AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002517

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002517.t1 MTR_4g088125 73.617 470 109 3 3 461 5 470 0.0 710
MS.gene002517.t1 MTR_4g079240 45.783 498 201 10 3 437 6 497 5.64e-137 404
MS.gene002517.t1 MTR_2g014870 93.701 127 5 2 1 124 1 127 8.64e-76 234
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002517.t1 AT1G28520 64.592 466 140 6 3 450 6 464 0.0 586
MS.gene002517.t1 AT1G28520 64.592 466 140 6 3 450 6 464 0.0 586
MS.gene002517.t1 AT1G28520 64.592 466 140 6 3 450 6 464 0.0 586
MS.gene002517.t1 AT1G28520 64.592 466 140 6 3 450 6 464 0.0 586
MS.gene002517.t1 AT1G28520 61.803 466 137 7 3 450 6 448 0.0 548
MS.gene002517.t1 AT2G42400 48.864 440 178 10 10 416 9 434 1.84e-123 367

Find 115 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTCATCACTTGCTTTC+TGG -0.000768 2.1:+70328601 None:intergenic
GTTGCCGCTCCCTCGCATTC+TGG 0.120554 2.1:+70328881 None:intergenic
GTATGTACTCATCCATTAAT+AGG 0.244748 2.1:+70329323 None:intergenic
TTGGAACTAAAGCTCGTTAA+TGG 0.245316 2.1:-70328467 MS.gene002517:CDS
TCAGCATGATTCCCTATTAA+TGG 0.250839 2.1:-70329335 MS.gene002517:CDS
CATAAAGATGCCACTCAAAA+TGG 0.277276 2.1:+70328531 None:intergenic
CGAGTTGTTAGTTGATTTAC+TGG 0.280780 2.1:+70329282 None:intergenic
TTGCCGCTCCCTCGCATTCT+GGG 0.309111 2.1:+70328882 None:intergenic
AGGTCGTTGCCTGATTATAA+TGG 0.319828 2.1:-70328641 MS.gene002517:CDS
GGTAGATAGGTAGGTATATA+AGG 0.325810 2.1:+70329145 None:intergenic
GTCAGCCTTGGCATTAGGTT+TGG 0.334729 2.1:+70328328 None:intergenic
GTCTGCATATTCTGATCATT+AGG 0.347297 2.1:+70329860 None:intergenic
GAGTACATACACTCCATCTT+AGG 0.353453 2.1:-70329310 MS.gene002517:CDS
CCCATGTCAGCCTTGGCATT+AGG 0.366697 2.1:+70328323 None:intergenic
AGGGCAGATGCTTGGTAGAT+AGG 0.368518 2.1:+70329132 None:intergenic
AAGCAAGTGATGAAAGAGTT+TGG 0.371141 2.1:-70328596 MS.gene002517:CDS
TCCTGCTTCTTCTTTGCAGC+AGG 0.376005 2.1:-70330121 MS.gene002517:intron
AGCAGAATGTCTGTTATCAT+AGG 0.386907 2.1:+70328361 None:intergenic
AGGTTCAAAATGTGCACTTT+GGG 0.394325 2.1:-70329092 MS.gene002517:CDS
ATCAATTGTTATCTTTGTCC+TGG 0.407827 2.1:+70328433 None:intergenic
CCTCCTCCTTCCGCTTTCCT+AGG 0.410735 2.1:-70329112 MS.gene002517:CDS
TGCCACTCAAAATGGTTTAG+TGG 0.430399 2.1:+70328539 None:intergenic
TCAAAATGGTTTAGTGGTTG+TGG 0.432899 2.1:+70328545 None:intergenic
CCTTCCACTCACGAAGCATC+TGG 0.436154 2.1:+70330163 None:intergenic
TCATGCTTAGGCACAGCTAA+TGG 0.436229 2.1:+70329884 None:intergenic
TAGGTTCAAAATGTGCACTT+TGG 0.438695 2.1:-70329093 MS.gene002517:CDS
CTGCAGTAGCCTACATATAT+CGG 0.447566 2.1:+70329937 None:intergenic
GGTCGTTGCCTGATTATAAT+GGG 0.452127 2.1:-70328640 MS.gene002517:CDS
TATGTACTCATCCATTAATA+GGG 0.456560 2.1:+70329324 None:intergenic
TGGAACTAAAGCTCGTTAAT+GGG 0.459138 2.1:-70328466 MS.gene002517:CDS
GCTGCTCTTGCTTTAAATGA+AGG 0.460368 2.1:-70329010 MS.gene002517:CDS
CATCCCCGCCCATTATAATC+AGG 0.465616 2.1:+70328632 None:intergenic
TGATCATTAGGTTCATGCTT+AGG 0.466635 2.1:+70329872 None:intergenic
TAGCCCCAGTTCTACGACCT+GGG 0.466781 2.1:-70328976 MS.gene002517:CDS
TGCTTTCTTACAGCTACCCT+TGG 0.466878 2.1:+70330314 None:intergenic
GTGATGCATGTGTGTTATAT+CGG 0.471671 2.1:-70328490 MS.gene002517:CDS
ACCTAATGCCAAGGCTGACA+TGG 0.476238 2.1:-70328324 MS.gene002517:CDS
CGTAGAACTGGGGCTATGCC+TGG 0.483033 2.1:+70328983 None:intergenic
TGATCTGCAGTTTGCTAGGA+AGG 0.490071 2.1:-70330229 MS.gene002517:CDS
CTGATTATAATGGGCGGGGA+TGG 0.493655 2.1:-70328631 MS.gene002517:CDS
TCCTAGCAAACTGCAGATCA+AGG 0.504979 2.1:+70330232 None:intergenic
GTTGCCTGATTATAATGGGC+GGG 0.508969 2.1:-70328636 MS.gene002517:CDS
TGGTTGTGGATCCATGTAGT+AGG 0.510429 2.1:+70328559 None:intergenic
CATCCCAGAATGCGAGGGAG+CGG 0.511232 2.1:-70328885 MS.gene002517:CDS
TATACTGCTTTCAGAGGCAC+AGG 0.514147 2.1:-70329190 MS.gene002517:CDS
TTTACTGGGAGCTCCTAAGA+TGG 0.517698 2.1:+70329297 None:intergenic
AACACCACCCATGTCAGCCT+TGG 0.521369 2.1:+70328316 None:intergenic
CCTAGGAAAGCGGAAGGAGG+AGG 0.522377 2.1:+70329112 None:intergenic
GAAGGAGGAGGGCAGATGCT+TGG 0.522630 2.1:+70329124 None:intergenic
CTCTCAACAAGATAGTCATA+TGG 0.523925 2.1:+70328266 None:intergenic
TGTGGATGTCGCGCTTCTTG+AGG 0.525115 2.1:-70330196 MS.gene002517:CDS
AGCATCACACAGGCTTATCA+AGG 0.532332 2.1:-70330295 MS.gene002517:CDS
CACAAGTTCAACCCTTCCGC+GGG 0.534065 2.1:+70329058 None:intergenic
CCAGATGCTTCGTGAGTGGA+AGG 0.543218 2.1:-70330163 MS.gene002517:CDS
TGATAACAGACATTCTGCTA+AGG 0.543724 2.1:-70328357 MS.gene002517:CDS
GGCACAGGCTTTAGTGAGGA+TGG 0.545649 2.1:-70329175 MS.gene002517:CDS
ACCTGCTGCAAAGAAGAAGC+AGG 0.547011 2.1:+70330120 None:intergenic
CATTCTCGACCGATATATGT+AGG 0.550090 2.1:-70329946 MS.gene002517:CDS
CAGATGCTTGGTAGATAGGT+AGG 0.551289 2.1:+70329136 None:intergenic
GCTTTAAATGAAGGACAACC+AGG 0.554170 2.1:-70329001 MS.gene002517:CDS
GCAGAATGTCTGTTATCATA+GGG 0.554543 2.1:+70328362 None:intergenic
AGTTAAGGATAATTTACTGT+TGG 0.554729 2.1:-70328945 MS.gene002517:CDS
ATAGCCCCAGTTCTACGACC+TGG 0.557433 2.1:-70328977 MS.gene002517:CDS
TGAGGTTTAGATGATTTCCA+AGG 0.559690 2.1:-70330331 None:intergenic
ATAGGGAATCATGCTGATGT+TGG 0.560720 2.1:+70329341 None:intergenic
TTAATGGGAAGAAGAGCTCC+AGG 0.561240 2.1:-70328451 MS.gene002517:CDS
TCCTTGATCTGCAGTTTGCT+AGG 0.561339 2.1:-70330233 MS.gene002517:CDS
CAGTAAATCAACTAACAACT+CGG 0.567663 2.1:-70329281 MS.gene002517:CDS
ACACAAGTTCAACCCTTCCG+CGG 0.569071 2.1:+70329057 None:intergenic
TGCAAAGAAGAAGCAGGAGA+AGG 0.571409 2.1:+70330126 None:intergenic
AAGTCTCCGTGGAATGCACC+TGG 0.578340 2.1:-70328854 MS.gene002517:intron
CTAGGAAAGCGGAAGGAGGA+GGG 0.578498 2.1:+70329113 None:intergenic
TGCATGTGTGTTATATCGGT+TGG 0.581993 2.1:-70328486 MS.gene002517:CDS
TTGCCTGATTATAATGGGCG+GGG 0.584990 2.1:-70328635 MS.gene002517:CDS
AAGTGATGAAAGAGTTTGGA+GGG 0.587404 2.1:-70328592 MS.gene002517:CDS
GACTGTTCAAGACCCGCGGA+AGG 0.587498 2.1:-70329070 MS.gene002517:CDS
GCTCTTAGTGCAAAGACAGA+AGG 0.588265 2.1:-70328920 MS.gene002517:CDS
GATAACAGACATTCTGCTAA+GGG 0.588460 2.1:-70328356 MS.gene002517:CDS
ACAAGATAGTCATATGGTGA+TGG 0.593941 2.1:+70328272 None:intergenic
CAGTTACTGATCTTCAGAAG+CGG 0.596139 2.1:-70328409 MS.gene002517:CDS
TTCACCAGATGCTTCGTGAG+TGG 0.596799 2.1:-70330167 MS.gene002517:CDS
AACTGTTATACTGCTTTCAG+AGG 0.607971 2.1:-70329196 MS.gene002517:CDS
CAGAGCCAAACCTAATGCCA+AGG 0.608334 2.1:-70328333 MS.gene002517:CDS
AGGAGAAGGCTCATTGAGCT+CGG 0.609324 2.1:+70330140 None:intergenic
AATCAACTAACAACTCGGAT+AGG 0.609346 2.1:-70329276 MS.gene002517:CDS
AGCCCCAGTTCTACGACCTG+GGG 0.611570 2.1:-70328975 MS.gene002517:CDS
CCTAATGCCAAGGCTGACAT+GGG 0.613097 2.1:-70328323 MS.gene002517:CDS
CTAATACCAGGTGCATTCCA+CGG 0.614138 2.1:+70328848 None:intergenic
TGTCGGCATCCCAGAATGCG+AGG 0.614404 2.1:-70328891 MS.gene002517:CDS
GCTACTGCAGCTCTGTGAAG+AGG 0.620067 2.1:-70329924 MS.gene002517:CDS
AAGACAGAAGGAAAAGATGT+CGG 0.620071 2.1:-70328908 MS.gene002517:CDS
GAGGTTTAGATGATTTCCAA+GGG 0.620877 2.1:-70330330 None:intergenic
CAGAGGCACAGGCTTTAGTG+AGG 0.620913 2.1:-70329179 MS.gene002517:CDS
AGGCACAGCTAATGGACTAG+TGG 0.628612 2.1:+70329892 None:intergenic
CGTTGCCTGATTATAATGGG+CGG 0.632470 2.1:-70328637 MS.gene002517:CDS
TTTGAACCTAGGAAAGCGGA+AGG 0.634334 2.1:+70329106 None:intergenic
GCTGAAGAGATCCTACTACA+TGG 0.634906 2.1:-70328570 MS.gene002517:CDS
CAACTAACAACTCGGATAGG+CGG 0.636833 2.1:-70329273 MS.gene002517:CDS
TCTCGAAGAGCATTTGAGAG+TGG 0.636901 2.1:-70328677 MS.gene002517:CDS
ACTGTTCAAGACCCGCGGAA+GGG 0.637959 2.1:-70329069 MS.gene002517:CDS
TTGGGACTGTTCAAGACCCG+CGG 0.641938 2.1:-70329074 MS.gene002517:CDS
GAAGCGCGACATCCACACTA+CGG 0.643778 2.1:+70330202 None:intergenic
TCACATGCAGATCATCTACA+TGG 0.644881 2.1:+70330259 None:intergenic
GAACCTAGGAAAGCGGAAGG+AGG 0.646171 2.1:+70329109 None:intergenic
TCTGTTATCATAGGGAAACT+CGG 0.647001 2.1:+70328370 None:intergenic
CGGCAACTGCAAAGTCTCCG+TGG 0.652685 2.1:-70328865 MS.gene002517:CDS
GTCGGCATCCCAGAATGCGA+GGG 0.657248 2.1:-70328890 MS.gene002517:CDS
TAGGAAGGAGAGCCGTAGTG+TGG 0.661628 2.1:-70330214 MS.gene002517:CDS
GAGTTGTTAGTTGATTTACT+GGG 0.663415 2.1:+70329283 None:intergenic
CAAGTGATGAAAGAGTTTGG+AGG 0.680936 2.1:-70328593 MS.gene002517:CDS
GCTGTAAGAAAGCATCACAC+AGG 0.691286 2.1:-70330305 MS.gene002517:CDS
ACACAGGCTTATCAAGGACA+AGG 0.694542 2.1:-70330289 MS.gene002517:CDS
AGAGTGGAAACAGAAAGCAA+AGG 0.717117 2.1:-70328661 MS.gene002517:CDS
GCCCCAGTTCTACGACCTGG+GGG 0.725582 2.1:-70328974 MS.gene002517:CDS
AATGCCAAGGCTGACATGGG+TGG 0.739584 2.1:-70328320 MS.gene002517:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTATTTTTTAGAGAAA+AGG + chr2.1:70329002-70329021 None:intergenic 10.0%
!! AAACACATAAATAGTTTAAT+AGG + chr2.1:70329766-70329785 None:intergenic 15.0%
!!! TTTTATTTTTTCTGTAGTAT+TGG - chr2.1:70328823-70328842 MS.gene002517:intron 15.0%
!! AAATCTGCATATTAAAGTTA+AGG + chr2.1:70328491-70328510 None:intergenic 20.0%
!! TAATTCATATACTAGAGTAA+GGG - chr2.1:70328894-70328913 MS.gene002517:CDS 20.0%
!! TTAATATGCAGATTTAATTG+TGG - chr2.1:70328496-70328515 MS.gene002517:CDS 20.0%
!! TTAATTCATATACTAGAGTA+AGG - chr2.1:70328893-70328912 MS.gene002517:CDS 20.0%
!!! GTTGTATTGTTTTTTATATG+TGG - chr2.1:70328973-70328992 MS.gene002517:CDS 20.0%
!!! TTATTTTCAATTGACAGAAA+TGG - chr2.1:70329029-70329048 MS.gene002517:CDS 20.0%
! AGTTAAGGATAATTTACTGT+TGG - chr2.1:70329623-70329642 MS.gene002517:intron 25.0%
! ATGATGCATAAGAACAAATT+GGG + chr2.1:70329121-70329140 None:intergenic 25.0%
! ATTTAATTGTGGATTTGTGA+TGG - chr2.1:70328507-70328526 MS.gene002517:CDS 25.0%
! GAGAAAATTGGTATTAACTA+AGG + chr2.1:70329083-70329102 None:intergenic 25.0%
! TATGATGCATAAGAACAAAT+TGG + chr2.1:70329122-70329141 None:intergenic 25.0%
! TATGATTGTAACAAGTTTCT+TGG - chr2.1:70329186-70329205 MS.gene002517:CDS 25.0%
! TATGTACTCATCCATTAATA+GGG + chr2.1:70329247-70329266 None:intergenic 25.0%
!!! CGACATTTTGATTCAAAATT+AGG + chr2.1:70329321-70329340 None:intergenic 25.0%
AACTTGAATGGCAATATTTG+TGG - chr2.1:70328539-70328558 MS.gene002517:CDS 30.0%
ATCAATTGTTATCTTTGTCC+TGG + chr2.1:70330138-70330157 None:intergenic 30.0%
CAGTAAATCAACTAACAACT+CGG - chr2.1:70329287-70329306 MS.gene002517:CDS 30.0%
CTTTGTGATTTGATCATAAC+TGG - chr2.1:70328794-70328813 MS.gene002517:intron 30.0%
GTATGTACTCATCCATTAAT+AGG + chr2.1:70329248-70329267 None:intergenic 30.0%
! ATTTGAGTTTGCCATGTTTA+TGG - chr2.1:70328573-70328592 MS.gene002517:CDS 30.0%
! GAGTTGTTAGTTGATTTACT+GGG + chr2.1:70329288-70329307 None:intergenic 30.0%
! TTTGAGTTTGCCATGTTTAT+GGG - chr2.1:70328574-70328593 MS.gene002517:CDS 30.0%
! TTTGATCTTTTTGTTCTCGA+GGG - chr2.1:70329834-70329853 MS.gene002517:intron 30.0%
AACTGTTATACTGCTTTCAG+AGG - chr2.1:70329372-70329391 MS.gene002517:intron 35.0%
AAGACAGAAGGAAAAGATGT+CGG - chr2.1:70329660-70329679 MS.gene002517:intron 35.0%
AAGCAAGTGATGAAAGAGTT+TGG - chr2.1:70329972-70329991 MS.gene002517:intron 35.0%
AAGTGATGAAAGAGTTTGGA+GGG - chr2.1:70329976-70329995 MS.gene002517:intron 35.0%
AATCAACTAACAACTCGGAT+AGG - chr2.1:70329292-70329311 MS.gene002517:CDS 35.0%
ACAAGATAGTCATATGGTGA+TGG + chr2.1:70330299-70330318 None:intergenic 35.0%
AGCAGAATGTCTGTTATCAT+AGG + chr2.1:70330210-70330229 None:intergenic 35.0%
AGGTTCAAAATGTGCACTTT+GGG - chr2.1:70329476-70329495 MS.gene002517:intron 35.0%
CATAAAGATGCCACTCAAAA+TGG + chr2.1:70330040-70330059 None:intergenic 35.0%
CATGTTTATGGGTGATGATA+GGG - chr2.1:70328585-70328604 MS.gene002517:CDS 35.0%
CTCTCAACAAGATAGTCATA+TGG + chr2.1:70330305-70330324 None:intergenic 35.0%
GATAACAGACATTCTGCTAA+GGG - chr2.1:70330212-70330231 MS.gene002517:CDS 35.0%
GCAGAATGTCTGTTATCATA+GGG + chr2.1:70330209-70330228 None:intergenic 35.0%
GCATTCTACATTCTAATACC+AGG + chr2.1:70329735-70329754 None:intergenic 35.0%
GGTAGATAGGTAGGTATATA+AGG + chr2.1:70329426-70329445 None:intergenic 35.0%
GTCTGCATATTCTGATCATT+AGG + chr2.1:70328711-70328730 None:intergenic 35.0%
GTGATGCATGTGTGTTATAT+CGG - chr2.1:70330078-70330097 MS.gene002517:intron 35.0%
TAGGTTCAAAATGTGCACTT+TGG - chr2.1:70329475-70329494 MS.gene002517:intron 35.0%
TCAAAATGGTTTAGTGGTTG+TGG + chr2.1:70330026-70330045 None:intergenic 35.0%
TCAGCATGATTCCCTATTAA+TGG - chr2.1:70329233-70329252 MS.gene002517:CDS 35.0%
TCTGTTATCATAGGGAAACT+CGG + chr2.1:70330201-70330220 None:intergenic 35.0%
TGATAACAGACATTCTGCTA+AGG - chr2.1:70330211-70330230 MS.gene002517:CDS 35.0%
TGATCATTAGGTTCATGCTT+AGG + chr2.1:70328699-70328718 None:intergenic 35.0%
TGGAACTAAAGCTCGTTAAT+GGG - chr2.1:70330102-70330121 MS.gene002517:intron 35.0%
TGGACACATGTAAACTTGAA+TGG - chr2.1:70328527-70328546 MS.gene002517:CDS 35.0%
TGTATGAATAATCGCTGAAG+AGG + chr2.1:70328773-70328792 None:intergenic 35.0%
TTGGAACTAAAGCTCGTTAA+TGG - chr2.1:70330101-70330120 MS.gene002517:intron 35.0%
! AAAGTGCACATTTTGAACCT+AGG + chr2.1:70329476-70329495 None:intergenic 35.0%
! AACCACTAAACCATTTTGAG+TGG - chr2.1:70330027-70330046 MS.gene002517:intron 35.0%
! ACATTTTGAACCTAGGAAAG+CGG + chr2.1:70329469-70329488 None:intergenic 35.0%
! CGAGTTGTTAGTTGATTTAC+TGG + chr2.1:70329289-70329308 None:intergenic 35.0%
! CTTTGATCTTTTTGTTCTCG+AGG - chr2.1:70329833-70329852 MS.gene002517:intron 35.0%
! TGAGTTGTTGGTGAGAAAAT+TGG + chr2.1:70329095-70329114 None:intergenic 35.0%
! TTGATCTTTTTGTTCTCGAG+GGG - chr2.1:70329835-70329854 MS.gene002517:CDS 35.0%
AATGGAGAGCAAGGATTTGA+TGG - chr2.1:70329047-70329066 MS.gene002517:CDS 40.0%
AGAGTGGAAACAGAAAGCAA+AGG - chr2.1:70329907-70329926 MS.gene002517:CDS 40.0%
AGGTCGTTGCCTGATTATAA+TGG - chr2.1:70329927-70329946 MS.gene002517:CDS 40.0%
ATAATCGCTGAAGAGGAGAA+AGG + chr2.1:70328766-70328785 None:intergenic 40.0%
ATAGGGAATCATGCTGATGT+TGG + chr2.1:70329230-70329249 None:intergenic 40.0%
ATTGACAGAAATGGAGAGCA+AGG - chr2.1:70329038-70329057 MS.gene002517:CDS 40.0%
CAAGTGATGAAAGAGTTTGG+AGG - chr2.1:70329975-70329994 MS.gene002517:intron 40.0%
CAGTTACTGATCTTCAGAAG+CGG - chr2.1:70330159-70330178 MS.gene002517:CDS 40.0%
CATTCTCGACCGATATATGT+AGG - chr2.1:70328622-70328641 MS.gene002517:CDS 40.0%
CCATGTTTATGGGTGATGAT+AGG - chr2.1:70328584-70328603 MS.gene002517:CDS 40.0%
CCTATCATCACCCATAAACA+TGG + chr2.1:70328587-70328606 None:intergenic 40.0%
CTCTTTCATCACTTGCTTTC+TGG + chr2.1:70329970-70329989 None:intergenic 40.0%
CTGCAGTAGCCTACATATAT+CGG + chr2.1:70328634-70328653 None:intergenic 40.0%
GAGTACATACACTCCATCTT+AGG - chr2.1:70329258-70329277 MS.gene002517:CDS 40.0%
GGTCGTTGCCTGATTATAAT+GGG - chr2.1:70329928-70329947 MS.gene002517:CDS 40.0%
TACTAGAGTAAGGGATGCTT+TGG - chr2.1:70328903-70328922 MS.gene002517:CDS 40.0%
TCACATGCAGATCATCTACA+TGG + chr2.1:70328312-70328331 None:intergenic 40.0%
TGCATGTGTGTTATATCGGT+TGG - chr2.1:70330082-70330101 MS.gene002517:intron 40.0%
TGCCACTCAAAATGGTTTAG+TGG + chr2.1:70330032-70330051 None:intergenic 40.0%
! GCTGCTCTTGCTTTAAATGA+AGG - chr2.1:70329558-70329577 MS.gene002517:intron 40.0%
! GCTTTAAATGAAGGACAACC+AGG - chr2.1:70329567-70329586 MS.gene002517:intron 40.0%
! TGATCTTTTTGTTCTCGAGG+GGG - chr2.1:70329836-70329855 MS.gene002517:CDS 40.0%
!! CAAATTGGGCTTTGAGTTGT+TGG + chr2.1:70329107-70329126 None:intergenic 40.0%
ACACAGGCTTATCAAGGACA+AGG - chr2.1:70328279-70328298 MS.gene002517:CDS 45.0%
ACCTGGGGGAATTGAAGTTA+AGG - chr2.1:70329608-70329627 MS.gene002517:intron 45.0%
AGCATCACACAGGCTTATCA+AGG - chr2.1:70328273-70328292 MS.gene002517:CDS 45.0%
CAACTAACAACTCGGATAGG+CGG - chr2.1:70329295-70329314 MS.gene002517:CDS 45.0%
CAGATGCTTGGTAGATAGGT+AGG + chr2.1:70329435-70329454 None:intergenic 45.0%
CTAATACCAGGTGCATTCCA+CGG + chr2.1:70329723-70329742 None:intergenic 45.0%
GCTCTTAGTGCAAAGACAGA+AGG - chr2.1:70329648-70329667 MS.gene002517:intron 45.0%
GCTGAAGAGATCCTACTACA+TGG - chr2.1:70329998-70330017 MS.gene002517:intron 45.0%
GTTTATGGGTGATGATAGGG+TGG - chr2.1:70328588-70328607 MS.gene002517:CDS 45.0%
TCATGCTTAGGCACAGCTAA+TGG + chr2.1:70328687-70328706 None:intergenic 45.0%
TCCTAGCAAACTGCAGATCA+AGG + chr2.1:70328339-70328358 None:intergenic 45.0%
TCCTTAACTTCAATTCCCCC+AGG + chr2.1:70329612-70329631 None:intergenic 45.0%
TGCAAAGAAGAAGCAGGAGA+AGG + chr2.1:70328445-70328464 None:intergenic 45.0%
TGCTTTCTTACAGCTACCCT+TGG + chr2.1:70328257-70328276 None:intergenic 45.0%
TGGTTGTGGATCCATGTAGT+AGG + chr2.1:70330012-70330031 None:intergenic 45.0%
TTAATGGGAAGAAGAGCTCC+AGG - chr2.1:70330117-70330136 MS.gene002517:intron 45.0%
TTTACTGGGAGCTCCTAAGA+TGG + chr2.1:70329274-70329293 None:intergenic 45.0%
TTTGAACCTAGGAAAGCGGA+AGG + chr2.1:70329465-70329484 None:intergenic 45.0%
! CGTTGCCTGATTATAATGGG+CGG - chr2.1:70329931-70329950 MS.gene002517:CDS 45.0%
! TATACTGCTTTCAGAGGCAC+AGG - chr2.1:70329378-70329397 MS.gene002517:intron 45.0%
! TCCTTGATCTGCAGTTTGCT+AGG - chr2.1:70328335-70328354 MS.gene002517:CDS 45.0%
!! AATCATGCTGATGTTGGCTC+TGG + chr2.1:70329224-70329243 None:intergenic 45.0%
!! GCTGTAAGAAAGCATCACAC+AGG - chr2.1:70328263-70328282 MS.gene002517:CDS 45.0%
!! GTTGCCTGATTATAATGGGC+GGG - chr2.1:70329932-70329951 MS.gene002517:CDS 45.0%
!! TCTCGAAGAGCATTTGAGAG+TGG - chr2.1:70329891-70329910 MS.gene002517:CDS 45.0%
!! TGATCTGCAGTTTGCTAGGA+AGG - chr2.1:70328339-70328358 MS.gene002517:CDS 45.0%
!! TTGCCTGATTATAATGGGCG+GGG - chr2.1:70329933-70329952 MS.gene002517:CDS 45.0%
ACACAAGTTCAACCCTTCCG+CGG + chr2.1:70329514-70329533 None:intergenic 50.0%
ACCTAATGCCAAGGCTGACA+TGG - chr2.1:70330244-70330263 MS.gene002517:CDS 50.0%
ACCTGCTGCAAAGAAGAAGC+AGG + chr2.1:70328451-70328470 None:intergenic 50.0%
AGGAGAAGGCTCATTGAGCT+CGG + chr2.1:70328431-70328450 None:intergenic 50.0%
AGGGCAGATGCTTGGTAGAT+AGG + chr2.1:70329439-70329458 None:intergenic 50.0%
CAGAGCCAAACCTAATGCCA+AGG - chr2.1:70330235-70330254 MS.gene002517:CDS 50.0%
CATCCCCGCCCATTATAATC+AGG + chr2.1:70329939-70329958 None:intergenic 50.0%
CCTAATGCCAAGGCTGACAT+GGG - chr2.1:70330245-70330264 MS.gene002517:CDS 50.0%
GTCAGCCTTGGCATTAGGTT+TGG + chr2.1:70330243-70330262 None:intergenic 50.0%
TCCTGCTTCTTCTTTGCAGC+AGG - chr2.1:70328447-70328466 MS.gene002517:CDS 50.0%
TCTCGAGGGGGAGACTATTA+GGG - chr2.1:70329848-70329867 MS.gene002517:CDS 50.0%
TTCACCAGATGCTTCGTGAG+TGG - chr2.1:70328401-70328420 MS.gene002517:CDS 50.0%
TTCTCGAGGGGGAGACTATT+AGG - chr2.1:70329847-70329866 MS.gene002517:CDS 50.0%
!! AGGCACAGCTAATGGACTAG+TGG + chr2.1:70328679-70328698 None:intergenic 50.0%
!! CTGATTATAATGGGCGGGGA+TGG - chr2.1:70329937-70329956 MS.gene002517:CDS 50.0%
AACACCACCCATGTCAGCCT+TGG + chr2.1:70330255-70330274 None:intergenic 55.0%
AAGTCTCCGTGGAATGCACC+TGG - chr2.1:70329714-70329733 MS.gene002517:intron 55.0%
AATGCCAAGGCTGACATGGG+TGG - chr2.1:70330248-70330267 MS.gene002517:CDS 55.0%
ACTGTTCAAGACCCGCGGAA+GGG - chr2.1:70329499-70329518 MS.gene002517:intron 55.0%
AGGGGGAGACTATTAGGGAG+TGG - chr2.1:70329853-70329872 MS.gene002517:CDS 55.0%
ATAGCCCCAGTTCTACGACC+TGG - chr2.1:70329591-70329610 MS.gene002517:intron 55.0%
ATTCCCCCAGGTCGTAGAAC+TGG + chr2.1:70329600-70329619 None:intergenic 55.0%
CACAAGTTCAACCCTTCCGC+GGG + chr2.1:70329513-70329532 None:intergenic 55.0%
CCAGATGCTTCGTGAGTGGA+AGG - chr2.1:70328405-70328424 MS.gene002517:CDS 55.0%
CCCATGTCAGCCTTGGCATT+AGG + chr2.1:70330248-70330267 None:intergenic 55.0%
CTAGGAAAGCGGAAGGAGGA+GGG + chr2.1:70329458-70329477 None:intergenic 55.0%
GAACCTAGGAAAGCGGAAGG+AGG + chr2.1:70329462-70329481 None:intergenic 55.0%
GAAGCGCGACATCCACACTA+CGG + chr2.1:70328369-70328388 None:intergenic 55.0%
GCTACTGCAGCTCTGTGAAG+AGG - chr2.1:70328644-70328663 MS.gene002517:CDS 55.0%
TAGCCCCAGTTCTACGACCT+GGG - chr2.1:70329592-70329611 MS.gene002517:intron 55.0%
TAGGAAGGAGAGCCGTAGTG+TGG - chr2.1:70328354-70328373 MS.gene002517:CDS 55.0%
TGTGGATGTCGCGCTTCTTG+AGG - chr2.1:70328372-70328391 MS.gene002517:CDS 55.0%
TTCCCCCAGGTCGTAGAACT+GGG + chr2.1:70329599-70329618 None:intergenic 55.0%
TTGGGACTGTTCAAGACCCG+CGG - chr2.1:70329494-70329513 MS.gene002517:intron 55.0%
! CAGAGGCACAGGCTTTAGTG+AGG - chr2.1:70329389-70329408 MS.gene002517:intron 55.0%
! CCTTCCACTCACGAAGCATC+TGG + chr2.1:70328408-70328427 None:intergenic 55.0%
! GGCACAGGCTTTAGTGAGGA+TGG - chr2.1:70329393-70329412 MS.gene002517:intron 55.0%
AGCCCCAGTTCTACGACCTG+GGG - chr2.1:70329593-70329612 MS.gene002517:intron 60.0%
CATCCCAGAATGCGAGGGAG+CGG - chr2.1:70329683-70329702 MS.gene002517:intron 60.0%
CCTAGGAAAGCGGAAGGAGG+AGG + chr2.1:70329459-70329478 None:intergenic 60.0%
CCTCCTCCTTCCGCTTTCCT+AGG - chr2.1:70329456-70329475 MS.gene002517:intron 60.0%
CGGCAACTGCAAAGTCTCCG+TGG - chr2.1:70329703-70329722 MS.gene002517:intron 60.0%
CGTAGAACTGGGGCTATGCC+TGG + chr2.1:70329588-70329607 None:intergenic 60.0%
GAAGGAGGAGGGCAGATGCT+TGG + chr2.1:70329447-70329466 None:intergenic 60.0%
GACTGTTCAAGACCCGCGGA+AGG - chr2.1:70329498-70329517 MS.gene002517:intron 60.0%
GTCGGCATCCCAGAATGCGA+GGG - chr2.1:70329678-70329697 MS.gene002517:intron 60.0%
TCCCCCAGGTCGTAGAACTG+GGG + chr2.1:70329598-70329617 None:intergenic 60.0%
TGTCGGCATCCCAGAATGCG+AGG - chr2.1:70329677-70329696 MS.gene002517:intron 60.0%
TTGCCGCTCCCTCGCATTCT+GGG + chr2.1:70329689-70329708 None:intergenic 60.0%
GCCCCAGTTCTACGACCTGG+GGG - chr2.1:70329594-70329613 MS.gene002517:intron 65.0%
GTTGCCGCTCCCTCGCATTC+TGG + chr2.1:70329690-70329709 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 70328247 70330343 70328247 ID=MS.gene002517
chr2.1 mRNA 70328247 70330343 70328247 ID=MS.gene002517.t1;Parent=MS.gene002517
chr2.1 exon 70330122 70330343 70330122 ID=MS.gene002517.t1.exon1;Parent=MS.gene002517.t1
chr2.1 CDS 70330122 70330343 70330122 ID=cds.MS.gene002517.t1;Parent=MS.gene002517.t1
chr2.1 exon 70329835 70329984 70329835 ID=MS.gene002517.t1.exon2;Parent=MS.gene002517.t1
chr2.1 CDS 70329835 70329984 70329835 ID=cds.MS.gene002517.t1;Parent=MS.gene002517.t1
chr2.1 exon 70328855 70329365 70328855 ID=MS.gene002517.t1.exon3;Parent=MS.gene002517.t1
chr2.1 CDS 70328855 70329365 70328855 ID=cds.MS.gene002517.t1;Parent=MS.gene002517.t1
chr2.1 exon 70328247 70328761 70328247 ID=MS.gene002517.t1.exon4;Parent=MS.gene002517.t1
chr2.1 CDS 70328247 70328761 70328247 ID=cds.MS.gene002517.t1;Parent=MS.gene002517.t1
Gene Sequence

>MS.gene002517

ATGATTTCCAAGGGTAGCTGTAAGAAAGCATCACACAGGCTTATCAAGGACAAGGCAAAGAACCATGTAGATGATCTGCATGTGATGTTCCTTGATCTGCAGTTTGCTAGGAAGGAGAGCCGTAGTGTGGATGTCGCGCTTCTTGAGGAGCAAGTTCACCAGATGCTTCGTGAGTGGAAGGCCGAGCTCAATGAGCCTTCTCCTGCTTCTTCTTTGCAGCAGGTAAGCTCAGAATAGTTCTCCTTAACTTTAATATGCAGATTTAATTGTGGATTTGTGATGGACACATGTAAACTTGAATGGCAATATTTGTGGCAAAAACAATGATTTGAGTTTGCCATGTTTATGGGTGATGATAGGGTGGCAGTCTTCGATCATTCTCGACCGATATATGTAGGCTACTGCAGCTCTGTGAAGAGGAAGATGATGCCACTAGTCCATTAGCTGTGCCTAAGCATGAACCTAATGATCAGAATATGCAGACTGAAGCTAAGATGACGTTTCAAGAAGTCTGTTCCTTTCTCCTCTTCAGCGATTATTCATACATCTTTGTGATTTGATCATAACTGGCATAGTTTTTATTTTTTCTGTAGTATTGGCAGTCTAAAATATTGATTTGAAGCTTCTTTTTCTTTATTGCATTATTTTAATTCATATACTAGAGTAAGGGATGCTTTGGAATGTATGATATCTTCTTTTAATGCAAACAGAAAAATATTATAAAGTGTTGTATTGTTTTTTATATGTGGTAGCCTTTTCTCTAAAAAATAAAATTTAATCTTTTATTTTCAATTGACAGAAATGGAGAGCAAGGATTTGATGGTTCAGTCTTTCCTTAGTTAATACCAATTTTCTCACCAACAACTCAAAGCCCAATTTGTTCTTATGCATCATAGTTTTAATCATTATTTCAATGCTTGTCAATATTTTGATCTCACATATGATTGTAACAAGTTTCTTGGTTTAAATTTAAACCAGAGCCAACATCAGCATGATTCCCTATTAATGGATGAGTACATACACTCCATCTTAGGAGCTCCCAGTAAATCAACTAACAACTCGGATAGGCGGCCTAATTTTGAATCAAAATGTCGTCAATTTGATTTAAATGAAGATTTTAATCACAACTGTTATACTGCTTTCAGAGGCACAGGCTTTAGTGAGGATGGTGTTGTTCCTTATATACCTACCTATCTACCAAGCATCTGCCCTCCTCCTTCCGCTTTCCTAGGTTCAAAATGTGCACTTTGGGACTGTTCAAGACCCGCGGAAGGGTTGAACTTGTGTCAAGACTATTGCAGTAGTTTTCATGCTGCTCTTGCTTTAAATGAAGGACAACCAGGCATAGCCCCAGTTCTACGACCTGGGGGAATTGAAGTTAAGGATAATTTACTGTTGGCTGCTCTTAGTGCAAAGACAGAAGGAAAAGATGTCGGCATCCCAGAATGCGAGGGAGCGGCAACTGCAAAGTCTCCGTGGAATGCACCTGGTATTAGAATGTAGAATGCTGAGTTTACCTATTAAACTATTTATGTGTTTATTGAGTATAAAAGTTGAGAAGAGTATTCTTTTTTTGTTGCAGAGCTCTTTGATCTTTTTGTTCTCGAGGGGGAGACTATTAGGGAGTGGCTTTTCTTTGATAAGTCTCGAAGAGCATTTGAGAGTGGAAACAGAAAGCAAAGGTCGTTGCCTGATTATAATGGGCGGGGATGGCATGAATCCAGAAAGCAAGTGATGAAAGAGTTTGGAGGGCTGAAGAGATCCTACTACATGGATCCACAACCACTAAACCATTTTGAGTGGCATCTTTATGAGTATGAAATTAGCAAGTGTGATGCATGTGTGTTATATCGGTTGGAACTAAAGCTCGTTAATGGGAAGAAGAGCTCCAGGACAAAGATAACAATTGATTCAGTTACTGATCTTCAGAAGCGGATAAGAAGACTCTCTGCCGAGTTTCCCTATGATAACAGACATTCTGCTAAGGGCAGAGCCAAACCTAATGCCAAGGCTGACATGGGTGGTGTTTATACAGCTTCAAACAATGCACCATCACCATATGACTATCTTGTTGAGAGCACAATCGCTATTTTGTGA
Protein sequence

>MS.gene002517.t1

MISKGSCKKASHRLIKDKAKNHVDDLHVMFLDLQFARKESRSVDVALLEEQVHQMLREWKAELNEPSPASSLQQGGSLRSFSTDICRLLQLCEEEDDATSPLAVPKHEPNDQNMQTEAKMTFQESQHQHDSLLMDEYIHSILGAPSKSTNNSDRRPNFESKCRQFDLNEDFNHNCYTAFRGTGFSEDGVVPYIPTYLPSICPPPSAFLGSKCALWDCSRPAEGLNLCQDYCSSFHAALALNEGQPGIAPVLRPGGIEVKDNLLLAALSAKTEGKDVGIPECEGAATAKSPWNAPELFDLFVLEGETIREWLFFDKSRRAFESGNRKQRSLPDYNGRGWHESRKQVMKEFGGLKRSYYMDPQPLNHFEWHLYEYEISKCDACVLYRLELKLVNGKKSSRTKITIDSVTDLQKRIRRLSAEFPYDNRHSAKGRAKPNAKADMGGVYTASNNAPSPYDYLVESTIAIL