic 50.0%
CTATTCCTTTGTCTGAGCCC+TGG + chr2.2:72136920-72136939 MS.gene002145:CDS 50.0%
CTCAGTGTAGGTTGTTCTCC+TGG - chr2.2:72137503-72137522 None:intergenic 50.0%
CTTCTGTTTGCCAGTCTTGG+AGG + chr2.2:72136643-72136662 MS.gene002145:CDS 50.0%
GCCGTCATCATCCTCATTAC+AGG - chr2.2:72138429-72138448 None:intergenic 50.0%
GCTTACGCCTAGTTACCTAG+TGG + chr2.2:72136955-72136974 MS.gene002145:intron 50.0%
GTGCTTCTGTTTGCCAGTCT+TGG + chr2.2:72136640-72136659 MS.gene002145:CDS 50.0%
GTTAAGAGCCCAGAGTTCTG+CGG + chr2.2:72136684-72136703 MS.gene002145:CDS 50.0%
GTTTACCAGGGCTCAGACAA+AGG - chr2.2:72136928-72136947 None:intergenic 50.0%
TAACTCCCAGATGGGACCTT+TGG - chr2.2:72138170-72138189 None:intergenic 50.0%
TATCGCCAAAGGTCCCATCT+GGG + chr2.2:72138162-72138181 MS.gene002145:CDS 50.0%
TCTGCCCATATATGTCCTCC+GGG + chr2.2:72138104-72138123 MS.gene002145:CDS 50.0%
TGACGGCAAGATGAGACCTT+TGG + chr2.2:72138442-72138461 MS.gene002145:CDS 50.0%
TGATGTCATTCGGCGACTAG+AGG + chr2.2:72136575-72136594 MS.gene002145:CDS 50.0%
TTATCATGTTCGGGTGGTGC+TGG - chr2.2:72138614-72138633 None:intergenic 50.0%
!! CCCGCATCAAGTGCTGAATT+TGG - chr2.2:72138275-72138294 None:intergenic 50.0%
!! GTGAATTAGCTCTGGCTGTC+CGG - chr2.2:72138309-72138328 None:intergenic 50.0%
!! GTTAGTTTGCCGCAGAACTC+TGG - chr2.2:72136696-72136715 None:intergenic 50.0%
!! TGAATTAGCTCTGGCTGTCC+GGG - chr2.2:72138308-72138327 None:intergenic 50.0%
!!! GTGCACGGTTTTCTGAGATC+GGG + chr2.2:72138399-72138418 MS.gene002145:CDS 50.0%
!!! TGCACGGTTTTCTGAGATCG+GGG + chr2.2:72138400-72138419 MS.gene002145:CDS 50.0%
AGCCTTGCCCTCCGACATAA+TGG - chr2.2:72137293-72137312 None:intergenic 55.0%
CAGTCCCCGGAGGACATATA+TGG - chr2.2:72138112-72138131 None:intergenic 55.0%
CATATATGTCCTCCGGGGAC+TGG + chr2.2:72138110-72138129 MS.gene002145:CDS 55.0%
CTGCCCATATATGTCCTCCG+GGG + chr2.2:72138105-72138124 MS.gene002145:CDS 55.0%
GATCGGGGGATCCTGTAATG+AGG + chr2.2:72138415-72138434 MS.gene002145:CDS 55.0%
GCTAATTCACCGTTCCCTGG+AGG + chr2.2:72138319-72138338 MS.gene002145:CDS 55.0%
GTATCGCCAAAGGTCCCATC+TGG + chr2.2:72138161-72138180 MS.gene002145:CDS 55.0%
TCCTCTCTGCTAAGAGGACC+AGG + chr2.2:72137482-72137501 MS.gene002145:CDS 55.0%
TCCTGGTCCTCTTAGCAGAG+AGG - chr2.2:72137486-72137505 None:intergenic 55.0%
TGGTCCTCTTAGCAGAGAGG+AGG - chr2.2:72137483-72137502 None:intergenic 55.0%
TGTCGGAGGGCAAGGCTTAA+TGG + chr2.2:72137296-72137315 MS.gene002145:intron 55.0%
! ATATGGGCAGAAGTGCAGGG+AGG - chr2.2:72138095-72138114 None:intergenic 55.0%
!! GGTGCTGGTTGAGATGGTGT+CGG - chr2.2:72138599-72138618 None:intergenic 55.0%
!!! GCACGGTTTTCTGAGATCGG+GGG + chr2.2:72138401-72138420 MS.gene002145:CDS 55.0%
ATGATTCCGTCTCGTGGCCC+TGG - chr2.2:72136857-72136876 None:intergenic 60.0%
CTTCCACCCAACCACCTGCT+GGG + chr2.2:72138570-72138589 MS.gene002145:CDS 60.0%
GGAACGAGTCCTCCAGGGAA+CGG - chr2.2:72138331-72138350 None:intergenic 60.0%
GTTCCCTGGAGGACTCGTTC+CGG + chr2.2:72138330-72138349 MS.gene002145:CDS 60.0%
TTCCACCCAACCACCTGCTG+GGG + chr2.2:72138571-72138590 MS.gene002145:CDS 60.0%
TTTCAGCCAGGGCCACGAGA+CGG + chr2.2:72136848-72136867 MS.gene002145:intron 60.0%
!! TAGCTCTGGCTGTCCGGGAA+CGG - chr2.2:72138303-72138322 None:intergenic 60.0%
!! TCGGGTGGTGCTGGTTGAGA+TGG - chr2.2:72138605-72138624 None:intergenic 60.0%
GCTCCGGAACGAGTCCTCCA+GGG - chr2.2:72138336-72138355 None:intergenic 65.0%
GCTTCCACCCAACCACCTGC+TGG + chr2.2:72138569-72138588 MS.gene002145:CDS 65.0%
TGCTCCGGAACGAGTCCTCC+AGG - chr2.2:72138337-72138356 None:intergenic 65.0%
TGCTCGGAACTCGAGGCCCA+AGG + chr2.2:72138222-72138241 MS.gene002145:CDS 65.0%
CGGCTCGTCTTCCCAGTCCC+CGG - chr2.2:72138125-72138144 None:intergenic 70.0%
CTCGTCTTCCCAGTCCCCGG+AGG - chr2.2:72138122-72138141 None:intergenic 70.0%
CGCCCCAGCAGGTGGTTGGG+TGG - chr2.2:72138576-72138595 None:intergenic 75.0%
CGGCGCCCCAGCAGGTGGTT+GGG - chr2.2:72138579-72138598 None:intergenic 75.0%
GATGCGGGCGGCTCCGTTCC+CGG + chr2.2:72138287-72138306 MS.gene002145:CDS 75.0%
TCGGCGCCCCAGCAGGTGGT+TGG - chr2.2:72138580-72138599 None:intergenic 75.0%
!! GATGGTGTCGGCGCCCCAGC+AGG - chr2.2:72138587-72138606 None:intergenic 75.0%
!!! GGTGTCGGCGCCCCAGCAGG+TGG - chr2.2:72138584-72138603 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 72136387 72138760 72136387 ID=MS.gene002145
chr2.2 mRNA 72136387 72138760 72136387 ID=MS.gene002145.t1;Parent=MS.gene002145
chr2.2 exon 72136387 72136737 72136387 ID=MS.gene002145.t1.exon1;Parent=MS.gene002145.t1
chr2.2 CDS 72136387 72136737 72136387 ID=cds.MS.gene002145.t1;Parent=MS.gene002145.t1
chr2.2 exon 72136854 72136941 72136854 ID=MS.gene002145.t1.exon2;Parent=MS.gene002145.t1
chr2.2 CDS 72136854 72136941 72136854 ID=cds.MS.gene002145.t1;Parent=MS.gene002145.t1
chr2.2 exon 72137455 72137587 72137455 ID=MS.gene002145.t1.exon3;Parent=MS.gene002145.t1
chr2.2 CDS 72137455 72137587 72137455 ID=cds.MS.gene002145.t1;Parent=MS.gene002145.t1
chr2.2 exon 72138049 72138760 72138049 ID=MS.gene002145.t1.exon4;Parent=MS.gene002145.t1
chr2.2 CDS 72138049 72138760 72138049 ID=cds.MS.gene002145.t1;Parent=MS.gene002145.t1
Gene Sequence

>MS.gene002145

ATGTCTTTCCGTAGCATAGTTCGTGACGTTCGTGATAGTTTTGGGAGCTTATCTAGGCGTAGTTTTGATGTCAAACTTACTGGCCATCATCATAGAGGAAAGTCTCATGGTTCTGTACAGGATTTGCATGATCAGCCTCTTGTAATTCAAAATAGTTGCTGGGCGAACTTACCCCCAGAATTACTATTTGATGTCATTCGGCGACTAGAGGAGAGTGAGAATACTTGGCCTTCAAGGAAGCATGTAGTTGCATGTGCTTCTGTTTGCCAGTCTTGGAGGAATATGTGCAAGGACATTGTTAAGAGCCCAGAGTTCTGCGGCAAACTAACATTCCCTGTGTCCCTGAAACAGGTTAGTTGCAGATTATCTCACTTTTTATTTATTAAGAATCAATTACATTTATGTTGAAATAACAACAATAGTTAAGGTAGCAACATGCTGACTTTGTCGTTCGTTTCACTTTTCAGCCAGGGCCACGAGACGGAATCATTCAATGCTTTATCAAAAGGGATAAATCTAACTTAACATATCACCTATTCCTTTGTCTGAGCCCTGGTAAACACTCTTCGCTTACGCCTAGTTACCTAGTGGGTATATATGGTTTTATGTTCAGATGCTTCTGGTGTGATTGTGTAAATTTGTTCTTATGTAATGTTTCAAAATTTAAGCAGTTTAGGTTTTTTAAGTTACTGCTATATGAATATTTGTGCATGTGTTGTTTGTCGCTGCACGTGAAGATGTATGCATCGATGTTTAATTTGAGATCTACTTTGGATGACACTTGTAATAGTGTGACTTGTGCCGATGCATAAAAATGAGATGTTTCTATAACCGTTGTCATTCCATGAATATTTATTTCCCTTTGTGATTGATTCTAGGAGTCTTGGATATTCTGTTTGATATCCATTATGTCGGAGGGCAAGGCTTAATGGTCTATTTTGTCGTTTTATATTAATTATTTTAGCAACATTACTTCTAAAGTTTTGATGCTAGGGAAGTTGTCTACAGCATCTTGAACTCATTTTAGTTTTGTTCCTGCTTCAATTTTATTTACTTTGACTTTTGCAGCTTTGTTAGTTGAAAATGGAAAATTCCTCCTCTCTGCTAAGAGGACCAGGAGAACAACCTACACTGAGTATGTTATTTCAATGGATGCTGACAACATCTCTAGATCAAGTAATACTTACATTGGAAAACTGAGGTAAATTTACTACTTGCAAATGTTGATCCTTCTCTCATCTACTATGTTAAAAACTTCTTTTCGGTGTCTATAAAAATATACTGTTAAAACTACTATGAATTATGATTCCGAGTTTCCGACTATCAAAACGCATTTTAACTCCCAAAAGTAAAACAGAAGAATCAATGATCTCGCAAAGTTGCAATGAATTTCAGTTGGTTTATGATTAAGTACTTCTTTTATCGTCATATGTTTAGGTGCACGAAATTATAATTATACAAAAATGTGTTGTAACTGTTATTATCGGAGGTAATAAATAGTCTAGGGTTCTTTATTCCATAAATAATTTTCCACTTATGTGATGCTGATATTCCTTGTTTGCTATTCATATCTTCAAGACCATTTGCTGAGTTTTTTCTGTTCTATTTTATATCTATGTCTTCTCACTTGCAAGATTATTTGTTATTATTTTGATACACCAGATCAAACTTTCTTGGAACAAAGTTCATCATATATGATACACAGCCTCCCTGCACTTCTGCCCATATATGTCCTCCGGGGACTGGGAAGACGAGCCGTAGATTTTCTAAAAAGGTATCGCCAAAGGTCCCATCTGGGAGTTACAACATAGCTCAGGTAACATATGAACTAAATGTGCTCGGAACTCGAGGCCCAAGGAAGATGCATTGCATTATGCATTCAATACCAAATTCAGCACTTGATGCGGGCGGCTCCGTTCCCGGACAGCCAGAGCTAATTCACCGTTCCCTGGAGGACTCGTTCCGGAGCATCTCATTTTCGAAGTCTCTAGATCATTCAATCGAGTTCAGCAGTGCACGGTTTTCTGAGATCGGGGGATCCTGTAATGAGGATGATGACGGCAAGATGAGACCTTTGGTTCTGAAAAACAAGCCTCCAAGATGGCATGAACAATTACAGTGCTGGTGCCTTAATTTTCGGGGACGAGTAACTGTTGCATCTGTTAAAAACTTTCAGCTGATTGCTTCCACCCAACCACCTGCTGGGGCGCCGACACCATCTCAACCAGCACCACCCGAACATGATAAGATTATTCTGCAATTCGGCAAAGTTGGGAAAGATATGTTCACCATGGATTATCGATATCCTTTATCTGCATTCCAAGCTTTTGCAATATGCTTAAGCAGCTTTGACACCAAGTTGGCTTGTGAATAG
Protein sequence

>MS.gene002145.t1

MSFRSIVRDVRDSFGSLSRRSFDVKLTGHHHRGKSHGSVQDLHDQPLVIQNSCWANLPPELLFDVIRRLEESENTWPSRKHVVACASVCQSWRNMCKDIVKSPEFCGKLTFPVSLKQPGPRDGIIQCFIKRDKSNLTYHLFLCLSPALLVENGKFLLSAKRTRRTTYTEYVISMDADNISRSSNTYIGKLRSNFLGTKFIIYDTQPPCTSAHICPPGTGKTSRRFSKKVSPKVPSGSYNIAQVTYELNVLGTRGPRKMHCIMHSIPNSALDAGGSVPGQPELIHRSLEDSFRSISFSKSLDHSIEFSSARFSEIGGSCNEDDDGKMRPLVLKNKPPRWHEQLQCWCLNFRGRVTVASVKNFQLIASTQPPAGAPTPSQPAPPEHDKIILQFGKVGKDMFTMDYRYPLSAFQAFAICLSSFDTKLACE