AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003018

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003018.t1 MTR_2g024430 88.544 515 55 3 1 511 1 515 0.0 938
MS.gene003018.t1 MTR_5g040880 58.949 514 192 9 13 510 4 514 0.0 556
MS.gene003018.t1 MTR_5g082140 53.678 503 182 7 9 505 1 458 5.23e-176 503
MS.gene003018.t1 MTR_5g040740 52.860 507 132 9 9 510 1 405 2.06e-163 469
MS.gene003018.t1 MTR_5g061220 49.815 542 213 13 7 510 6 526 4.92e-162 470
MS.gene003018.t1 MTR_1g058210 45.187 509 198 14 9 503 1 442 3.28e-130 386
MS.gene003018.t1 MTR_2g006270 45.059 506 208 11 13 509 6 450 1.08e-126 377
MS.gene003018.t1 MTR_3g073420 42.576 559 253 13 10 510 9 557 6.19e-123 372
MS.gene003018.t1 MTR_2g006380 41.667 504 219 15 14 509 14 450 1.14e-110 336
MS.gene003018.t1 MTR_5g061890 48.794 373 156 14 9 362 1 357 2.76e-98 302
MS.gene003018.t1 MTR_5g060780 50.142 351 141 13 9 340 1 336 1.33e-97 301
MS.gene003018.t1 MTR_5g460920 50.142 351 141 13 9 340 1 336 4.26e-97 299
MS.gene003018.t1 MTR_5g060630 40.338 533 224 19 9 463 1 517 3.05e-96 301
MS.gene003018.t1 MTR_5g460740 40.338 533 224 19 9 463 1 517 3.05e-96 301
MS.gene003018.t1 MTR_2g094570 39.901 406 169 11 17 361 8 399 5.00e-86 279
MS.gene003018.t1 MTR_2g094570 39.901 406 169 11 17 361 8 399 5.80e-86 279
MS.gene003018.t1 MTR_2g094570 39.474 418 177 12 5 361 53 455 1.15e-85 280
MS.gene003018.t1 MTR_1g064430 39.901 406 175 10 17 360 24 422 2.13e-84 275
MS.gene003018.t1 MTR_1g064430 39.901 406 175 10 17 360 37 435 2.34e-84 275
MS.gene003018.t1 MTR_4g058930 39.078 412 180 10 9 358 1 403 2.34e-83 272
MS.gene003018.t1 MTR_4g058930 39.078 412 180 10 9 358 1 403 4.28e-83 272
MS.gene003018.t1 MTR_1g094960 35.225 511 246 14 17 463 16 505 1.03e-82 269
MS.gene003018.t1 MTR_7g101280 37.019 416 178 12 17 361 28 430 2.99e-75 250
MS.gene003018.t1 MTR_0041s0110 37.019 416 178 12 17 361 28 430 2.99e-75 250
MS.gene003018.t1 MTR_5g062970 53.061 196 86 2 20 213 38 229 2.62e-63 206
MS.gene003018.t1 MTR_7g101275 40.000 320 153 10 30 325 2 306 4.22e-63 211
MS.gene003018.t1 MTR_2g014770 35.696 381 206 12 11 365 45 412 2.51e-58 204
MS.gene003018.t1 MTR_2g014770 35.696 381 206 12 11 365 45 412 6.01e-58 204
MS.gene003018.t1 MTR_0041s0120 38.298 329 158 9 30 325 2 318 1.06e-57 197
MS.gene003018.t1 MTR_4g088210 31.798 456 256 13 20 445 38 468 3.42e-55 197
MS.gene003018.t1 MTR_4g088210 31.798 456 256 13 20 445 38 468 4.66e-55 196
MS.gene003018.t1 MTR_2g093740 31.390 446 242 14 20 415 56 487 4.14e-52 188
MS.gene003018.t1 MTR_2g093740 31.390 446 242 14 20 415 56 487 8.46e-52 188
MS.gene003018.t1 MTR_2g093740 31.390 446 242 14 20 415 56 487 1.45e-51 188
MS.gene003018.t1 MTR_2g005240 35.694 353 195 12 20 350 23 365 1.58e-51 186
MS.gene003018.t1 MTR_8g100050 34.444 360 210 10 20 359 59 412 1.76e-50 184
MS.gene003018.t1 MTR_8g100050 34.444 360 210 10 20 359 59 412 4.88e-50 184
MS.gene003018.t1 MTR_4g021580 32.869 359 213 10 20 358 19 369 3.16e-49 179
MS.gene003018.t1 MTR_4g021580 32.869 359 213 10 20 358 19 369 3.31e-49 180
MS.gene003018.t1 MTR_8g027440 34.722 360 203 12 20 358 29 377 9.14e-49 179
MS.gene003018.t1 MTR_2g093740 30.501 459 242 15 20 415 56 500 1.02e-48 179
MS.gene003018.t1 MTR_2g018690 32.514 366 203 12 11 345 11 363 1.07e-48 180
MS.gene003018.t1 MTR_2g093740 30.501 459 242 15 20 415 56 500 2.37e-48 178
MS.gene003018.t1 MTR_2g093740 30.501 459 242 15 20 415 56 500 2.78e-48 179
MS.gene003018.t1 MTR_7g062540 33.240 358 212 12 20 358 14 363 9.11e-48 176
MS.gene003018.t1 MTR_2g043250 31.268 355 197 11 17 341 20 357 2.68e-47 177
MS.gene003018.t1 MTR_4g060460 31.795 390 238 8 20 383 54 441 3.19e-47 175
MS.gene003018.t1 MTR_4g060460 32.967 364 215 8 20 358 54 413 6.41e-47 174
MS.gene003018.t1 MTR_5g076270 32.386 352 201 10 20 345 24 364 1.18e-46 174
MS.gene003018.t1 MTR_4g124900 31.445 353 195 11 17 339 20 355 1.66e-46 174
MS.gene003018.t1 MTR_4g124900 31.445 353 195 11 17 339 20 355 1.72e-46 174
MS.gene003018.t1 MTR_3g064050 32.102 352 202 10 20 345 26 366 1.49e-45 171
MS.gene003018.t1 MTR_8g079492 32.615 371 195 15 11 345 11 362 1.53e-45 171
MS.gene003018.t1 MTR_8g079492 32.615 371 195 15 11 345 11 362 1.76e-45 171
MS.gene003018.t1 MTR_5g060770 37.536 349 154 14 71 404 8 307 1.84e-45 163
MS.gene003018.t1 MTR_1g024025 34.957 349 183 15 20 339 43 376 2.80e-45 171
MS.gene003018.t1 MTR_8g101360 31.268 355 201 12 17 342 28 368 7.70e-44 166
MS.gene003018.t1 MTR_2g093740 32.615 325 179 9 124 415 40 357 1.29e-40 155
MS.gene003018.t1 MTR_2g093740 32.615 325 179 9 124 415 40 357 2.80e-40 154
MS.gene003018.t1 MTR_8g079492 33.677 291 154 11 81 345 3 280 9.15e-38 148
MS.gene003018.t1 MTR_2g093740 31.361 338 179 10 124 415 40 370 3.25e-37 145
MS.gene003018.t1 MTR_2g093740 31.361 338 179 10 124 415 40 370 4.57e-37 145
MS.gene003018.t1 MTR_5g062960 40.952 210 93 9 238 439 1 187 1.88e-33 126
MS.gene003018.t1 MTR_8g446900 28.114 281 183 6 72 339 14 288 2.19e-29 119
MS.gene003018.t1 MTR_5g460910 31.519 349 153 15 71 404 25 302 7.11e-26 108
MS.gene003018.t1 MTR_5g046120 42.623 122 64 2 52 170 5 123 2.68e-18 81.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003018.t1 AT1G77850 37.316 544 264 17 9 488 1 531 4.98e-99 310
MS.gene003018.t1 AT1G77850 37.316 544 264 17 9 488 1 531 5.16e-99 311
MS.gene003018.t1 AT4G30080 39.906 426 175 14 17 379 16 423 3.08e-82 269
MS.gene003018.t1 AT2G28350 38.462 416 179 12 13 363 3 406 3.90e-79 261
MS.gene003018.t1 AT2G28350 38.462 416 179 12 13 363 3 406 4.42e-79 261
MS.gene003018.t1 AT2G46530 35.616 365 196 15 20 358 21 372 5.36e-54 192
MS.gene003018.t1 AT2G46530 35.616 365 196 15 20 358 42 393 8.16e-54 192
MS.gene003018.t1 AT3G61830 35.882 340 188 13 20 339 25 354 3.29e-53 190
MS.gene003018.t1 AT1G35520 35.509 383 214 14 3 365 10 379 4.41e-52 186
MS.gene003018.t1 AT4G23980 33.628 339 198 9 20 339 12 342 4.25e-51 184
MS.gene003018.t1 AT4G23980 33.628 339 198 9 20 339 12 342 4.93e-51 184
MS.gene003018.t1 AT1G34390 35.638 376 207 16 3 358 10 370 9.90e-51 183
MS.gene003018.t1 AT5G37020 33.523 352 197 10 20 345 22 362 1.06e-50 185
MS.gene003018.t1 AT5G37020 33.523 352 197 10 20 345 22 362 1.27e-50 185
MS.gene003018.t1 AT1G34310 35.000 380 206 14 3 358 10 372 1.70e-50 182
MS.gene003018.t1 AT1G34410 33.943 383 220 13 3 365 10 379 1.98e-50 182
MS.gene003018.t1 AT2G33860 34.493 345 191 10 20 339 54 388 4.85e-50 181
MS.gene003018.t1 AT5G62000 33.705 359 212 10 20 358 61 413 3.92e-49 181
MS.gene003018.t1 AT5G62000 33.705 359 212 10 20 358 61 413 3.92e-49 181
MS.gene003018.t1 AT5G62000 33.705 359 212 10 20 358 61 413 3.92e-49 181
MS.gene003018.t1 AT5G62000 33.705 359 212 10 20 358 61 413 3.92e-49 181
MS.gene003018.t1 AT5G62000 33.705 359 212 10 20 358 61 413 4.52e-49 181
MS.gene003018.t1 AT1G35540 33.898 354 206 12 3 339 10 352 5.50e-49 178
MS.gene003018.t1 AT1G59750 34.211 342 193 11 20 339 22 353 6.27e-49 179
MS.gene003018.t1 AT1G59750 34.211 342 193 11 20 339 22 353 6.75e-49 179
MS.gene003018.t1 AT1G59750 34.211 342 193 11 20 339 22 353 7.88e-49 179
MS.gene003018.t1 AT1G59750 34.211 342 193 11 20 339 22 353 7.88e-49 179
MS.gene003018.t1 AT5G60450 34.595 370 208 13 15 356 36 399 2.73e-48 178
MS.gene003018.t1 AT5G60450 34.795 365 204 13 20 356 65 423 5.99e-48 177
MS.gene003018.t1 AT1G35240 34.225 374 217 13 3 358 10 372 6.04e-48 175
MS.gene003018.t1 AT1G30330 32.603 365 204 11 12 345 10 363 1.15e-47 177
MS.gene003018.t1 AT1G30330 32.425 367 204 11 12 345 10 365 3.03e-47 176
MS.gene003018.t1 AT1G30330 32.425 367 204 11 12 345 10 365 3.03e-47 176
MS.gene003018.t1 AT1G19850 34.262 359 179 12 17 339 40 377 3.77e-46 173
MS.gene003018.t1 AT1G19850 34.262 359 179 12 17 339 51 388 4.80e-46 172
MS.gene003018.t1 AT5G20730 30.878 353 198 10 17 339 21 357 3.91e-44 167
MS.gene003018.t1 AT5G20730 30.704 355 200 10 17 341 21 359 4.36e-44 167
MS.gene003018.t1 AT1G19220 31.461 356 195 11 17 341 20 357 5.62e-44 167
MS.gene003018.t1 AT5G20730 30.704 355 199 11 17 341 21 358 6.88e-43 164
MS.gene003018.t1 AT1G34170 29.487 468 274 18 20 456 25 467 2.46e-42 158
MS.gene003018.t1 AT3G61830 36.842 285 154 11 72 339 28 303 3.45e-42 158
MS.gene003018.t1 AT2G46530 37.638 271 142 11 106 358 24 285 5.57e-40 152
MS.gene003018.t1 AT2G33860 37.931 232 124 7 124 339 4 231 7.47e-40 150
MS.gene003018.t1 AT5G37020 35.685 241 130 8 124 345 33 267 1.81e-37 147
MS.gene003018.t1 AT1G34170 28.758 459 263 18 20 456 25 441 1.42e-36 141
MS.gene003018.t1 AT1G34170 28.667 450 258 17 20 448 25 432 9.70e-36 140
MS.gene003018.t1 AT1G43950 36.522 230 121 7 2 222 9 222 1.35e-31 121
MS.gene003018.t1 AT4G01500 30.597 134 90 1 124 257 38 168 2.95e-11 65.5

Find 138 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGGTTCATTTATGCTATT+TGG 0.105998 2.2:-61623896 MS.gene003018:CDS
AACATTAGCACATCTATATT+TGG 0.209645 2.2:-61624007 MS.gene003018:CDS
TATATATATTATAGGTTAAC+TGG 0.218480 2.2:-61624384 MS.gene003018:intron
GGAGACATGGCGGAAATATT+TGG 0.255210 2.2:+61626302 None:intergenic
CTTCCAACTCTTTCAATGAT+AGG 0.271660 2.2:-61624090 MS.gene003018:CDS
TTCTTTGGTGCTGCACTTGT+GGG 0.284414 2.2:+61623925 None:intergenic
TGAACCAGGATTAACTTCTT+TGG 0.304162 2.2:+61623910 None:intergenic
AGGGATAGGGTTTCAGTGTT+GGG 0.310433 2.2:+61626149 None:intergenic
ACCTCCCAAGGGCTTTGCTT+TGG 0.312781 2.2:+61624337 None:intergenic
GACACCTGATCACCACTAAT+TGG 0.319561 2.2:-61625761 MS.gene003018:CDS
GAGCAGCACCGCCAAATCTT+TGG 0.321198 2.2:+61626257 None:intergenic
CCGACGTTCATGGCAAAGTC+TGG 0.342190 2.2:-61625818 MS.gene003018:CDS
TACAATTGCCCTCAGCAAAA+AGG 0.346652 2.2:-61624282 MS.gene003018:CDS
TTTGGATTATGTGAGACTTC+AGG 0.376866 2.2:+61624355 None:intergenic
AGGATGCAATGAAGATTAAC+TGG 0.377747 2.2:-61625497 MS.gene003018:CDS
AATCTTTGGTTCTACACGGC+TGG 0.380640 2.2:+61626271 None:intergenic
ATCCGTCGGCATACTAAGTT+TGG 0.389012 2.2:-61625651 MS.gene003018:CDS
CTTCTTTGGTGCTGCACTTG+TGG 0.393725 2.2:+61623924 None:intergenic
CCCTTTGAGACAAAGTTTGA+CGG 0.397840 2.2:-61623950 MS.gene003018:CDS
AATATAGATGTGCTAATGTT+TGG 0.410256 2.2:+61624010 None:intergenic
AAAATCCTTGAATACATTGT+TGG 0.413811 2.2:-61624176 MS.gene003018:CDS
CTGTCTGTTACCGACGTTCA+TGG 0.414559 2.2:-61625828 MS.gene003018:CDS
CACCGTTATTAGCATCAGAT+AGG 0.414739 2.2:+61625931 None:intergenic
CCGTCAAACTTTGTCTCAAA+GGG 0.416058 2.2:+61623950 None:intergenic
GGTGATGAAATTGTTTCCTC+TGG 0.421466 2.2:-61625966 MS.gene003018:CDS
AAGATTTGGCGGTGCTGCTC+CGG 0.422842 2.2:-61626254 MS.gene003018:CDS
GGGATAGGGTTTCAGTGTTG+GGG 0.425954 2.2:+61626150 None:intergenic
GCCGTGTAGAACCAAAGATT+TGG 0.431938 2.2:-61626268 MS.gene003018:CDS
TACTCCAATTAGTGGTGATC+AGG 0.433415 2.2:+61625757 None:intergenic
AGCATGCGCCATGGACGATT+AGG 0.434996 2.2:+61625381 None:intergenic
TCATCTGGAGATATGTTTGT+TGG 0.440275 2.2:-61625675 MS.gene003018:CDS
GAAACCCTATCCCTTCTTGA+TGG 0.440854 2.2:-61626140 MS.gene003018:CDS
ACAATCCAACAATGTATTCA+AGG 0.441558 2.2:+61624171 None:intergenic
AACCATCAGTGATGGGAGTT+AGG 0.442469 2.2:+61626033 None:intergenic
CATGTTCTAAGTGACCTTGC+GGG 0.450390 2.2:+61626186 None:intergenic
CAAGGGAGAAGATGGTTGTA+TGG 0.451305 2.2:-61623828 MS.gene003018:CDS
ATCTTTGGTTCTACACGGCT+GGG 0.452340 2.2:+61626272 None:intergenic
CACGAACTTACTCCAATTAG+TGG 0.453246 2.2:+61625749 None:intergenic
GGTTATGGCGGCAGCGGAGT+TGG 0.455795 2.2:-61625604 MS.gene003018:CDS
GAGATGGCTGTTTAGTTTCG+AGG 0.456957 2.2:+61625856 None:intergenic
AAGGGATAGGGTTTCAGTGT+TGG 0.458344 2.2:+61626148 None:intergenic
TCATGCAAATTACTTCCAAC+AGG 0.463742 2.2:+61623860 None:intergenic
TTCAAAATGCTATGGTTAAG+AGG 0.466292 2.2:+61623974 None:intergenic
GATAACTAAAGAATCGGTTA+TGG 0.468357 2.2:-61625619 MS.gene003018:CDS
GGTTCTACACGGCTGGGTTG+TGG 0.468361 2.2:+61626278 None:intergenic
TTTCAATCCTTGCATTGTAT+CGG 0.468436 2.2:-61626108 MS.gene003018:CDS
GAGTATTATCCTACGATTGA+AGG 0.470883 2.2:-61625555 MS.gene003018:CDS
TTCCAACTCTTTCAATGATA+GGG 0.473757 2.2:-61624089 MS.gene003018:CDS
ACAAAGACTTCATCAGTATG+AGG 0.479542 2.2:+61626065 None:intergenic
GATAGGGTTTCAGTGTTGGG+GGG 0.483168 2.2:+61626152 None:intergenic
ACCGTCAAACTTTGTCTCAA+AGG 0.497762 2.2:+61623949 None:intergenic
CATTGCATCCTCAACAACCT+TGG 0.498204 2.2:+61625509 None:intergenic
GGATAGGGTTTCAGTGTTGG+GGG 0.498312 2.2:+61626151 None:intergenic
AGAATCGGTTATGGCGGCAG+CGG 0.500580 2.2:-61625610 MS.gene003018:CDS
GGCCCTATCATTGAAAGAGT+TGG 0.504567 2.2:+61624087 None:intergenic
CTGGAAATCTATCATTGGAA+TGG 0.511209 2.2:+61624210 None:intergenic
TCTGCTACCAAGGGAGAAGA+TGG 0.513250 2.2:-61623836 MS.gene003018:CDS
GAATGCTGGAAATCTATCAT+TGG 0.515653 2.2:+61624205 None:intergenic
GAAATATTTGGTTCTACCAT+CGG 0.518665 2.2:+61626314 None:intergenic
TGCTAAGATAACTAAAGAAT+CGG 0.520496 2.2:-61625625 MS.gene003018:CDS
CGTGACCATCAAGAAGGGAT+AGG 0.523750 2.2:+61626135 None:intergenic
TCTCTCTATATTATCTAATG+AGG 0.525248 2.2:+61624237 None:intergenic
TTTGCATGATTCTGCTACCA+AGG 0.526896 2.2:-61623846 MS.gene003018:CDS
GCTGGGTTGTGGTGGAGACA+TGG 0.530128 2.2:+61626289 None:intergenic
ATATTATAGGTTAACTGGGA+TGG 0.530339 2.2:-61624379 MS.gene003018:intron
AGTGGTGATCAGGTGTCTCA+AGG 0.534349 2.2:+61625767 None:intergenic
TAATCCAAAGCAAAGCCCTT+GGG 0.536094 2.2:-61624341 MS.gene003018:CDS
GTATGGGCTGCAATAATTGA+AGG 0.536513 2.2:-61623811 None:intergenic
GAAATTAGTTCAACCTCCCA+AGG 0.540471 2.2:+61624325 None:intergenic
TATGGCGGCAGCGGAGTTGG+CGG 0.543054 2.2:-61625601 MS.gene003018:CDS
ATGAAGATTAACTGGAATTG+TGG 0.543117 2.2:-61625489 MS.gene003018:CDS
CACTAATTGGAGTAAGTTCG+TGG 0.543836 2.2:-61625748 MS.gene003018:CDS
GATACAATGCAAGGATTGAA+AGG 0.544112 2.2:+61626110 None:intergenic
CAAAGGGGTTCAAAATGCTA+TGG 0.546020 2.2:+61623966 None:intergenic
CTCCTAACTCCCATCACTGA+TGG 0.547018 2.2:-61626035 MS.gene003018:CDS
ATAATCCAAAGCAAAGCCCT+TGG 0.548641 2.2:-61624342 MS.gene003018:CDS
CATACAACCATCTTCTCCCT+TGG 0.552659 2.2:+61623829 None:intergenic
TCGTCCATGGCGCATGCTTG+AGG 0.553392 2.2:-61625376 MS.gene003018:intron
ACCGTTATTAGCATCAGATA+GGG 0.553783 2.2:+61625932 None:intergenic
TCTTCACGAACCTCAGTAGG+AGG 0.555844 2.2:+61625999 None:intergenic
TCTCTGCTCCTAATCGTCCA+TGG 0.557073 2.2:-61625389 MS.gene003018:CDS
TTGCATGATTCTGCTACCAA+GGG 0.558661 2.2:-61623845 MS.gene003018:CDS
TCTTTGGTGCTGCACTTGTG+GGG 0.558880 2.2:+61623926 None:intergenic
ATGATGATTATTCAAAGAGA+TGG 0.559089 2.2:-61625440 MS.gene003018:CDS
GTGTAGAACCAAAGATTTGG+CGG 0.559765 2.2:-61626265 MS.gene003018:CDS
GGCAATTGTAGATTCAGTGC+AGG 0.560065 2.2:+61624295 None:intergenic
ACCCTATCTGATGCTAATAA+CGG 0.564674 2.2:-61625933 MS.gene003018:CDS
TCCTACTGAGGTTCGTGAAG+AGG 0.565181 2.2:-61625997 MS.gene003018:CDS
AGTATTATCCTACGATTGAA+GGG 0.565286 2.2:-61625554 MS.gene003018:CDS
AAGGGAGAAGATGGTTGTAT+GGG 0.567406 2.2:-61623827 MS.gene003018:CDS
GGTGGATGCCAAGGTTGTTG+AGG 0.569678 2.2:-61625517 MS.gene003018:CDS
TCACAAAACCCTTCAATCGT+AGG 0.570407 2.2:+61625546 None:intergenic
GTGAACACAACCATCAGTGA+TGG 0.572216 2.2:+61626025 None:intergenic
CGACGTTCATGGCAAAGTCT+GGG 0.573902 2.2:-61625817 MS.gene003018:CDS
CCAAATAGCATAAATGAACC+AGG 0.581265 2.2:+61623896 None:intergenic
AGCACCAAAGAAGTTAATCC+TGG 0.583231 2.2:-61623914 MS.gene003018:CDS
AAAGTTTACTATTTCCCGCA+AGG 0.585623 2.2:-61626200 MS.gene003018:CDS
GTGACCATCAAGAAGGGATA+GGG 0.586087 2.2:+61626136 None:intergenic
ATATGTTTGTTGGAATCCGT+CGG 0.586941 2.2:-61625665 MS.gene003018:CDS
GTTCGTGAAGAGGAACCTAC+AGG 0.587140 2.2:-61625987 MS.gene003018:CDS
TCTACACGGCTGGGTTGTGG+TGG 0.587866 2.2:+61626281 None:intergenic
GTGGTGATCAGGTGTCTCAA+GGG 0.590017 2.2:+61625768 None:intergenic
TGTTCACAAACCTCCTACTG+AGG 0.591520 2.2:-61626009 MS.gene003018:CDS
AACTAAAGAATCGGTTATGG+CGG 0.592037 2.2:-61625616 MS.gene003018:CDS
GACTTCATCAGTATGAGGAT+CGG 0.593232 2.2:+61626070 None:intergenic
GAAACAATTTCATCACCTGT+AGG 0.594064 2.2:+61625972 None:intergenic
AGGGAGTTCCACGGTAGACA+TGG 0.596664 2.2:+61625787 None:intergenic
ATATATATTATAGGTTAACT+GGG 0.596919 2.2:-61624383 MS.gene003018:intron
CTATCCCTTCTTGATGGTCA+CGG 0.597434 2.2:-61626134 MS.gene003018:CDS
AAGGACCGTGACCATCAAGA+AGG 0.601243 2.2:+61626129 None:intergenic
TGTTTAGTTTCGAGGTCAAG+CGG 0.603245 2.2:+61625864 None:intergenic
GGTAAGATGATACATCCTGT+TGG 0.604159 2.2:-61623875 MS.gene003018:CDS
TAGTTTCGAGGTCAAGCGGT+GGG 0.611231 2.2:+61625868 None:intergenic
TGAACACAACCATCAGTGAT+GGG 0.614197 2.2:+61626026 None:intergenic
GCATGTTCTAAGTGACCTTG+CGG 0.616291 2.2:+61626185 None:intergenic
GCCAAATCTTTGGTTCTACA+CGG 0.618346 2.2:+61626267 None:intergenic
ATTGATGTCGCGCAAGAACG+AGG 0.619010 2.2:+61625894 None:intergenic
TCCTCTTCACGAACCTCAGT+AGG 0.621000 2.2:+61625996 None:intergenic
AGAGTTGGAAGTAGTTCCTG+TGG 0.623129 2.2:+61624102 None:intergenic
TATACCTCAAGCATGCGCCA+TGG 0.631418 2.2:+61625372 None:intergenic
TCCAAAGCAAAGCCCTTGGG+AGG 0.633549 2.2:-61624338 MS.gene003018:CDS
AAATTAGTTCAACCTCCCAA+GGG 0.635245 2.2:+61624326 None:intergenic
CACCAAACTTAGTATGCCGA+CGG 0.637276 2.2:+61625649 None:intergenic
GTAACAGACAGTTCTTGAGA+TGG 0.639997 2.2:+61625840 None:intergenic
AGGACCGTGACCATCAAGAA+GGG 0.643039 2.2:+61626130 None:intergenic
AAAAGGCTTAGAGTTGCTCA+GGG 0.650442 2.2:-61624265 MS.gene003018:CDS
TGTCGCGCAAGAACGAGGTA+CGG 0.658162 2.2:+61625899 None:intergenic
GACGAAAAGAAACTCGTCTG+CGG 0.661354 2.2:-61625726 MS.gene003018:CDS
GGGTTGTGGTGGAGACATGG+CGG 0.664200 2.2:+61626292 None:intergenic
GAGTTTCGCCATGTCTACCG+TGG 0.667413 2.2:-61625795 MS.gene003018:CDS
CGTCAAACTTTGTCTCAAAG+GGG 0.667527 2.2:+61623951 None:intergenic
TCAACGGCCGATACAATGCA+AGG 0.667965 2.2:+61626101 None:intergenic
AGGATCGGCGAGGTAATCAA+CGG 0.669436 2.2:+61626085 None:intergenic
GGTGTCTCAAGGGAGTTCCA+CGG 0.685317 2.2:+61625778 None:intergenic
CCAGACTTTGCCATGAACGT+CGG 0.685939 2.2:+61625818 None:intergenic
CTATCTGATGCTAATAACGG+TGG 0.699278 2.2:-61625930 MS.gene003018:CDS
TTAGTTTCGAGGTCAAGCGG+TGG 0.704813 2.2:+61625867 None:intergenic
CATCAGTATGAGGATCGGCG+AGG 0.728455 2.2:+61626075 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATTATAGGTTAACT+GGG - chr2.2:61625742-61625761 MS.gene003018:CDS 15.0%
!! ATCATATAAATGATTAATGT+TGG - chr2.2:61625695-61625714 MS.gene003018:CDS 15.0%
!! CATCACAATATATATATTAT+AGG - chr2.2:61625733-61625752 MS.gene003018:CDS 15.0%
!! TAAACATATATGTAAACAAA+CGG + chr2.2:61625441-61625460 None:intergenic 15.0%
!! TATATATATTATAGGTTAAC+TGG - chr2.2:61625741-61625760 MS.gene003018:CDS 15.0%
!! AAAAAACAACAACGATATAT+TGG + chr2.2:61625218-61625237 None:intergenic 20.0%
!! AAAAACAACAACGATATATT+GGG + chr2.2:61625217-61625236 None:intergenic 20.0%
!! AATTATTTCAGAAGTTGAAA+TGG - chr2.2:61625397-61625416 MS.gene003018:CDS 20.0%
!! ATTATTTCAGAAGTTGAAAT+GGG - chr2.2:61625398-61625417 MS.gene003018:CDS 20.0%
!! GTTAAAATTAATATCCTACT+TGG + chr2.2:61625616-61625635 None:intergenic 20.0%
!! TAAAATCAATGAAGTTAATG+TGG + chr2.2:61625354-61625373 None:intergenic 20.0%
!! TATGTATCTATATAAGTCAA+TGG + chr2.2:61625664-61625683 None:intergenic 20.0%
!! TTATGAACAATTATATGCAA+AGG - chr2.2:61624942-61624961 MS.gene003018:intron 20.0%
!! TTCATTGAGAAAAATTCAAT+GGG - chr2.2:61626067-61626086 MS.gene003018:CDS 20.0%
!! TTTATGAAAAAGAAATCATC+TGG - chr2.2:61624435-61624454 MS.gene003018:intron 20.0%
!! TTTCATTGAGAAAAATTCAA+TGG - chr2.2:61626066-61626085 MS.gene003018:CDS 20.0%
!!! ATTTTTCTCAATGAAATCAT+TGG + chr2.2:61626062-61626081 None:intergenic 20.0%
!!! TAAACTTTTGAATGAAGTTT+AGG + chr2.2:61623913-61623932 None:intergenic 20.0%
!!! TTTCTAAAAAAACAAGCAAA+TGG + chr2.2:61625264-61625283 None:intergenic 20.0%
! AAAATCCTTGAATACATTGT+TGG - chr2.2:61625949-61625968 MS.gene003018:CDS 25.0%
! AAATACTCTTAATTCCAAGT+AGG - chr2.2:61625599-61625618 MS.gene003018:CDS 25.0%
! AACATTAGCACATCTATATT+TGG - chr2.2:61626118-61626137 MS.gene003018:CDS 25.0%
! AATATAGATGTGCTAATGTT+TGG + chr2.2:61626118-61626137 None:intergenic 25.0%
! AATTAAAACTGATACATCCT+TGG + chr2.2:61625546-61625565 None:intergenic 25.0%
! ACAATTATATGCAAAGGATA+TGG - chr2.2:61624948-61624967 MS.gene003018:intron 25.0%
! ATGATGATTATTCAAAGAGA+TGG - chr2.2:61624685-61624704 MS.gene003018:intron 25.0%
! ATGTGATAAACGTGATAAAT+TGG - chr2.2:61625129-61625148 MS.gene003018:intron 25.0%
! ATTAAAACTGATACATCCTT+GGG + chr2.2:61625545-61625564 None:intergenic 25.0%
! GAAACTTTATACACTATTGA+TGG - chr2.2:61625374-61625393 MS.gene003018:intron 25.0%
! TAAATACCTAATACATCCAT+AGG + chr2.2:61625038-61625057 None:intergenic 25.0%
! TCTCTCTATATTATCTAATG+AGG + chr2.2:61625891-61625910 None:intergenic 25.0%
! TGCTAAGATAACTAAAGAAT+CGG - chr2.2:61624500-61624519 MS.gene003018:intron 25.0%
! TGGATGTATTAGGTATTTAT+AGG - chr2.2:61625039-61625058 MS.gene003018:intron 25.0%
!! GATTGTGATACTTTTATTGA+GGG - chr2.2:61625971-61625990 MS.gene003018:CDS 25.0%
!! TTTTTATGAATTGAATGCCT+AGG + chr2.2:61625640-61625659 None:intergenic 25.0%
!!! GGAATTAAGAGTATTTACAA+TGG + chr2.2:61625595-61625614 None:intergenic 25.0%
!!! GGATATTAATTTTAACTCCT+AGG - chr2.2:61625620-61625639 MS.gene003018:CDS 25.0%
ACAATCCAACAATGTATTCA+AGG + chr2.2:61625957-61625976 None:intergenic 30.0%
AGTATTATCCTACGATTGAA+GGG - chr2.2:61624571-61624590 MS.gene003018:intron 30.0%
ATATTATAGGTTAACTGGGA+TGG - chr2.2:61625746-61625765 MS.gene003018:CDS 30.0%
ATGAAGATTAACTGGAATTG+TGG - chr2.2:61624636-61624655 MS.gene003018:intron 30.0%
GATAACTAAAGAATCGGTTA+TGG - chr2.2:61624506-61624525 MS.gene003018:intron 30.0%
GTACTATTGAAGATGCTTAA+AGG + chr2.2:61625293-61625312 None:intergenic 30.0%
TGTGGTATGACTCATAAAAA+AGG + chr2.2:61625336-61625355 None:intergenic 30.0%
TTCAAAATGCTATGGTTAAG+AGG + chr2.2:61626154-61626173 None:intergenic 30.0%
TTCCAACTCTTTCAATGATA+GGG - chr2.2:61626036-61626055 MS.gene003018:CDS 30.0%
TTGAGAAAAATTCAATGGGA+AGG - chr2.2:61626071-61626090 MS.gene003018:CDS 30.0%
TTTCAATCCTTGCATTGTAT+CGG - chr2.2:61624017-61624036 MS.gene003018:CDS 30.0%
! AAAGAGATGGTCAGTTTTTA+AGG - chr2.2:61624698-61624717 MS.gene003018:intron 30.0%
! AAGAGATGGTCAGTTTTTAA+GGG - chr2.2:61624699-61624718 MS.gene003018:intron 30.0%
! AATCTATGCTCAAGTGATTT+TGG - chr2.2:61624984-61625003 MS.gene003018:intron 30.0%
! ATCTATGCTCAAGTGATTTT+GGG - chr2.2:61624985-61625004 MS.gene003018:intron 30.0%
! GGATTGTGATACTTTTATTG+AGG - chr2.2:61625970-61625989 MS.gene003018:CDS 30.0%
! TTAGTAGCTTTGCAATCTAT+AGG - chr2.2:61624891-61624910 MS.gene003018:intron 30.0%
AAAGTTTACTATTTCCCGCA+AGG - chr2.2:61623925-61623944 MS.gene003018:CDS 35.0%
AAATTAGTTCAACCTCCCAA+GGG + chr2.2:61625802-61625821 None:intergenic 35.0%
AACCACGCATCATCAAATTA+TGG + chr2.2:61624864-61624883 None:intergenic 35.0%
AACTAAAGAATCGGTTATGG+CGG - chr2.2:61624509-61624528 MS.gene003018:intron 35.0%
ACAAAGACTTCATCAGTATG+AGG + chr2.2:61624063-61624082 None:intergenic 35.0%
ACCCTATCTGATGCTAATAA+CGG - chr2.2:61624192-61624211 MS.gene003018:CDS 35.0%
ACCGTTATTAGCATCAGATA+GGG + chr2.2:61624196-61624215 None:intergenic 35.0%
AGGATGCAATGAAGATTAAC+TGG - chr2.2:61624628-61624647 MS.gene003018:intron 35.0%
CAAGCAAATGGATAGAAGTA+TGG + chr2.2:61625252-61625271 None:intergenic 35.0%
CCAAATAGCATAAATGAACC+AGG + chr2.2:61626232-61626251 None:intergenic 35.0%
CCTGGTTCATTTATGCTATT+TGG - chr2.2:61626229-61626248 MS.gene003018:CDS 35.0%
CTATTGAAGATGCTTAAAGG+AGG + chr2.2:61625290-61625309 None:intergenic 35.0%
CTGGAAATCTATCATTGGAA+TGG + chr2.2:61625918-61625937 None:intergenic 35.0%
CTTCCAACTCTTTCAATGAT+AGG - chr2.2:61626035-61626054 MS.gene003018:CDS 35.0%
GAAAAATTCAATGGGAAGGT+TGG - chr2.2:61626075-61626094 MS.gene003018:CDS 35.0%
GAAACAATTTCATCACCTGT+AGG + chr2.2:61624156-61624175 None:intergenic 35.0%
GAATGCTGGAAATCTATCAT+TGG + chr2.2:61625923-61625942 None:intergenic 35.0%
GAGAAGTTGAATGAATGTTG+TGG - chr2.2:61625180-61625199 MS.gene003018:intron 35.0%
GAGTATTATCCTACGATTGA+AGG - chr2.2:61624570-61624589 MS.gene003018:intron 35.0%
GATACAATGCAAGGATTGAA+AGG + chr2.2:61624018-61624037 None:intergenic 35.0%
TCATCTGGAGATATGTTTGT+TGG - chr2.2:61624450-61624469 MS.gene003018:intron 35.0%
TCATGCAAATTACTTCCAAC+AGG + chr2.2:61626268-61626287 None:intergenic 35.0%
TGAACCAGGATTAACTTCTT+TGG + chr2.2:61626218-61626237 None:intergenic 35.0%
TGTGGAAAAAAGACTGTCTT+GGG + chr2.2:61626008-61626027 None:intergenic 35.0%
TTGGAGCAAGTTAAGTTTGT+AGG + chr2.2:61625517-61625536 None:intergenic 35.0%
TTTGGATTATGTGAGACTTC+AGG + chr2.2:61625773-61625792 None:intergenic 35.0%
! ATATGTTTGTTGGAATCCGT+CGG - chr2.2:61624460-61624479 MS.gene003018:intron 35.0%
!! TGAAGGGTTTTGTGATTTTG+TGG - chr2.2:61624587-61624606 MS.gene003018:intron 35.0%
!! TTCCATAATTTGATGATGCG+TGG - chr2.2:61624859-61624878 MS.gene003018:intron 35.0%
AAAAAGGCTTAGAGTTGCTC+AGG - chr2.2:61625859-61625878 MS.gene003018:CDS 40.0%
AAAAGGCTTAGAGTTGCTCA+GGG - chr2.2:61625860-61625879 MS.gene003018:CDS 40.0%
AAGGGAGAAGATGGTTGTAT+GGG - chr2.2:61626298-61626317 MS.gene003018:CDS 40.0%
ACCGTCAAACTTTGTCTCAA+AGG + chr2.2:61626179-61626198 None:intergenic 40.0%
AGCACCAAAGAAGTTAATCC+TGG - chr2.2:61626211-61626230 MS.gene003018:CDS 40.0%
ATAATCCAAAGCAAAGCCCT+TGG - chr2.2:61625783-61625802 MS.gene003018:CDS 40.0%
CAAAGGGGTTCAAAATGCTA+TGG + chr2.2:61626162-61626181 None:intergenic 40.0%
CACCGTTATTAGCATCAGAT+AGG + chr2.2:61624197-61624216 None:intergenic 40.0%
CACGAACTTACTCCAATTAG+TGG + chr2.2:61624379-61624398 None:intergenic 40.0%
CACTAATTGGAGTAAGTTCG+TGG - chr2.2:61624377-61624396 MS.gene003018:intron 40.0%
CCGTCAAACTTTGTCTCAAA+GGG + chr2.2:61626178-61626197 None:intergenic 40.0%
CGTCAAACTTTGTCTCAAAG+GGG + chr2.2:61626177-61626196 None:intergenic 40.0%
CTATCTGATGCTAATAACGG+TGG - chr2.2:61624195-61624214 MS.gene003018:CDS 40.0%
CTGTGGAAAAAAGACTGTCT+TGG + chr2.2:61626009-61626028 None:intergenic 40.0%
GAAATTAGTTCAACCTCCCA+AGG + chr2.2:61625803-61625822 None:intergenic 40.0%
GACTTCATCAGTATGAGGAT+CGG + chr2.2:61624058-61624077 None:intergenic 40.0%
GCTTCTCCTATGGATGTATT+AGG - chr2.2:61625029-61625048 MS.gene003018:intron 40.0%
GGTAAGATGATACATCCTGT+TGG - chr2.2:61626250-61626269 MS.gene003018:CDS 40.0%
GGTGATGAAATTGTTTCCTC+TGG - chr2.2:61624159-61624178 MS.gene003018:CDS 40.0%
GTAACAGACAGTTCTTGAGA+TGG + chr2.2:61624288-61624307 None:intergenic 40.0%
GTGTAGAACCAAAGATTTGG+CGG - chr2.2:61623860-61623879 MS.gene003018:CDS 40.0%
TAATCCAAAGCAAAGCCCTT+GGG - chr2.2:61625784-61625803 MS.gene003018:CDS 40.0%
TACAATTGCCCTCAGCAAAA+AGG - chr2.2:61625843-61625862 MS.gene003018:CDS 40.0%
TACTCCAATTAGTGGTGATC+AGG + chr2.2:61624371-61624390 None:intergenic 40.0%
TCACAAAACCCTTCAATCGT+AGG + chr2.2:61624582-61624601 None:intergenic 40.0%
TGAACACAACCATCAGTGAT+GGG + chr2.2:61624102-61624121 None:intergenic 40.0%
TTGCATGATTCTGCTACCAA+GGG - chr2.2:61626280-61626299 MS.gene003018:CDS 40.0%
TTTGCATGATTCTGCTACCA+AGG - chr2.2:61626279-61626298 MS.gene003018:CDS 40.0%
! AACTCTAAGCCTTTTTGCTG+AGG + chr2.2:61625855-61625874 None:intergenic 40.0%
! ACTCTAAGCCTTTTTGCTGA+GGG + chr2.2:61625854-61625873 None:intergenic 40.0%
! GATACATCCTTGGGAACTTT+TGG + chr2.2:61625536-61625555 None:intergenic 40.0%
! TAGGGTTAGAGTTTTTCCAG+AGG + chr2.2:61624178-61624197 None:intergenic 40.0%
! TGTTTAGTTTCGAGGTCAAG+CGG + chr2.2:61624264-61624283 None:intergenic 40.0%
!! AGGAATTTTGACAGAGTGTC+CGG + chr2.2:61623893-61623912 None:intergenic 40.0%
!! AGGGTTTTGTGATTTTGTGG+TGG - chr2.2:61624590-61624609 MS.gene003018:intron 40.0%
!! CCCTTTGAGACAAAGTTTGA+CGG - chr2.2:61626175-61626194 MS.gene003018:CDS 40.0%
!! GCCAAATCTTTGGTTCTACA+CGG + chr2.2:61623861-61623880 None:intergenic 40.0%
!!! AGGATTTTGCTGTTGAATGC+TGG + chr2.2:61625937-61625956 None:intergenic 40.0%
!!! CAAGACAGTCTTTTTTCCAC+AGG - chr2.2:61626007-61626026 MS.gene003018:CDS 40.0%
AAGGGATAGGGTTTCAGTGT+TGG + chr2.2:61623980-61623999 None:intergenic 45.0%
ACTTGCTCCAAAAGTTCCCA+AGG - chr2.2:61625526-61625545 MS.gene003018:CDS 45.0%
AGAGTTGGAAGTAGTTCCTG+TGG + chr2.2:61626026-61626045 None:intergenic 45.0%
AGGGATAGGGTTTCAGTGTT+GGG + chr2.2:61623979-61623998 None:intergenic 45.0%
ATCCGTCGGCATACTAAGTT+TGG - chr2.2:61624474-61624493 MS.gene003018:intron 45.0%
CAAACGGTGAGACAACTTCT+TGG + chr2.2:61625425-61625444 None:intergenic 45.0%
CAACGAAGATGCTTCTCCTA+TGG - chr2.2:61625019-61625038 MS.gene003018:intron 45.0%
CAAGGGAGAAGATGGTTGTA+TGG - chr2.2:61626297-61626316 MS.gene003018:CDS 45.0%
CACCAAACTTAGTATGCCGA+CGG + chr2.2:61624479-61624498 None:intergenic 45.0%
CATACAACCATCTTCTCCCT+TGG + chr2.2:61626299-61626318 None:intergenic 45.0%
CATTGCATCCTCAACAACCT+TGG + chr2.2:61624619-61624638 None:intergenic 45.0%
GAAACCCTATCCCTTCTTGA+TGG - chr2.2:61623985-61624004 MS.gene003018:CDS 45.0%
GACACCTGATCACCACTAAT+TGG - chr2.2:61624364-61624383 MS.gene003018:CDS 45.0%
GACGAAAAGAAACTCGTCTG+CGG - chr2.2:61624399-61624418 MS.gene003018:intron 45.0%
GCCGTGTAGAACCAAAGATT+TGG - chr2.2:61623857-61623876 MS.gene003018:CDS 45.0%
GGCAATTGTAGATTCAGTGC+AGG + chr2.2:61625833-61625852 None:intergenic 45.0%
GGCCCTATCATTGAAAGAGT+TGG + chr2.2:61626041-61626060 None:intergenic 45.0%
GTGAACACAACCATCAGTGA+TGG + chr2.2:61624103-61624122 None:intergenic 45.0%
GTGACCATCAAGAAGGGATA+GGG + chr2.2:61623992-61624011 None:intergenic 45.0%
TGTTCACAAACCTCCTACTG+AGG - chr2.2:61624116-61624135 MS.gene003018:CDS 45.0%
! AACCATCAGTGATGGGAGTT+AGG + chr2.2:61624095-61624114 None:intergenic 45.0%
! CATGTTCTAAGTGACCTTGC+GGG + chr2.2:61623942-61623961 None:intergenic 45.0%
! CTATCCCTTCTTGATGGTCA+CGG - chr2.2:61623991-61624010 MS.gene003018:CDS 45.0%
! GAGATGGCTGTTTAGTTTCG+AGG + chr2.2:61624272-61624291 None:intergenic 45.0%
! GCATGTTCTAAGTGACCTTG+CGG + chr2.2:61623943-61623962 None:intergenic 45.0%
! GGAGACATGGCGGAAATATT+TGG + chr2.2:61623826-61623845 None:intergenic 45.0%
! TTTCAGTGTTGGGGGGATTA+GGG + chr2.2:61623969-61623988 None:intergenic 45.0%
!! AATCTTTGGTTCTACACGGC+TGG + chr2.2:61623857-61623876 None:intergenic 45.0%
!! ATCTTTGGTTCTACACGGCT+GGG + chr2.2:61623856-61623875 None:intergenic 45.0%
!! CATAGGAGAAGCATCTTCGT+TGG + chr2.2:61625021-61625040 None:intergenic 45.0%
!! TTCTTTGGTGCTGCACTTGT+GGG + chr2.2:61626203-61626222 None:intergenic 45.0%
!!! TGATTTTGTGGTGGATGCCA+AGG - chr2.2:61624599-61624618 MS.gene003018:intron 45.0%
AAGGACCGTGACCATCAAGA+AGG + chr2.2:61623999-61624018 None:intergenic 50.0%
AGGACCGTGACCATCAAGAA+GGG + chr2.2:61623998-61624017 None:intergenic 50.0%
AGGATCGGCGAGGTAATCAA+CGG + chr2.2:61624043-61624062 None:intergenic 50.0%
AGTGGTGATCAGGTGTCTCA+AGG + chr2.2:61624361-61624380 None:intergenic 50.0%
ATTGATGTCGCGCAAGAACG+AGG + chr2.2:61624234-61624253 None:intergenic 50.0%
CCAGACTTTGCCATGAACGT+CGG + chr2.2:61624310-61624329 None:intergenic 50.0%
CGACGTTCATGGCAAAGTCT+GGG - chr2.2:61624308-61624327 MS.gene003018:CDS 50.0%
CGTGACCATCAAGAAGGGAT+AGG + chr2.2:61623993-61624012 None:intergenic 50.0%
CTCCTAACTCCCATCACTGA+TGG - chr2.2:61624090-61624109 MS.gene003018:CDS 50.0%
CTGTCTGTTACCGACGTTCA+TGG - chr2.2:61624297-61624316 MS.gene003018:CDS 50.0%
GGGATAGGGTTTCAGTGTTG+GGG + chr2.2:61623978-61623997 None:intergenic 50.0%
GTGGTGATCAGGTGTCTCAA+GGG + chr2.2:61624360-61624379 None:intergenic 50.0%
GTTCGTGAAGAGGAACCTAC+AGG - chr2.2:61624138-61624157 MS.gene003018:CDS 50.0%
TAGTTTCGAGGTCAAGCGGT+GGG + chr2.2:61624260-61624279 None:intergenic 50.0%
TATACCTCAAGCATGCGCCA+TGG + chr2.2:61624756-61624775 None:intergenic 50.0%
TCAACGGCCGATACAATGCA+AGG + chr2.2:61624027-61624046 None:intergenic 50.0%
TCCTACTGAGGTTCGTGAAG+AGG - chr2.2:61624128-61624147 MS.gene003018:CDS 50.0%
TCCTCTTCACGAACCTCAGT+AGG + chr2.2:61624132-61624151 None:intergenic 50.0%
TCTCTGCTCCTAATCGTCCA+TGG - chr2.2:61624736-61624755 MS.gene003018:intron 50.0%
TCTGCTACCAAGGGAGAAGA+TGG - chr2.2:61626289-61626308 MS.gene003018:CDS 50.0%
TCTTCACGAACCTCAGTAGG+AGG + chr2.2:61624129-61624148 None:intergenic 50.0%
TTAGTTTCGAGGTCAAGCGG+TGG + chr2.2:61624261-61624280 None:intergenic 50.0%
! GATAGGGTTTCAGTGTTGGG+GGG + chr2.2:61623976-61623995 None:intergenic 50.0%
! GGATAGGGTTTCAGTGTTGG+GGG + chr2.2:61623977-61623996 None:intergenic 50.0%
! GTTTCAGTGTTGGGGGGATT+AGG + chr2.2:61623970-61623989 None:intergenic 50.0%
!! CTTCTTTGGTGCTGCACTTG+TGG + chr2.2:61626204-61626223 None:intergenic 50.0%
!! TCTTTGGTGCTGCACTTGTG+GGG + chr2.2:61626202-61626221 None:intergenic 50.0%
ACCTCCCAAGGGCTTTGCTT+TGG + chr2.2:61625791-61625810 None:intergenic 55.0%
AGAATCGGTTATGGCGGCAG+CGG - chr2.2:61624515-61624534 MS.gene003018:intron 55.0%
AGCATGCGCCATGGACGATT+AGG + chr2.2:61624747-61624766 None:intergenic 55.0%
AGGGAGTTCCACGGTAGACA+TGG + chr2.2:61624341-61624360 None:intergenic 55.0%
CATCAGTATGAGGATCGGCG+AGG + chr2.2:61624053-61624072 None:intergenic 55.0%
CCGACGTTCATGGCAAAGTC+TGG - chr2.2:61624307-61624326 MS.gene003018:CDS 55.0%
GAGCAGCACCGCCAAATCTT+TGG + chr2.2:61623871-61623890 None:intergenic 55.0%
GAGTTTCGCCATGTCTACCG+TGG - chr2.2:61624330-61624349 MS.gene003018:CDS 55.0%
GGTGGATGCCAAGGTTGTTG+AGG - chr2.2:61624608-61624627 MS.gene003018:intron 55.0%
GGTGTCTCAAGGGAGTTCCA+CGG + chr2.2:61624350-61624369 None:intergenic 55.0%
TCCAAAGCAAAGCCCTTGGG+AGG - chr2.2:61625787-61625806 MS.gene003018:CDS 55.0%
TGTCGCGCAAGAACGAGGTA+CGG + chr2.2:61624229-61624248 None:intergenic 55.0%
!! AAGATTTGGCGGTGCTGCTC+CGG - chr2.2:61623871-61623890 MS.gene003018:CDS 55.0%
GGTTCTACACGGCTGGGTTG+TGG + chr2.2:61623850-61623869 None:intergenic 60.0%
TCGTCCATGGCGCATGCTTG+AGG - chr2.2:61624749-61624768 MS.gene003018:intron 60.0%
TCTACACGGCTGGGTTGTGG+TGG + chr2.2:61623847-61623866 None:intergenic 60.0%
! GCTGGGTTGTGGTGGAGACA+TGG + chr2.2:61623839-61623858 None:intergenic 60.0%
! GGGTTGTGGTGGAGACATGG+CGG + chr2.2:61623836-61623855 None:intergenic 60.0%
GGTTATGGCGGCAGCGGAGT+TGG - chr2.2:61624521-61624540 MS.gene003018:intron 65.0%
TATGGCGGCAGCGGAGTTGG+CGG - chr2.2:61624524-61624543 MS.gene003018:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 61623814 61626333 61623814 ID=MS.gene003018
chr2.2 mRNA 61623814 61626333 61623814 ID=MS.gene003018.t1;Parent=MS.gene003018
chr2.2 exon 61625377 61626333 61625377 ID=MS.gene003018.t1.exon1;Parent=MS.gene003018.t1
chr2.2 CDS 61625377 61626333 61625377 ID=cds.MS.gene003018.t1;Parent=MS.gene003018.t1
chr2.2 exon 61623814 61624392 61623814 ID=MS.gene003018.t1.exon2;Parent=MS.gene003018.t1
chr2.2 CDS 61623814 61624392 61623814 ID=cds.MS.gene003018.t1;Parent=MS.gene003018.t1
Gene Sequence

>MS.gene003018

ATGGTAGAACCAAATATTTCCGCCATGTCTCCACCACAACCCAGCCGTGTAGAACCAAAGATTTGGCGGTGCTGCTCCGGACACTCTGTCAAAATTCCTAAACTTCATTCAAAAGTTTACTATTTCCCGCAAGGTCACTTAGAACATGCTTGCCCTAATCCCCCCAACACTGAAACCCTATCCCTTCTTGATGGTCACGGTCCTTTCAATCCTTGCATTGTATCGGCCGTTGATTACCTCGCCGATCCTCATACTGATGAAGTCTTTGTCAAACTTCTCCTAACTCCCATCACTGATGGTTGTGTTCACAAACCTCCTACTGAGGTTCGTGAAGAGGAACCTACAGGTGATGAAATTGTTTCCTCTGGAAAAACTCTAACCCTATCTGATGCTAATAACGGTGGTGCATTCTCCGTACCTCGTTCTTGCGCGACATCAATCTTCCCACCGCTTGACCTCGAAACTAAACAGCCATCTCAAGAACTGTCTGTTACCGACGTTCATGGCAAAGTCTGGGAGTTTCGCCATGTCTACCGTGGAACTCCCTTGAGACACCTGATCACCACTAATTGGAGTAAGTTCGTGGACGAAAAGAAACTCGTCTGCGGCGATTCTGTAATTTTTATGAAAAAGAAATCATCTGGAGATATGTTTGTTGGAATCCGTCGGCATACTAAGTTTGGTGCTGCTAAGATAACTAAAGAATCGGTTATGGCGGCAGCGGAGTTGGCGGATAAGAATATCGCTTTTGAAGTTGAGTATTATCCTACGATTGAAGGGTTTTGTGATTTTGTGGTGGATGCCAAGGTTGTTGAGGATGCAATGAAGATTAACTGGAATTGTGGAATGAGAATTAAATTTTCTTTGAAGAATGATGATTATTCAAAGAGATGGTCAGTTTTTAAGGGTACAATATCTGCTCTCTCTGCTCCTAATCGTCCATGGCGCATGCTTGAGGTATATTTATATTGATCATCTTTTGTATTATATGCAACATTTTTGTTTCATTAATGTTATTACAATTGAATTTGATATAAACGTGATTTTCCATAATTTGATGATGCGTGGTTCATGAAATTAGTAGCTTTGCAATCTATAGGATAAGTTACTATGTGATTCTTTGTGAAATTATGAACAATTATATGCAAAGGATATGGAGTGTATATAGAAAATCTATGCTCAAGTGATTTTGGGCATTTAATAACCAACGAAGATGCTTCTCCTATGGATGTATTAGGTATTTATAGGAAAATGTTTTTCAGACACGCATAAACTCATTAAACAATTTGAGAGAGAAAGAAATAAAAAGAGTTATATGTGATAAACGTGATAAATTGGTAATGTGATAAGAATAGAGAGATAAAATGAGAAGTTGAATGAATGTTGTGGAATTAAGTTGTCCCAATATATCGTTGTTGTTTTTTATTTTATATTACCATACTTCTATCCATTTGCTTGTTTTTTTAGAAACATCCTCCTTTAAGCATCTTCAATAGTACAATTAAATTAGAGTTGCTTTCCTTTTTTATGAGTCATACCACATTAACTTCATTGATTTTAGAAACTTTATACACTATTGATGGAATTATTTCAGAAGTTGAAATGGGTCCAAGAAGTTGTCTCACCGTTTGTTTACATATATGTTTAGATTACATTTTAATAATGTTTAGACAATGTTATCACAACGATTTTTACAAGTACCTACAAACTTAACTTGCTCCAAAAGTTCCCAAGGATGTATCAGTTTTAATTAAGTTTAAGAACTTTTTGAGTAACTTCCATTGTAAATACTCTTAATTCCAAGTAGGATATTAATTTTAACTCCTAGGCATTCAATTCATAAAAAACCATTGACTTATATAGATACATACTATATTTCATATCATATAAATGATTAATGTTGGATTCTCACAATTTTTCATCACAATATATATATTATAGGTTAACTGGGATGGACCTGAAGTCTCACATAATCCAAAGCAAAGCCCTTGGGAGGTTGAACTAATTTCTTATATACCTGCACTGAATCTACAATTGCCCTCAGCAAAAAGGCTTAGAGTTGCTCAGGGTTATACCTCATTAGATAATATAGAGAGATCTTCCATTCCAATGATAGATTTCCAGCATTCAACAGCAAAATCCTTGAATACATTGTTGGATTGTGATACTTTTATTGAGGGCATGCAGAGAGCCCAAGACAGTCTTTTTTCCACAGGAACTACTTCCAACTCTTTCAATGATAGGGCCAATGATTTCATTGAGAAAAATTCAATGGGAAGGTTGGATATTGTGTCAACTGAGCCAAACATTAGCACATCTATATTTGGTGACTTGTCACCTCTTAACCATAGCATTTTGAACCCCTTTGAGACAAAGTTTGACGGTCCCCACAAGTGCAGCACCAAAGAAGTTAATCCTGGTTCATTTATGCTATTTGGTAAGATGATACATCCTGTTGGAAGTAATTTGCATGATTCTGCTACCAAGGGAGAAGATGGTTGTATGGGCTGCAATAATTGA
Protein sequence

>MS.gene003018.t1

MVEPNISAMSPPQPSRVEPKIWRCCSGHSVKIPKLHSKVYYFPQGHLEHACPNPPNTETLSLLDGHGPFNPCIVSAVDYLADPHTDEVFVKLLLTPITDGCVHKPPTEVREEEPTGDEIVSSGKTLTLSDANNGGAFSVPRSCATSIFPPLDLETKQPSQELSVTDVHGKVWEFRHVYRGTPLRHLITTNWSKFVDEKKLVCGDSVIFMKKKSSGDMFVGIRRHTKFGAAKITKESVMAAAELADKNIAFEVEYYPTIEGFCDFVVDAKVVEDAMKINWNCGMRIKFSLKNDDYSKRWSVFKGTISALSAPNRPWRMLEVNWDGPEVSHNPKQSPWEVELISYIPALNLQLPSAKRLRVAQGYTSLDNIERSSIPMIDFQHSTAKSLNTLLDCDTFIEGMQRAQDSLFSTGTTSNSFNDRANDFIEKNSMGRLDIVSTEPNISTSIFGDLSPLNHSILNPFETKFDGPHKCSTKEVNPGSFMLFGKMIHPVGSNLHDSATKGEDGCMGCNN