AlfalfaGEDB | MS.gene002459

M. sativa cultivar XinJiangDaYe / MS.gene002459

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF07714.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002459.t1	RZB81588.1	87.8	49	6	0	1	49	360	408	8.50E-16	92

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002459.t1	Q9ZQ31	55.6	45	20	0	1	45	301	345	5.4e-11	67.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002459.t1	A0A2P2LRB9	89.8	49	5	0	1	49	364	412	4.7e-16	92.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002459.t1	MTR_2g013900	93.617	47	3	1	47	334	380	2.90e-25	95.5
MS.gene002459.t1	MTR_4g088750	91.111	45	4	1	45	373	417	9.26e-24	91.7
MS.gene002459.t1	MTR_4g088755	82.222	45	8	1	45	212	256	6.54e-22	85.5
MS.gene002459.t1	MTR_4g088745	82.222	45	8	1	45	217	261	6.82e-22	85.5
MS.gene002459.t1	MTR_8g100085	55.556	45	20	1	45	304	348	2.19e-15	67.8
MS.gene002459.t1	MTR_3g034100	55.556	45	20	1	45	253	297	3.09e-15	67.4
MS.gene002459.t1	MTR_4g132860	57.778	45	19	1	45	251	295	9.05e-14	63.2
MS.gene002459.t1	MTR_1g026160	51.064	47	23	1	47	456	502	1.21e-12	60.1
MS.gene002459.t1	MTR_3g078000	55.556	45	20	1	45	228	272	1.66e-12	59.7
MS.gene002459.t1	MTR_1g026160	51.064	47	23	1	47	311	357	1.74e-12	59.7
MS.gene002459.t1	MTR_1g026160	51.064	47	23	1	47	309	355	1.77e-12	59.7
MS.gene002459.t1	MTR_1g026160	51.064	47	23	1	47	311	357	3.96e-12	58.5
MS.gene002459.t1	MTR_1g026160	51.111	45	22	1	45	456	500	5.04e-12	58.5
MS.gene002459.t1	MTR_1g026160	51.111	45	22	1	45	311	355	6.58e-12	58.2
MS.gene002459.t1	MTR_1g026160	51.111	45	22	1	45	309	353	6.64e-12	58.2
MS.gene002459.t1	MTR_4g105770	48.889	45	23	1	45	229	273	1.22e-11	57.4
MS.gene002459.t1	MTR_1g113960	51.064	47	23	1	47	467	513	1.54e-11	57.0
MS.gene002459.t1	MTR_1g113960	51.064	47	23	1	47	467	513	1.54e-11	57.0
MS.gene002459.t1	MTR_7g072710	51.163	43	21	3	45	225	267	2.41e-11	56.6
MS.gene002459.t1	MTR_7g072710	51.163	43	21	3	45	188	230	3.31e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002459.t1	AT5G58950	82.222	45	8	0	1	45	380	424	2.16e-21	85.1
MS.gene002459.t1	AT2G24360	55.556	45	20	0	1	45	301	345	1.06e-15	68.9
MS.gene002459.t1	AT4G31170	55.556	45	20	0	1	45	302	346	2.76e-15	67.8
MS.gene002459.t1	AT4G31170	55.556	45	20	0	1	45	302	346	2.76e-15	67.8
MS.gene002459.t1	AT4G31170	55.556	45	20	0	1	45	302	346	2.76e-15	67.8
MS.gene002459.t1	AT4G31170	55.556	45	20	0	1	45	302	346	2.76e-15	67.8
MS.gene002459.t1	AT4G31170	55.556	45	20	0	1	45	302	346	2.76e-15	67.8
MS.gene002459.t1	AT4G31170	55.556	45	20	0	1	45	302	346	2.76e-15	67.8
MS.gene002459.t1	AT4G31170	55.556	45	20	0	1	45	302	346	2.76e-15	67.8
MS.gene002459.t1	AT1G62400	57.778	45	19	0	1	45	257	301	5.87e-14	63.9
MS.gene002459.t1	AT3G46930	59.574	47	18	1	1	46	275	321	2.08e-13	62.4
MS.gene002459.t1	AT3G46930	59.574	47	18	1	1	46	336	382	2.25e-13	62.4
MS.gene002459.t1	AT3G46930	59.574	47	18	1	1	46	371	417	2.27e-13	62.4
MS.gene002459.t1	AT3G46930	59.574	47	18	1	1	46	371	417	2.28e-13	62.4
MS.gene002459.t1	AT3G46930	58.696	46	18	1	1	45	336	381	8.31e-13	60.5
MS.gene002459.t1	AT3G46930	58.696	46	18	1	1	45	371	416	8.32e-13	60.5
MS.gene002459.t1	AT4G38470	51.064	47	23	0	1	47	456	502	2.58e-12	59.3
MS.gene002459.t1	AT4G38470	51.064	47	23	0	1	47	456	502	1.25e-11	57.4
MS.gene002459.t1	AT4G38470	51.064	47	23	0	1	47	456	502	1.25e-11	57.4
MS.gene002459.t1	AT5G01850	51.163	43	21	0	3	45	198	240	2.46e-11	56.6
MS.gene002459.t1	AT5G01850	51.163	43	21	0	3	45	222	264	3.03e-11	56.2

Find 15 sgRNAs with CRISPR-Local

Find 19 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAGGTTGACGTGTACAGTTT+TGG	0.329020	2.1:-71102483	MS.gene002459:CDS
ATGATCAAACGGAAATCATA+TGG	0.389393	2.1:-71102510	MS.gene002459:CDS
ATCCTCGTAGGGAATTGTTC+CGG	0.475958	2.1:+71102437	None:intergenic
ATTAAAAGCCAGGAAAATAC+AGG	0.479674	2.1:+71102313	None:intergenic
GCTTATCTTATGGGAAATGT+TGG	0.502118	2.1:-71102460	MS.gene002459:CDS
TCACAACAGCAAAAGCAGCC+TGG	0.502978	2.1:+71102403	None:intergenic
CGAGGATATGAATACAATCC+AGG	0.512884	2.1:-71102421	MS.gene002459:CDS
ATCTTATGGGAAATGTTGGC+CGG	0.521300	2.1:-71102456	MS.gene002459:CDS
ACGGAAATCATATGGGAGAA+AGG	0.527210	2.1:-71102502	MS.gene002459:CDS
TGAACAATTCATTAAAAGCC+AGG	0.531183	2.1:+71102303	None:intergenic
TGGCACCTGAAATGATCAAA+CGG	0.540411	2.1:-71102521	None:intergenic
TGATCAAACGGAAATCATAT+GGG	0.554649	2.1:-71102509	MS.gene002459:CDS
GATTGTATTCATATCCTCGT+AGG	0.559235	2.1:+71102425	None:intergenic
ATTGTATTCATATCCTCGTA+GGG	0.595435	2.1:+71102426	None:intergenic
GGCCGGAACAATTCCCTACG+AGG	0.692539	2.1:-71102439	MS.gene002459:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	ATGATCAAACGGAAATCATA+TGG	-	chr2.1:71102314-71102333	MS.gene002459:CDS	30.0%
	ATTGTATTCATATCCTCGTA+GGG	+	chr2.1:71102401-71102420	None:intergenic	30.0%
	CATTATAGTACTCTTTGTGT+AGG	-	chr2.1:71102487-71102506	MS.gene002459:CDS	30.0%
	TAGATGATTTGAACACACAA+AGG	+	chr2.1:71102459-71102478	None:intergenic	30.0%
	TGATCAAACGGAAATCATAT+GGG	-	chr2.1:71102315-71102334	MS.gene002459:CDS	30.0%
	GATTGTATTCATATCCTCGT+AGG	+	chr2.1:71102402-71102421	None:intergenic	35.0%
	GCTTATCTTATGGGAAATGT+TGG	-	chr2.1:71102364-71102383	MS.gene002459:intron	35.0%
!!!	ACAGTTTTGGGCTTATCTTA+TGG	-	chr2.1:71102354-71102373	MS.gene002459:intron	35.0%
!!!	CAGTTTTGGGCTTATCTTAT+GGG	-	chr2.1:71102355-71102374	MS.gene002459:intron	35.0%
!!!	TGCTTTTGCTGTTGTGAATA+AGG	-	chr2.1:71102427-71102446	MS.gene002459:CDS	35.0%
	ACGGAAATCATATGGGAGAA+AGG	-	chr2.1:71102322-71102341	MS.gene002459:intron	40.0%
	ATCTTATGGGAAATGTTGGC+CGG	-	chr2.1:71102368-71102387	MS.gene002459:intron	40.0%
	CGAGGATATGAATACAATCC+AGG	-	chr2.1:71102403-71102422	MS.gene002459:CDS	40.0%
!	AAGGTTGACGTGTACAGTTT+TGG	-	chr2.1:71102341-71102360	MS.gene002459:intron	40.0%
!	AGGTTGACGTGTACAGTTTT+GGG	-	chr2.1:71102342-71102361	MS.gene002459:intron	40.0%
	ATCCTCGTAGGGAATTGTTC+CGG	+	chr2.1:71102390-71102409	None:intergenic	45.0%
!	GTGTAGGTCCTGTATTTTCC+TGG	-	chr2.1:71102503-71102522	MS.gene002459:CDS	45.0%
	TCACAACAGCAAAAGCAGCC+TGG	+	chr2.1:71102424-71102443	None:intergenic	50.0%
	GGCCGGAACAATTCCCTACG+AGG	-	chr2.1:71102385-71102404	MS.gene002459:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	71102314	71102532	71102314	ID=MS.gene002459
chr2.1	mRNA	71102314	71102532	71102314	ID=MS.gene002459.t1;Parent=MS.gene002459
chr2.1	exon	71102398	71102532	71102398	ID=MS.gene002459.t1.exon1;Parent=MS.gene002459.t1
chr2.1	CDS	71102398	71102532	71102398	ID=cds.MS.gene002459.t1;Parent=MS.gene002459.t1
chr2.1	exon	71102314	71102337	71102314	ID=MS.gene002459.t1.exon2;Parent=MS.gene002459.t1
chr2.1	CDS	71102314	71102337	71102314	ID=cds.MS.gene002459.t1;Parent=MS.gene002459.t1

Gene Sequence	>MS.gene002459 ATGATCAAACGGAAATCATATGGGAGAAAGGTTGACGTGTACAGTTTTGGGCTTATCTTATGGGAAATGTTGGCCGGAACAATTCCCTACGAGGATATGAATACAATCCAGGCTGCTTTTGCTGTTGTGAATAAGGTATGTTCCTTTGTGTGTTCAAATCATCTATATAACTTCATTATAGTACTCTTTGTGTAGGTCCTGTATTTTCCTGGCTTTTAA
Protein sequence	>MS.gene002459.t1 MIKRKSYGRKVDVYSFGLILWEMLAGTIPYEDMNTIQAAFAVVNKVLYFPGF