AlfalfaGEDB | MS.gene002752

M. sativa cultivar XinJiangDaYe / MS.gene002752

KEGG: K15032
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02536.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002752.t1	XP_003593962.1	80.6	170	31	1	1	168	196	365	3.60E-72	281.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002752.t1	G7IPZ5	80.6	170	31	1	1	168	196	365	2.6e-72	281.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene002752.t1	TR	mTERF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002752.t1	MTR_2g019810	80.588	170	31	1	1	168	196	365	3.26e-98	289
MS.gene002752.t1	MTR_2g019840	73.964	169	42	1	1	167	208	376	4.13e-86	259
MS.gene002752.t1	MTR_2g436400	50.625	160	75	3	11	166	222	381	9.68e-49	162
MS.gene002752.t1	MTR_2g437960	46.524	187	91	4	1	178	216	402	1.37e-46	157
MS.gene002752.t1	MTR_2g437960	46.524	187	91	4	1	178	216	402	1.73e-46	158
MS.gene002752.t1	MTR_2g436460	47.429	175	82	5	1	165	205	379	2.02e-45	154
MS.gene002752.t1	MTR_2g437080	50.000	160	76	3	11	166	215	374	8.67e-45	152
MS.gene002752.t1	MTR_4g119550	48.125	160	79	3	11	166	225	384	4.10e-44	151
MS.gene002752.t1	MTR_2g437090	48.125	160	79	3	11	166	3	162	5.10e-44	144
MS.gene002752.t1	MTR_2g437030	50.000	160	76	3	11	166	301	460	5.34e-44	152
MS.gene002752.t1	MTR_2g437060	49.359	156	75	3	11	162	230	385	5.68e-42	145
MS.gene002752.t1	MTR_2g437240	43.850	187	96	4	1	178	228	414	6.70e-42	145
MS.gene002752.t1	MTR_2g437170	47.953	171	80	4	1	162	220	390	9.94e-42	145
MS.gene002752.t1	MTR_2g437200	48.538	171	79	4	1	162	228	398	1.26e-41	144
MS.gene002752.t1	MTR_2g437040	46.203	158	81	3	13	166	217	374	1.78e-41	144
MS.gene002752.t1	MTR_2g438010	41.711	187	100	4	1	178	233	419	2.55e-41	144
MS.gene002752.t1	MTR_2g437020	48.750	160	77	4	11	166	216	374	5.47e-41	142
MS.gene002752.t1	MTR_2g437150	45.143	175	87	4	1	166	220	394	6.60e-41	143
MS.gene002752.t1	MTR_2g436440	46.835	158	80	3	13	166	223	380	1.02e-40	142
MS.gene002752.t1	MTR_2g437160	48.684	152	74	3	15	162	153	304	1.60e-40	140
MS.gene002752.t1	MTR_2g437120	49.677	155	75	2	11	162	229	383	1.78e-40	142
MS.gene002752.t1	MTR_2g437940	46.199	171	83	4	1	162	184	354	2.63e-40	140
MS.gene002752.t1	MTR_2g438020	47.953	171	80	4	1	162	267	437	5.09e-40	142
MS.gene002752.t1	MTR_2g437160	48.684	152	74	3	15	162	244	395	8.92e-40	140
MS.gene002752.t1	MTR_2g437100	45.665	173	85	4	1	164	225	397	9.61e-39	137
MS.gene002752.t1	MTR_2g437260	43.605	172	86	5	1	161	194	365	1.94e-38	136
MS.gene002752.t1	MTR_4g119580	44.379	169	85	4	1	160	202	370	2.54e-38	135
MS.gene002752.t1	MTR_2g437990	43.023	172	94	3	11	178	227	398	2.98e-38	136
MS.gene002752.t1	MTR_2g437180	46.784	171	80	5	1	162	212	380	3.69e-38	135
MS.gene002752.t1	MTR_2g076320	45.513	156	81	3	11	162	228	383	6.15e-37	132
MS.gene002752.t1	MTR_2g437130	40.341	176	96	4	1	167	201	376	3.14e-36	130
MS.gene002752.t1	MTR_4g120380	40.828	169	91	4	1	160	202	370	5.51e-35	126
MS.gene002752.t1	MTR_4g120380	38.255	149	83	4	1	140	202	350	4.39e-26	103
MS.gene002752.t1	MTR_4g007490	35.220	159	99	3	9	163	213	371	4.88e-26	102
MS.gene002752.t1	MTR_3g450500	36.943	157	93	4	9	160	215	370	7.60e-26	102
MS.gene002752.t1	MTR_2g019830	70.149	67	18	1	101	165	136	202	2.83e-22	89.7
MS.gene002752.t1	MTR_8g080990	33.566	143	89	4	19	156	227	368	1.29e-14	71.2
MS.gene002752.t1	MTR_8g012210	22.905	179	121	6	1	165	178	353	1.20e-12	65.5
MS.gene002752.t1	MTR_3g085240	36.364	88	42	1	67	154	176	249	1.65e-12	64.3
MS.gene002752.t1	MTR_8g081000	28.477	151	102	4	15	160	236	385	2.30e-11	62.0
MS.gene002752.t1	MTR_8g081000	28.477	151	102	4	15	160	236	385	2.36e-11	62.0
MS.gene002752.t1	MTR_8g081000	28.477	151	102	4	15	160	236	385	2.42e-11	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene002752.t1	AT5G07900	39.103	156	90	3	9	159	244	399	4.56e-31	117
MS.gene002752.t1	AT1G21150	30.625	160	106	3	9	163	195	354	1.14e-20	88.2
MS.gene002752.t1	AT1G21150	30.625	160	106	3	9	163	231	390	1.87e-20	87.8
MS.gene002752.t1	AT1G21150	30.625	160	106	3	9	163	295	454	2.32e-20	88.2
MS.gene002752.t1	AT1G62010	26.341	205	104	7	9	172	207	405	1.50e-12	65.5
MS.gene002752.t1	AT1G61990	25.773	194	100	7	9	159	216	408	2.73e-12	64.7
MS.gene002752.t1	AT1G61990	25.773	194	100	7	9	159	216	408	2.73e-12	64.7
MS.gene002752.t1	AT1G61980	26.804	194	98	7	9	159	223	415	3.55e-12	64.3
MS.gene002752.t1	AT1G61980	26.804	194	98	7	9	159	223	415	3.55e-12	64.3
MS.gene002752.t1	AT1G61980	26.804	194	98	7	9	159	223	415	3.55e-12	64.3
MS.gene002752.t1	AT1G61960	30.000	120	78	2	46	159	332	451	1.67e-11	62.4
MS.gene002752.t1	AT5G64950	29.050	179	105	6	1	160	201	376	1.73e-11	62.4

Find 34 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCCTTTCCCATACGGATTT+TGG	0.131006	2.1:-67410166	None:intergenic
TCAATCCAAAGGTTTGATTA+AGG	0.262442	2.1:+67410408	MS.gene002752:CDS
TGAAGAAATTTCAAAACCTT+TGG	0.308581	2.1:-67410388	None:intergenic
AGATGTTTCAACTTTCAATA+AGG	0.340961	2.1:+67410543	MS.gene002752:CDS
AGTATTTGAGAAGGCAGTTT+TGG	0.367653	2.1:+67410075	MS.gene002752:CDS
GATTAATGCGTATAAGAAAT+GGG	0.369032	2.1:+67410189	MS.gene002752:CDS
AGATTAATGCGTATAAGAAA+TGG	0.381339	2.1:+67410188	MS.gene002752:CDS
CATAACAAGCATCGGATGTT+TGG	0.381545	2.1:-67410341	None:intergenic
AGCATCCTTAATCAAACCTT+TGG	0.408692	2.1:-67410413	None:intergenic
TATGGTGAGTTTAGAGAAAA+GGG	0.416619	2.1:+67410360	MS.gene002752:CDS
TTATGGTGAGTTTAGAGAAA+AGG	0.428710	2.1:+67410359	MS.gene002752:CDS
CAAACATCCGATGCTTGTTA+TGG	0.429682	2.1:+67410342	MS.gene002752:CDS
TTTGATTAAGGATGCTAAGT+TGG	0.445791	2.1:+67410420	MS.gene002752:CDS
TCTTAGCATGTAGTGCGATA+AGG	0.479997	2.1:-67410138	None:intergenic
TTGAAATTTCTTCAATCCAA+AGG	0.481678	2.1:+67410397	MS.gene002752:CDS
ATTAATCTTCCTTTCCCATA+CGG	0.515016	2.1:-67410173	None:intergenic
GAGATTTGTGAATTGCTTCG+AGG	0.533006	2.1:+67410480	MS.gene002752:CDS
AGAGAAAAGGGTTATTCCAA+AGG	0.536086	2.1:+67410372	MS.gene002752:CDS
GTCCAAAATCCGTATGGGAA+AGG	0.543559	2.1:+67410164	MS.gene002752:CDS
ACTGCCTTCTCAAATACTGC+AGG	0.547454	2.1:-67410070	None:intergenic
AGATTAGTCCAAAATCCGTA+TGG	0.549136	2.1:+67410158	MS.gene002752:CDS
GAAGTATCCATGGTGTATGT+TGG	0.562557	2.1:+67410246	MS.gene002752:CDS
ATGGTGTATGTTGGCATCTG+TGG	0.563796	2.1:+67410255	MS.gene002752:CDS
CAATCTTCAAAGCAACGCAA+AGG	0.567355	2.1:+67410577	MS.gene002752:CDS
AAACTCACCATAACAAGCAT+CGG	0.567644	2.1:-67410349	None:intergenic
AATCTTCAAAGCAACGCAAA+GGG	0.571909	2.1:+67410578	MS.gene002752:CDS
GATTAGTCCAAAATCCGTAT+GGG	0.594092	2.1:+67410159	MS.gene002752:CDS
ATTAATGCGTATAAGAAATG+GGG	0.599170	2.1:+67410190	MS.gene002752:CDS
GATTCTAAGTTGCAGTGACA+TGG	0.603690	2.1:-67410615	None:intergenic
GAAACCTGCAGTATTTGAGA+AGG	0.606941	2.1:+67410066	MS.gene002752:CDS
ATGCGTATAAGAAATGGGGT+TGG	0.624037	2.1:+67410194	MS.gene002752:CDS
TGCACCTTTCAAAGTCTCTG+AGG	0.644963	2.1:+67410444	MS.gene002752:CDS
ACATCCTCAGAGACTTTGAA+AGG	0.652583	2.1:-67410448	None:intergenic
ACAGATGCCAACATACACCA+TGG	0.670800	2.1:-67410253	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AGATGTTTCAACTTTCAATA+AGG	+	chr2.1:67410543-67410562	MS.gene002752:CDS	25.0%
!	AGATTAATGCGTATAAGAAA+TGG	+	chr2.1:67410188-67410207	MS.gene002752:CDS	25.0%
!	ATAAGGAAAAACGTATTGTT+AGG	-	chr2.1:67410124-67410143	None:intergenic	25.0%
!	ATTAATGCGTATAAGAAATG+GGG	+	chr2.1:67410190-67410209	MS.gene002752:CDS	25.0%
!	GATTAATGCGTATAAGAAAT+GGG	+	chr2.1:67410189-67410208	MS.gene002752:CDS	25.0%
!	TGAAGAAATTTCAAAACCTT+TGG	-	chr2.1:67410391-67410410	None:intergenic	25.0%
!	TTGAAATTTCTTCAATCCAA+AGG	+	chr2.1:67410397-67410416	MS.gene002752:CDS	25.0%
!!!	GTTTTGGAAGTAAAACAATT+AGG	+	chr2.1:67410091-67410110	MS.gene002752:CDS	25.0%
	ATTAATCTTCCTTTCCCATA+CGG	-	chr2.1:67410176-67410195	None:intergenic	30.0%
	TATGGTGAGTTTAGAGAAAA+GGG	+	chr2.1:67410360-67410379	MS.gene002752:CDS	30.0%
	TCAATCCAAAGGTTTGATTA+AGG	+	chr2.1:67410408-67410427	MS.gene002752:CDS	30.0%
	TTATGGTGAGTTTAGAGAAA+AGG	+	chr2.1:67410359-67410378	MS.gene002752:CDS	30.0%
	TTTGATTAAGGATGCTAAGT+TGG	+	chr2.1:67410420-67410439	MS.gene002752:CDS	30.0%
!!!	CAGTTTTTTGTCAATCACAT+GGG	+	chr2.1:67410298-67410317	MS.gene002752:CDS	30.0%
	AAACTCACCATAACAAGCAT+CGG	-	chr2.1:67410352-67410371	None:intergenic	35.0%
	AATCTTCAAAGCAACGCAAA+GGG	+	chr2.1:67410578-67410597	MS.gene002752:CDS	35.0%
	AGAGAAAAGGGTTATTCCAA+AGG	+	chr2.1:67410372-67410391	MS.gene002752:CDS	35.0%
	AGCATCCTTAATCAAACCTT+TGG	-	chr2.1:67410416-67410435	None:intergenic	35.0%
!	AGATTAGTCCAAAATCCGTA+TGG	+	chr2.1:67410158-67410177	MS.gene002752:CDS	35.0%
!	AGTATTTGAGAAGGCAGTTT+TGG	+	chr2.1:67410075-67410094	MS.gene002752:CDS	35.0%
!	GATTAGTCCAAAATCCGTAT+GGG	+	chr2.1:67410159-67410178	MS.gene002752:CDS	35.0%
!	TTTTGTCAATCACATGGGTT+GGG	+	chr2.1:67410303-67410322	MS.gene002752:CDS	35.0%
!	TTTTTGTCAATCACATGGGT+TGG	+	chr2.1:67410302-67410321	MS.gene002752:CDS	35.0%
!!!	CAGCATTTTTGAAGTATCCA+TGG	+	chr2.1:67410236-67410255	MS.gene002752:CDS	35.0%
!!!	GCAGTTTTTTGTCAATCACA+TGG	+	chr2.1:67410297-67410316	MS.gene002752:CDS	35.0%
	ACATCCTCAGAGACTTTGAA+AGG	-	chr2.1:67410451-67410470	None:intergenic	40.0%
	ATGCGTATAAGAAATGGGGT+TGG	+	chr2.1:67410194-67410213	MS.gene002752:CDS	40.0%
	CAATCTTCAAAGCAACGCAA+AGG	+	chr2.1:67410577-67410596	MS.gene002752:CDS	40.0%
	GAAACCTGCAGTATTTGAGA+AGG	+	chr2.1:67410066-67410085	MS.gene002752:CDS	40.0%
	GAGATTTGTGAATTGCTTCG+AGG	+	chr2.1:67410480-67410499	MS.gene002752:CDS	40.0%
	TCTTAGCATGTAGTGCGATA+AGG	-	chr2.1:67410141-67410160	None:intergenic	40.0%
!	CAAACATCCGATGCTTGTTA+TGG	+	chr2.1:67410342-67410361	MS.gene002752:CDS	40.0%
!	GAAGTATCCATGGTGTATGT+TGG	+	chr2.1:67410246-67410265	MS.gene002752:CDS	40.0%
!	TTCCTTTCCCATACGGATTT+TGG	-	chr2.1:67410169-67410188	None:intergenic	40.0%
!!	CATAACAAGCATCGGATGTT+TGG	-	chr2.1:67410344-67410363	None:intergenic	40.0%
	ACAGATGCCAACATACACCA+TGG	-	chr2.1:67410256-67410275	None:intergenic	45.0%
	ACTGCCTTCTCAAATACTGC+AGG	-	chr2.1:67410073-67410092	None:intergenic	45.0%
	ATGGTGTATGTTGGCATCTG+TGG	+	chr2.1:67410255-67410274	MS.gene002752:CDS	45.0%
	GTCCAAAATCCGTATGGGAA+AGG	+	chr2.1:67410164-67410183	MS.gene002752:CDS	45.0%
	TGCACCTTTCAAAGTCTCTG+AGG	+	chr2.1:67410444-67410463	MS.gene002752:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.1	gene	67410055	67410633	67410055	ID=MS.gene002752
chr2.1	mRNA	67410055	67410633	67410055	ID=MS.gene002752.t1;Parent=MS.gene002752
chr2.1	exon	67410055	67410633	67410055	ID=MS.gene002752.t1.exon1;Parent=MS.gene002752.t1
chr2.1	CDS	67410055	67410633	67410055	ID=cds.MS.gene002752.t1;Parent=MS.gene002752.t1

Gene Sequence

>MS.gene002752

ATGCTCTCTAAGAAACCTGCAGTATTTGAGAAGGCAGTTTTGGAAGTAAAACAATTAGGATTCAATCCTAACAATACGTTTTTCCTTATCGCACTACATGCTAAGATTAGTCCAAAATCCGTATGGGAAAGGAAGATTAATGCGTATAAGAAATGGGGTTGGTCTGATGAAGTTATTGTCTCAGCATTTTTGAAGTATCCATGGTGTATGTTGGCATCTGTGGAAAAAATTGAAGCAGTGATGCAGTTTTTTGTCAATCACATGGGTTGGGAGTCTAATGTACTTGCCAAACATCCGATGCTTGTTATGGTGAGTTTAGAGAAAAGGGTTATTCCAAAGGTTTTGAAATTTCTTCAATCCAAAGGTTTGATTAAGGATGCTAAGTTGGCTGCACCTTTCAAAGTCTCTGAGGATGTGTTTTTGAAGAGATTTGTGAATTGCTTCGAGGAAGAAGCATCTCAGCTGTTGAAGTTGTACGAAGAGAAAAGAGATGTTTCAACTTTCAATAAGGTTTCGCAGCTGCAATCTTCAAAGCAACGCAAAGGGTCTCTGCTACACGACCATGTCACTGCAACTTAG

Protein sequence

>MS.gene002752.t1

MLSKKPAVFEKAVLEVKQLGFNPNNTFFLIALHAKISPKSVWERKINAYKKWGWSDEVIVSAFLKYPWCMLASVEKIEAVMQFFVNHMGWESNVLAKHPMLVMVSLEKRVIPKVLKFLQSKGLIKDAKLAAPFKVSEDVFLKRFVNCFEEEASQLLKLYEEKRDVSTFNKVSQLQSSKQRKGSLLHDHVTAT