AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene003221

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003221.t1 MTR_6g051425 29.341 334 198 8 39 344 1 324 2.22e-34 134
MS.gene003221.t1 MTR_4g067290 35.644 101 62 1 65 162 56 156 2.49e-14 73.6
MS.gene003221.t1 MTR_4g067290 35.644 101 62 1 65 162 56 156 2.58e-14 73.6
MS.gene003221.t1 MTR_4g067290 35.644 101 62 1 65 162 74 174 3.02e-14 73.6
MS.gene003221.t1 MTR_4g067290 35.644 101 62 1 65 162 76 176 3.06e-14 73.6
MS.gene003221.t1 MTR_4g067290 35.644 101 62 1 65 162 76 176 3.34e-14 73.6
MS.gene003221.t1 MTR_4g067290 35.644 101 62 1 65 162 116 216 6.51e-14 73.6
MS.gene003221.t1 MTR_2g098880 35.849 106 58 2 65 162 73 176 1.31e-12 68.9
MS.gene003221.t1 MTR_2g098880 35.849 106 58 2 65 162 61 164 1.31e-12 68.9
MS.gene003221.t1 MTR_2g098920 34.653 101 63 1 65 162 80 180 1.37e-12 68.9
MS.gene003221.t1 MTR_7g446520 26.160 237 157 6 69 300 92 315 9.93e-12 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene003221.t1 AT1G52520 62.798 629 221 6 59 682 79 699 0.0 849
MS.gene003221.t1 AT1G80010 47.634 634 311 9 63 681 93 720 0.0 583
MS.gene003221.t1 AT4G15090 33.968 630 397 8 65 685 51 670 4.84e-121 380
MS.gene003221.t1 AT4G15090 33.968 630 397 8 65 685 51 670 4.84e-121 380
MS.gene003221.t1 AT4G15090 33.968 630 397 8 65 685 51 670 4.84e-121 380
MS.gene003221.t1 AT4G15090 33.968 630 397 8 65 685 51 670 4.84e-121 380
MS.gene003221.t1 AT4G15090 33.968 630 397 8 65 685 51 670 4.84e-121 380
MS.gene003221.t1 AT3G22170 33.480 681 386 12 21 675 29 668 1.37e-116 369
MS.gene003221.t1 AT3G22170 33.480 681 386 12 21 675 29 668 1.37e-116 369
MS.gene003221.t1 AT1G76320 35.135 629 356 13 69 677 1 597 1.68e-109 346
MS.gene003221.t1 AT1G76320 35.135 629 356 13 69 677 1 597 6.43e-109 346
MS.gene003221.t1 AT1G76320 35.135 629 356 13 69 677 1 597 6.47e-109 346
MS.gene003221.t1 AT1G76320 35.135 629 356 13 69 677 1 597 6.47e-109 346
MS.gene003221.t1 AT4G19990 34.106 604 333 9 39 622 1 559 3.92e-102 329
MS.gene003221.t1 AT4G19990 34.106 604 333 9 39 622 1 559 3.92e-102 329
MS.gene003221.t1 AT4G19990 34.106 604 333 9 39 622 1 559 7.83e-102 328
MS.gene003221.t1 AT4G19990 34.106 604 333 9 39 622 1 559 8.20e-102 328
MS.gene003221.t1 AT4G19990 34.106 604 333 9 39 622 1 559 8.20e-102 328
MS.gene003221.t1 AT4G19990 34.276 601 330 9 42 622 18 573 1.16e-101 328
MS.gene003221.t1 AT4G38180 32.278 632 399 13 65 678 73 693 7.20e-100 324
MS.gene003221.t1 AT4G19990 35.478 513 288 7 115 622 28 502 4.95e-93 303
MS.gene003221.t1 AT3G06250 30.189 636 385 13 51 678 176 760 2.40e-89 295
MS.gene003221.t1 AT3G06250 32.692 104 61 1 65 168 28 122 4.27e-11 66.6
MS.gene003221.t1 AT3G06250 30.189 636 385 13 51 678 176 760 2.40e-89 295
MS.gene003221.t1 AT3G06250 32.692 104 61 1 65 168 28 122 4.27e-11 66.6
MS.gene003221.t1 AT3G06250 30.189 636 385 13 51 678 176 760 2.40e-89 295
MS.gene003221.t1 AT3G06250 32.692 104 61 1 65 168 28 122 4.27e-11 66.6
MS.gene003221.t1 AT2G27110 29.042 637 414 11 49 682 38 639 4.62e-89 296
MS.gene003221.t1 AT2G27110 29.042 637 414 11 49 682 38 639 4.62e-89 296
MS.gene003221.t1 AT5G18960 31.018 619 377 15 65 678 211 784 2.87e-87 290
MS.gene003221.t1 AT5G18960 31.018 619 377 15 65 678 211 784 2.87e-87 290
MS.gene003221.t1 AT2G27110 30.189 477 323 4 207 682 27 494 7.11e-80 268
MS.gene003221.t1 AT2G32250 38.421 380 223 5 242 619 180 550 8.48e-78 264
MS.gene003221.t1 AT2G32250 38.421 380 223 5 242 619 139 509 1.15e-77 264
MS.gene003221.t1 AT2G32250 38.421 380 223 5 242 619 139 509 1.46e-77 264
MS.gene003221.t1 AT2G32250 38.421 380 223 5 242 619 180 550 1.80e-77 264
MS.gene003221.t1 AT2G32250 38.421 380 223 5 242 619 180 550 1.80e-77 264
MS.gene003221.t1 AT2G32250 38.421 380 223 5 242 619 180 550 1.89e-77 264
MS.gene003221.t1 AT2G32250 38.421 380 223 5 242 619 180 550 1.89e-77 264
MS.gene003221.t1 AT2G32250 38.421 380 223 5 242 619 180 550 1.89e-77 264
MS.gene003221.t1 AT4G38170 31.180 449 291 8 235 678 21 456 4.22e-72 244
MS.gene003221.t1 AT1G10240 26.121 647 414 16 65 680 48 661 2.01e-54 198
MS.gene003221.t1 AT1G10240 26.121 647 414 16 65 680 48 661 2.01e-54 198
MS.gene003221.t1 AT5G28530 26.121 647 425 18 64 681 54 676 3.52e-47 177
MS.gene003221.t1 AT5G28530 25.966 647 422 18 64 681 54 672 1.42e-46 176
MS.gene003221.t1 AT5G28530 25.966 647 422 18 64 681 54 672 1.42e-46 176
MS.gene003221.t1 AT5G28530 28.312 385 246 11 304 681 252 613 1.25e-30 128
MS.gene003221.t1 AT5G28530 28.052 385 243 11 304 681 252 609 6.07e-30 125
MS.gene003221.t1 AT3G59470 37.624 101 60 1 65 162 70 170 5.05e-14 73.2
MS.gene003221.t1 AT3G59470 37.624 101 60 1 65 162 70 170 5.05e-14 73.2
MS.gene003221.t1 AT3G59470 37.624 101 60 1 65 162 82 182 5.46e-14 73.2

Find 118 sgRNAs with CRISPR-Local

Find 161 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACATTTGAGGAATGTATTA+TGG 0.176053 2.1:-62288476 MS.gene003221:CDS
CATGGTCAATCTGTGTTATT+AGG 0.198497 2.1:-62288336 MS.gene003221:CDS
CGTCGACGGTGTCAAGCTTT+TGG 0.198914 2.1:+62289495 None:intergenic
CCCAGATCTCATCATTGTTT+TGG 0.201438 2.1:-62288171 MS.gene003221:CDS
CAATGGTCTAATCAGTTATT+TGG 0.205211 2.1:-62287331 MS.gene003221:CDS
ATGAACTCGGTCTTCATATA+GGG 0.286766 2.1:+62287972 None:intergenic
GAAGAAATGTTCTCTTGTTT+TGG 0.291820 2.1:-62287664 MS.gene003221:CDS
TCCATCAAATAAAAGAAGTT+TGG 0.301368 2.1:+62288513 None:intergenic
AACTTGTCCTTTATGTATAC+TGG 0.304465 2.1:+62287943 None:intergenic
CTGCAGTTCAAAAGAGCATT+TGG 0.320519 2.1:+62287738 None:intergenic
ACGACATAGGAAATTATAAA+TGG 0.326434 2.1:+62288551 None:intergenic
TTAGGTTGTGGGTTGCTTGC+AGG 0.332899 2.1:-62288318 MS.gene003221:CDS
TACATAAAGGACAAGTTTCT+TGG 0.335040 2.1:-62287937 MS.gene003221:CDS
AATGAACTCGGTCTTCATAT+AGG 0.343025 2.1:+62287971 None:intergenic
TGGAAGTGCCTTACAAGTTA+TGG 0.355565 2.1:-62287311 MS.gene003221:CDS
TGGCTCCATCATAATCTTCT+TGG 0.357834 2.1:-62287620 MS.gene003221:CDS
CACACAAAATCCCACCTCTT+TGG 0.362616 2.1:+62288998 None:intergenic
CATGGCAGGACAACCAGTTC+TGG 0.364832 2.1:+62288860 None:intergenic
CTCGAACATAACCACGTCTT+AGG 0.365078 2.1:-62288786 MS.gene003221:CDS
ACATTTGAGGAATGTATTAT+GGG 0.365589 2.1:-62288475 MS.gene003221:CDS
GCCAAAACAATGATGAGATC+TGG 0.365658 2.1:+62288170 None:intergenic
TTCTCCTCAAACTATTATTA+CGG 0.379468 2.1:-62288235 MS.gene003221:CDS
GAATTTCCCGCACCTGCTCT+TGG 0.386074 2.1:-62289071 MS.gene003221:CDS
CCATTACGATGCAGCGCGTC+AGG 0.390140 2.1:-62287266 MS.gene003221:CDS
CAATCTGTGTTATTAGGTTG+TGG 0.392493 2.1:-62288330 MS.gene003221:CDS
GGGAAACAGACAGGAGATAA+AGG 0.399801 2.1:-62287575 MS.gene003221:CDS
ATTGAAAGTGATTGAATTTG+AGG 0.404070 2.1:-62288052 MS.gene003221:CDS
GTTCAGACATACACCTTTGA+AGG 0.415617 2.1:-62287857 MS.gene003221:CDS
TTGATGGATTTCAATGATGA+AGG 0.429652 2.1:-62288498 MS.gene003221:CDS
ATTACCTGGCATTCTCCATC+GGG 0.434341 2.1:+62289134 None:intergenic
ACATGGGTTACTTGTATGTC+AGG 0.436090 2.1:-62288261 MS.gene003221:CDS
AAAATAACGACATGCAGCTT+TGG 0.449608 2.1:+62288440 None:intergenic
AATCTGTGTTATTAGGTTGT+GGG 0.451302 2.1:-62288329 MS.gene003221:CDS
TATATGAAGACCGAGTTCAT+TGG 0.452679 2.1:-62287969 MS.gene003221:CDS
GATTGAATTTGAGGCGGCAT+GGG 0.459082 2.1:-62288043 MS.gene003221:CDS
AGTTGTGATCAGGAATATAA+AGG 0.460169 2.1:+62287383 None:intergenic
CATGATAAGGATTAAGTTAG+TGG 0.463158 2.1:-62288841 MS.gene003221:CDS
TTGAGAAAAGAAACCAGAAC+TGG 0.466660 2.1:-62288873 MS.gene003221:CDS
ATATGAAGACCGAGTTCATT+GGG 0.473754 2.1:-62287968 MS.gene003221:CDS
AGCTCTGTCATCTGTTGCAT+TGG 0.476249 2.1:+62288623 None:intergenic
TTCTGCAATTGCACTTTGCA+AGG 0.480040 2.1:+62288200 None:intergenic
TCCCGCACCTGCTCTTGGAA+TGG 0.481909 2.1:-62289066 MS.gene003221:CDS
CTGGTTGTCCTGCCATGATA+AGG 0.483669 2.1:-62288854 MS.gene003221:CDS
CCAAAACAATGATGAGATCT+GGG 0.492319 2.1:+62288171 None:intergenic
GTGCTTTGTTGTAGCAGTCA+AGG 0.493576 2.1:-62288924 MS.gene003221:CDS
TCCCTCCTCCATAACTTGTA+AGG 0.494681 2.1:+62287303 None:intergenic
AAACGACCGAATCGTATTCA+TGG 0.494788 2.1:-62288293 MS.gene003221:CDS
AATTACCTGGCATTCTCCAT+CGG 0.497269 2.1:+62289133 None:intergenic
TTTGACCAAGAAGATTATGA+TGG 0.497729 2.1:+62287615 None:intergenic
GAACTACCAGAGTTGTGATC+AGG 0.499730 2.1:+62287373 None:intergenic
TGTTCTTGTTCTTCTTCTTG+AGG 0.500286 2.1:+62289461 None:intergenic
ATGTATTATGGGTTGATGCC+AGG 0.501404 2.1:-62288464 MS.gene003221:CDS
TTTGATTGAGGGAAACAGAC+AGG 0.503735 2.1:-62287584 MS.gene003221:CDS
CATGAAGTTCCAACTTGAAG+TGG 0.504481 2.1:-62287686 MS.gene003221:CDS
GGACAAGTTTCTTGGTGGAA+TGG 0.506245 2.1:-62287929 MS.gene003221:CDS
TTGGGCACCAGTATACATAA+AGG 0.506630 2.1:-62287950 MS.gene003221:CDS
AATAAGTTGAACCTAAGAAA+AGG 0.515686 2.1:-62288585 MS.gene003221:CDS
TGATTGAATTTGAGGCGGCA+TGG 0.516224 2.1:-62288044 MS.gene003221:CDS
ACATTTCTTCCACTTCAAGT+TGG 0.516480 2.1:+62287677 None:intergenic
AACTTAATCCTTATCATGGC+AGG 0.517948 2.1:+62288846 None:intergenic
TTCATGGTTGTTTAGAACAT+GGG 0.523651 2.1:-62288277 MS.gene003221:CDS
GTTGCCTAGCTCTGATGCGG+AGG 0.527630 2.1:-62288709 MS.gene003221:CDS
TTCCATTCCAAGAGCAGGTG+CGG 0.528014 2.1:+62289064 None:intergenic
ATGATATTGAACGTATTCAA+TGG 0.532271 2.1:-62287348 MS.gene003221:CDS
ATTCATGGTTGTTTAGAACA+TGG 0.534938 2.1:-62288278 MS.gene003221:CDS
ATAAAGGACAAGTTTCTTGG+TGG 0.535782 2.1:-62287934 MS.gene003221:CDS
CACTAACTTAATCCTTATCA+TGG 0.537066 2.1:+62288842 None:intergenic
TCCATTCCAAGAGCAGGTGC+GGG 0.537722 2.1:+62289065 None:intergenic
GTGATAGATACAGAAGGTAA+TGG 0.538353 2.1:-62288657 MS.gene003221:CDS
TTGCCTAGCTCTGATGCGGA+GGG 0.538891 2.1:-62288708 MS.gene003221:CDS
TATATTCCTGATCACAACTC+TGG 0.540777 2.1:-62287379 MS.gene003221:CDS
TCAAATTCCATTCCAAGAGC+AGG 0.541895 2.1:+62289059 None:intergenic
TGCCTTACAAGTTATGGAGG+AGG 0.551483 2.1:-62287305 MS.gene003221:CDS
TGTCAAGATGATATTTCTGA+AGG 0.552931 2.1:-62289101 MS.gene003221:CDS
AAACAACCATGAATACGATT+CGG 0.553116 2.1:+62288287 None:intergenic
ACGACATGCAGCTTTGGACC+TGG 0.553268 2.1:+62288446 None:intergenic
ATTACCCGATGGAGAATGCC+AGG 0.553334 2.1:-62289138 MS.gene003221:CDS
TTCCCCTCCGCATCAGAGCT+AGG 0.562167 2.1:+62288705 None:intergenic
CCTGACGCGCTGCATCGTAA+TGG 0.562464 2.1:+62287266 None:intergenic
TCAATGATGAAGGACATTTG+AGG 0.564984 2.1:-62288488 MS.gene003221:CDS
ATTTGCTATGCCAAAGAGGT+GGG 0.566055 2.1:-62289008 MS.gene003221:CDS
ATCTCTTTATAAGAAGTACA+AGG 0.566116 2.1:-62287812 MS.gene003221:CDS
ACCACTCAACTACGTGCAGA+TGG 0.567203 2.1:-62287640 MS.gene003221:CDS
AGTTAGTGGAGTCTCAGAGA+TGG 0.573080 2.1:-62288827 MS.gene003221:CDS
TAGTTAGCTGCATACGACAT+AGG 0.573239 2.1:+62288538 None:intergenic
AGGTTGAGACTCGCCGTCGA+CGG 0.574630 2.1:+62289481 None:intergenic
TATTTGCTATGCCAAAGAGG+TGG 0.575475 2.1:-62289009 MS.gene003221:CDS
ATTCCAACAAAAGTCACAAG+AGG 0.580554 2.1:+62288366 None:intergenic
CTATCCGTAATAATAGTTTG+AGG 0.582240 2.1:+62288231 None:intergenic
GACAATCTAATTCAATTACC+TGG 0.587039 2.1:+62289120 None:intergenic
TTGTCAAGAAAATCCTTCAA+AGG 0.587595 2.1:+62287844 None:intergenic
TAGTGGAGTCTCAGAGATGG+AGG 0.590281 2.1:-62288824 MS.gene003221:CDS
GCCTTACAAGTTATGGAGGA+GGG 0.592013 2.1:-62287304 MS.gene003221:CDS
CACCGTGATAGAATGTACTT+AGG 0.598150 2.1:+62287424 None:intergenic
CGTGATAGAATGTACTTAGG+AGG 0.599389 2.1:+62287427 None:intergenic
ATACTGGTGCCCAATGAACT+CGG 0.607374 2.1:+62287959 None:intergenic
ATTGAATTTGAGGCGGCATG+GGG 0.607914 2.1:-62288042 MS.gene003221:CDS
TTATATTTGCTATGCCAAAG+AGG 0.610702 2.1:-62289012 MS.gene003221:CDS
CTCGTTGCCTAGCTCTGATG+CGG 0.616476 2.1:-62288712 MS.gene003221:CDS
GCCATCTGCACGTAGTTGAG+TGG 0.627400 2.1:+62287639 None:intergenic
TGCCTAGCTCTGATGCGGAG+GGG 0.628559 2.1:-62288707 MS.gene003221:CDS
CTCCTAAGTACATTCTATCA+CGG 0.629356 2.1:-62287426 MS.gene003221:CDS
TTCATGATGTAAAACTTGAG+AGG 0.630957 2.1:-62287219 MS.gene003221:CDS
CAAAAGCTTGACACCGTCGA+CGG 0.630980 2.1:-62289494 MS.gene003221:CDS
TGAACTCGGTCTTCATATAG+GGG 0.631197 2.1:+62287973 None:intergenic
AAGTGCCTTACAAGTTATGG+AGG 0.637921 2.1:-62287308 MS.gene003221:CDS
GTATCTTAGCACCTAAGACG+TGG 0.639364 2.1:+62288775 None:intergenic
TTTGTTGGAATAAATCACCA+TGG 0.640231 2.1:-62288354 MS.gene003221:CDS
AGAAGAACAAGAACACCCAA+AGG 0.641728 2.1:-62289453 MS.gene003221:intron
CTCAAACTATTATTACGGAT+AGG 0.645642 2.1:-62288230 MS.gene003221:CDS
GAAAGTGATTGAATTTGAGG+CGG 0.650309 2.1:-62288049 MS.gene003221:CDS
TTGAAGTTAGGGATAACGAG+TGG 0.652213 2.1:-62288002 MS.gene003221:CDS
CTAATAACACAGATTGACCA+TGG 0.669427 2.1:+62288337 None:intergenic
GCACTTGTGATAGATACAGA+AGG 0.672329 2.1:-62288663 MS.gene003221:CDS
TAAAGCAAGAACAAATACAA+CGG 0.685448 2.1:+62287515 None:intergenic
TCAGAGATGGAGGATATGTG+AGG 0.701673 2.1:-62288814 MS.gene003221:CDS
TGTTGTGATCAATTACCCGA+TGG 0.706322 2.1:-62289149 MS.gene003221:CDS
CTAAGTACATTCTATCACGG+TGG 0.712046 2.1:-62287423 MS.gene003221:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATAAATTAGTTAAAAAGA+TGG - chr2.1:62287977-62287996 MS.gene003221:CDS 15.0%
!! ATGAAAAATTAATTACCTTT+GGG + chr2.1:62287296-62287315 None:intergenic 15.0%
!! TATGAAAAATTAATTACCTT+TGG + chr2.1:62287297-62287316 None:intergenic 15.0%
!! AACATGTATTTATCGAAAAA+AGG + chr2.1:62288857-62288876 None:intergenic 20.0%
!!! ATTTTTATGGATATCTATGT+CGG - chr2.1:62289239-62289258 MS.gene003221:intron 20.0%
!!! TGTTCTTGCTTTAATTTTTA+TGG - chr2.1:62289226-62289245 MS.gene003221:intron 20.0%
!!! TTTTGAAGAATCACTTAATA+AGG - chr2.1:62289489-62289508 MS.gene003221:CDS 20.0%
! ACATTTGAGGAATGTATTAT+GGG - chr2.1:62288256-62288275 MS.gene003221:CDS 25.0%
! ACGACATAGGAAATTATAAA+TGG + chr2.1:62288183-62288202 None:intergenic 25.0%
! ATGATATTGAACGTATTCAA+TGG - chr2.1:62289383-62289402 MS.gene003221:intron 25.0%
! ATTGAAAGTGATTGAATTTG+AGG - chr2.1:62288679-62288698 MS.gene003221:CDS 25.0%
! CGAAATAGCAAAGAAAAATA+TGG - chr2.1:62287780-62287799 MS.gene003221:CDS 25.0%
! CGTATCTAACTTATTTCAAA+TGG + chr2.1:62287390-62287409 None:intergenic 25.0%
! TAAAGCAAGAACAAATACAA+CGG + chr2.1:62289219-62289238 None:intergenic 25.0%
! TCCATCAAATAAAAGAAGTT+TGG + chr2.1:62288221-62288240 None:intergenic 25.0%
! TTCTCCTCAAACTATTATTA+CGG - chr2.1:62288496-62288515 MS.gene003221:CDS 25.0%
!! AATAAGTTGAACCTAAGAAA+AGG - chr2.1:62288146-62288165 MS.gene003221:CDS 25.0%
!! ATCTCTTTATAAGAAGTACA+AGG - chr2.1:62288919-62288938 MS.gene003221:CDS 25.0%
!!! AAAAAAGTACCAGAAAAACT+GGG - chr2.1:62288599-62288618 MS.gene003221:CDS 25.0%
!!! GATTCAACATTTTGAAGTTA+GGG - chr2.1:62288718-62288737 MS.gene003221:CDS 25.0%
!!! TCCAAACTTCTTTTATTTGA+TGG - chr2.1:62288217-62288236 MS.gene003221:CDS 25.0%
!!! TGATTCAACATTTTGAAGTT+AGG - chr2.1:62288717-62288736 MS.gene003221:CDS 25.0%
!!! TGTGTGTACTCAATTTTAAT+GGG - chr2.1:62289269-62289288 MS.gene003221:intron 25.0%
AAACAACCATGAATACGATT+CGG + chr2.1:62288447-62288466 None:intergenic 30.0%
AACTTGTCCTTTATGTATAC+TGG + chr2.1:62288791-62288810 None:intergenic 30.0%
AGTTGTGATCAGGAATATAA+AGG + chr2.1:62289351-62289370 None:intergenic 30.0%
CAATGGTCTAATCAGTTATT+TGG - chr2.1:62289400-62289419 MS.gene003221:intron 30.0%
CACTAACTTAATCCTTATCA+TGG + chr2.1:62287892-62287911 None:intergenic 30.0%
CATGATAAGGATTAAGTTAG+TGG - chr2.1:62287890-62287909 MS.gene003221:CDS 30.0%
CTATCCGTAATAATAGTTTG+AGG + chr2.1:62288503-62288522 None:intergenic 30.0%
CTCAAACTATTATTACGGAT+AGG - chr2.1:62288501-62288520 MS.gene003221:CDS 30.0%
GACAATCTAATTCAATTACC+TGG + chr2.1:62287614-62287633 None:intergenic 30.0%
GACATTTGAGGAATGTATTA+TGG - chr2.1:62288255-62288274 MS.gene003221:CDS 30.0%
TACATAAAGGACAAGTTTCT+TGG - chr2.1:62288794-62288813 MS.gene003221:CDS 30.0%
TTAGTTAAAAAGATGGCAAG+TGG - chr2.1:62287984-62288003 MS.gene003221:CDS 30.0%
TTATGAATTACCGTAGATCT+TGG + chr2.1:62287340-62287359 None:intergenic 30.0%
TTCATGATGTAAAACTTGAG+AGG - chr2.1:62289512-62289531 MS.gene003221:CDS 30.0%
TTGTCAAGAAAATCCTTCAA+AGG + chr2.1:62288890-62288909 None:intergenic 30.0%
TTTGACCAAGAAGATTATGA+TGG + chr2.1:62289119-62289138 None:intergenic 30.0%
TTTGTTGGAATAAATCACCA+TGG - chr2.1:62288377-62288396 MS.gene003221:CDS 30.0%
! AATCTGTGTTATTAGGTTGT+GGG - chr2.1:62288402-62288421 MS.gene003221:CDS 30.0%
! ATTCATGGTTGTTTAGAACA+TGG - chr2.1:62288453-62288472 MS.gene003221:CDS 30.0%
! CTGTGTGTACTCAATTTTAA+TGG - chr2.1:62289268-62289287 MS.gene003221:intron 30.0%
! GTCAATTTAGATTTTGCGTT+AGG - chr2.1:62287490-62287509 MS.gene003221:CDS 30.0%
! TGTCAAGATGATATTTCTGA+AGG - chr2.1:62287630-62287649 MS.gene003221:CDS 30.0%
! TTCATGGTTGTTTAGAACAT+GGG - chr2.1:62288454-62288473 MS.gene003221:CDS 30.0%
!! ATTTTGTTTGACGATATGCT+AGG - chr2.1:62287542-62287561 MS.gene003221:CDS 30.0%
!! GAAAAAAGTACCAGAAAAAC+TGG - chr2.1:62288598-62288617 MS.gene003221:CDS 30.0%
!! GAAGAAATGTTCTCTTGTTT+TGG - chr2.1:62289067-62289086 MS.gene003221:CDS 30.0%
!! GTGTGTACTCAATTTTAATG+GGG - chr2.1:62289270-62289289 MS.gene003221:intron 30.0%
!! TTATATTTGCTATGCCAAAG+AGG - chr2.1:62287719-62287738 MS.gene003221:CDS 30.0%
!! TTGATGGATTTCAATGATGA+AGG - chr2.1:62288233-62288252 MS.gene003221:CDS 30.0%
AAAATAACGACATGCAGCTT+TGG + chr2.1:62288294-62288313 None:intergenic 35.0%
AACTTAATCCTTATCATGGC+AGG + chr2.1:62287888-62287907 None:intergenic 35.0%
AATGAACTCGGTCTTCATAT+AGG + chr2.1:62288763-62288782 None:intergenic 35.0%
ACATTTCTTCCACTTCAAGT+TGG + chr2.1:62289057-62289076 None:intergenic 35.0%
ATAAAGGACAAGTTTCTTGG+TGG - chr2.1:62288797-62288816 MS.gene003221:CDS 35.0%
ATATGAAGACCGAGTTCATT+GGG - chr2.1:62288763-62288782 MS.gene003221:CDS 35.0%
ATGAACTCGGTCTTCATATA+GGG + chr2.1:62288762-62288781 None:intergenic 35.0%
ATTCCAACAAAAGTCACAAG+AGG + chr2.1:62288368-62288387 None:intergenic 35.0%
CCAAAACAATGATGAGATCT+GGG + chr2.1:62288563-62288582 None:intergenic 35.0%
CTAATAACACAGATTGACCA+TGG + chr2.1:62288397-62288416 None:intergenic 35.0%
CTCCTAAGTACATTCTATCA+CGG - chr2.1:62289305-62289324 MS.gene003221:intron 35.0%
GAAAGTGATTGAATTTGAGG+CGG - chr2.1:62288682-62288701 MS.gene003221:CDS 35.0%
GTGATAGATACAGAAGGTAA+TGG - chr2.1:62288074-62288093 MS.gene003221:CDS 35.0%
TATATGAAGACCGAGTTCAT+TGG - chr2.1:62288762-62288781 MS.gene003221:CDS 35.0%
TATATTCCTGATCACAACTC+TGG - chr2.1:62289352-62289371 MS.gene003221:intron 35.0%
TCAATGATGAAGGACATTTG+AGG - chr2.1:62288243-62288262 MS.gene003221:CDS 35.0%
TGTTCTTGTTCTTCTTCTTG+AGG + chr2.1:62287273-62287292 None:intergenic 35.0%
TTGAGAAAAGAAACCAGAAC+TGG - chr2.1:62287858-62287877 MS.gene003221:CDS 35.0%
! ATTCCTCTTGTGACTTTTGT+TGG - chr2.1:62288362-62288381 MS.gene003221:CDS 35.0%
! CAATCTGTGTTATTAGGTTG+TGG - chr2.1:62288401-62288420 MS.gene003221:CDS 35.0%
! CATGGTCAATCTGTGTTATT+AGG - chr2.1:62288395-62288414 MS.gene003221:CDS 35.0%
!! TGTACTCAATTTTAATGGGG+TGG - chr2.1:62289273-62289292 MS.gene003221:intron 35.0%
!!! AAAGAGCGTGTTTTGATTGA+GGG - chr2.1:62289136-62289155 MS.gene003221:CDS 35.0%
AAACGACCGAATCGTATTCA+TGG - chr2.1:62288438-62288457 MS.gene003221:CDS 40.0%
AAGTACCAGAAAAACTGGGA+GGG - chr2.1:62288603-62288622 MS.gene003221:CDS 40.0%
AAGTGCCTTACAAGTTATGG+AGG - chr2.1:62289423-62289442 MS.gene003221:intron 40.0%
AATTACCTGGCATTCTCCAT+CGG + chr2.1:62287601-62287620 None:intergenic 40.0%
AGAAGAACAAGAACACCCAA+AGG - chr2.1:62287278-62287297 MS.gene003221:CDS 40.0%
CACCGTGATAGAATGTACTT+AGG + chr2.1:62289310-62289329 None:intergenic 40.0%
CATGAAGTTCCAACTTGAAG+TGG - chr2.1:62289045-62289064 MS.gene003221:CDS 40.0%
CGTGATAGAATGTACTTAGG+AGG + chr2.1:62289307-62289326 None:intergenic 40.0%
CTAAGTACATTCTATCACGG+TGG - chr2.1:62289308-62289327 MS.gene003221:intron 40.0%
GACATTAAATTTCGCTACCC+AGG + chr2.1:62287473-62287492 None:intergenic 40.0%
GCACTTGTGATAGATACAGA+AGG - chr2.1:62288068-62288087 MS.gene003221:CDS 40.0%
GCCAAAACAATGATGAGATC+TGG + chr2.1:62288564-62288583 None:intergenic 40.0%
GTTCAGACATACACCTTTGA+AGG - chr2.1:62288874-62288893 MS.gene003221:CDS 40.0%
TAGGCACGCTGATATTGTTT+AGG - chr2.1:62287509-62287528 MS.gene003221:CDS 40.0%
TAGTTAGCTGCATACGACAT+AGG + chr2.1:62288196-62288215 None:intergenic 40.0%
TCAAATTCCATTCCAAGAGC+AGG + chr2.1:62287675-62287694 None:intergenic 40.0%
TGAACTCGGTCTTCATATAG+GGG + chr2.1:62288761-62288780 None:intergenic 40.0%
TGGCTCCATCATAATCTTCT+TGG - chr2.1:62289111-62289130 MS.gene003221:CDS 40.0%
TGTTGTGATCAATTACCCGA+TGG - chr2.1:62287582-62287601 MS.gene003221:CDS 40.0%
TTACCGTAGATCTTGGAAAG+AGG + chr2.1:62287333-62287352 None:intergenic 40.0%
TTCTGCAATTGCACTTTGCA+AGG + chr2.1:62288534-62288553 None:intergenic 40.0%
TTGAAGTTAGGGATAACGAG+TGG - chr2.1:62288729-62288748 MS.gene003221:CDS 40.0%
TTGCCTCTTTCCAAGATCTA+CGG - chr2.1:62287327-62287346 MS.gene003221:CDS 40.0%
TTGGGCACCAGTATACATAA+AGG - chr2.1:62288781-62288800 MS.gene003221:CDS 40.0%
TTGTTTGACGATATGCTAGG+TGG - chr2.1:62287545-62287564 MS.gene003221:CDS 40.0%
TTTGATTGAGGGAAACAGAC+AGG - chr2.1:62289147-62289166 MS.gene003221:CDS 40.0%
! AAAGTACCAGAAAAACTGGG+AGG - chr2.1:62288602-62288621 MS.gene003221:CDS 40.0%
! ACATGGGTTACTTGTATGTC+AGG - chr2.1:62288470-62288489 MS.gene003221:CDS 40.0%
! CCCAGATCTCATCATTGTTT+TGG - chr2.1:62288560-62288579 MS.gene003221:CDS 40.0%
! CTTGTGAGTCACCTTTTCTT+AGG + chr2.1:62288160-62288179 None:intergenic 40.0%
!! ATGTATTATGGGTTGATGCC+AGG - chr2.1:62288267-62288286 MS.gene003221:CDS 40.0%
!! ATTTGCTATGCCAAAGAGGT+GGG - chr2.1:62287723-62287742 MS.gene003221:CDS 40.0%
!! CTGCAGTTCAAAAGAGCATT+TGG + chr2.1:62288996-62289015 None:intergenic 40.0%
!! TATTTGCTATGCCAAAGAGG+TGG - chr2.1:62287722-62287741 MS.gene003221:CDS 40.0%
!! TGGAAGTGCCTTACAAGTTA+TGG - chr2.1:62289420-62289439 MS.gene003221:intron 40.0%
!!! AGAGCATTTGGTTTTCAGCA+AGG + chr2.1:62288984-62289003 None:intergenic 40.0%
!!! AGCATTTGGTTTTCAGCAAG+GGG + chr2.1:62288982-62289001 None:intergenic 40.0%
!!! CAAAGAGCGTGTTTTGATTG+AGG - chr2.1:62289135-62289154 MS.gene003221:CDS 40.0%
!!! GAGCATTTGGTTTTCAGCAA+GGG + chr2.1:62288983-62289002 None:intergenic 40.0%
ACCTCTTGTGTAGGAAACCT+GGG - chr2.1:62287453-62287472 MS.gene003221:CDS 45.0%
AGCTCTGTCATCTGTTGCAT+TGG + chr2.1:62288111-62288130 None:intergenic 45.0%
AGTTAGTGGAGTCTCAGAGA+TGG - chr2.1:62287904-62287923 MS.gene003221:CDS 45.0%
ATACTGGTGCCCAATGAACT+CGG + chr2.1:62288775-62288794 None:intergenic 45.0%
ATGCTGCATACCTCTTGTGT+AGG - chr2.1:62287444-62287463 MS.gene003221:CDS 45.0%
ATTACCTGGCATTCTCCATC+GGG + chr2.1:62287600-62287619 None:intergenic 45.0%
CACACAAAATCCCACCTCTT+TGG + chr2.1:62287736-62287755 None:intergenic 45.0%
CTCGAACATAACCACGTCTT+AGG - chr2.1:62287945-62287964 MS.gene003221:CDS 45.0%
GAACTACCAGAGTTGTGATC+AGG + chr2.1:62289361-62289380 None:intergenic 45.0%
GCCTTACAAGTTATGGAGGA+GGG - chr2.1:62289427-62289446 MS.gene003221:intron 45.0%
GGACAAGTTTCTTGGTGGAA+TGG - chr2.1:62288802-62288821 MS.gene003221:CDS 45.0%
GGGAAACAGACAGGAGATAA+AGG - chr2.1:62289156-62289175 MS.gene003221:CDS 45.0%
GTATCTTAGCACCTAAGACG+TGG + chr2.1:62287959-62287978 None:intergenic 45.0%
TACCTCTTGTGTAGGAAACC+TGG - chr2.1:62287452-62287471 MS.gene003221:CDS 45.0%
TCAGAGATGGAGGATATGTG+AGG - chr2.1:62287917-62287936 MS.gene003221:CDS 45.0%
TGCCTTACAAGTTATGGAGG+AGG - chr2.1:62289426-62289445 MS.gene003221:intron 45.0%
! AAGAGGTGGGATTTTGTGTG+AGG - chr2.1:62287736-62287755 MS.gene003221:CDS 45.0%
! ATTGAATTTGAGGCGGCATG+GGG - chr2.1:62288689-62288708 MS.gene003221:CDS 45.0%
! GATTGAATTTGAGGCGGCAT+GGG - chr2.1:62288688-62288707 MS.gene003221:CDS 45.0%
! GTGCTTTGTTGTAGCAGTCA+AGG - chr2.1:62287807-62287826 MS.gene003221:CDS 45.0%
! TCCCTCCTCCATAACTTGTA+AGG + chr2.1:62289431-62289450 None:intergenic 45.0%
! TGATTGAATTTGAGGCGGCA+TGG - chr2.1:62288687-62288706 MS.gene003221:CDS 45.0%
ACCACTCAACTACGTGCAGA+TGG - chr2.1:62289091-62289110 MS.gene003221:CDS 50.0%
ACCCAGGTTTCCTACACAAG+AGG + chr2.1:62287457-62287476 None:intergenic 50.0%
ATTACCCGATGGAGAATGCC+AGG - chr2.1:62287593-62287612 MS.gene003221:CDS 50.0%
CAAAAGCTTGACACCGTCGA+CGG - chr2.1:62287237-62287256 MS.gene003221:CDS 50.0%
CTGGTTGTCCTGCCATGATA+AGG - chr2.1:62287877-62287896 MS.gene003221:CDS 50.0%
TAGTGGAGTCTCAGAGATGG+AGG - chr2.1:62287907-62287926 MS.gene003221:CDS 50.0%
TTCCATTCCAAGAGCAGGTG+CGG + chr2.1:62287670-62287689 None:intergenic 50.0%
! TTAGGTTGTGGGTTGCTTGC+AGG - chr2.1:62288413-62288432 MS.gene003221:CDS 50.0%
!! GTCGACGGTGTCAAGCTTTT+GGG + chr2.1:62287238-62287257 None:intergenic 50.0%
CATGGCAGGACAACCAGTTC+TGG + chr2.1:62287874-62287893 None:intergenic 55.0%
GAATTTCCCGCACCTGCTCT+TGG - chr2.1:62287660-62287679 MS.gene003221:CDS 55.0%
GCCATCTGCACGTAGTTGAG+TGG + chr2.1:62289095-62289114 None:intergenic 55.0%
TCCATTCCAAGAGCAGGTGC+GGG + chr2.1:62287669-62287688 None:intergenic 55.0%
! CGCAACCCTCCCAGTTTTTC+TGG + chr2.1:62288611-62288630 None:intergenic 55.0%
!! ACGACATGCAGCTTTGGACC+TGG + chr2.1:62288288-62288307 None:intergenic 55.0%
!! CGTCGACGGTGTCAAGCTTT+TGG + chr2.1:62287239-62287258 None:intergenic 55.0%
!! CTCGTTGCCTAGCTCTGATG+CGG - chr2.1:62288019-62288038 MS.gene003221:CDS 55.0%
!! TTGCCTAGCTCTGATGCGGA+GGG - chr2.1:62288023-62288042 MS.gene003221:CDS 55.0%
AGGTTGAGACTCGCCGTCGA+CGG + chr2.1:62287253-62287272 None:intergenic 60.0%
CCATTACGATGCAGCGCGTC+AGG - chr2.1:62289465-62289484 MS.gene003221:CDS 60.0%
CCTGACGCGCTGCATCGTAA+TGG + chr2.1:62289468-62289487 None:intergenic 60.0%
TCCCGCACCTGCTCTTGGAA+TGG - chr2.1:62287665-62287684 MS.gene003221:CDS 60.0%
TTCCCCTCCGCATCAGAGCT+AGG + chr2.1:62288029-62288048 None:intergenic 60.0%
!! GTTGCCTAGCTCTGATGCGG+AGG - chr2.1:62288022-62288041 MS.gene003221:CDS 60.0%
!! TGCCTAGCTCTGATGCGGAG+GGG - chr2.1:62288024-62288043 MS.gene003221:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 62287216 62289537 62287216 ID=MS.gene003221
chr2.1 mRNA 62287216 62289537 62287216 ID=MS.gene003221.t1;Parent=MS.gene003221
chr2.1 exon 62289454 62289537 62289454 ID=MS.gene003221.t1.exon1;Parent=MS.gene003221.t1
chr2.1 CDS 62289454 62289537 62289454 ID=cds.MS.gene003221.t1;Parent=MS.gene003221.t1
chr2.1 exon 62287216 62289189 62287216 ID=MS.gene003221.t1.exon2;Parent=MS.gene003221.t1
chr2.1 CDS 62287216 62289189 62287216 ID=cds.MS.gene003221.t1;Parent=MS.gene003221.t1
Gene Sequence

>MS.gene003221

ATGATGTCAGAGTTGCAGACCCAAAAGCTTGACACCGTCGACGGCGAGTCTCAACCTCAAGAAGAAGAACAAGAACACCCAAAGGTAATTAATTTTTCATATATATGCTCTTTGCCTCTTTCCAAGATCTACGGTAATTCATAATGAAACAAAATCTCAATTTAATTGTAGCCATTTGAAATAAGTTAGATACGTGATATATTTATGCAAGTAAGTGATTACATGTTTATGCTGCATACCTCTTGTGTAGGAAACCTGGGTAGCGAAATTTAATGTCAATTTAGATTTTGCGTTAGGCACGCTGATATTGTTTAGGTAGCTTATTGATTTTGTTTGACGATATGCTAGGTGGACGACATTTCTGTGTGTTGTGATCAATTACCCGATGGAGAATGCCAGGTAATTGAATTAGATTGTCAAGATGATATTTCTGAAGGAAGAAAAGAATTTCCCGCACCTGCTCTTGGAATGGAATTTGAGTCATATGATGATGCTTACAGTTATTATATTTGCTATGCCAAAGAGGTGGGATTTTGTGTGAGGATTAAGAATTCATGTTTCAAGCGAAATAGCAAAGAAAAATATGGTGCAGTGCTTTGTTGTAGCAGTCAAGGTTTCAATAGAACAAAAGATGTTGACATTTTGAGAAAAGAAACCAGAACTGGTTGTCCTGCCATGATAAGGATTAAGTTAGTGGAGTCTCAGAGATGGAGGATATGTGAGGTTACTCTCGAACATAACCACGTCTTAGGTGCTAAGATACATAAATTAGTTAAAAAGATGGCAAGTGGAACTAAAAAGAACTCGTTGCCTAGCTCTGATGCGGAGGGGAAAACAATTAAGATATATCATGCACTTGTGATAGATACAGAAGGTAATGGCAATTTAAACTCCAATGCAACAGATGACAGAGCTTTCTCGAAATATTCTAATAAGTTGAACCTAAGAAAAGGTGACTCACAAGCCATTTATAATTTCCTATGTCGTATGCAGCTAACTAATCCAAACTTCTTTTATTTGATGGATTTCAATGATGAAGGACATTTGAGGAATGTATTATGGGTTGATGCCAGGTCCAAAGCTGCATGTCGTTATTTTAGCGATGTCATTTATTTTGACAACACTTATTTGTCAAATAAATATGAAATTCCTCTTGTGACTTTTGTTGGAATAAATCACCATGGTCAATCTGTGTTATTAGGTTGTGGGTTGCTTGCAGGTGAAACGACCGAATCGTATTCATGGTTGTTTAGAACATGGGTTACTTGTATGTCAGGATGTTCTCCTCAAACTATTATTACGGATAGGTGCAAGACCTTGCAAAGTGCAATTGCAGAAAATTTTCCCAGATCTCATCATTGTTTTGGCTTGTCATTAGTTATGAAAAAAGTACCAGAAAAACTGGGAGGGTTGCGCAATTACGATGCAATCAGAAAAGCACTGATTAAAGCAGTTTATGAGACATTGAAAGTGATTGAATTTGAGGCGGCATGGGGATTTATGATTCAACATTTTGAAGTTAGGGATAACGAGTGGCTGCGTCCCCTATATGAAGACCGAGTTCATTGGGCACCAGTATACATAAAGGACAAGTTTCTTGGTGGAATGGCTACTACACGCTGTAGTGAGAGCATTAGTCCTTTTTTCGATAAATACATGTTCAGACATACACCTTTGAAGGATTTTCTTGACAAGTATGAATTATCTCTTTATAAGAAGTACAAGGAAGAATCTTTAGCAGATATTGAATCTAGAAGCTCTAGCCCCTTGCTGAAAACCAAATGCTCTTTTGAACTGCAGTTGTCTAGCATGTACACAAAAGAAATATTCATGAAGTTCCAACTTGAAGTGGAAGAAATGTTCTCTTGTTTTGGTACCACTCAACTACGTGCAGATGGCTCCATCATAATCTTCTTGGTCAAAGAGCGTGTTTTGATTGAGGGAAACAGACAGGAGATAAAGGATTTTGAAGTTGTTTACAGTAAATCAGCATGTGAAGTCCGTTGTATTTGTTCTTGCTTTAATTTTTATGGATATCTATGTCGGCATGCGCTGTGTGTACTCAATTTTAATGGGGTGGAAGAGATTCCTCCTAAGTACATTCTATCACGGTGGAAGAAAGATTACAAACGCCTTTATATTCCTGATCACAACTCTGGTAGTTCTGATGATATTGAACGTATTCAATGGTCTAATCAGTTATTTGGAAGTGCCTTACAAGTTATGGAGGAGGGAATGATTTCTATAGACCATTACGATGCAGCGCGTCAGGCTTTTGAAGAATCACTTAATAAGGTTCATGATGTAAAACTTGAGAGGTGA
Protein sequence

>MS.gene003221.t1

MMSELQTQKLDTVDGESQPQEEEQEHPKVDDISVCCDQLPDGECQVIELDCQDDISEGRKEFPAPALGMEFESYDDAYSYYICYAKEVGFCVRIKNSCFKRNSKEKYGAVLCCSSQGFNRTKDVDILRKETRTGCPAMIRIKLVESQRWRICEVTLEHNHVLGAKIHKLVKKMASGTKKNSLPSSDAEGKTIKIYHALVIDTEGNGNLNSNATDDRAFSKYSNKLNLRKGDSQAIYNFLCRMQLTNPNFFYLMDFNDEGHLRNVLWVDARSKAACRYFSDVIYFDNTYLSNKYEIPLVTFVGINHHGQSVLLGCGLLAGETTESYSWLFRTWVTCMSGCSPQTIITDRCKTLQSAIAENFPRSHHCFGLSLVMKKVPEKLGGLRNYDAIRKALIKAVYETLKVIEFEAAWGFMIQHFEVRDNEWLRPLYEDRVHWAPVYIKDKFLGGMATTRCSESISPFFDKYMFRHTPLKDFLDKYELSLYKKYKEESLADIESRSSSPLLKTKCSFELQLSSMYTKEIFMKFQLEVEEMFSCFGTTQLRADGSIIIFLVKERVLIEGNRQEIKDFEVVYSKSACEVRCICSCFNFYGYLCRHALCVLNFNGVEEIPPKYILSRWKKDYKRLYIPDHNSGSSDDIERIQWSNQLFGSALQVMEEGMISIDHYDAARQAFEESLNKVHDVKLER