AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003101

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003101.t1 MTR_2g026250 90.516 601 49 4 1 596 1 598 0.0 1114
MS.gene003101.t1 MTR_4g133660 55.132 604 202 8 1 595 1 544 0.0 622
MS.gene003101.t1 MTR_5g097480 47.769 605 244 13 1 594 1 544 0.0 530
MS.gene003101.t1 MTR_3g056110 54.588 425 189 3 173 594 119 542 6.10e-170 494
MS.gene003101.t1 MTR_5g094450 56.959 388 162 3 211 594 146 532 8.56e-160 468
MS.gene003101.t1 MTR_2g082090 54.712 382 166 2 215 593 201 578 6.21e-148 439
MS.gene003101.t1 MTR_1g029420 47.170 371 195 1 224 594 223 592 4.83e-127 386
MS.gene003101.t1 MTR_3g089055 47.170 371 195 1 224 594 201 570 4.42e-123 375
MS.gene003101.t1 MTR_3g089055 47.170 371 195 1 224 594 196 565 5.55e-123 375
MS.gene003101.t1 MTR_7g027190 34.343 396 223 6 221 595 295 674 1.76e-70 240
MS.gene003101.t1 MTR_4g064160 32.540 378 245 3 221 593 311 683 1.43e-64 224
MS.gene003101.t1 MTR_4g064150 30.688 378 256 4 221 593 356 732 1.52e-62 220
MS.gene003101.t1 MTR_4g064200 32.540 378 243 6 221 593 279 649 6.83e-62 216
MS.gene003101.t1 MTR_7g062120 30.133 375 260 2 221 594 367 740 5.06e-61 216
MS.gene003101.t1 MTR_2g097390 31.117 376 251 4 221 593 270 640 1.05e-59 210
MS.gene003101.t1 MTR_2g097310 32.361 377 245 6 221 593 267 637 1.05e-59 210
MS.gene003101.t1 MTR_6g047750 33.333 393 217 9 224 594 293 662 1.42e-58 207
MS.gene003101.t1 MTR_7g057230 33.511 376 233 6 224 593 292 656 1.69e-58 207
MS.gene003101.t1 MTR_2g097463 29.947 374 257 3 221 593 285 654 7.38e-57 202
MS.gene003101.t1 MTR_2g097467 29.708 377 260 4 218 593 281 653 9.83e-57 202
MS.gene003101.t1 MTR_2g097350 31.746 378 246 6 221 593 269 639 1.46e-56 202
MS.gene003101.t1 MTR_2g097473 30.933 375 252 6 221 593 284 653 1.95e-56 201
MS.gene003101.t1 MTR_4g064120 29.211 380 255 5 221 591 249 623 3.65e-56 200
MS.gene003101.t1 MTR_2g097410 29.894 378 257 5 221 593 366 740 6.75e-56 201
MS.gene003101.t1 MTR_4g064180 28.947 380 256 5 221 591 249 623 1.26e-54 196
MS.gene003101.t1 MTR_3g065980 31.218 394 229 11 223 595 169 541 5.23e-54 192
MS.gene003101.t1 MTR_4g104020 32.391 389 238 8 221 595 144 521 3.23e-53 190
MS.gene003101.t1 MTR_7g074650 32.425 367 232 7 229 594 441 792 2.02e-51 189
MS.gene003101.t1 MTR_4g097080 29.379 354 237 5 249 594 155 503 9.30e-49 177
MS.gene003101.t1 MTR_8g442410 30.026 383 252 6 221 594 150 525 1.93e-48 177
MS.gene003101.t1 MTR_3g022830 27.231 437 236 10 217 594 23 436 7.52e-48 173
MS.gene003101.t1 MTR_2g097380 28.818 347 215 4 248 593 245 560 5.65e-45 168
MS.gene003101.t1 MTR_4g102790 31.461 356 223 9 250 593 315 661 9.15e-45 169
MS.gene003101.t1 MTR_5g015490 29.282 362 233 8 249 594 136 490 7.55e-44 163
MS.gene003101.t1 MTR_4g077760 31.445 353 221 10 250 594 216 555 5.44e-41 157
MS.gene003101.t1 MTR_1g096030 30.688 378 208 14 250 594 103 459 4.51e-40 152
MS.gene003101.t1 MTR_4g076020 29.363 361 218 11 243 594 97 429 3.76e-37 144
MS.gene003101.t1 MTR_2g034250 26.357 387 266 7 221 594 205 585 3.95e-37 146
MS.gene003101.t1 MTR_3g072710 27.778 360 225 10 258 595 155 501 5.25e-36 142
MS.gene003101.t1 MTR_2g034260 25.974 385 265 9 223 594 207 584 3.97e-35 140
MS.gene003101.t1 MTR_2g034280 25.714 385 268 9 221 594 198 575 4.42e-35 140
MS.gene003101.t1 MTR_1g069725 28.994 338 218 9 262 595 145 464 3.00e-34 136
MS.gene003101.t1 MTR_7g109580 27.089 347 237 8 255 594 217 554 6.26e-33 133
MS.gene003101.t1 MTR_5g058860 27.654 358 233 12 255 595 150 498 8.89e-33 132
MS.gene003101.t1 MTR_2g099110 27.062 388 252 9 227 594 75 451 2.48e-32 130
MS.gene003101.t1 MTR_2g089100 26.055 403 257 11 223 593 49 442 4.38e-32 129
MS.gene003101.t1 MTR_1g086970 25.429 350 238 8 258 594 139 478 1.92e-28 119
MS.gene003101.t1 MTR_4g026485 25.858 379 252 11 225 595 268 625 1.93e-28 120
MS.gene003101.t1 MTR_5g015950 26.168 321 201 6 307 593 127 445 6.83e-28 117
MS.gene003101.t1 MTR_3g053270 26.168 321 201 6 307 593 127 445 6.83e-28 117
MS.gene003101.t1 MTR_4g095500 24.940 417 262 9 223 593 56 467 1.05e-27 117
MS.gene003101.t1 MTR_5g009080 31.050 219 138 5 227 440 57 267 1.49e-25 110
MS.gene003101.t1 MTR_5g009080 35.915 142 90 1 455 595 338 479 6.98e-23 102
MS.gene003101.t1 MTR_8g093070 27.606 355 215 16 254 593 168 495 1.88e-25 110
MS.gene003101.t1 MTR_4g076140 39.007 141 85 1 455 594 329 469 7.40e-24 105
MS.gene003101.t1 MTR_4g076140 29.952 207 137 3 227 432 49 248 5.20e-23 102
MS.gene003101.t1 MTR_0092s0100 25.330 379 255 11 225 595 374 732 8.06e-24 106
MS.gene003101.t1 MTR_0092s0100 25.330 379 255 11 225 595 371 729 9.33e-24 106
MS.gene003101.t1 MTR_8g020840 24.938 401 249 15 225 594 169 548 5.91e-23 103
MS.gene003101.t1 MTR_3g022580 30.000 110 77 0 485 594 74 183 7.43e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003101.t1 AT4G17230 47.333 600 235 10 1 594 1 525 6.68e-180 519
MS.gene003101.t1 AT4G17230 47.333 600 235 10 1 594 1 525 6.68e-180 519
MS.gene003101.t1 AT5G48150 55.349 430 187 4 167 594 64 490 1.77e-167 486
MS.gene003101.t1 AT5G48150 55.349 430 187 4 167 594 64 490 1.77e-167 486
MS.gene003101.t1 AT5G48150 55.349 430 187 4 167 594 64 490 1.77e-167 486
MS.gene003101.t1 AT5G48150 55.349 430 187 4 167 594 64 490 1.77e-167 486
MS.gene003101.t1 AT1G50600 52.349 447 202 5 150 594 89 526 2.62e-160 469
MS.gene003101.t1 AT2G04890 57.662 385 155 2 210 594 37 413 8.66e-160 463
MS.gene003101.t1 AT1G50600 52.349 447 202 5 150 594 160 597 1.16e-159 470
MS.gene003101.t1 AT1G50600 54.028 422 189 3 174 594 184 601 1.32e-159 470
MS.gene003101.t1 AT1G21450 45.263 380 206 2 216 594 215 593 4.99e-120 368
MS.gene003101.t1 AT1G07530 31.746 378 247 4 222 593 392 764 4.46e-62 219
MS.gene003101.t1 AT3G03450 33.245 376 237 9 223 594 180 545 2.73e-61 213
MS.gene003101.t1 AT5G59450 30.423 378 258 4 221 594 222 598 1.30e-59 209
MS.gene003101.t1 AT5G52510 33.908 348 205 7 262 594 303 640 1.54e-59 210
MS.gene003101.t1 AT5G17490 30.303 495 303 16 124 594 40 516 3.72e-59 206
MS.gene003101.t1 AT3G46600 30.563 373 256 3 223 594 1 371 3.41e-58 199
MS.gene003101.t1 AT3G46600 30.667 375 257 3 221 594 207 579 4.06e-58 205
MS.gene003101.t1 AT3G46600 30.667 375 257 3 221 594 175 547 4.46e-58 204
MS.gene003101.t1 AT2G37650 30.871 379 250 4 221 594 330 701 6.63e-58 206
MS.gene003101.t1 AT2G37650 30.871 379 250 4 221 594 342 713 7.14e-58 207
MS.gene003101.t1 AT1G14920 31.593 383 229 10 223 594 169 529 1.49e-57 202
MS.gene003101.t1 AT2G29060 30.789 380 250 6 222 593 280 654 2.72e-57 204
MS.gene003101.t1 AT2G29060 30.789 380 250 6 222 593 317 691 4.03e-57 204
MS.gene003101.t1 AT2G01570 31.414 382 231 11 223 594 221 581 7.91e-55 196
MS.gene003101.t1 AT1G50420 28.837 430 238 8 227 595 58 480 1.27e-54 193
MS.gene003101.t1 AT1G66350 31.579 380 229 12 223 595 152 507 1.49e-54 193
MS.gene003101.t1 AT2G29065 30.366 382 256 7 221 593 247 627 1.67e-53 193
MS.gene003101.t1 AT1G07520 29.659 381 259 6 221 593 149 528 7.25e-52 186
MS.gene003101.t1 AT1G07520 29.659 381 259 6 221 593 149 528 7.25e-52 186
MS.gene003101.t1 AT1G07520 29.659 381 259 6 221 593 313 692 3.51e-51 187
MS.gene003101.t1 AT1G07520 29.659 381 259 6 221 593 314 693 3.80e-51 187
MS.gene003101.t1 AT3G54220 31.165 369 235 8 229 593 296 649 1.35e-46 174
MS.gene003101.t1 AT5G66770 33.811 349 213 7 254 594 246 584 1.49e-45 170
MS.gene003101.t1 AT3G50650 32.286 350 219 7 254 594 202 542 2.46e-44 166
MS.gene003101.t1 AT1G63100 28.535 389 249 7 226 593 248 628 5.56e-42 160
MS.gene003101.t1 AT1G63100 28.535 389 249 7 226 593 273 653 5.73e-42 161
MS.gene003101.t1 AT4G37650 29.820 389 246 10 227 594 147 529 1.09e-41 158
MS.gene003101.t1 AT5G41920 28.000 375 245 9 227 594 44 400 2.06e-40 152
MS.gene003101.t1 AT1G55580 26.129 310 197 7 304 594 149 445 1.44e-30 125
MS.gene003101.t1 AT4G08250 25.926 351 247 7 250 593 132 476 3.40e-29 121
MS.gene003101.t1 AT3G49950 23.967 363 251 10 249 593 52 407 2.34e-24 106
MS.gene003101.t1 AT3G60630 24.716 352 230 10 260 595 291 623 1.28e-22 102
MS.gene003101.t1 AT2G45160 25.978 358 215 13 259 595 312 640 2.79e-21 98.6
MS.gene003101.t1 AT4G00150 23.873 377 257 11 223 595 205 555 9.27e-20 93.6
MS.gene003101.t1 AT3G13840 23.464 358 245 10 249 594 170 510 2.36e-19 92.0
MS.gene003101.t1 AT4G36710 23.969 388 259 12 221 594 120 485 7.40e-18 87.0
MS.gene003101.t1 AT5G67411 29.101 189 121 5 352 531 14 198 2.12e-12 67.0

Find 111 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTAAGCAAATTGAAAATA+TGG 0.174043 2.2:-60609458 None:intergenic
AAGATTTCAATAAAGATTAT+AGG 0.216624 2.2:+60610221 MS.gene003101:CDS
TCAAAGAACCAATGTTTCTT+TGG 0.242227 2.2:+60608668 MS.gene003101:CDS
TGAGAAAATTGTGGTGAAAA+AGG 0.281191 2.2:-60608807 None:intergenic
GACATGAACTGTTTGGAAAA+TGG 0.289537 2.2:+60610119 MS.gene003101:CDS
AAAGCTCAATTTCTTTCATT+TGG 0.293718 2.2:-60608911 None:intergenic
CCTCCGTTCGTTCGTGTAAC+AGG 0.298231 2.2:+60609608 MS.gene003101:CDS
ACTGTGATCCTTGTGCAATT+TGG 0.334053 2.2:-60609544 None:intergenic
GATTTCAGAGCTGGGCTATA+AGG 0.338435 2.2:+60609043 MS.gene003101:CDS
CTCATCAACTCATTGCTTGT+TGG 0.342988 2.2:-60609407 None:intergenic
TTGCAAATCAATTCAAATAA+TGG 0.345919 2.2:+60608636 MS.gene003101:CDS
GCTGTCGATGATATCTGAAT+TGG 0.360875 2.2:-60609090 None:intergenic
TTAACACTATTGAACTCAAA+TGG 0.361949 2.2:-60609722 None:intergenic
TAAGCACCTAACCTTTGAAT+TGG 0.365266 2.2:-60609311 None:intergenic
TCTTCTTTCAAGTCTAGCTT+TGG 0.368681 2.2:-60609188 None:intergenic
GTTGAAAGACATGAACTGTT+TGG 0.373187 2.2:+60610112 MS.gene003101:CDS
AATTTGAGACCAATCATATT+CGG 0.388708 2.2:-60609153 None:intergenic
TGTGCACAGTTTGTATTTGA+TGG 0.403494 2.2:+60609221 MS.gene003101:CDS
TTTCTTTGGAAACAAGCAAC+TGG 0.406710 2.2:+60608682 MS.gene003101:CDS
GAAAGAAATTGAGCTTTCAT+TGG 0.409492 2.2:+60608917 MS.gene003101:CDS
TTCAATAGTGTTAAAATGTA+TGG 0.409922 2.2:+60609731 MS.gene003101:CDS
TGAATTAATGATTTCAGAGC+TGG 0.411720 2.2:+60609034 MS.gene003101:CDS
TCTGTCTCAAAGACTGTTGC+TGG 0.413091 2.2:-60608584 None:intergenic
GAATCGGAGATTTCATTGTT+AGG 0.417405 2.2:+60609065 MS.gene003101:CDS
GTATGGATGTGAGGTTCAAC+TGG 0.437985 2.2:+60609748 MS.gene003101:CDS
ATCAAAAGCTATGAATTCAT+TGG 0.443080 2.2:-60608742 None:intergenic
AGCTGCCAGTCCAACTTAAA+CGG 0.448434 2.2:+60609110 MS.gene003101:CDS
GCCTAAGGTCGTGTTATTTG+TGG 0.450027 2.2:+60609901 MS.gene003101:CDS
AGAGCAAGGGTGGAATCATC+GGG 0.455587 2.2:+60609350 MS.gene003101:CDS
GGTTCTCCAATTCAAAGGTT+AGG 0.459338 2.2:+60609305 MS.gene003101:CDS
TGTAAGCAAATTGAAAATAT+GGG 0.460197 2.2:-60609457 None:intergenic
GAAATTGAGCTTTCATTGGT+AGG 0.460607 2.2:+60608921 MS.gene003101:CDS
GGTGGAAGACTTGATATTGT+AGG 0.469286 2.2:+60609662 MS.gene003101:CDS
GGTCGTGTTATTTGTGGAGC+AGG 0.469342 2.2:+60609907 MS.gene003101:CDS
GGTACCGATATAGAAGTATT+CGG 0.469425 2.2:+60608942 MS.gene003101:CDS
TGCTTCACAAATGACAGCAT+TGG 0.474342 2.2:-60609486 None:intergenic
GAATAACGTGTTGGGAAAGA+TGG 0.476145 2.2:+60609271 MS.gene003101:CDS
GCTGCCGAATACTTCTATAT+CGG 0.477399 2.2:-60608946 None:intergenic
TCCATGCTCACGCTCTCGTC+CGG 0.480063 2.2:-60609836 None:intergenic
AAGGTACATCCGAATATGAT+TGG 0.489420 2.2:+60609144 MS.gene003101:CDS
GTGAAGGCTACCATTTGAGT+TGG 0.490964 2.2:-60608976 None:intergenic
TTATGTGCACATCTTCTGCT+TGG 0.493993 2.2:+60610290 MS.gene003101:CDS
CTGTCGATGATATCTGAATT+GGG 0.497389 2.2:-60609089 None:intergenic
AAGAGCAAGGGTGGAATCAT+CGG 0.498333 2.2:+60609349 MS.gene003101:CDS
AGCTGTCTGTCCAACTCAAA+TGG 0.502650 2.2:+60608966 MS.gene003101:CDS
GGAAAGATGGTATCGGTTGG+CGG 0.502999 2.2:+60609284 MS.gene003101:CDS
GGATTCATGAATAACGTGTT+GGG 0.509988 2.2:+60609263 MS.gene003101:CDS
AGGATTCATGAATAACGTGT+TGG 0.512384 2.2:+60609262 MS.gene003101:CDS
GGCTACCGTTTAAGTTGGAC+TGG 0.514240 2.2:-60609115 None:intergenic
AGAGCTGGGCTATAAGGAAT+CGG 0.517165 2.2:+60609049 MS.gene003101:CDS
GTGGAGGCTACCGTTTAAGT+TGG 0.523399 2.2:-60609120 None:intergenic
ATTCTCTTCTTATCATCTCT+AGG 0.523465 2.2:-60610022 None:intergenic
GATTCTCCTTCATCTTCTAG+TGG 0.524436 2.2:+60608762 MS.gene003101:CDS
CATTATCTTGAGATGTACCT+TGG 0.526204 2.2:-60608998 None:intergenic
CAAAAGAAACGTAGTTCAGC+CGG 0.533280 2.2:+60608546 MS.gene003101:CDS
GCTTGTTTCCAAAGAAACAT+TGG 0.537666 2.2:-60608676 None:intergenic
GTTAGGTGCTTACATGTTGG+AGG 0.539755 2.2:+60609322 MS.gene003101:CDS
CTTAAAGTCTCAGCAAACCT+TGG 0.540575 2.2:-60609959 None:intergenic
GGTATGTGATGCAAAGCAAA+AGG 0.541821 2.2:-60609815 None:intergenic
GACTGTTGCTGGTAGTATAA+CGG 0.543849 2.2:-60608573 None:intergenic
CATGCTCTCAAGCATAAACC+TGG 0.546708 2.2:+60609581 MS.gene003101:CDS
CGAACGAACGGAGGTCCGCC+AGG 0.546839 2.2:-60609599 None:intergenic
AAGGTTAGGTGCTTACATGT+TGG 0.546985 2.2:+60609319 MS.gene003101:CDS
TTGGCGGTTCTCCAATTCAA+AGG 0.547368 2.2:+60609300 MS.gene003101:CDS
TTCTTTGGAAACAAGCAACT+GGG 0.548975 2.2:+60608683 MS.gene003101:CDS
CTGGTAGTATAACGGTATAC+CGG 0.553841 2.2:-60608565 None:intergenic
ACCGGACGAGAGCGTGAGCA+TGG 0.554808 2.2:+60609835 MS.gene003101:CDS
CTTGAGATGTACCTTGGTGA+AGG 0.559050 2.2:-60608992 None:intergenic
AACACAAATGTATCGCCGTT+AGG 0.563179 2.2:+60608846 MS.gene003101:CDS
TCTTCACCAGTCTCAAAAGC+CGG 0.563592 2.2:-60609871 None:intergenic
ACCGTTACCGTTGAGTACCT+CGG 0.565129 2.2:+60610171 MS.gene003101:CDS
GGAGGGATTAAGAGCAAGGG+TGG 0.565223 2.2:+60609340 MS.gene003101:CDS
GAGTCATAGTGAGAAAATTG+TGG 0.570992 2.2:-60608816 None:intergenic
CATATTCGGATGTACCTTGG+TGG 0.571085 2.2:-60609139 None:intergenic
CAATCGTTTCATGCTAGAGG+TGG 0.577074 2.2:+60609644 MS.gene003101:CDS
ACCGAGGTACTCAACGGTAA+CGG 0.577215 2.2:-60610172 None:intergenic
AATCATATTCGGATGTACCT+TGG 0.577241 2.2:-60609142 None:intergenic
TGTTGGAGGGATTAAGAGCA+AGG 0.578699 2.2:+60609336 MS.gene003101:CDS
TCACAATCGTTTCATGCTAG+AGG 0.578887 2.2:+60609641 MS.gene003101:CDS
TTAGGTGCTTACATGTTGGA+GGG 0.580819 2.2:+60609323 MS.gene003101:CDS
TCCACAAATAACACGACCTT+AGG 0.582649 2.2:-60609902 None:intergenic
GCATGGAGAATCATAGAGAC+CGG 0.589076 2.2:+60609852 MS.gene003101:CDS
GCATTACCACTAGAAGATGA+AGG 0.589681 2.2:-60608768 None:intergenic
GTGGTAATGCATCCAACACC+AGG 0.589942 2.2:+60608781 MS.gene003101:CDS
CGATACATTTGTGTTGTCAG+AGG 0.591652 2.2:-60608838 None:intergenic
CGTTAACATCATAGCTTGTG+AGG 0.593625 2.2:+60610078 MS.gene003101:CDS
ATTCGGATGTACCTTGGTGG+AGG 0.599110 2.2:-60609136 None:intergenic
CGTGTTGGGAAAGATGGTAT+CGG 0.599692 2.2:+60609277 MS.gene003101:CDS
TTGGGAAAGATGGTATCGGT+TGG 0.601702 2.2:+60609281 MS.gene003101:CDS
GGCTACTTGAGAAGATTCCA+AGG 0.613501 2.2:-60608721 None:intergenic
GAATTAATGATTTCAGAGCT+GGG 0.614508 2.2:+60609035 MS.gene003101:CDS
GTTAACATCATAGCTTGTGA+GGG 0.618537 2.2:+60610079 MS.gene003101:CDS
ATGAACTGTTTGGAAAATGG+AGG 0.640291 2.2:+60610122 MS.gene003101:CDS
GCACTAGAGTAAACACCTAA+CGG 0.650576 2.2:-60608861 None:intergenic
ATTGACTTCCAAATTGCACA+AGG 0.655189 2.2:+60609536 MS.gene003101:CDS
GTTAGGTGTTTACTCTAGTG+CGG 0.655711 2.2:+60608863 MS.gene003101:CDS
TGTTAAAATGTATGGATGTG+AGG 0.656156 2.2:+60609739 MS.gene003101:CDS
GTTGGAGGGATTAAGAGCAA+GGG 0.658974 2.2:+60609337 MS.gene003101:CDS
ATGTTGCTATCAATTTAGCG+TGG 0.662028 2.2:+60610257 MS.gene003101:CDS
AATGTTTGAATCAATTGATG+TGG 0.662206 2.2:+60609994 MS.gene003101:CDS
TCAATCACCGAGGTACTCAA+CGG 0.669899 2.2:-60610178 None:intergenic
ATACCTGTTACACGAACGAA+CGG 0.677244 2.2:-60609611 None:intergenic
CCTGTTACACGAACGAACGG+AGG 0.680914 2.2:-60609608 None:intergenic
GTTTACTCTAGTGCGGACGA+TGG 0.696726 2.2:+60608870 MS.gene003101:CDS
TAATGCAGAACAACACTGTG+TGG 0.712966 2.2:+60610045 MS.gene003101:CDS
GCTCTCAAGCATAAACCTGG+CGG 0.733186 2.2:+60609584 MS.gene003101:CDS
TCAAATGGTAGCCTTCACCA+AGG 0.752690 2.2:+60608981 MS.gene003101:CDS
GAGCAAGGGTGGAATCATCG+GGG 0.766989 2.2:+60609351 MS.gene003101:CDS
TTAAACGGTAGCCTCCACCA+AGG 0.778392 2.2:+60609125 MS.gene003101:CDS
TCTAACTGAATCAATCACCG+AGG 0.797585 2.2:-60610188 None:intergenic
AAATTGCACAAGGATCACAG+TGG 0.820097 2.2:+60609546 MS.gene003101:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGATTTCAATAAAGATTAT+AGG + chr2.2:60610221-60610240 MS.gene003101:CDS 15.0%
!! ATGTAAGCAAATTGAAAATA+TGG - chr2.2:60609461-60609480 None:intergenic 20.0%
!! TGTAAGCAAATTGAAAATAT+GGG - chr2.2:60609460-60609479 None:intergenic 20.0%
!! TTCAATAGTGTTAAAATGTA+TGG + chr2.2:60609731-60609750 MS.gene003101:CDS 20.0%
!! TTGCAAATCAATTCAAATAA+TGG + chr2.2:60608636-60608655 MS.gene003101:CDS 20.0%
! AAAGCTCAATTTCTTTCATT+TGG - chr2.2:60608914-60608933 None:intergenic 25.0%
! AATGTTTGAATCAATTGATG+TGG + chr2.2:60609994-60610013 MS.gene003101:CDS 25.0%
! ATCAAAAGCTATGAATTCAT+TGG - chr2.2:60608745-60608764 None:intergenic 25.0%
! TTAACACTATTGAACTCAAA+TGG - chr2.2:60609725-60609744 None:intergenic 25.0%
!! AATTTGAGACCAATCATATT+CGG - chr2.2:60609156-60609175 None:intergenic 25.0%
ATTCTCTTCTTATCATCTCT+AGG - chr2.2:60610025-60610044 None:intergenic 30.0%
GAAAGAAATTGAGCTTTCAT+TGG + chr2.2:60608917-60608936 MS.gene003101:CDS 30.0%
GAATTAATGATTTCAGAGCT+GGG + chr2.2:60609035-60609054 MS.gene003101:CDS 30.0%
TCAAAGAACCAATGTTTCTT+TGG + chr2.2:60608668-60608687 MS.gene003101:CDS 30.0%
TGAATTAATGATTTCAGAGC+TGG + chr2.2:60609034-60609053 MS.gene003101:CDS 30.0%
TGAGAAAATTGTGGTGAAAA+AGG - chr2.2:60608810-60608829 None:intergenic 30.0%
TGTTAAAATGTATGGATGTG+AGG + chr2.2:60609739-60609758 MS.gene003101:CDS 30.0%
! ATGGAGGAAAAGATTTTCAA+TGG + chr2.2:60610138-60610157 MS.gene003101:CDS 30.0%
! GGTGATTTTCAAAAAGCAAT+AGG + chr2.2:60609242-60609261 MS.gene003101:CDS 30.0%
AAGGTACATCCGAATATGAT+TGG + chr2.2:60609144-60609163 MS.gene003101:CDS 35.0%
AATCATATTCGGATGTACCT+TGG - chr2.2:60609145-60609164 None:intergenic 35.0%
AGGATTCATGAATAACGTGT+TGG + chr2.2:60609262-60609281 MS.gene003101:CDS 35.0%
ATGAACTGTTTGGAAAATGG+AGG + chr2.2:60610122-60610141 MS.gene003101:CDS 35.0%
ATGTTGCTATCAATTTAGCG+TGG + chr2.2:60610257-60610276 MS.gene003101:CDS 35.0%
CATTATCTTGAGATGTACCT+TGG - chr2.2:60609001-60609020 None:intergenic 35.0%
CTGTCGATGATATCTGAATT+GGG - chr2.2:60609092-60609111 None:intergenic 35.0%
GAAATTGAGCTTTCATTGGT+AGG + chr2.2:60608921-60608940 MS.gene003101:CDS 35.0%
GAATCGGAGATTTCATTGTT+AGG + chr2.2:60609065-60609084 MS.gene003101:CDS 35.0%
GACATGAACTGTTTGGAAAA+TGG + chr2.2:60610119-60610138 MS.gene003101:CDS 35.0%
GAGTCATAGTGAGAAAATTG+TGG - chr2.2:60608819-60608838 None:intergenic 35.0%
GCTTGTTTCCAAAGAAACAT+TGG - chr2.2:60608679-60608698 None:intergenic 35.0%
GGATTCATGAATAACGTGTT+GGG + chr2.2:60609263-60609282 MS.gene003101:CDS 35.0%
GTTGAAAGACATGAACTGTT+TGG + chr2.2:60610112-60610131 MS.gene003101:CDS 35.0%
TAAGCACCTAACCTTTGAAT+TGG - chr2.2:60609314-60609333 None:intergenic 35.0%
TCAGCAAACCTTGGTAAAAA+TGG - chr2.2:60609953-60609972 None:intergenic 35.0%
TGTGCACAGTTTGTATTTGA+TGG + chr2.2:60609221-60609240 MS.gene003101:CDS 35.0%
! AGGAAAAGATTTTCAATGGC+GGG + chr2.2:60610142-60610161 MS.gene003101:CDS 35.0%
! ATTGACTTCCAAATTGCACA+AGG + chr2.2:60609536-60609555 MS.gene003101:CDS 35.0%
! GAGGAAAAGATTTTCAATGG+CGG + chr2.2:60610141-60610160 MS.gene003101:CDS 35.0%
! GTTAACATCATAGCTTGTGA+GGG + chr2.2:60610079-60610098 MS.gene003101:CDS 35.0%
! TCTTCTTTCAAGTCTAGCTT+TGG - chr2.2:60609191-60609210 None:intergenic 35.0%
! TTCTTTGGAAACAAGCAACT+GGG + chr2.2:60608683-60608702 MS.gene003101:CDS 35.0%
! TTTCTTTGGAAACAAGCAAC+TGG + chr2.2:60608682-60608701 MS.gene003101:CDS 35.0%
! TTTTGCTTTGCATCACATAC+CGG + chr2.2:60609817-60609836 MS.gene003101:CDS 35.0%
!! ACACATCTCCATTTTTACCA+AGG + chr2.2:60609942-60609961 MS.gene003101:CDS 35.0%
!! GGTACCGATATAGAAGTATT+CGG + chr2.2:60608942-60608961 MS.gene003101:CDS 35.0%
AAATTGCACAAGGATCACAG+TGG + chr2.2:60609546-60609565 MS.gene003101:CDS 40.0%
AACACAAATGTATCGCCGTT+AGG + chr2.2:60608846-60608865 MS.gene003101:CDS 40.0%
AAGGTTAGGTGCTTACATGT+TGG + chr2.2:60609319-60609338 MS.gene003101:CDS 40.0%
ACTGTGATCCTTGTGCAATT+TGG - chr2.2:60609547-60609566 None:intergenic 40.0%
ATACCTGTTACACGAACGAA+CGG - chr2.2:60609614-60609633 None:intergenic 40.0%
CAAAAGAAACGTAGTTCAGC+CGG + chr2.2:60608546-60608565 MS.gene003101:CDS 40.0%
CGATACATTTGTGTTGTCAG+AGG - chr2.2:60608841-60608860 None:intergenic 40.0%
CTCATCAACTCATTGCTTGT+TGG - chr2.2:60609410-60609429 None:intergenic 40.0%
CTGGTAGTATAACGGTATAC+CGG - chr2.2:60608568-60608587 None:intergenic 40.0%
CTTAAAGTCTCAGCAAACCT+TGG - chr2.2:60609962-60609981 None:intergenic 40.0%
GAATAACGTGTTGGGAAAGA+TGG + chr2.2:60609271-60609290 MS.gene003101:CDS 40.0%
GACTGTTGCTGGTAGTATAA+CGG - chr2.2:60608576-60608595 None:intergenic 40.0%
GATTCTCCTTCATCTTCTAG+TGG + chr2.2:60608762-60608781 MS.gene003101:CDS 40.0%
GCACTAGAGTAAACACCTAA+CGG - chr2.2:60608864-60608883 None:intergenic 40.0%
GCATTACCACTAGAAGATGA+AGG - chr2.2:60608771-60608790 None:intergenic 40.0%
GCTGCCGAATACTTCTATAT+CGG - chr2.2:60608949-60608968 None:intergenic 40.0%
GCTGTCGATGATATCTGAAT+TGG - chr2.2:60609093-60609112 None:intergenic 40.0%
GGTATGTGATGCAAAGCAAA+AGG - chr2.2:60609818-60609837 None:intergenic 40.0%
GGTGGAAGACTTGATATTGT+AGG + chr2.2:60609662-60609681 MS.gene003101:CDS 40.0%
GGTTCTCCAATTCAAAGGTT+AGG + chr2.2:60609305-60609324 MS.gene003101:CDS 40.0%
GTTAGGTGTTTACTCTAGTG+CGG + chr2.2:60608863-60608882 MS.gene003101:CDS 40.0%
TAATGCAGAACAACACTGTG+TGG + chr2.2:60610045-60610064 MS.gene003101:CDS 40.0%
TCACAATCGTTTCATGCTAG+AGG + chr2.2:60609641-60609660 MS.gene003101:CDS 40.0%
TCCACAAATAACACGACCTT+AGG - chr2.2:60609905-60609924 None:intergenic 40.0%
TCTAACTGAATCAATCACCG+AGG - chr2.2:60610191-60610210 None:intergenic 40.0%
TGAAAAAGGATTCCTGGTGT+TGG - chr2.2:60608796-60608815 None:intergenic 40.0%
TGCTTCACAAATGACAGCAT+TGG - chr2.2:60609489-60609508 None:intergenic 40.0%
TTAGGTGCTTACATGTTGGA+GGG + chr2.2:60609323-60609342 MS.gene003101:CDS 40.0%
TTATGTGCACATCTTCTGCT+TGG + chr2.2:60610290-60610309 MS.gene003101:CDS 40.0%
TTGTGGTGAAAAAGGATTCC+TGG - chr2.2:60608802-60608821 None:intergenic 40.0%
! CGTTAACATCATAGCTTGTG+AGG + chr2.2:60610078-60610097 MS.gene003101:CDS 40.0%
! GGAGAAGCAATTTTGTACCT+TGG + chr2.2:60608704-60608723 MS.gene003101:CDS 40.0%
AAGAGCAAGGGTGGAATCAT+CGG + chr2.2:60609349-60609368 MS.gene003101:CDS 45.0%
AGAGCTGGGCTATAAGGAAT+CGG + chr2.2:60609049-60609068 MS.gene003101:CDS 45.0%
AGCTGCCAGTCCAACTTAAA+CGG + chr2.2:60609110-60609129 MS.gene003101:CDS 45.0%
AGCTGTCTGTCCAACTCAAA+TGG + chr2.2:60608966-60608985 MS.gene003101:CDS 45.0%
CAATCGTTTCATGCTAGAGG+TGG + chr2.2:60609644-60609663 MS.gene003101:CDS 45.0%
CATATTCGGATGTACCTTGG+TGG - chr2.2:60609142-60609161 None:intergenic 45.0%
CATGCTCTCAAGCATAAACC+TGG + chr2.2:60609581-60609600 MS.gene003101:CDS 45.0%
CGTGTTGGGAAAGATGGTAT+CGG + chr2.2:60609277-60609296 MS.gene003101:CDS 45.0%
CTTGAGATGTACCTTGGTGA+AGG - chr2.2:60608995-60609014 None:intergenic 45.0%
GATTTCAGAGCTGGGCTATA+AGG + chr2.2:60609043-60609062 MS.gene003101:CDS 45.0%
GCATGGAGAATCATAGAGAC+CGG + chr2.2:60609852-60609871 MS.gene003101:CDS 45.0%
GGCTACTTGAGAAGATTCCA+AGG - chr2.2:60608724-60608743 None:intergenic 45.0%
GTATGGATGTGAGGTTCAAC+TGG + chr2.2:60609748-60609767 MS.gene003101:CDS 45.0%
GTGAAGGCTACCATTTGAGT+TGG - chr2.2:60608979-60608998 None:intergenic 45.0%
GTTAGGTGCTTACATGTTGG+AGG + chr2.2:60609322-60609341 MS.gene003101:CDS 45.0%
GTTGGAGGGATTAAGAGCAA+GGG + chr2.2:60609337-60609356 MS.gene003101:CDS 45.0%
TCAAATGGTAGCCTTCACCA+AGG + chr2.2:60608981-60609000 MS.gene003101:CDS 45.0%
TCTGTCTCAAAGACTGTTGC+TGG - chr2.2:60608587-60608606 None:intergenic 45.0%
TCTTCACCAGTCTCAAAAGC+CGG - chr2.2:60609874-60609893 None:intergenic 45.0%
TGTTGGAGGGATTAAGAGCA+AGG + chr2.2:60609336-60609355 MS.gene003101:CDS 45.0%
TTGGCGGTTCTCCAATTCAA+AGG + chr2.2:60609300-60609319 MS.gene003101:CDS 45.0%
! GGTGAAGATTTTGTCGCCTA+AGG + chr2.2:60609886-60609905 MS.gene003101:CDS 45.0%
! TCAATCACCGAGGTACTCAA+CGG - chr2.2:60610181-60610200 None:intergenic 45.0%
!! GCCTAAGGTCGTGTTATTTG+TGG + chr2.2:60609901-60609920 MS.gene003101:CDS 45.0%
!! TTGGGAAAGATGGTATCGGT+TGG + chr2.2:60609281-60609300 MS.gene003101:CDS 45.0%
ACCGTTACCGTTGAGTACCT+CGG + chr2.2:60610171-60610190 MS.gene003101:CDS 50.0%
AGAGCAAGGGTGGAATCATC+GGG + chr2.2:60609350-60609369 MS.gene003101:CDS 50.0%
ATTCGGATGTACCTTGGTGG+AGG - chr2.2:60609139-60609158 None:intergenic 50.0%
GCTCTCAAGCATAAACCTGG+CGG + chr2.2:60609584-60609603 MS.gene003101:CDS 50.0%
GTGGAGGCTACCGTTTAAGT+TGG - chr2.2:60609123-60609142 None:intergenic 50.0%
GTTTACTCTAGTGCGGACGA+TGG + chr2.2:60608870-60608889 MS.gene003101:CDS 50.0%
TTAAACGGTAGCCTCCACCA+AGG + chr2.2:60609125-60609144 MS.gene003101:CDS 50.0%
! ACCGAGGTACTCAACGGTAA+CGG - chr2.2:60610175-60610194 None:intergenic 50.0%
! GTGGTAATGCATCCAACACC+AGG + chr2.2:60608781-60608800 MS.gene003101:CDS 50.0%
!! GGAAAGATGGTATCGGTTGG+CGG + chr2.2:60609284-60609303 MS.gene003101:CDS 50.0%
!! GGCTACCGTTTAAGTTGGAC+TGG - chr2.2:60609118-60609137 None:intergenic 50.0%
!! GGTCGTGTTATTTGTGGAGC+AGG + chr2.2:60609907-60609926 MS.gene003101:CDS 50.0%
!!! TAGAGACCGGCTTTTGAGAC+TGG + chr2.2:60609865-60609884 MS.gene003101:CDS 50.0%
CCTCCGTTCGTTCGTGTAAC+AGG + chr2.2:60609608-60609627 MS.gene003101:CDS 55.0%
CCTGTTACACGAACGAACGG+AGG - chr2.2:60609611-60609630 None:intergenic 55.0%
GAGCAAGGGTGGAATCATCG+GGG + chr2.2:60609351-60609370 MS.gene003101:CDS 55.0%
GGAGGGATTAAGAGCAAGGG+TGG + chr2.2:60609340-60609359 MS.gene003101:CDS 55.0%
TCCATGCTCACGCTCTCGTC+CGG - chr2.2:60609839-60609858 None:intergenic 60.0%
! ACCGGACGAGAGCGTGAGCA+TGG + chr2.2:60609835-60609854 MS.gene003101:CDS 65.0%
CGAACGAACGGAGGTCCGCC+AGG - chr2.2:60609602-60609621 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 60608534 60610324 60608534 ID=MS.gene003101
chr2.2 mRNA 60608534 60610324 60608534 ID=MS.gene003101.t1;Parent=MS.gene003101
chr2.2 exon 60608534 60610324 60608534 ID=MS.gene003101.t1.exon1;Parent=MS.gene003101.t1
chr2.2 CDS 60608534 60610324 60608534 ID=cds.MS.gene003101.t1;Parent=MS.gene003101.t1
Gene Sequence

>MS.gene003101

ATGTATACTTCTCAAAAGAAACGTAGTTCAGCCGGTATACCGTTATACTACCAGCAACAGTCTTTGAGACAGATTGATTCAAACAACAATCGTTTCAAAACGTTGCAAATCAATTCAAATAATGGTAGTATCAGTCAAAGAACCAATGTTTCTTTGGAAACAAGCAACTGGGAGAAGCAATTTTGTACCTTGGAATCTTCTCAAGTAGCCAATGAATTCATAGCTTTTGATTCTCCTTCATCTTCTAGTGGTAATGCATCCAACACCAGGAATCCTTTTTCACCACAATTTTCTCACTATGACTCCTCTGACAACACAAATGTATCGCCGTTAGGTGTTTACTCTAGTGCGGACGATGGTAGCTTTGAATTGAAGCACCAAATGAAAGAAATTGAGCTTTCATTGGTAGGTACCGATATAGAAGTATTCGGCAGCTGTCTGTCCAACTCAAATGGTAGCCTTCACCAAGGTACATCTCAAGATAATGAGTTTCAAATTGATGAATTAATGATTTCAGAGCTGGGCTATAAGGAATCGGAGATTTCATTGTTAGGTCCCAATTCAGATATCATCGACAGCTGCCAGTCCAACTTAAACGGTAGCCTCCACCAAGGTACATCCGAATATGATTGGTCTCAAATTGTAGAAATGATTCCAAAGCTAGACTTGAAAGAAGAACTTATTAGATGTGCACAGTTTGTATTTGATGGTGATTTTCAAAAAGCAATAGGATTCATGAATAACGTGTTGGGAAAGATGGTATCGGTTGGCGGTTCTCCAATTCAAAGGTTAGGTGCTTACATGTTGGAGGGATTAAGAGCAAGGGTGGAATCATCGGGGAGTGCAATTTATAATGCGTTGAAATGTGAAGAACCAACAAGCAATGAGTTGATGAGTGCTATGCACACTTTGTATCAGATTTGCCCATATTTTCAATTTGCTTACATATCATCCAATGCTGTCATTTGTGAAGCAATGCAAAATGAATCAAGAATTCATATAATTGACTTCCAAATTGCACAAGGATCACAGTGGATGTTACTTTTGCATGCTCTCAAGCATAAACCTGGCGGACCTCCGTTCGTTCGTGTAACAGGTATCGATGATTCACAATCGTTTCATGCTAGAGGTGGAAGACTTGATATTGTAGGAAAAAAGCTAGAAGATTGTGCAAAAACTTGTAGAGTTCCATTTGAGTTCAATAGTGTTAAAATGTATGGATGTGAGGTTCAACTGGAAGATTTTGAAGTTCAACATGATGAAGTTCTTGCTGTGAATTTTCCTTTTGCTTTGCATCACATACCGGACGAGAGCGTGAGCATGGAGAATCATAGAGACCGGCTTTTGAGACTGGTGAAGATTTTGTCGCCTAAGGTCGTGTTATTTGTGGAGCAGGAGTCAAACACAAACACATCTCCATTTTTACCAAGGTTTGCTGAGACTTTAAGTTATTACACTGCAATGTTTGAATCAATTGATGTGGCTCTTCCTAGAGATGATAAGAAGAGAATTAATGCAGAACAACACTGTGTGGCTCGCGATATCGTTAACATCATAGCTTGTGAGGGAGATGAGAGAGTTGAAAGACATGAACTGTTTGGAAAATGGAGGAAAAGATTTTCAATGGCGGGTTTTGTACCGTTACCGTTGAGTACCTCGGTGATTGATTCAGTTAGAACTCTCTTGAAAGATTTCAATAAAGATTATAGGATTGAAGAAACAGATGTTGCTATCAATTTAGCGTGGAAGAGTAAAGTTATGTGCACATCTTCTGCTTGGAGATGTTATTGA
Protein sequence

>MS.gene003101.t1

MYTSQKKRSSAGIPLYYQQQSLRQIDSNNNRFKTLQINSNNGSISQRTNVSLETSNWEKQFCTLESSQVANEFIAFDSPSSSSGNASNTRNPFSPQFSHYDSSDNTNVSPLGVYSSADDGSFELKHQMKEIELSLVGTDIEVFGSCLSNSNGSLHQGTSQDNEFQIDELMISELGYKESEISLLGPNSDIIDSCQSNLNGSLHQGTSEYDWSQIVEMIPKLDLKEELIRCAQFVFDGDFQKAIGFMNNVLGKMVSVGGSPIQRLGAYMLEGLRARVESSGSAIYNALKCEEPTSNELMSAMHTLYQICPYFQFAYISSNAVICEAMQNESRIHIIDFQIAQGSQWMLLLHALKHKPGGPPFVRVTGIDDSQSFHARGGRLDIVGKKLEDCAKTCRVPFEFNSVKMYGCEVQLEDFEVQHDEVLAVNFPFALHHIPDESVSMENHRDRLLRLVKILSPKVVLFVEQESNTNTSPFLPRFAETLSYYTAMFESIDVALPRDDKKRINAEQHCVARDIVNIIACEGDERVERHELFGKWRKRFSMAGFVPLPLSTSVIDSVRTLLKDFNKDYRIEETDVAINLAWKSKVMCTSSAWRCY