AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene002526

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002526.t1 MTR_2g015000 97.356 416 11 0 1 416 1 416 0.0 829
MS.gene002526.t1 MTR_7g025010 45.434 438 172 18 1 405 1 404 1.93e-87 277
MS.gene002526.t1 MTR_4g088070 39.948 388 178 13 42 405 16 372 7.08e-62 206
MS.gene002526.t1 MTR_4g088080 39.298 285 124 13 46 314 31 282 4.76e-34 129
MS.gene002526.t1 MTR_6g027390 66.667 60 20 0 47 106 24 83 6.11e-23 98.6
MS.gene002526.t1 MTR_7g086940 59.341 91 36 1 39 129 13 102 3.40e-22 95.9
MS.gene002526.t1 MTR_7g086940 46.269 67 36 0 341 407 201 267 1.52e-14 73.9
MS.gene002526.t1 MTR_4g084550 37.805 82 51 0 50 131 59 140 5.48e-18 81.6
MS.gene002526.t1 MTR_4g088065 42.400 125 52 3 64 188 134 238 7.69e-16 77.0
MS.gene002526.t1 MTR_8g107210 35.238 105 68 0 55 159 96 200 1.64e-15 77.8
MS.gene002526.t1 MTR_3g462480 42.708 96 46 1 311 406 4 90 4.17e-15 70.9
MS.gene002526.t1 MTR_1g115315 44.615 65 36 0 53 117 83 147 6.07e-15 74.7
MS.gene002526.t1 MTR_5g081990 45.312 64 35 0 55 118 33 96 6.27e-15 73.2
MS.gene002526.t1 MTR_1g115315 44.615 65 36 0 53 117 83 147 6.62e-15 74.7
MS.gene002526.t1 MTR_3g115620 37.363 91 56 1 24 114 52 141 6.68e-15 73.9
MS.gene002526.t1 MTR_7g060630 43.836 73 41 0 49 121 2 74 1.89e-14 72.4
MS.gene002526.t1 MTR_5g021930 42.667 75 42 1 49 122 24 98 4.02e-14 73.2
MS.gene002526.t1 MTR_1g019130 50.000 60 30 0 55 114 90 149 4.12e-14 71.6
MS.gene002526.t1 MTR_4g063735 50.000 60 30 0 55 114 25 84 6.82e-14 71.6
MS.gene002526.t1 MTR_1g019130 50.000 60 30 0 55 114 90 149 1.05e-13 70.9
MS.gene002526.t1 MTR_7g023810 42.424 66 38 0 49 114 62 127 2.03e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene002526.t1 AT5G45980 36.451 417 162 11 1 406 1 325 3.74e-74 234
MS.gene002526.t1 AT2G33880 85.714 126 14 3 1 124 1 124 2.13e-68 221
MS.gene002526.t1 AT2G33880 85.714 126 14 3 1 124 1 124 2.57e-68 221
MS.gene002526.t1 AT3G03660 64.286 70 24 1 38 106 15 84 1.58e-23 99.8
MS.gene002526.t1 AT3G03660 52.632 57 27 0 350 406 208 264 4.86e-13 69.3
MS.gene002526.t1 AT3G03660 64.286 70 24 1 38 106 15 84 2.54e-23 99.8
MS.gene002526.t1 AT3G03660 52.632 57 27 0 350 406 237 293 7.42e-13 69.3
MS.gene002526.t1 AT3G03660 64.286 70 24 1 38 106 15 84 4.44e-23 99.0
MS.gene002526.t1 AT3G03660 53.571 56 26 0 351 406 209 264 1.58e-12 68.2
MS.gene002526.t1 AT3G03660 64.286 70 24 1 38 106 15 84 6.64e-23 99.0
MS.gene002526.t1 AT3G03660 53.571 56 26 0 351 406 238 293 2.15e-12 68.2
MS.gene002526.t1 AT5G17810 67.797 59 18 1 49 106 14 72 3.54e-21 92.8
MS.gene002526.t1 AT5G17810 50.877 57 28 0 350 406 207 263 1.86e-13 70.5
MS.gene002526.t1 AT5G17810 67.797 59 18 1 49 106 14 72 3.64e-21 92.8
MS.gene002526.t1 AT5G17810 50.877 57 28 0 350 406 208 264 1.82e-13 70.5
MS.gene002526.t1 AT5G17810 67.797 59 18 1 49 106 14 72 3.64e-21 92.8
MS.gene002526.t1 AT5G17810 50.877 57 28 0 350 406 208 264 1.82e-13 70.5
MS.gene002526.t1 AT5G05770 34.066 91 60 0 55 145 29 119 1.12e-15 73.6
MS.gene002526.t1 AT3G18010 48.387 62 32 0 55 116 76 137 5.74e-15 76.3
MS.gene002526.t1 AT2G28610 48.529 68 35 0 49 116 2 69 8.33e-15 74.3
MS.gene002526.t1 AT1G20700 46.774 62 33 0 53 114 31 92 1.24e-14 71.2
MS.gene002526.t1 AT3G11260 45.312 64 35 0 55 118 24 87 1.44e-14 72.0
MS.gene002526.t1 AT1G20700 46.774 62 33 0 53 114 93 154 2.55e-14 72.0
MS.gene002526.t1 AT1G20700 46.774 62 33 0 53 114 93 154 2.60e-14 72.0
MS.gene002526.t1 AT1G46480 50.000 60 30 0 55 114 90 149 3.75e-14 71.2
MS.gene002526.t1 AT4G35550 45.161 62 34 0 53 114 97 158 1.36e-13 70.9
MS.gene002526.t1 AT1G46480 50.000 60 30 0 55 114 90 149 1.79e-13 70.5
MS.gene002526.t1 AT2G01500 50.909 55 27 0 55 109 61 115 5.95e-13 69.3
MS.gene002526.t1 AT5G46010 47.619 63 32 1 51 112 50 112 1.13e-12 64.7
MS.gene002526.t1 AT5G59340 43.333 60 34 0 55 114 14 73 1.31e-12 68.2
MS.gene002526.t1 AT2G17950 46.032 63 33 1 55 116 38 100 2.25e-12 67.8

Find 100 sgRNAs with CRISPR-Local

Find 149 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGATTCTGATTCTGATTC+TGG 0.081403 2.1:+70247543 None:intergenic
GAAATTTGAGGAAGTGAATT+AGG 0.138466 2.1:+70247500 None:intergenic
TGCTTCTTGATTTGCGGTTT+TGG 0.159712 2.1:+70247609 None:intergenic
TCTGATTCTTGTTCTGGTTT+TGG 0.210118 2.1:+70247567 None:intergenic
GTGGCCTTTGAGGTTGCTTC+TGG 0.210381 2.1:-70246258 MS.gene002526:CDS
TCTGGTTCTGATTCTTGTTC+TGG 0.215529 2.1:+70247561 None:intergenic
CTTGAAGCTATCTTCAATTC+TGG 0.223036 2.1:-70247725 MS.gene002526:CDS
TTTAATGTTCGTGCGGCATT+TGG 0.234206 2.1:-70246231 MS.gene002526:CDS
ATGCGGATTTGTTCTGGTTT+TGG 0.247339 2.1:+70247749 None:intergenic
TTGGCAGGTATTAATTCTTT+TGG 0.251759 2.1:+70247431 None:intergenic
TTAATGTTCGTGCGGCATTT+GGG 0.270781 2.1:-70246230 MS.gene002526:CDS
CCTTGTGATGTAACATTGTT+AGG 0.298506 2.1:+70247218 None:intergenic
TTTGGATTCCACCTTGGCTT+TGG 0.309423 2.1:+70247767 None:intergenic
CCATTGGTGATGAGTGTTGC+AGG 0.323664 2.1:+70248024 None:intergenic
TCAAGTATGCGGATTTGTTC+TGG 0.334510 2.1:+70247743 None:intergenic
AATGCCGCACGAACATTAAA+AGG 0.340006 2.1:+70246234 None:intergenic
GGCACAACATCATTGATGTT+AGG 0.346091 2.1:+70247389 None:intergenic
GCTGAAAATATGTTTGGTTT+TGG 0.348978 2.1:+70247342 None:intergenic
ATCTTCCTTATCTCTTCTCT+TGG 0.351542 2.1:+70247689 None:intergenic
CTGTTCTTGATCTTTCTTTG+AGG 0.356529 2.1:+70247070 None:intergenic
ACAGAAGCAGTTGGAGAATT+AGG 0.358074 2.1:+70247368 None:intergenic
TTTGAGGCTGAAAATATGTT+TGG 0.371353 2.1:+70247336 None:intergenic
TTCCACCTTGGCTTTGGCTC+AGG 0.373630 2.1:+70247773 None:intergenic
TTCCTTATCTCTTCTCTTGG+TGG 0.377528 2.1:+70247692 None:intergenic
AAATTTGAGGAAGTGAATTA+GGG 0.386654 2.1:+70247501 None:intergenic
CCTTGCACTTGAGAAATGAT+AGG 0.417012 2.1:+70246927 None:intergenic
GTAGGATGTAAAACAGTAGT+AGG 0.421080 2.1:+70246978 None:intergenic
CCAGAATCAGAATCAGAATC+AGG 0.428113 2.1:-70247543 MS.gene002526:CDS
TATGCTTGCTTCTTGATTTG+CGG 0.428894 2.1:+70247603 None:intergenic
GCACAACATCATTGATGTTA+GGG 0.443675 2.1:+70247390 None:intergenic
TATCAATGATGTGGCCTTTG+AGG 0.444367 2.1:-70246268 MS.gene002526:CDS
AGAGCACAGTTGCAAGAGTA+TGG 0.448490 2.1:-70247665 MS.gene002526:CDS
TAGTGCAGTTACCAGTGTTA+TGG 0.449318 2.1:+70247138 None:intergenic
GGCTTAGACTTAAACATGCT+TGG 0.459758 2.1:+70248046 None:intergenic
TTACTTGTGGTGGTGGAAGA+AGG 0.462598 2.1:+70247303 None:intergenic
TTCTTCCCAGTGCAACATCA+TGG 0.463536 2.1:-70247257 MS.gene002526:CDS
CTCAAAGAAAGATCAAGAAC+AGG 0.467360 2.1:-70247069 MS.gene002526:CDS
AGATAATTACCTGAGGAGTA+AGG 0.470017 2.1:+70247953 None:intergenic
ATCTCAGGCATTGGTGAACC+AGG 0.475607 2.1:-70246315 MS.gene002526:intron
GCTGTTCTTGTACATCAAAC+TGG 0.484452 2.1:-70246201 MS.gene002526:CDS
GAAAATAACATGCACCATGC+TGG 0.488247 2.1:+70246133 None:intergenic
CCTAACAATGTTACATCACA+AGG 0.488908 2.1:-70247218 MS.gene002526:CDS
CCAAGGGAAAATACTTTGGC+AGG 0.496441 2.1:+70247416 None:intergenic
CCTTGTCCATGATGTTGCAC+TGG 0.500743 2.1:+70247251 None:intergenic
TGCTGACCATGTGGTTGAGC+AGG 0.506086 2.1:+70247164 None:intergenic
GAAACCAAGGGAAAATACTT+TGG 0.514758 2.1:+70247412 None:intergenic
GATAATTACCTGAGGAGTAA+GGG 0.515469 2.1:+70247954 None:intergenic
CACAGAACCCCTTACTCCTC+AGG 0.516334 2.1:-70247962 MS.gene002526:intron
AGTGAAGAGTGACACCATAC+TGG 0.516391 2.1:+70246160 None:intergenic
TGTTCATGATCTCACTAAGC+AGG 0.516785 2.1:+70247108 None:intergenic
GTGATGGTGGAGGAAGTAGT+AGG 0.517817 2.1:+70246960 None:intergenic
TAATGTTCGTGCGGCATTTG+GGG 0.529446 2.1:-70246229 MS.gene002526:CDS
AAGAAGAAAGTCTCAGTAGC+TGG 0.533457 2.1:+70247275 None:intergenic
GGTACAGTAGCAGAAATTTG+AGG 0.535619 2.1:+70247488 None:intergenic
GGTGCAGTTACTTGTGGTGG+TGG 0.536505 2.1:+70247296 None:intergenic
ATCCACCAAGAGAAGAGATA+AGG 0.537259 2.1:-70247694 MS.gene002526:CDS
TTGCAAGAGTATGGTCAAGT+TGG 0.545746 2.1:-70247656 MS.gene002526:CDS
TACTTGTGGTGGTGGAAGAA+GGG 0.555078 2.1:+70247304 None:intergenic
AGCTATCTTCAATTCTGGAA+TGG 0.556620 2.1:-70247720 MS.gene002526:CDS
GCAAACAGATGTTCAGATGT+GGG 0.556845 2.1:+70247015 None:intergenic
GAGGAAGATGAAGAAGATGA+AGG 0.560821 2.1:+70247467 None:intergenic
CCTGTGTTAACAAACCAGTA+TGG 0.574262 2.1:-70246174 MS.gene002526:CDS
AGGTGATTGAACAGTGATGG+TGG 0.575194 2.1:+70246947 None:intergenic
GGCAAACAGATGTTCAGATG+TGG 0.578783 2.1:+70247014 None:intergenic
AACAGTAGTAGGAGTTGTGG+CGG 0.580421 2.1:+70246989 None:intergenic
CTTGTCCATGATGTTGCACT+GGG 0.582831 2.1:+70247252 None:intergenic
CATTGATGTTAGGGAAACCA+AGG 0.582855 2.1:+70247399 None:intergenic
ACTCTTCACTCACTCCAGCA+TGG 0.587267 2.1:-70246147 MS.gene002526:CDS
CATTGGTGATGAGTGTTGCA+GGG 0.588949 2.1:+70248025 None:intergenic
GTAGCTGGTGCAGTTACTTG+TGG 0.590783 2.1:+70247290 None:intergenic
AGTTGATGTCATGCTGCCAT+TGG 0.593818 2.1:+70248008 None:intergenic
GAAGTCCTGAGCCAAAGCCA+AGG 0.598879 2.1:-70247778 MS.gene002526:CDS
AAACAGATGTTCAGATGTGG+GGG 0.599793 2.1:+70247017 None:intergenic
CCTATCATTTCTCAAGTGCA+AGG 0.604894 2.1:-70246927 MS.gene002526:intron
TGAAGATAGCTTCAAGTATG+CGG 0.609197 2.1:+70247732 None:intergenic
ATAATTACCTGAGGAGTAAG+GGG 0.610710 2.1:+70247955 None:intergenic
CAAACAGATGTTCAGATGTG+GGG 0.614296 2.1:+70247016 None:intergenic
TAAAACAGTAGTAGGAGTTG+TGG 0.614312 2.1:+70246986 None:intergenic
CCATACTGGTTTGTTAACAC+AGG 0.617631 2.1:+70246174 None:intergenic
GCTGGTGCAGTTACTTGTGG+TGG 0.617972 2.1:+70247293 None:intergenic
AGGTCCAGAAGCAACCTCAA+AGG 0.623758 2.1:+70246254 None:intergenic
GTAGTAGGAGTTGTGGCGGG+TGG 0.627094 2.1:+70246993 None:intergenic
ATGGTTCCTGCTCAACCACA+TGG 0.630924 2.1:-70247170 MS.gene002526:CDS
TGGCTTCATCAAATAGACAC+TGG 0.631121 2.1:-70248069 MS.gene002526:CDS
CCAGTGCAACATCATGGACA+AGG 0.635853 2.1:-70247251 MS.gene002526:CDS
GATAGGTGATTGAACAGTGA+TGG 0.637240 2.1:+70246944 None:intergenic
ACAGTAGTAGGAGTTGTGGC+GGG 0.646909 2.1:+70246990 None:intergenic
GTCCTGAGCCAAAGCCAAGG+TGG 0.648407 2.1:-70247775 MS.gene002526:CDS
CCTGCAACACTCATCACCAA+TGG 0.655110 2.1:-70248024 MS.gene002526:CDS
ATTGATGTTAGGGAAACCAA+GGG 0.665295 2.1:+70247400 None:intergenic
CTAACAATGTTACATCACAA+GGG 0.668058 2.1:-70247217 MS.gene002526:CDS
GGTCAGCAACACCATAACAC+TGG 0.670176 2.1:-70247149 MS.gene002526:CDS
TTATGGTGTTGCTGACCATG+TGG 0.673188 2.1:+70247155 None:intergenic
AGTGAGATCATGAACAACCA+TGG 0.690840 2.1:-70247101 MS.gene002526:CDS
TGGTTGAGCAGGAACCATGT+TGG 0.698137 2.1:+70247175 None:intergenic
TCTTTGAGGAATTAACACCA+TGG 0.698293 2.1:+70247084 None:intergenic
AGTGGTGTTTATCAATGATG+TGG 0.701398 2.1:-70246277 MS.gene002526:CDS
TGATTGAACAGTGATGGTGG+AGG 0.713068 2.1:+70246950 None:intergenic
AACAGATGTTCAGATGTGGG+GGG 0.713218 2.1:+70247018 None:intergenic
AGGTGTTGCTGCAAAATCAG+TGG 0.717993 2.1:-70246295 MS.gene002526:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAGAAACTAAATAATTT+GGG + chr2.1:70247268-70247287 None:intergenic 10.0%
!! AAGTATATAGATGAAATAAT+AGG + chr2.1:70247493-70247512 None:intergenic 15.0%
!! GTTAAAGAAACTAAATAATT+TGG + chr2.1:70247269-70247288 None:intergenic 15.0%
!! TAAAGAAACTAAATAATTTG+GGG + chr2.1:70247267-70247286 None:intergenic 15.0%
!! TATGAAAAATAAATTAGAGA+AGG + chr2.1:70248030-70248049 None:intergenic 15.0%
!! AATTTAGAGATGAAAATATG+TGG + chr2.1:70247301-70247320 None:intergenic 20.0%
!! TTGTTACAAATTAGACATTA+GGG + chr2.1:70246230-70246249 None:intergenic 20.0%
!!! CATAGTTTTTGAGATAAAAA+TGG - chr2.1:70247545-70247564 MS.gene002526:CDS 20.0%
!!! TAAAAATGGTAATGTATGTA+TGG - chr2.1:70247559-70247578 MS.gene002526:CDS 20.0%
!!! TTTAGTATTAGTTTGACTTT+TGG - chr2.1:70247686-70247705 MS.gene002526:CDS 20.0%
! AAAATGTAGATAATTACCTG+AGG + chr2.1:70246134-70246153 None:intergenic 25.0%
! AAATTTGAGGAAGTGAATTA+GGG + chr2.1:70246579-70246598 None:intergenic 25.0%
! ATCTCAAAAACTATGTTCTT+AGG + chr2.1:70247540-70247559 None:intergenic 25.0%
! CTTGTTACAAATTAGACATT+AGG + chr2.1:70246231-70246250 None:intergenic 25.0%
!!! ATATTTTGTTTTGCAGATGT+AGG - chr2.1:70248049-70248068 MS.gene002526:CDS 25.0%
!!! TATTTTGTTTTGCAGATGTA+GGG - chr2.1:70248050-70248069 MS.gene002526:CDS 25.0%
ACTATGTTCTTAGGTTCTAT+AGG + chr2.1:70247531-70247550 None:intergenic 30.0%
AGATTAATGTGAATAAGACG+TGG - chr2.1:70247605-70247624 MS.gene002526:CDS 30.0%
ATAAACTAGTCAAGATCTTG+TGG + chr2.1:70247445-70247464 None:intergenic 30.0%
CTAACAATGTTACATCACAA+GGG - chr2.1:70246860-70246879 MS.gene002526:intron 30.0%
GAAATTTGAGGAAGTGAATT+AGG + chr2.1:70246580-70246599 None:intergenic 30.0%
TATGGAAAAGAAGAGAAAGT+GGG - chr2.1:70247577-70247596 MS.gene002526:CDS 30.0%
TTTCTTCTTCTTGTGGAAAT+AGG - chr2.1:70246265-70246284 MS.gene002526:CDS 30.0%
! AGACATTTTTCTTCTTCTTG+TGG - chr2.1:70246258-70246277 MS.gene002526:CDS 30.0%
! GTGATGCTAATGTTTTCTAT+TGG - chr2.1:70246443-70246462 MS.gene002526:intron 30.0%
! TTGGCAGGTATTAATTCTTT+TGG + chr2.1:70246649-70246668 None:intergenic 30.0%
! TTTGAGGCTGAAAATATGTT+TGG + chr2.1:70246744-70246763 None:intergenic 30.0%
!! ACTTTTGGTAGCTATAGTTA+AGG - chr2.1:70247701-70247720 MS.gene002526:CDS 30.0%
!! GCTGAAAATATGTTTGGTTT+TGG + chr2.1:70246738-70246757 None:intergenic 30.0%
!!! ATTTTGTTTTGCAGATGTAG+GGG - chr2.1:70248051-70248070 MS.gene002526:CDS 30.0%
AGATAATTACCTGAGGAGTA+AGG + chr2.1:70246127-70246146 None:intergenic 35.0%
AGTGGTGTTTATCAATGATG+TGG - chr2.1:70247800-70247819 MS.gene002526:intron 35.0%
ATAATTACCTGAGGAGTAAG+GGG + chr2.1:70246125-70246144 None:intergenic 35.0%
ATCTTCCTTATCTCTTCTCT+TGG + chr2.1:70246391-70246410 None:intergenic 35.0%
ATGTTCTTAGGTTCTATAGG+CGG + chr2.1:70247528-70247547 None:intergenic 35.0%
ATTGATGTTAGGGAAACCAA+GGG + chr2.1:70246680-70246699 None:intergenic 35.0%
CCTAACAATGTTACATCACA+AGG - chr2.1:70246859-70246878 MS.gene002526:intron 35.0%
CCTTGTGATGTAACATTGTT+AGG + chr2.1:70246862-70246881 None:intergenic 35.0%
CTCAAAGAAAGATCAAGAAC+AGG - chr2.1:70247008-70247027 MS.gene002526:CDS 35.0%
CTGTTCTTGATCTTTCTTTG+AGG + chr2.1:70247010-70247029 None:intergenic 35.0%
CTTGAAGCTATCTTCAATTC+TGG - chr2.1:70246352-70246371 MS.gene002526:intron 35.0%
GAAACCAAGGGAAAATACTT+TGG + chr2.1:70246668-70246687 None:intergenic 35.0%
GAAATGATTGAGTGTATCTC+AGG - chr2.1:70247747-70247766 MS.gene002526:CDS 35.0%
GATAATTACCTGAGGAGTAA+GGG + chr2.1:70246126-70246145 None:intergenic 35.0%
GCACAACATCATTGATGTTA+GGG + chr2.1:70246690-70246709 None:intergenic 35.0%
GTAGGATGTAAAACAGTAGT+AGG + chr2.1:70247102-70247121 None:intergenic 35.0%
GTATGGAAAAGAAGAGAAAG+TGG - chr2.1:70247576-70247595 MS.gene002526:CDS 35.0%
TAAAACAGTAGTAGGAGTTG+TGG + chr2.1:70247094-70247113 None:intergenic 35.0%
TGAAGATAGCTTCAAGTATG+CGG + chr2.1:70246348-70246367 None:intergenic 35.0%
! AGAAGGGTAGTTACATTTTG+AGG + chr2.1:70246760-70246779 None:intergenic 35.0%
! AGCTATCTTCAATTCTGGAA+TGG - chr2.1:70246357-70246376 MS.gene002526:intron 35.0%
! CTTTTCTGAACTTTCCAACA+TGG - chr2.1:70246888-70246907 MS.gene002526:intron 35.0%
! GCAGGTATTAATTCTTTTGG+TGG + chr2.1:70246646-70246665 None:intergenic 35.0%
! GTTAGTGTACCATGGTTTTT+GGG - chr2.1:70247627-70247646 MS.gene002526:CDS 35.0%
! TATGCTTGCTTCTTGATTTG+CGG + chr2.1:70246477-70246496 None:intergenic 35.0%
! TCTTTGAGGAATTAACACCA+TGG + chr2.1:70246996-70247015 None:intergenic 35.0%
!! GGCTCTATTGGCTTAAATAT+AGG - chr2.1:70247662-70247681 MS.gene002526:CDS 35.0%
!!! AGTGAATTAGGGTTTTGTTG+TGG + chr2.1:70246568-70246587 None:intergenic 35.0%
!!! TCTGATTCTTGTTCTGGTTT+TGG + chr2.1:70246513-70246532 None:intergenic 35.0%
AAACAGATGTTCAGATGTGG+GGG + chr2.1:70247063-70247082 None:intergenic 40.0%
AAGAAGAAAGTCTCAGTAGC+TGG + chr2.1:70246805-70246824 None:intergenic 40.0%
AATGCCGCACGAACATTAAA+AGG + chr2.1:70247846-70247865 None:intergenic 40.0%
ACAGAAGCAGTTGGAGAATT+AGG + chr2.1:70246712-70246731 None:intergenic 40.0%
AGATGTAGGGGAAAAAATGC+TGG - chr2.1:70248063-70248082 MS.gene002526:CDS 40.0%
AGTGAGATCATGAACAACCA+TGG - chr2.1:70246976-70246995 MS.gene002526:CDS 40.0%
ATCCACCAAGAGAAGAGATA+AGG - chr2.1:70246383-70246402 MS.gene002526:intron 40.0%
ATTGAGTGTATCTCAGGCAT+TGG - chr2.1:70247753-70247772 MS.gene002526:CDS 40.0%
CAAACAGATGTTCAGATGTG+GGG + chr2.1:70247064-70247083 None:intergenic 40.0%
CATTGATGTTAGGGAAACCA+AGG + chr2.1:70246681-70246700 None:intergenic 40.0%
CCAGAATCAGAATCAGAATC+AGG - chr2.1:70246534-70246553 MS.gene002526:intron 40.0%
CCTATCATTTCTCAAGTGCA+AGG - chr2.1:70247150-70247169 MS.gene002526:CDS 40.0%
CCTGATTCTGATTCTGATTC+TGG + chr2.1:70246537-70246556 None:intergenic 40.0%
CCTGTGTTAACAAACCAGTA+TGG - chr2.1:70247903-70247922 MS.gene002526:intron 40.0%
CCTTGCACTTGAGAAATGAT+AGG + chr2.1:70247153-70247172 None:intergenic 40.0%
GAAAATAACATGCACCATGC+TGG + chr2.1:70247947-70247966 None:intergenic 40.0%
GAGGAAGATGAAGAAGATGA+AGG + chr2.1:70246613-70246632 None:intergenic 40.0%
GATAGGTGATTGAACAGTGA+TGG + chr2.1:70247136-70247155 None:intergenic 40.0%
GCAAACAGATGTTCAGATGT+GGG + chr2.1:70247065-70247084 None:intergenic 40.0%
GCTGTTCTTGTACATCAAAC+TGG - chr2.1:70247876-70247895 MS.gene002526:intron 40.0%
GGCACAACATCATTGATGTT+AGG + chr2.1:70246691-70246710 None:intergenic 40.0%
GGCTTAGACTTAAACATGCT+TGG + chr2.1:70246034-70246053 None:intergenic 40.0%
GGTACAGTAGCAGAAATTTG+AGG + chr2.1:70246592-70246611 None:intergenic 40.0%
TAGTGCAGTTACCAGTGTTA+TGG + chr2.1:70246942-70246961 None:intergenic 40.0%
TATCAATGATGTGGCCTTTG+AGG - chr2.1:70247809-70247828 MS.gene002526:intron 40.0%
TGGCTTCATCAAATAGACAC+TGG - chr2.1:70246008-70246027 MS.gene002526:CDS 40.0%
TGTTCATGATCTCACTAAGC+AGG + chr2.1:70246972-70246991 None:intergenic 40.0%
TTAATGTTCGTGCGGCATTT+GGG - chr2.1:70247847-70247866 MS.gene002526:intron 40.0%
TTCCTTATCTCTTCTCTTGG+TGG + chr2.1:70246388-70246407 None:intergenic 40.0%
TTTAATGTTCGTGCGGCATT+TGG - chr2.1:70247846-70247865 MS.gene002526:intron 40.0%
! GGTTAGTGTACCATGGTTTT+TGG - chr2.1:70247626-70247645 MS.gene002526:CDS 40.0%
! TCAAGTATGCGGATTTGTTC+TGG + chr2.1:70246337-70246356 None:intergenic 40.0%
! TGGACCTTTTAATGTTCGTG+CGG - chr2.1:70247839-70247858 MS.gene002526:intron 40.0%
! TTGCAAGAGTATGGTCAAGT+TGG - chr2.1:70246421-70246440 MS.gene002526:intron 40.0%
! TTTTGGTGGAGATTTCTCAG+AGG + chr2.1:70246632-70246651 None:intergenic 40.0%
!! CCATACTGGTTTGTTAACAC+AGG + chr2.1:70247906-70247925 None:intergenic 40.0%
!! TCTGGTTCTGATTCTTGTTC+TGG + chr2.1:70246519-70246538 None:intergenic 40.0%
!!! ATGCGGATTTGTTCTGGTTT+TGG + chr2.1:70246331-70246350 None:intergenic 40.0%
!!! TCTTGTTCTGGTTTTGGAGA+TGG + chr2.1:70246507-70246526 None:intergenic 40.0%
!!! TGCTTCTTGATTTGCGGTTT+TGG + chr2.1:70246471-70246490 None:intergenic 40.0%
!!! TTTTGGTTCACAGAAGCAGT+TGG + chr2.1:70246721-70246740 None:intergenic 40.0%
AACAGATGTTCAGATGTGGG+GGG + chr2.1:70247062-70247081 None:intergenic 45.0%
AACAGTAGTAGGAGTTGTGG+CGG + chr2.1:70247091-70247110 None:intergenic 45.0%
AAGACGTGGTTAGTGTACCA+TGG - chr2.1:70247619-70247638 MS.gene002526:CDS 45.0%
AGAGCACAGTTGCAAGAGTA+TGG - chr2.1:70246412-70246431 MS.gene002526:intron 45.0%
AGGTGATTGAACAGTGATGG+TGG + chr2.1:70247133-70247152 None:intergenic 45.0%
AGTGAAGAGTGACACCATAC+TGG + chr2.1:70247920-70247939 None:intergenic 45.0%
CCAAGGGAAAATACTTTGGC+AGG + chr2.1:70246664-70246683 None:intergenic 45.0%
CTTGTCCATGATGTTGCACT+GGG + chr2.1:70246828-70246847 None:intergenic 45.0%
GGCAAACAGATGTTCAGATG+TGG + chr2.1:70247066-70247085 None:intergenic 45.0%
TAATGTTCGTGCGGCATTTG+GGG - chr2.1:70247848-70247867 MS.gene002526:intron 45.0%
TTCTTCCCAGTGCAACATCA+TGG - chr2.1:70246820-70246839 MS.gene002526:intron 45.0%
TTTGGATTCCACCTTGGCTT+TGG + chr2.1:70246313-70246332 None:intergenic 45.0%
! AGTTGATGTCATGCTGCCAT+TGG + chr2.1:70246072-70246091 None:intergenic 45.0%
!! ACTGATTTTGCAGCAACACC+TGG + chr2.1:70247783-70247802 None:intergenic 45.0%
!! AGGTGTTGCTGCAAAATCAG+TGG - chr2.1:70247782-70247801 MS.gene002526:CDS 45.0%
!! CATTGGTGATGAGTGTTGCA+GGG + chr2.1:70246055-70246074 None:intergenic 45.0%
!! CCTGCCAAAGTATTTTCCCT+TGG - chr2.1:70246661-70246680 MS.gene002526:intron 45.0%
!! TACTTGTGGTGGTGGAAGAA+GGG + chr2.1:70246776-70246795 None:intergenic 45.0%
!! TGATTGAACAGTGATGGTGG+AGG + chr2.1:70247130-70247149 None:intergenic 45.0%
!! TTACTTGTGGTGGTGGAAGA+AGG + chr2.1:70246777-70246796 None:intergenic 45.0%
!! TTATGGTGTTGCTGACCATG+TGG + chr2.1:70246925-70246944 None:intergenic 45.0%
!!! TCTGGTTTTGGATTCCACCT+TGG + chr2.1:70246319-70246338 None:intergenic 45.0%
!!! TATTTTTTTATTTTTCTTTT+AGG - chr2.1:70247462-70247481 MS.gene002526:CDS 5.0%
ACAGTAGTAGGAGTTGTGGC+GGG + chr2.1:70247090-70247109 None:intergenic 50.0%
ACTCTTCACTCACTCCAGCA+TGG - chr2.1:70247930-70247949 MS.gene002526:intron 50.0%
AGGTCCAGAAGCAACCTCAA+AGG + chr2.1:70247826-70247845 None:intergenic 50.0%
ATGGTTCCTGCTCAACCACA+TGG - chr2.1:70246907-70246926 MS.gene002526:intron 50.0%
CACACGTGACCCAAAAACCA+TGG + chr2.1:70247639-70247658 None:intergenic 50.0%
CCAGTGCAACATCATGGACA+AGG - chr2.1:70246826-70246845 MS.gene002526:intron 50.0%
CCTGCAACACTCATCACCAA+TGG - chr2.1:70246053-70246072 MS.gene002526:intron 50.0%
CCTTGTCCATGATGTTGCAC+TGG + chr2.1:70246829-70246848 None:intergenic 50.0%
GGTCAGCAACACCATAACAC+TGG - chr2.1:70246928-70246947 MS.gene002526:CDS 50.0%
GTAGCTGGTGCAGTTACTTG+TGG + chr2.1:70246790-70246809 None:intergenic 50.0%
TGGTTGAGCAGGAACCATGT+TGG + chr2.1:70246905-70246924 None:intergenic 50.0%
TGTTCAGATGTGGGGGGATT+TGG + chr2.1:70247056-70247075 None:intergenic 50.0%
! TCACGTGTGCATGGCTCTAT+TGG - chr2.1:70247650-70247669 MS.gene002526:CDS 50.0%
!! ATCTCAGGCATTGGTGAACC+AGG - chr2.1:70247762-70247781 MS.gene002526:CDS 50.0%
!! CCATTGGTGATGAGTGTTGC+AGG + chr2.1:70246056-70246075 None:intergenic 50.0%
!! GTGATGGTGGAGGAAGTAGT+AGG + chr2.1:70247120-70247139 None:intergenic 50.0%
!!! GTTTTTGGGTCACGTGTGCA+TGG - chr2.1:70247641-70247660 MS.gene002526:CDS 50.0%
CACAGAACCCCTTACTCCTC+AGG - chr2.1:70246115-70246134 MS.gene002526:intron 55.0%
GAAGTCCTGAGCCAAAGCCA+AGG - chr2.1:70246299-70246318 MS.gene002526:CDS 55.0%
GCTGGTGCAGTTACTTGTGG+TGG + chr2.1:70246787-70246806 None:intergenic 55.0%
GGTGCAGTTACTTGTGGTGG+TGG + chr2.1:70246784-70246803 None:intergenic 55.0%
TGCTGACCATGTGGTTGAGC+AGG + chr2.1:70246916-70246935 None:intergenic 55.0%
!! GTGGCCTTTGAGGTTGCTTC+TGG - chr2.1:70247819-70247838 MS.gene002526:intron 55.0%
!! TTCCACCTTGGCTTTGGCTC+AGG + chr2.1:70246307-70246326 None:intergenic 55.0%
GTCCTGAGCCAAAGCCAAGG+TGG - chr2.1:70246302-70246321 MS.gene002526:CDS 60.0%
! GTAGTAGGAGTTGTGGCGGG+TGG + chr2.1:70247087-70247106 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 70246007 70248092 70246007 ID=MS.gene002526
chr2.1 mRNA 70246007 70248092 70246007 ID=MS.gene002526.t1;Parent=MS.gene002526
chr2.1 exon 70247963 70248092 70247963 ID=MS.gene002526.t1.exon1;Parent=MS.gene002526.t1
chr2.1 CDS 70247963 70248092 70247963 ID=cds.MS.gene002526.t1;Parent=MS.gene002526.t1
chr2.1 exon 70246928 70247812 70246928 ID=MS.gene002526.t1.exon2;Parent=MS.gene002526.t1
chr2.1 CDS 70246928 70247812 70246928 ID=cds.MS.gene002526.t1;Parent=MS.gene002526.t1
chr2.1 exon 70246123 70246330 70246123 ID=MS.gene002526.t1.exon3;Parent=MS.gene002526.t1
chr2.1 CDS 70246123 70246330 70246123 ID=cds.MS.gene002526.t1;Parent=MS.gene002526.t1
chr2.1 exon 70246007 70246034 70246007 ID=MS.gene002526.t1.exon4;Parent=MS.gene002526.t1
chr2.1 CDS 70246007 70246034 70246007 ID=cds.MS.gene002526.t1;Parent=MS.gene002526.t1
Gene Sequence

>MS.gene002526

ATGGCTTCATCAAATAGACACTGGCCAAGCATGTTTAAGTCTAAGCCCTGCAACACTCATCACCAATGGCAGCATGACATCAACTCTTCTCTCATCTCATCTGCTTGTCACAGAACCCCTTACTCCTCAGGTAATTATCTACATTTTCAATCTAACTATACTAATATTTGACTATAGAAAAACACTGATTCAACTTTTGTACGTAAAATATAGTTAGAGACCCTAATGTCTAATTTGTAACAAGTTATTATAGACATTTTTCTTCTTCTTGTGGAAATAGGTTGTGAAGAGAGAAGTCCTGAGCCAAAGCCAAGGTGGAATCCAAAACCAGAACAAATCCGCATACTTGAAGCTATCTTCAATTCTGGAATGGTGAATCCACCAAGAGAAGAGATAAGGAAGATTAGAGCACAGTTGCAAGAGTATGGTCAAGTTGGTGATGCTAATGTTTTCTATTGGTTCCAAAACCGCAAATCAAGAAGCAAGCATAAGCTTCGCCATCTCCAAAACCAGAACAAGAATCAGAACCAGAATCAGAATCAGAATCAGGATCAATCACCACAACAAAACCCTAATTCACTTCCTCAAATTTCTGCTACTGTACCTTCATCTTCTTCATCTTCCTCTGAGAAATCTCCACCAAAAGAATTAATACCTGCCAAAGTATTTTCCCTTGGTTTCCCTAACATCAATGATGTTGTGCCTAATTCTCCAACTGCTTCTGTGAACCAAAACCAAACATATTTTCAGCCTCAAAATGTAACTACCCTTCTTCCACCACCACAAGTAACTGCACCAGCTACTGAGACTTTCTTCTTCCCAGTGCAACATCATGGACAAGGAATAGTACTTCCTAACAATGTTACATCACAAGGGTTTTGCTTTTCTGAACTTTCCAACATGGTTCCTGCTCAACCACATGGTCAGCAACACCATAACACTGGTAACTGCACTACTAGTTTCCTGCTTAGTGAGATCATGAACAACCATGGTGTTAATTCCTCAAAGAAAGATCAAGAACAGGATAAGTCTGTGAAAATAATGCACCAAATCCCCCCACATCTGAACATCTGTTTGCCACCCGCCACAACTCCTACTACTGTTTTACATCCTACTACTTCCTCCACCATCACTGTTCAATCACCTATCATTTCTCAAGTGCAAGGTAATTAATTACATATATAGCATTTATTTACTTATGCAGTTACAGAAAAAGTTGAAATAATGATATATAGACAAAAATAAAATAAAATTTGACCCCAAATTATTTAGTTTCTTTAACACTACAATTCCACATATTTTCATCTCTAAATTTTTGTTGCTATTTTAACATGTATAGTATTCAAAACTTCTATTCTGCATAGTAATTTTTTATTGTTTATATATTTTAAGAAAAGTTTTTATATTAACACATCTTGTAAGTGCAAGAGCAAAACCACAAGATCTTGACTAGTTTATTTTTTTATTTTTCTTTTAGGTTACTCCTATTATTTCATCTATATACTTGTTAATTTTACACCGCCTATAGAACCTAAGAACATAGTTTTTGAGATAAAAATGGTAATGTATGTATGGAAAAGAAGAGAAAGTGGGTAATAAGATTAATGTGAATAAGACGTGGTTAGTGTACCATGGTTTTTGGGTCACGTGTGCATGGCTCTATTGGCTTAAATATAGGCTTTAGTATTAGTTTGACTTTTGGTAGCTATAGTTAAGGTTTGTATTTAGACTTGCACATGAGAAATGATTGAGTGTATCTCAGGCATTGGTGAACCAGGTGTTGCTGCAAAATCAGTGGTGTTTATCAATGATGTGGCCTTTGAGGTTGCTTCTGGACCTTTTAATGTTCGTGCGGCATTTGGGGATGATGCTGTTCTTGTACATCAAACTGGTCAGCCTGTGTTAACAAACCAGTATGGTGTCACTCTTCACTCACTCCAGCATGGTGCATGTTATTTTCTTGTAAGCACGTAAACATACATTATTTTATATACTTGTTTAGTTAATCTTATCAAATTAACCTTCTCTAATTTATTTTTCATATATTTTGTTTTGCAGATGTAGGGGAAAAAATGCTGGAGAGTGA
Protein sequence

>MS.gene002526.t1

MASSNRHWPSMFKSKPCNTHHQWQHDINSSLISSACHRTPYSSGCEERSPEPKPRWNPKPEQIRILEAIFNSGMVNPPREEIRKIRAQLQEYGQVGDANVFYWFQNRKSRSKHKLRHLQNQNKNQNQNQNQNQDQSPQQNPNSLPQISATVPSSSSSSSEKSPPKELIPAKVFSLGFPNINDVVPNSPTASVNQNQTYFQPQNVTTLLPPPQVTAPATETFFFPVQHHGQGIVLPNNVTSQGFCFSELSNMVPAQPHGQQHHNTGNCTTSFLLSEIMNNHGVNSSKKDQEQDKSVKIMHQIPPHLNICLPPATTPTTVLHPTTSSTITVQSPIISQVQGIGEPGVAAKSVVFINDVAFEVASGPFNVRAAFGDDAVLVHQTGQPVLTNQYGVTLHSLQHGACYFLVSTCRGKNAGE