Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002629.t1 | XP_003593824.2 | 80.2 | 323 | 58 | 3 | 1 | 318 | 34 | 355 | 6.90E-116 | 427.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002629.t1 | Q8VZN1 | 32.3 | 263 | 147 | 8 | 46 | 304 | 112 | 347 | 8.7e-19 | 95.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002629.t1 | G7ILU8 | 80.2 | 323 | 58 | 3 | 1 | 318 | 35 | 356 | 4.9e-116 | 427.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene002629.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002629.t1 | MTR_2g018030 | 93.498 | 323 | 15 | 3 | 1 | 318 | 35 | 356 | 0.0 | 585 |
| MS.gene002629.t1 | MTR_8g087530 | 43.774 | 265 | 107 | 6 | 56 | 320 | 117 | 339 | 7.90e-56 | 184 |
| MS.gene002629.t1 | MTR_7g072875 | 38.255 | 149 | 83 | 3 | 160 | 304 | 141 | 284 | 1.48e-26 | 106 |
| MS.gene002629.t1 | MTR_7g072630 | 38.255 | 149 | 83 | 3 | 160 | 304 | 203 | 346 | 3.65e-26 | 106 |
| MS.gene002629.t1 | MTR_4g028930 | 38.509 | 161 | 92 | 2 | 149 | 307 | 227 | 382 | 1.21e-25 | 105 |
| MS.gene002629.t1 | MTR_5g075210 | 34.320 | 169 | 82 | 6 | 147 | 305 | 197 | 346 | 6.46e-23 | 97.8 |
| MS.gene002629.t1 | MTR_8g103520 | 51.613 | 62 | 30 | 0 | 239 | 300 | 224 | 285 | 3.56e-16 | 77.8 |
| MS.gene002629.t1 | MTR_0907s0020 | 41.026 | 78 | 42 | 1 | 242 | 319 | 105 | 178 | 1.49e-13 | 68.6 |
| MS.gene002629.t1 | MTR_4g132740 | 39.683 | 63 | 38 | 0 | 241 | 303 | 83 | 145 | 1.30e-11 | 62.0 |
| MS.gene002629.t1 | MTR_4g052040 | 26.923 | 130 | 86 | 1 | 180 | 300 | 31 | 160 | 1.89e-11 | 62.4 |
| MS.gene002629.t1 | MTR_7g105790 | 42.857 | 63 | 36 | 0 | 242 | 304 | 113 | 175 | 3.15e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002629.t1 | AT4G18830 | 35.841 | 226 | 120 | 7 | 83 | 304 | 143 | 347 | 2.28e-31 | 120 |
| MS.gene002629.t1 | AT1G06920 | 65.152 | 66 | 23 | 0 | 238 | 303 | 242 | 307 | 5.10e-24 | 100 |
| MS.gene002629.t1 | AT2G30400 | 61.765 | 68 | 26 | 0 | 238 | 305 | 251 | 318 | 2.62e-23 | 98.2 |
| MS.gene002629.t1 | AT5G58360 | 44.444 | 90 | 49 | 1 | 221 | 310 | 206 | 294 | 1.58e-20 | 90.1 |
| MS.gene002629.t1 | AT5G01840 | 56.452 | 62 | 27 | 0 | 243 | 304 | 202 | 263 | 1.39e-19 | 87.0 |
| MS.gene002629.t1 | AT5G19650 | 46.774 | 62 | 33 | 0 | 239 | 300 | 154 | 215 | 1.68e-14 | 72.0 |
| MS.gene002629.t1 | AT2G18500 | 40.909 | 66 | 39 | 0 | 238 | 303 | 225 | 290 | 4.71e-13 | 69.3 |
| MS.gene002629.t1 | AT3G52525 | 46.032 | 63 | 34 | 0 | 240 | 302 | 67 | 129 | 2.40e-12 | 64.3 |
| MS.gene002629.t1 | AT4G04025 | 41.176 | 68 | 40 | 0 | 232 | 299 | 106 | 173 | 6.49e-11 | 60.8 |
Find 40 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAAGGAGGAAGATATTAT+TGG | 0.269462 | 2.1:-68944667 | MS.gene002629:CDS |
| GAGGAAAGCACAACAGGTTT+TGG | 0.290055 | 2.1:-68944536 | MS.gene002629:CDS |
| TGGTTTGATATGAGTCAATA+TGG | 0.296873 | 2.1:-68943899 | MS.gene002629:CDS |
| TTCACCCTTGAAACTTTCTT+TGG | 0.300747 | 2.1:+68944238 | None:intergenic |
| TTTGATATTGGTGGAAAATA+TGG | 0.307283 | 2.1:-68944724 | MS.gene002629:CDS |
| TAATTATGAGATGCATAGTT+TGG | 0.307924 | 2.1:-68944311 | MS.gene002629:CDS |
| AAAAGGAATATGAAAATATA+AGG | 0.317179 | 2.1:-68944570 | MS.gene002629:CDS |
| ACAAGGTATAGTCAAGTTAA+AGG | 0.319980 | 2.1:-68944683 | MS.gene002629:CDS |
| GATATGAGTCAATATGGTTT+AGG | 0.337600 | 2.1:-68943893 | MS.gene002629:CDS |
| GAAAATATATTCACCAAGAT+TGG | 0.341908 | 2.1:-68944218 | MS.gene002629:CDS |
| ACAGCAAAACTATCTAATTC+TGG | 0.358668 | 2.1:+68944091 | None:intergenic |
| TAATTGGTAATTATCATTCA+TGG | 0.367760 | 2.1:+68943843 | None:intergenic |
| GCAAAAGAATGTGTTGTTCT+TGG | 0.370662 | 2.1:+68944427 | None:intergenic |
| GATTAGTAAGGCAGAAGAAA+TGG | 0.373351 | 2.1:-68943999 | MS.gene002629:CDS |
| CTTGGTGTACAAATTGTTCT+TGG | 0.377176 | 2.1:+68944445 | None:intergenic |
| ATCTCATAATCAAAGTATTT+AGG | 0.389618 | 2.1:-68943928 | MS.gene002629:CDS |
| TCAACCTTTGAAACCAATCT+TGG | 0.392383 | 2.1:+68944205 | None:intergenic |
| GAATCTCTAAAATCCTGCTT+TGG | 0.398970 | 2.1:+68944049 | None:intergenic |
| ATTTGAGAGGAAAGCACAAC+AGG | 0.409645 | 2.1:-68944542 | MS.gene002629:CDS |
| GGAAAATATGGTAACAAAAG+TGG | 0.450154 | 2.1:-68944712 | MS.gene002629:CDS |
| TCAAAGTATTTAGGCAGGTA+TGG | 0.455774 | 2.1:-68943919 | MS.gene002629:CDS |
| AGAAGAAATGGAAGAGTTGT+TGG | 0.458484 | 2.1:-68943987 | MS.gene002629:CDS |
| GGAAAAGCATGAAGAGTTGA+AGG | 0.459523 | 2.1:-68944290 | MS.gene002629:CDS |
| AAAGATGAAGAAAGAAATTG+TGG | 0.494347 | 2.1:-68944134 | MS.gene002629:CDS |
| TGAAGAGAAACAGATTAGTA+AGG | 0.497006 | 2.1:-68944011 | MS.gene002629:CDS |
| GGAAACCAAAGAAAGTTTCA+AGG | 0.508120 | 2.1:-68944243 | MS.gene002629:CDS |
| TTCACCAAGATTGGTTTCAA+AGG | 0.510346 | 2.1:-68944209 | MS.gene002629:CDS |
| GTATGAATATGATAGAGAAA+AGG | 0.539540 | 2.1:-68944587 | MS.gene002629:CDS |
| CATAATCAAAGTATTTAGGC+AGG | 0.542446 | 2.1:-68943924 | MS.gene002629:CDS |
| CCACCAATATCAAAACATGA+AGG | 0.542576 | 2.1:+68944733 | None:intergenic |
| ATGTTCATCAAATCCAAAGC+AGG | 0.550210 | 2.1:-68944062 | MS.gene002629:CDS |
| TAAAAGCATTAGAAGAAATG+AGG | 0.552124 | 2.1:-68944171 | MS.gene002629:CDS |
| GAAACCAAAGAAAGTTTCAA+GGG | 0.604643 | 2.1:-68944242 | MS.gene002629:CDS |
| GATGAAGAAAGAAATTGTGG+AGG | 0.614642 | 2.1:-68944131 | MS.gene002629:CDS |
| CCAATATCAAAACATGAAGG+TGG | 0.616765 | 2.1:+68944736 | None:intergenic |
| AAAATGGTGAATGATAAGAA+GGG | 0.623597 | 2.1:-68944628 | MS.gene002629:CDS |
| ATATAAGGAGAAGATTTGAG+AGG | 0.626627 | 2.1:-68944555 | MS.gene002629:CDS |
| AGGTATAGTCAAGTTAAAGG+AGG | 0.630076 | 2.1:-68944680 | MS.gene002629:CDS |
| AACAAAAGTGGAAGAAAACA+AGG | 0.640173 | 2.1:-68944700 | MS.gene002629:CDS |
| GTTAGATCAGAAATGCAATG+CGG | 0.769031 | 2.1:-68943869 | MS.gene002629:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGGAATATGAAAATATA+AGG | - | chr2.1:68944082-68944101 | MS.gene002629:CDS | 15.0% |
| !! | TTTAGAAGATCAAAGAAAAA+TGG | - | chr2.1:68944008-68944027 | MS.gene002629:CDS | 20.0% |
| !!! | ATCTCATAATCAAAGTATTT+AGG | - | chr2.1:68944724-68944743 | MS.gene002629:CDS | 20.0% |
| ! | AAAAATGGTGAATGATAAGA+AGG | - | chr2.1:68944023-68944042 | MS.gene002629:CDS | 25.0% |
| ! | AAAATGGTGAATGATAAGAA+GGG | - | chr2.1:68944024-68944043 | MS.gene002629:CDS | 25.0% |
| ! | AAAGATGAAGAAAGAAATTG+TGG | - | chr2.1:68944518-68944537 | MS.gene002629:CDS | 25.0% |
| ! | GAAAATATATTCACCAAGAT+TGG | - | chr2.1:68944434-68944453 | MS.gene002629:CDS | 25.0% |
| ! | GTATGAATATGATAGAGAAA+AGG | - | chr2.1:68944065-68944084 | MS.gene002629:CDS | 25.0% |
| ! | TAAAGACTCAAGAAAAACAA+AGG | - | chr2.1:68944385-68944404 | MS.gene002629:CDS | 25.0% |
| ! | TAATTATGAGATGCATAGTT+TGG | - | chr2.1:68944341-68944360 | MS.gene002629:CDS | 25.0% |
| ! | TTAAAGGAGGAAGATATTAT+TGG | - | chr2.1:68943985-68944004 | MS.gene002629:CDS | 25.0% |
| ! | TTTGATATTGGTGGAAAATA+TGG | - | chr2.1:68943928-68943947 | MS.gene002629:CDS | 25.0% |
| !!! | TAAAAGCATTAGAAGAAATG+AGG | - | chr2.1:68944481-68944500 | MS.gene002629:CDS | 25.0% |
| AACAAAAGTGGAAGAAAACA+AGG | - | chr2.1:68943952-68943971 | MS.gene002629:CDS | 30.0% | |
| ACAAGGTATAGTCAAGTTAA+AGG | - | chr2.1:68943969-68943988 | MS.gene002629:CDS | 30.0% | |
| ACAGCAAAACTATCTAATTC+TGG | + | chr2.1:68944564-68944583 | None:intergenic | 30.0% | |
| ATATAAGGAGAAGATTTGAG+AGG | - | chr2.1:68944097-68944116 | MS.gene002629:CDS | 30.0% | |
| GAAACCAAAGAAAGTTTCAA+GGG | - | chr2.1:68944410-68944429 | MS.gene002629:CDS | 30.0% | |
| GATATGAGTCAATATGGTTT+AGG | - | chr2.1:68944759-68944778 | MS.gene002629:CDS | 30.0% | |
| GGAAAATATGGTAACAAAAG+TGG | - | chr2.1:68943940-68943959 | MS.gene002629:CDS | 30.0% | |
| TGAAGAGAAACAGATTAGTA+AGG | - | chr2.1:68944641-68944660 | MS.gene002629:CDS | 30.0% | |
| TGGTTTGATATGAGTCAATA+TGG | - | chr2.1:68944753-68944772 | MS.gene002629:CDS | 30.0% | |
| ! | CATAATCAAAGTATTTAGGC+AGG | - | chr2.1:68944728-68944747 | MS.gene002629:CDS | 30.0% |
| AGAAGAAATGGAAGAGTTGT+TGG | - | chr2.1:68944665-68944684 | MS.gene002629:CDS | 35.0% | |
| AGACTCAAGAAAAACAAAGG+AGG | - | chr2.1:68944388-68944407 | MS.gene002629:CDS | 35.0% | |
| AGGTATAGTCAAGTTAAAGG+AGG | - | chr2.1:68943972-68943991 | MS.gene002629:CDS | 35.0% | |
| ATGTTCATCAAATCCAAAGC+AGG | - | chr2.1:68944590-68944609 | MS.gene002629:CDS | 35.0% | |
| CCAATATCAAAACATGAAGG+TGG | + | chr2.1:68943919-68943938 | None:intergenic | 35.0% | |
| CCACCAATATCAAAACATGA+AGG | + | chr2.1:68943922-68943941 | None:intergenic | 35.0% | |
| GATGAAGAAAGAAATTGTGG+AGG | - | chr2.1:68944521-68944540 | MS.gene002629:CDS | 35.0% | |
| GATTAGTAAGGCAGAAGAAA+TGG | - | chr2.1:68944653-68944672 | MS.gene002629:CDS | 35.0% | |
| GGAAACCAAAGAAAGTTTCA+AGG | - | chr2.1:68944409-68944428 | MS.gene002629:CDS | 35.0% | |
| GTTAGATCAGAAATGCAATG+CGG | - | chr2.1:68944783-68944802 | MS.gene002629:CDS | 35.0% | |
| TTCACCCTTGAAACTTTCTT+TGG | + | chr2.1:68944417-68944436 | None:intergenic | 35.0% | |
| ! | CTTGGTGTACAAATTGTTCT+TGG | + | chr2.1:68944210-68944229 | None:intergenic | 35.0% |
| ! | GCAAAAGAATGTGTTGTTCT+TGG | + | chr2.1:68944228-68944247 | None:intergenic | 35.0% |
| ! | TCAAAGTATTTAGGCAGGTA+TGG | - | chr2.1:68944733-68944752 | MS.gene002629:CDS | 35.0% |
| !! | GAATCTCTAAAATCCTGCTT+TGG | + | chr2.1:68944606-68944625 | None:intergenic | 35.0% |
| !! | TCAACCTTTGAAACCAATCT+TGG | + | chr2.1:68944450-68944469 | None:intergenic | 35.0% |
| !! | TTCACCAAGATTGGTTTCAA+AGG | - | chr2.1:68944443-68944462 | MS.gene002629:CDS | 35.0% |
| !!! | CCACCTTCATGTTTTGATAT+TGG | - | chr2.1:68943916-68943935 | MS.gene002629:CDS | 35.0% |
| !!! | CCTTCATGTTTTGATATTGG+TGG | - | chr2.1:68943919-68943938 | MS.gene002629:CDS | 35.0% |
| ! | ATTTGAGAGGAAAGCACAAC+AGG | - | chr2.1:68944110-68944129 | MS.gene002629:CDS | 40.0% |
| ! | GGAAAAGCATGAAGAGTTGA+AGG | - | chr2.1:68944362-68944381 | MS.gene002629:CDS | 40.0% |
| !! | GAGGAAAGCACAACAGGTTT+TGG | - | chr2.1:68944116-68944135 | MS.gene002629:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 68943856 | 68944818 | 68943856 | ID=MS.gene002629 |
| chr2.1 | mRNA | 68943856 | 68944818 | 68943856 | ID=MS.gene002629.t1;Parent=MS.gene002629 |
| chr2.1 | exon | 68943856 | 68944818 | 68943856 | ID=MS.gene002629.t1.exon1;Parent=MS.gene002629.t1 |
| chr2.1 | CDS | 68943856 | 68944818 | 68943856 | ID=cds.MS.gene002629.t1;Parent=MS.gene002629.t1 |
| Gene Sequence |
| Protein sequence |
