AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00323

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00323.t1 MTR_2g437060 89.367 395 40 1 1 393 1 395 0.0 714
MS.gene00323.t1 MTR_2g436400 76.093 389 83 3 1 387 1 381 0.0 583
MS.gene00323.t1 MTR_2g437080 76.042 384 82 2 9 392 6 379 0.0 583
MS.gene00323.t1 MTR_2g437020 67.089 395 113 4 1 393 1 380 0.0 521
MS.gene00323.t1 MTR_2g436440 65.903 393 125 4 1 392 1 385 3.87e-180 506
MS.gene00323.t1 MTR_2g437030 66.580 386 125 2 9 393 84 466 1.21e-177 503
MS.gene00323.t1 MTR_2g437040 64.888 356 125 0 38 393 25 380 7.08e-165 467
MS.gene00323.t1 MTR_2g437160 51.771 367 170 3 31 391 38 403 1.88e-125 368
MS.gene00323.t1 MTR_2g437170 50.909 385 178 6 18 391 14 398 3.64e-125 367
MS.gene00323.t1 MTR_4g119550 52.174 345 162 2 49 392 47 389 9.75e-125 365
MS.gene00323.t1 MTR_2g437120 53.005 366 166 4 31 391 27 391 1.09e-124 366
MS.gene00323.t1 MTR_2g437200 50.131 383 149 4 41 383 18 398 3.91e-123 362
MS.gene00323.t1 MTR_2g437150 50.524 382 178 6 18 388 14 395 1.10e-122 361
MS.gene00323.t1 MTR_2g437960 51.462 342 163 2 55 393 55 396 1.05e-119 353
MS.gene00323.t1 MTR_2g437960 51.462 342 163 2 55 393 55 396 2.07e-119 353
MS.gene00323.t1 MTR_2g437260 49.288 351 172 4 41 387 22 370 8.95e-119 350
MS.gene00323.t1 MTR_2g437990 49.725 364 174 3 36 391 28 390 4.57e-118 350
MS.gene00323.t1 MTR_2g437180 48.429 382 189 5 14 391 11 388 3.73e-117 346
MS.gene00323.t1 MTR_2g438010 50.552 362 167 5 36 387 48 407 6.42e-117 347
MS.gene00323.t1 MTR_2g076320 49.454 366 165 4 36 387 28 387 1.13e-116 345
MS.gene00323.t1 MTR_2g437130 49.036 363 181 2 29 391 21 379 1.87e-115 342
MS.gene00323.t1 MTR_2g437100 51.630 368 163 6 31 383 28 395 1.23e-114 340
MS.gene00323.t1 MTR_2g437240 46.154 403 171 5 27 391 12 406 3.28e-113 337
MS.gene00323.t1 MTR_2g437940 49.041 365 153 6 31 391 27 362 7.99e-109 324
MS.gene00323.t1 MTR_2g438020 46.561 378 162 3 52 391 70 445 3.72e-108 327
MS.gene00323.t1 MTR_2g436460 46.512 344 181 2 44 386 38 379 5.08e-104 312
MS.gene00323.t1 MTR_4g120380 42.049 371 206 3 11 381 9 370 7.54e-91 278
MS.gene00323.t1 MTR_2g436370 70.588 204 49 4 1 202 1 195 4.21e-90 270
MS.gene00323.t1 MTR_4g119580 42.522 341 192 2 43 381 32 370 2.62e-89 275
MS.gene00323.t1 MTR_2g019840 46.084 332 173 4 54 383 44 371 3.13e-88 272
MS.gene00323.t1 MTR_4g120380 43.020 351 191 3 11 361 9 350 4.18e-86 268
MS.gene00323.t1 MTR_2g019810 44.277 332 179 4 54 383 32 359 3.17e-85 264
MS.gene00323.t1 MTR_2g437090 64.881 168 59 0 226 393 1 168 1.88e-76 234
MS.gene00323.t1 MTR_2g437160 55.721 201 86 1 194 391 112 312 1.56e-71 227
MS.gene00323.t1 MTR_3g450500 32.635 334 217 3 54 381 39 370 4.71e-52 178
MS.gene00323.t1 MTR_4g007490 32.303 356 231 7 33 381 16 368 2.60e-45 160
MS.gene00323.t1 MTR_4g119570 35.743 249 127 1 133 381 14 229 3.16e-45 156
MS.gene00323.t1 MTR_8g080990 29.831 295 204 2 86 377 74 368 2.15e-33 128
MS.gene00323.t1 MTR_8g081000 26.409 337 244 3 48 381 50 385 3.59e-28 114
MS.gene00323.t1 MTR_8g081000 26.409 337 244 3 48 381 50 385 5.14e-28 114
MS.gene00323.t1 MTR_8g081000 26.409 337 244 3 48 381 50 385 8.01e-28 114
MS.gene00323.t1 MTR_2g436380 53.535 99 43 2 226 324 1 96 5.87e-24 100
MS.gene00323.t1 MTR_4g049400 43.307 127 67 2 33 154 25 151 1.63e-23 98.2
MS.gene00323.t1 MTR_3g085240 36.913 149 84 3 27 175 32 170 1.01e-18 85.5
MS.gene00323.t1 MTR_3g085240 46.067 89 31 1 285 373 176 247 9.68e-17 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00323.t1 AT5G07900 36.637 333 203 4 54 380 69 399 7.29e-69 223
MS.gene00323.t1 AT1G21150 29.912 341 231 4 44 378 10 348 4.07e-48 167
MS.gene00323.t1 AT1G21150 30.816 331 221 4 54 378 56 384 5.17e-48 168
MS.gene00323.t1 AT1G21150 30.816 331 221 4 54 378 120 448 1.59e-47 168
MS.gene00323.t1 AT1G61980 28.455 369 219 8 54 381 52 416 4.50e-34 131
MS.gene00323.t1 AT1G61980 28.455 369 219 8 54 381 52 416 4.50e-34 131
MS.gene00323.t1 AT1G61980 28.455 369 219 8 54 381 52 416 4.50e-34 131
MS.gene00323.t1 AT1G62120 28.134 359 205 9 55 368 57 407 6.98e-32 125
MS.gene00323.t1 AT1G61970 26.685 356 208 7 55 366 53 399 1.07e-31 124
MS.gene00323.t1 AT1G61970 26.685 356 208 7 55 366 53 399 1.07e-31 124
MS.gene00323.t1 AT1G61970 26.685 356 208 7 55 366 53 399 1.07e-31 124
MS.gene00323.t1 AT1G61970 26.685 356 208 7 55 366 53 399 1.07e-31 124
MS.gene00323.t1 AT1G61970 26.685 356 208 7 55 366 53 399 1.07e-31 124
MS.gene00323.t1 AT1G61970 26.685 356 208 7 55 366 53 399 1.07e-31 124
MS.gene00323.t1 AT1G61970 26.685 356 208 7 55 366 53 399 1.07e-31 124
MS.gene00323.t1 AT5G64950 28.691 359 233 8 44 388 26 375 7.64e-31 122
MS.gene00323.t1 AT1G61990 27.654 358 201 8 55 368 53 396 1.66e-30 121
MS.gene00323.t1 AT1G61990 27.654 358 201 8 55 368 53 396 1.66e-30 121
MS.gene00323.t1 AT1G62085 27.543 403 196 12 27 342 21 414 5.46e-29 117
MS.gene00323.t1 AT1G62085 27.543 403 196 12 27 342 21 414 5.46e-29 117
MS.gene00323.t1 AT3G46950 31.086 267 173 5 37 298 31 291 5.16e-28 115
MS.gene00323.t1 AT5G23930 27.110 391 203 10 54 371 55 436 4.60e-26 109
MS.gene00323.t1 AT1G62110 27.211 294 194 5 8 296 17 295 1.12e-22 99.8
MS.gene00323.t1 AT1G62010 24.232 293 184 3 87 343 69 359 1.95e-22 98.6
MS.gene00323.t1 AT1G61960 29.600 250 160 4 55 296 53 294 3.09e-22 98.6
MS.gene00323.t1 AT1G62150 23.733 375 199 9 48 343 52 418 3.94e-20 92.4

Find 86 sgRNAs with CRISPR-Local

Find 107 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATACCTCATTCACAATTT+TGG 0.061494 2.1:-56262842 MS.gene00323:CDS
GCAATCTGACAAGGAAATTT+TGG 0.125855 2.1:-56262501 MS.gene00323:CDS
CGACCAACCCCATTTCTTAA+AGG 0.171385 2.1:+56262217 None:intergenic
GGAAAAGAGGGTTAGGGTTT+AGG 0.191466 2.1:+56262933 None:intergenic
GTGAAGGAATTAAAGGATTT+GGG 0.210460 2.1:-56262326 MS.gene00323:CDS
GAAAAGAGGGTTAGGGTTTA+GGG 0.220235 2.1:+56262934 None:intergenic
GGTGAAGGAATTAAAGGATT+TGG 0.236355 2.1:-56262327 MS.gene00323:CDS
TTAATTTAGTGATGAGCTTT+TGG 0.242008 2.1:-56262130 MS.gene00323:CDS
AATCATTCTAAAACAACTTT+TGG 0.247310 2.1:-56262299 MS.gene00323:CDS
TGACAAAATATCAGGTGTTT+TGG 0.255478 2.1:+56262073 None:intergenic
GTGCAATTTCTTCTCAATAA+TGG 0.259750 2.1:-56262011 MS.gene00323:CDS
TAATCCCTTGAGATAGATTA+AGG 0.266032 2.1:+56262979 None:intergenic
CAAGTTGGTGAAGGAATTAA+AGG 0.289341 2.1:-56262333 MS.gene00323:CDS
TTTCTCATTCCACAAGGTTT+TGG 0.304420 2.1:+56262247 None:intergenic
TTATCTTATAAAGTTGTATC+AGG 0.309342 2.1:-56261892 MS.gene00323:CDS
TGAGAACTGAATCAGGGTTT+TGG 0.309396 2.1:+56262759 None:intergenic
AAACTTTGGCAACACCCTTT+TGG 0.315392 2.1:+56262652 None:intergenic
CTTGTAACTGGAGCAAAGTT+TGG 0.321987 2.1:+56262799 None:intergenic
AAGAAATTGAGAACTGAATC+AGG 0.327189 2.1:+56262752 None:intergenic
CTTGAAACGGAGCTTGTAAC+TGG 0.364053 2.1:+56262787 None:intergenic
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.1:-56262111 MS.gene00323:CDS
AAGTTGATGCCTTTAAGAAA+TGG 0.366199 2.1:-56262226 MS.gene00323:CDS
AGTTGATGCCTTTAAGAAAT+GGG 0.371481 2.1:-56262225 MS.gene00323:CDS
AAACTTAATGACAAAATATC+AGG 0.378240 2.1:+56262065 None:intergenic
AAATGGAGAGAAAAGAGAAA+TGG 0.389921 2.1:+56262902 None:intergenic
CAGAAGCGTTGGTGCAGAAA+TGG 0.394609 2.1:+56262885 None:intergenic
GCTTAATTTATCCATTCTTA+AGG 0.397953 2.1:-56261968 MS.gene00323:CDS
CAAACATTAAAAGATTGCTT+AGG 0.402903 2.1:-56262391 MS.gene00323:CDS
GTCAGAAGTAGAAGCGCCTT+TGG 0.405353 2.1:+56262616 None:intergenic
AATGGAGAGAAAAGAGAAAT+GGG 0.410398 2.1:+56262903 None:intergenic
AAAAGAGAAATGGGTAATGT+TGG 0.413194 2.1:+56262912 None:intergenic
AATAGTCCCAACCTATGAAT+TGG 0.421560 2.1:-56262537 MS.gene00323:CDS
TAATGTTGGAAAAGAGGGTT+AGG 0.425634 2.1:+56262926 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.429067 2.1:-56262112 MS.gene00323:CDS
GGAAGTAGCCATGGTGCCTT+GGG 0.435586 2.1:+56262686 None:intergenic
GGGAAGTAGCCATGGTGCCT+TGG 0.446977 2.1:+56262685 None:intergenic
AAACATTAAAAGATTGCTTA+GGG 0.465201 2.1:-56262390 MS.gene00323:CDS
TTCCCTGCGACCCCTCCAAA+AGG 0.466368 2.1:-56262667 MS.gene00323:CDS
CTCAATTTCTTTAGAAACCA+TGG 0.466986 2.1:-56262740 MS.gene00323:CDS
GGGAATGATACGACGTAGTT+GGG 0.467688 2.1:+56262706 None:intergenic
AGGCTTACAGACAAGACGCC+AGG 0.474816 2.1:+56262569 None:intergenic
GAATATGTACACCAATTCAT+AGG 0.478001 2.1:+56262526 None:intergenic
ATCACAATGCTGATTGAGAA+TGG 0.486666 2.1:-56262425 MS.gene00323:CDS
TGGGAATGATACGACGTAGT+TGG 0.489187 2.1:+56262705 None:intergenic
CAAAGTTTGGAAGCAGATTG+TGG 0.492532 2.1:+56262812 None:intergenic
GATTGCTTAGGGAACATACT+CGG 0.497604 2.1:-56262379 MS.gene00323:CDS
ATGGGTAATGTTGGAAAAGA+GGG 0.501669 2.1:+56262921 None:intergenic
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2.1:-56262102 MS.gene00323:CDS
TTGGGACTATTTGATTCTTG+AGG 0.509547 2.1:+56262549 None:intergenic
TGTACACCAATTCATAGGTT+GGG 0.509655 2.1:+56262531 None:intergenic
AAAGTTTGGAAGCAGATTGT+GGG 0.511078 2.1:+56262813 None:intergenic
AATGGGTAATGTTGGAAAAG+AGG 0.520798 2.1:+56262920 None:intergenic
AACTTCTCAGACCTTAAGAA+TGG 0.523239 2.1:+56261957 None:intergenic
GGGTCGCAGGGAAGTAGCCA+TGG 0.532044 2.1:+56262677 None:intergenic
AACTCAAGCTTGCATACACC+AGG 0.537467 2.1:-56261866 MS.gene00323:CDS
GAGGTATGAGACAGCAAAAG+AGG 0.548116 2.1:+56262856 None:intergenic
AGAGGTTGAATCAGAAGCGT+TGG 0.556682 2.1:+56262874 None:intergenic
CGCCAGGGTTCTTACACACA+AGG 0.557000 2.1:+56262585 None:intergenic
ATGTACACCAATTCATAGGT+TGG 0.561799 2.1:+56262530 None:intergenic
TCCCTGCGACCCCTCCAAAA+GGG 0.564822 2.1:-56262666 MS.gene00323:CDS
ATTGCTTAGGGAACATACTC+GGG 0.568007 2.1:-56262378 MS.gene00323:CDS
CGAGCCCAAAATTGTGAATG+AGG 0.569173 2.1:+56262837 None:intergenic
GTTGATGCCTTTAAGAAATG+GGG 0.573670 2.1:-56262224 MS.gene00323:CDS
TGTTCAAGTTCAACAATACA+TGG 0.576396 2.1:-56263012 MS.gene00323:CDS
CAGAACCTTAATCTATCTCA+AGG 0.578893 2.1:-56262984 MS.gene00323:CDS
TGAAGGAATTAAAGGATTTG+GGG 0.584499 2.1:-56262325 MS.gene00323:CDS
TGACAATCATGTCTTGTCCC+TGG 0.585106 2.1:+56261848 None:intergenic
GACACGAGACATGCTCAAGT+TGG 0.588113 2.1:-56262348 MS.gene00323:CDS
AGAAATTGAGAACTGAATCA+GGG 0.588690 2.1:+56262753 None:intergenic
CATATTCTTGCAATCTGACA+AGG 0.595122 2.1:-56262510 MS.gene00323:CDS
AATTGCACAACAGTGGCTCG+TGG 0.602758 2.1:+56262026 None:intergenic
ATGCCTTTAAGAAATGGGGT+TGG 0.606718 2.1:-56262220 MS.gene00323:CDS
TGAGATAGATTAAGGTTCTG+TGG 0.615734 2.1:+56262987 None:intergenic
GTTGGGAGTTAGAGAAACCA+TGG 0.615746 2.1:+56262723 None:intergenic
CATCAACAACCAAAACCTTG+TGG 0.615914 2.1:-56262256 MS.gene00323:CDS
AGACATGCTCAAGTTGGTGA+AGG 0.618045 2.1:-56262342 MS.gene00323:CDS
AATGTTGGAAAAGAGGGTTA+GGG 0.620954 2.1:+56262927 None:intergenic
GGCTTACAGACAAGACGCCA+GGG 0.643701 2.1:+56262570 None:intergenic
ATCAACTTTCTCATTCCACA+AGG 0.645223 2.1:+56262241 None:intergenic
GAGAAGAAATTGCACAACAG+TGG 0.646434 2.1:+56262019 None:intergenic
AGAACCTTAATCTATCTCAA+GGG 0.657921 2.1:-56262983 MS.gene00323:CDS
AACCTTGTGTGTAAGAACCC+TGG 0.659854 2.1:-56262587 MS.gene00323:CDS
ACTACGTCGTATCATTCCCA+AGG 0.661929 2.1:-56262702 MS.gene00323:CDS
GTATCATTCCCAAGGCACCA+TGG 0.712393 2.1:-56262694 MS.gene00323:CDS
ATGGATGTTATCATACAGTG+AGG 0.724940 2.1:+56262161 None:intergenic
ACTCAAGCTTGCATACACCA+GGG 0.735651 2.1:-56261865 MS.gene00323:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CACTAAATTAATTTTATCTA+TGG + chr2.1:56262728-56262747 None:intergenic 15.0%
!! AAACATTAAAAGATTGCTTA+GGG - chr2.1:56262477-56262496 MS.gene00323:CDS 20.0%
!! AAACTTAATGACAAAATATC+AGG + chr2.1:56262805-56262824 None:intergenic 20.0%
!! TTATCTTATAAAGTTGTATC+AGG - chr2.1:56262975-56262994 MS.gene00323:CDS 20.0%
!!! AATCATTCTAAAACAACTTT+TGG - chr2.1:56262568-56262587 MS.gene00323:CDS 20.0%
!!! TGATATTTTGTCATTAAGTT+TGG - chr2.1:56262804-56262823 MS.gene00323:CDS 20.0%
! CAAACATTAAAAGATTGCTT+AGG - chr2.1:56262476-56262495 MS.gene00323:CDS 25.0%
! GAGAGAAAAAATTGAAACTT+TGG + chr2.1:56262232-56262251 None:intergenic 25.0%
! GCTTAATTTATCCATTCTTA+AGG - chr2.1:56262899-56262918 MS.gene00323:CDS 25.0%
! TGTCATTAAGTTTGGAAAAA+AGG - chr2.1:56262812-56262831 MS.gene00323:CDS 25.0%
!! CAATCTTTTAATGTTTGAGT+GGG + chr2.1:56262473-56262492 None:intergenic 25.0%
!! TAATTTAGTGATGAGCTTTT+GGG - chr2.1:56262738-56262757 MS.gene00323:CDS 25.0%
!! TTAATTTAGTGATGAGCTTT+TGG - chr2.1:56262737-56262756 MS.gene00323:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG - chr2.1:56262235-56262254 MS.gene00323:CDS 25.0%
AAAAGAGAAATGGGTAATGT+TGG + chr2.1:56261958-56261977 None:intergenic 30.0%
AAATGGAGAGAAAAGAGAAA+TGG + chr2.1:56261968-56261987 None:intergenic 30.0%
AAGAAATTGAGAACTGAATC+AGG + chr2.1:56262118-56262137 None:intergenic 30.0%
AATGGAGAGAAAAGAGAAAT+GGG + chr2.1:56261967-56261986 None:intergenic 30.0%
AGAAATTGAGAACTGAATCA+GGG + chr2.1:56262117-56262136 None:intergenic 30.0%
AGAACCTTAATCTATCTCAA+GGG - chr2.1:56261884-56261903 MS.gene00323:CDS 30.0%
GAATATGTACACCAATTCAT+AGG + chr2.1:56262344-56262363 None:intergenic 30.0%
GTGAAGGAATTAAAGGATTT+GGG - chr2.1:56262541-56262560 MS.gene00323:CDS 30.0%
GTGCAATTTCTTCTCAATAA+TGG - chr2.1:56262856-56262875 MS.gene00323:CDS 30.0%
TAATCCCTTGAGATAGATTA+AGG + chr2.1:56261891-56261910 None:intergenic 30.0%
TGAAGGAATTAAAGGATTTG+GGG - chr2.1:56262542-56262561 MS.gene00323:CDS 30.0%
! AGTTGATGCCTTTAAGAAAT+GGG - chr2.1:56262642-56262661 MS.gene00323:CDS 30.0%
! CATACCTCATTCACAATTTT+GGG - chr2.1:56262026-56262045 MS.gene00323:CDS 30.0%
! CTCAATTTCTTTAGAAACCA+TGG - chr2.1:56262127-56262146 MS.gene00323:CDS 30.0%
! GCAATCTTTTAATGTTTGAG+TGG + chr2.1:56262474-56262493 None:intergenic 30.0%
! GTTTAGGGATTGTGATTTTT+GGG + chr2.1:56261921-56261940 None:intergenic 30.0%
! TCATACCTCATTCACAATTT+TGG - chr2.1:56262025-56262044 MS.gene00323:CDS 30.0%
! TGACAAAATATCAGGTGTTT+TGG + chr2.1:56262797-56262816 None:intergenic 30.0%
! TGTTCAAGTTCAACAATACA+TGG - chr2.1:56261855-56261874 MS.gene00323:CDS 30.0%
! TTTAGGGATTGTGATTTTTG+GGG + chr2.1:56261920-56261939 None:intergenic 30.0%
!! AAGTTGATGCCTTTAAGAAA+TGG - chr2.1:56262641-56262660 MS.gene00323:CDS 30.0%
AAAGTTTGGAAGCAGATTGT+GGG + chr2.1:56262057-56262076 None:intergenic 35.0%
AACTTCTCAGACCTTAAGAA+TGG + chr2.1:56262913-56262932 None:intergenic 35.0%
AATAGTCCCAACCTATGAAT+TGG - chr2.1:56262330-56262349 MS.gene00323:CDS 35.0%
AATGGGTAATGTTGGAAAAG+AGG + chr2.1:56261950-56261969 None:intergenic 35.0%
AATGTTGGAAAAGAGGGTTA+GGG + chr2.1:56261943-56261962 None:intergenic 35.0%
ATCAACTTTCTCATTCCACA+AGG + chr2.1:56262629-56262648 None:intergenic 35.0%
ATGGATGTTATCATACAGTG+AGG + chr2.1:56262709-56262728 None:intergenic 35.0%
ATGGGTAATGTTGGAAAAGA+GGG + chr2.1:56261949-56261968 None:intergenic 35.0%
ATGTACACCAATTCATAGGT+TGG + chr2.1:56262340-56262359 None:intergenic 35.0%
CAGAACCTTAATCTATCTCA+AGG - chr2.1:56261883-56261902 MS.gene00323:CDS 35.0%
CATATTCTTGCAATCTGACA+AGG - chr2.1:56262357-56262376 MS.gene00323:CDS 35.0%
GGTGAAGGAATTAAAGGATT+TGG - chr2.1:56262540-56262559 MS.gene00323:CDS 35.0%
TAATGTTGGAAAAGAGGGTT+AGG + chr2.1:56261944-56261963 None:intergenic 35.0%
TGAGATAGATTAAGGTTCTG+TGG + chr2.1:56261883-56261902 None:intergenic 35.0%
TGTACACCAATTCATAGGTT+GGG + chr2.1:56262339-56262358 None:intergenic 35.0%
TTGGGACTATTTGATTCTTG+AGG + chr2.1:56262321-56262340 None:intergenic 35.0%
! ATCACAATGCTGATTGAGAA+TGG - chr2.1:56262442-56262461 MS.gene00323:CDS 35.0%
! GCAATCTGACAAGGAAATTT+TGG - chr2.1:56262366-56262385 MS.gene00323:CDS 35.0%
! GGTTTAGGGATTGTGATTTT+TGG + chr2.1:56261922-56261941 None:intergenic 35.0%
! GTTGATGCCTTTAAGAAATG+GGG - chr2.1:56262643-56262662 MS.gene00323:CDS 35.0%
! TTTCTCATTCCACAAGGTTT+TGG + chr2.1:56262623-56262642 None:intergenic 35.0%
!! CAAGTTGGTGAAGGAATTAA+AGG - chr2.1:56262534-56262553 MS.gene00323:CDS 35.0%
!! TTAGGGATTGTGATTTTTGG+GGG + chr2.1:56261919-56261938 None:intergenic 35.0%
!!! AATATCAGGTGTTTTGGCAA+TGG + chr2.1:56262791-56262810 None:intergenic 35.0%
ATTGCTTAGGGAACATACTC+GGG - chr2.1:56262489-56262508 MS.gene00323:CDS 40.0%
CAAAGTTTGGAAGCAGATTG+TGG + chr2.1:56262058-56262077 None:intergenic 40.0%
CATCAACAACCAAAACCTTG+TGG - chr2.1:56262611-56262630 MS.gene00323:CDS 40.0%
CTTGTAACTGGAGCAAAGTT+TGG + chr2.1:56262071-56262090 None:intergenic 40.0%
GAAAAGAGGGTTAGGGTTTA+GGG + chr2.1:56261936-56261955 None:intergenic 40.0%
GAGAAGAAATTGCACAACAG+TGG + chr2.1:56262851-56262870 None:intergenic 40.0%
GATTGCTTAGGGAACATACT+CGG - chr2.1:56262488-56262507 MS.gene00323:CDS 40.0%
! AAACTTTGGCAACACCCTTT+TGG + chr2.1:56262218-56262237 None:intergenic 40.0%
! ATGCCTTTAAGAAATGGGGT+TGG - chr2.1:56262647-56262666 MS.gene00323:CDS 40.0%
! TGAGAACTGAATCAGGGTTT+TGG + chr2.1:56262111-56262130 None:intergenic 40.0%
!! AGCTTTTGGGTCAATCAGTT+GGG - chr2.1:56262751-56262770 MS.gene00323:CDS 40.0%
!! GGTTTTGGTGAGTCTTGAAA+CGG + chr2.1:56262096-56262115 None:intergenic 40.0%
AACCTTGTGTGTAAGAACCC+TGG - chr2.1:56262280-56262299 MS.gene00323:CDS 45.0%
AACTCAAGCTTGCATACACC+AGG - chr2.1:56263001-56263020 MS.gene00323:CDS 45.0%
ACTACGTCGTATCATTCCCA+AGG - chr2.1:56262165-56262184 MS.gene00323:CDS 45.0%
ACTCAAGCTTGCATACACCA+GGG - chr2.1:56263002-56263021 MS.gene00323:CDS 45.0%
CGACCAACCCCATTTCTTAA+AGG + chr2.1:56262653-56262672 None:intergenic 45.0%
CGAGCCCAAAATTGTGAATG+AGG + chr2.1:56262033-56262052 None:intergenic 45.0%
CTTGAAACGGAGCTTGTAAC+TGG + chr2.1:56262083-56262102 None:intergenic 45.0%
GAGGTATGAGACAGCAAAAG+AGG + chr2.1:56262014-56262033 None:intergenic 45.0%
GGAAAAGAGGGTTAGGGTTT+AGG + chr2.1:56261937-56261956 None:intergenic 45.0%
GGGAATGATACGACGTAGTT+GGG + chr2.1:56262164-56262183 None:intergenic 45.0%
GTTGGGAGTTAGAGAAACCA+TGG + chr2.1:56262147-56262166 None:intergenic 45.0%
TGGGAATGATACGACGTAGT+TGG + chr2.1:56262165-56262184 None:intergenic 45.0%
TTGGGTCAATCAGTTGGGTT+GGG - chr2.1:56262756-56262775 MS.gene00323:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG - chr2.1:56262755-56262774 MS.gene00323:CDS 45.0%
! AGAGGTTGAATCAGAAGCGT+TGG + chr2.1:56261996-56262015 None:intergenic 45.0%
! TTTGGCAACACCCTTTTGGA+GGG + chr2.1:56262214-56262233 None:intergenic 45.0%
!! AGACATGCTCAAGTTGGTGA+AGG - chr2.1:56262525-56262544 MS.gene00323:CDS 45.0%
!! GAGCTTTTGGGTCAATCAGT+TGG - chr2.1:56262750-56262769 MS.gene00323:CDS 45.0%
AATTGCACAACAGTGGCTCG+TGG + chr2.1:56262844-56262863 None:intergenic 50.0%
GACACGAGACATGCTCAAGT+TGG - chr2.1:56262519-56262538 MS.gene00323:CDS 50.0%
! CTTTGGCAACACCCTTTTGG+AGG + chr2.1:56262215-56262234 None:intergenic 50.0%
! GTATCATTCCCAAGGCACCA+TGG - chr2.1:56262173-56262192 MS.gene00323:CDS 50.0%
!! CAGAAGCGTTGGTGCAGAAA+TGG + chr2.1:56261985-56262004 None:intergenic 50.0%
!! GTCAGAAGTAGAAGCGCCTT+TGG + chr2.1:56262254-56262273 None:intergenic 50.0%
!! TCAGTTGGGTTGGGATGCTA+TGG - chr2.1:56262765-56262784 MS.gene00323:CDS 50.0%
!! TTGGCAACACCCTTTTGGAG+GGG + chr2.1:56262213-56262232 None:intergenic 50.0%
AGGCTTACAGACAAGACGCC+AGG + chr2.1:56262301-56262320 None:intergenic 55.0%
CGCCAGGGTTCTTACACACA+AGG + chr2.1:56262285-56262304 None:intergenic 55.0%
GGAAGTAGCCATGGTGCCTT+GGG + chr2.1:56262184-56262203 None:intergenic 55.0%
GGCTTACAGACAAGACGCCA+GGG + chr2.1:56262300-56262319 None:intergenic 55.0%
GGGAAGTAGCCATGGTGCCT+TGG + chr2.1:56262185-56262204 None:intergenic 60.0%
TCCCTGCGACCCCTCCAAAA+GGG - chr2.1:56262201-56262220 MS.gene00323:CDS 60.0%
TTCCCTGCGACCCCTCCAAA+AGG - chr2.1:56262200-56262219 MS.gene00323:CDS 60.0%
!! ACCCTTTTGGAGGGGTCGCA+GGG + chr2.1:56262205-56262224 None:intergenic 60.0%
GGGTCGCAGGGAAGTAGCCA+TGG + chr2.1:56262193-56262212 None:intergenic 65.0%
!! CACCCTTTTGGAGGGGTCGC+AGG + chr2.1:56262206-56262225 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 56261854 56263035 56261854 ID=MS.gene00323
chr2.1 mRNA 56261854 56263035 56261854 ID=MS.gene00323.t1;Parent=MS.gene00323
chr2.1 exon 56261854 56263035 56261854 ID=MS.gene00323.t1.exon1;Parent=MS.gene00323.t1
chr2.1 CDS 56261854 56263035 56261854 ID=cds.MS.gene00323.t1;Parent=MS.gene00323.t1
Gene Sequence

>MS.gene00323

ATGTTCAAGTTCAACAATACATGGCGCCACAGAACCTTAATCTATCTCAAGGGATTAACTTACCCCCAAAAATCACAATCCCTAAACCCTAACCCTCTTTTCCAACATTACCCATTTCTCTTTTCTCTCCATTTCTGCACCAACGCTTCTGATTCAACCTCTTTTGCTGTCTCATACCTCATTCACAATTTTGGGCTCGACCCACAATCTGCTTCCAAACTTTGCTCCAGTTACAAGCTCCGTTTCAAGACTCACCAAAACCCTGATTCAGTTCTCAATTTCTTTAGAAACCATGGTTTCTCTAACTCCCAACTACGTCGTATCATTCCCAAGGCACCATGGCTACTTCCCTGCGACCCCTCCAAAAGGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGCGCTTCTACTTCTGACATTGTTAACCTTGTGTGTAAGAACCCTGGCGTCTTGTCTGTAAGCCTCAAGAATCAAATAGTCCCAACCTATGAATTGGTGTACATATTCTTGCAATCTGACAAGGAAATTTTGGCATTGTTGAATTACAATACAAATTTACTTTGTGATCGTACTGTGCATAATAACATCACAATGCTGATTGAGAATGGAGTGTCCCACTCAAACATTAAAAGATTGCTTAGGGAACATACTCGGGCATTCAAGACACGAGACATGCTCAAGTTGGTGAAGGAATTAAAGGATTTGGGGTTTAATCATTCTAAAACAACTTTTGGAGTAGCATTGATAGCAAAATCATCAACAACCAAAACCTTGTGGAATGAGAAAGTTGATGCCTTTAAGAAATGGGGTTGGTCGAATGAAGATGTTCTTCAAGCATTTAGAAAGCAGCCTCACTGTATGATAACATCCATAGATAAAATTAATTTAGTGATGAGCTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCCAAAACACCTGATATTTTGTCATTAAGTTTGGAAAAAAGGATCATTCCACGAGCCACTGTTGTGCAATTTCTTCTCAATAATGGTTTGCGAAGTAAGAATTCAAGCTTAATTTATCCATTCTTAAGGTCTGAGAAGTTGTTTCTTGATATGTTTATAAAACGTTTTAAGAATGAGTCTTCTTATCTTATAAAGTTGTATCAGGAAAAACTCAAGCTTGCATACACCAGGGACAAGACATGA
Protein sequence

>MS.gene00323.t1

MFKFNNTWRHRTLIYLKGLTYPQKSQSLNPNPLFQHYPFLFSLHFCTNASDSTSFAVSYLIHNFGLDPQSASKLCSSYKLRFKTHQNPDSVLNFFRNHGFSNSQLRRIIPKAPWLLPCDPSKRVLPKFQFFLSKGASTSDIVNLVCKNPGVLSVSLKNQIVPTYELVYIFLQSDKEILALLNYNTNLLCDRTVHNNITMLIENGVSHSNIKRLLREHTRAFKTRDMLKLVKELKDLGFNHSKTTFGVALIAKSSTTKTLWNEKVDAFKKWGWSNEDVLQAFRKQPHCMITSIDKINLVMSFWVNQLGWDAMAIAKTPDILSLSLEKRIIPRATVVQFLLNNGLRSKNSSLIYPFLRSEKLFLDMFIKRFKNESSYLIKLYQEKLKLAYTRDKT