Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002717.t1 | XP_013441754.1 | 91.1 | 214 | 19 | 0 | 1 | 214 | 1 | 214 | 2.90E-108 | 401.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002717.t1 | A0A072TE13 | 91.1 | 214 | 19 | 0 | 1 | 214 | 1 | 214 | 2.1e-108 | 401.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene002717.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene002717.t1 | MTR_0558s0020 | 91.121 | 214 | 19 | 0 | 1 | 214 | 1 | 214 | 1.15e-146 | 410 |
| MS.gene002717.t1 | MTR_1g083640 | 86.900 | 229 | 15 | 1 | 1 | 214 | 1 | 229 | 5.81e-146 | 409 |
| MS.gene002717.t1 | MTR_2g014100 | 84.685 | 222 | 26 | 1 | 1 | 214 | 1 | 222 | 5.20e-137 | 384 |
| MS.gene002717.t1 | MTR_3g008240 | 89.163 | 203 | 22 | 0 | 1 | 203 | 1 | 203 | 5.33e-135 | 377 |
| MS.gene002717.t1 | MTR_4g040430 | 89.163 | 203 | 17 | 2 | 1 | 203 | 1 | 198 | 2.91e-132 | 370 |
| MS.gene002717.t1 | MTR_5g071590 | 83.333 | 180 | 18 | 1 | 1 | 168 | 1 | 180 | 2.18e-109 | 312 |
| MS.gene002717.t1 | MTR_8g446020 | 72.897 | 214 | 18 | 2 | 1 | 214 | 1 | 174 | 3.54e-104 | 301 |
| MS.gene002717.t1 | MTR_6g090505 | 87.919 | 149 | 18 | 0 | 66 | 214 | 1 | 149 | 1.02e-93 | 279 |
| MS.gene002717.t1 | MTR_0337s0040 | 65.421 | 214 | 35 | 1 | 1 | 214 | 1 | 175 | 6.48e-93 | 272 |
| MS.gene002717.t1 | MTR_0019s0290 | 85.606 | 132 | 19 | 0 | 83 | 214 | 9 | 140 | 1.24e-80 | 238 |
| MS.gene002717.t1 | MTR_1g055240 | 89.516 | 124 | 13 | 0 | 91 | 214 | 2 | 125 | 6.82e-80 | 236 |
| MS.gene002717.t1 | MTR_1281s0010 | 94.495 | 109 | 6 | 0 | 20 | 128 | 1 | 109 | 8.75e-73 | 217 |
| MS.gene002717.t1 | MTR_4g008820 | 87.500 | 80 | 10 | 0 | 1 | 80 | 125 | 204 | 4.11e-40 | 145 |
| MS.gene002717.t1 | MTR_8g046060 | 81.818 | 88 | 8 | 2 | 1 | 80 | 91 | 178 | 1.25e-38 | 133 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTAAGTCTGAAAATCGAAAA+AGG | 0.290224 | 2.1:+67916964 | None:intergenic |
| TCTGATGTTGGCCTGTTGTT+AGG | 0.302249 | 2.1:+67917282 | None:intergenic |
| TCTCATCGACATTCCAGCTT+TGG | 0.379651 | 2.1:+67917035 | None:intergenic |
| GAGAACGTCTTATGAAGTTA+TGG | 0.394356 | 2.1:-67917016 | MS.gene002717:CDS |
| GAAAGGGTTGGTTGTAAAGC+TGG | 0.415036 | 2.1:-67917213 | MS.gene002717:CDS |
| GAAGTTATGGGTCAAGGAGT+TGG | 0.428685 | 2.1:-67917003 | MS.gene002717:CDS |
| CGAGAAGTAAATGAAGTTCA+AGG | 0.430014 | 2.1:-67917078 | MS.gene002717:CDS |
| ATGGGTATAAGATATATAGA+TGG | 0.430188 | 2.1:-67917161 | MS.gene002717:CDS |
| TCTAATGACAATTCGTCAAA+AGG | 0.456711 | 2.1:-67916934 | MS.gene002717:CDS |
| GCTCTAATAAATTCCAAAGC+TGG | 0.462891 | 2.1:-67917048 | MS.gene002717:CDS |
| TCCATGATTTGTTATTGCTA+GGG | 0.463437 | 2.1:+67917493 | None:intergenic |
| AGAACGTCTTATGAAGTTAT+GGG | 0.466325 | 2.1:-67917015 | MS.gene002717:CDS |
| CCCCTAGCAATAACAAATCA+TGG | 0.469857 | 2.1:-67917494 | MS.gene002717:CDS |
| AGCTGGATTATCTTTGAAGC+TGG | 0.474766 | 2.1:-67917196 | MS.gene002717:CDS |
| GTTTGCTTAATCCTAACAAC+AGG | 0.484661 | 2.1:-67917293 | MS.gene002717:CDS |
| TTACTTCTCGAAATGCTCTC+AGG | 0.486887 | 2.1:+67917091 | None:intergenic |
| CGTTATGTTTGTAACAAACA+AGG | 0.494754 | 2.1:-67917330 | MS.gene002717:CDS |
| CTCAGGTAACACAAATGCTC+TGG | 0.496027 | 2.1:+67917108 | None:intergenic |
| TTACAACCAACCCTTTCCTC+AGG | 0.505082 | 2.1:+67917219 | None:intergenic |
| TTCCATGATTTGTTATTGCT+AGG | 0.510433 | 2.1:+67917492 | None:intergenic |
| TAACAAACAAGGTTTCAAGA+AGG | 0.511682 | 2.1:-67917319 | MS.gene002717:CDS |
| AAATGCTCTGGTTTATGTAA+TGG | 0.514358 | 2.1:+67917120 | None:intergenic |
| GGTAATTATGCATATAACAC+TGG | 0.523547 | 2.1:-67917426 | MS.gene002717:CDS |
| TGGGTATAAGATATATAGAT+GGG | 0.523615 | 2.1:-67917160 | MS.gene002717:CDS |
| ACAAAATAAGAAATCAGATA+AGG | 0.530342 | 2.1:-67917364 | MS.gene002717:CDS |
| CCATGATTTGTTATTGCTAG+GGG | 0.534941 | 2.1:+67917494 | None:intergenic |
| AACAAACAAGGTTTCAAGAA+GGG | 0.554561 | 2.1:-67917318 | MS.gene002717:CDS |
| CGTCAAAAGGAGATGGTGGT+TGG | 0.556443 | 2.1:-67916921 | MS.gene002717:CDS |
| GACAATTCGTCAAAAGGAGA+TGG | 0.566160 | 2.1:-67916928 | MS.gene002717:CDS |
| TAAGATATATAGATGGGATG+TGG | 0.566406 | 2.1:-67917154 | MS.gene002717:CDS |
| GTTATGGGTCAAGGAGTTGG+AGG | 0.578191 | 2.1:-67917000 | MS.gene002717:CDS |
| TCTTATGAAGTTATGGGTCA+AGG | 0.580120 | 2.1:-67917009 | MS.gene002717:CDS |
| ATATCAACTTCATTTGAGAG+TGG | 0.594340 | 2.1:-67917573 | MS.gene002717:CDS |
| GCCAACATCAGACAGTCCAC+TGG | 0.611268 | 2.1:-67917271 | MS.gene002717:CDS |
| ACCAGTGGACTGTCTGATGT+TGG | 0.614292 | 2.1:+67917270 | None:intergenic |
| TTCACAAACTCAATAACCAG+TGG | 0.633415 | 2.1:+67917255 | None:intergenic |
| AATTCGTCAAAAGGAGATGG+TGG | 0.636402 | 2.1:-67916925 | MS.gene002717:CDS |
| GGGTCAAGGAGTTGGAGGCA+CGG | 0.637387 | 2.1:-67916995 | MS.gene002717:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATAAGAAATCAGATA+AGG | - | chr2.1:67917136-67917155 | MS.gene002717:CDS | 20.0% |
| !!! | AAAGAGATTGAAGATTTTTA+TGG | - | chr2.1:67917053-67917072 | MS.gene002717:CDS | 20.0% |
| ! | ATGGGTATAAGATATATAGA+TGG | - | chr2.1:67917339-67917358 | MS.gene002717:CDS | 25.0% |
| ! | TGGGTATAAGATATATAGAT+GGG | - | chr2.1:67917340-67917359 | MS.gene002717:CDS | 25.0% |
| ! | TTAAGTCTGAAAATCGAAAA+AGG | + | chr2.1:67917539-67917558 | None:intergenic | 25.0% |
| AACAAACAAGGTTTCAAGAA+GGG | - | chr2.1:67917182-67917201 | MS.gene002717:CDS | 30.0% | |
| AGAACGTCTTATGAAGTTAT+GGG | - | chr2.1:67917485-67917504 | MS.gene002717:CDS | 30.0% | |
| ATATCAACTTCATTTGAGAG+TGG | - | chr2.1:67916927-67916946 | MS.gene002717:CDS | 30.0% | |
| CGTTATGTTTGTAACAAACA+AGG | - | chr2.1:67917170-67917189 | MS.gene002717:CDS | 30.0% | |
| GGTAATTATGCATATAACAC+TGG | - | chr2.1:67917074-67917093 | MS.gene002717:CDS | 30.0% | |
| TAACAAACAAGGTTTCAAGA+AGG | - | chr2.1:67917181-67917200 | MS.gene002717:CDS | 30.0% | |
| TAAGATATATAGATGGGATG+TGG | - | chr2.1:67917346-67917365 | MS.gene002717:CDS | 30.0% | |
| TCTAATGACAATTCGTCAAA+AGG | - | chr2.1:67917566-67917585 | MS.gene002717:CDS | 30.0% | |
| ! | AAATGCTCTGGTTTATGTAA+TGG | + | chr2.1:67917383-67917402 | None:intergenic | 30.0% |
| !! | TCCATGATTTGTTATTGCTA+GGG | + | chr2.1:67917010-67917029 | None:intergenic | 30.0% |
| !! | TTCCATGATTTGTTATTGCT+AGG | + | chr2.1:67917011-67917030 | None:intergenic | 30.0% |
| CGAGAAGTAAATGAAGTTCA+AGG | - | chr2.1:67917422-67917441 | MS.gene002717:CDS | 35.0% | |
| GAAAGAAAAACCTGAGGAAA+GGG | - | chr2.1:67917271-67917290 | MS.gene002717:CDS | 35.0% | |
| GAAAGTGAAAGAAAAACCTG+AGG | - | chr2.1:67917265-67917284 | MS.gene002717:CDS | 35.0% | |
| GAGAACGTCTTATGAAGTTA+TGG | - | chr2.1:67917484-67917503 | MS.gene002717:CDS | 35.0% | |
| GCTCTAATAAATTCCAAAGC+TGG | - | chr2.1:67917452-67917471 | MS.gene002717:CDS | 35.0% | |
| GTTTGCTTAATCCTAACAAC+AGG | - | chr2.1:67917207-67917226 | MS.gene002717:CDS | 35.0% | |
| TCTTATGAAGTTATGGGTCA+AGG | - | chr2.1:67917491-67917510 | MS.gene002717:CDS | 35.0% | |
| TGAAAGAAAAACCTGAGGAA+AGG | - | chr2.1:67917270-67917289 | MS.gene002717:CDS | 35.0% | |
| TTCACAAACTCAATAACCAG+TGG | + | chr2.1:67917248-67917267 | None:intergenic | 35.0% | |
| !! | CCATGATTTGTTATTGCTAG+GGG | + | chr2.1:67917009-67917028 | None:intergenic | 35.0% |
| !!! | AAGCTGGATGAAGTTTTGAA+TGG | - | chr2.1:67917320-67917339 | MS.gene002717:CDS | 35.0% |
| !!! | AGCTGGATGAAGTTTTGAAT+GGG | - | chr2.1:67917321-67917340 | MS.gene002717:CDS | 35.0% |
| AATTCGTCAAAAGGAGATGG+TGG | - | chr2.1:67917575-67917594 | MS.gene002717:CDS | 40.0% | |
| CCCCTAGCAATAACAAATCA+TGG | - | chr2.1:67917006-67917025 | MS.gene002717:CDS | 40.0% | |
| GACAATTCGTCAAAAGGAGA+TGG | - | chr2.1:67917572-67917591 | MS.gene002717:CDS | 40.0% | |
| TTACTTCTCGAAATGCTCTC+AGG | + | chr2.1:67917412-67917431 | None:intergenic | 40.0% | |
| !! | AGCTGGATTATCTTTGAAGC+TGG | - | chr2.1:67917304-67917323 | MS.gene002717:CDS | 40.0% |
| CTCAGGTAACACAAATGCTC+TGG | + | chr2.1:67917395-67917414 | None:intergenic | 45.0% | |
| GAAAAACCTGAGGAAAGGGT+TGG | - | chr2.1:67917275-67917294 | MS.gene002717:CDS | 45.0% | |
| GAAGTTATGGGTCAAGGAGT+TGG | - | chr2.1:67917497-67917516 | MS.gene002717:CDS | 45.0% | |
| TCTCATCGACATTCCAGCTT+TGG | + | chr2.1:67917468-67917487 | None:intergenic | 45.0% | |
| TTACAACCAACCCTTTCCTC+AGG | + | chr2.1:67917284-67917303 | None:intergenic | 45.0% | |
| ! | TCTGATGTTGGCCTGTTGTT+AGG | + | chr2.1:67917221-67917240 | None:intergenic | 45.0% |
| !! | GAAAGGGTTGGTTGTAAAGC+TGG | - | chr2.1:67917287-67917306 | MS.gene002717:CDS | 45.0% |
| GTTATGGGTCAAGGAGTTGG+AGG | - | chr2.1:67917500-67917519 | MS.gene002717:CDS | 50.0% | |
| ! | CGTCAAAAGGAGATGGTGGT+TGG | - | chr2.1:67917579-67917598 | MS.gene002717:CDS | 50.0% |
| !! | ACCAGTGGACTGTCTGATGT+TGG | + | chr2.1:67917233-67917252 | None:intergenic | 50.0% |
| GCCAACATCAGACAGTCCAC+TGG | - | chr2.1:67917229-67917248 | MS.gene002717:CDS | 55.0% | |
| GGGTCAAGGAGTTGGAGGCA+CGG | - | chr2.1:67917505-67917524 | MS.gene002717:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.1 | gene | 67916918 | 67917604 | 67916918 | ID=MS.gene002717 |
| chr2.1 | mRNA | 67916918 | 67917604 | 67916918 | ID=MS.gene002717.t1;Parent=MS.gene002717 |
| chr2.1 | exon | 67916918 | 67917604 | 67916918 | ID=MS.gene002717.t1.exon1;Parent=MS.gene002717.t1 |
| chr2.1 | CDS | 67916918 | 67917604 | 67916918 | ID=cds.MS.gene002717.t1;Parent=MS.gene002717.t1 |
| Gene Sequence |
| Protein sequence |
