AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003126

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003126.t1 MTR_2g026725 94.987 379 18 1 1 379 1 378 0.0 652
MS.gene003126.t1 MTR_1g098900 39.550 311 146 8 77 351 238 542 1.61e-54 190
MS.gene003126.t1 MTR_1g098900 41.379 87 50 1 263 349 57 142 2.29e-15 78.2
MS.gene003126.t1 MTR_1g098870 37.308 260 121 6 106 351 229 460 2.60e-43 158
MS.gene003126.t1 MTR_1g098870 42.529 87 49 1 263 349 55 140 3.86e-15 77.4
MS.gene003126.t1 MTR_1g081180 31.153 321 190 7 84 373 210 530 5.17e-35 135
MS.gene003126.t1 MTR_6g035370 60.870 92 36 0 260 351 461 552 3.51e-33 130
MS.gene003126.t1 MTR_6g035370 38.710 93 56 1 263 355 55 146 1.03e-15 79.0
MS.gene003126.t1 MTR_7g114860 31.395 258 135 8 115 350 240 477 3.13e-29 118
MS.gene003126.t1 MTR_1g492790 26.400 250 133 8 92 332 277 484 6.73e-16 79.3
MS.gene003126.t1 MTR_1g492790 33.333 132 83 4 219 349 73 200 3.06e-13 71.2
MS.gene003126.t1 MTR_1g060990 37.755 98 59 2 254 350 122 218 8.28e-14 73.2
MS.gene003126.t1 MTR_5g026540 31.683 101 64 2 259 357 41 138 7.23e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003126.t1 AT5G47660 41.365 249 121 3 106 354 169 392 2.58e-51 176
MS.gene003126.t1 AT1G76890 37.838 259 131 5 106 354 252 490 2.22e-44 161
MS.gene003126.t1 AT1G76890 41.176 85 49 1 263 347 41 124 2.88e-14 74.7
MS.gene003126.t1 AT5G28300 35.361 263 138 8 115 350 299 556 2.78e-37 142
MS.gene003126.t1 AT1G76880 53.398 103 44 1 256 358 401 499 5.46e-31 124
MS.gene003126.t1 AT1G76880 41.379 87 50 1 263 349 61 146 2.00e-14 75.1
MS.gene003126.t1 AT1G33240 54.839 93 42 0 263 355 435 527 5.38e-29 118
MS.gene003126.t1 AT1G33240 39.080 87 52 1 263 349 62 147 2.21e-13 72.0
MS.gene003126.t1 AT1G33240 54.839 93 42 0 263 355 435 527 7.06e-29 118
MS.gene003126.t1 AT1G33240 39.080 87 52 1 263 349 62 147 2.14e-13 72.0
MS.gene003126.t1 AT1G33240 54.839 93 42 0 263 355 396 488 1.03e-28 118
MS.gene003126.t1 AT5G03680 28.881 277 163 7 76 343 255 506 2.11e-18 87.4
MS.gene003126.t1 AT5G03680 41.573 89 50 2 263 350 119 206 1.68e-14 75.5
MS.gene003126.t1 AT3G10000 35.433 127 77 3 225 350 54 176 1.89e-16 81.3
MS.gene003126.t1 AT3G10000 33.333 126 71 3 225 350 54 166 1.35e-13 72.4

Find 140 sgRNAs with CRISPR-Local

Find 188 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGACGGGTCCTCGACCTTTA+TGG 0.221031 2.1:+63571481 None:intergenic
ATCGCTGATTCAGAAACTAT+TGG 0.316999 2.1:-63570270 MS.gene003126:CDS
TTGCCCTTACTTTGACGAAT+TGG 0.318055 2.1:-63569796 MS.gene003126:CDS
GGAGATAGCTGGAGCATTGC+AGG 0.341688 2.1:-63569931 MS.gene003126:CDS
TGGGATCGAAAGGATCAATA+TGG 0.344397 2.1:-63569956 MS.gene003126:CDS
TTGGACAATCTGTATAGAAA+TGG 0.356199 2.1:-63569777 MS.gene003126:CDS
GGAACACAAGTTCGGTCATA+TGG 0.359379 2.1:-63569976 MS.gene003126:CDS
CGGCCCCATGTACTGGATTA+AGG 0.361071 2.1:+63570103 None:intergenic
CGAAGAAGTGCAAGGAGAAA+TGG 0.367920 2.1:-63569887 MS.gene003126:CDS
CCTCAGCCGGCCCCATGTAC+TGG 0.380046 2.1:+63570096 None:intergenic
GGGAAACCAGGAGGAGGAGG+AGG 0.384212 2.1:+63571530 None:intergenic
TCGCTGATTCAGAAACTATT+GGG 0.386057 2.1:-63570269 MS.gene003126:CDS
CTTATTCCGCTTCCTTCTGC+TGG 0.387343 2.1:+63570224 None:intergenic
TCTGCTAATACTAAAGAATT+CGG 0.401638 2.1:-63570527 MS.gene003126:CDS
TTCCCTTATGGAAACATTGT+TGG 0.404001 2.1:+63569719 None:intergenic
GGTAGAGGGGTGTGGTGAGA+AGG 0.405204 2.1:+63571509 None:intergenic
GTCGAGGACCCGTCTCGATC+CGG 0.413921 2.1:-63571473 MS.gene003126:CDS
CCCTCTACCACCTTCCATAA+AGG 0.418335 2.1:-63571495 MS.gene003126:CDS
AGGTCGCCGGAGTCGGGAAA+TGG 0.420361 2.1:+63571629 None:intergenic
AGCTGGAGCATTGCAGGGCA+TGG 0.423836 2.1:-63569925 MS.gene003126:CDS
AAAACATCAATAAGTACTAT+AGG 0.424007 2.1:-63569863 MS.gene003126:CDS
GAAGGGAAACCAGGAGGAGG+AGG 0.425874 2.1:+63571527 None:intergenic
TTGTTATTGCTCACCTCAGC+CGG 0.426631 2.1:+63570083 None:intergenic
GCGATCCAAGTAACAGTAGA+TGG 0.427115 2.1:-63570043 MS.gene003126:CDS
TCATCATTTCAGCTCGTCTT+CGG 0.431474 2.1:-63570567 MS.gene003126:intron
CGACCCCACGTTGTGGGTCA+AGG 0.431970 2.1:+63570157 None:intergenic
TTGTCCAATTCGTCAAAGTA+AGG 0.432653 2.1:+63569792 None:intergenic
AATGGGTCCTTGGAGAGAAA+TGG 0.436187 2.1:-63569759 MS.gene003126:CDS
GGGGATTGTGGTGGTTGGAG+AGG 0.440819 2.1:+63571588 None:intergenic
AGAGCAGATGCATAAAGAAT+TGG 0.442580 2.1:-63570423 MS.gene003126:CDS
ATACCAACAATGTTTCCATA+AGG 0.443747 2.1:-63569722 MS.gene003126:CDS
AGCATGGAAGCGCGAGGAGA+TGG 0.446602 2.1:-63570354 MS.gene003126:CDS
CCTAGCCTTAATCCAGTACA+TGG 0.450131 2.1:-63570108 MS.gene003126:CDS
TTGCAGCAAATTTGGTAAAG+AGG 0.450584 2.1:-63570460 MS.gene003126:CDS
ACCTTTATGGAAGGTGGTAG+AGG 0.453928 2.1:+63571494 None:intergenic
AGCGCTTGTACTTCAACATC+TGG 0.456538 2.1:+63570020 None:intergenic
GTTTCTGAATCAGCGATATG+AGG 0.456889 2.1:+63570276 None:intergenic
AAGATAAGGTCGCCGGAGTC+GGG 0.457626 2.1:+63571623 None:intergenic
AGGAGGAGGGATGGACCTGA+TGG 0.458333 2.1:+63571553 None:intergenic
GAGAAAGAGTTCGAGAAAGC+TGG 0.464906 2.1:-63570489 MS.gene003126:CDS
TGTTTCCATAAGGGAAGAAA+AGG 0.473178 2.1:-63569712 MS.gene003126:CDS
GACCCGTCTCGATCCGGGTC+GGG 0.479636 2.1:-63571467 MS.gene003126:CDS
TGGACAATCTGTATAGAAAT+GGG 0.482755 2.1:-63569776 MS.gene003126:CDS
GTGTGGTGAGAAGGGAAACC+AGG 0.486892 2.1:+63571518 None:intergenic
CCGCTTCCTTCTGCTGGTGG+AGG 0.487836 2.1:+63570230 None:intergenic
CCAGTACATGGGGCCGGCTG+AGG 0.488463 2.1:-63570096 MS.gene003126:CDS
GTAGAGGGGTGTGGTGAGAA+GGG 0.489814 2.1:+63571510 None:intergenic
TCGAGGACCCGTCTCGATCC+GGG 0.493085 2.1:-63571472 MS.gene003126:CDS
GACCCGACCCGGATCGAGAC+GGG 0.496300 2.1:+63571465 None:intergenic
TCTGGCCATCTACTGTTACT+TGG 0.497676 2.1:+63570038 None:intergenic
GAAGATAAGGTCGCCGGAGT+CGG 0.499156 2.1:+63571622 None:intergenic
TGGAAGGTGGTAGAGGGGTG+TGG 0.500655 2.1:+63571501 None:intergenic
CCACAACGTGGGGTCGGCTG+AGG 0.501288 2.1:-63570150 MS.gene003126:CDS
CTCAGCCGACCCCACGTTGT+GGG 0.501316 2.1:+63570151 None:intergenic
TCAATATGGGAGGAGATAGC+TGG 0.501950 2.1:-63569942 MS.gene003126:CDS
TGCATTTCCATTTCTCTCCA+AGG 0.502699 2.1:+63569752 None:intergenic
AAGAGAAGAGGATGAAGTTG+AGG 0.502881 2.1:-63570204 MS.gene003126:CDS
TCTGTATAGAAATGGGTCCT+TGG 0.503578 2.1:-63569769 MS.gene003126:CDS
GATTGTGGTGGTTGGAGAGG+AGG 0.505297 2.1:+63571591 None:intergenic
GCAGATGCATAAAGAATTGG+TGG 0.506304 2.1:-63570420 MS.gene003126:CDS
TTGCCCCTTTGTCAAGAACC+CGG 0.506347 2.1:-63571434 MS.gene003126:CDS
TTACCCGGGTTCTTGACAAA+GGG 0.507083 2.1:+63571430 None:intergenic
GGAGGAGGGATGGACCTGAT+GGG 0.508730 2.1:+63571554 None:intergenic
GGAGGCCGGCGGCGAAGATA+AGG 0.508923 2.1:+63571609 None:intergenic
TACCAACAATGTTTCCATAA+GGG 0.510070 2.1:-63569721 MS.gene003126:CDS
ATTCCGCTTCCTTCTGCTGG+TGG 0.513116 2.1:+63570227 None:intergenic
GTGGTGGTTGGAGAGGAGGC+CGG 0.515979 2.1:+63571595 None:intergenic
CAGTTGGGAGCGGCAAGAAA+CGG 0.517486 2.1:-63569836 MS.gene003126:CDS
GGATAAAGAGAGAAGAAGCA+TGG 0.520205 2.1:-63570370 MS.gene003126:CDS
ACCGCATTGGAACACAAGTT+CGG 0.524498 2.1:-63569984 MS.gene003126:CDS
GAAGAAGTGCAAGGAGAAAT+GGG 0.526951 2.1:-63569886 MS.gene003126:CDS
AGTACTATAGGAGGACAGTT+GGG 0.527630 2.1:-63569851 MS.gene003126:CDS
AATGATTGAGAGGATGGAAA+AGG 0.532492 2.1:-63570396 MS.gene003126:CDS
CCCACGTTGTGGGTCAAGGG+TGG 0.532882 2.1:+63570161 None:intergenic
CATAAGGGAAGAAAAGGAGC+AGG 0.532990 2.1:-63569706 MS.gene003126:CDS
CCTCAGCCGACCCCACGTTG+TGG 0.533841 2.1:+63570150 None:intergenic
GGTGGAAATGATTGAGAGGA+TGG 0.536865 2.1:-63570402 MS.gene003126:CDS
GCTGGAGCATTGCAGGGCAT+GGG 0.538046 2.1:-63569924 MS.gene003126:CDS
CAGCTCGTCTTCGGATGACG+TGG 0.539019 2.1:-63570558 MS.gene003126:CDS
CCTTTATGGAAGGTGGTAGA+GGG 0.540722 2.1:+63571495 None:intergenic
GAGGAGGGATGGACCTGATG+GGG 0.543104 2.1:+63571555 None:intergenic
GAACACAAGTTCGGTCATAT+GGG 0.545310 2.1:-63569975 MS.gene003126:CDS
CCATGTACTGGATTAAGGCT+AGG 0.546605 2.1:+63570108 None:intergenic
GGAGATGGAGAGAATTAAGC+AGG 0.547111 2.1:-63570339 MS.gene003126:CDS
GGGATCGAAAGGATCAATAT+GGG 0.547722 2.1:-63569955 MS.gene003126:CDS
GTTGCTCCATTTCCCGACTC+CGG 0.549274 2.1:-63571635 MS.gene003126:CDS
CCACGTTGTGGGTCAAGGGT+GGG 0.550717 2.1:+63570162 None:intergenic
GACCCCACGTTGTGGGTCAA+GGG 0.551673 2.1:+63570158 None:intergenic
CTGGATTAAGGCTAGGCTGT+TGG 0.556448 2.1:+63570115 None:intergenic
AAGTACTATAGGAGGACAGT+TGG 0.558326 2.1:-63569852 MS.gene003126:CDS
TTCGGTCATATGGGATCGAA+AGG 0.560164 2.1:-63569966 MS.gene003126:CDS
CATAGCAGTAAGAACCGCAT+TGG 0.560429 2.1:-63569997 MS.gene003126:CDS
CCTCCACCAGCAGAAGGAAG+CGG 0.560638 2.1:-63570230 MS.gene003126:CDS
GGACCCGTCTCGATCCGGGT+CGG 0.561874 2.1:-63571468 MS.gene003126:CDS
TGAGAAGGGAAACCAGGAGG+AGG 0.562570 2.1:+63571524 None:intergenic
CAAAGAAGAATGACCCGACC+CGG 0.563112 2.1:+63571454 None:intergenic
GAGGAGGTTCATGCTGTCCA+TGG 0.563553 2.1:+63571670 None:intergenic
CTTGACCCACAACGTGGGGT+CGG 0.569379 2.1:-63570156 MS.gene003126:CDS
GGCGACCTTATCTTCGCCGC+CGG 0.577427 2.1:-63571614 MS.gene003126:CDS
CTTACCCGGGTTCTTGACAA+AGG 0.581461 2.1:+63571429 None:intergenic
TCCTCGACCTTTATGGAAGG+TGG 0.582785 2.1:+63571488 None:intergenic
TTTCTGAATCAGCGATATGA+GGG 0.586770 2.1:+63570277 None:intergenic
TGCCCCTTTGTCAAGAACCC+GGG 0.588206 2.1:-63571433 MS.gene003126:intron
AGCTCGTCTTCGGATGACGT+GGG 0.593056 2.1:-63570557 MS.gene003126:CDS
CAGATTCCTCCACCAGCAGA+AGG 0.594230 2.1:-63570236 MS.gene003126:CDS
CCCACCCTTGACCCACAACG+TGG 0.596089 2.1:-63570162 MS.gene003126:CDS
CTTAATCCAGTACATGGGGC+CGG 0.598105 2.1:-63570102 MS.gene003126:CDS
CACCCTTGACCCACAACGTG+GGG 0.600798 2.1:-63570160 MS.gene003126:CDS
AATTCGGCTCAAGAAAGAGA+AGG 0.601809 2.1:-63570511 MS.gene003126:CDS
CCACCCTTGACCCACAACGT+GGG 0.601931 2.1:-63570161 MS.gene003126:CDS
GGCGGCGAAGATAAGGTCGC+CGG 0.602029 2.1:+63571616 None:intergenic
TGTCCAATTCGTCAAAGTAA+GGG 0.602664 2.1:+63569793 None:intergenic
GAGCAACATGGTCGGAGGCG+AGG 0.603241 2.1:+63571651 None:intergenic
TGACCCGACCCGGATCGAGA+CGG 0.603647 2.1:+63571464 None:intergenic
TTGAGAGGATGGAAAAGGAA+AGG 0.604583 2.1:-63570391 MS.gene003126:CDS
ACCACCTTCCATAAAGGTCG+AGG 0.606661 2.1:-63571489 MS.gene003126:CDS
TATAGGAGGACAGTTGGGAG+CGG 0.607290 2.1:-63569846 MS.gene003126:CDS
CTAGCCTTAATCCAGTACAT+GGG 0.619110 2.1:-63570107 MS.gene003126:CDS
CAACATGGTCGGAGGCGAGG+AGG 0.621911 2.1:+63571654 None:intergenic
ACATCAATAAGTACTATAGG+AGG 0.623811 2.1:-63569860 MS.gene003126:CDS
GAGATAGCTGGAGCATTGCA+GGG 0.625252 2.1:-63569930 MS.gene003126:CDS
GTGGTTGGAGAGGAGGCCGG+CGG 0.625523 2.1:+63571598 None:intergenic
GCAGGACGAAGAAGCTAGAG+CGG 0.631131 2.1:-63570321 MS.gene003126:CDS
AATTGGTGGAAATGATTGAG+AGG 0.633368 2.1:-63570406 MS.gene003126:CDS
AAATGGAGCAACATGGTCGG+AGG 0.635563 2.1:+63571646 None:intergenic
GGGAAATGGAGCAACATGGT+CGG 0.636053 2.1:+63571643 None:intergenic
AGAAGAAGCATGGAAGCGCG+AGG 0.638350 2.1:-63570360 MS.gene003126:CDS
TCGTTCTGCGAAGAAGTGCA+AGG 0.647147 2.1:-63569895 MS.gene003126:CDS
AGGAAGCGGAATAAGAGAAG+AGG 0.647234 2.1:-63570216 MS.gene003126:CDS
AAGAAGCTAGAGCGGCTGAG+AGG 0.648990 2.1:-63570313 MS.gene003126:CDS
GGGTCCTCGACCTTTATGGA+AGG 0.655244 2.1:+63571485 None:intergenic
CTTTATGGAAGGTGGTAGAG+GGG 0.657356 2.1:+63571496 None:intergenic
AGTCGGGAAATGGAGCAACA+TGG 0.658925 2.1:+63571639 None:intergenic
AAATTTGGTAAAGAGGATGA+CGG 0.659081 2.1:-63570453 MS.gene003126:CDS
ACCGAACTTGTGTTCCAATG+CGG 0.661234 2.1:+63569983 None:intergenic
TACCCGGGTTCTTGACAAAG+GGG 0.666977 2.1:+63571431 None:intergenic
ATCGAAAGGATCAATATGGG+AGG 0.667283 2.1:-63569952 MS.gene003126:CDS
TGGTGAGAAGGGAAACCAGG+AGG 0.688570 2.1:+63571521 None:intergenic
TAGCCTTAATCCAGTACATG+GGG 0.692795 2.1:-63570106 MS.gene003126:CDS
ATAAGGGAAGAAAAGGAGCA+GGG 0.692895 2.1:-63569705 MS.gene003126:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTTTATCTTTTTTATTT+TGG - chr2.1:63570014-63570033 MS.gene003126:CDS 10.0%
!!! ATTGTAAATGTTTTGTTTTA+TGG - chr2.1:63570346-63570365 MS.gene003126:CDS 15.0%
!! AAAACATCAATAAGTACTAT+AGG - chr2.1:63571500-63571519 MS.gene003126:CDS 20.0%
!! AATTCAATGGTTAAACAATT+AGG - chr2.1:63570042-63570061 MS.gene003126:CDS 20.0%
!! AGCATATATAATAGTTCATA+AGG - chr2.1:63570613-63570632 MS.gene003126:intron 20.0%
!! ATATATGCTACATAAAAACT+TGG + chr2.1:63570602-63570621 None:intergenic 20.0%
!!! ACAATTTTTGTCAATTTTCA+TGG - chr2.1:63570271-63570290 MS.gene003126:CDS 20.0%
!!! TATTTTGGTTGAAAATTCAA+TGG - chr2.1:63570029-63570048 MS.gene003126:CDS 20.0%
! AACAAAAAACATTTCAACAG+CGG - chr2.1:63571298-63571317 MS.gene003126:intron 25.0%
! AGTAAAAACTTTACAAGCTA+AGG + chr2.1:63569973-63569992 None:intergenic 25.0%
! ATTCGAATCATTATCGTAAA+AGG + chr2.1:63570768-63570787 None:intergenic 25.0%
! GAAGAAAAAAGAATCATACA+AGG - chr2.1:63570381-63570400 MS.gene003126:CDS 25.0%
! GTAAAAACTTTACAAGCTAA+GGG + chr2.1:63569972-63569991 None:intergenic 25.0%
! TCTGCTAATACTAAAGAATT+CGG - chr2.1:63570836-63570855 MS.gene003126:intron 25.0%
! TTGTAGTATGAATTTGGATT+TGG - chr2.1:63570406-63570425 MS.gene003126:CDS 25.0%
! TTTACGTTAGTCAAACAATT+AGG - chr2.1:63570461-63570480 MS.gene003126:CDS 25.0%
!! ATTTGAATTTTCGATCATTG+TGG - chr2.1:63570429-63570448 MS.gene003126:CDS 25.0%
!!! AAGGTATTGTAGTATGAATT+TGG - chr2.1:63570400-63570419 MS.gene003126:CDS 25.0%
AAATTTGGTAAAGAGGATGA+CGG - chr2.1:63570910-63570929 MS.gene003126:intron 30.0%
ACATCAATAAGTACTATAGG+AGG - chr2.1:63571503-63571522 MS.gene003126:CDS 30.0%
AGTCAAACAATTAGGACTAT+TGG - chr2.1:63570469-63570488 MS.gene003126:CDS 30.0%
ATACCAACAATGTTTCCATA+AGG - chr2.1:63571641-63571660 MS.gene003126:CDS 30.0%
GTAAATGTAGAATTTCCTGA+TGG - chr2.1:63570534-63570553 MS.gene003126:CDS 30.0%
TACCAACAATGTTTCCATAA+GGG - chr2.1:63571642-63571661 MS.gene003126:CDS 30.0%
TGGACAATCTGTATAGAAAT+GGG - chr2.1:63571587-63571606 MS.gene003126:CDS 30.0%
TTGGACAATCTGTATAGAAA+TGG - chr2.1:63571586-63571605 MS.gene003126:CDS 30.0%
TTGTTTGACTAACGTAAATG+CGG + chr2.1:63570459-63570478 None:intergenic 30.0%
AATGATTGAGAGGATGGAAA+AGG - chr2.1:63570967-63570986 MS.gene003126:intron 35.0%
AATTGGTGGAAATGATTGAG+AGG - chr2.1:63570957-63570976 MS.gene003126:intron 35.0%
AGAGCAGATGCATAAAGAAT+TGG - chr2.1:63570940-63570959 MS.gene003126:intron 35.0%
GATAAAAGAAGAACTTACCC+GGG + chr2.1:63569950-63569969 None:intergenic 35.0%
GGATAAAAGAAGAACTTACC+CGG + chr2.1:63569951-63569970 None:intergenic 35.0%
GGTAGTGTAGTTGAATTTGA+TGG - chr2.1:63570735-63570754 MS.gene003126:intron 35.0%
TGGATCCAGCAAATAAAACA+TGG + chr2.1:63570582-63570601 None:intergenic 35.0%
TGTCCAATTCGTCAAAGTAA+GGG + chr2.1:63571573-63571592 None:intergenic 35.0%
TGTTTCCATAAGGGAAGAAA+AGG - chr2.1:63571651-63571670 MS.gene003126:CDS 35.0%
TGTTTGACTAACGTAAATGC+GGG + chr2.1:63570458-63570477 None:intergenic 35.0%
TTCCCTTATGGAAACATTGT+TGG + chr2.1:63571647-63571666 None:intergenic 35.0%
TTGCAGCAAATTTGGTAAAG+AGG - chr2.1:63570903-63570922 MS.gene003126:intron 35.0%
TTGTCCAATTCGTCAAAGTA+AGG + chr2.1:63571574-63571593 None:intergenic 35.0%
TTTCTGAATCAGCGATATGA+GGG + chr2.1:63571089-63571108 None:intergenic 35.0%
! GGAAGATTTTGCAGCAAATT+TGG - chr2.1:63570895-63570914 MS.gene003126:intron 35.0%
!! ATCGCTGATTCAGAAACTAT+TGG - chr2.1:63571093-63571112 MS.gene003126:intron 35.0%
!! TCGCTGATTCAGAAACTATT+GGG - chr2.1:63571094-63571113 MS.gene003126:intron 35.0%
!!! ACTAGCCATGTTTTATTTGC+TGG - chr2.1:63570574-63570593 MS.gene003126:intron 35.0%
AAGAGAAGAGGATGAAGTTG+AGG - chr2.1:63571159-63571178 MS.gene003126:intron 40.0%
AAGTACTATAGGAGGACAGT+TGG - chr2.1:63571511-63571530 MS.gene003126:CDS 40.0%
AATTCGGCTCAAGAAAGAGA+AGG - chr2.1:63570852-63570871 MS.gene003126:intron 40.0%
ACTACACTACCATGCAATAG+CGG + chr2.1:63570726-63570745 None:intergenic 40.0%
AGTACTATAGGAGGACAGTT+GGG - chr2.1:63571512-63571531 MS.gene003126:CDS 40.0%
ATAAGGGAAGAAAAGGAGCA+GGG - chr2.1:63571658-63571677 MS.gene003126:CDS 40.0%
ATCGAAAGGATCAATATGGG+AGG - chr2.1:63571411-63571430 MS.gene003126:intron 40.0%
ATGTAGAATTTCCTGATGGC+AGG - chr2.1:63570538-63570557 MS.gene003126:CDS 40.0%
GAACACAAGTTCGGTCATAT+GGG - chr2.1:63571388-63571407 MS.gene003126:intron 40.0%
GCAGATGCATAAAGAATTGG+TGG - chr2.1:63570943-63570962 MS.gene003126:intron 40.0%
GGATAAAGAGAGAAGAAGCA+TGG - chr2.1:63570993-63571012 MS.gene003126:intron 40.0%
GGGATCGAAAGGATCAATAT+GGG - chr2.1:63571408-63571427 MS.gene003126:intron 40.0%
GTTTCTGAATCAGCGATATG+AGG + chr2.1:63571090-63571109 None:intergenic 40.0%
TCATCATTTCAGCTCGTCTT+CGG - chr2.1:63570796-63570815 MS.gene003126:intron 40.0%
TCTGTATAGAAATGGGTCCT+TGG - chr2.1:63571594-63571613 MS.gene003126:CDS 40.0%
TGCATTTCCATTTCTCTCCA+AGG + chr2.1:63571614-63571633 None:intergenic 40.0%
TGGGATCGAAAGGATCAATA+TGG - chr2.1:63571407-63571426 MS.gene003126:intron 40.0%
TGTAGAATTTCCTGATGGCA+GGG - chr2.1:63570539-63570558 MS.gene003126:CDS 40.0%
TTGAGAGGATGGAAAAGGAA+AGG - chr2.1:63570972-63570991 MS.gene003126:intron 40.0%
TTGCCCTTACTTTGACGAAT+TGG - chr2.1:63571567-63571586 MS.gene003126:CDS 40.0%
! GAAGAAGTGCAAGGAGAAAT+GGG - chr2.1:63571477-63571496 MS.gene003126:CDS 40.0%
! TAGCCTTAATCCAGTACATG+GGG - chr2.1:63571257-63571276 MS.gene003126:intron 40.0%
!! CTAGCCTTAATCCAGTACAT+GGG - chr2.1:63571256-63571275 MS.gene003126:intron 40.0%
!!! GGGCAAGTTTTGCTATTAAC+AGG + chr2.1:63571553-63571572 None:intergenic 40.0%
AATGGGTCCTTGGAGAGAAA+TGG - chr2.1:63571604-63571623 MS.gene003126:CDS 45.0%
ACCGAACTTGTGTTCCAATG+CGG + chr2.1:63571383-63571402 None:intergenic 45.0%
ACCGCATTGGAACACAAGTT+CGG - chr2.1:63571379-63571398 MS.gene003126:intron 45.0%
ACCTTTATGGAAGGTGGTAG+AGG + chr2.1:63569872-63569891 None:intergenic 45.0%
AGCGCTTGTACTTCAACATC+TGG + chr2.1:63571346-63571365 None:intergenic 45.0%
AGGAAGCGGAATAAGAGAAG+AGG - chr2.1:63571147-63571166 MS.gene003126:intron 45.0%
CATAAGGGAAGAAAAGGAGC+AGG - chr2.1:63571657-63571676 MS.gene003126:CDS 45.0%
CATAGCAGTAAGAACCGCAT+TGG - chr2.1:63571366-63571385 MS.gene003126:intron 45.0%
CCATGTACTGGATTAAGGCT+AGG + chr2.1:63571258-63571277 None:intergenic 45.0%
CCTTTATGGAAGGTGGTAGA+GGG + chr2.1:63569871-63569890 None:intergenic 45.0%
CTTTATGGAAGGTGGTAGAG+GGG + chr2.1:63569870-63569889 None:intergenic 45.0%
GAGAAAGAGTTCGAGAAAGC+TGG - chr2.1:63570874-63570893 MS.gene003126:intron 45.0%
GCGATCCAAGTAACAGTAGA+TGG - chr2.1:63571320-63571339 MS.gene003126:intron 45.0%
GGAACACAAGTTCGGTCATA+TGG - chr2.1:63571387-63571406 MS.gene003126:intron 45.0%
GGAGATGGAGAGAATTAAGC+AGG - chr2.1:63571024-63571043 MS.gene003126:intron 45.0%
GGTGGAAATGATTGAGAGGA+TGG - chr2.1:63570961-63570980 MS.gene003126:intron 45.0%
GTAGAATTTCCTGATGGCAG+GGG - chr2.1:63570540-63570559 MS.gene003126:CDS 45.0%
GTCAATTAGCCGCTATTGCA+TGG - chr2.1:63570714-63570733 MS.gene003126:intron 45.0%
TCAATATGGGAGGAGATAGC+TGG - chr2.1:63571421-63571440 MS.gene003126:intron 45.0%
TCTGGCCATCTACTGTTACT+TGG + chr2.1:63571328-63571347 None:intergenic 45.0%
TTACCCGGGTTCTTGACAAA+GGG + chr2.1:63569936-63569955 None:intergenic 45.0%
TTCGGTCATATGGGATCGAA+AGG - chr2.1:63571397-63571416 MS.gene003126:intron 45.0%
! ACCTGATGGGGATGAGTTTT+TGG + chr2.1:63569799-63569818 None:intergenic 45.0%
! CCTGATGGGGATGAGTTTTT+GGG + chr2.1:63569798-63569817 None:intergenic 45.0%
! CGAAGAAGTGCAAGGAGAAA+TGG - chr2.1:63571476-63571495 MS.gene003126:CDS 45.0%
! CTGATGGGGATGAGTTTTTG+GGG + chr2.1:63569797-63569816 None:intergenic 45.0%
! CTGCTCCTTTTCTTCCCTTA+TGG + chr2.1:63571659-63571678 None:intergenic 45.0%
! TTGTTATTGCTCACCTCAGC+CGG + chr2.1:63571283-63571302 None:intergenic 45.0%
!! CCTAGCCTTAATCCAGTACA+TGG - chr2.1:63571255-63571274 MS.gene003126:intron 45.0%
!! GGATGAGTTTTTGGGGATTG+TGG + chr2.1:63569790-63569809 None:intergenic 45.0%
!! TGAGTTTTTGGGGATTGTGG+TGG + chr2.1:63569787-63569806 None:intergenic 45.0%
!! TTTTTGGGGATTGTGGTGGT+TGG + chr2.1:63569783-63569802 None:intergenic 45.0%
AAATGGAGCAACATGGTCGG+AGG + chr2.1:63569720-63569739 None:intergenic 50.0%
ACCACCTTCCATAAAGGTCG+AGG - chr2.1:63569874-63569893 MS.gene003126:CDS 50.0%
AGTCGGGAAATGGAGCAACA+TGG + chr2.1:63569727-63569746 None:intergenic 50.0%
CAAAGAAGAATGACCCGACC+CGG + chr2.1:63569912-63569931 None:intergenic 50.0%
CCCAAAAACTCATCCCCATC+AGG - chr2.1:63569795-63569814 MS.gene003126:CDS 50.0%
CCCTCTACCACCTTCCATAA+AGG - chr2.1:63569868-63569887 MS.gene003126:CDS 50.0%
CTGGATTAAGGCTAGGCTGT+TGG + chr2.1:63571251-63571270 None:intergenic 50.0%
CTTAATCCAGTACATGGGGC+CGG - chr2.1:63571261-63571280 MS.gene003126:intron 50.0%
CTTACCCGGGTTCTTGACAA+AGG + chr2.1:63569937-63569956 None:intergenic 50.0%
CTTATTCCGCTTCCTTCTGC+TGG + chr2.1:63571142-63571161 None:intergenic 50.0%
GGGAAATGGAGCAACATGGT+CGG + chr2.1:63569723-63569742 None:intergenic 50.0%
TACCCGGGTTCTTGACAAAG+GGG + chr2.1:63569935-63569954 None:intergenic 50.0%
TATAGGAGGACAGTTGGGAG+CGG - chr2.1:63571517-63571536 MS.gene003126:CDS 50.0%
TCCTCGACCTTTATGGAAGG+TGG + chr2.1:63569878-63569897 None:intergenic 50.0%
TTGCCCCTTTGTCAAGAACC+CGG - chr2.1:63569929-63569948 MS.gene003126:CDS 50.0%
TTTCCTGATGGCAGGGGTTT+CGG - chr2.1:63570546-63570565 MS.gene003126:CDS 50.0%
! TCGTTCTGCGAAGAAGTGCA+AGG - chr2.1:63571468-63571487 MS.gene003126:CDS 50.0%
!! GAGATAGCTGGAGCATTGCA+GGG - chr2.1:63571433-63571452 MS.gene003126:intron 50.0%
AAGAAGCTAGAGCGGCTGAG+AGG - chr2.1:63571050-63571069 MS.gene003126:intron 55.0%
AAGATAAGGTCGCCGGAGTC+GGG + chr2.1:63569743-63569762 None:intergenic 55.0%
AGACGGGTCCTCGACCTTTA+TGG + chr2.1:63569885-63569904 None:intergenic 55.0%
AGCTCGTCTTCGGATGACGT+GGG - chr2.1:63570806-63570825 MS.gene003126:intron 55.0%
ATTCCGCTTCCTTCTGCTGG+TGG + chr2.1:63571139-63571158 None:intergenic 55.0%
CAGATTCCTCCACCAGCAGA+AGG - chr2.1:63571127-63571146 MS.gene003126:intron 55.0%
CAGTTGGGAGCGGCAAGAAA+CGG - chr2.1:63571527-63571546 MS.gene003126:CDS 55.0%
CGGCCCCATGTACTGGATTA+AGG + chr2.1:63571263-63571282 None:intergenic 55.0%
GAAGATAAGGTCGCCGGAGT+CGG + chr2.1:63569744-63569763 None:intergenic 55.0%
GATTGTGGTGGTTGGAGAGG+AGG + chr2.1:63569775-63569794 None:intergenic 55.0%
GCAGGACGAAGAAGCTAGAG+CGG - chr2.1:63571042-63571061 MS.gene003126:intron 55.0%
GGGTCCTCGACCTTTATGGA+AGG + chr2.1:63569881-63569900 None:intergenic 55.0%
GTGTGGTGAGAAGGGAAACC+AGG + chr2.1:63569848-63569867 None:intergenic 55.0%
GTTGCTCCATTTCCCGACTC+CGG - chr2.1:63569728-63569747 MS.gene003126:CDS 55.0%
TGAGAAGGGAAACCAGGAGG+AGG + chr2.1:63569842-63569861 None:intergenic 55.0%
TGCCCCTTTGTCAAGAACCC+GGG - chr2.1:63569930-63569949 MS.gene003126:CDS 55.0%
TGGTGAGAAGGGAAACCAGG+AGG + chr2.1:63569845-63569864 None:intergenic 55.0%
! AGAAGAAGCATGGAAGCGCG+AGG - chr2.1:63571003-63571022 MS.gene003126:intron 55.0%
! GTAGAGGGGTGTGGTGAGAA+GGG + chr2.1:63569856-63569875 None:intergenic 55.0%
!! GGAGATAGCTGGAGCATTGC+AGG - chr2.1:63571432-63571451 MS.gene003126:intron 55.0%
AAACCAGGAGGAGGAGGAGG+AGG + chr2.1:63569833-63569852 None:intergenic 60.0%
AGGAGGAGGGATGGACCTGA+TGG + chr2.1:63569813-63569832 None:intergenic 60.0%
CACCCTTGACCCACAACGTG+GGG - chr2.1:63571203-63571222 MS.gene003126:intron 60.0%
CAGCTCGTCTTCGGATGACG+TGG - chr2.1:63570805-63570824 MS.gene003126:intron 60.0%
CCACCCTTGACCCACAACGT+GGG - chr2.1:63571202-63571221 MS.gene003126:intron 60.0%
CCACGTTGTGGGTCAAGGGT+GGG + chr2.1:63571204-63571223 None:intergenic 60.0%
CCTCCACCAGCAGAAGGAAG+CGG - chr2.1:63571133-63571152 MS.gene003126:intron 60.0%
CTTGACCCACAACGTGGGGT+CGG - chr2.1:63571207-63571226 MS.gene003126:intron 60.0%
GAAGGGAAACCAGGAGGAGG+AGG + chr2.1:63569839-63569858 None:intergenic 60.0%
GACCCCACGTTGTGGGTCAA+GGG + chr2.1:63571208-63571227 None:intergenic 60.0%
GAGGAGGGATGGACCTGATG+GGG + chr2.1:63569811-63569830 None:intergenic 60.0%
GATCCGAAACCCCTGCCATC+AGG + chr2.1:63570552-63570571 None:intergenic 60.0%
GGAGGAGGGATGGACCTGAT+GGG + chr2.1:63569812-63569831 None:intergenic 60.0%
GGGGATTGTGGTGGTTGGAG+AGG + chr2.1:63569778-63569797 None:intergenic 60.0%
TGGAAGGTGGTAGAGGGGTG+TGG + chr2.1:63569865-63569884 None:intergenic 60.0%
! AGCATGGAAGCGCGAGGAGA+TGG - chr2.1:63571009-63571028 MS.gene003126:intron 60.0%
! GGTAGAGGGGTGTGGTGAGA+AGG + chr2.1:63569857-63569876 None:intergenic 60.0%
!! AGCTGGAGCATTGCAGGGCA+TGG - chr2.1:63571438-63571457 MS.gene003126:CDS 60.0%
!! GCTGGAGCATTGCAGGGCAT+GGG - chr2.1:63571439-63571458 MS.gene003126:CDS 60.0%
AGGAGGAGGAGGAGGAGGGA+TGG + chr2.1:63569822-63569841 None:intergenic 65.0%
AGGTCGCCGGAGTCGGGAAA+TGG + chr2.1:63569737-63569756 None:intergenic 65.0%
CAACATGGTCGGAGGCGAGG+AGG + chr2.1:63569712-63569731 None:intergenic 65.0%
CCCACCCTTGACCCACAACG+TGG - chr2.1:63571201-63571220 MS.gene003126:intron 65.0%
CCCACGTTGTGGGTCAAGGG+TGG + chr2.1:63571205-63571224 None:intergenic 65.0%
CCGCTTCCTTCTGCTGGTGG+AGG + chr2.1:63571136-63571155 None:intergenic 65.0%
CGACCCCACGTTGTGGGTCA+AGG + chr2.1:63571209-63571228 None:intergenic 65.0%
CTCAGCCGACCCCACGTTGT+GGG + chr2.1:63571215-63571234 None:intergenic 65.0%
GAGCAACATGGTCGGAGGCG+AGG + chr2.1:63569715-63569734 None:intergenic 65.0%
GAGGAGGAGGAGGAGGAGGA+GGG + chr2.1:63569826-63569845 None:intergenic 65.0%
GCGCTGCTATTCGACGACGC+TGG + chr2.1:63570647-63570666 None:intergenic 65.0%
GGCGACCTTATCTTCGCCGC+CGG - chr2.1:63569749-63569768 MS.gene003126:CDS 65.0%
GGCGGCGAAGATAAGGTCGC+CGG + chr2.1:63569750-63569769 None:intergenic 65.0%
GGGAAACCAGGAGGAGGAGG+AGG + chr2.1:63569836-63569855 None:intergenic 65.0%
GTCGAGGACCCGTCTCGATC+CGG - chr2.1:63569890-63569909 MS.gene003126:CDS 65.0%
GTGGTGGTTGGAGAGGAGGC+CGG + chr2.1:63569771-63569790 None:intergenic 65.0%
TCGAGGACCCGTCTCGATCC+GGG - chr2.1:63569891-63569910 MS.gene003126:CDS 65.0%
TGACCCGACCCGGATCGAGA+CGG + chr2.1:63569902-63569921 None:intergenic 65.0%
CCACAACGTGGGGTCGGCTG+AGG - chr2.1:63571213-63571232 MS.gene003126:intron 70.0%
CCAGGAGGAGGAGGAGGAGG+AGG + chr2.1:63569830-63569849 None:intergenic 70.0%
CCAGTACATGGGGCCGGCTG+AGG - chr2.1:63571267-63571286 MS.gene003126:intron 70.0%
CCTCAGCCGACCCCACGTTG+TGG + chr2.1:63571216-63571235 None:intergenic 70.0%
CCTCAGCCGGCCCCATGTAC+TGG + chr2.1:63571270-63571289 None:intergenic 70.0%
CCTCCTCCTCCTCCTCCTCC+TGG - chr2.1:63569824-63569843 MS.gene003126:CDS 70.0%
GACCCGACCCGGATCGAGAC+GGG + chr2.1:63569901-63569920 None:intergenic 70.0%
GACCCGTCTCGATCCGGGTC+GGG - chr2.1:63569896-63569915 MS.gene003126:CDS 70.0%
GGACCCGTCTCGATCCGGGT+CGG - chr2.1:63569895-63569914 MS.gene003126:CDS 70.0%
GGAGGAGGAGGAGGAGGAGG+AGG + chr2.1:63569824-63569843 None:intergenic 70.0%
GGAGGCCGGCGGCGAAGATA+AGG + chr2.1:63569757-63569776 None:intergenic 70.0%
GTGGTTGGAGAGGAGGCCGG+CGG + chr2.1:63569768-63569787 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 63569695 63571690 63569695 ID=MS.gene003126
chr2.1 mRNA 63569695 63571690 63569695 ID=MS.gene003126.t1;Parent=MS.gene003126
chr2.1 exon 63571434 63571690 63571434 ID=MS.gene003126.t1.exon1;Parent=MS.gene003126.t1
chr2.1 CDS 63571434 63571690 63571434 ID=cds.MS.gene003126.t1;Parent=MS.gene003126.t1
chr2.1 exon 63569695 63570577 63569695 ID=MS.gene003126.t1.exon2;Parent=MS.gene003126.t1
chr2.1 CDS 63569695 63570577 63569695 ID=cds.MS.gene003126.t1;Parent=MS.gene003126.t1
Gene Sequence

>MS.gene003126

ATGGACAGCATGAACCTCCTCGCCTCCGACCATGTTGCTCCATTTCCCGACTCCGGCGACCTTATCTTCGCCGCCGGCCTCCTCTCCAACCACCACAATCCCCAAAAACTCATCCCCATCAGGTCCATCCCTCCTCCTCCTCCTCCTCCTCCTGGTTTCCCTTCTCACCACACCCCTCTACCACCTTCCATAAAGGTCGAGGACCCGTCTCGATCCGGGTCGGGTCATTCTTCTTTGCCCCTTTGTCAAGAACCCGGGTAAGTTCTTCTTTTATCCCTTAGCTTGTAAAGTTTTTACTTTTAAATTCATAAAGTTTTTATCTTTTTATCTTTTTTATTTTGGTTGAAAATTCAATGGTTAAACAATTAGGTTTAGTTTTAGTTCTCTCTGTTATTTTTTCAATTTTTTAATTCATTTTGAATCTGTATATGTTTGTTCAATTAAGCACGTTTGAAAAAAGCTAGAAAAAAGTGATTTTTAGTGAGAAATTTTGTTGCAGACAATTGTTGTTTATTTTACTGTCTTCATTGTAGATTTGTATCTTAATTCTTAACAAGATAATAGTAGAGAAATTGAACAATTTTTGTCAATTTTCATGGTGTGTTACAAATATGAAAATTAGTTACACTACTTTGTATGATTATATTTCTGATTGTAAATGTTTTGTTTTATGGTGCTGTGTTAGTGAAGAAAAAAGAATCATACAAGGTATTGTAGTATGAATTTGGATTTGGATTTGAATTTTCGATCATTGTGGAATCCCGCATTTACGTTAGTCAAACAATTAGGACTATTGGATCAATATTAAATGATTTGAGTGTTTAGATTGATTGACTGCAGTAAATGTAGAATTTCCTGATGGCAGGGGTTTCGGATCTAACTAGCCATGTTTTATTTGCTGGATCCAAGTTTTTATGTAGCATATATAATAGTTCATAAGGTAATAAATCCAGCGTCGTCGAATAGCAGCGCTGAATTTGAATAAATTGCTATTCTTCTGCTATTTAGTACAAAGTGTCGTCAATTAGCCGCTATTGCATGGTAGTGTAGTTGAATTTGATGGAATATTTCCTTTTACGATAATGATTCGAATTTGTGTTTTCATCATTTCAGCTCGTCTTCGGATGACGTGGGCAATTCATCTGCTAATACTAAAGAATTCGGCTCAAGAAAGAGAAGGAGAAAGAGTTCGAGAAAGCTGGAAGATTTTGCAGCAAATTTGGTAAAGAGGATGACGGAAAAGCAAGAGCAGATGCATAAAGAATTGGTGGAAATGATTGAGAGGATGGAAAAGGAAAGGATAAAGAGAGAAGAAGCATGGAAGCGCGAGGAGATGGAGAGAATTAAGCAGGACGAAGAAGCTAGAGCGGCTGAGAGGTCTCGTAGTTTAGCCCTCATATCGCTGATTCAGAAACTATTGGGTTATGAAATTCAGATTCCTCCACCAGCAGAAGGAAGCGGAATAAGAGAAGAGGATGAAGTTGAGGTGAACAACAACAACAACAGCCCACCCTTGACCCACAACGTGGGGTCGGCTGAGGTGAACAACAACACCAACAGCCTAGCCTTAATCCAGTACATGGGGCCGGCTGAGGTGAGCAATAACAAAAAACATTTCAACAGCGGCGATCCAAGTAACAGTAGATGGCCAGATGTTGAAGTACAAGCGCTCATAGCAGTAAGAACCGCATTGGAACACAAGTTCGGTCATATGGGATCGAAAGGATCAATATGGGAGGAGATAGCTGGAGCATTGCAGGGCATGGGATACACTCGTTCTGCGAAGAAGTGCAAGGAGAAATGGGAAAACATCAATAAGTACTATAGGAGGACAGTTGGGAGCGGCAAGAAACGGCCTGTTAATAGCAAAACTTGCCCTTACTTTGACGAATTGGACAATCTGTATAGAAATGGGTCCTTGGAGAGAAATGGAAATGCATTGAGCAATACCAACAATGTTTCCATAAGGGAAGAAAAGGAGCAGGGTGAAATTTGA
Protein sequence

>MS.gene003126.t1

MDSMNLLASDHVAPFPDSGDLIFAAGLLSNHHNPQKLIPIRSIPPPPPPPPGFPSHHTPLPPSIKVEDPSRSGSGHSSLPLCQEPGSSSDDVGNSSANTKEFGSRKRRRKSSRKLEDFAANLVKRMTEKQEQMHKELVEMIERMEKERIKREEAWKREEMERIKQDEEARAAERSRSLALISLIQKLLGYEIQIPPPAEGSGIREEDEVEVNNNNNSPPLTHNVGSAEVNNNTNSLALIQYMGPAEVSNNKKHFNSGDPSNSRWPDVEVQALIAVRTALEHKFGHMGSKGSIWEEIAGALQGMGYTRSAKKCKEKWENINKYYRRTVGSGKKRPVNSKTCPYFDELDNLYRNGSLERNGNALSNTNNVSIREEKEQGEI