AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003220.t1 MTR_6g051425 30.075 266 177 3 88 344 20 285 1.51e-37 144
MS.gene003220.t1 MTR_1g070355 29.682 283 179 10 44 314 34 308 2.82e-21 99.0
MS.gene003220.t1 MTR_4g067290 36.538 104 63 1 84 184 55 158 3.15e-15 76.3
MS.gene003220.t1 MTR_4g067290 36.538 104 63 1 84 184 55 158 3.33e-15 76.3
MS.gene003220.t1 MTR_4g067290 36.538 104 63 1 84 184 75 178 4.56e-15 76.3
MS.gene003220.t1 MTR_4g067290 36.538 104 63 1 84 184 75 178 4.58e-15 76.3
MS.gene003220.t1 MTR_4g067290 36.538 104 63 1 84 184 73 176 4.85e-15 76.3
MS.gene003220.t1 MTR_5g076070 26.408 284 174 10 43 309 17 282 4.99e-15 76.6
MS.gene003220.t1 MTR_4g067290 36.538 104 63 1 84 184 115 218 6.37e-15 76.6
MS.gene003220.t1 MTR_2g098920 34.014 147 90 3 78 219 75 219 1.11e-14 75.1
MS.gene003220.t1 MTR_0558s0020 25.843 267 148 10 91 320 42 295 3.93e-14 74.3
MS.gene003220.t1 MTR_7g446520 25.714 245 163 5 83 323 86 315 7.11e-14 73.9
MS.gene003220.t1 MTR_8g446020 28.649 185 110 6 148 320 81 255 7.28e-13 69.7
MS.gene003220.t1 MTR_2g098880 29.032 155 94 3 34 182 20 164 1.45e-12 68.6
MS.gene003220.t1 MTR_2g098880 29.032 155 94 3 34 182 32 176 1.87e-12 68.6
MS.gene003220.t1 MTR_7g446510 29.213 178 114 3 160 329 156 329 1.37e-11 67.0
MS.gene003220.t1 MTR_7g446480 28.736 174 120 2 160 329 141 314 5.49e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003220.t1 AT1G80010 53.528 652 286 7 64 703 74 720 0.0 696
MS.gene003220.t1 AT1G52520 50.147 678 297 13 48 704 42 699 0.0 644
MS.gene003220.t1 AT4G15090 33.387 620 398 6 84 697 50 660 8.62e-120 379
MS.gene003220.t1 AT4G15090 33.387 620 398 6 84 697 50 660 8.62e-120 379
MS.gene003220.t1 AT4G15090 33.387 620 398 6 84 697 50 660 8.62e-120 379
MS.gene003220.t1 AT4G15090 33.387 620 398 6 84 697 50 660 8.62e-120 379
MS.gene003220.t1 AT4G15090 33.387 620 398 6 84 697 50 660 8.62e-120 379
MS.gene003220.t1 AT1G76320 34.138 621 383 9 89 703 1 601 9.65e-112 353
MS.gene003220.t1 AT1G76320 34.138 621 383 9 89 703 1 601 4.03e-111 353
MS.gene003220.t1 AT1G76320 34.138 621 383 9 89 703 1 601 5.34e-111 353
MS.gene003220.t1 AT1G76320 34.138 621 383 9 89 703 1 601 5.34e-111 353
MS.gene003220.t1 AT3G22170 29.709 653 409 8 84 718 69 689 5.36e-104 337
MS.gene003220.t1 AT3G22170 29.709 653 409 8 84 718 69 689 5.36e-104 337
MS.gene003220.t1 AT4G38180 32.807 570 367 8 84 644 72 634 8.29e-103 333
MS.gene003220.t1 AT2G27110 29.438 676 418 15 36 704 16 639 6.79e-92 305
MS.gene003220.t1 AT2G27110 29.438 676 418 15 36 704 16 639 6.79e-92 305
MS.gene003220.t1 AT5G18960 29.272 632 403 9 70 700 196 784 4.59e-89 296
MS.gene003220.t1 AT5G18960 29.921 127 80 1 83 209 41 158 9.16e-13 72.4
MS.gene003220.t1 AT5G18960 29.272 632 403 9 70 700 196 784 4.59e-89 296
MS.gene003220.t1 AT5G18960 29.921 127 80 1 83 209 41 158 9.16e-13 72.4
MS.gene003220.t1 AT3G06250 28.769 650 411 9 58 700 156 760 2.14e-88 294
MS.gene003220.t1 AT3G06250 30.405 148 91 3 84 231 27 162 6.89e-13 72.8
MS.gene003220.t1 AT3G06250 28.769 650 411 9 58 700 156 760 2.14e-88 294
MS.gene003220.t1 AT3G06250 30.405 148 91 3 84 231 27 162 6.89e-13 72.8
MS.gene003220.t1 AT3G06250 28.769 650 411 9 58 700 156 760 2.14e-88 294
MS.gene003220.t1 AT3G06250 30.405 148 91 3 84 231 27 162 6.89e-13 72.8
MS.gene003220.t1 AT2G32250 31.362 558 331 7 88 641 41 550 5.76e-88 293
MS.gene003220.t1 AT2G32250 31.362 558 331 7 88 641 41 550 1.56e-87 293
MS.gene003220.t1 AT2G32250 31.362 558 331 7 88 641 41 550 1.56e-87 293
MS.gene003220.t1 AT2G32250 31.655 556 332 6 88 641 41 550 1.74e-87 292
MS.gene003220.t1 AT2G32250 31.655 556 332 6 88 641 41 550 1.74e-87 292
MS.gene003220.t1 AT2G32250 31.655 556 332 6 88 641 41 550 1.74e-87 292
MS.gene003220.t1 AT2G32250 31.239 557 331 7 89 641 1 509 5.10e-87 290
MS.gene003220.t1 AT2G32250 31.532 555 332 6 89 641 1 509 5.54e-87 290
MS.gene003220.t1 AT2G27110 32.438 484 306 7 228 704 25 494 3.09e-84 281
MS.gene003220.t1 AT4G19990 29.853 613 367 10 66 658 16 585 3.41e-83 280
MS.gene003220.t1 AT4G19990 30.236 592 350 10 87 658 23 571 3.78e-83 280
MS.gene003220.t1 AT4G19990 30.236 592 350 10 87 658 23 571 3.78e-83 280
MS.gene003220.t1 AT4G19990 30.236 592 350 10 87 658 23 571 4.20e-83 279
MS.gene003220.t1 AT4G19990 30.236 592 350 10 87 658 23 571 5.64e-83 280
MS.gene003220.t1 AT4G19990 30.236 592 350 10 87 658 23 571 5.64e-83 280
MS.gene003220.t1 AT4G19990 30.813 529 317 9 136 658 29 514 9.62e-76 258
MS.gene003220.t1 AT4G38170 30.786 471 297 9 256 715 19 471 1.04e-71 243
MS.gene003220.t1 AT1G10240 25.216 579 393 13 83 641 46 604 1.77e-52 193
MS.gene003220.t1 AT1G10240 25.216 579 393 13 83 641 46 604 1.77e-52 193
MS.gene003220.t1 AT5G28530 26.333 600 386 18 85 655 55 627 1.03e-45 174
MS.gene003220.t1 AT5G28530 26.000 600 384 18 85 655 55 623 5.96e-45 172
MS.gene003220.t1 AT5G28530 26.000 600 384 18 85 655 55 623 5.96e-45 172
MS.gene003220.t1 AT5G28530 26.190 336 218 9 327 655 252 564 7.79e-24 107
MS.gene003220.t1 AT5G28530 25.595 336 216 9 327 655 252 560 4.89e-23 104
MS.gene003220.t1 AT3G59470 37.037 108 65 1 84 188 69 176 3.96e-15 76.3
MS.gene003220.t1 AT3G59470 37.037 108 65 1 84 188 69 176 3.96e-15 76.3
MS.gene003220.t1 AT3G59470 37.037 108 65 1 84 188 81 188 4.46e-15 76.6

Find 153 sgRNAs with CRISPR-Local

Find 273 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGAAACCAAATAATGATTT+TGG 0.088603 2.1:+62290863 None:intergenic
GACAATTGAGGAACATATTT+TGG 0.117184 2.1:-62292064 MS.gene003220:CDS
GATATCCCAAAATCATTATT+TGG 0.175601 2.1:-62290869 MS.gene003220:CDS
TGGTTGTCTGGTGCAGTTTC+AGG 0.204396 2.1:-62291709 MS.gene003220:CDS
AATAGCTATGCCAGGGAAAT+TGG 0.208039 2.1:-62292605 MS.gene003220:CDS
AGGGCTGCATATAGTTATTT+TGG 0.210404 2.1:-62292026 MS.gene003220:CDS
AAAATCATTATTTGGTTTCT+TGG 0.223441 2.1:-62290861 MS.gene003220:CDS
TTTGGATTGATTCTAGTTCT+AGG 0.231462 2.1:-62292046 MS.gene003220:CDS
AACTGAAGCTCTTGATGATT+TGG 0.234723 2.1:-62291379 MS.gene003220:CDS
CGGTTTGTAACAATAGTTTC+TGG 0.236513 2.1:+62291819 None:intergenic
AATTAGGACTCACCAGTTTA+TGG 0.247549 2.1:+62292117 None:intergenic
GGGAAATTGGATTTGCTATT+CGG 0.274552 2.1:-62292592 MS.gene003220:CDS
ATTTATAGAGTCTTTGAATA+AGG 0.281326 2.1:-62290833 MS.gene003220:intron
TAGATGAATTTGAAAGAGAT+TGG 0.315956 2.1:-62291632 MS.gene003220:CDS
AGCAACAAGCCTTTATCATT+TGG 0.320082 2.1:+62294275 None:intergenic
AGTCGGCTCAACCTTTCTTT+TGG 0.323275 2.1:+62292310 None:intergenic
ACTGTAGATGCTTCCGGTTA+TGG 0.346656 2.1:-62292245 MS.gene003220:CDS
CTCTACTATTGCACATTCTA+TGG 0.347062 2.1:+62291788 None:intergenic
AAATGATCAAAACACTGAAC+TGG 0.347410 2.1:+62290928 None:intergenic
CTGTAGATGCTTCCGGTTAT+GGG 0.351570 2.1:-62292244 MS.gene003220:CDS
TTACCGTGATGTCGAGATTA+GGG 0.358736 2.1:+62294249 None:intergenic
GATGGTGGCGAACATGAAAA+TGG 0.368179 2.1:-62292746 MS.gene003220:CDS
CTCTGTCCTCGTGCAAGCTT+CGG 0.376248 2.1:+62291565 None:intergenic
GCACGAGGACAGAGAGCTTT+GGG 0.378976 2.1:-62291556 MS.gene003220:CDS
CTTCTCTTTACTGTTACGTT+TGG 0.380146 2.1:+62292553 None:intergenic
TTTACCGTGATGTCGAGATT+AGG 0.383499 2.1:+62294248 None:intergenic
TTACTTCTGCCACCATAAAC+TGG 0.384916 2.1:-62292129 MS.gene003220:CDS
GGAAATTGGATTTGCTATTC+GGG 0.388562 2.1:-62292591 MS.gene003220:CDS
TTGGATTGATTCTAGTTCTA+GGG 0.391970 2.1:-62292045 MS.gene003220:CDS
AAATGCCTATCATTTCTTAC+CGG 0.393224 2.1:+62291162 None:intergenic
TGAGAGTAGTGGAATCTAAT+AGG 0.393466 2.1:-62292427 MS.gene003220:CDS
ATATTTCGAGCAGGGTTTGA+TGG 0.395255 2.1:+62290555 None:intergenic
TTATTGCATAATGTTGGGTC+AGG 0.399471 2.1:+62291604 None:intergenic
TGCACGAGGACAGAGAGCTT+TGG 0.400964 2.1:-62291557 MS.gene003220:CDS
GATTTCTTATTGCATAATGT+TGG 0.401141 2.1:+62291598 None:intergenic
TGGAATTTCTAATTACGAAA+AGG 0.404269 2.1:-62291502 MS.gene003220:CDS
TCAAACTCCACTCCCTCAAC+AGG 0.406663 2.1:+62292656 None:intergenic
CTTGCAATAGACAAATTCTA+TGG 0.410394 2.1:+62291748 None:intergenic
AGAATTTGAGCTCCCATAAC+CGG 0.414377 2.1:+62292232 None:intergenic
GCGTGTTCTCCAAATGATAA+AGG 0.420925 2.1:-62294284 MS.gene003220:CDS
ATTTCTTATTGCATAATGTT+GGG 0.422728 2.1:+62291599 None:intergenic
ATGCCAACTGTAGATGCTTC+CGG 0.429407 2.1:-62292251 MS.gene003220:CDS
GGAATTTCTAATTACGAAAA+GGG 0.436213 2.1:-62291501 MS.gene003220:CDS
AATTTGTGAGACGCTGAAAC+AGG 0.436325 2.1:+62291239 None:intergenic
AATGTGTCCTTGGAGTAAAC+TGG 0.441443 2.1:+62291531 None:intergenic
GGTTTGTAACAATAGTTTCT+GGG 0.441868 2.1:+62291820 None:intergenic
CTCTTTCAGGATCAAATGAA+TGG 0.442050 2.1:+62292372 None:intergenic
ACTTCAAAGGCTACCTATGC+AGG 0.442185 2.1:-62291083 MS.gene003220:CDS
ATTCCACTAGTTGCATTTGT+TGG 0.443557 2.1:-62291957 MS.gene003220:CDS
AGATGAATTTGAAAGAGATT+GGG 0.444699 2.1:-62291631 MS.gene003220:CDS
GCAGATCATCCGTGGTTGTC+TGG 0.446361 2.1:-62291721 MS.gene003220:CDS
CTCCACTCCCTCAACAGGTT+GGG 0.449152 2.1:+62292661 None:intergenic
TGGAGAACACGCTGCATTGT+CGG 0.455406 2.1:+62294295 None:intergenic
TGTAATTGCTTTAACTTCAA+AGG 0.458366 2.1:-62291096 MS.gene003220:CDS
GATGCAGGTTGCAGCTGTTA+AGG 0.463308 2.1:-62290578 MS.gene003220:intron
AGAACATCAAGGGGAAGAAC+CGG 0.463849 2.1:-62291181 MS.gene003220:CDS
TAGTGGAATCTAATAGGTGG+AGG 0.476847 2.1:-62292421 MS.gene003220:CDS
ATAGCTATTATAATAGTTGT+AGG 0.483777 2.1:+62292619 None:intergenic
CTATTCGGGTTAAATCTTCG+TGG 0.484193 2.1:-62292577 MS.gene003220:CDS
CGCCCAACCTGTTGAGGGAG+TGG 0.485119 2.1:-62292663 MS.gene003220:CDS
TGCTCGAAATATGTGTTTGG+TGG 0.487448 2.1:-62290544 MS.gene003220:CDS
ACATCATCGCCACCTCATCT+TGG 0.488238 2.1:+62291265 None:intergenic
CCCTGCTCGAAATATGTGTT+TGG 0.491484 2.1:-62290547 MS.gene003220:CDS
GTCTTGTGTTGTAGTTGTGA+AGG 0.496440 2.1:-62292521 MS.gene003220:CDS
TAGAGAGTTGCAACTCATAG+TGG 0.499235 2.1:+62291319 None:intergenic
CGTAGGAAAGAAACAAGAAC+AGG 0.499540 2.1:-62292470 MS.gene003220:CDS
AGACATTTGAAACGTATACT+TGG 0.502054 2.1:-62291881 MS.gene003220:CDS
ATCCTTAATTACAATGGTGT+GGG 0.502114 2.1:-62291048 MS.gene003220:CDS
ACTATTATAATAGCTATGCC+AGG 0.505200 2.1:-62292613 MS.gene003220:CDS
TTATCATAATTCATGTATGT+AGG 0.507183 2.1:+62290520 None:intergenic
AAGAACCGGTAAGAAATGAT+AGG 0.508332 2.1:-62291167 MS.gene003220:CDS
ACCAAACACATATTTCGAGC+AGG 0.509508 2.1:+62290546 None:intergenic
CACATAGGAGGATGGACAGT+GGG 0.512891 2.1:-62292334 MS.gene003220:CDS
CAAATTCTATGGTGAGCCCT+CGG 0.514324 2.1:+62291759 None:intergenic
ATTACAAATGCAACGTACTT+CGG 0.514793 2.1:+62291113 None:intergenic
TACTATTTCCAGATAGATGA+GGG 0.522528 2.1:-62292851 MS.gene003220:intron
TAAGCAACTACTTGAGGTTG+AGG 0.526880 2.1:-62292816 MS.gene003220:CDS
GGACAGTGGGGCCAAAAGAA+AGG 0.531952 2.1:-62292321 MS.gene003220:CDS
ATACTTGGTTATTTAGAGCA+TGG 0.532023 2.1:-62291866 MS.gene003220:CDS
GGGAGCTCAAATTCTAATGA+AGG 0.533763 2.1:-62292224 MS.gene003220:CDS
ATCAAAACACTGAACTGGGT+TGG 0.536498 2.1:+62290933 None:intergenic
AGACTACGCCCAACCTGTTG+AGG 0.540785 2.1:-62292669 MS.gene003220:CDS
AAATTCTATGGTGAGCCCTC+GGG 0.542802 2.1:+62291760 None:intergenic
TATCCTTAATTACAATGGTG+TGG 0.549164 2.1:-62291049 MS.gene003220:CDS
ACTCCACTCCCTCAACAGGT+TGG 0.553844 2.1:+62292660 None:intergenic
GACTACGCCCAACCTGTTGA+GGG 0.556672 2.1:-62292668 MS.gene003220:CDS
GTGATGGATCTGAATGATGA+TGG 0.557426 2.1:-62292086 MS.gene003220:CDS
AATAGCAAATCCAATTTCCC+TGG 0.562021 2.1:+62292595 None:intergenic
CCAAACACATATTTCGAGCA+GGG 0.562427 2.1:+62290547 None:intergenic
TATACAAGGCATGCCTGCAT+AGG 0.564010 2.1:+62291070 None:intergenic
GGAAGATGAGGCATTTCAGA+TGG 0.566330 2.1:-62291688 MS.gene003220:CDS
TATTGCATAATGTTGGGTCA+GGG 0.567535 2.1:+62291605 None:intergenic
ACATGGTAAAAGAACATCAA+GGG 0.568150 2.1:-62291191 MS.gene003220:CDS
CGAGTCTATAGTGACATACA+TGG 0.575473 2.1:-62291208 MS.gene003220:CDS
TTGTATATCCTTAATTACAA+TGG 0.575751 2.1:-62291054 MS.gene003220:CDS
CACATTCTATGGTCTTACAT+CGG 0.577150 2.1:+62291799 None:intergenic
CTCTAAATCACATAGGAGGA+TGG 0.578313 2.1:-62292342 MS.gene003220:CDS
GTTTGTAACAATAGTTTCTG+GGG 0.579296 2.1:+62291821 None:intergenic
TTTGTAACAATAGTTTCTGG+GGG 0.582883 2.1:+62291822 None:intergenic
TTACTATTTCCAGATAGATG+AGG 0.583204 2.1:-62292852 MS.gene003220:intron
GTGGCGAACATGAAAATGGA+AGG 0.585092 2.1:-62292742 MS.gene003220:CDS
ACTACTTGAGGTTGAGGACA+AGG 0.585298 2.1:-62292810 MS.gene003220:CDS
AATGATCAAAACACTGAACT+GGG 0.585970 2.1:+62290929 None:intergenic
ACAAAGTAAGCAACTACTTG+AGG 0.589391 2.1:-62292822 MS.gene003220:CDS
TCCTTAATTACAATGGTGTG+GGG 0.589480 2.1:-62291047 MS.gene003220:CDS
ATAAAGAAGCTAATAGTCGT+AGG 0.594056 2.1:-62292487 MS.gene003220:CDS
AGTCAATTGTTTGAGATCGA+TGG 0.594570 2.1:-62292764 MS.gene003220:CDS
TACATGGTAAAAGAACATCA+AGG 0.596297 2.1:-62291192 MS.gene003220:CDS
ACACCAACAAATGCAACTAG+TGG 0.596327 2.1:+62291954 None:intergenic
TTGGGCTCCAGTTTACTCCA+AGG 0.596913 2.1:-62291538 MS.gene003220:CDS
AAAACTCTAAATCACATAGG+AGG 0.599156 2.1:-62292346 MS.gene003220:CDS
GCTCGAAATATGTGTTTGGT+GGG 0.599382 2.1:-62290543 MS.gene003220:CDS
CGACTCTAGATGTTGAAGTA+CGG 0.600367 2.1:-62292292 MS.gene003220:CDS
GGAATCTAATAGGTGGAGGG+TGG 0.600823 2.1:-62292417 MS.gene003220:CDS
CTTGACAAAATATAATGACA+TGG 0.602201 2.1:+62291018 None:intergenic
CAATTGTTTGAGATCGATGG+TGG 0.603449 2.1:-62292761 MS.gene003220:CDS
CAAGTCTTGCAGATCATCCG+TGG 0.605006 2.1:-62291729 MS.gene003220:CDS
ATTCAATAAGTTCCAAGATG+AGG 0.606495 2.1:-62291277 MS.gene003220:CDS
CATAGAATGTGCAATAGTAG+AGG 0.608377 2.1:-62291787 MS.gene003220:CDS
GGTATGCAAGTAGTTGAAGA+AGG 0.610848 2.1:-62290895 MS.gene003220:CDS
TAACCGGAAGCATCTACAGT+TGG 0.612183 2.1:+62292248 None:intergenic
ACTATGACTGTCAACTCCCG+TGG 0.616006 2.1:+62292712 None:intergenic
GAGTAGTGGAATCTAATAGG+TGG 0.616272 2.1:-62292424 MS.gene003220:CDS
AGCTCAAATTCTAATGAAGG+AGG 0.617343 2.1:-62292221 MS.gene003220:CDS
CAATAAGTTCCAAGATGAGG+TGG 0.622529 2.1:-62291274 MS.gene003220:CDS
CGCAAAACTCTAAATCACAT+AGG 0.623536 2.1:-62292349 MS.gene003220:CDS
GAGCCCTAATCTCGACATCA+CGG 0.626041 2.1:-62294252 MS.gene003220:intron
AATGGAAGGGAGGAAACCAC+GGG 0.626247 2.1:-62292728 MS.gene003220:CDS
GGTGCAGTTTCAGGAAGATG+AGG 0.635119 2.1:-62291700 MS.gene003220:CDS
AAATGGAAGGGAGGAAACCA+CGG 0.638573 2.1:-62292729 MS.gene003220:CDS
CCTTAATTACAATGGTGTGG+GGG 0.643743 2.1:-62291046 MS.gene003220:CDS
ATTGTAATTAAGGATATACA+AGG 0.644132 2.1:+62291056 None:intergenic
CATGGTAAAAGAACATCAAG+GGG 0.645016 2.1:-62291190 MS.gene003220:CDS
CGTACTTCAACATCTAGAGT+CGG 0.648024 2.1:+62292293 None:intergenic
CTATTATAATAGCTATGCCA+GGG 0.648310 2.1:-62292612 MS.gene003220:CDS
TTTGTTGGTGTTAATCACCA+TGG 0.653331 2.1:-62291942 MS.gene003220:CDS
ACATTGTCAGAGCTGAGATG+TGG 0.654932 2.1:+62290964 None:intergenic
TAGGACTCACCAGTTTATGG+TGG 0.657453 2.1:+62292120 None:intergenic
AGTGGAATCTAATAGGTGGA+GGG 0.661358 2.1:-62292420 MS.gene003220:CDS
TTTGATCATTTGTACAAACG+TGG 0.664936 2.1:-62290916 MS.gene003220:CDS
AATGATTAGACTGAGAGTAG+TGG 0.669263 2.1:-62292438 MS.gene003220:CDS
TGGCGAACATGAAAATGGAA+GGG 0.678993 2.1:-62292741 MS.gene003220:CDS
CGAACATGAAAATGGAAGGG+AGG 0.681120 2.1:-62292738 MS.gene003220:CDS
TTGTAACAATAGTTTCTGGG+GGG 0.684006 2.1:+62291823 None:intergenic
CTACTTGAGGTTGAGGACAA+GGG 0.687690 2.1:-62292809 MS.gene003220:CDS
AAAGCTCCGAAGCTTGCACG+AGG 0.688357 2.1:-62291571 MS.gene003220:CDS
CTAGCAAAACAGATTGACCA+TGG 0.689075 2.1:+62291925 None:intergenic
GAAACTGCACCAGACAACCA+CGG 0.692031 2.1:+62291712 None:intergenic
TCACATAGGAGGATGGACAG+TGG 0.701404 2.1:-62292335 MS.gene003220:CDS
ACTATTTCCAGATAGATGAG+GGG 0.701880 2.1:-62292850 MS.gene003220:intron
ACATAGGAGGATGGACAGTG+GGG 0.705660 2.1:-62292333 MS.gene003220:CDS
CGTAACAGTAAAGAGAAGCG+TGG 0.709182 2.1:-62292548 MS.gene003220:CDS
TGAATGATGATGGACAATTG+AGG 0.749302 2.1:-62292076 MS.gene003220:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATATTTTTTATTGATTAAC+AGG - chr2.1:62291159-62291178 MS.gene003220:CDS 10.0%
!!! ATATTGTTAACAATTTTAAA+GGG + chr2.1:62291420-62291439 None:intergenic 10.0%
!!! TATATTGTTAACAATTTTAA+AGG + chr2.1:62291421-62291440 None:intergenic 10.0%
!! ATAATCAGTGTTATAAATAA+AGG + chr2.1:62291614-62291633 None:intergenic 15.0%
!! ATTATTTCACATAATTACTT+TGG - chr2.1:62291136-62291155 MS.gene003220:CDS 15.0%
!! TGAAACTTATATTGTTAATA+TGG + chr2.1:62291481-62291500 None:intergenic 15.0%
!!! TATTGTTAACAATTTTAAAG+GGG + chr2.1:62291419-62291438 None:intergenic 15.0%
!! AAGATATAATGATAGTATAG+TGG - chr2.1:62291731-62291750 MS.gene003220:CDS 20.0%
!! AAGATTAAAAATTCAGAGTT+TGG + chr2.1:62290752-62290771 None:intergenic 20.0%
!! AGATATAATGATAGTATAGT+GGG - chr2.1:62291732-62291751 MS.gene003220:CDS 20.0%
!! AGCAATATAAAATTTCATGT+TGG - chr2.1:62291825-62291844 MS.gene003220:CDS 20.0%
!! ATAGCTATTATAATAGTTGT+AGG + chr2.1:62292193-62292212 None:intergenic 20.0%
!! ATTGTAATTAAGGATATACA+AGG + chr2.1:62293756-62293775 None:intergenic 20.0%
!! ATTTATAGAGTCTTTGAATA+AGG - chr2.1:62293976-62293995 MS.gene003220:intron 20.0%
!! ATTTCTTATTGCATAATGTT+GGG + chr2.1:62293213-62293232 None:intergenic 20.0%
!! CATGGTTACTTAAAATAAAA+GGG - chr2.1:62291017-62291036 MS.gene003220:CDS 20.0%
!! GCAATATAAAATTTCATGTT+GGG - chr2.1:62291826-62291845 MS.gene003220:CDS 20.0%
!! GTCATAACATAATATAATAG+CGG - chr2.1:62291542-62291561 MS.gene003220:CDS 20.0%
!! TCAAAGAGAAAAAAAATTGT+GGG - chr2.1:62291346-62291365 MS.gene003220:CDS 20.0%
!! TCATGGTTACTTAAAATAAA+AGG - chr2.1:62291016-62291035 MS.gene003220:CDS 20.0%
!! TGTTATGACATTGATATTAT+TGG + chr2.1:62291531-62291550 None:intergenic 20.0%
!! TGTTTGCAAATAAATAGTTT+GGG + chr2.1:62290685-62290704 None:intergenic 20.0%
!! TTATCATAATTCATGTATGT+AGG + chr2.1:62294292-62294311 None:intergenic 20.0%
!! TTCAAAGAGAAAAAAAATTG+TGG - chr2.1:62291345-62291364 MS.gene003220:CDS 20.0%
!! TTGTATATCCTTAATTACAA+TGG - chr2.1:62293755-62293774 MS.gene003220:intron 20.0%
!! TTGTTTGCAAATAAATAGTT+TGG + chr2.1:62290686-62290705 None:intergenic 20.0%
!!! AAAAATTTGAAGTGATAAAC+AGG + chr2.1:62291066-62291085 None:intergenic 20.0%
!!! AAAATCATTATTTGGTTTCT+TGG - chr2.1:62293948-62293967 MS.gene003220:intron 20.0%
!!! AAGAAACCAAATAATGATTT+TGG + chr2.1:62293949-62293968 None:intergenic 20.0%
!!! AGAAACCAAATAATGATTTT+GGG + chr2.1:62293948-62293967 None:intergenic 20.0%
!!! AGAATAATTGTTTTTGAAGA+AGG - chr2.1:62291185-62291204 MS.gene003220:CDS 20.0%
!!! CAAATTCATCTAATGTTTTA+GGG + chr2.1:62293169-62293188 None:intergenic 20.0%
!!! GAATAATTGTTTTTGAAGAA+GGG - chr2.1:62291186-62291205 MS.gene003220:CDS 20.0%
!!! TCAAATTCATCTAATGTTTT+AGG + chr2.1:62293170-62293189 None:intergenic 20.0%
!!! TGAAATTTTATATTGCTTAC+AGG + chr2.1:62291822-62291841 None:intergenic 20.0%
!!! TTTTAATGATAACACTGATA+GGG - chr2.1:62291372-62291391 MS.gene003220:CDS 20.0%
!!! TTTTTAATGATAACACTGAT+AGG - chr2.1:62291371-62291390 MS.gene003220:CDS 20.0%
! AACAATATAACCATGATTCA+AGG - chr2.1:62291432-62291451 MS.gene003220:CDS 25.0%
! AGAACAATTTATGTATCAGA+AGG + chr2.1:62294093-62294112 None:intergenic 25.0%
! AGATGAATTTGAAAGAGATT+GGG - chr2.1:62293178-62293197 MS.gene003220:intron 25.0%
! CTTGACAAAATATAATGACA+TGG + chr2.1:62293794-62293813 None:intergenic 25.0%
! GATATCCCAAAATCATTATT+TGG - chr2.1:62293940-62293959 MS.gene003220:intron 25.0%
! GATTTCTTATTGCATAATGT+TGG + chr2.1:62293214-62293233 None:intergenic 25.0%
! GGAATTTCTAATTACGAAAA+GGG - chr2.1:62293308-62293327 MS.gene003220:intron 25.0%
! GTGAAATAATCAATGTCATT+AGG + chr2.1:62291126-62291145 None:intergenic 25.0%
! TAGATGAATTTGAAAGAGAT+TGG - chr2.1:62293177-62293196 MS.gene003220:intron 25.0%
! TCCATCACATAGAAAAAATT+AGG + chr2.1:62292711-62292730 None:intergenic 25.0%
! TGGAATTTCTAATTACGAAA+AGG - chr2.1:62293307-62293326 MS.gene003220:intron 25.0%
! TTACTTAAAATAAAAGGGAG+TGG - chr2.1:62291022-62291041 MS.gene003220:CDS 25.0%
! TTTAATGATAACACTGATAG+GGG - chr2.1:62291373-62291392 MS.gene003220:CDS 25.0%
!! AAATAAATAGTTTGGGTTAG+AGG + chr2.1:62290678-62290697 None:intergenic 25.0%
!! ATAGAATGTGAATGTCTTTT+TGG - chr2.1:62294160-62294179 MS.gene003220:intron 25.0%
!! ATTATATTTTGTCAAGATGG+AGG - chr2.1:62293798-62293817 MS.gene003220:intron 25.0%
!! GTAAATCTTCATTTTATCCA+TGG - chr2.1:62290579-62290598 MS.gene003220:intron 25.0%
!! GTCATTATATTTTGTCAAGA+TGG - chr2.1:62293795-62293814 MS.gene003220:intron 25.0%
!! TGTAATTGCTTTAACTTCAA+AGG - chr2.1:62293713-62293732 MS.gene003220:intron 25.0%
!! TTTTGTTGAGAAAAGAAAAG+AGG - chr2.1:62290713-62290732 MS.gene003220:intron 25.0%
!!! ATCTATGATTCCCTTTTTTA+AGG - chr2.1:62293334-62293353 MS.gene003220:intron 25.0%
!!! TCCTAATTTTTTCTATGTGA+TGG - chr2.1:62292707-62292726 MS.gene003220:CDS 25.0%
!!! TCTATGATTCCCTTTTTTAA+GGG - chr2.1:62293335-62293354 MS.gene003220:intron 25.0%
!!! TCTGTTTTTTAAGTTCAAAG+TGG - chr2.1:62290902-62290921 MS.gene003220:CDS 25.0%
AAAACTCTAAATCACATAGG+AGG - chr2.1:62292463-62292482 MS.gene003220:CDS 30.0%
AAATGATCAAAACACTGAAC+TGG + chr2.1:62293884-62293903 None:intergenic 30.0%
AAATGCCTATCATTTCTTAC+CGG + chr2.1:62293650-62293669 None:intergenic 30.0%
AATATAACCATGATTCAAGG+CGG - chr2.1:62291435-62291454 MS.gene003220:CDS 30.0%
AATGATCAAAACACTGAACT+GGG + chr2.1:62293883-62293902 None:intergenic 30.0%
AATTTATGTATCAGAAGGCA+TGG + chr2.1:62294088-62294107 None:intergenic 30.0%
ACATGGTAAAAGAACATCAA+GGG - chr2.1:62293618-62293637 MS.gene003220:intron 30.0%
ACTATTATAATAGCTATGCC+AGG - chr2.1:62292196-62292215 MS.gene003220:CDS 30.0%
ATAAAGAAGCTAATAGTCGT+AGG - chr2.1:62292322-62292341 MS.gene003220:CDS 30.0%
ATCCTTAATTACAATGGTGT+GGG - chr2.1:62293761-62293780 MS.gene003220:intron 30.0%
ATGCTAATATGTTGTGCATA+AGG - chr2.1:62294042-62294061 MS.gene003220:intron 30.0%
ATTACAAATGCAACGTACTT+CGG + chr2.1:62293699-62293718 None:intergenic 30.0%
ATTCAATAAGTTCCAAGATG+AGG - chr2.1:62293532-62293551 MS.gene003220:intron 30.0%
CTATTATAATAGCTATGCCA+GGG - chr2.1:62292197-62292216 MS.gene003220:CDS 30.0%
CTTGCAATAGACAAATTCTA+TGG + chr2.1:62293064-62293083 None:intergenic 30.0%
GGTTTGTAACAATAGTTTCT+GGG + chr2.1:62292992-62293011 None:intergenic 30.0%
GTTTGTAACAATAGTTTCTG+GGG + chr2.1:62292991-62293010 None:intergenic 30.0%
TACATGGTAAAAGAACATCA+AGG - chr2.1:62293617-62293636 MS.gene003220:intron 30.0%
TACTATTTCCAGATAGATGA+GGG - chr2.1:62291958-62291977 MS.gene003220:CDS 30.0%
TATCCTTAATTACAATGGTG+TGG - chr2.1:62293760-62293779 MS.gene003220:intron 30.0%
TTACTATTTCCAGATAGATG+AGG - chr2.1:62291957-62291976 MS.gene003220:CDS 30.0%
TTTGATCATTTGTACAAACG+TGG - chr2.1:62293893-62293912 MS.gene003220:intron 30.0%
! AAACAGTGTTGTTAAATAGC+AGG - chr2.1:62291230-62291249 MS.gene003220:CDS 30.0%
! AGACATTTGAAACGTATACT+TGG - chr2.1:62292928-62292947 MS.gene003220:intron 30.0%
! ATACTTGGTTATTTAGAGCA+TGG - chr2.1:62292943-62292962 MS.gene003220:intron 30.0%
! ATTTATGTATCAGAAGGCAT+GGG + chr2.1:62294087-62294106 None:intergenic 30.0%
! GACAATTGAGGAACATATTT+TGG - chr2.1:62292745-62292764 MS.gene003220:CDS 30.0%
! TAATGATAGTATAGTGGGAA+AGG - chr2.1:62291737-62291756 MS.gene003220:CDS 30.0%
! TCTTCATTTTATCCATGGAA+TGG - chr2.1:62290584-62290603 MS.gene003220:intron 30.0%
! TTTGTAACAATAGTTTCTGG+GGG + chr2.1:62292990-62293009 None:intergenic 30.0%
!! TTGGATTGATTCTAGTTCTA+GGG - chr2.1:62292764-62292783 MS.gene003220:CDS 30.0%
!! TTTGGATTGATTCTAGTTCT+AGG - chr2.1:62292763-62292782 MS.gene003220:CDS 30.0%
!!! ATATAGTTATTTTGGTGACG+TGG - chr2.1:62292791-62292810 MS.gene003220:CDS 30.0%
AAGAACCGGTAAGAAATGAT+AGG - chr2.1:62293642-62293661 MS.gene003220:intron 35.0%
AATAGCAAATCCAATTTCCC+TGG + chr2.1:62292217-62292236 None:intergenic 35.0%
AATGATTAGACTGAGAGTAG+TGG - chr2.1:62292371-62292390 MS.gene003220:CDS 35.0%
AATGCATAAACCATAGTGGT+TGG - chr2.1:62294207-62294226 MS.gene003220:intron 35.0%
AATTAGGACTCACCAGTTTA+TGG + chr2.1:62292695-62292714 None:intergenic 35.0%
AATTTCATGTTGGGAACTGT+AGG - chr2.1:62291835-62291854 MS.gene003220:CDS 35.0%
ACAAAGTAAGCAACTACTTG+AGG - chr2.1:62291987-62292006 MS.gene003220:CDS 35.0%
ACTATTTCCAGATAGATGAG+GGG - chr2.1:62291959-62291978 MS.gene003220:CDS 35.0%
AGAGAATAAAGAGACGACTA+GGG - chr2.1:62291503-62291522 MS.gene003220:CDS 35.0%
AGCAACAAGCCTTTATCATT+TGG + chr2.1:62290537-62290556 None:intergenic 35.0%
AGCTCAAATTCTAATGAAGG+AGG - chr2.1:62292588-62292607 MS.gene003220:CDS 35.0%
AGGAGACTGAAGCTAAAAAA+AGG - chr2.1:62292636-62292655 MS.gene003220:CDS 35.0%
AGTCATTAAGTTCCATTCCA+TGG + chr2.1:62290599-62290618 None:intergenic 35.0%
ATACAGGATCATGATTGTGT+AGG - chr2.1:62291773-62291792 MS.gene003220:CDS 35.0%
ATCATGAGGAACATCCATAA+CGG + chr2.1:62291665-62291684 None:intergenic 35.0%
ATGCACATGACCCTTAAAAA+AGG + chr2.1:62293348-62293367 None:intergenic 35.0%
ATTCCACTAGTTGCATTTGT+TGG - chr2.1:62292852-62292871 MS.gene003220:intron 35.0%
CATAGAATGTGCAATAGTAG+AGG - chr2.1:62293022-62293041 MS.gene003220:intron 35.0%
CATGGGTGAACAAAAATGAA+TGG + chr2.1:62294070-62294089 None:intergenic 35.0%
CATGGTAAAAGAACATCAAG+GGG - chr2.1:62293619-62293638 MS.gene003220:intron 35.0%
CATTGTTTGTGTTAGCCATT+AGG - chr2.1:62291088-62291107 MS.gene003220:CDS 35.0%
CATTTCCAACTATGACATTG+TGG - chr2.1:62290822-62290841 MS.gene003220:intron 35.0%
CGCAAAACTCTAAATCACAT+AGG - chr2.1:62292460-62292479 MS.gene003220:CDS 35.0%
CGGTTTGTAACAATAGTTTC+TGG + chr2.1:62292993-62293012 None:intergenic 35.0%
CGTTAGTGTGAGATAAATGA+GGG - chr2.1:62290968-62290987 MS.gene003220:CDS 35.0%
CTCCAAGTTCTGTGAAAAAA+TGG - chr2.1:62290927-62290946 MS.gene003220:CDS 35.0%
CTCTACTATTGCACATTCTA+TGG + chr2.1:62293024-62293043 None:intergenic 35.0%
CTCTTTCAGGATCAAATGAA+TGG + chr2.1:62292440-62292459 None:intergenic 35.0%
CTTCTCTTTACTGTTACGTT+TGG + chr2.1:62292259-62292278 None:intergenic 35.0%
GATCAATGCATAAACCATAG+TGG - chr2.1:62294203-62294222 MS.gene003220:intron 35.0%
GTTGGAAATGCAAATCAATC+AGG + chr2.1:62290812-62290831 None:intergenic 35.0%
TACAGGATCATGATTGTGTA+GGG - chr2.1:62291774-62291793 MS.gene003220:CDS 35.0%
TATTGCATAATGTTGGGTCA+GGG + chr2.1:62293207-62293226 None:intergenic 35.0%
TCCTTAATTACAATGGTGTG+GGG - chr2.1:62293762-62293781 MS.gene003220:intron 35.0%
TCGTTAGTGTGAGATAAATG+AGG - chr2.1:62290967-62290986 MS.gene003220:CDS 35.0%
TGCACATGACCCTTAAAAAA+GGG + chr2.1:62293347-62293366 None:intergenic 35.0%
TTATTGCATAATGTTGGGTC+AGG + chr2.1:62293208-62293227 None:intergenic 35.0%
! AACTGAAGCTCTTGATGATT+TGG - chr2.1:62293430-62293449 MS.gene003220:intron 35.0%
! AGGGCTGCATATAGTTATTT+TGG - chr2.1:62292783-62292802 MS.gene003220:CDS 35.0%
! AGTCAATTGTTTGAGATCGA+TGG - chr2.1:62292045-62292064 MS.gene003220:CDS 35.0%
! CACATTCTATGGTCTTACAT+CGG + chr2.1:62293013-62293032 None:intergenic 35.0%
! TATAGTGGGAAAGGTGAAAA+CGG - chr2.1:62291746-62291765 MS.gene003220:CDS 35.0%
! TGAGAGTAGTGGAATCTAAT+AGG - chr2.1:62292382-62292401 MS.gene003220:CDS 35.0%
! TTATGCGTTTTGCGAATTTC+TGG - chr2.1:62291567-62291586 MS.gene003220:CDS 35.0%
! TTGTAACAATAGTTTCTGGG+GGG + chr2.1:62292989-62293008 None:intergenic 35.0%
!! GGAAATTGGATTTGCTATTC+GGG - chr2.1:62292218-62292237 MS.gene003220:CDS 35.0%
!! GGGAAATTGGATTTGCTATT+CGG - chr2.1:62292217-62292236 MS.gene003220:CDS 35.0%
!! TGAATGATGATGGACAATTG+AGG - chr2.1:62292733-62292752 MS.gene003220:CDS 35.0%
!! TTTGTTGGTGTTAATCACCA+TGG - chr2.1:62292867-62292886 MS.gene003220:intron 35.0%
!!! CACCATTTTTTCACAGAACT+TGG + chr2.1:62290932-62290951 None:intergenic 35.0%
!!! TTTTTTTAGCTTCAGTCTCC+TGG + chr2.1:62292637-62292656 None:intergenic 35.0%
AAATAAAAGGGAGTGGTGCA+TGG - chr2.1:62291029-62291048 MS.gene003220:CDS 40.0%
AACCATGACTGCAAGACAAT+TGG + chr2.1:62291004-62291023 None:intergenic 40.0%
AACTATATCTTCCACACGAG+TGG + chr2.1:62291697-62291716 None:intergenic 40.0%
AATAGCTATGCCAGGGAAAT+TGG - chr2.1:62292204-62292223 MS.gene003220:CDS 40.0%
AATGTGTCCTTGGAGTAAAC+TGG + chr2.1:62293281-62293300 None:intergenic 40.0%
AATTTGTGAGACGCTGAAAC+AGG + chr2.1:62293573-62293592 None:intergenic 40.0%
ACACCAACAAATGCAACTAG+TGG + chr2.1:62292858-62292877 None:intergenic 40.0%
ACCAAACACATATTTCGAGC+AGG + chr2.1:62294266-62294285 None:intergenic 40.0%
ACTTGCCACAATGTCATAGT+TGG + chr2.1:62290830-62290849 None:intergenic 40.0%
AGAATTTGAGCTCCCATAAC+CGG + chr2.1:62292580-62292599 None:intergenic 40.0%
AGTGGAATCTAATAGGTGGA+GGG - chr2.1:62292389-62292408 MS.gene003220:CDS 40.0%
ATATTTCGAGCAGGGTTTGA+TGG + chr2.1:62294257-62294276 None:intergenic 40.0%
ATCAAAACACTGAACTGGGT+TGG + chr2.1:62293879-62293898 None:intergenic 40.0%
ATCACAACCAAGCACAAAGA+TGG + chr2.1:62291861-62291880 None:intergenic 40.0%
ATGGATGTTCCTCATGATCT+TGG - chr2.1:62291667-62291686 MS.gene003220:CDS 40.0%
ATTTGATCCGCCTTGAATCA+TGG + chr2.1:62291445-62291464 None:intergenic 40.0%
CAATAAGTTCCAAGATGAGG+TGG - chr2.1:62293535-62293554 MS.gene003220:intron 40.0%
CAGAGAATAAAGAGACGACT+AGG - chr2.1:62291502-62291521 MS.gene003220:CDS 40.0%
CCAAACACATATTTCGAGCA+GGG + chr2.1:62294265-62294284 None:intergenic 40.0%
CCTTAATTACAATGGTGTGG+GGG - chr2.1:62293763-62293782 MS.gene003220:intron 40.0%
CGACTCTAGATGTTGAAGTA+CGG - chr2.1:62292517-62292536 MS.gene003220:CDS 40.0%
CGAGTCTATAGTGACATACA+TGG - chr2.1:62293601-62293620 MS.gene003220:intron 40.0%
CGTAGGAAAGAAACAAGAAC+AGG - chr2.1:62292339-62292358 MS.gene003220:CDS 40.0%
CTAGCAAAACAGATTGACCA+TGG + chr2.1:62292887-62292906 None:intergenic 40.0%
CTCTAAATCACATAGGAGGA+TGG - chr2.1:62292467-62292486 MS.gene003220:CDS 40.0%
GCGTGTTCTCCAAATGATAA+AGG - chr2.1:62290525-62290544 MS.gene003220:CDS 40.0%
GCTCGAAATATGTGTTTGGT+GGG - chr2.1:62294266-62294285 MS.gene003220:CDS 40.0%
GGGAGCTCAAATTCTAATGA+AGG - chr2.1:62292585-62292604 MS.gene003220:CDS 40.0%
GGTATGCAAGTAGTTGAAGA+AGG - chr2.1:62293914-62293933 MS.gene003220:intron 40.0%
GTCTTGTGTTGTAGTTGTGA+AGG - chr2.1:62292288-62292307 MS.gene003220:CDS 40.0%
GTTAGCCATTAGGATGTCAT+AGG - chr2.1:62291098-62291117 MS.gene003220:CDS 40.0%
TAAGCAACTACTTGAGGTTG+AGG - chr2.1:62291993-62292012 MS.gene003220:CDS 40.0%
TAGAGAGTTGCAACTCATAG+TGG + chr2.1:62293493-62293512 None:intergenic 40.0%
TAGTGGAATCTAATAGGTGG+AGG - chr2.1:62292388-62292407 MS.gene003220:CDS 40.0%
TCCAGCAAAAAATGTGTCCT+TGG + chr2.1:62293291-62293310 None:intergenic 40.0%
TCGTGTGGAAGATATAGTTG+TGG - chr2.1:62291698-62291717 MS.gene003220:CDS 40.0%
TGCTATGCTGAAACTGTATG+CGG - chr2.1:62291277-62291296 MS.gene003220:CDS 40.0%
TGCTCGAAATATGTGTTTGG+TGG - chr2.1:62294265-62294284 MS.gene003220:CDS 40.0%
TGGCGAACATGAAAATGGAA+GGG - chr2.1:62292068-62292087 MS.gene003220:CDS 40.0%
TTACCGTGATGTCGAGATTA+GGG + chr2.1:62290563-62290582 None:intergenic 40.0%
TTACTTCTGCCACCATAAAC+TGG - chr2.1:62292680-62292699 MS.gene003220:CDS 40.0%
TTTACCGTGATGTCGAGATT+AGG + chr2.1:62290564-62290583 None:intergenic 40.0%
! AATAGTAGAGGTTTTCCCGA+GGG - chr2.1:62293034-62293053 MS.gene003220:intron 40.0%
! ATAGTGGGAAAGGTGAAAAC+GGG - chr2.1:62291747-62291766 MS.gene003220:CDS 40.0%
! CAATCTGTTTTGCTAGGATG+TGG - chr2.1:62292891-62292910 MS.gene003220:intron 40.0%
! CAATTGTTTGAGATCGATGG+TGG - chr2.1:62292048-62292067 MS.gene003220:CDS 40.0%
! CATGGTCAATCTGTTTTGCT+AGG - chr2.1:62292885-62292904 MS.gene003220:intron 40.0%
! CGTACTTCAACATCTAGAGT+CGG + chr2.1:62292519-62292538 None:intergenic 40.0%
! CTATTCGGGTTAAATCTTCG+TGG - chr2.1:62292232-62292251 MS.gene003220:CDS 40.0%
! GAGTAGTGGAATCTAATAGG+TGG - chr2.1:62292385-62292404 MS.gene003220:CDS 40.0%
! GCAACAACGTTAAGTTTTCC+AGG - chr2.1:62292616-62292635 MS.gene003220:CDS 40.0%
! TGTGCTTTTCAGTCTGATGT+TGG - chr2.1:62290629-62290648 MS.gene003220:intron 40.0%
! TTAGAGTTTTGCGCTCTTTC+AGG + chr2.1:62292453-62292472 None:intergenic 40.0%
! TTTTCAGTCTGATGTTGGAC+TGG - chr2.1:62290634-62290653 MS.gene003220:intron 40.0%
!! GTGATGGATCTGAATGATGA+TGG - chr2.1:62292723-62292742 MS.gene003220:CDS 40.0%
!!! TCCAAGGACACATTTTTTGC+TGG - chr2.1:62293287-62293306 MS.gene003220:intron 40.0%
!!! TGCGAATTTCTGGTGTTTTG+CGG - chr2.1:62291577-62291596 MS.gene003220:CDS 40.0%
AAATGGAAGGGAGGAAACCA+CGG - chr2.1:62292080-62292099 MS.gene003220:CDS 45.0%
AAATTCTATGGTGAGCCCTC+GGG + chr2.1:62293052-62293071 None:intergenic 45.0%
ACATTGTCAGAGCTGAGATG+TGG + chr2.1:62293848-62293867 None:intergenic 45.0%
ACTACTTGAGGTTGAGGACA+AGG - chr2.1:62291999-62292018 MS.gene003220:CDS 45.0%
ACTGTAGATGCTTCCGGTTA+TGG - chr2.1:62292564-62292583 MS.gene003220:CDS 45.0%
ACTTCAAAGGCTACCTATGC+AGG - chr2.1:62293726-62293745 MS.gene003220:intron 45.0%
AGAACATCAAGGGGAAGAAC+CGG - chr2.1:62293628-62293647 MS.gene003220:intron 45.0%
AGGCACCTATGACATCCTAA+TGG + chr2.1:62291106-62291125 None:intergenic 45.0%
AGGTGAAAACGGGTAGATAC+AGG - chr2.1:62291757-62291776 MS.gene003220:CDS 45.0%
ATATCCCCTATTGCCGATCT+GGG + chr2.1:62291317-62291336 None:intergenic 45.0%
ATGCCAACTGTAGATGCTTC+CGG - chr2.1:62292558-62292577 MS.gene003220:CDS 45.0%
CAAATTCTATGGTGAGCCCT+CGG + chr2.1:62293053-62293072 None:intergenic 45.0%
CACCAATTGTCTTGCAGTCA+TGG - chr2.1:62290999-62291018 MS.gene003220:CDS 45.0%
CCCCCACACCATTGTAATTA+AGG + chr2.1:62293766-62293785 None:intergenic 45.0%
CCCTGCTCGAAATATGTGTT+TGG - chr2.1:62294262-62294281 MS.gene003220:CDS 45.0%
CGAACATGAAAATGGAAGGG+AGG - chr2.1:62292071-62292090 MS.gene003220:CDS 45.0%
CGTAACAGTAAAGAGAAGCG+TGG - chr2.1:62292261-62292280 MS.gene003220:CDS 45.0%
CTACTTGAGGTTGAGGACAA+GGG - chr2.1:62292000-62292019 MS.gene003220:CDS 45.0%
CTGTAGATGCTTCCGGTTAT+GGG - chr2.1:62292565-62292584 MS.gene003220:CDS 45.0%
GGTTCGTTGTGTAACAGACA+AGG - chr2.1:62293997-62294016 MS.gene003220:intron 45.0%
GTGGCGAACATGAAAATGGA+AGG - chr2.1:62292067-62292086 MS.gene003220:CDS 45.0%
TAGGACTCACCAGTTTATGG+TGG + chr2.1:62292692-62292711 None:intergenic 45.0%
TATACAAGGCATGCCTGCAT+AGG + chr2.1:62293742-62293761 None:intergenic 45.0%
TATATCCCCTATTGCCGATC+TGG + chr2.1:62291318-62291337 None:intergenic 45.0%
TGCTATTCCCAATCCCAGAT+CGG - chr2.1:62291301-62291320 MS.gene003220:CDS 45.0%
! CAATAGTAGAGGTTTTCCCG+AGG - chr2.1:62293033-62293052 MS.gene003220:intron 45.0%
! GATGGTGGCGAACATGAAAA+TGG - chr2.1:62292063-62292082 MS.gene003220:CDS 45.0%
! GGAAGATGAGGCATTTCAGA+TGG - chr2.1:62293121-62293140 MS.gene003220:intron 45.0%
!! AGTCGGCTCAACCTTTCTTT+TGG + chr2.1:62292502-62292521 None:intergenic 45.0%
!! TAACCGGAAGCATCTACAGT+TGG + chr2.1:62292564-62292583 None:intergenic 45.0%
!!! TGGCATCTTGTTTTCAGCTG+TGG + chr2.1:62293473-62293492 None:intergenic 45.0%
AATGGAAGGGAGGAAACCAC+GGG - chr2.1:62292081-62292100 MS.gene003220:CDS 50.0%
ACATAGGAGGATGGACAGTG+GGG - chr2.1:62292476-62292495 MS.gene003220:CDS 50.0%
ACATCATCGCCACCTCATCT+TGG + chr2.1:62293547-62293566 None:intergenic 50.0%
ACCTGCATCACCAACCACTA+TGG + chr2.1:62294220-62294239 None:intergenic 50.0%
ACTATGACTGTCAACTCCCG+TGG + chr2.1:62292100-62292119 None:intergenic 50.0%
AGATCGATGCCAGTCCGTTA+TGG - chr2.1:62291648-62291667 MS.gene003220:CDS 50.0%
AGTGGACAGCCAAGATCATG+AGG + chr2.1:62291679-62291698 None:intergenic 50.0%
CAAGTCTTGCAGATCATCCG+TGG - chr2.1:62293080-62293099 MS.gene003220:intron 50.0%
CAATCCCAGATCGGCAATAG+GGG - chr2.1:62291310-62291329 MS.gene003220:CDS 50.0%
CACATAGGAGGATGGACAGT+GGG - chr2.1:62292475-62292494 MS.gene003220:CDS 50.0%
CCAATCCCAGATCGGCAATA+GGG - chr2.1:62291309-62291328 MS.gene003220:CDS 50.0%
CTGTAGGCCATCTTTGTGCT+TGG - chr2.1:62291851-62291870 MS.gene003220:CDS 50.0%
GAAACTGCACCAGACAACCA+CGG + chr2.1:62293100-62293119 None:intergenic 50.0%
GAGCCCTAATCTCGACATCA+CGG - chr2.1:62290557-62290576 MS.gene003220:CDS 50.0%
GATGCAGGTTGCAGCTGTTA+AGG - chr2.1:62294231-62294250 MS.gene003220:intron 50.0%
GGAATCTAATAGGTGGAGGG+TGG - chr2.1:62292392-62292411 MS.gene003220:CDS 50.0%
GGTGCAGTTTCAGGAAGATG+AGG - chr2.1:62293109-62293128 MS.gene003220:intron 50.0%
GTAGAGACCCCTCATCTATC+TGG + chr2.1:62291969-62291988 None:intergenic 50.0%
TCAAACTCCACTCCCTCAAC+AGG + chr2.1:62292156-62292175 None:intergenic 50.0%
TCACATAGGAGGATGGACAG+TGG - chr2.1:62292474-62292493 MS.gene003220:CDS 50.0%
TGGAGAACACGCTGCATTGT+CGG + chr2.1:62290517-62290536 None:intergenic 50.0%
TGGTTGTCTGGTGCAGTTTC+AGG - chr2.1:62293100-62293119 MS.gene003220:intron 50.0%
TTGGGCTCCAGTTTACTCCA+AGG - chr2.1:62293271-62293290 MS.gene003220:intron 50.0%
! CCTATTGCCGATCTGGGATT+GGG + chr2.1:62291311-62291330 None:intergenic 50.0%
!! ACCATAGTGGTTGGTGATGC+AGG - chr2.1:62294216-62294235 MS.gene003220:intron 50.0%
!! GAGGAACATCCATAACGGAC+TGG + chr2.1:62291660-62291679 None:intergenic 50.0%
AAAGCTCCGAAGCTTGCACG+AGG - chr2.1:62293238-62293257 MS.gene003220:intron 55.0%
ACTCCACTCCCTCAACAGGT+TGG + chr2.1:62292152-62292171 None:intergenic 55.0%
AGACTACGCCCAACCTGTTG+AGG - chr2.1:62292140-62292159 MS.gene003220:CDS 55.0%
ATCTTGGCTGTCCACTCGTG+TGG - chr2.1:62291683-62291702 MS.gene003220:CDS 55.0%
CCCAATCCCAGATCGGCAAT+AGG - chr2.1:62291308-62291327 MS.gene003220:CDS 55.0%
CTCCACTCCCTCAACAGGTT+GGG + chr2.1:62292151-62292170 None:intergenic 55.0%
CTCTGTCCTCGTGCAAGCTT+CGG + chr2.1:62293247-62293266 None:intergenic 55.0%
GACTACGCCCAACCTGTTGA+GGG - chr2.1:62292141-62292160 MS.gene003220:CDS 55.0%
GCACGAGGACAGAGAGCTTT+GGG - chr2.1:62293253-62293272 MS.gene003220:intron 55.0%
GCAGATCATCCGTGGTTGTC+TGG - chr2.1:62293088-62293107 MS.gene003220:intron 55.0%
GGACAGTGGGGCCAAAAGAA+AGG - chr2.1:62292488-62292507 MS.gene003220:CDS 55.0%
TGCACGAGGACAGAGAGCTT+TGG - chr2.1:62293252-62293271 MS.gene003220:intron 55.0%
! CCCTATTGCCGATCTGGGAT+TGG + chr2.1:62291312-62291331 None:intergenic 55.0%
CGCCCAACCTGTTGAGGGAG+TGG - chr2.1:62292146-62292165 MS.gene003220:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 62290510 62294321 62290510 ID=MS.gene003220
chr2.1 mRNA 62290510 62294321 62290510 ID=MS.gene003220.t1;Parent=MS.gene003220
chr2.1 exon 62294253 62294321 62294253 ID=MS.gene003220.t1.exon1;Parent=MS.gene003220.t1
chr2.1 CDS 62294253 62294321 62294253 ID=cds.MS.gene003220.t1;Parent=MS.gene003220.t1
chr2.1 exon 62290834 62292861 62290834 ID=MS.gene003220.t1.exon2;Parent=MS.gene003220.t1
chr2.1 CDS 62290834 62292861 62290834 ID=cds.MS.gene003220.t1;Parent=MS.gene003220.t1
chr2.1 exon 62290510 62290593 62290510 ID=MS.gene003220.t1.exon3;Parent=MS.gene003220.t1
chr2.1 CDS 62290510 62290593 62290510 ID=cds.MS.gene003220.t1;Parent=MS.gene003220.t1
Gene Sequence

>MS.gene003220

ATGACCGACAATGCAGCGTGTTCTCCAAATGATAAAGGCTTGTTGCTGAGCCCTAATCTCGACATCACGGTAAATCTTCATTTTATCCATGGAATGGAACTTAATGACTTGTTTTATGCTGTGCTTTTCAGTCTGATGTTGGACTGGATCTTATTTGTCTATCTGCCTCTAACCCAAACTATTTATTTGCAAACAACTCACGCTTTTGTTGAGAAAAGAAAAGAGGAATCAAATTAACGCCAAACTCTGAATTTTTAATCTTTTAAGACTTTAGTGTACTGTATAGTTTTGCTAAATTTCCTGATTGATTTGCATTTCCAACTATGACATTGTGGCAAGTCTTGAAGAACACGACACTCGATCTTTCTCGTATTGAGATACTTTTGTAATTTTCTGTTTTTTAAGTTCAAAGTGGTTCTCCAAGTTCTGTGAAAAAATGGTGCAATGTACAATTTTTTCGTTAGTGTGAGATAAATGAGGGAAGAAGTGCACCAATTGTCTTGCAGTCATGGTTACTTAAAATAAAAGGGAGTGGTGCATGGATTTTAATTATCCTGTTTATCACTTCAAATTTTTGTCATTGTTTGTGTTAGCCATTAGGATGTCATAGGTGCCTAATGACATTGATTATTTCACATAATTACTTTGGAATATTTTTTATTGATTAACAGGCATAGAATAATTGTTTTTGAAGAAGGGCATAGAATAATTGTTGATTATAAACAGTGTTGTTAAATAGCAGGATATGAACAAAAAACTATGAAAACTGCTATGCTGAAACTGTATGCGGCTGCTATTCCCAATCCCAGATCGGCAATAGGGGATATAGCATTAATTCAAAGAGAAAAAAAATTGTGGGTTTTTTTAATGATAACACTGATAGGGGAGCATGTGAGCTGCACATTACCCCTTTAAAATTGTTAACAATATAACCATGATTCAAGGCGGATCAAATCGTGCTCTTGATACCATATTAACAATATAAGTTTCAACAGAGAATAAAGAGACGACTAGGGTTCCAATAATATCAATGTCATAACATAATATAATAGCGGATTTATGCGTTTTGCGAATTTCTGGTGTTTTGCGGATTTCTGTTATCCTTTATTTATAACACTGATTATAAATCATTTATCTGAGATCGATGCCAGTCCGTTATGGATGTTCCTCATGATCTTGGCTGTCCACTCGTGTGGAAGATATAGTTGTGGTATTGCTTCAAAGATATAATGATAGTATAGTGGGAAAGGTGAAAACGGGTAGATACAGGATCATGATTGTGTAGGGTGAAAATAAAAGAAACTTCACACCTGTAAGCAATATAAAATTTCATGTTGGGAACTGTAGGCCATCTTTGTGCTTGGTTGTGATATTTCAAGTTCTGTTGTCAAATTCAATGCATACTCAAATTTCTTAGACTGTGTTTAGAATTTGTTTTTTTATTTCATTACTATTTCCAGATAGATGAGGGGTCTCTACAAAGTAAGCAACTACTTGAGGTTGAGGACAAGGGCAACGAGCTTGAGAATGACTGTAGTCAATTGTTTGAGATCGATGGTGGCGAACATGAAAATGGAAGGGAGGAAACCACGGGAGTTGACAGTCATAGTGAAGAATCTCAAAGAACAAAAGACTACGCCCAACCTGTTGAGGGAGTGGAGTTTGAAACTTATGATGATGCCTACAACTATTATAATAGCTATGCCAGGGAAATTGGATTTGCTATTCGGGTTAAATCTTCGTGGACCAAACGTAACAGTAAAGAGAAGCGTGGTGCTGTCTTGTGTTGTAGTTGTGAAGGTTTCAAAACAAATAAAGAAGCTAATAGTCGTAGGAAAGAAACAAGAACAGGCTGTCTAGCAATGATTAGACTGAGAGTAGTGGAATCTAATAGGTGGAGGGTGGATGAAGTCAAGCTTGAGCATAACCATTCATTTGATCCTGAAAGAGCGCAAAACTCTAAATCACATAGGAGGATGGACAGTGGGGCCAAAAGAAAGGTTGAGCCGACTCTAGATGTTGAAGTACGGACAATCAAGTTATATCGAATGCCAACTGTAGATGCTTCCGGTTATGGGAGCTCAAATTCTAATGAAGGAGGAACTAGCAACAACGTTAAGTTTTCCAGGAGACTGAAGCTAAAAAAAGGTGATGCAGAACTTATTTCAAATTACTTCTGCCACCATAAACTGGTGAGTCCTAATTTTTTCTATGTGATGGATCTGAATGATGATGGACAATTGAGGAACATATTTTGGATTGATTCTAGTTCTAGGGCTGCATATAGTTATTTTGGTGACGTGGTTGCATTTGACTCAACATATTTGTCAAACAATTATGAGATTCCACTAGTTGCATTTGTTGGTGTTAATCACCATGGTCAATCTGTTTTGCTAGGATGTGGTCTGCTTGCAGATGAGACATTTGAAACGTATACTTGGTTATTTAGAGCATGGCTTTCTTGTATGTCTAGTCGCCCCCCAGAAACTATTGTTACAAACCGATGTAAGACCATAGAATGTGCAATAGTAGAGGTTTTCCCGAGGGCTCACCATAGAATTTGTCTATTGCAAGTCTTGCAGATCATCCGTGGTTGTCTGGTGCAGTTTCAGGAAGATGAGGCATTTCAGATGGCACTGACAAGAGTGATATATGACCCTAAAACATTAGATGAATTTGAAAGAGATTGGGATTCCCTGACCCAACATTATGCAATAAGAAATCATGCAAAGCTCCGAAGCTTGCACGAGGACAGAGAGCTTTGGGCTCCAGTTTACTCCAAGGACACATTTTTTGCTGGAATTTCTAATTACGAAAAGGGTGAATCTATGATTCCCTTTTTTAAGGGTCATGTGCATCAACAAACTTCTTTGAAAGAATTTTTTGAGATTTATGAATTAGTTCAACAAAAAAAGCAAAAAACTGAAGCTCTTGATGATTTGGAGTCACAAAATTCAAGTCCACAGCTGAAAACAAGATGCCACTATGAGTTGCAACTCTCTAAATTGTACACGAATGCAATATTCAATAAGTTCCAAGATGAGGTGGCGATGATGTCTTCCTGTTTCAGCGTCTCACAAATTCAAACAAACGAGTCTATAGTGACATACATGGTAAAAGAACATCAAGGGGAAGAACCGGTAAGAAATGATAGGCATTTTGAAGTCATGTATGATAAAGCATTAACCGAAGTACGTTGCATTTGTAATTGCTTTAACTTCAAAGGCTACCTATGCAGGCATGCCTTGTATATCCTTAATTACAATGGTGTGGGGGAAATTCCATGTCATTATATTTTGTCAAGATGGAGGAAAGATTTTAAGCGATTGTATGTACCACATCTCAGCTCTGACAATGTTGATATTACCAACCCAGTTCAGTGTTTTGATCATTTGTACAAACGTGGTATGCAAGTAGTTGAAGAAGGCACGATATCCCAAAATCATTATTTGGTTTCTTGGCAGACATTTATAGAGTCTTTGAATAAGGTTCGTTGTGTAACAGACAAGGTTGAATAAATAAATCAGAAATAATGCTAATATGTTGTGCATAAGGTACCATTCATTTTTGTTCACCCATGCCTTCTGATACATAAATTGTTCTCTTATAGTTATAGTTTATGTTAAAAGTTTGCAGAATTATGTGAAAGCATAGAATGTGAATGTCTTTTTGGTTCGTTTACTAAATCAATTTGATCAATGCATAAACCATAGTGGTTGGTGATGCAGGTTGCAGCTGTTAAGGCCATCAAACCCTGCTCGAAATATGTGTTTGGTGGGCCTACATACATGAATTATGATAAAGAGAAATAG
Protein sequence

>MS.gene003220.t1

MTDNAACSPNDKGLLLSPNLDITIDEGSLQSKQLLEVEDKGNELENDCSQLFEIDGGEHENGREETTGVDSHSEESQRTKDYAQPVEGVEFETYDDAYNYYNSYAREIGFAIRVKSSWTKRNSKEKRGAVLCCSCEGFKTNKEANSRRKETRTGCLAMIRLRVVESNRWRVDEVKLEHNHSFDPERAQNSKSHRRMDSGAKRKVEPTLDVEVRTIKLYRMPTVDASGYGSSNSNEGGTSNNVKFSRRLKLKKGDAELISNYFCHHKLVSPNFFYVMDLNDDGQLRNIFWIDSSSRAAYSYFGDVVAFDSTYLSNNYEIPLVAFVGVNHHGQSVLLGCGLLADETFETYTWLFRAWLSCMSSRPPETIVTNRCKTIECAIVEVFPRAHHRICLLQVLQIIRGCLVQFQEDEAFQMALTRVIYDPKTLDEFERDWDSLTQHYAIRNHAKLRSLHEDRELWAPVYSKDTFFAGISNYEKGESMIPFFKGHVHQQTSLKEFFEIYELVQQKKQKTEALDDLESQNSSPQLKTRCHYELQLSKLYTNAIFNKFQDEVAMMSSCFSVSQIQTNESIVTYMVKEHQGEEPVRNDRHFEVMYDKALTEVRCICNCFNFKGYLCRHALYILNYNGVGEIPCHYILSRWRKDFKRLYVPHLSSDNVDITNPVQCFDHLYKRGMQVVEEGTISQNHYLVSWQTFIESLNKVAAVKAIKPCSKYVFGGPTYMNYDKEK